Frontiers in Veterinary Science最新文献

{"title":"First opinion practice electronic health records are a useful source of descriptions of medication errors.","authors":"Eirini Petrou, Heather Davies, Maria Aoun, Alan D Radford, David Singleton, Peter-John M Noble, David R Killick","doi":"10.3389/fvets.2025.1560652","DOIUrl":"10.3389/fvets.2025.1560652","url":null,"abstract":"<p><strong>Background: </strong>Medication error (MedE) is a leading global cause of harm in human healthcare with significance both in patient morbidity and mortality, and consequent legal and financial issues. Despite this, MedEs are a poorly explored area in veterinary medicine. Research has so far focussed on survey work and errors spontaneously reported to third parties, such as professional indemnity providers.</p><p><strong>Aim: </strong>Determine if MedEs can be successfully identified in first opinion electronic health records (EHRs).</p><p><strong>Animals: </strong>EHRs pertaining to animals treated in UK first opinion practice.</p><p><strong>Materials and methods: </strong>Regular expressions (REGEX) were designed (with assistance from a domain expert) to identify explicit reference to MedEs in the SAVSNET EHR dataset. Identified MedEs were then classified by the linear sequence of medication therapy, the degree of harm caused, the role of the person who made the error, and the medication type involved.</p><p><strong>Results: </strong>In total, 6,665 EHRs were identified by the REGEX, of which a random 2,847 were manually reviewed, with 1,023 (35.9%) matching the MedEs case definition. Of these MedEs, 29.5% (<i>n</i> = 302) caused mild harm to the patient, 2.8% (<i>n</i> = 27) moderate harm and 0.2% (<i>n</i> = 2) severe harm. MedEs were most frequent during the \"drug administered\" phase (51.4%) and within this phase, \"dosing errors\" were most common (68.1%). The most common medication types, associated with \"drug administered\" phase MedEs were vaccinations (27.1%) and non-steroidal anti-inflammatory drugs (19.0%).</p><p><strong>Conclusion: </strong>EHRs are a useful source of data on MedEs. MedEs are a common cause of patient harm in veterinary practice. The data provided here highlights drug classes at higher risk of problems for which mitigating action and/or education interventions are indicated.</p>","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":"12 ","pages":"1560652"},"PeriodicalIF":2.6,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12130827/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144215658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"QRS complex configurations in 12-lead electrocardiograms of dogs with monomorphic ventricular tachycardia or complete bundle branch block.","authors":"Manuela Perego, Alessandra Maffei, Damiano Cavallini, Roberto Santilli","doi":"10.3389/fvets.2025.1579951","DOIUrl":"10.3389/fvets.2025.1579951","url":null,"abstract":"<p><strong>Introduction: </strong>The differentiation between ventricular tachycardias (VT) and supraventricular tachycardias (SVT) with bundle branch block (BBB) is clinically challenging. The aim of the study was to define by the 12-lead-electrocardiogram the QRS complex morphology in monomorphic VT (MVT) and in BBB.</p><p><strong>Methods: </strong>Twelve-lead-electrocardiograms were blindly retrospectively analyzed and categorized in four groups: sinus rhythm with left bundle branch block (SR-LBBB), sinus rhythm with right bundle branch block (SR-RBBB), MVT with RBBB configuration (MVT-RBBB), MVT with LBBB configuration (MVT-LBBB). Measurements were not normally distributed, and they were normalized by Box-Cox transformation. Repeated-measures linear mixed-effects models were constructed according to the 3 measurements performed.</p><p><strong>Results: </strong>A total of 103 12-lead-electrocardiograms were retrospectively analyzed: 18 SR-RBBB, 18 SR-LBBB, 33 MVT-RBBB, 34 MVT-LBBB. Limb leads concordance was found in 100% of SR-RBBB, 100% of SR-LBBB, 54.5% of MVT-RBBB, 70.6% of MVT-LBBB. Precordial leads discordance was present in 100% of SR-RBBB, 100% of SR-LBBB, 78.8% of MVT-RBBB, 88.2% of MVT-LBBB. The transition point was located at V1-V2 in 100% of SR-RBBB, 100% of SR-LBBB, 50.5% of MVT-RBBB and 71.5% of MVT-LBBB. Positive V1 with M shape morphology was detected in 100% of SR-RBBB and 12% of MVT-RBBB. The mean electrical axis on the frontal plane was -108.15° (-118.29 / -101.52) in RSSR-RBBB, 75.42° (71.78-80.46) in RSSR -LBBB, -93.46° (-102.75/-78.49) in MVT-RBBB and 82.27° (76.85-88.95) in MVT-LBBB.