Graefe’s Archive for Clinical and Experimental Ophthalmology最新文献

{"title":"One year follow up of descemet stripping only: corneal tomography changes and visual acuity outcomes.","authors":"Alberto Villarrubia Cuadrado, Álvaro Sánchez-Ventosa, Timoteo González-Cruces, Vanessa Díaz-Mesa, Marta Villalva-González, Elisa Palacín Miranda, María Dolores López Pérez, Javier Gersol Pérez-Angulo, José Carlos Díaz-Ramos, Antonio Cano-Ortiz","doi":"10.1007/s00417-025-06747-3","DOIUrl":"https://doi.org/10.1007/s00417-025-06747-3","url":null,"abstract":"<p><strong>Background: </strong>Descemet Stripping Only (DSO) is a minimally invasive procedure for Fuchs' Endothelial Corneal Dystrophy (FECD) that leverages peripheral endothelial regeneration without donor tissue. This study evaluates visual and anatomical outcomes of DSO and the role of guttae distribution in recovery.</p><p><strong>Methods: </strong>A prospective study included 30 eyes of FECD patients undergoing DSO. Patients were classified by guttae distribution: type A (central) or type B (0º-180º meridian). Preoperative and postoperative outcomes, including corrected distance visual acuity (CDVA), central corneal thickness (CCT), and endothelial cell density (ECD), were assessed over 12 months.</p><p><strong>Results: </strong>Of 26 patients completing follow-up, 42.31% achieved a CDVA ≥ 0.22 logMAR by week 4, and 84.62% by month 12. Type B patients recovered faster, reaching CDVA ≥ 0.10 logMAR in 56 days versus 112 days for type A. Mean CCT improved from 606.96 ± 60.97 μm to 550.40 ± 29.67 μm (p = 0.0001). Postoperative ECD averaged 1402.7 ± 130.56 cells/mm². Corneal densitometry improved significantly but remained higher than controls.</p><p><strong>Conclusions: </strong>DSO is an effective treatment for FECD with adequate peripheral endothelial reserve. Guttae distribution affects recovery speed, but final outcomes are similar. If no improvement occurs by the third month, a rescue DMEK is effective. Keratometry remains unchanged, epithelial thickness slightly increases, and corneal densitometry improves postoperatively.</p><p><strong>Key messages: </strong>What is know: DSO is a donor-free surgical option for endothelial dystrophies like Fuchs' dystrophy, utilizing the eye's natural endothelial regenerative capacity in selected patients. Success relies on residual endothelial cell density and biomarkers like preoperative corneal thickness to predict regeneration outcomes.</p><p><strong>What is new: </strong>Pattern of guttae distribution influences recovery timeline in DSO: Patients with type B guttae distribution (0º-180º meridian) achieve a CDVA of 0.1 logMAR faster than those with type A (central guttae), though finalvisual acuity, pachymetry, and endothelial cell density are similar at 12 months. DSO as an effective treatment for fuchs' endothelial corneal dystrophy: Descemet stripping only achieves corneal clearance in most cases within 3-4 months, with significant CDVA and quality of life improvements (NEI VFQ-25 scores), supporting its role as a minimally invasive alternative to donor graft surgeries.</p>","PeriodicalId":12795,"journal":{"name":"Graefe’s Archive for Clinical and Experimental Ophthalmology","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144014243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Peripheral retinal and choroidal thickness of eyes with reticular pseudodrusen.","authors":"Je Moon Yoon, Hoon Noh, Seung Wan Nam, Don-Il Ham","doi":"10.1007/s00417-025-06842-5","DOIUrl":"https://doi.org/10.1007/s00417-025-06842-5","url":null,"abstract":"<p><strong>Purpose: </strong>This study aimed to investigate the peripheral retinal and choroidal thicknesses in eyes with reticular pseudodrusen (RPD).</p><p><strong>Methods: </strong>Imaging data of ultrawide-field swept-source optical coherence tomography taken from 40 patients with age-related macular degeneration (AMD) were analyzed. Thirty-nine eyes of 20 patients had RPD (RPD group) and 39 eyes of 20 patients had no RPD (control group). Eyes with RPD were divided into localized/intermediate and diffuse types according to the fundus distribution. The retinal and choroidal thicknesses were measured at the fovea and six peripheral points in each eye, and the results were statistically analyzed.</p><p><strong>Results: </strong>The mean ages were 78.0 ± 6.1 years in the RPD group and 74.4 ± 7.2 years in the control group. The RPD group showed a thinner retina only at the nasal point (p = 0.002) than the control group. The choroid was significantly thinner in the RPD group than in the control group at most peripheral points, except for the far temporal and far inferior points. Diffuse and localized/intermediate types of RPD eyes showed no significant difference in choroidal thickness, except at the nasal point, which was thinner in the diffuse type (p = 0.049).