Frontiers in Veterinary Science最新文献

{"title":"Identification of metastasis-associated protein 1 (MTA1) as a new molecular marker for canine urothelial carcinoma.","authors":"Gisella Campanelli, Noah Waxner, Nema Parkhomovsky, Chun Kuen Mak, Ji-Hang Yin, Susanne Je-Han Lin, Raphael Vanderstichel, Ching Yang, Anait S Levenson","doi":"10.3389/fvets.2025.1527167","DOIUrl":"https://doi.org/10.3389/fvets.2025.1527167","url":null,"abstract":"<p><strong>Background: </strong>Although metastasis-associated protein 1 (MTA1) is known to play a role in cancer invasion and metastasis of various cancers, the clinical significance of its expression in canine urothelial carcinoma (UC) has not been explored. We sought to evaluate the expression of MTA1, cyclooxygenase 2 (COX2) and E-cadherin (E-cad) in association with clinicopathological parameters in clinical samples of canine UC.</p><p><strong>Methods: </strong>We retrospectively analyzed UC tissues from 28 canine patients using immunohistochemistry for Ki67, CD31, MTA1, COX2, and E-cad staining. Statistical significance for marker staining intensities was evaluated by ANOVA or Student's <i>t</i>-test. The correlation between molecular markers in canine UC samples detected by IHC and clinicopathological features was calculated by the Wilcoxon (Mann-Whitney) and Kruskal-Wallis tests. Western blot analysis was performed for detection of EMT markers in canine cell lines.</p><p><strong>Results: </strong>We show that MTA1 and COX2 are overexpressed in canine UC samples compared to normal canine bladder samples, whereas E-cad levels are higher in normal bladder. The results demonstrated that MTA1 expression correlated with aggressive clinicopathological features such as high tumor-grade, muscular/vascular invasion, and metastasis. The expression of MTA1 differed in tumors depending on their localization, with the highest being in the urethra adjoining the prostate. Unexpectedly, higher E-cad levels were detected in metastatic tumor cells compared to primary tumor cells.</p><p><strong>Conclusion: </strong>These findings suggest that MTA1 may represent a key upstream effector tightly associated with COX2 and E-cad-mediated events in canine UC. Accordingly, MTA1 may be considered a feasible interceptive and therapeutic target for canine UC treatment.</p>","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":"12 ","pages":"1527167"},"PeriodicalIF":2.6,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12062746/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143985109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Histopathological and radiographic characterization of the lesions of pododermatitis in sheep: support for the establishment of the foot injuries degree and its prognosis.","authors":"Caroline da Silva Silveira, Raissa Moreira de Morais, Pedro Araújo Damboriarena, Ricardo Pozzobon, Martín Fraga, Bruno Leite Dos Anjos","doi":"10.3389/fvets.2025.1567665","DOIUrl":"https://doi.org/10.3389/fvets.2025.1567665","url":null,"abstract":"<p><p>Foot diseases in small ruminants cause locomotor disorders, leading to significant economic, productive, and health concerns in sheep and goat farming worldwide. The diagnosis and classification of lesions caused by footpad dermatitis are complex and based only on clinical observations in the field. In this context, this study assessed the histopathological and radiographic characteristics of lesions caused by pododermatitis in sheep to improve and deepen the classification of lesions and optimize diagnosis and prognosis. In this study 1.701 lame sheep were included and were distributed across 21 farms in southern Brazil. Lesions were categorized into three severity grades based on clinical, histological and radiographic observations. As a result of these findings, the lesions were categorized into 3 grades described as: mild interdigital dermatitis (grade 1), necrosis with bone involvement (grade 2) and severe tissue loss with osteolysis (grade 3). Radiographic evaluation revealed bone changes ranging from mild inflammation to osteomyelitis and pathological fractures in advanced grades. Histologically, in most severe cases, progressive inflammation, thrombosis and necrosis were observed. The results suggest that regardless of the origin of pododermatitis, whether related to environmental factors and/or agents such as <i>Dichelobacter nodosus</i> and <i>Fusobacterium necrophorum</i>, the lesions are progressive and severe. This adapted classification system can help field technicians and producers to effectively diagnose and treat these lesions depending on the grade, limiting their progression and consequently reducing economic losses. This integrated approach can improve animal welfare and productivity in South American herds, where these diseases are a significant concern.