Biosurfactant Activity of Bacillus sp. Strain LP04 Isolate and Its Antifungal Potency against Ganoderma boninense and Fusarium sp.

Q3 Agricultural and Biological Sciences

HAYATI Journal of Biosciences Pub Date : 2024-04-01 DOI:10.4308/hjb.31.4.725-732

Vincentia Fenice Angger Maherani, N. R. Mubarik, J. A. Priyanto, Ivan Permana Putra

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Abstract

Biosurfactants are a class of amphipathic molecules that various microorganisms can produce. Biosurfactants are used as biopesticides and biocontrol agents because they have antimicrobial activity, especially as antifungal agents in several species of fungal pathogens such as Ganoderma boninense and Fusarium sp. that attack crops. This study aims to detect the biosurfactant activity of Bacillus sp. and its potential as an antifungal agent against the fungi Ganoderma boninense and Fusarium sp. Biosurfactants were produced in mineral salt medium (MSM) by harvesting cell-free supernatants. Screening of biosurfactant-producing isolates was carried out using an oil-spreading assay, a hemolysis assay, and an emulsification index. The antifungal activity of the isolates was then tested using the agar diffusion method. The LP04 isolate was closely related to Bacillus thuringiensis with a 99% similarity level. It has the potential to have biosurfactant activity, which is characterized by a positive result on the oil spreading assay test and has an emulsification index of 48.33±2.87%. The cell-free supernatants of the bacterial isolate were able to inhibit the growth of Ganoderma boninense and Fusarium sp. with growth inhibition rates of 51.11% and 56.92%, respectively.

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菌株 LP04 分离物的生物表面活性剂活性及其对骨灵芝和镰刀菌的抗真菌效力

生物表面活性剂是一类两性分子，各种微生物都能产生。生物表面活性剂被用作生物农药和生物防治剂，因为它们具有抗菌活性，特别是对多种真菌病原体，如侵袭农作物的灵芝和镰刀菌有抗真菌作用。本研究旨在检测芽孢杆菌的生物表面活性剂活性及其作为抗真菌剂对付灵芝和镰刀菌的潜力。利用展油试验、溶血试验和乳化指数对产生生物表面活性剂的分离物进行了筛选。然后用琼脂扩散法测试了分离物的抗真菌活性。LP04 分离物与苏云金芽孢杆菌关系密切，相似度达 99%。它具有潜在的生物表面活性剂活性，其特点是在铺油试验中呈阳性结果，乳化指数为 48.33±2.87%。该细菌分离物的无细胞上清液能够抑制骨灵芝和镰刀菌的生长，抑制率分别为 51.11% 和 56.92%。

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来源期刊

HAYATI Journal of Biosciences Agricultural and Biological Sciences-Agricultural and Biological Sciences (all)

CiteScore

1.10

自引率

0.00%

发文量

审稿时长

24 weeks

期刊介绍： HAYATI Journal of Biosciences (HAYATI J Biosci) is an international peer-reviewed and open access journal that publishes significant and important research from all area of biosciences fields such as biodiversity, biosystematics, ecology, physiology, behavior, genetics and biotechnology. All life forms, ranging from microbes, fungi, plants, animals, and human, including virus, are covered by HAYATI J Biosci. HAYATI J Biosci published by Department of Biology, Bogor Agricultural University, Indonesia and the Indonesian Society for Biology. We accept submission from all over the world. Our Editorial Board members are prominent and active international researchers in biosciences fields who ensure efficient, fair, and constructive peer-review process. All accepted articles will be published on payment of an article-processing charge, and will be freely available to all readers with worldwide visibility and coverage.