</p><p><strong>Discussion: </strong>In case of inability to identify signs of atrioventricular dissociation, the presence of standard limb leads discordance, precordial leads concordance or discordance with transition point other than V1-V2, left limb leads and left precordial leads discordance, aVR and V1 discordance and absence of M shape configuration of the QRS complex in lead V1 is likely to be MVT.</p>","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":"12 ","pages":"1579951"},"PeriodicalIF":2.6,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12129992/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144215674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Case Report: Idiopathic intramural hematoma of the jejunum in a French Bulldog.","authors":"Yeon-Jin Kim, Dong-Min Choi, Jong-Il Kang, Dong-Yeong Kim, Chan-Sik Nam, Kwang-Sup Lee, Hee-Myung Park","doi":"10.3389/fvets.2025.1577989","DOIUrl":"10.3389/fvets.2025.1577989","url":null,"abstract":"<p><p>This case report describes clinical and diagnostic features of an idiopathic jejunal intramural hematoma in a dog presenting with non-specific gastrointestinal symptoms. A 2-year-old neutered male French Bulldog was presented with vomiting, abdominal pain, and chronic soft stools persisting for 2 weeks, with no history of trauma or dietary indiscretion. Physical examination revealed abdominal distension and palpable discomfort. The complete blood count (CBC) showed leukocytosis with neutrophilia, accompanied by signs of dehydration. Serum chemistry revealed no significant abnormalities, although mild electrolyte imbalances were observed. The canine pancreatic lipase immunoreactivity (cPLI) test was within the reference range and the C-reactive protein (CRP) level was slightly elevated. Ultrasonography revealed a heterogeneous mass in the jejunal region, which was suspected to be a hematoma. An exploratory laparotomy was performed, and the affected jejunal segment was surgically resected. Histopathological examination confirmed the mass as an intramural hematoma. Additionally, coagulation profiles revealed no remarkable findings. The cause of the hematoma was determined to be idiopathic after excluding other possible causes. This case highlights the importance of considering intramural hematomas in the differential diagnosis for dogs presenting with non-specific gastrointestinal symptoms, even in the absence of trauma and coagulation abnormalities.</p>","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":"12 ","pages":"1577989"},"PeriodicalIF":2.6,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12129902/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144215654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary supplementation with soluble corn fiber improved fecal score, microbiota, and SCFAs in dogs.","authors":"Donghui Liang, Shuai Zhao, Guoan Yin","doi":"10.3389/fvets.2025.1599213","DOIUrl":"10.3389/fvets.2025.1599213","url":null,"abstract":"<p><strong>Introduction: </strong>Soluble corn fiber, a safe dietary fiber with prebiotic properties, has been put to several applications in human daily life. However, studies on its use in pet food are scarce. This study was conducted to investigate the effects of SCF on microbial diversity, SCFAs and fecal quality in canines.</p><p><strong>Methods: </strong>Twenty adult dogs were divided into four groups, including the control group (CON) and three groups fed diet supplemented with 0.1% (SCF1), 0.5% (SCF2), or 1% (SCF3) SCF for 21 days.