</p>","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":"12 ","pages":"1567665"},"PeriodicalIF":2.6,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12063351/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143992597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptomic profiling in canine B-cell lymphoma supports a synergistic effect of BTK and PI3K inhibitors.","authors":"Xenia Lainscsek, Weibo Kong, Barbara C Rütgen, Julia Beck, Bertram Brenig, Ingo Nolte, Hugo Murua Escobar, Leila Taher","doi":"10.3389/fvets.2025.1577028","DOIUrl":"https://doi.org/10.3389/fvets.2025.1577028","url":null,"abstract":"<p><strong>Introduction: </strong>B-cell receptor (BCR) signaling has revealed itself as a critical pathway in the pathogenesis of B-cell lymphoma. Within this pathway, the inhibition of Bruton's tyrosine kinase (BTK) or Phosphoinositide 3-kinases (PI3Ks) alone presents encouraging efficacy in the treatment of certain both canine and human hematological malignancies.</p><p><strong>Methods: </strong>Here we characterized the effects of the BTK inhibitor Ibrutinib and the PI3K inhibitor AS-605240 as single and combined agents in the canine pre-clinical diffuse large B cell lymphoma (DLBCL) model CLBL-1 by assaying cell proliferation and metabolic activity, and performing RNA-seq to measure gene expression changes.</p><p><strong>Results: </strong>We found 2,336 differentially expressed genes (DEGs) across all treatment types and time points relative to the control. The largest number of DEGs were induced by the combination of Ibrutinib and AS-605240. These genes were involved in adaptive immune response, leukotriene D4 metabolic and terms related to regulation of GTP and GTPase mediated signal transduction. Weighted gene co-expression network analysis (WGCNA) detected nine gene modules, five of which were associated with treatment response. Eighteen-percent of genes within these modules were also differentially expressed. Notably, we observed one module that was exclusively associated with the combined treatment whose gene members were related to cellular metabolism, homeostasis signaling, and protein synthesis and regulation.</p><p><strong>Conclusion: </strong>Narrowing in on highly connected genes of modules associated with treatment response with large fold changes across treatments which play roles in the main targeted pathways identified <i>PAG1, PRKAR2A, ACACA, FOS</i>, and <i>PRKCA</i> as potential primary candidates of the synergistic treatment effect.</p>","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":"12 ","pages":"1577028"},"PeriodicalIF":2.6,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12063356/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143985189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary supplementation with antibacterial peptide microcin J25 improved antioxidant capacity and intestinal health of pigeons.","authors":"Heng Cao, Yinglin Lu, Xingyu Zhang, Fan Li, Ming Li, Jing Zhou, Huiting He, Qing Ma, Minli Yu","doi":"10.3389/fvets.2025.1550776","DOIUrl":"https://doi.org/10.3389/fvets.2025.1550776","url":null,"abstract":"<p><strong>Introduction: </strong>The present study aimed to evaluate the potential of antimicrobial peptide microcin J25 (MccJ25) as a feed additive for pigeons.</p><p><strong>Methods: </strong>A total of 28-day-old pigeons were allocated to four groups and fed a basal diet (CON) or a basal diet supplemented with 100, 200, or 300 mg/kg MccJ25 (ABP100, ABP200, and ABP300) for 8 weeks.</p><p><strong>Results: </strong>Dietary MccJ25 supplementation significantly improved survival rates in the ABP200 group compared to the control (<i>p</i> < 0.05). Serum analysis revealed that ABP200 and ABP300 groups exhibited increased levels of total protein (TP), globulin (GLB), immunoglobulin A (IgA), and immunoglobulin G (IgG), alongside reduced aspartate aminotransferase (AST), alanine aminotransferase (ALT), total cholesterol (TC), and triglyceride (TG) concentrations (<i>p</i> < 0.05). Furthermore, MccJ25 supplementation enhanced duodenal maltase and trypsin activity (<i>p</i> < 0.05) and upregulated intestinal and hepatic antioxidant capacity, as evidenced by elevated glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) activity (<i>p</i> < 0.05). Intestinal morphology improvements were observed in the jejunum and ileum, with increased villus height-to-crypt depth ratios (VH/CD) (<i>p</i> < 0.05). Additionally, ABP200 and ABP300 groups demonstrated significant upregulation of intestinal barrier-related genes occludin (<i>OCLN</i>), claudin 1 (<i>CLDN1</i>), zonula occluden protein 1 (<i>ZO1</i>), mucin 2 (<i>MUC2</i>), superoxide dismutase 1, 2 (<i>SOD1, 2</i>), and catalase (<i>CAT</i>) in duodenum (<i>p</i> < 0.05).</p><p><strong>Discussion: </strong>These findings indicate that MccJ25 supplementation improves systemic metabolism, enhances antioxidant defenses, strengthens intestinal barrier integrity, and ultimately promotes pigeon health and survival. This study supports the application of MccJ25 as a functional feed additive in poultry production.