Kati White, Laura Hänninen, Sanna Sainmaa, Anna Valros
{"title":"Reasons for admission and rehabilitation rates of various wildlife species in Finland.","authors":"Kati White, Laura Hänninen, Sanna Sainmaa, Anna Valros","doi":"10.3389/fvets.2024.1455632","DOIUrl":"https://doi.org/10.3389/fvets.2024.1455632","url":null,"abstract":"<p><p>Wildlife rehabilitation is a common part of animal-protection work. In Finland wildlife care is usually based on volunteer work and no licensing or training is required. Wildlife casualties are also treated professionally in some contexts such as zoos. The species of wildlife casualties may influence treatment decisions. Our anonymous online survey examined wildlife caregiving practices in Finland (<i>n</i> = 78), focusing on the care provided to various animal species and the outcomes of rehabilitation efforts. The survey was sent to both veterinarians and volunteers caring for wildlife, and it was part of a larger survey. Questions were mainly closed, and opinion-related questions were applied on a Likert scale (1-7; where 1 meant strongly disagree and 7 meant strongly agree). Most respondents primarily cared for mammals and birds. Reptiles, amphibians, and fish received less attention. Injuries and overwinter survival, especially in the case of hedgehogs, were the primary reasons for wildlife admissions. The training background of the rehabilitators varied and was related to the animal species being treated. Those caring mainly for hedgehogs (<i>Erinaceus europaeus</i>) were the least likely to have animal-related training or long-term experience in wildlife care. We show a notably high rehabilitation rate of approximately 80% of commonly treated species, significantly surpassing figures from other countries, which raises concerns that animals are admitted or released on too light grounds, leading to animal welfare problems. It is also noteworthy that only one-fifth of respondents said they kept records of animal admissions. Less than 40% of respondents emphasized the need for further education on any specific issue, which may indicate overestimation of personal skills. In conclusion, our study raises concerns regarding the ethics and potential harm associated with wildlife rehabilitation.</p>","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11479885/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142463289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ho Hyun Kwak, Sung Min Kim, Lina Yu, Jun Hyung Kim, Heung Myong Woo
{"title":"Case report: Combination technique of balloon dilation, membrane excision, and topical mitomycin C for the treatment of nasopharyngeal stenosis in a cat.","authors":"Ho Hyun Kwak, Sung Min Kim, Lina Yu, Jun Hyung Kim, Heung Myong Woo","doi":"10.3389/fvets.2024.1452002","DOIUrl":"https://doi.org/10.3389/fvets.2024.1452002","url":null,"abstract":"<p><p>A two-year-old neutered male Turkish Angora cat presented with respiratory signs, including chronic snoring sounds and dyspnea with open-mouth breathing. Nasopharyngeal stenosis (NPS) was diagnosed based on endoscopy and computed tomography (CT). An attempt was made to break down the membrane, causing stenosis in the nasopharynx through balloon dilation using a valvuloplasty balloon dilation catheter (12 mm × 3 cm) and retroflexed endoscope. The balloon size was selected according to the identified diameter of the stenotic site on nasopharyngeal CT images. The balloon was inflated with radiographic contrast medium and maintained for 2 min; the similar procedure was repeated four additional times. The stenotic membrane was excised after balloon dilation. Topical Mitomycin C (MMC) was then administered to the stenotic region. After 2 weeks, an additional MMC application was repeated to prevent recurrence. The cat remained free of clinical signs without recurrence for 12 months after the most recent procedure. In this study, effective treatment results were obtained using a combination of balloon dilation, membrane excision, and topical MMC for membranous nasopharyngeal stenosis in a cat.</p>","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11479965/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142463255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Carla Ippoliti, Pastor Alfonso, Assaf Anyamba, Beatriz Martínez-López