</p><p><strong>Results: </strong>Fecal scores of the group fed 1% SCF were the closest to the ideal state. <i>α</i>-diversity analysis showed the Chao1 index in the SCF2 and SCF3 groups was significantly higher (<i>p</i> < 0.05) than in the CON group, indicating an increase in colony abundance. <i>β</i>-diversity analysis showed no significant structural difference among groups (<i>p</i> > 0.05). Microbial diversity analysis showed the addition of SCF to the diets increased the relative abundance of <i>Bacteroidota</i> and <i>Blautia</i> and decreased the relative abundance of <i>Firmicutes</i>, <i>[Ruminococcus]_gnavus_group</i>, and <i>Prevotellaceae_Ga6A1_group</i>; 1% SCF the relative abundance of <i>Prevotella</i> and <i>Blautia</i> (<i>p</i> < 0.05), and the content of acetic acid, valeric acid, and isobutyric acid (<i>p</i> < 0.05) and significantly decreased the content of butyric acid (<i>p</i> < 0.05).</p><p><strong>Conclusion: </strong>Dietary supplementation with SCF improves the fecal condition, modulates microbiota composition, enhances the levels of acetic acid, valeric acid, and isobutyric acid, and decreases the level of butyric acid in dogs, with optimal effects observed for 1% supplementation.</p>","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":"12 ","pages":"1599213"},"PeriodicalIF":2.6,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12131326/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144215655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation and identification of an AKAV strain in dairy cattle in China.","authors":"Miaomiao Zhang, Menghua Deng, Sisi Zhao, Dengshuai Zhao, Yajie Zheng, Limei Qin, Han Gao, Mengmeng Zhao, Keshan Zhang","doi":"10.3389/fvets.2025.1574667","DOIUrl":"10.3389/fvets.2025.1574667","url":null,"abstract":"<p><p>Akabane disease is an arthropod-borne disease caused by Akabane virus (AKAV), which is characterized by abortion, premature birth, stillbirth, congenital arthrosis, and hydrocephalic anencephalic syndrome in pregnant cattle and sheep. The occurrence of AKAV was proved by RT-PCR amplification based on AKAV S fragment, virus isolation, cells inoculation, cytopathy, transmission electron microscopy, and gene sequencing. The PCR amplicon was approximately 850 bp and was sequenced, and molecular identification of AKAV was conducted through phylogenetic analysis of S gene sequence. The results indicated that AKAV isolated from cattle in this study was genetically close to the strain isolated from <i>Rhizomys pruinosus</i> in China in 2016. However, the outbreak in bamboo rats may have been a sporadic event. The probability that Akabane virus (AKAV) can spread in rodents and mammals is still uncertain and requires further investigation. Using transmission electron microscopy (TEM), AKAV particles displayed the typical morphology associated with bunyaviruses reported previously. In brief, the AKAV infection in cattle has been confirmed. This case report highlights the necessity for enhanced surveillance and preventive measures to mitigate the potential impact on livestock health and productivity.</p>","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":"12 ","pages":"1574667"},"PeriodicalIF":2.6,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12131916/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144215659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Case Report: Clinical and histopathological findings of porcelain gallbladder in a dog.","authors":"Tae-Young Kim, Ye-In Oh","doi":"10.3389/fvets.2025.1570221","DOIUrl":"10.3389/fvets.2025.1570221","url":null,"abstract":"<p><p>Porcelain Gallbladder (PGB) is a condition marked by extensive calcification and thickening of the gallbladder wall. PGB is extremely rare in dogs. The exact mechanism of PGB remains unclear. However, chronic cholecystitis and factors such as biliary hyperplasia, mucin hypersecretion, and cystic duct obstruction can lead to fibrosis and calcification of the gallbladder wall, potentially resulting in PGB. A 13-year-old spayed female Shih Tzu presented with anorexia, lethargy, vomiting, and weight loss. Physical exams showed mild epigastric pain. Blood tests indicated mild alanine aminotransferase (ALT) elevation, significant alkaline phosphatase, and gamma-glutamyl transferase (GGT) increases. Hyperlipidemia, hypercholesterolemia, and elevated canine pancreatic lipase (cPL) were also noted. Diagnostic imaging revealed extensive gallbladder wall calcification and thickening, choleliths, mild bile duct