Glycoconjugate Journal最新文献_第8页

Characterization and antioxidant activities of glycosaminoglycans from dried leech. 干水蛭糖胺聚糖的表征及其抗氧化活性。

IF 3 4区生物学

Glycoconjugate Journal Pub Date : 2023-04-01 DOI: 10.1007/s10719-023-10105-y

Tao Shen, Shangteng Wang, Quntao Liang, Joshua S Sharp, Zheng Wei

{"title":"Characterization and antioxidant activities of glycosaminoglycans from dried leech.","authors":"Tao Shen, Shangteng Wang, Quntao Liang, Joshua S Sharp, Zheng Wei","doi":"10.1007/s10719-023-10105-y","DOIUrl":"https://doi.org/10.1007/s10719-023-10105-y","url":null,"abstract":"Dried leech (Whitmania pigra whitman) has been widely used as a traditional animal-based Chinese medicine. Dried leech extracts have been reported to have various biological activities that are often associated with mammalian glycosaminoglycans. However, their presence and possible structural characteristics within dried leech were previously unknown. In this study, glycosaminoglycans were isolated from dried leech for the first time and their structures were analyzed by the combination of Fourier-transform infrared spectroscopy, liquid chromatography-ion trap/time-of-flight mass spectrometry and polyacrylamide gel electrophoresis. Heparan sulfate and chondroitin sulfate/dermatan sulfate were detected in dried leech with varied disaccharide compositions and possess a heterogeneous structure. Heparan sulfate species possess an equal amount of total 2-O-sulfated, N-sulfated and acetylated disaccharides, while chondroitin sulfate /dermatan sulfate contain high content of 4-O-sulfated disaccharides. Also, the quantitative analysis revealed that the contents of heparan sulfate and chondroitin/dermatan sulfate in dried leech varied significantly, with chondroitin/dermatan sulfate being by far the most abundant. This novel structural information could help clarify the possible involvement of these polysaccharides in the biological activities of the dried leech. Furthermore, leech glycosaminoglycans showed a strong ABTS radical scavenging ability, which suggests the potential of leech polysaccharides for exploitation in the nutraceutical and pharmaceutical industries.","PeriodicalId":12762,"journal":{"name":"Glycoconjugate Journal","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9272888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sialoadhesin (CD169/Siglec-1) is an extended molecule that escapes inhibitory cis-interactions and synergizes with other macrophage receptors to promote phagocytosis. 唾液粘附素(CD169/ siglec1)是一种扩展的分子，可以逃避抑制性顺式相互作用，并与其他巨噬细胞受体协同作用，促进吞噬。

IF 3 4区生物学

Glycoconjugate Journal Pub Date : 2023-04-01 DOI: 10.1007/s10719-022-10097-1

Mariliis Klaas, Stuart Dubock, David J P Ferguson, Paul R Crocker

{"title":"Sialoadhesin (CD169/Siglec-1) is an extended molecule that escapes inhibitory cis-interactions and synergizes with other macrophage receptors to promote phagocytosis.","authors":"Mariliis Klaas, Stuart Dubock, David J P Ferguson, Paul R Crocker","doi":"10.1007/s10719-022-10097-1","DOIUrl":"https://doi.org/10.1007/s10719-022-10097-1","url":null,"abstract":"Sialoadhesin (CD169/Siglec-1, Sn) is a macrophage receptor that interacts with sialic acids on both host cells and pathogens. It is a type 1 membrane protein with an unusually large number of 17 extracellular immunoglobulin (Ig)-like domains, made up of an N-terminal V-set domain that binds sialic acid and 16 adjacent C2-set domains. The potential importance of 17 Ig domains in Sn for mediating cellular interactions has not been investigated experimentally. In the present study, Chinese Hamster Ovary (CHO) cells were stably transfected with full-length or truncated forms of Sn. Using human red blood cells (RBC) as a model system, CHO cells expressing truncated forms of Sn with 4 or less Ig domains were unable to bind RBC in comparison to the full-length protein. Immunoelectron microscopy of the CHO cells indicated that full-length Sn extends ~ 33 nm from the plasma membrane compared with ~ 14 nm for a truncated form with 6 N-terminal Ig domains. Co-expresssion of Sn-expressing CHO cells with heavily glycosylated membrane proteins of differing predicted lengths resulted in selective modulation of Sn-dependent binding to RBC and supported the hypothesis that Sn has evolved 17 Ig domains to escape inhibitory cis-interactions. The functional significance of the extended length of Sn was demonstrated in experiments with macrophages showing that Sn synergizes with phagocytic receptors FcR and TIM-4 to strongly promote uptake of IgG-opsonized and eryptotic RBC respectively.","PeriodicalId":12762,"journal":{"name":"Glycoconjugate Journal","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10027830/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9280432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative study, homology modelling and molecular docking with cancer associated glycans of two non-fetuin-binding Tepary bean lectins. 两种非胎盘素结合型泰帕瑞豆凝集素与癌症相关聚糖的比较研究、同源建模和分子对接。

