HAYATI Journal of Biosciences最新文献

Expression of Immunoglobulin M (IgM) and Immunoglobulin G (IgG) in Normal Wistar Rat Post-Cheral® Administration 服用 Chheral® 后正常 Wistar 大鼠体内免疫球蛋白 M (IgM) 和免疫球蛋白 G (IgG) 的表达情况

HAYATI Journal of Biosciences Pub Date : 2024-07-10 DOI: 10.4308/hjb.31.5.1030-1036

Firda Nuri Asyhari, Heni Sukma Zulfatim, Nenis Try Melani Putri, Moh Dliyauddin, Ahmad Shobrun Jamil, A. Soewondo, Muhammad Halim Natsir, Mansur Ibrahim, Sri Rahayu, M. S. Djati, M. Rifa’i

{"title":"Expression of Immunoglobulin M (IgM) and Immunoglobulin G (IgG) in Normal Wistar Rat Post-Cheral® Administration","authors":"Firda Nuri Asyhari, Heni Sukma Zulfatim, Nenis Try Melani Putri, Moh Dliyauddin, Ahmad Shobrun Jamil, A. Soewondo, Muhammad Halim Natsir, Mansur Ibrahim, Sri Rahayu, M. S. Djati, M. Rifa’i","doi":"10.4308/hjb.31.5.1030-1036","DOIUrl":"https://doi.org/10.4308/hjb.31.5.1030-1036","url":null,"abstract":"Maintaining immunoglobulin levels in the body is important to protect the body from exposure to pathogens. One effort can be made by consuming herbs containing immunomodulatory compounds, such as Cheral®, which includes a combination of herbs Phyllanthus niruri and Curcuma longa. This research aims to determine the expression of immunoglobulin M (IgM) and immunoglobulin G (IgG) following the administration of Cheral® to Wistar rats. The study was conducted in vivo, utilizing 24 healthy male Wistar rats for a 90-day treatment period. The research was divided into four treatment groups, including a control group and three dosage groups: Dose 1 (156.25 mg/kg BW), Dose 2 (312.5 mg/kg BW), and Dose 3 (468.75 mg/kg BW). IgM and IgG were isolated from the spleen and analyzed using flow cytometry. Flow cytometry data were analyzed using SPSS with a one-way ANOVA and post hoc test (p-value <0.05). The analysis showed that the relative number of IgM-producing cells in the control group was significantly higher than in the treatment groups, with a difference of 44.40%. In contrast, the relative number of IgG-producing cells in Dose 3 was significantly lower than all other treatment groups, showing a decrease of 29.21%. Overall, the expression of IgG and IgM did not differ substantially across all treatments. The lower IgG and IgM profiles compared to the control group indicate Cheral®'s ability to prevent infections and maintain the immune system of the rats throughout the treatment period.","PeriodicalId":12927,"journal":{"name":"HAYATI Journal of Biosciences","volume":"31 15","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141660519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic Relationship and the Putative Occurrence of A Species Complex Within the Indonesian Calotes (Daudin, 1802) (Squamata, Agamidae) Genus Based on COI Gene Sequences 基于 COI 基因序列的印度尼西亚 Calotes（Daudin，1802 年）（有鳞目，蟾蜍科）属内部的遗传关系和一个物种复合体的存在可能性

HAYATI Journal of Biosciences Pub Date : 2024-07-05 DOI: 10.4308/hjb.31.5.1019-1029