</p><p><strong>Conclusion: </strong>In eyes with RPD, most peripheral retinal thicknesses were not different from those of eyes with AMD without RPD; however, most peripheral choroidal thicknesses were thinner regardless of the fundus distribution status of the RPD. Nasal choroidal thickness may be associated with RPD severity.</p>","PeriodicalId":12795,"journal":{"name":"Graefe’s Archive for Clinical and Experimental Ophthalmology","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143985387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vitrectomy with macular peeling in eyes with vitreomacular interface disorders and nonexudative age-related macular degeneration.","authors":"Marco Pellegrini, Ginevra Giovanna Adamo, Chiara Vivarelli, Laura Sarti, Pietro Maria Talli, Francesco Nasini, Francesco Parmeggiani, Marco Mura","doi":"10.1007/s00417-025-06848-z","DOIUrl":"https://doi.org/10.1007/s00417-025-06848-z","url":null,"abstract":"<p><strong>Background: </strong>The aim of the study was to assess the outcomes of pars plana vitrectomy and macular peeling for vitreomacular interface disorders in eyes with coexisting nonexudative age-related macular degeneration (AMD), and to compare the rate of AMD progression in operated and fellow eyes.</p><p><strong>Methods: </strong>This retrospective comparative study included patients with bilateral nonexudative AMD and unilateral vitreomacular interface disorder who underwent pars plana vitrectomy with internal limiting membrane peeling. Controlateral unoperated eyes were used as a control group. Cox proportional hazards regression analysis was used to compare the incidence of macular neovascularization, geographic atrophy and progression to late AMD in operated eyes and fellow eyes.</p><p><strong>Results: </strong>142 eyes of 71 patients were included. The mean follow-up duration was 17.5 ± 15.5 months. Best corrected visual acuity significantly improved in operated eyes (from 0.47 ± 0.25 to 0.21 ± 0.18 logMAR; p < 0.001), while no significant difference was observed in fellow eyes (from 0.18 ± 0.22 to 0.33 ± 0.56 logMAR; p = 0.055). Central retinal thickness improved in operated eyes (from 0402.9 ± 79.9 to 296.1 ± 48.4 µm; p < 0.001), while no change in fellow eyes was observed (from 296.1 ± 48.4 to 312.7 ± 110.6 µm; p = 0.205). Vitrectomy was not associated with the risk of developing macular neovascularization (hazard ratio [HR] = 0.30; 95% confidence intervals (CI) = 0.08-1.09; p = 0.068); geographic atrophy (HR = 1.01, 95% CI = 0.32-3.12; p = 0.990) nor progression to late AMD (HR = 0.64, 95% CI = 0.28-1.49; p = 0.307).</p><p><strong>Conclusions: </strong>Pars plana vitrectomy with macular peeling for vitreomacular interface disorders in eyes with coexisting nonexudative AMD is associated with positive visual and functional outcomes, with no shot-term increased risk of AMD progression.</p>","PeriodicalId":12795,"journal":{"name":"Graefe’s Archive for Clinical and Experimental Ophthalmology","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144004056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Changes in lower eyelid positions after Müller's muscle-conjunctival resection with tarsectomy for the correction of upper eyelid ptosis.","authors":"Le-Yu Chen, Shu-Lang Liao, Yi-Hsuan Wei","doi":"10.1007/s00417-025-06829-2","DOIUrl":"https://doi.org/10.1007/s00417-025-06829-2","url":null,"abstract":"<p><strong>Purpose: </strong>This study aims to evaluate the effect of Müller's muscle-conjunctival resection (MMCR) with tarsectomy on lower eyelid position in patients with unilateral aponeurotic ptosis in the East Asian population.</p><p><strong>Methods: </strong>A retrospective review was conducted on patients undergoing unilateral MMCR surgery. Margin reflex distance (MRD) 1 and 2 were measured preoperatively and three months postoperatively. The primary outcome was the change in MRD2 following surgery. Additionally, we investigated the relationship between the relative change of MRD1 and that of MRD2. The secondary outcome was the influence of factors including age, sex, and preoperative MRD on the change in MRD2.