Frontiers in Veterinary Science最新文献

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Hematologic follow-up of metamizole-associated Heinz body anemia in dogs. 犬甲硝唑相关性亨氏体贫血的血液学随访。
IF 3.1 2区 农林科学
Frontiers in Veterinary Science Pub Date : 2026-09-28 eCollection Date: 2026-01-01 DOI: 10.3389/fvets.2026.1913770
Cathrina Richter, Vera Geisen, Jelena Palić, René Dörfelt
{"title":"Hematologic follow-up of metamizole-associated Heinz body anemia in dogs.","authors":"Cathrina Richter, Vera Geisen, Jelena Palić, René Dörfelt","doi":"10.3389/fvets.2026.1913770","DOIUrl":"https://doi.org/10.3389/fvets.2026.1913770","url":null,"abstract":"<p><strong>Introduction: </strong>Heinz body (HB) anemia is uncommon in dogs. Metamizole is described as a potential trigger in isolated cases.</p><p><strong>Aim of the study: </strong>The present study describes the course of metamizole-associated HB anemia in dogs with the development of hematocrit, HBs, and eccentrocytes. In addition, the correlation between HBs, eccentrocytes, hematocrit, and reticulocytes is investigated.</p><p><strong>Materials and methods: </strong>In this prospective observational study, 25 dogs with metamizole-associated HB anemia were included. Hematologic examinations and blood smear evaluations for quantification of HBs and eccentrocytes (stained with brilliant cresyl blue and modified Wright's stain) were performed on day 0 and at the control intervals 1-3, 4-6, 7-13, and 14-20 days after discontinuation of metamizole and initiation of antioxidant treatment. Known alternative causes of oxidative erythrocyte damage were excluded. Data were analyzed using Kruskal-Wallis test, Friedman test, and Spearman correlation.</p><p><strong>Results: </strong>HB anemia was detected a median of 10 days after initiation of metamizole (median cumulative dose 720 mg/kg). On day 0, HB percentage did not correlate with metamizole dose or hematocrit. The eccentrocyte count did not correlate with metamizole dose, hematocrit, or reticulocyte count. However, a moderate to strong correlation was identified between HB percentage and reticulocyte count (<i>r</i> = 0.630). Median HBs and eccentrocytes decreased from 26.5% and 2.6% on day 0 to 1.3% and 0% on days 14-20, while median hematocrit increased from 24 to 33%. Four dogs received blood transfusions. All patients had at least one concurrent disease. Twenty-one dogs survived and four dogs were euthanized or died.</p><p><strong>Conclusion: </strong>In dogs with concurrent disease, metamizole administration may be associated with clinically relevant erythrocyte oxidative damage. Discontinuation of metamizole and initiation of antioxidant therapy were followed by a decrease in HBs and recovery of hematocrit. The use of metamizole in multimorbid dogs should be carefully considered and accompanied by regular hematology controls.</p>","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":"13 ","pages":"1913770"},"PeriodicalIF":3.1,"publicationDate":"2026-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13656853/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149181368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Bleomycin-based electrochemotherapy for recurrent oral CD8-positive epitheliotropic T-cell lymphoma in a dog with durable local control and necrotic regional lymph node changes: a case report. 博来霉素为基础的电疗治疗复发性口腔cd8阳性上皮性t细胞淋巴瘤,伴有持久的局部控制和坏死的区域淋巴结改变:1例报告。
IF 3.1 2区 农林科学
Frontiers in Veterinary Science Pub Date : 2026-09-28 eCollection Date: 2026-01-01 DOI: 10.3389/fvets.2026.1942467
Makoto Akiyoshi, Masaharu Hisasue
{"title":"Bleomycin-based electrochemotherapy for recurrent oral CD8-positive epitheliotropic T-cell lymphoma in a dog with durable local control and necrotic regional lymph node changes: a case report.","authors":"Makoto Akiyoshi, Masaharu Hisasue","doi":"10.3389/fvets.2026.1942467","DOIUrl":"https://doi.org/10.3389/fvets.2026.1942467","url":null,"abstract":"<p><strong>Background: </strong>Oral epitheliotropic T-cell lymphoma (ETCL) is an uncommon form of canine cutaneous lymphoma involving the oral mucosa and mucocutaneous junctions. Information regarding optimal treatment strategies and prognosis is limited. Electrochemotherapy (ECT) has demonstrated efficacy against various cutaneous and subcutaneous tumors in dogs and cats; however, reports describing its use for canine oral ETCL are scarce.