Hereditas最新文献

{"title":"Diagnostic value of LncRNA JPX in osteoporotic fracture (OPF) and its role in inhibiting osteogenic differentiation by targeting miR-219a-5p.","authors":"Junli Li, Yiwei Li, Ningning Zhang, Zhijian Cheng","doi":"10.1186/s41065-026-00685-8","DOIUrl":"https://doi.org/10.1186/s41065-026-00685-8","url":null,"abstract":"<p><strong>Background: </strong>This study was designed to investigate the diagnostic potential of the long non-coding RNA JPX in osteoporotic fractures (OPF) and to explore its functional role in the regulation of fracture healing.</p><p><strong>Methods: </strong>A total of 244 patients with osteoporosis were enrolled, including 110 with osteoporosis (OP) and 134 with OPF. Expression levels of JPX, miR-219a-5p, and osteogenic markers (OPG, ALP, Collagen I, OCN) were detected by RT-qPCR. ROC analysis was employed to assess the clinical value of JPX. In human bone marrow mesenchymal stem cells (hBMSCs), gain- and loss-of-function experiments were performed to modulate JPX and miR-219a-5p expression. Cell proliferation was evaluated by CCK-8 assay. Flow cytometry assessed apoptosis. Dual luciferase reporter assays and RIP experiments validated the targeting relationship between JPX and miR-219a-5p.</p><p><strong>Results: </strong>JPX was upregulated in the OPF group, with good diagnostic performance (AUC = 0.897; specificity = 76.87%; sensitivity = 84.55%), and JPX was identified an independent predictive factor. DFH and NFH could also be distinguished. JPX upregulation inhibited proliferation, promoted apoptosis, and suppressed osteogenic markers and ALP activity. JPX largely resides in the cytoplasm and directly binds miR-219a-5p, forming a ceRNA axis; upregulation of miR-219a-5p could reverse these effects.</p><p><strong>Conclusion: </strong>JPX is associated with fracture risk and delayed healing, potentially influencing osteogenesis by sponge-like regulation of miR-219a-5p.</p>","PeriodicalId":12862,"journal":{"name":"Hereditas","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147814219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"STEAP2-associated modulation of PI3K/AKT/mTOR signaling contributes to ginkgetin-induced apoptosis in bladder cancer cells.","authors":"Pengze Wu, Lin Chen, Jin Yang, Zhengkang Liang, Xiaofeng Yin, Shaowen Zhou, Zhilin Deng, Yafei Yang","doi":"10.1186/s41065-026-00686-7","DOIUrl":"https://doi.org/10.1186/s41065-026-00686-7","url":null,"abstract":"<p><strong>Background: </strong>Bladder cancer remains a major urologic malignancy with substantial recurrence and progression risk, underscoring the need for mechanism-informed therapeutic candidates. Ginkgetin, a biflavonoid derived from Ginkgo biloba leaves, has shown antitumor potential in several cancer settings, yet its key signaling axis and actionable molecular node in bladder cancer have not been systematically defined.</p><p><strong>Methods: </strong>We evaluated ginkgetin across multiple bladder cancer cell lines (5637, T24, HT-1376, J82) and normal urothelial cells (SV-HUC-1) using viability assays and IC₅₀ estimation. Antitumor phenotypes were assessed by colony formation, wound-healing migration assays, EMT marker profiling, and Annexin V/PI flow cytometry. Network pharmacology and RNA-seq were integrated to prioritize enriched pathways, followed by western blot validation of PI3K/AKT/mTOR phosphorylation. An insulin reactivation (\"rescue\") strategy was used to functionally test pathway dependence. Transcriptome-derived candidates were further examined by RT-qPCR and STEAP2 overexpression to probe node-level involvement. In addition, molecular docking and 100-ns molecular dynamics simulations were performed to characterize ligand-target binding stability.</p><p><strong>Results: </strong>Ginkgetin suppressed bladder cancer cell viability in a time- and dose-dependent manner at low micromolar concentrations, while normal urothelial cells required markedly higher exposures. Functionally, ginkgetin reduced clonogenic survival, inhibited migration, and shifted EMT features toward an epithelial phenotype. Apoptosis increased in parallel, accompanied by a pro-apoptotic protein signature. Multi-omics and network analyses converged on PI3K-Akt signaling, and experimental validation showed that ginkgetin primarily dampened pathway output by reducing PI3K/AKT/mTOR phosphorylation rather than total protein abundance. Insulin-mediated reactivation partially reversed phosphorylation suppression and attenuated apoptosis-related shifts, supporting a functional link between axis inactivation and apoptotic tendency. STEAP2 was consistently downregulated after treatment, and STEAP2 overexpression partially counteracted apoptosis-associated changes.