Quercetin-Containing Extract from Clove Syzygium aromaticum L. Endophytic Bacteria, Fictibacillus phosphorivorans P1U2, Exhibits Antimutagenic Activity in Yeast Saccharomyces cerevisiae

Q3 Agricultural and Biological Sciences
Nadila Fany Ashafani, R. Astuti, S. Listiyowati, Irmanida Batubara
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引用次数: 0

Abstract

Metabolites from clove (Syzygium aromaticum L.) have been extensively studied for various pharmacological uses, including quercetin. Endophytic bacteria isolated from plant tissues can produce similar secondary metabolites as their host plants. However, little is known about the antioxidant activity of the endophytic bacterial extract from clove leaves. Amongst the potential bacterial groups, the genera of Bacillus have been widely studied as a source of antioxidants. Thus, this study, we examined the potential antioxidant properties of Bacillus sp., isolated from clove leaves. We have successfully isolated ten endophytic bacteria, of which only one isolate (P1U2) was found non-pathogenic based on a hemolytic assay. Based on the 16S rRNA sequence, P1U2 shared the highest similarity to Fictibacillus phosphorivorans. The extract of extracellular metabolites of P1U2 contains quercetin compounds, based on LC-MS analysis. The results of the antioxidant activity test using the DPPH assay showed that the P1U2 extract had an IC50 value of 161 mg/ml. The supplementation of the extract on culture media reduced the ability of S. cerevisiae to decolorize mutagen malachite green and increased cell viability. Furthermore, the addition of 161 ppm extract was able to counteract UV mutagens effects of UV exposure and toxicity of malachite green, significantly increasing the growth of S. cerevisiae. Suggesting the bioactivity of the extract in combating mutagens. To the best of our knowledge, this is the first study to report a quercetin-rich extract derived from endophytic bacteria, F. phosphorivorans. Thus, further study is required to optimize the synthesis of quercetin from isolate P1U2.
含槲皮素的丁香香菇内生细菌P1U2对酵母的抗诱变活性研究
丁香(Syzygium aromaticum L.)的代谢物已被广泛研究用于各种药理用途,包括槲皮素。从植物组织中分离出来的内生细菌可以产生与宿主植物相似的次生代谢物。然而,人们对丁香叶内生细菌提取物的抗氧化活性知之甚少。在潜在的细菌群中,芽孢杆菌属作为抗氧化剂的来源已被广泛研究。因此,在这项研究中,我们检测了从丁香叶中分离的芽孢杆菌的潜在抗氧化特性。我们已经成功地分离出10种内生细菌,其中只有一种(P1U2)根据溶血试验被发现是非致病性的。基于16S rRNA序列,P1U2与嗜磷芽孢杆菌的相似性最高。经LC-MS分析,P1U2细胞外代谢产物提取物中含有槲皮素类化合物。DPPH法测定P1U2提取物的抗氧化活性,IC50值为161 mg/ml。在培养基中添加提取物降低了酿酒酵母对诱变剂孔雀石绿的脱色能力,提高了细胞活力。此外,添加161 ppm提取物能够抵消紫外线照射和孔雀石绿毒性的紫外线诱变效应,显著促进酿酒酵母的生长。提示提取物具有抗诱变剂的生物活性。据我们所知,这是第一次报道从内生细菌F. phosphorivorans中提取的富含槲皮素的提取物。因此,从分离物P1U2中优化槲皮素的合成还需进一步研究。
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来源期刊
HAYATI Journal of Biosciences
HAYATI Journal of Biosciences Agricultural and Biological Sciences-Agricultural and Biological Sciences (all)
CiteScore
1.10
自引率
0.00%
发文量
75
审稿时长
24 weeks
期刊介绍: HAYATI Journal of Biosciences (HAYATI J Biosci) is an international peer-reviewed and open access journal that publishes significant and important research from all area of biosciences fields such as biodiversity, biosystematics, ecology, physiology, behavior, genetics and biotechnology. All life forms, ranging from microbes, fungi, plants, animals, and human, including virus, are covered by HAYATI J Biosci. HAYATI J Biosci published by Department of Biology, Bogor Agricultural University, Indonesia and the Indonesian Society for Biology. We accept submission from all over the world. Our Editorial Board members are prominent and active international researchers in biosciences fields who ensure efficient, fair, and constructive peer-review process. All accepted articles will be published on payment of an article-processing charge, and will be freely available to all readers with worldwide visibility and coverage.
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