</p><p><strong>Results: </strong>Sixty-one patients were included. In the ptotic eye, postoperative MRD1 increased from 1.47 ± 0.88 mm to 3.57 ± 1.00 mm (p < 0.001), while MRD2 decreased from 5.53 ± 0.97 mm to 5.31 ± 0.96 mm (p = 0.007). A negative correlation was observed between the relative change of MRD1 and MRD2 in the ptotic eye (r = -0.335, unstandardized coefficient [B] = -0.016, 95% confidence interval [CI] -0.028 to -0.004, p = 0.009). Additionally, a positive correlation was found between the relative change in MRD2 in the ptotic and normal eyes (r = 0.818, B = 0.760, 95% CI 0.621 to 0.899, p < 0.001). Preoperative MRD2 in the ptotic eye was a predictor of postoperative MRD2 reduction (B = -0.189, 95% CI -0.327 to -0.051, p = 0.008).</p><p><strong>Conclusion: </strong>This study demonstrates that MMCR alters lower eyelid position, with reductions in MRD2 correlating with the degree of upper eyelid correction. It is important to inform patients of this potential alteration preoperatively.</p>","PeriodicalId":12795,"journal":{"name":"Graefe’s Archive for Clinical and Experimental Ophthalmology","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144019564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of intraocular pressure during phacoemulsification in patients with either diabetic retinopathy or glaucoma; a randomized controlled feasibility trial.","authors":"Raffaele Raimondi, Karmen Sow, Tunde Peto, Nicholas Wride, Maged S Habib, Alan Sproule, Alyson K Muldrew, Michael Quinn, David H Steel","doi":"10.1007/s00417-025-06839-0","DOIUrl":"https://doi.org/10.1007/s00417-025-06839-0","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Purpose: &lt;/strong&gt;To investigate whether performing phacoemulsification with a lower infusion pressure using the Centurion active sentry system affects surgical efficiency, complications and a range of clinical and imaging parameters compared to the higher pressures routinely used in patients with cataract and concomitant diabetic retinopathy and glaucoma.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Setting: &lt;/strong&gt;Sunderland Eye Infirmary, Sunderland, United Kingdom.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Design: &lt;/strong&gt;Masked observer randomized controlled feasibility trial.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;Patients with cataracts undergoing routine phacoemulsification with either diabetic retinopathy or primary open-angle glaucoma of any severity were included and randomized to an infusion pressure of 30 ('LOW') or 60 ('HIGH') mmHg. All other fluidic settings were standardized. Surgical metrics and a range of imaging and clinical variables were measured pre- and postoperatively on days 1, 21 and 40.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;Seventy eyes from 70 patients underwent surgery and completed follow-up. Forty-one patients had diabetic retinopathy and 29 had glaucoma. There was no difference in any of the recorded surgical metrics including cumulative dissipated energy (CDE) between the two randomization groups (mean CDE 6.5 versus 6.1 percent seconds in the HIGH and LOW groups respectively, p = 0.68). There were no patients in either group with posterior capsule rupture or other intraoperative complications. There was no significant difference in the number of patients with raised intraocular pressure (IOP) on day 1. Seven (21.2%) patients in the LOW and 5 (13.3%) in the HIGH group had slit lamp detectable corneal oedema on day 1, which had all resolved by day 21. There were no between group differences for visual acuity, IOP, corneal thickness, and any of the optical coherence tomography (OCT) acquired measures at any of the time points. The foveal avascular zone perimeter and area were significantly smaller on day 21 than at baseline in the HIGH group as compared to the LOW group (P = 0.03 and 0.04 respectively), with a corresponding increase in the superficial vascular plexus density (p = 0.04).&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Conclusion: &lt;/strong&gt;Using an infusion pressure of 30mmHg with standardized aspiration fluidic settings on the Centurion active sentry system did not decrease surgical efficiency or increase complication rates compared to a pressure of 60mmHg. The lower infusion pressure may cause fewer short-term changes in the retinal microvasculature, the long-term significance of which is unknown.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Key messages: &lt;/strong&gt;What is known Traditionally, phacoemulsification has been carried out under relatively high intraocular pressure (IOP) to mitigate the effects of post occlusion aspiration surge during lens removal.  