IF 2.7 4区生物学

Glycoconjugate Journal Pub Date : 2023-02-01 Epub Date: 2022-11-17 DOI: 10.1007/s10719-022-10091-7

Iovanna Torres-Arteaga, Alejandro Blanco-Labra, Elizabeth Mendiola-Olaya, Teresa García-Gasca, Cesar Aguirre-Mancilla, Alondra L Ortega-de-Santiago, Mariana Barboza, Carlito B Lebrilla, José Luis Castro-Guillén

{"title":"Comparative study, homology modelling and molecular docking with cancer associated glycans of two non-fetuin-binding Tepary bean lectins.","authors":"Iovanna Torres-Arteaga, Alejandro Blanco-Labra, Elizabeth Mendiola-Olaya, Teresa García-Gasca, Cesar Aguirre-Mancilla, Alondra L Ortega-de-Santiago, Mariana Barboza, Carlito B Lebrilla, José Luis Castro-Guillén","doi":"10.1007/s10719-022-10091-7","DOIUrl":"10.1007/s10719-022-10091-7","url":null,"abstract":"We present the purification and characterization of the two most abundant isoforms of lectins isolated from Tepary bean (Phaseolus acutifolius) seeds, which have been shown to differentially affect the survival of different cancer cells. They were separated by concanavalin A-affinity chromatography. After purification, to release the N-glycans, they were digested with the endoglycosidases PNGase and Glycanase A. Fractions resulted from the hydrolysis products were analyzed to determine their carbohydrate composition. Mass spectrometry data indicated that both isoforms contained high mannose glycans being mannose 6 the most abundant form. Furthermore, based on sequence Ans-X-Ser/Thr, where X is any amino acid except proline, a glycosylation site was determined on asparagine 36. When their metal requirement to preserve their biological activity was determined, the lectins showed differences. While lectin A (LA) agglutination activity was best in the presence of magnesium, lectin B (LB) was best with calcium. Additionally, only LA exhibited affinity to human type-A erythrocytes. Although both lectins showed small differences in their properties, an identical structure-model for both lectins was generated by the homology modelling process. Also, the analysis of ligand binding sites and in silico glycosylation were achieved. Molecular docking with colon adenocarcinoma associated-N-glycans revealed some highly possible interactions and, on the other hand, that N-glycan interaction zones of Tepary bean lectins is not restricted to the carbohydrate binding domain but to an extended part of their surface, which could lead new strategies to explain their biological activity.","PeriodicalId":12762,"journal":{"name":"Glycoconjugate Journal","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10730604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The lectin DrfL inhibits cell migration, adhesion and triggers autophagy-dependent cell death in glioma cells. 凝集素DrfL能抑制胶质瘤细胞的迁移和粘附，并引发依赖自噬的细胞死亡。

IF 2.7 4区生物学

Glycoconjugate Journal Pub Date : 2023-02-01 Epub Date: 2022-12-16 DOI: 10.1007/s10719-022-10095-3

Ingrid A V Wolin, Ana Paula M Nascimento, Rodrigo Seeger, Gabriela G Poluceno, Alfeu Zanotto-Filho, Claudia B Nedel, Carla I Tasca, Sarah Elizabeth Gomes Correia, Messias Vital Oliveira, Vanir Reis Pinto-Junior, Vinicius Jose Silva Osterne, Kyria Santiago Nascimento, Benildo Sousa Cavada, Rodrigo Bainy Leal