{"title":"Editorial: Satellite Earth Observation for animal health and vector-borne diseases.","authors":"Carla Ippoliti, Pastor Alfonso, Assaf Anyamba, Beatriz Martínez-López","doi":"10.3389/fvets.2024.1484990","DOIUrl":"https://doi.org/10.3389/fvets.2024.1484990","url":null,"abstract":"","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11479869/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142463263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Maria Alfonsa Cavalera, Oana Gusatoaia, Andrea Zatelli
{"title":"Seasonality of anti-<i>Leishmania infantum</i> titers in dogs: a crucial factor for designing effective clinical trials.","authors":"Maria Alfonsa Cavalera, Oana Gusatoaia, Andrea Zatelli","doi":"10.3389/fvets.2024.1477696","DOIUrl":"https://doi.org/10.3389/fvets.2024.1477696","url":null,"abstract":"","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11473252/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142463293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Integrated metagenomics and metabolomics analyses revealed biomarkers in β-casein A2A2-type cows.","authors":"Jinyan Zhao, Chuanchuan Wang, Jiahuan Hu, Ruoshuang Ma, Baojun Yu, Wei Zhao, Hua Wang, Yaling Gu, Juan Zhang","doi":"10.3389/fvets.2024.1438717","DOIUrl":"https://doi.org/10.3389/fvets.2024.1438717","url":null,"abstract":"<p><p>In Holstein cows, β-casein, one of the most critical proteins in milk, exists in two main genotypes, A1 and A2. Herein, 45 Holstein cows [categorized into three groups based on β-casein A1A1, A1A2, and A2A2 genotypes (<i>N</i> = 15)] with the same feeding management and litter size were enrolled to explore differences in rumen microflora and metabolites across various β-casein genotypes. Rumen fluids were collected for metagenomics and metabolomics analyses. Metabolomics and weighted gene co-expression network analysis (WGCNA) revealed that arachidonic acid (AA), adrenic acid (AdA), glycocholic acid (GCA), and taurocholic acid (TCA) were significantly and positively correlated with milk fat % in dairy cows (<i>p</i> < 0.05). Furthermore, macro-genomics and Spearman's correlation analysis revealed significant positive correlations (<i>p</i> < 0.05) between the characteristic flora (<i>g_Acetobacter</i>, <i>g_Pseudoxanthomonas</i>, <i>g_Streptococcus</i>, and <i>g_Pediococcus</i>) and the five characteristic metabolites in the rumen of A2A2 dairy cows. Moreover, functional enrichment analysis revealed more genes enriched to the TRP channel's inflammatory mediator-regulated pathway and the mTOR signaling pathway in A2A2 genotyped cows. Additionally, the regulatory effects of AA on bovine mammary epithelial cells (BMECs) were examined using CCK-8, EdU, and qRT-PCR assays, revealing that AA promoted triglyceride (TG) synthesis and upregulated the milk fat marker genes including <i>SREBF1</i>, <i>ACSS2</i>, <i>AGPAT6</i>, and <i>FASN</i>. Overall, we identified characteristic microorganisms and metabolites in A2A2 Holstein cows and established that AA could be a biomarker for higher milk fat %.</p>","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11475472/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142463268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Meaghan E Bride, Francesca Samarani, Lauren E Grant, Fiona M K James
{"title":"Canine epilepsy/seizure occurrence in primary care and referral populations: a look into the epidemiology across countries.","authors":"Meaghan E Bride, Francesca Samarani, Lauren E Grant, Fiona M K James","doi":"10.3389/fvets.2024.1455468","DOIUrl":"https://doi.org/10.3389/fvets.2024.1455468","url":null,"abstract":"<p><p>Epilepsy is a common neurological condition in dogs. Analysis of primary care populations across countries can provide a more complete understanding of the epidemiology of this condition and provide context for spectrum of care discussions. This narrative literature review was aimed at understanding canine epilepsy/seizure prevalence in primary care populations, and changes in occurrence across geography, culture, and socioeconomic status. There are few studies to give insight into the true general population of epileptic canines and there is inconsistency in the literature regarding the standards applied for epilepsy diagnosis across primary care and referral practices. Therefore, the future focus should be on more epidemiological research in primary care and mixed populations, more veterinary education to standardize use of medical guidelines in primary care settings, and increased awareness of the benefits of having pet insurance to mitigate the potentially substantial cost of care for dogs with epilepsy.</p>","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11473256/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142486160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ulrike Lessiak, Maria Melchert, Ingrid Walter, Stefan Kummer, Barbara Nell, Waltraud Tschulenk, Barbara Pratscher