</p><p><strong>Case description: </strong>A 13-year-old male Beagle presented with multiple nodular lesions involving the right maxillary gingiva, buccal mucosa, and mandibular gingiva. Histopathology and immunohistochemistry confirmed CD8-positive epitheliotropic T-cell lymphoma, with more than 90% of neoplastic cells expressing CD3 and CD8. Initial treatment with oclacitinib resulted in a reduction in the size and number of the oral lesions; however, disease progression was observed after 149 days. Bleomycin-based electrochemotherapy (BLM-ECT) was subsequently performed using intravenous bleomycin (20 mg/m<sup>2</sup>) followed by electric pulse delivery with a four-needle electrode (1,000 V/cm, eight pulses). Two BLM-ECT sessions were administered on days 205 and 238, resulting in marked reduction in the size and number of the oral lesions and prolonged local disease control. On day 354, enlargement of the left mandibular lymph node was observed. Histopathological examination revealed extensive necrosis that precluded definitive determination of lymphoma involvement. Although residual CD3-positive lymphocytes were identified, neither flow cytometry nor polymerase chain reaction for antigen receptor rearrangement (PARR) confirmed a neoplastic lymphoid population. A third BLM-ECT session was performed on day 555 for recurrent erythematous lesions of the median upper and lower lip mucosa, in which T-cell lymphoma was histologically confirmed. At the latest evaluation on day 608, no grossly visible oral lesions were detected, and there was no clinical evidence of systemic dissemination. The dog remained clinically well.</p><p><strong>Conclusion: </strong>BLM-ECT provided prolonged local disease control in a dog with recurrent multifocal oral CD8-positive ETCL that had progressed despite oclacitinib treatment. Unusual necrotic changes also developed in regional lymph nodes. The etiology of these changes remained undetermined, and a causal relationship with ECT could not be established. Investigation of pathological and immunological changes following ECT is warranted. BLM-ECT may represent a feasible and repeatable local treatment option for selected dogs with oral ETCL.</p>","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":"13 ","pages":"1942467"},"PeriodicalIF":3.1,"publicationDate":"2026-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13656399/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149181138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Correction: Statistical shape modeling of the geometric morphology of the canine femur, tibia, and patella. 修正:犬股骨、胫骨和髌骨几何形态的统计形状建模。
IF 3.1 2区 农林科学
Frontiers in Veterinary Science Pub Date : 2026-09-28 eCollection Date: 2026-01-01 DOI: 10.3389/fvets.2026.2000605
Jeremy Huart, Antonio Pozzi, Jason Bleedorn, Tung-Wu Lu, Sebastian Knell, Brian Park
{"title":"Correction: Statistical shape modeling of the geometric morphology of the canine femur, tibia, and patella.","authors":"Jeremy Huart, Antonio Pozzi, Jason Bleedorn, Tung-Wu Lu, Sebastian Knell, Brian Park","doi":"10.3389/fvets.2026.2000605","DOIUrl":"https://doi.org/10.3389/fvets.2026.2000605","url":null,"abstract":"<p><p>[This corrects the article DOI: 10.3389/fvets.2024.1366827.].</p>","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":"13 ","pages":"2000605"},"PeriodicalIF":3.1,"publicationDate":"2026-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13656843/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149181225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effects of quercetin supplementation on the quality and metabolomic characteristics of sex-sorted frozen semen from Australian Wagyu bulls. 添加槲皮素对澳大利亚和牛冷冻精液质量和代谢组学特性的影响。
IF 3.1 2区 农林科学
Frontiers in Veterinary Science Pub Date : 2026-09-28 eCollection Date: 2026-01-01 DOI: 10.3389/fvets.2026.1951637
Chao Yuan, Yuefu Yin, Xin Wang, Bo Yu, Junda Wu, Yuanfeng Zhao, Jing Liu, Kaikai Zhang, Wenzhang Zhou, Yongcai Zhu, Longxin Xu
{"title":"Effects of quercetin supplementation on the quality and metabolomic characteristics of sex-sorted frozen semen from Australian Wagyu bulls.","authors":"Chao Yuan, Yuefu Yin, Xin Wang, Bo Yu, Junda Wu, Yuanfeng Zhao, Jing Liu, Kaikai Zhang, Wenzhang Zhou, Yongcai Zhu, Longxin Xu","doi":"10.3389/fvets.2026.1951637","DOIUrl":"https://doi.org/10.3389/fvets.2026.1951637","url":null,"abstract":"<p><p>This study aimed to evaluate the effects of different concentrations of quercetin on the post-thaw sperm quality of sex-sorted frozen semen from Australian Wagyu bulls and to investigate the underlying metabolic regulatory mechanisms using untargeted metabolomics. Sex-sorted frozen semen from Australian Wagyu bulls was treated with 0, 10, 50, 100, 150, and 200 μM quercetin. Sperm motility parameters, plasma membrane integrity, acrosome