Salt and Heat Stress Trigger Morpho-Physiological Changes, Antioxidant Enzyme and Secondary Metabolites Gene Expression in Rice (Oryza sativa L.)

Q3 Agricultural and Biological Sciences

HAYATI Journal of Biosciences Pub Date : 2023-11-14 DOI:10.4308/hjb.31.2.256-270

Mohammad Ubaidillah, Noor Rozzita, Mitha Aprilia Mufadilah, Nurhaliza Thamrin, Agung Nugroho Puspito, Kyung Min Kim

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引用次数: 0

Abstract

Climate change significantly increases salt and heat stress in rice plants. This condition causes plants to activate antioxidant enzymes and produce secondary metabolites. This study aimed to determine the morpho-physiological changes and gene expression profiles of antioxidant enzymes and secondary metabolites. This study used a completely randomized design factorial. The first factor was local rice varieties (IR64, Silaun, and Cigeulis), and the second factor was stress treatments (control, NaCl 150 mM, 40°C, and NaCl 150 mM + 40°C). The results showed that multiple stress significantly affected the plant height, stem length, stem diameter, leaf area, root length, total main root, plant biomass, necrotic length, chlorophyll content, relative water content, and plant ROS production. Multiple stress could up-regulate the gene expression of antioxidant enzymes (Mn-SOD, Cu/Zn SOD, Cytosolic APX, OsAPX1, CAT, OsCATA, and GPOD) in rice after stress combination treatments and increase the secondary metabolites gene expression (P5CS and GABA-T) in all rice varieties. Still, the OsNOMT gene was only active in the Cigeulis variety.

查看原文本刊更多论文

盐胁迫和热胁迫引发水稻（Oryza sativa L.）的形态生理变化、抗氧化酶和次生代谢物基因表达

气候变化大大增加了水稻植物的盐胁迫和热胁迫。这种情况会导致植物激活抗氧化酶并产生次生代谢产物。本研究旨在确定抗氧化酶和次生代谢物的形态生理变化和基因表达谱。本研究采用完全随机设计因子法。第一个因子是当地水稻品种（IR64、Silaun 和 Cigeulis），第二个因子是胁迫处理（对照、NaCl 150 mM、40°C 和 NaCl 150 mM + 40°C）。结果表明，多重胁迫对植株高度、茎长、茎径、叶面积、根长、总主根、植株生物量、坏死长度、叶绿素含量、相对含水量和植株ROS产生量均有显著影响。多重胁迫综合处理后，水稻抗氧化酶（Mn-SOD、Cu/Zn SOD、细胞APX、OsAPX1、CAT、OsCATA和GPOD）基因表达上调，次生代谢物基因（P5CS和GABA-T）表达增加。不过，OsNOMT 基因只在 Cigeulis 品种中活跃。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

HAYATI Journal of Biosciences Agricultural and Biological Sciences-Agricultural and Biological Sciences (all)

CiteScore

1.10

自引率

0.00%

发文量

审稿时长

24 weeks

期刊介绍： HAYATI Journal of Biosciences (HAYATI J Biosci) is an international peer-reviewed and open access journal that publishes significant and important research from all area of biosciences fields such as biodiversity, biosystematics, ecology, physiology, behavior, genetics and biotechnology. All life forms, ranging from microbes, fungi, plants, animals, and human, including virus, are covered by HAYATI J Biosci. HAYATI J Biosci published by Department of Biology, Bogor Agricultural University, Indonesia and the Indonesian Society for Biology. We accept submission from all over the world. Our Editorial Board members are prominent and active international researchers in biosciences fields who ensure efficient, fair, and constructive peer-review process. All accepted articles will be published on payment of an article-processing charge, and will be freely available to all readers with worldwide visibility and coverage.