Frontiers in Veterinary Science最新文献

{"title":"Pathology and host-pathogen interactions in a golden Syrian hamster model of Nipah virus infection.","authors":"Inés Ruedas-Torres, Stephen Findlay-Wilson, Emma Kennedy, Stuart Dowall, Francisco Javier Salguero","doi":"10.3389/fvets.2025.1518358","DOIUrl":"10.3389/fvets.2025.1518358","url":null,"abstract":"<p><p>Nipah virus (NiV) is recognized as one of the key pathogens with pandemic potential. We have recently established a NiV hamster model, which reproduces a highly similar disease to that observed in human cases, including respiratory and neurological signs and lesions. The aims of this study were to describe the microscopic lesions observed in the golden Syrian hamster model after intranasal (IN) and intraperitoneal (IP) inoculation with different doses of the Malaysian strain of NiV; to describe in depth the cell composition of the pulmonary and the brain lesions and the expression of proinflammatory cytokines <i>in-situ</i> using a combination of histopathological techniques including immunohistochemistry (IHC) and <i>in-situ</i> hybridisation (ISH) via RNAscope technique. We also developed a multiplex IHC which will allow us to study the interaction of the virus with cell populations in the lung and brain in future studies. For this, we selected 28 lung and brain formalin-fixed paraffin-embedded (FFPE) samples from previous experiments performed by our research group. Histopathology revealed severe pulmonary broncho-interstitial pneumonia, mainly in animals inoculated via the IN route, accompanied by a strong acute inflammatory response (Iba1⁺ cells) and high levels of NiV RNA. Upregulation of proinflammatory cytokines (IL-6 and TNF) was also observed by ISH RNAscope technique in these animals. Neurological lesions, consisting of perivascular cuffing and meningitis, were observed mainly in animals inoculated via IP route. IHC results showed astrocytosis (GFAP⁺) and microgliosis (Iba1⁺) in the brain of these animals, together with mild levels of IL6 and TNF mRNA. These results have helped us to characterize the host-pathogen interaction in the golden Syrian hamster animal model of NiV infection that is being currently used in preclinical testing of antiviral and vaccine strategies. Techniques used in this study could be applied to the development and application of golden Syrian hamster models of other infections by henipaviruses, including Hendra virus (HeV), and other high consequence priority pathogens.</p>","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":"12 ","pages":"1518358"},"PeriodicalIF":2.6,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11926554/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143691715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editorial: Estimating non-monetary societal burden of livestock disease management.","authors":"Chisoni Mumba, Guillaume Lhermie, Karl M Rich","doi":"10.3389/fvets.2025.1571267","DOIUrl":"10.3389/fvets.2025.1571267","url":null,"abstract":"","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":"12 ","pages":"1571267"},"PeriodicalIF":2.6,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11927214/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143691679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inhibiting ferroptosis mitigates sheep sperm freezing damage.","authors":"Erhan Hai, Boyuan Li, Yukun Song, Jian Zhang, Jiaxin Zhang","doi":"10.3389/fvets.2025.1526474","DOIUrl":"10.3389/fvets.2025.1526474","url":null,"abstract":"<p><strong>Objectives: </strong>To evaluate the roles of apoptosis and ferroptosis in cryopreservation-induced damage to sheep sperm, with a focus on assessing the effectiveness of inhibitors targeting these pathways.</p><p><strong>Methods: </strong>Initial analysis compared the expression of apoptotic marker Cleaved-caspase3 (CL-caspase3) and ferroptotic marker Transferrin receptor (TFRC) between fresh and cryopreserved sheep sperm. Elevated CL-caspase3 expression and sustained high TFRC expression post-cryopreservation suggested concurrent occurrence of apoptosis and ferroptosis. Consequently, the study employed Deferoxamine Mesylate (DFO), ferrostatin-1 (Fer-1), liproxstatin-1 (Lip-1), and the apoptosis inhibitor Z-VAD-FMK (Z-VAD) at concentrations ranging from 0 to 10 μM. Post-thaw assessments encompassed plasma membrane integrity, acrosome integrity, and ferroptosis biomarkers. Additional experiments were conducted to measure the expression of GPX4, a key regulator of ferroptosis.</p><p><strong>Results: </strong>Optimal concentrations (2 μM for DFO, Fer-1, and Lip-1; 5 μM for Z-VAD) significantly improved sperm motility and membrane integrity. Among these, Fer-1 demonstrated the greatest efficacy, reducing reactive oxygen species (ROS), lipid peroxidation, and Fe²⁺ levels. Z-VAD primarily decreased ROS but was less potent than ferroptosis inhibitors. Notably, Glutathione Peroxidase 4 (GPX4) expression was reduced post-cryopreservation, while Fer-1 supplementation restored its levels to those comparable with fresh sperm.