integrity, mitochondrial membrane potential, DNA integrity, reactive oxygen species (ROS) levels, and antioxidant-related indices were assessed. Metabolomic profiling was further performed to compare the 50 μM quercetin-treated group with the control group. The results showed that the 50 μM quercetin group exhibited better sperm viability, motility, straight-line velocity (VSL), curvilinear velocity (VCL), and average path velocity (VAP), together with reduced ROS levels and increased activities of antioxidant enzymes, including SOD, CAT, and GSH-Px. High concentrations of quercetin (≥150 μM) adversely affected sperm motility and several functional parameters. Metabolomic analysis revealed clear metabolic differences between the 50 μM quercetin-treated group and the control group, with 131 upregulated and 395 downregulated differential metabolites identified. KEGG enrichment analysis indicated that these differential metabolites were mainly involved in lipid-related pathways, including glycerophospholipid metabolism and arachidonic acid metabolism. These results suggest that an appropriate concentration of quercetin may improve the post-thaw quality of sex-sorted frozen semen from Australian Wagyu bulls by alleviating oxidative stress and regulating lipid metabolism. This study provides experimental evidence and metabolomic insights for optimizing cryoprotectant formulations for bovine sex-sorted frozen semen.</p>","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":"13 ","pages":"1951637"},"PeriodicalIF":3.1,"publicationDate":"2026-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13656795/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149181242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Long-term CT and US imaging of canine prostatic carcinoma after prostatic artery embolization: a 900-day follow-up case report. 前列腺动脉栓塞后犬前列腺癌的长期CT和US成像:900天随访病例报告。
IF 3.1 2区 农林科学
Frontiers in Veterinary Science Pub Date : 2026-09-28 eCollection Date: 2026-01-01 DOI: 10.3389/fvets.2026.1947473
Saneyuki Isshiki, Sachiyo Tsuji-Kawahara, Mayu Ogata, Shinya Hisamoto, Moe Hashimoto, Tetsuya Hasegawa, Ikuo Tsunoda
{"title":"Long-term CT and US imaging of canine prostatic carcinoma after prostatic artery embolization: a 900-day follow-up case report.","authors":"Saneyuki Isshiki, Sachiyo Tsuji-Kawahara, Mayu Ogata, Shinya Hisamoto, Moe Hashimoto, Tetsuya Hasegawa, Ikuo Tsunoda","doi":"10.3389/fvets.2026.1947473","DOIUrl":"https://doi.org/10.3389/fvets.2026.1947473","url":null,"abstract":"<p><p>Canine prostatic carcinoma is a highly aggressive malignancy with a poor prognosis and limited long-term treatment options. Prostatic artery embolization (PAE) has emerged as a minimally invasive alternative, but long-term intraprostatic imaging changes after PAE in dogs remain poorly documented. We performed PAE on a 13-year-old neutered male Chihuahua with prostatic carcinoma. Designating PAE as Day 0, we monitored the prostate, bladder, and lungs with computed tomography (CT), ultrasonography (US), and thoracic radiography for approximately 900 days. Clinical signs remained well controlled; the dog survived until Day 918. CT showed intraprostatic hypoattenuating lesions (Day 33) that enlarged with gas accumulation (Day 173); US showed hypoechoic lesions (Day 271) that became scattered hyperechoic foci (Day 678). Following PAE, the prostatic parenchymal volume decreased by approximately 33% and remained stable until Day 481. No serious PAE-related complications were observed. These findings suggest that PAE induced sustained ischemic, cavitary, and chronic remodeling changes within the prostate, which may have contributed to the prolonged local disease control observed in this dog. To our knowledge, this represents the longest time-course imaging reported after PAE in a dog with prostatic carcinoma and illustrates the potential of PAE as a feasible, minimally invasive treatment option in general veterinary practice.</p>","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":"13 ","pages":"1947473"},"PeriodicalIF":3.1,"publicationDate":"2026-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13656744/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149181411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Case Report: Computed tomographic diagnosis of asymmetric bilateral uterine horn torsion secondary to benign uterine leiomyomas in a non-gravid dog. 病例报告:未妊娠犬良性子宫平滑肌瘤继发于双侧子宫角不对称扭转的计算机断层诊断。
IF 3.1 2区 农林科学
Frontiers in Veterinary Science Pub Date : 2026-09-25 eCollection Date: 2026-01-01 DOI: 10.3389/fvets.2026.1959325