{"title":"The lectin DrfL inhibits cell migration, adhesion and triggers autophagy-dependent cell death in glioma cells.","authors":"Ingrid A V Wolin, Ana Paula M Nascimento, Rodrigo Seeger, Gabriela G Poluceno, Alfeu Zanotto-Filho, Claudia B Nedel, Carla I Tasca, Sarah Elizabeth Gomes Correia, Messias Vital Oliveira, Vanir Reis Pinto-Junior, Vinicius Jose Silva Osterne, Kyria Santiago Nascimento, Benildo Sousa Cavada, Rodrigo Bainy Leal","doi":"10.1007/s10719-022-10095-3","DOIUrl":"10.1007/s10719-022-10095-3","url":null,"abstract":"Glioblastoma multiforme (GBM) is the most aggressive type of glioma, displaying atypical glycosylation pattern that may modulate signaling pathways involved in tumorigenesis. Lectins are glycan binding proteins with antitumor properties. The present study was designed to evaluate the antitumor capacity of the Dioclea reflexa lectin (DrfL) on glioma cell cultures. Our results demonstrated that DrfL induced morphological changes and cytotoxic effects in glioma cell cultures of C6, U-87MG and GBM1 cell lines. The action of DrfL was dependent upon interaction with glycans, and required a carbohydrate recognition domain (CRD), and the cytotoxic effect was apparently selective for tumor cells, not altering viability and morphology of primary astrocytes. DrfL inhibited tumor cell migration, adhesion, proliferation and survival, and these effects were accompanied by activation of p38MAPK and JNK (p46/54), along with inhibition of Akt and ERK1/2. DrfL also upregulated pro-apoptotic (BNIP3 and PUMA) and autophagic proteins (Atg5 and LC3 cleavage) in GBM cells. Noteworthy, inhibition of autophagy and caspase-8 were both able to attenuate cell death in GBM cells treated with DrfL. Our results indicate that DrfL cytotoxicity against GBM involves modulation of cell pathways, including MAPKs and Akt, which are associated with autophagy and caspase-8 dependent cell death.","PeriodicalId":12762,"journal":{"name":"Glycoconjugate Journal","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10730297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pseudomonas syringae DC3000 infection increases glucosylated N-glycans in Arabidopsis thaliana. 拟南芥中的丁香假单胞菌 DC3000 感染会增加葡萄糖基化的 N-聚糖。

IF 2.7 4区生物学

Glycoconjugate Journal Pub Date : 2023-02-01 Epub Date: 2022-10-21 DOI: 10.1007/s10719-022-10084-6

Gernot Beihammer, Andrea Romero-Pérez, Daniel Maresch, Rudolf Figl, Réka Mócsai, Clemens Grünwald-Gruber, Friedrich Altmann, Els J M Van Damme, Richard Strasser