</p>","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":"12 ","pages":"1550776"},"PeriodicalIF":2.6,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12061914/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143998889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Treatment outcomes of dogs with transitional cell carcinoma.","authors":"Ka To Chu, Omid Nekouei, Antonio Giuliano","doi":"10.3389/fvets.2025.1486786","DOIUrl":"https://doi.org/10.3389/fvets.2025.1486786","url":null,"abstract":"<p><p>Transitional cell carcinoma (TCC) is the most prevalent cancer of the urinary tract in dogs. The prognosis is often poor, and the optimal standard treatment has not been established. The objectives of this study were to (1) describe the clinical outcomes of dogs with TCC, and (2) determine the potential effects of tumor locations and treatment modalities on the survival times of patients. Electronic records of client-owned dogs with TCC treated with different modalities in a large veterinary hospital in Hong Kong (2005-2024) were evaluated. Of 84 confirmed cases included in the study, 49 (58.3%) died or were euthanized due to TCC. Tumors were located in the bladder neck or trigone region (41), apex (26), prostate (10), and urethra (7). Metastases were detected in 10 patients (12%) at diagnosis, including 4 peripheral lymph nodes, 4 lungs, and 2 in the lumbar spine. Of 84 cases, 4 (4.8%) did not receive any treatments, 14 (16.7%) underwent surgery, 25 (29.7%) received metronomic chemotherapy with chlorambucil with/without methotrexate, 27 (32.1%) received COX-2 inhibitors alone, and 14 (16.7%) received conventional chemotherapy, of which, 5 were later switched to metronomic chemotherapy. The overall median survival time was 233 days. There was no statistically significant difference in patients' survival between tumor locations (<i>p</i> > 0.05), aside from tumors involving the prostate that had the shortest MST (88 days). Metronomic chemotherapy led to a significantly longer survival time (median of 303 days) than the other treatment groups (<i>p</i> < 0.05), with the lowest incidence of adverse events. Metronomic chemotherapy using chlorambucil was well-tolerated and can be considered as a single modality treatment or as adjunctive therapy to conventional chemotherapy in dogs with TCC.</p>","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":"12 ","pages":"1486786"},"PeriodicalIF":2.6,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12063355/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143988795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptomic signatures in response to antioxidants supplementation in Korean cattle beef, <i>Hanwoo</i>: a 7-month feeding study.","authors":"Kangwook Lee, La Yoon Choi, Jun Sang Ahn, Jae Yong Song, Joong Kook Park, Suk Jun Yun, Jeong Heon Lee, Eui-Cheol Shin, Soo-Jin Yeom, Jiangchao Zhao, Tae Jin Cho, Nam Su Oh, Jeong-Oh Shin, Dahye Kim, Tae Gyun Kim, Hyung Taek Cho, Hyo Ri Shin, Young Jun Kim, Jae Kyeom Kim","doi":"10.3389/fvets.2025.1546248","DOIUrl":"https://doi.org/10.3389/fvets.2025.1546248","url":null,"abstract":"<p><strong>Introduction: </strong>The present study investigated the effects of antioxidant supplementation on the transcriptomic profiles of <i>Hanwoo</i> cattle during a 7-month feeding trial.</p><p><strong>Methods: </strong>Twelve castrated <i>Hanwoo</i> cattle were randomly assigned to two groups: a control group (CON) and a group supplemented with antioxidants (FEED), consisting of vitamin C, vitamin E, and selenium. Growth performance and carcass traits were evaluated, and liver transcriptomic changes were assessed using RNA sequencing.</p><p><strong>Results and discussion: </strong>While no significant differences were observed in phenotypic traits such as weight gain and feed conversion ratio, transcriptomic analysis identified 641 differentially expressed genes between the CON and FEED groups. Functional enrichment analysis revealed that differentially expressed genes were mainly associated with transcription regulation, pseudouridine synthesis, and mitochondrial function. These findings suggest that antioxidant supplementation elicits significant molecular changes in the liver, particularly affecting transcriptional activity and mitochondrial processes, even in the absence of detectable phenotypic differences.</p>","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":"12 ","pages":"1546248"},"PeriodicalIF":2.6,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12061023/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144063617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Simultaneous detection and partial molecular characterization of five RNA viruses associated with enteric disease in chickens: chicken astrovirus, avian nephritis virus, infectious bronchitis virus, avian rotavirus a and avian orthoreovirus, via multiplex RT-qPCR.","authors":"Anthony Loor-Giler, Claire Muslin, Silvana Santander-Parra, Dayana Coello, Marcela Robayo-Chico, Antonio Piantino Ferreira, Luis Nunez","doi":"10.3389/fvets.2025.1536420","DOIUrl":"https://doi.org/10.3389/fvets.2025.1536420","url":null,"abstract":"<p><p>In the poultry industry, intestinal diseases