Muhammad Indra Maulana, S. Pakpahan, Andy Darmawan, Yanti Ariyanti

{"title":"Genetic Relationship and the Putative Occurrence of A Species Complex Within the Indonesian Calotes (Daudin, 1802) (Squamata, Agamidae) Genus Based on COI Gene Sequences","authors":"Muhammad Indra Maulana, S. Pakpahan, Andy Darmawan, Yanti Ariyanti","doi":"10.4308/hjb.31.5.1019-1029","DOIUrl":"https://doi.org/10.4308/hjb.31.5.1019-1029","url":null,"abstract":"The Calotes genus presents a challenge due to the complexity of its species. However, research on the cryptic species complex within the Indonesian Calotes genus is still lacking. This study aims to determine the extent of genetic relationships and assess the potential existence of a species complex within the Indonesian genus Calotes (Daudin, 1802) (Squamata, Agamidae) using the partial Cytochrome c Oxidase Subunit 1 (COI) gene sequence as a molecular marker. Samples of the Indonesian Calotes genus in this study were collected from South Lampung (Lampung), Bogor (West Java), and Langkat (North Sumatra). By aligning 582 bp sequence similarities with reference sequences in GenBank, we confirmed that seven out of eight samples analyzed belonged to Calotes vultuosus, while one sample was identified as Calotes versicolor. The identity values ranged from 96 to 100%. The C. vultuosus samples in this study displayed lower genetic distances, ranging from 0 to 3%, with the reference C. vultuosus sequence from Indonesia compared to the reference sequence from India, which ranged from 6 to 9%. Phylogenetic tree reconstruction, utilizing both maximum likelihood with IQ-Tree and Bayesian Inference with BEAST methods, further supports these findings. It reveals distinct groupings between C. vultuosus samples from Indonesia and India. These results suggest the potential occurrence of a species complex within the Indonesian genus Calotes. Furthermore, the inclusion of eight COI gene sequences from two Calotes species in the GenBank database has the potential to confirm the existence of previously undocumented species in Indonesia.","PeriodicalId":12927,"journal":{"name":"HAYATI Journal of Biosciences","volume":" 29","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141673699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In Silico Study, Design, and Expression of an Intranasal Dual Chimeric Vaccine for Indonesian-Based Norovirus GII-2 and Hepatitis B 针对印度尼西亚诺如病毒 GII-2 和乙型肝炎的鼻内双嵌合疫苗的硅学研究、设计和表达

HAYATI Journal of Biosciences Pub Date : 2024-07-03 DOI: 10.4308/hjb.31.5.1007-1018

E. Giri-rachman, Marselina Irasonia Tan, Novia Syari Intan, Putri Ayu Fajar, Gladys Emmanuella, Putri Wojciechowska, R. Hertadi, Debbie Soefie

{"title":"In Silico Study, Design, and Expression of an Intranasal Dual Chimeric Vaccine for Indonesian-Based Norovirus GII-2 and Hepatitis B","authors":"E. Giri-rachman, Marselina Irasonia Tan, Novia Syari Intan, Putri Ayu Fajar, Gladys Emmanuella, Putri Wojciechowska, R. Hertadi, Debbie Soefie","doi":"10.4308/hjb.31.5.1007-1018","DOIUrl":"https://doi.org/10.4308/hjb.31.5.1007-1018","url":null,"abstract":"Hepatitis B virus (HBV) remains an important healthcare challenge, leading to liver diseases like cirrhosis and cancer. In response, we created a prophylactic and therapeutic HBV vaccine by integrating HBcAg and HBsAg from HBV genotype B into Norovirus (NoV) GII.2 P domain (PdomGII.2-HBV) for enhanced intranasal delivery. This vaccine also aimed to simultaneously prevent NoV infection, which causes gastroenteritis. Since the selected HBV epitopes have undergone extensive research and are tailored to the Indonesian population, this study focused on identifying NoV epitopes and assessing T cell epitopes coverage of the PdomGII.2-HBV for the Indonesian population. Following that, we expressed the PdomGII.2-HBV protein using Escherichia coli BL21(DE3) and employed a gentle solubilization technique for protein purification. Our in-silico analysis identified two B cell epitopes, along with 15 CD4+T cell epitopes and 35 CD8+T cell epitopes within the GII.2 P domain. These T cell epitopes cover 100% of the Javanese-Sundanese population's HLA allele variations, which constituted the largest demographic group in Indonesia. Subsequently, we successfully purified the presumed PdomGII.2-HBV protein, revealing a molecular weight of 39.5 kDa. Following the successful expression and purification of the presumed PdomGII.2-HBV protein, it is evident that this vaccine design has significant potential, warranting further study.","PeriodicalId":12927,"journal":{"name":"HAYATI Journal of Biosciences","volume":" 24","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141681027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Potential of Clitoria ternatea L. Extract Towards Insulin Receptor Expression and Marker of Inflammation in Diabetes Mellitus Rats Model 蝎尾草提取物对糖尿病大鼠模型中胰岛素受体表达和炎症标记物的潜在影响

HAYATI Journal of Biosciences Pub Date : 2024-04-04 DOI: 10.4308/hjb.31.4.803-810

