Phenotypic and Genotypic Antimicrobial Resistance Profile of Salmonella spp. Isolated from Kampung Chicken Carcasses

Q3 Agricultural and Biological Sciences

HAYATI Journal of Biosciences Pub Date : 2023-11-13 DOI:10.4308/hjb.31.2.229-240

S. Nurjanah, W. Rahayu, Stephen Sanjaya

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Abstract

Kampung chicken meats have been widely consumed in Indonesia as well as broiler chicken. However, the extensive rearing allowed multidrug-resistant (MDR) bacteria exposure to kampung chicken, including through horizontal gene transfer. This study aimed to observe the correlation between the phenotypic and genotypic antimicrobial resistance profile of Salmonella spp. isolated from kampung chicken carcasses. Phenotypic antimicrobial resistance was evaluated by Kirby-Bauer's disk diffusion method, while the detection of drug resistance genes in seventeen isolates of Salmonella was carried out by PCR. All (17/17) isolates were susceptible to ampicillin and chloramphenicol. Most isolates of Salmonella were resistant to erythromycin (82%; 14/17), while the decreased susceptibility (intermediate category) most occurred in oxytetracycline (82%; 14/17). Salmonella Typhimurium showed a resistance pattern to more antimicrobial groups than S. Newport and S. Weltevreden. Several antimicrobial resistance genes (blaTEM, tetG, cmlA, gyrA) were present in all (17/17) isolates of Salmonella spp. Resistance to antimicrobial agents and the presence of resistance genes were not always related. This study could provide beneficial information regarding the transmission of antimicrobial resistance among Salmonella spp. from kampung chickens.

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从甘榜鸡屠体中分离出的沙门氏菌属的表型和基因型抗菌药耐药性概况

印尼人广泛食用甘榜鸡肉和肉鸡。然而，广泛的饲养使耐多药（MDR）细菌暴露于甘榜鸡肉中，包括通过水平基因转移。本研究旨在观察从甘榜鸡尸体中分离出的沙门氏菌的表型和基因型抗菌谱之间的相关性。表型抗菌药耐药性通过柯比鲍尔盘扩散法进行评估，而 17 个沙门氏菌分离物的耐药基因则通过 PCR 进行检测。所有（17/17）分离菌株都对氨苄西林和氯霉素敏感。大多数沙门氏菌分离物对红霉素（82%；14/17）具有抗药性，而对土霉素（82%；14/17）的敏感性降低（中间类别）最多。与纽波特沙门氏菌和韦尔泰夫雷登沙门氏菌相比，鼠伤寒沙门氏菌对更多的抗菌药组表现出耐药模式。所有（17/17）分离的沙门氏菌中都存在几种抗菌药耐药基因（blaTEM、tetG、cmlA、gyrA）。对抗菌药的耐药性与耐药基因的存在并不总是相关的。这项研究可提供有关甘榜鸡沙门氏菌耐药性传播的有益信息。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

HAYATI Journal of Biosciences Agricultural and Biological Sciences-Agricultural and Biological Sciences (all)

CiteScore

1.10

自引率

0.00%

发文量

审稿时长

24 weeks

期刊介绍： HAYATI Journal of Biosciences (HAYATI J Biosci) is an international peer-reviewed and open access journal that publishes significant and important research from all area of biosciences fields such as biodiversity, biosystematics, ecology, physiology, behavior, genetics and biotechnology. All life forms, ranging from microbes, fungi, plants, animals, and human, including virus, are covered by HAYATI J Biosci. HAYATI J Biosci published by Department of Biology, Bogor Agricultural University, Indonesia and the Indonesian Society for Biology. We accept submission from all over the world. Our Editorial Board members are prominent and active international researchers in biosciences fields who ensure efficient, fair, and constructive peer-review process. All accepted articles will be published on payment of an article-processing charge, and will be freely available to all readers with worldwide visibility and coverage.