</p><p><strong>Conclusion: </strong>Both apoptosis and ferroptosis play critical roles in sheep sperm cryopreservation. Fer-1 effectively enhanced cryopreservation outcomes by inhibiting ferroptosis, as evidenced by the restoration of GPX4 expression and improvement in sperm quality indicators. These findings highlight ferroptosis inhibition as a promising strategy for preserving genetic material, with implications for animal breeding and biodiversity conservation.</p>","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":"12 ","pages":"1526474"},"PeriodicalIF":2.6,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11926617/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143691702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of four traditional Chinese medicine monomers on mechanical barrier damage and inflammation response of IPEC-J2 cells caused by soybean 7S globulin.","authors":"Youtian Deng, Xiaoli Wu, Yingying Wan, Junliang Deng, Huidan Deng","doi":"10.3389/fvets.2025.1548866","DOIUrl":"10.3389/fvets.2025.1548866","url":null,"abstract":"<p><strong>Introduction: </strong>The soybean 7S globulin is a major allergen responsible for diarrhea in weaned piglets, leading to significant economic loss in the pig breeding industry. Therefore, there is an urgent need to find effective and safe therapeutic agents to prevent and treat diarrhea caused by soybean 7S globulin. Given the global trend toward \"antibiotic alternatives,\" traditional Chinese medicines (TCMs) offer a promising approach for the prevention and control of animal diseases.</p><p><strong>Methods: </strong>In this study, four TCM monomers (curcumin, eleutheroside E, saponin B4, and forsythia A) were evaluated for their protective and therapeutic effects on intestinal epithelial cells (IPEC-J2) damaged by soybean 7S globulin. The CCK8 assay, western blot assay, Elisa assay, and PCR assay were used in this study.</p><p><strong>Results: </strong>The results demonstrated that curcumin at concentrations of 0.02, 0.04, and 0.08 μg/mL, eleutheroside E at 25, and 50 μg/mL, saponin B4 at 12.5, 25, and 50 μg/mL, and forsythia A at 20, and 40 μg/mL had significant ameliorative effects on cell viability, permeability, and integrity. Furthermore, the TCM monomers alleviated the inflammatory response, reduced the disruption of tight junctions, and improved the cellular mechanical barrier. These protective effects were likely mediated through the inhibition of the Rho/ROCK signaling pathway, characterized by down regulation of RhoA, ROCK1, ROCK2, and MLKC expression.</p><p><strong>Discussion: </strong>These findings suggest that the four TCM monomers have the potential to treat diarrhea of weaned piglets caused by soybean protein.</p>","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":"12 ","pages":"1548866"},"PeriodicalIF":2.6,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11925905/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143691687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Is extra virgin olive oil a promising remedy for reducing the impact of postmenopausal osteoporosis? An experimental study.","authors":"El-Sayed El-Shafaey, Eman Ali, Magda Elkomy, Mohamed Abdo Rizk, Saleh Altuwaijri, Saleh Albarrak","doi":"10.3389/fvets.2025.1555779","DOIUrl":"10.3389/fvets.2025.1555779","url":null,"abstract":"<p><strong>Introduction: </strong>Osteoporosis, particularly postmenopausal osteoporosis, is a significant global health challenge with limited treatment options due to severe side effects associated with the long-term use of conventional therapies. Therefore, this study aims to provide a potentially novel therapeutic approach by examining olive oil's effects on bone mineral density (BMD), biochemical markers, biomechanical properties, and histopathological changes in an ovariectomized (OVX) rat model.</p><p><strong>Methods: </strong>In this study, Twenty-four 6-month-old female Wistar rats were randomly allocated into four equal groups (<i>n</i> = 6 rats, for each group): control group, rats given 1mL/100g olive oil, ovariectomized rats (OVX-group), and OVX rats treated with olive oil. The femoral bone mineral density (BMD), biochemical parameters, biomechanical properties, and histopathological features were studied.</p><p><strong>Results: </strong>After 3 months of extra virgin olive oil treatment, there were significant improvements in the different estimated parameters. This was demonstrated by preventing the changes in bone remodeling and BMD, improving the hormonal changes, oxidant/antioxidant imbalance, and abnormal levels of pro-inflammatory cytokines associated with OVX-induced osteoporosis. In addition, there was a marked improvement in the histological architecture of the cancellous and cortical bone appearance.</p><p><strong>Conclusion: </strong>Olive oil dietary intake effectively reduces the impact of osteoporosis induced by ovariectomy in rats, suggesting a potentially feasible treatment option for postmenopausal osteoporosis that benefits bone architecture without any detrimental side effects on women's health.