Djaja Rusmana, H. Tiono, Wahyu Widowati, Johan Lucianus, Lusiana Darsono, Philips Onggowidjaja, R. Tjokropranoto, Vera Vera, Edwin Setiabudi, S. Obeng, H. Kusuma, Agung Novianto

{"title":"Potential of Clitoria ternatea L. Extract Towards Insulin Receptor Expression and Marker of Inflammation in Diabetes Mellitus Rats Model","authors":"Djaja Rusmana, H. Tiono, Wahyu Widowati, Johan Lucianus, Lusiana Darsono, Philips Onggowidjaja, R. Tjokropranoto, Vera Vera, Edwin Setiabudi, S. Obeng, H. Kusuma, Agung Novianto","doi":"10.4308/hjb.31.4.803-810","DOIUrl":"https://doi.org/10.4308/hjb.31.4.803-810","url":null,"abstract":"Metabolic abnormalities caused by the accumulation of human, environmental, genetic and lifestyle variables can be found in diabetes mellitus (DM). An increase in blood glucose carried on by a reduction in insulin production can also result in DM. Insulin resistance often occurs as a result of obesity and a lack of physical activity and aging. Telang flower extract (Clitoria ternatea L.) is reported to have several qualities such as being able to treat DM, chronic bronchitis, goiter, mucosal disorders and leprosy. This study’s objective was to elucidate anti-dibetic effect of C. ternatea extract (CTE) in rats with diabetic complications related to dyslipidemia. Rats suffering from diabetes will be examined after being given oral doses of Simvastatin 0.9 mg/kg BW, Glibenclamide 0.45 mg/kg BW, and 200, 400, and 800 mg/kg BW CTE, model induced by streptozotocin. The expression of the insulin gene (INS-1) was investigated by qRTPCR, Tumor Necrosis Factor-α (TNF-α), and Interleukin-1β (IL-1β) pancreatic DM rats model using the Immunohistochemistry (IHC) test. Both descriptive and quantitative data were acquired for the data. After utilizing ANOVA to evaluate quantitative data, the Tukey post hoc test was used to analyze the data, the expression of TNF-α and IL-1β was found to decrease while INS-1 expression increased in response to CTE. This effect was attributed to the modulation of TNF-α, IL-1β, and INS-1 expression. These findings suggest that CTE possesses antidiabetic properties.","PeriodicalId":12927,"journal":{"name":"HAYATI Journal of Biosciences","volume":"3 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140741795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genomics and Phylogeny of Rhodotorula glutinis and Rhodotorula kratochvilovae Isolated from the Northern Peruvian Andes 从秘鲁安第斯山脉北部分离出的糯米花癣菌（Rhodotorula glutinis）和克拉托奇维洛夫癣菌（Rhodotorula kratochvilovae）的基因组学和系统发育

HAYATI Journal of Biosciences Pub Date : 2024-04-04 DOI: 10.4308/hjb.31.4.793-802

Víctor Javier Vásquez Villalobos, Angely Meryl Alessandra Hidalgo-Arteaga, Roxana Beatriz Sosa-Becerra, Bertha Soledad Soriano-Bernilla, Maurício Alexander de Moura Ferreira, Wendel Batista da Silveira

