miR-370-3p通过靶向INO80影响绝经后骨质疏松症的进展。

IF 2.5 3区生物学

Hereditas Pub Date : 2025-07-22 DOI:10.1186/s41065-025-00502-8

Zhen Yang, Yuqi Sheng, Xiangjie Liu, Meini Cen, Yong Xu

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引用次数: 0

摘要

背景：绝经后骨质疏松症（PMO）是公认的骨质疏松症（OP）的主要类别。本研究旨在探讨miR-370-3p在PMO患者中的表达水平及其对绝经后妇女骨质疏松的预测作用，探讨miR-370-3p在PMO中的分子机制。方法：采用RT-qPCR检测miR-370-3p的表达。CCK-8检测MC3T3-E1细胞的增殖情况。流式细胞术检测细胞凋亡。通过双荧光素酶报告基因检测证实了miR-370-3p和INO80之间的直接相互作用。结果：发现miR-370-3p在骨质疏松患者中表达水平上调，miR-370-3p在调节成骨细胞增殖、凋亡和分化中发挥显著作用。此外，miR-370-3p靶向INO80，影响PMO的疾病进展。结论：miR-370-3p/INO80可能作为PMO诊断和治疗管理的有希望的生物标志物。

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miR-370-3p affects the progression of postmenopausal osteoporosis through targeting INO80.

Background: Postmenopausal osteoporosis (PMO) is acknowledged as a principal category of osteoporosis (OP). The aim of this study was to investigate the level of miR-370-3p in PMO patients and its predictive effect on osteoporosis in postmenopausal women, and to explore the molecular mechanism of miR-370-3p on PMO.

Methods: The expression of miR-370-3p was assessed using RT-qPCR. Cell proliferation of MC3T3-E1 cells was evaluated through CCK-8 assays. Cell apoptosis was detected by flow cytometry. The direct interaction between miR-370-3p and INO80 was confirmed via dual-luciferase reporter assays.

Results: The level of miR-370-3p was found to be upregulated in osteoporosis patients, and miR-370-3p played a significant role in regulating the proliferation, apoptosis and differentiation of osteoblasts. In addition, miR-370-3p targeted INO80 and affected the disease progression of PMO.

Conclusions: miR-370-3p/INO80 may serve as a promising biomarker for both the diagnosis and therapeutic management of PMO.

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来源期刊

Hereditas Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

3.80

自引率

3.70%

发文量

期刊介绍： For almost a century, Hereditas has published original cutting-edge research and reviews. As the Official journal of the Mendelian Society of Lund, the journal welcomes research from across all areas of genetics and genomics. Topics of interest include human and medical genetics, animal and plant genetics, microbial genetics, agriculture and bioinformatics.