</p><p><strong>Conclusion: </strong>These findings support a coherent \"phenotype-pathway-node\" model in which ginkgetin inhibits malignant phenotypes and promotes apoptosis in bladder cancer cells, associated with reduced PI3K/AKT/mTOR activity and STEAP2 downregulation. The PI3K/AKT/mTOR axis and STEAP2 emerge as testable mechanistic entry points for further translational validation.</p>","PeriodicalId":12862,"journal":{"name":"Hereditas","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147814228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and validation of biomarkers related to lysine β-hydroxybutyrylation in chronic obstructive pulmonary disease based on transcriptomics data.","authors":"Jing Lei, Jing Zhang, Linfei Yang, Yubao Chen","doi":"10.1186/s41065-026-00682-x","DOIUrl":"https://doi.org/10.1186/s41065-026-00682-x","url":null,"abstract":"","PeriodicalId":12862,"journal":{"name":"Hereditas","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2026-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147769857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"miR-1229-3p promotes epithelial-mesenchymal transition and metastasis of cervical cancer cells by targeting FBXL5.","authors":"Donglian Lan, Xiaojing Sun, Lei Yao, Bo Cao","doi":"10.1186/s41065-026-00679-6","DOIUrl":"https://doi.org/10.1186/s41065-026-00679-6","url":null,"abstract":"","PeriodicalId":12862,"journal":{"name":"Hereditas","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2026-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147769948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of biomarkers related to γ-aminobutyric acid-associated genes in membranous nephropathy based on transcriptome data and clinical experiments.","authors":"Jie Luo, Min Chen, Jing Li, Yue Qi, Tingyu Chen, Huibin Nie","doi":"10.1186/s41065-026-00681-y","DOIUrl":"https://doi.org/10.1186/s41065-026-00681-y","url":null,"abstract":"","PeriodicalId":12862,"journal":{"name":"Hereditas","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2026-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147769920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A comprehensive analysis of the Cullin family reveals that CUL5 and CUL7 promote colorectal cancer progression and serve as prognostic markers.","authors":"Noura Al-Dayan","doi":"10.1186/s41065-026-00677-8","DOIUrl":"https://doi.org/10.1186/s41065-026-00677-8","url":null,"abstract":"","PeriodicalId":12862,"journal":{"name":"Hereditas","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2026-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147769807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tussilagone mitigates sepsis-induced acute lung injury in mice by suppressing RIPK1 expression.","authors":"Jie Gu, Yachen Cai, Jiangwen Yin, Mengjie Zhang, Jun Chen, Hongjun Miao","doi":"10.1186/s41065-026-00678-7","DOIUrl":"https://doi.org/10.1186/s41065-026-00678-7","url":null,"abstract":"","PeriodicalId":12862,"journal":{"name":"Hereditas","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2026-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147769906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hub genes of neutrophil extracellular traps in abdominal aortic aneurysm: a bioinformatics analysis.","authors":"Jinxin Liu, Bixuan Yue, Zhiying Shen, Bo Yang, Yuxuan Ou, Xiaowu Wang","doi":"10.1186/s41065-026-00663-0","DOIUrl":"https://doi.org/10.1186/s41065-026-00663-0","url":null,"abstract":"","PeriodicalId":12862,"journal":{"name":"Hereditas","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2026-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147728811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel KCNK3 variant in a child with pulmonary arterial hypertension.","authors":"Yi-Ming Zheng, Jia-Qi Jiang, Xuan Li, Hong-Biao Huang, Wen-Yu Zhuo, Xuan Tang, Ying Liu, Hai-Tao Lv","doi":"10.1186/s41065-026-00680-z","DOIUrl":"https://doi.org/10.1186/s41065-026-00680-z","url":null,"abstract":"","PeriodicalId":12862,"journal":{"name":"Hereditas","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2026-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147716654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Whole-transcriptome analysis of Aortic Stenosis reveals dysregulated RNA networks, immune cell infiltration, and NADK2 as a candidate regulator.","authors":"Feng-Xia Wang, Ming-Jun Duan, Hao-Qiang Guo, Jia-Qing Yu, Fen Liu, Nilupaer Aisikeer, Yi-Tong Ma, Xiang Xie","doi":"10.1186/s41065-026-00675-w","DOIUrl":"https://doi.org/10.1186/s41065-026-00675-w","url":null,"abstract":"","PeriodicalId":12862,"journal":{"name":"Hereditas","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2026-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147716660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}