{"title":"Genomics and Phylogeny of Rhodotorula glutinis and Rhodotorula kratochvilovae Isolated from the Northern Peruvian Andes","authors":"Víctor Javier Vásquez Villalobos, Angely Meryl Alessandra Hidalgo-Arteaga, Roxana Beatriz Sosa-Becerra, Bertha Soledad Soriano-Bernilla, Maurício Alexander de Moura Ferreira, Wendel Batista da Silveira","doi":"10.4308/hjb.31.4.793-802","DOIUrl":"https://doi.org/10.4308/hjb.31.4.793-802","url":null,"abstract":"Genomes of oleaginous yeast strains Rhodotorula glutinis CON-5 and Rhodotorula kratochvilovae POR-3, isolated from areas in the northern Peruvian Andes using SPAdes, were sequenced and assembled applying Illumina and de novo. Genomes of 20,515,696 and 20,738,185 bp, respectively, were determined. From the structural and functional annotations, the Basidiomycota phylum showed a similarity of 76.8% and 86.5% with 6,976 and 8,124 pairs of proteins in both yeasts respectively, with homologues in the UniProt data bank. Using OrthoVenn, a relationship between both yeasts was obtained from 450 orthologous groups. Likewise, the above-mentioned yeasts and R. toruloides (oleaginous Basidiomycota) showed 1,574 orthologous groups, indicating a good relationship. Construction of phylogenetic trees of genes encoding metabolic enzymes was also carried out, based on the ITS sequences which showed that CON-5 and POR-3 have a greater relationship with R. graminis. Their phylogenetic relationship was ascertained and determined that the enzymes involved in the metabolism of CON-5 and POR-3 are related to each other. It was also found that the protein sequences of the Basidiomycota phylum differ from Ascomycota. The study showed functional evidence regarding the lipid accumulation phenotype, an important aspect in the context of obtaining lipids or oleochemicals.","PeriodicalId":12927,"journal":{"name":"HAYATI Journal of Biosciences","volume":"2 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140742317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phenotypic and Estimated Genetic Variability in Endemic Diospyros celebica Bakh. and Widely-spread D. blancoi A.Dc. Cultivated at Purwodadi Botanic Garden, Indonesia 在印度尼西亚普沃达迪植物园栽培的特有种 Diospyros celebica Bakh.和广布种 Diospyros blancoi A.Dc. 的表型和估计遗传变异性在印度尼西亚 Purwodadi 植物园栽培的 D.

HAYATI Journal of Biosciences Pub Date : 2024-04-03 DOI: 10.4308/hjb.31.4.781-792

I. K. Abywijaya, R. Rindyastuti, Fira Fitria Jihans, A. T. Wibowo

{"title":"Phenotypic and Estimated Genetic Variability in Endemic Diospyros celebica Bakh. and Widely-spread D. blancoi A.Dc. Cultivated at Purwodadi Botanic Garden, Indonesia","authors":"I. K. Abywijaya, R. Rindyastuti, Fira Fitria Jihans, A. T. Wibowo","doi":"10.4308/hjb.31.4.781-792","DOIUrl":"https://doi.org/10.4308/hjb.31.4.781-792","url":null,"abstract":"The endemic Diospyros celebica and widely spread D. blancoi are two valuable species of Diospyros (the genus of persimmons and ebonies) that have been conserved at botanic gardens in Indonesia. Understanding the biological contrasts between endemic and widely spread species is essential for plant conservation. This study aims to compare the phenotypic and estimated genetic variability between the endemic D. celebica and the widely spread D. blancoi to support their conservation. Fifty-three morphological characters from fifteen selected living plant specimens at Purwodadi Botanic Garden were measured directly or using variable encoding methods. Unpaired two-samples Wilcoxon test and hierarchical cluster analysis were used to compare morphological variations between both species. Further, genetic variabilities were estimated from encoded morphological characters using GenAlEx 6.503 software. The two Diospyros’ morphological characters were significantly different, except for the lamina length, pairs of leaf vein, number of flowers in each inflorescence, and the fruit sulci presence. The cluster analysis successfully distinguished D. celebica from D. blancoi according to thirteen comparable morphological characters. The endemic D. celebica has lower genetic variability (female/bisexual %P = 86.21%, He = 0.259, uHe = 0.277; male P = 54.29%, He = 0.195, uHe = 0.234) than the widely spread D. blancoi (female/bisexual %P = 86.76, He = 0.311, uHe = 0.355). Due to the importance of genetic diversity in plant conservation, both species’ population size needs to be maintained, if not increased, to preserve the existing phenotypic and genetic variabilities within the cultivated populations at the botanic garden.","PeriodicalId":12927,"journal":{"name":"HAYATI Journal of Biosciences","volume":"514 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140749552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of Garlic Viruses Associated with Seed Bulbs and Consumption Bulbs from Several Locations in Indonesia 鉴定印度尼西亚多个地点的鳞茎种子和食用鳞茎中的大蒜病毒

HAYATI Journal of Biosciences Pub Date : 2024-04-01 DOI: 10.4308/hjb.31.4.733-743