Eunseok Jeong, Jaewon Kim, Younghwan Kim, Kidong Eom, Jaehwan Kim
{"title":"Case Report: Computed tomographic diagnosis of asymmetric bilateral uterine horn torsion secondary to benign uterine leiomyomas in a non-gravid dog.","authors":"Eunseok Jeong, Jaewon Kim, Younghwan Kim, Kidong Eom, Jaehwan Kim","doi":"10.3389/fvets.2026.1959325","DOIUrl":"https://doi.org/10.3389/fvets.2026.1959325","url":null,"abstract":"<p><p>Uterine torsion is uncommon in dogs and most commonly involves a single uterine horn. An 11-year-old intact female Pomeranian was presented with a 2- to 3-day history of abdominal distension, dyspnea, anorexia, vomiting, and oliguria. Clinicopathologic findings indicated renal compromise, electrolyte abnormalities, tissue injury, and marked systemic inflammation. Radiography and ultrasonography revealed large bilateral abdominal masses with peritoneal effusion, although the organ of origin could not be determined. Contrast-enhanced computed tomography (CT) demonstrated masses arising from both uterine horns, with absent central enhancement on the right, minimal central enhancement on the left, and thin peripheral rim enhancement of both masses, suggesting vascular compromise and ischemic injury. Both ovaries showed preserved contrast enhancement, and spiral twisting of both uterine horns and bilateral whirl signs were identified, with the changes being markedly more pronounced on the right. These findings permitted a preoperative diagnosis of asymmetric bilateral uterine horn torsion. Exploratory laparotomy confirmed approximately 1,080° rotation of the right horn around the left horn and 270° rotation of the left horn around its own axis, without gross rupture of the broad ligament. En bloc ovariohysterectomy without prior detorsion and excision of the vaginal mass were performed. Histopathologic examination confirmed leiomyomas of both uterine horns and of the vaginal mass, with extensive hemorrhage and necrosis of the right horn. The dog recovered uneventfully and remained clinically well 9 months after surgery. To the authors' knowledge, this is the first report of bilateral uterine torsion associated with benign leiomyomas in a dog and the first description of bilateral whirl signs on CT in canine uterine torsion.</p>","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":"13 ","pages":"1959325"},"PeriodicalIF":3.1,"publicationDate":"2026-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13651105/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149169245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Dog-mediated active-leisure engagement and organised canine sport: a cross-sectional analysis of Hungarian dog owners. 狗介导的积极休闲参与和有组织的犬类运动:匈牙利狗主人的横断面分析。
IF 3.1 2区 农林科学
Frontiers in Veterinary Science Pub Date : 2026-09-25 eCollection Date: 2026-01-01 DOI: 10.3389/fvets.2026.1912638
Anetta Müller, Attila Lengyel, Katalin Mező, Éva Bácsné Bába, Elena Bendíková, Réka Pálinkás, Ricardo Uvinha, Ferenc Mező
{"title":"Dog-mediated active-leisure engagement and organised canine sport: a cross-sectional analysis of Hungarian dog owners.","authors":"Anetta Müller, Attila Lengyel, Katalin Mező, Éva Bácsné Bába, Elena Bendíková, Réka Pálinkás, Ricardo Uvinha, Ferenc Mező","doi":"10.3389/fvets.2026.1912638","DOIUrl":"https://doi.org/10.3389/fvets.2026.1912638","url":null,"abstract":"<p><strong>Background: </strong>Companion dogs are widely seen as a route to physical activity, yet owners differ greatly in how actively they engage their animals, and average owner-non-owner comparisons obscure this variation. We asked how strongly an owner's dog-mediated active-leisure engagement is associated with organised physical activity, and whether that engagement is patterned by place of residence and by the economic priority owners assign to their dog's health.</p><p><strong>Methods: </strong>Cross-sectional survey data from 954 Hungarian adult dog owners were analysed. Engagement was measured as the mean of ten five-point items spanning affective, dispositional, and behavioural facets (Cronbach's <i>α</i> = 0.84; McDonald's <i>ω</i> = 0.84). Lifetime participation in organised canine sport was modelled in binary logistic regression with engagement (per standard deviation), gender, capital-city residence, and age as predictors; the breadth of participation, residence, and relative health-related expenditure were examined as further outcomes. Non-linearity of the engagement effect was tested, and a latent profile analysis is reported as a supplementary comparison.