can lead to significant economic losses due to diarrhea, weight loss and mortality, often linked to viral infections. Chicken astrovirus (CAstV), avian nephritis virus (ANV), infection bronchitis virus (IBV), avian rotavirus A (AvRVA) and avian orthoreovirus (ARV) are key pathogens on this disease including feed malabsorption and runting-stunting syndrome (RSS). This study proposes a multiplex RT-qPCR assay for the simultaneous detection of these five viruses in chickens with enteritis in Ecuador. Primers and hydrolysis probes were designed for the five viruses, along with a synthetic gBlock as a positive control. The method was evaluated for sensitivity, repeatability, and specificity, and 200 jejunal samples were tested. Genome regions of each virus were sequenced, and a phylogenetic analysis confirmed their presence in the samples. The optimized RT-qPCR assay showed efficiency between 98.8-105.9%, with a detection limit of 1 copy/μL. It specifically amplified the five target viruses without cross-reactivity. Among 200 chickens tested, 97% were positive for at least one virus, with ANV (89%) and CAstV (53%) being the most prevalent. Coinfections were common, especially between CAstV and ANV, with three samples positive for all viruses. Sequencing and phylogenetic analysis confirmed the circulation of multiple strains in chickens with enteric disease in Ecuador. This study describes a multiplex RT-qPCR assay for detecting key enteric viruses in Ecuadorian poultry highlighting the high prevalence of astroviruses, emphasizing the impact of coinfections, its possible role in the disease and the importance of improving disease control strategies.</p>","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":"12 ","pages":"1536420"},"PeriodicalIF":2.6,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12058722/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144003183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dose-response study of a fenugreek-based antibiotic alternative in Bábolna Tetra-SL chicks (1-42 days old) with mixed bacterial infections.","authors":"Ádám Kerek, Ábel Szabó, Péter Ferenc Dobra, Krisztina Bárdos, Bettina Paszerbovics, Zsófia Bata, Viviána Molnár-Nagy, Ákos Jerzsele, László Ózsvári","doi":"10.3389/fvets.2025.1570387","DOIUrl":"https://doi.org/10.3389/fvets.2025.1570387","url":null,"abstract":"<p><strong>Introduction: </strong>Combating antimicrobial resistance is one of the most pressing public health challenges of our time. The rapid spread of resistant, zoonotic bacterial strains in livestock farming is increasingly raising concerns about the need to reduce antibiotic use. Because of this, there is an urgent need for safe and effective alternatives in animal husbandry.</p><p><strong>Methods: </strong>This study aimed to perform an <i>in vivo</i> the dose-response analysis of fenugreek (<i>Trigonella foenum-graecum</i>), as a plant-based antibiotic alternative feed supplement in Bábolna Tetra-SL chicks (1-42 days old) with a 1:1 sex ratio. A total of 270 chicks were randomly assigned to 18 groups (15 birds per group) and subjected to six different treatment groups in three replicates: fenugreek at 1×, 10×, and 100× doses, an antibiotic-treated group (enrofloxacin), a positive control group (infection only), and a negative control group (no infection or treatment). The infection was induced using mixed <i>Salmonella Enteritidis</i> and <i>Escherichia coli</i>, administered via gavage on days 3 and 4 of life. The birds were monitored for clinical symptoms, body weight, feed intake, and <i>Salmonella</i> shedding through cloacal swab samples. Statistical analyses included mixed-effect logistic regression for mortality, mixed-effect linear models for weight gain, two-way ANOVA for feed efficiency, and random effects continuation ratio models for <i>Salmonella</i> isolation.</p><p><strong>Results: </strong>Significant interactions for Group:Day and Sex:Day in weight gain were identified (<i>p</i> < 0.0001 for both). Additionally, the 1 × dose group showed significantly reduced <i>Salmonella</i> shedding compared to the positive control group on day 33 (<i>p</i> = 0.0031). The low-dose group (1×) demonstrated the most promising results, showing a 63% reduction in <i>Salmonella</i> shedding on day 10 and 31% on day 17. This group exhibited the fewest clinical symptoms, no diarrhea, and the lowest individual and specific feed intake up to day 24.</p><p><strong>Discussion: </strong>The findings of this study suggest that low-dose fenugreek supplementation could be a viable strategy for reducing <i>Salmonella</i> shedding in poultry, potentially contributing to reduced antibiotic use in poultry farming and thus playing a role in the global effort to combat antimicrobial resistance. Future research will involve large-scale industrial trials and next-generation sequencing to evaluate the additive's impact on gut microbiota composition.