dilation, pancreatitis, and duodenitis. Cholecystectomy was performed, and symptomatic treatment for pancreatitis and duodenitis was administered. Histopathological examination is essential for confirming PGB by identifying calcification of the gallbladder wall. Histopathological examination of the gallbladder revealed severe papillary hyperplasia, mucin hypersecretion, multiple ulcers, diffuse calcification, and fibrotic changes. These findings confirmed the diagnoses of gallbladder mucocele and PGB. Postoperatively, biochemical markers normalized or significantly decreased, with clinical improvement observed. This study presents the rare occurrence of PGB in dogs and emphasizes the importance of appropriate veterinary intervention for improving clinical outcomes.</p>","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":"12 ","pages":"1570221"},"PeriodicalIF":2.6,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12130736/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144215653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"On the potential of agentic workflows for animal training plan generation.","authors":"Jörg Schultz","doi":"10.3389/fvets.2025.1563233","DOIUrl":"https://doi.org/10.3389/fvets.2025.1563233","url":null,"abstract":"<p><p>Effective animal training depends on well-structured training plans that ensure consistent progress and measurable outcomes. However, the creation of such plans is often time-intensive, repetitive, and detracts from hands-on training. Recent advancements in generative AI powered by large language models (LLMs) provide potential solutions but frequently fail to produce actionable, individualized plans tailored to specific contexts. This limitation is particularly significant given the diverse tasks performed by dogs-ranging from working roles in military and police operations to competitive sports-and the varying training philosophies among practitioners. To address these challenges, a modular agentic workflow framework is proposed, leveraging LLMs while mitigating their shortcomings. By decomposing the training plan generation process into specialized building blocks-autonomous agents that handle subtasks such as structuring progressions, ensuring welfare compliance, and adhering to team-specific standard operating procedures (SOPs)-this approach facilitates the creation of specific, actionable plans. The modular design further allows workflows to be tailored to the unique requirements of individual tasks and philosophies. As a proof of concept, a complete training plan generation workflow is presented, integrating these agents into a cohesive system. This framework prioritizes flexibility and adaptability, empowering trainers to create customized solutions while leveraging generative AI's capabilities. In summary, agentic workflows bridge the gap between cutting-edge technology and the practical, diverse needs of the animal training community. As such, they could form a crucial foundation for advancing computer-assisted animal training methodologies.</p>","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":"12 ","pages":"1563233"},"PeriodicalIF":2.6,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12130733/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144215660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isoenergetic reduction of dietary macronutrients affects body composition, physical activity, and post-prandial hormone responses in lean and obese cats fed to maintain body weight.","authors":"Hannah Godfrey, Érico de Mello Ribeiro, Shoshana Verton-Shaw, Anna Kate Shoveller, Darcia Kostiuk, Janelle Kelly, Jennifer Saunders Blades, Adronie Verbrugghe","doi":"10.3389/fvets.2025.1588330","DOIUrl":"10.3389/fvets.2025.1588330","url":null,"abstract":"<p><strong>Introduction: </strong>High consumption of dietary carbohydrates (nitrogen-free extract, NFE) in extruded dry foods is postulated as a risk factor for feline obesity, though evidence is limited. This study utilized a three-test diet approach to isolate the effect of each macronutrient on body composition, voluntary physical activity, and serum satiety hormone response in lean and obese cats.