Sari Nurulita, Sofi Mawarni, S H Hidayat

{"title":"Identification of Garlic Viruses Associated with Seed Bulbs and Consumption Bulbs from Several Locations in Indonesia","authors":"Sari Nurulita, Sofi Mawarni, S H Hidayat","doi":"10.4308/hjb.31.4.733-743","DOIUrl":"https://doi.org/10.4308/hjb.31.4.733-743","url":null,"abstract":"Virus infection is one of the major constraints in garlic production since the viruses are readily accumulated on vegetative propagation material (bulbs). This research aimed to detect garlic common latent virus (GCLV), shallot latent virus (SLV), onion yellow dwarf virus (OYDV), and leek yellow stripe virus (LYSV) infecting local garlic as seed bulb and imported garlic as consumption bulb. Seed bulb samples were obtained from seed breeders in several garlic growing centers in Indonesia. In contrast, consumption bulb samples were obtained from plant quarantine warehouses and three local markets in Bogor. Some bulb samples were used for morphological observations, and some were germinated in the laboratory until the leaves emerged. Leaves were collected for virus detection by RT-PCR using specific primers for GCLV, SLV, OYDV, and LYSV. Seed and consumption bulbs have differences in their morphological characteristics, especially in the type of neck hardness and the size of the bulb diameter. OYDV and LYSV infections were successfully detected in seed and consumption bulbs, while SLV was only found in consumption bulbs. Nucleotide sequence analysis showed that SLV from consumption bulbs formed one group, GCLV from seed bulbs formed one group, while OYDV and LYSV from seed and consumption bulbs were in different groups, indicating that the viruses came from different strains. Further research through high-throughput detection methods and providing virus-free planting material are needed to anticipate the spread of new strains of garlic viruses in Indonesia.","PeriodicalId":12927,"journal":{"name":"HAYATI Journal of Biosciences","volume":"61 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140795522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic Diversity of Flying Fish (Exocoetidae) in Southeast Sulawesi, Indonesia 印度尼西亚东南苏拉威西岛飞鱼（Exocoetidae）的遗传多样性

HAYATI Journal of Biosciences Pub Date : 2024-04-01 DOI: 10.4308/hjb.31.4.744-749

I. Indrayani, Sri Ambardini, A. Pariakan, Indriyani Nur

{"title":"Genetic Diversity of Flying Fish (Exocoetidae) in Southeast Sulawesi, Indonesia","authors":"I. Indrayani, Sri Ambardini, A. Pariakan, Indriyani Nur","doi":"10.4308/hjb.31.4.744-749","DOIUrl":"https://doi.org/10.4308/hjb.31.4.744-749","url":null,"abstract":"Flying fish (Exocoetidae) is a pelagic fishery commodity that holds economic value among the people of eastern Indonesia. Flying fish are advantageous as they are abundant in Indonesian sea waters, making them an affordable source of omega-3, which is beneficial for human health. However, the high demand for flying fish has resulted in overfishing, which poses a risk of reducing their natural population. Proper management of this commodity is necessary to minimize these risks. Effective management requires adequate information, including genetic diversity data. This study aims to determine the genetic diversity of flying fish in Southeast Sulawesi, specifically in the Banda Sea and Bone Bay populations. Tissue samples were collected from flying fish in the two populations during the study in March 2022. The results show that the genetic diversity values of flying fish in Bone Bay and the Banda Sea were 0.984 and 1.00, respectively, while the nucleotide diversity values were 0.021 and 0.018. High genetic diversity values and nucleotide diversity values were found between the two populations, with values of 0.990 and 0.020, respectively. Based on phylogenetic analysis, the two populations in Southeast Sulawesi are genetically similar.","PeriodicalId":12927,"journal":{"name":"HAYATI Journal of Biosciences","volume":"143 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140761937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biosurfactant Activity of Bacillus sp. Strain LP04 Isolate and Its Antifungal Potency against Ganoderma boninense and Fusarium sp. 菌株 LP04 分离物的生物表面活性剂活性及其对骨灵芝和镰刀菌的抗真菌效力

HAYATI Journal of Biosciences Pub Date : 2024-04-01 DOI: 10.4308/hjb.31.4.725-732

Vincentia Fenice Angger Maherani, N. R. Mubarik, J. A. Priyanto, Ivan Permana Putra