A new enhanced phacoemulsification fluidics system has reduced surge allowing surgeons to operate at considerably lower, and more physiological IOP levels. What i","PeriodicalId":12795,"journal":{"name":"Graefe’s Archive for Clinical and Experimental Ophthalmology","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143997762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Refractive changes after cataract removal in infancy: comparing eyes with and without persistent fetal vasculature.","authors":"Ana Navarrete, Brice Nguedia Vofo, Hadas Mechoulam, Milka Matanis-Suidan, Nur Azem, Ilana Karshai, Ran David, Irene Anteby","doi":"10.1007/s00417-025-06841-6","DOIUrl":"https://doi.org/10.1007/s00417-025-06841-6","url":null,"abstract":"<p><strong>Purpose: </strong>To evaluate the refractive changes after congenital cataract surgery in persistent fetal vasculature (PFV) vs. non-PFV eyes.</p><p><strong>Methods: </strong>Retrospective study of 75 eyes with PFV or non-PFV congenital cataract, who underwent surgery before age 7 months (unilateral/first operated eye), during 2007-2018 at a tertiary referral center, with follow-up ≥ one-year.</p><p><strong>Results: </strong>27 eyes (36%) had PFV, 48 were non-PFV cataracts. Mean age (± SD) at surgery in children with PFV was 2.24 ± 1.23 months and 2.44 ± 1.51 months in the non-PFV group. Mean post-operative follow-up was 64.94 ± 34.67 months. 60% of the eyes remained aphakic for the entire follow-up. In aphakic children, the mean post-operative spherical equivalent (SE) in the PFV eye was + 18.74D, + 15.73D, + 13.88D, + 12.51D, + 11.29D at one-month, one-year, two-years, three-years and five-years respectively. In the non-PFV eye the SE was + 23.00D, + 20.44D, + 17.84D, + 17.52D, + 18.48D at one-month, one-year, two-years, three-years and five-years respectively. During the entire post-operative course, the SE remained less hyperopic in the PFV eyes (p < 0.01). The rate of emmetropization was similar for PFV and non-PFV eyes. Five-years after surgery the mean myopic shift was -6.82 ± 4.32 D in the PFV eyes and -5.47 ± 2.44D in the non-PFV eyes (p > 0.05). The changes in refraction error did not correlate with either presence of glaucoma, secondary cataract, amblyopia or strabismus.</p><p><strong>Conclusion: </strong>Eyes with PFV have a similar rate of emmetropization as other pediatric congenital cataracts. Interestingly, aphakic PFV eyes have a less hyperopic refraction during one-month and up to five-years after surgery as compared to non-PFV eyes.</p>","PeriodicalId":12795,"journal":{"name":"Graefe’s Archive for Clinical and Experimental Ophthalmology","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144005432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intracellular dark endothelial spots: A new imaging biomarker for the development of bullous keratopathy after cataract surgery.","authors":"Taiyo Shijo, Yukari Yagi-Yaguchi, Osama Ibrahim, Hirotsugu Kasamatsu, Yurina Mori-Ogiwara, Daisuke Tomida, Hisashi Noma, Dogru Murat, Takefumi Yamaguchi","doi":"10.1007/s00417-025-06846-1","DOIUrl":"https://doi.org/10.1007/s00417-025-06846-1","url":null,"abstract":"<p><strong>Purpose: </strong>Intracellular dark endothelial spots (IDES) on specular microscopy indicate corneal endothelial stress, and can be an imaging biomarker for subsequent endothelial cell density (ECD) loss after endothelial keratoplasty. This study evaluated the risk factors for the development of bullous keratopathy (BK) by comparing low ECD patients with or without IDES before cataract surgery.</p><p><strong>Methods: </strong>This retrospective study included 106 consecutive patients who underwent cataract surgery with a preoperative ECD of less than 1000 cells/mm². The patients were divided into 2 groups according to presence of IDES. The medical charts were retrospectively reviewed for preoperative central corneal thickness (CCT), ECD, anterior chamber depth (ACD), presence of IDES, presence of guttae, grade of nuclear sclerosis, intraoperative posterior capsule rupture (PCR) and development of BK after cataract surgery. The clinical factors were compared in patients with or without IDES and the risk factors for BK after cataract surgery were evaluated.</p><p><strong>Results: </strong>The mean preoperative ECD was 687 ± 124 cells/mm². IDES were observed in 41 eyes (38.7%). IDES were significantly more common in eyes with shallow ACD than in those with deep ACD (P = 0.033). Twenty-two eyes (20.8%) developed BK after cataract surgery. IDES were significantly associated with the development of BK (31.7%) compared to those without BK (13.9%, P = 0.03). CCT, nuclear sclerosis, PCR, and IDES were identified as significant risk factors for postoperative BK (P < 0.0083).</p><p><strong>Conclusion: </strong>IDES can be used as a new imaging biomarker that suggests a potential risk for development of BK in patients with shallow anterior chamber who are to undergo cataract surgery.