</p><p><strong>Materials and methods: </strong>A pairwise isoenergetic reduction as % metabolizable energy (ME, using modified Atwater) of dietary macronutrients created a low-protein (LP; protein = 28%, fat = 40%, NFE = 32%ME), low-fat (LF; protein = 40%, fat = 30%, NFE = 30%ME), and low-carbohydrate (LC; protein = 36%, fat = 41%, NFE = 23%ME) diet. Cats (lean <i>n</i> = 9; obese <i>n</i> = 9) were fed the LP, LF, or LC diet to maintain body weight for 4-weeks in a 3 × 3 Latin square design. Voluntary physical activity was measured from day 15-21. On day 23, body composition was assessed by dual energy x-ray absorptiometry. On day 24, blood was collected for fasted and 6-h post-prandial serum satiety hormones. Data were analysed using SAS Studio via proc. GLIMMIX with significance set at <i>p</i> < 0.05.</p><p><strong>Results: </strong>Greater lean soft tissue mass was observed for LF compared to LP and LC (<i>p</i> = 0.0101) though no other changes in body composition were observed. Daily food intake (g/d) differed among diets (LP: 56.14 ± 0.56; LF: 59.52 ± 0.59; LC: 56.50 ± 0.56; <i>p</i> = 0.0003) though energy intake (kcal/d) was similar (LP: 218.47 ± 2.32; LF: 220.42 ± 2.34; LC: 225.37 ± 2.39; <i>p</i> = 0.1076). Voluntary physical activity was similar for lean and obese cats (<i>p</i> = 0.4025). LP cats exhibited less physical activity during dark hours compared to LF and LC (<i>p</i> = 0.0155). No body condition effect was observed for serum leptin or ghrelin (<i>p</i> = 0.6243 and <i>p</i> = 0.6747). Obese cats on LP exhibited a lower serum leptin iAUC compared to obese cats on LC (P_BC*Diet = 0.0093). Post-prandial serum ghrelin decreased in all cats whereas serum peptide YY (PYY) increased at 1-, 2-, and 3-h post-prandial (P_Time = 0.0094 and P_Time = 0.0050). Serum ghrelin was lower at 6-h post-prandial (P_Diet*Time = 0.0030) and serum PYY was greater at 1-h post-prandial (P_Diet*Time = 0.0086) for LC.</p><p><strong>Discussion: </strong>Overall, the LP, LF, and LC diets were not associated with differences in most parameters, supporting that cats are metabolically flexible if nutrient requirements are met. There may be benefits regarding satiety hormone responses to a LC diet in cats though more research is required.</p>","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":"12 ","pages":"1588330"},"PeriodicalIF":2.6,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12128646/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144208357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular identification of zoonotic taeniids metacestodes in several rodent species trapped in Central Europe.","authors":"Tomáš Husák, Zuzana Čadková, Ondřej Máca, Marek Kouba, Zdeňka Klimková, Richard Sehnal, Jana Nápravníková, Václava Hrabětová, Ivana Jankovská, Jaroslav Vadlejch, Iva Langrová","doi":"10.3389/fvets.2025.1571082","DOIUrl":"10.3389/fvets.2025.1571082","url":null,"abstract":"<p><strong>Background: </strong>Larval stages of carnivore cestodes in rodents have been extensively studied for decades, primarily based on morphological indicators. Numerous datasets, particularly from Central Europe, exist on this topic. Traditionally, the shape, number, and size of hooks have been key distinguishing features. However, recent findings suggest that morphology alone may not provide accurate identification. In this study, rodent livers from various habitats across five regions of the Czech Republic were examined for the presence of taeniid larval cestodes.</p><p><strong>Methods: </strong>A total of 2,934 rodent specimens were collected using snap traps between 2014 and 2022. Taeniid metacestodes detected in these intermediate hosts were subsequently subjected to species determination through molecular (targeting the cytochrome oxidase subunit I gene) and morphological examinations.