</p>","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":"12 ","pages":"1555779"},"PeriodicalIF":2.6,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11927216/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143691708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Frustration and its impact on search and rescue canines.","authors":"Sally Dickinson, Erica N Feuerbacher","doi":"10.3389/fvets.2025.1546412","DOIUrl":"10.3389/fvets.2025.1546412","url":null,"abstract":"<p><p>Despite advances in modern technology, dogs remain the primary detection tool in search and rescue (SAR) missions, locating missing persons across diverse and dynamic environments, including wilderness, avalanche zones, water, and disaster areas. Their exceptional olfactory abilities, combined with their capacity to process complex discrimination tasks and adapt to varied environmental stimuli, make them uniquely suited for this work. However, SAR operations can be both physically and psychologically demanding, requiring sustained focus, endurance, and consistent performance under stressful conditions. Frustration, a form of psychological stress, arises when a dog encounters blocked access to a goal or when an expectation is violated, triggering physiological and behavioral changes that may impact performance. This study investigated the physiological and behavioral responses of SAR dogs to two distinct stress conditions: psychological stress induced by frustration and physiological stress induced by moderate exercise. We measured heart rate variability as an indicator of autonomic nervous system response to stress and analyzed search task performance to assess how frustration and exercise affected the dogs' latency and accuracy in executing their trained final response to the target odor. Our results revealed significant decreases in heart rate variability following frustration and increased latency in the search task, suggesting that frustration had a more pronounced impact on the dogs' physiological state and performance compared to exercise. By examining the effects of psychological and physiological stress, this study contributes to a deeper understanding of how different stressors influence SAR dog performance and welfare. These findings provide valuable insights for optimizing training methodologies and operational preparedness, ensuring both the effectiveness and well-being of SAR dogs in the field.</p>","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":"12 ","pages":"1546412"},"PeriodicalIF":2.6,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11926599/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143691697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-heat stress lick block supplementation alleviated the detrimental effects of heat stress on dairy cows.","authors":"Hongwei Duan, Jiyou Zhang, Na Li, Liuping Chen, Danhong Chen, Hang Yang, Qiuxia Dai, Junshi Shen, Shengyong Mao","doi":"10.3389/fvets.2025.1562964","DOIUrl":"10.3389/fvets.2025.1562964","url":null,"abstract":"<p><strong>Introduction: </strong>Heat stress poses a significant challenge to the development of dairy industry, affecting cows' well-being and overall productivity, leading to substantial economic losses. In this study, the impact of a specifically formulated anti-heat stress lick block supplement on milk production, milk quality, feed intake, rectal temperature, respiratory rate, and rumen fermentation in cows exposed to heat-stress was evaluated.</p><p><strong>Methods: </strong>Twenty-four healthy Holstein lactating dairy cows were divided into two blocks based on milk yield (low and high), Parity (2-3 parity), and lactation days (114 ± 8 d). The cows in each block were randomly assigned to either a control group without lick block supplementation or a treatment group receiving lick block. The trial lasted for 6 weeks, including a 2-week adaptation phase followed by 4 weeks of feeding treatment.</p><p><strong>Results: </strong>Heat stress levels varied from severe (THI > 88) to moderate heat stress (THI > 80) in the first 2 weeks, gradually decreasing to mild heat stress (THI > 72) in the following weeks. With the decrease in heat stress, dry matter intake (DMI) and milk production increased (Week: <i>p</i> < 0.05), the rectal temperature and respiratory rate of cows decreased (Week: <i>p</i> < 0.05). Lick block supplementation tended to increase DMI (<i>p</i> = 0.09), and improved milk yield (<i>p</i> < 0.05) without affecting (<i>p</i> > 0.05) milk composition, leading to increased milk yields of fat, protein, and lactose (<i>p</i> < 0.05). Although the overall rectal temperature of cows in the lick block group did not differ from the control group (<i>p</i> > 0.05), the respiratory rate of cows in the lick block group significantly decreased (<i>p</i> < 0.05) in the second and third weeks. Supplementation with the lick block increased (<i>p</i> < 0.05) rumen pH and decreased (<i>p</i> < 0.05) NH₃-N and propionate concentrations in dairy cows, and tended to lower the acetate-to-propionate ratio (<i>p</i> = 0.07), total VFA concentration (<i>p</i> = 0.07), and butyrate concentration (<i>p</i> = 0.09).</p><p><strong>Conclusion: </strong>Supplementation of anti-heat stress lick block alleviated the detrimental effects of heat stress on dairy cows within a certain range of temperature and humidity.