</p>","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":"12 ","pages":"1570387"},"PeriodicalIF":2.6,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12058679/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143982902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Global pharmacovigilance reporting of the first monoclonal antibody for canine osteoarthritis: a case study with bedinvetmab (Librela™).","authors":"Beatriz P Monteiro, Anthony Simon, Oliver Knesl, Kristen Mandello, Steven Nederveld, Natasha J Olby, John F Innes, B Duncan X Lascelles","doi":"10.3389/fvets.2025.1558222","DOIUrl":"https://doi.org/10.3389/fvets.2025.1558222","url":null,"abstract":"<p><strong>Introduction: </strong>Continuous product monitoring post approval builds on the knowledge gained during clinical studies to aid in understanding a product's safety and efficacy profile. Pharmacovigilance reporting of a medicinal product might be influenced by several factors including duration in the market, geographical region and veterinary practices. The goals of this report are to present the global data accrued for bedinvetmab, the first monoclonal antibody for canine osteoarthritis, and to explore reporting patterns globally and across major markets.</p><p><strong>Methods: </strong>Adverse event reports from the Zoetis Global Pharmacovigilance database (from first introduction on 01 February 2021 through 30 June 2024) were collected irrespective of suspected causality or off-label use. Each adverse event was coded using the Veterinary Dictionary for Drug Related Affairs (VeDDRA) terminology. The top 20 most reported VeDDRA terms were identified. Countries were ranked by number of doses distributed and frequency of adverse events.</p><p><strong>Results: </strong>Globally, 18,102,535 doses of bedinvetmab were sold during the study period with a total of 17,162 adverse events reported in dogs (9.48 events/10,000 treated animals (doses)). Eight clinical signs were considered rare (1-10 events/10,000 treated animals (doses)) with lack of efficacy having the highest rate (1.70) followed by polydipsia, ataxia, polyuria/pollakiuria, anorexia, lethargy, death, and emesis. All other clinical signs were considered very rare (< 1 event/10,000 treated animals (doses)). Median (interquartile range) of dogs' age and body weight were 12 (10-13) years and 26 (16-34.6) kg, respectively. The top eight countries by market size were United States (US), United Kingdom (UK), Germany, Spain, France, Italy, Canada, and Australia; from these, the top five by frequency of adverse events were Canada, US, UK, Australia and Germany. The most reported adverse events following bedinvetmab are considered rare or very rare.</p><p><strong>Discussion: </strong>The reported clinical signs generally aligned with expected adverse events or were anticipated within the population receiving bedinvetmab. Reporting rates and patterns in general and for specific VeDDRA terms greatly varied between countries and were not related to market size. Most dogs for which adverse events were reported were considered older and in fair clinical condition. Reporting to pharmacovigilance contributes to the understanding of the safety profile of a medicinal product.</p>","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":"12 ","pages":"1558222"},"PeriodicalIF":2.6,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12061024/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143965343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Case Report: A case study on the relationship between obesity and estrus in female captive panda.","authors":"Qiang Zhou, Bo Luo, Bo Yang, Desheng Li, Rongping Wei, Chunyu Xie, Jinhua Yu, Xiangqian Meng, Jianbin Cheng, Ming He, Liu Yang","doi":"10.3389/fvets.2025.1552754","DOIUrl":"https://doi.org/10.3389/fvets.2025.1552754","url":null,"abstract":"<p><p>This study investigated a case of abnormal estrus in a captive adult female giant panda (<i>Ailuropoda melanoleuca</i>) (X#, 130 kg) in 2024 to explore the possible relationship between overweight or obesity and estrus behavior in female giant pandas. Behavioral observations and urinary estrogen conjugate/creatinine (EC/CR) measurements revealed a significantly attenuated hormonal profile (peak EC/CR: 34.1 ng/mg vs. normal range 80-150 ng/mg) alongside muted receptivity (e.g., absent tail-lifting). Comparative analysis with historical cases of abnormal estrus (panda 2#: obesity-linked low EC/CR; panda 1#: normal EC/CR with behavioral anomalies) implicated obesity as a driver of reproductive impairment. And several actionable interventions were proposed: bamboo-based dietary reform, structured exercise protocols, and gut microbiota monitoring. This case underscores obesity as a modifiable risk factor in captive panda reproduction, urging integration of metabolic health into breeding management.</p>","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":"12 ","pages":"1552754"},"PeriodicalIF":2.6,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12058823/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144004090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}