</p><p><strong>Results: </strong>The following cestodes were identified using molecular methods: <i>Hydatigera kamiyai</i> (found in <i>Apodemus flavicollis</i>, <i>Apodemus sylvaticus</i>, <i>Microtus arvalis</i>, and <i>Microtus agrestis</i>), <i>Taenia martis</i> (in <i>A. flavicollis</i>, <i>M. arvalis</i>, and <i>Myodes glareolus</i>), and <i>Versteria mustelae</i> (in <i>M. arvalis</i> and <i>My</i>. <i>glareolus</i>). Sequencing of 55 PCR-positive isolates revealed 13 haplotypes of <i>H</i>. <i>kamiyai</i>, one of <i>T</i>. <i>martis</i> and four of <i>V</i>. <i>mustelae</i>. Further, we also identified co-infection by more metacestode species or haplotypes. No rodent liver samples tested were positive for <i>Echinococcus multilocularis</i> or <i>Hydatigera taeniaeformis</i> sensu stricto, either through morphological or molecular identification methods. Furthermore, no other cryptic species were detected during this study.</p><p><strong>Conclusion: </strong>This study presents the first comprehensive molecular data on the aforementioned zoonotic cestode species in the Czech Republic. The findings demonstrate the importance of using molecular techniques for Taeniidae species determination, as morphological methods may lead to erroneous classifications. Furthermore, this research highlights the importance of accurate diagnostic techniques, enabling the development of effective prevention and control strategies by verifying host-parasite relationships.</p>","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":"12 ","pages":"1571082"},"PeriodicalIF":2.6,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12127760/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144208358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Screening of functional genes affecting the quality of translucent eggshell membranes based on RNA-seq analysis and DIA proteomics.","authors":"Chun-Hao Han, Xiao-Yu Zhao, Chuan-Wen Wang, Li-Jun Xu, Hua-Ge Liu, Hui Chen, Rong-Yan Zhou, De-He Wang","doi":"10.3389/fvets.2025.1583291","DOIUrl":"10.3389/fvets.2025.1583291","url":null,"abstract":"<p><p>The quality of eggshells holds substantial economic significance and serves as a critical selection criterion in poultry breeding. Eggshell translucency significantly impairs their aesthetic quality, which is structurally attributed to the thinning of the eggshell membrane or reduced tensile strength. In this study, 836 dwarf white hens were selected, with 45 hens each assigned to the opaque group and the translucent group. Grading for eggshell translucency was conducted at 75, 80, and 85 weeks of age. Based on the results from these three gradings, 35 hens that consistently produced translucent eggs and 35 hens that consistently produced opaque eggs were reclassified into the translucent group and the opaque group, respectively. The thickness of the eggshell membrane, latitudinal and longitudinal tensile force and length, and other indicators related to eggshell membrane quality were measured. Correlation analysis was performed using RNA-seq genomics and DIA proteomics based on the relationships among these indicators. Transcriptome analysis revealed 179 significantly differentially expressed genes, indicating that the causes of translucent eggshells are associated with metabolism, signal transduction, the immune system, molecular binding, transport, and catabolism. Seven potential candidate genes, including <i>CACNA2D2</i> and <i>KIF26A</i>, were identified. Proteomics analysis identified 565 differentially expressed proteins, suggesting that the expression of eggshell membrane proteins in translucent eggs is linked to protein metabolism, amino acid synthesis, immune regulation, metabolism and oxidative stress, catalytic activity, and molecular transport. Candidate proteins such as <i>SLC4A1</i>, <i>APOA1</i>, and <i>APOA4</i> were also identified. Combined proteomics and transcriptomics analysis identified 42 differentially expressed proteins and genes, including candidate genes such as <i>CA2</i>, <i>LUZP2</i>, <i>OVA</i>, <i>OVALY</i>, <i>OVST</i>, and <i>AVBD11</i>. This study provides a foundation for elucidating the genetic regulatory mechanisms underlying eggshell membrane quality and offers valuable references and directions for further enhancing eggshell quality.</p>","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":"12 ","pages":"1583291"},"PeriodicalIF":2.6,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12128419/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144208359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}