{"title":"Isolation-protocol, characterization, and <i>in-vitro</i> performance of equine umbilical vein endothelial cells.","authors":"Ulrike Lessiak, Maria Melchert, Ingrid Walter, Stefan Kummer, Barbara Nell, Waltraud Tschulenk, Barbara Pratscher","doi":"10.3389/fvets.2024.1421946","DOIUrl":"https://doi.org/10.3389/fvets.2024.1421946","url":null,"abstract":"<p><p>Angiogenesis plays a crucial role in various physiological and pathological conditions. However, research in equine angiogenesis is relative limited, necessitating the development of suitable <i>in-vitro</i> models. To effectively analyze angiogenesis in-vitro, it is essential to target the specific cells responsible for this process, namely endothelial cells. Human umbilical vein endothelial cells (HUVECs) are one of the most used <i>in vitro</i> models for studying angiogenesis in humans. Serving as an equivalent to HUVECs, we present a comprehensive isolation protocol for equine umbilical vein endothelial cells (EqUVECs) with relatively minimal requirements, thereby enhancing accessibility for researchers. Umbilical cords obtained from five foals were used to isolate endothelial cells, followed by morphological and immunohistochemical identification. Performance of the cells in various assays commonly used in angiogenesis research was studied. Additionally, EqUVEC expression of vascular endothelial growth factor (VEGF) was assessed using ELISA. EqUVECs exhibited endothelial characteristics, forming a homogeneous monolayer with distinctive morphology. Immunohistochemical staining confirmed positive expression of key endothelial markers including von Willebrand factor (vWF), CD31, and vascular endothelial growth factor receptor-2 (VEGFR-2). Furthermore, performance assessments in <i>in-vitro</i> assays demonstrated the viability, proliferation, migration, tube formation and VEGF-expression capabilities of EqUVECs. The findings suggest that EqUVECs are a promising <i>in-vitro</i> model for studying equine angiogenesis, offering a foundation for further investigations into equine-specific vascular processes and therapeutic interventions.</p>","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11473255/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142463269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yang Gao, Xuexi Yang, Bo Chen, Huan Leng, Jize Zhang
{"title":"The biological function of <i>Urtica</i> spp. and its application in poultry, fish and livestock.","authors":"Yang Gao, Xuexi Yang, Bo Chen, Huan Leng, Jize Zhang","doi":"10.3389/fvets.2024.1430362","DOIUrl":"https://doi.org/10.3389/fvets.2024.1430362","url":null,"abstract":"<p><p><i>Urtica</i> species is an angiosperm plant in the Urticaceae family. It serves as a traditional food and medicinal herb, possessing high nutritional value and various bioactive compounds, including polysaccharides, flavonoids, and polyphenolic compounds. In the realm of animal feeds, <i>Urtica</i> spp. can replace traditional protein feed sources and high-quality forage, thereby reducing feed costs. Moreover, <i>Urtica</i> spp. extract exhibits antioxidant and anti-inflammatory properties and boosts immune regulation. Hence, <i>Urtica</i> spp. plays a beneficial role in enhancing animal performance and improving their immune function. Recently, with the development of sustainable farming techniques, the demand for feed additives that prioritize safety, the absence of drug residues, and environmental friendliness have grown. Consequently, <i>Urtica</i> spp. and its extracts have received widespread attention in animal production. This article summarizes the biological functions of <i>Urtica</i> spp. and its application in animal husbandry while also outlining future prospects for its application. It will provide a scientific basis and reference point for the application of <i>Urtica</i> spp. in animal health and breeding.</p>","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11473253/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142463294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Young Beom Kwak, Ilia Stambler, Hye Hyun Yoo, Jungho Yoon