</p><p><strong>Results: </strong>Engagement was approximately normally distributed (<i>M</i> = 2.99, SD = 0.83) and essentially unidimensional. Owners who had taken part in canine sport scored 0.79 standard deviations higher on engagement than those who had not. In the adjusted model, each standard deviation of engagement was associated with 2.27 times the odds of lifetime canine sport participation [95% CI (1.94, 2.64)]; the predicted probability rose from 26% one standard deviation below the mean to 65% one standard deviation above it, with a plateau above roughly 3.5 on the 1-5 scale. Capital-city residence predicted participation on its own (OR = 1.89) but not once engagement was included [OR = 1.36, 95% CI (0.87, 2.10)]. Engagement was higher in the capital than elsewhere and was positively associated with prioritising the dog's health in household spending. Among participants, the number of distinct sports undertaken was only weakly related to engagement.</p><p><strong>Discussion: </strong>Among owners, engagement rather than ownership distinguishes the physically active from the inactive, and the association is continuous across the full range of the scale rather than confined to a distinct subgroup. The potential for promoting active living through dogs therefore lies in shifting the large mid-range of the engagement distribution, where organised canine activity is still uncommon.</p>","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":"13 ","pages":"1912638"},"PeriodicalIF":3.1,"publicationDate":"2026-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13650940/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149166718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Establish a visual loop-mediated isothermal amplification (LAMP) method for rapid detection avian colibacillosis. 建立一种快速检测禽大肠杆菌病的目视环介导等温扩增(LAMP)方法。
IF 3.1 2区 农林科学
Frontiers in Veterinary Science Pub Date : 2026-09-25 eCollection Date: 2026-01-01 DOI: 10.3389/fvets.2026.1922461
Anqi Ju, Hongxiang Zhang, Guoyin Wang, Rining Zhu, Siyue Wang, Chengcheng Tan, Fangyu Zhang, Chengyu Wang, Tian Wei, Dan Xiao, Pengju Zhang
{"title":"Establish a visual loop-mediated isothermal amplification (LAMP) method for rapid detection avian colibacillosis.","authors":"Anqi Ju, Hongxiang Zhang, Guoyin Wang, Rining Zhu, Siyue Wang, Chengcheng Tan, Fangyu Zhang, Chengyu Wang, Tian Wei, Dan Xiao, Pengju Zhang","doi":"10.3389/fvets.2026.1922461","DOIUrl":"https://doi.org/10.3389/fvets.2026.1922461","url":null,"abstract":"<p><strong>Objective: </strong>To develop a rapid visual loop-mediated isothermal amplification (LAMP) assay for the detection of pathogenic <i>Escherichia coli</i> in poultry-associated samples.</p><p><strong>Methods: </strong>A total of 650 samples were collected from 11 large-scale poultry farms in Jilin Province, China, from which 632 bacterial isolates were recovered. Four sets of LAMP primers were designed based on conserved genomic regions identified by sequence alignment. Reaction temperature, incubation time, and Bst DNA polymerase dosage were optimized. The limit of detection, visual colorimetric performance, and cross-reactivity were evaluated, and the clinical diagnostic performance was compared with conventional PCR.</p><p><strong>Results: </strong>The optimized LAMP assay produced detectable amplification within 45 min at 63 °C, with a limit of detection of 3.6 CFU/mL. Positive reactions turned yellow-green, whereas negative reactions remained orange, enabling naked-eye discrimination. No cross-reactivity with non-target bacterial species was observed. The LAMP assay exhibited a 1,000-fold lower limit of detection than conventional PCR, required neither thermal cycling nor gel electrophoresis, and showed complete agreement with PCR results for clinical samples.</p><p><strong>Conclusion: </strong>This colorimetric LAMP assay is reliable and holds considerable promise as a rapid on-site screening tool for pathogenic <i>E. coli</i> in poultry-associated samples.</p>","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":"13 ","pages":"1922461"},"PeriodicalIF":3.1,"publicationDate":"2026-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13651099/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149169571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
"What if someone reads these records and judges me?": fear, legitimacy, and the hidden curriculum in veterinary clinical decision-making. “如果有人读了这些记录,对我评头论足怎么办?”恐惧,合法性,以及兽医临床决策中的隐藏课程。
IF 3.1 2区 农林科学
Frontiers in Veterinary Science Pub Date : 2026-09-25 eCollection Date: 2026-01-01 DOI: 10.3389/fvets.2026.1958601