</p>","PeriodicalId":12795,"journal":{"name":"Graefe’s Archive for Clinical and Experimental Ophthalmology","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143993572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gene therapy in neovascular age related macular degeneration: an update.","authors":"Erika Quesada, Sofía Rojas, Xiomara Campos, Lihteh Wu","doi":"10.1007/s00417-025-06837-2","DOIUrl":"https://doi.org/10.1007/s00417-025-06837-2","url":null,"abstract":"<p><p>Neovascular age-related macular degeneration (NV-AMD) is a leading cause of preventable blindness in the elderly. Intravitreal injections of anti-VEGF agents are currently the treatment of choice for NV-AMD. However this treatment is burdensome and fosters non-compliance which leads to inferior visual outcomes. Gene therapy has emerged as a promising therapeutic option for NV-AMD that may improve these outcomes. Potential risks of gene therapy include a potential immune response that may be elicited by the vector, accidental activation of oncogenes or inactivation of tumor suppresor genes leading to malignant transformation via insertational mutagenesis and integration of the viral DNA inserts into the host's DNA. The main strategy of current gene therapy for NV-AMD has focused on delivering transgenes that express anti-angiogenic proteins that directly or indirectly inhibit the VEGF pathway. Ixoberogene soroparvovec, RGX-314 and 4D-150 are the leading NV-AMD genetic treatment programs. Pre-clinical models suggest that genome surgery with clustered regularly interspaced short palindromic repeats (CRISPR) may be another option in the future.</p>","PeriodicalId":12795,"journal":{"name":"Graefe’s Archive for Clinical and Experimental Ophthalmology","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144011687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Neuroadaptation to multifocal intraocular lenses: preliminary observations of a cognitive influence.","authors":"Victoria S Pelak, Michael Taravella, Nathan C Grove, Jennifer L Patnaik","doi":"10.1007/s00417-025-06832-7","DOIUrl":"https://doi.org/10.1007/s00417-025-06832-7","url":null,"abstract":"","PeriodicalId":12795,"journal":{"name":"Graefe’s Archive for Clinical and Experimental Ophthalmology","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143994336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of ocular surface microbiota in children with blepharoconjunctivitis.","authors":"Burçin Çakır, Büşra Güner Sönmezoğlu, Elif Özözen Şahin, Mehmet Köroğlu, Nilgün Özkan Aksoy","doi":"10.1007/s00417-025-06836-3","DOIUrl":"https://doi.org/10.1007/s00417-025-06836-3","url":null,"abstract":"<p><strong>Purpose: </strong>To investigate the conjunctival and eyelid margin bacterial microbiota in children with blepharoconjunctivitis by using 16S rDNA amplicon sequencing.</p><p><strong>Methods: </strong>In this prospective cross-sectional study, 20 children aged between 3-15 years with blepharoconjunctivitis or blepharokeratokonjunctivitis formed Blepharitis Group and 21 children aged between 3-15 years without any ocular and sysemic diseases except mild refractive errors formed Control Group. Swap samples from all children were taken. The alpha diversity of the ocular surface microbiota within each group were evaluated by using Shannon's, Simpson, and Chao index. Beta diversity was evaluated by Bray Curtis index.</p><p><strong>Results: </strong>Microbiological diversity was higher in the patient group than in the control group. According to Shannon's, Simpson, and Chao index, there were statistically difference between groups (p: 0.000013, p:000003 p: 0.00235, respectively). According to the Bray Curtis index, the healthy eye microbiome in the control group is observed to be highly similar, consistent with other analyses, and the overlapping cluster with the blepharitis eye microbiome is quite low (pco1: 40.93%). Sphingoblump, Micrococus, Lacnospiracebacterium, Stenothermophilus, Aurelmonass, Micrococus, Blatiabeum, Delfiacdiovorans and Vellonella densities were found to be higher in the patient group.</p><p><strong>Conclusion: </strong>Both alpha and beta diversity analyses were significantly higher in pediatric age group patients with blepharitis. In addition, Lacnospiracebacterium, Stenothermophilus, Aurelmonass, Micrococus, Blatiabeum, Delfiacdiovorans and Vellonella densities were found to be higher, which may lead to future studies focused on diagnosis and treatment.</p>","PeriodicalId":12795,"journal":{"name":"Graefe’s Archive for Clinical and Experimental Ophthalmology","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143994128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}