{"title":"Pseudomonas syringae DC3000 infection increases glucosylated N-glycans in Arabidopsis thaliana.","authors":"Gernot Beihammer, Andrea Romero-Pérez, Daniel Maresch, Rudolf Figl, Réka Mócsai, Clemens Grünwald-Gruber, Friedrich Altmann, Els J M Van Damme, Richard Strasser","doi":"10.1007/s10719-022-10084-6","DOIUrl":"10.1007/s10719-022-10084-6","url":null,"abstract":"Studying the interaction between the hemibiotrophic bacterium Pseudomonas syringae pv. tomato DC3000 and Arabidopsis thaliana has shed light onto the various forms of mechanisms plants use to defend themselves against pathogen attack. While a lot of emphasis has been put on investigating changes in protein expression in infected plants, only little information is available on the effect infection plays on the plants N-glycan composition. To close this gap in knowledge, total N-glycans were enriched from P. syringae DC3000-infected and mock treated Arabidopsis seedlings and analyzed via MALDI-TOF-MS. Additionally, fluorescently labelled N-glycans were quantified via HPLC-FLD. N-glycans from infected plants were overall less processed and displayed increased amounts of oligomannosidic N-glycans. As multiple peaks for certain oligomannosidic glycoforms were detected upon separation via liquid chromatography, a porous graphitic carbon (PGC)-analysis was conducted to separate individual N-glycan isomers. Indeed, multiple different N-glycan isomers with masses of two N-acetylhexosamine residues plus 8, 9 or 10 hexoses were detected in the infected plants which were absent in the mock controls. Treatment with jack bean α-mannosidase resulted in incomplete removal of hexoses from these N-glycans, indicating the presence of glucose residues. This hints at the accumulation of misfolded glycoproteins in the infected plants, likely because of endoplasmic reticulum (ER) stress. In addition, poly-hexose structures susceptible to α-amylase treatment were found in the DC3000-infected plants, indicating alterations in starch metabolism due to the infection process.","PeriodicalId":12762,"journal":{"name":"Glycoconjugate Journal","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9925501/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10734654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The story of the Sd^a antigen and of its cognate enzyme B4GALNT2: What is new? Sda 抗原及其同源酶 B4GALNT2 的故事：有什么新发现？

IF 2.7 4区生物学

Glycoconjugate Journal Pub Date : 2023-02-01 Epub Date: 2022-10-26 DOI: 10.1007/s10719-022-10089-1

Martina Duca, Nadia Malagolini, Fabio Dall'Olio

{"title":"The story of the Sda antigen and of its cognate enzyme B4GALNT2: What is new?","authors":"Martina Duca, Nadia Malagolini, Fabio Dall'Olio","doi":"10.1007/s10719-022-10089-1","DOIUrl":"10.1007/s10719-022-10089-1","url":null,"abstract":"The structure Siaα2,3(GalNAcβ1,4)Gal- is the epitope of the Sda antigen, which is expressed on the erythrocytes and secretions of the vast majority of Caucasians, carried by N- and O-linked chains of glycoproteins, as well as by glycolipids. Sda is very similar, but not identical, to ganglioside GM2 [Siaα2,3(GalNAcβ1,4)Galβ1,4Glc-Cer]. The Sda synthase β1,4 N-acetylgalactosaminyl transferase 2 (B4GALNT2) exists in a short and a long form, diverging in the aminoterminal domain. The latter has a very long cytoplasmic tail and displays a Golgi- as well as a post-Golgi localization. The biosynthesis of Sda is mutually exclusive with that of the cancer-associated sialyl Lewis antigens, whose structure is Siaα2,3Galβ1,3/4(Fucα1,4/3)GlcNAc-. B4GALNT2 is down-regulated in colon cancer but patients with higher expression survive longer. In experimental systems, B4GALNT2 inhibits colon cancer progression,not only through inhibition of sialyl Lewis antigen biosynthesis. By contrast, in breast cancer B4GALNT2 is associated with malignancy. In colon cancer, the B4GALNT2 gene is regulated by multiple mechanisms, which include miRNA and transcription factor expression, as well as CpG methylation. In addition, Sda/B4GALNT2 regulates the susceptibility to infectious agents, the protection from muscle dystrophy, the activity of immune system in pregnancy and the immune rejection in xenotransplantation.","PeriodicalId":12762,"journal":{"name":"Glycoconjugate Journal","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10730559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Plant lectins as versatile tools to fight coronavirus outbreaks. 植物凝集素是抗击冠状病毒爆发的多功能工具。

IF 2.7 4区生物学

Glycoconjugate Journal Pub Date : 2023-02-01 Epub Date: 2022-11-24 DOI: 10.1007/s10719-022-10094-4

Mathias Simplicien, Pierre Pério, Jan Sudor, Annick Barre, Hervé Benoist, Els J M Van Damme, Pierre Rougé