{"title":"Metabolomic analysis of the impact of red ginseng on equine physiology.","authors":"Young Beom Kwak, Ilia Stambler, Hye Hyun Yoo, Jungho Yoon","doi":"10.3389/fvets.2024.1425089","DOIUrl":"https://doi.org/10.3389/fvets.2024.1425089","url":null,"abstract":"<p><strong>Introduction: </strong>Red ginseng (RG), a traditional herbal remedy, has garnered attention owing to its diverse health benefits resulting from its complex composition. However, extensive research is needed to substantiate the efficacy of RG and understand the underlying mechanisms supporting these benefits. This study aimed to identify potential biomarkers and investigate the impact of RG on related metabolic pathways in horse plasma using liquid chromatography-mass spectrometry (LC-MS)-based metabolomics.</p><p><strong>Methods: </strong>Ten horses were divided into control and RG groups, with the latter administered RG at a dose of 600 mg⋅kg<sup>-1</sup>⋅day<sup>-1</sup> for 3 weeks. Subsequently, the plasma samples were collected and analyzed using LC-MS. Multivariate statistical analysis, volcano plots, and feature-based molecular networking were employed.</p><p><strong>Results: </strong>The analysis identified 16 metabolites that substantially decreased and 21 metabolites that substantially increased following RG consumption. Among the identified metabolites were oleanolic acid, ursolic acid, and ginsenoside Rb1, which are known for their antioxidant and anti-inflammatory properties, as well as lipid species that influence sphingolipid and glycerophospholipid metabolism. Additionally, potential biomarkers, including major RG components, demonstrated distinct group clustering in principal component analysis and partial least squares-discriminant analysis, indicating their utility in assessing the physiological effects of RG consumption.</p><p><strong>Discussion: </strong>This study contributes to a comprehensive understanding of the effects of RG on health.</p>","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11471734/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142463273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Antoni Dalmau, Lorena Padilla, Aranzazu Varvaró-Porter, Aida Xercavins, Antonio Velarde, Alexandra Contreras-Jodar
{"title":"Animal welfare assessment protocol for quails reared for meat production.","authors":"Antoni Dalmau, Lorena Padilla, Aranzazu Varvaró-Porter, Aida Xercavins, Antonio Velarde, Alexandra Contreras-Jodar","doi":"10.3389/fvets.2024.1452109","DOIUrl":"https://doi.org/10.3389/fvets.2024.1452109","url":null,"abstract":"<p><strong>Introduction: </strong>It is estimated that 1.4 billion quails are reared each year for their eggs and meat, but animal welfare assessment protocols for this species have yet to be established. The objective of this study was to devise an animal welfare assessment protocol developed through a multidimensional approach that contained a number of animal-based indicators (ABIs) for quails (<i>Coturnix japonica</i>) reared for meat production.</p><p><strong>Methods: </strong>During 2021 and 2022, the identical auditor visited and audited 14 Spanish farms in their initial year of integration into an animal welfare certification scheme. The protocol is categorised into 4 principles and 12 criteria. The \"good feeding\" principle includes 6 indicators (1 ABI), \"good housing\" includes 10 indicators (5 ABIs), \"good health\" includes 12 indicators (9 ABIs), and \"appropriate behaviour\" contains 8 indicators (5 ABIs). The final welfare assessment is calculated at the farm level using scores from the on-farm recordings. The assessment is a step-by-step weighted sum of the scores from the various indicators, with the final score ranging between 0 and 100.</p><p><strong>Results and discussion: </strong>The main welfare issues found on all farms were a lack of temperature and humidity records, a poor lighting pattern, and the absence of an outdoor range or access to one. To a lesser degree, it was also found that there were excessive numbers of birds per feeder, the presence of improperly functioning drinkers (i.e., not working, inadequate water flow, or dripping water), poor litter quality, and a high prevalence of birds with dirty plumage and lameness. Despite this, the farms achieved a good overall score, being classified as \"enhanced\" (<i>n</i> = 11) and \"acceptable\" (<i>n</i> = 3). The tool proved helpful in identifying specific welfare issues at the farm level and conducting benchmarking.</p>","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11471728/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142463253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}