Sara Ritzie, Jason Coe, Elizabeth C Kurucz, Lynn Henderson, Andria Jones, Lauren E Van Patter, Shane Bateman
{"title":"\"What if someone reads these records and judges me?\": fear, legitimacy, and the hidden curriculum in veterinary clinical decision-making.","authors":"Sara Ritzie, Jason Coe, Elizabeth C Kurucz, Lynn Henderson, Andria Jones, Lauren E Van Patter, Shane Bateman","doi":"10.3389/fvets.2026.1958601","DOIUrl":"https://doi.org/10.3389/fvets.2026.1958601","url":null,"abstract":"<p><strong>Introduction: </strong>Spectrum of Care (SpOC) has been promoted as a means of improving access to veterinary care while supporting ethical and sustainable clinical decision-making. Yet many veterinarians who value contextualized, client-centered care continue to experience difficulty incorporating SpOC approaches into everyday practice. While these challenges are often framed as deficits in knowledge, resources, or training, less attention has been paid to the influence of professional culture.</p><p><strong>Methods: </strong>This qualitative study drew on data collected as part of a mixed-methods evaluation of a SpOC workshop delivered to veterinarians in Ontario, Canada. Data sources included semi-structured interviews conducted 3 months following workshop participation (<i>n</i> = 17) and open-text questionnaire responses collected before, immediately after, and 3 months following the intervention. Data were analyzed using reflexive thematic analysis.</p><p><strong>Results: </strong>Participants described numerous strategies that supported contextualized care, including collaborative communication, shared decision-making, supportive team cultures, knowledge of alternative care pathways, and a growth-oriented mindset. However, fear emerged as a pervasive influence on clinical decision-making. Veterinarians described concerns about peer judgment, client complaints, regulatory scrutiny, and the possibility that context-appropriate decisions would later be interpreted as substandard care. Importantly, these concerns often reflected anticipated rather than experienced criticism. Participants linked these fears to implicit professional norms learned through veterinary training and reinforced throughout practice, describing a hidden curriculum that privileges idealized or gold-standard approaches, narrows perceptions of acceptable care, and creates pressure to conform even when alternative approaches are clinically appropriate.</p><p><strong>Discussion: </strong>These findings suggest that contextualized care may be constrained less by deficiencies in clinical knowledge than by professional and cultural forces that shape perceptions of legitimate care. Fear emerged as an immediate influence on decision-making, while the hidden curriculum functioned as a potential underlying mechanism, generating and sustaining that fear. These findings position SpOC not only as a clinical framework for improving access to care, but also as a lens through which broader questions of professional culture, legitimacy, and veterinary identity become visible. Until contextualized care is culturally legitimized alongside idealized models of care, fear and the hidden curriculum may continue to undermine efforts to improve access to care and support practitioner wellbeing.</p>","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":"13 ","pages":"1958601"},"PeriodicalIF":3.1,"publicationDate":"2026-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13651108/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149172159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Correction: A study of animal welfare in 11 species across 16 Zoos using M-AWAG and its correlations with environmental variables. 更正:使用M-AWAG对16个动物园的11个物种的动物福利及其与环境变量的相关性进行了研究。
IF 3.1 2区 农林科学
Frontiers in Veterinary Science Pub Date : 2026-09-25 eCollection Date: 2026-01-01 DOI: 10.3389/fvets.2026.1975237
Hyejin Kang
{"title":"Correction: A study of animal welfare in 11 species across 16 Zoos using M-AWAG and its correlations with environmental variables.","authors":"Hyejin Kang","doi":"10.3389/fvets.2026.1975237","DOIUrl":"https://doi.org/10.3389/fvets.2026.1975237","url":null,"abstract":"<p><p>[This corrects the article DOI: 10.3389/fvets.2026.1814857.].</p>","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":"13 ","pages":"1975237"},"PeriodicalIF":3.1,"publicationDate":"2026-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13651096/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149165723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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