</p>","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":"12 ","pages":"1562964"},"PeriodicalIF":2.6,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11927217/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143692063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editorial: Use of cannabis derivatives in veterinary medicine.","authors":"Robin Temmerman","doi":"10.3389/fvets.2025.1539422","DOIUrl":"10.3389/fvets.2025.1539422","url":null,"abstract":"","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":"12 ","pages":"1539422"},"PeriodicalIF":2.6,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11926136/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143691682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Herbal formula alleviates heat stress by improving physiological and biochemical attributes and modulating the rumen microbiome in dairy cows.","authors":"Xiaofang Wang, Yawen Wang, Man Feng, Jiefeng Li, Ze Liu, Le Fu, Ning Zhang, Huaying Zhang, Jianhua Qin","doi":"10.3389/fvets.2025.1558856","DOIUrl":"10.3389/fvets.2025.1558856","url":null,"abstract":"<p><p>Heat stress significantly impacts dairy cow productivity, health, and welfare. This study evaluated a self-developed herbal formula as a dietary intervention to mitigate heat stress. A total of 198 lactating cows were divided into two groups: a Control group receiving standard total mixed rations and a Herbs group supplemented with herbal formula for 60 days. Various parameters were assessed, including milk yield and composition, antioxidant capacity, immune responses, stress-related gene expression, and rumen microbial composition. Compared to the Control group, cows in the Herbs group showed improved feed intake, milk yield and quality, rumination frequency, and enhanced antioxidant activity and immune response. Rumen microbiome analysis revealed a reduced relative abundance of Proteobacteria and <i>Ochrobactrum</i> in the Herbs group, along with an enrichment of beneficial genera such as <i>Lachnospira</i>. Functional predictions indicated that the Herbs group exhibited enhanced glycolysis/gluconeogenesis, pyruvate metabolism, and starch and sucrose metabolism, reflecting improved fermentation efficiency and energy utilization. In conclusion, the herbal formula improved physiological and biochemical attributes, boosted antioxidant and immune responses, and modulated the rumen microbiome, contributing to the alleviation of heat stress in dairy cows. These findings highlight its potential as a natural dietary strategy to support dairy cow health and productivity under heat stress conditions.</p>","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":"12 ","pages":"1558856"},"PeriodicalIF":2.6,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11925914/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143691701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The neurobiological basis of emotions and their connection to facial expressions in non-human mammals: insights in nonverbal communication.","authors":"Daniel Mota-Rojas, Alexandra L Whittaker, Cécile Bienboire-Frosini, Jhon Buenhombre, Patricia Mora-Medina, Adriana Domínguez-Oliva, Julio Martínez-Burnes, Ismael Hernández-Avalos, Adriana Olmos-Hernández, Antonio Verduzco-Mendoza, Alejandro Casas-Alvarado, Karina Lezama-García, Temple Grandin","doi":"10.3389/fvets.2025.1541615","DOIUrl":"10.3389/fvets.2025.1541615","url":null,"abstract":"<p><p>Recognizing that nonhuman animals are sentient beings has increased interest in studying their emotional state. Similar to humans, research has shown that some nonhuman mammals can modify facial expressions by contraction/relaxation of facial muscles according to their affective state. From a neurophysiological perspective, emotions are processed in several brain structures, mainly from the limbic system, such as the hypothalamus, hypophysis, hippocampus, prefrontal cortex, and amygdala. The converged pathways between the amygdala, the motor cortex, and its projections to the facial nerve control the movement of facial or mimetic muscles. Thus, facial expression is suggested to reflect the internal emotional state and could serve as an essential mode of nonverbal communication in mammals. In humans, the Facial Action Coding System (FACS) is a method that objectively analyzes facial movements using an anatomical base. In veterinary medicine, AnimalFACS is an adaptation of this system to eight animal species, including domestic animals (dogs, cats, and horses) and nonhuman primates (chimpanzees, orangutans, gibbons, macaques, and common marmosets). Considering these coded facial movements, current research aims to associate certain facial expressions with the animals' emotional states and affective contexts. Thus, this review aims to discuss recent findings associated with the neurobiology of emotions and facial expressions in non-human mammals, using AnimalFACS to understand nonverbal communication. Characterizing each facial expression according to different contexts might help identify if the animal is expressing a positive or negative emotional response to the event, which can improve nonverbal human-animal communication.</p>","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":"12 ","pages":"1541615"},"PeriodicalIF":2.6,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11926555/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143691723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}