The potential mechanism of Saikosaponin D against luminal A breast cancer based on bioinformatical analysis, molecular docking and in vitro studies.

IF 2.7 3区生物学

Hereditas Pub Date : 2025-07-24 DOI:10.1186/s41065-025-00510-8

Lichang Yang, Ru Chang, Jianzhen Pan, Shan Huang

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引用次数: 0

Abstract

Background: Saikosaponin D (SSD) has been shown to have the strongest anti-tumor activity. This study aimed to explore the effects and potential molecular mechanism of Saikosaponin D (SSD) against estrogen receptor-positive breast cancer.

Methods: MCF-7 and T-47D cell lines were treated with a series of concentrations of SSD. Growth, cell cycle distribution, and apoptosis tests were performed. Next, potential targets of SSD against breast cancer were predicted. The targets for SSD were collected from HERB database and PharmMapper Server and displayed accoding to degree.

Results: there was a dose-dependent decrease in MCF-7 and T-47D cancer cell viability and the the half maximal inhibitory concentrations were 7.31 ± 0.63 µM and 9.06 ± 0.45 µM, respectively. Treatment with SSD decreased cell proliferation, arrested cell cycle at G1, and induced cell apoptosis. There were 227 potential targets of SSD against breast cancer, among which ESR1 was a hub gene. SSD treatment can reduce the protein levels of estrogen receptor α (ERα), Cyclin D1 (CCND1), and the proto-oncogene c-Myc (c-Myc).

Conclusion: SSD may have therapeutic potential in estrogen receptor-positive breast cancer, may through its suppression on ESR1.

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基于生物信息学分析、分子对接和体外研究柴草皂苷D抗腔内A型乳腺癌的潜在机制。

背景：柴草皂苷D （Saikosaponin D， SSD）具有较强的抗肿瘤活性。本研究旨在探讨柴草皂苷D （Saikosaponin D， SSD）对雌激素受体阳性乳腺癌的作用及其可能的分子机制。方法：用不同浓度的SSD处理MCF-7和T-47D细胞。进行生长、细胞周期分布和细胞凋亡试验。接下来，我们预测了SSD治疗乳腺癌的潜在靶点。从HERB数据库和PharmMapper Server中收集SSD的目标，并按程度显示。结果：MCF-7和T-47D癌细胞活力呈剂量依赖性降低，最大抑制浓度的一半分别为7.31±0.63µM和9.06±0.45µM。SSD降低细胞增殖，阻滞G1期细胞周期，诱导细胞凋亡。SSD抗乳腺癌的潜在靶点有227个，其中ESR1为枢纽基因。SSD治疗可降低雌激素受体α (ERα)、细胞周期蛋白D1 （CCND1）和原癌基因c-Myc （c-Myc）的蛋白水平。结论：SSD可能通过抑制ESR1对雌激素受体阳性乳腺癌有治疗作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Hereditas Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

3.80

自引率

3.70%

发文量

期刊介绍： For almost a century, Hereditas has published original cutting-edge research and reviews. As the Official journal of the Mendelian Society of Lund, the journal welcomes research from across all areas of genetics and genomics. Topics of interest include human and medical genetics, animal and plant genetics, microbial genetics, agriculture and bioinformatics.