{"title":"Biosurfactant Activity of Bacillus sp. Strain LP04 Isolate and Its Antifungal Potency against Ganoderma boninense and Fusarium sp.","authors":"Vincentia Fenice Angger Maherani, N. R. Mubarik, J. A. Priyanto, Ivan Permana Putra","doi":"10.4308/hjb.31.4.725-732","DOIUrl":"https://doi.org/10.4308/hjb.31.4.725-732","url":null,"abstract":"Biosurfactants are a class of amphipathic molecules that various microorganisms can produce. Biosurfactants are used as biopesticides and biocontrol agents because they have antimicrobial activity, especially as antifungal agents in several species of fungal pathogens such as Ganoderma boninense and Fusarium sp. that attack crops. This study aims to detect the biosurfactant activity of Bacillus sp. and its potential as an antifungal agent against the fungi Ganoderma boninense and Fusarium sp. Biosurfactants were produced in mineral salt medium (MSM) by harvesting cell-free supernatants. Screening of biosurfactant-producing isolates was carried out using an oil-spreading assay, a hemolysis assay, and an emulsification index. The antifungal activity of the isolates was then tested using the agar diffusion method. The LP04 isolate was closely related to Bacillus thuringiensis with a 99% similarity level. It has the potential to have biosurfactant activity, which is characterized by a positive result on the oil spreading assay test and has an emulsification index of 48.33±2.87%. The cell-free supernatants of the bacterial isolate were able to inhibit the growth of Ganoderma boninense and Fusarium sp. with growth inhibition rates of 51.11% and 56.92%, respectively.","PeriodicalId":12927,"journal":{"name":"HAYATI Journal of Biosciences","volume":"1301 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140773711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Apis cerana Fabricius, 1793 in Sumatra: Haplotype Variations of Mitochondrial DNA and the Molecular Relationship with the Asian Honey Bees (Hymenoptera: Apidae) 苏门答腊的蜜蜂（Apis cerana Fabricius, 1793）：线粒体 DNA 的单倍型变异以及与亚洲蜜蜂的分子关系（膜翅目：喙蜂科

HAYATI Journal of Biosciences Pub Date : 2024-04-01 DOI: 10.4308/hjb.31.4.768-780

Juniarto Gautama Simanjuntak, W. Priawandiputra, R. Raffiudin, N. I. Shullia, Jauharlina Jauharlina, Mahardika Gama Pradana, Araz Meilin, J. Jasmi, Yulia Pujiastuti, Puji Lestari, Rustem Ilyasov, Rahmadi Sitompul, T. Atmowidi

{"title":"Apis cerana Fabricius, 1793 in Sumatra: Haplotype Variations of Mitochondrial DNA and the Molecular Relationship with the Asian Honey Bees (Hymenoptera: Apidae)","authors":"Juniarto Gautama Simanjuntak, W. Priawandiputra, R. Raffiudin, N. I. Shullia, Jauharlina Jauharlina, Mahardika Gama Pradana, Araz Meilin, J. Jasmi, Yulia Pujiastuti, Puji Lestari, Rustem Ilyasov, Rahmadi Sitompul, T. Atmowidi","doi":"10.4308/hjb.31.4.768-780","DOIUrl":"https://doi.org/10.4308/hjb.31.4.768-780","url":null,"abstract":"Honey bee Apis cerana is widely distributed in Asia and the Indonesian archipelago, including Sumatra. We studied the molecular variations of A. cerana using cytochrome c oxidase subunits 1 and 2 genes (cox1 and cox2) and the cox1/cox2 intergenic spacers (igs) in several altitudes in the six provinces of Sumatra. We explored the haplotype distributions of those three mtDNA markers for A. cerana in the low-, mid-, and highlands of Sumatra. We also analyzed their relationship with A. cerana in Sundaland and Asia using those markers. Our study revealed 12 new haplotypes of A. cerana cox1 in Sumatra, while nine and eight new haplotypes for cox2 and igs, respectively. Apis cerana in North Sumatra, Lampung, and South Sumatra had the three highest haplotype variations. Most of the specific haplotypes of inter-colony A. cerana from Sumatra were found in the lowlands, while most were in the highlands for intra-colony variations. We found low gene flow among populations of A. cerana in Sumatra. One haplotype, Sumatra4 cox2 from North Sumatra, was the same as Java3 haplotype, presumably due to anthropogenic impact. The molecular phylogenetic tree of A. cerana in the Sundaland revealed that A. cerana from Sumatra has a close relationship to those of Borneo compared to Java.","PeriodicalId":12927,"journal":{"name":"HAYATI Journal of Biosciences","volume":"333 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140781054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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