{"title":"Plant lectins as versatile tools to fight coronavirus outbreaks.","authors":"Mathias Simplicien, Pierre Pério, Jan Sudor, Annick Barre, Hervé Benoist, Els J M Van Damme, Pierre Rougé","doi":"10.1007/s10719-022-10094-4","DOIUrl":"10.1007/s10719-022-10094-4","url":null,"abstract":"The S protein forming the homotrimeric spikes of pathogenic beta-coronaviruses, such as MERS-CoV, SARS-CoV and SARS-CoV-2, is a highly glycosylated protein containing mainly N-glycans of the complex and high-mannose type, as well as O-glycans. Similarly, the host cell receptors DPP4 for MERS-CoV and ACE2 for SARS-CoV and SARS-CoV-2, also represent N- and O-glycosylated proteins. All these glycoproteins share common glycosylation patterns, suggesting that plant lectins with different carbohydrate-binding specificities could be used as carbohydrate-binding agents for the spikes and their receptors, to combat COVID19 pandemics. The binding of plant lectins to the spikes and their receptors could mask the non-glycosylated receptor binding domain of the virus and the corresponding region of the receptor, thus preventing a proper interaction of the spike proteins with their receptors. In this review, we analyze (1) the ability of plant lectins to interact with the N- and O-glycans present on the spike proteins and their receptors, (2) the in vitro and in vivo anti-COVID19 activity already reported for plant lectins and, (3) the possible ways for delivery of lectins to block the spikes and/or their receptors.","PeriodicalId":12762,"journal":{"name":"Glycoconjugate Journal","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9684959/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10731103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Afterword (Editorial). 后记（社论）。

IF 2.7 4区生物学

Glycoconjugate Journal Pub Date : 2023-02-01 Epub Date: 2022-11-02 DOI: 10.1007/s10719-022-10090-8

Kazuhisa Iwabuchi, Alessandro Prinetti

引用次数: 0

Partial characterization of purified glycoprotein from nutshell of Arachis hypogea L. towards macrophage activation and leishmaniacidal activity. 从榛子果壳中纯化的糖蛋白对巨噬细胞活化和利什曼杀虫活性的部分表征。

IF 2.7 4区生物学

Glycoconjugate Journal Pub Date : 2023-02-01 Epub Date: 2023-01-03 DOI: 10.1007/s10719-022-10096-2

Sujatha Srinivasan, Mamilla R Charan Raja, Amrita Kar, Aishwarya Ramasamy, Adithyan Jayaraman, Vellingiri Vadivel, Santanu Kar Mahapatra

{"title":"Partial characterization of purified glycoprotein from nutshell of Arachis hypogea L. towards macrophage activation and leishmaniacidal activity.","authors":"Sujatha Srinivasan, Mamilla R Charan Raja, Amrita Kar, Aishwarya Ramasamy, Adithyan Jayaraman, Vellingiri Vadivel, Santanu Kar Mahapatra","doi":"10.1007/s10719-022-10096-2","DOIUrl":"10.1007/s10719-022-10096-2","url":null,"abstract":"Arachis hypogea L. protein fraction-2 (AHP-F2) from the Peanut shell was extracted and characterized and its potent immunomodulatory and anti-leishmanial role was determined in this present study. AHP-F2 was found to be a glycoprotein as the presence of carbohydrates were confirmed by the analysis of high-performance liquid chromatography (HPLC) yielded glucose, galactose, mannose, and xylose. AHP-F2 molecular mass was found to be ∼28 kDa as indicated in MALDI-TOF and peptide mass fingerprinting analysis followed by Mascot search. The peptide matches revealed the similarity of the mannose/glucose binding lectin with 71.07% in the BLAST analysis. After that, the 3D structure of the AHP-F2 model was designed and validated by the Ramachandran plot. The immunomodulatory role of AHP-F2 was established in murine peritoneal macrophages as induction of nitric oxide (NO), and stimulation of proinflammatory cytokines (IL-12 and IFN-γ) in a dose-dependent manner was observed. Interestingly, it was also found that AHP-F2 has interacted with the innate immune receptor, toll-like receptors (TLRs) as established in molecular docking as well as mRNA expression. The anti-leishmanial potential of AHP-F2 was revealed with a prominent inhibition of amastigote growth within the murine macrophages with prompt induction of nitrite release. Altogether, the isolated AHP-F2 from Arachis hypogea L. has strong immunomodulatory and anti-leishmanial potential which may disclose a new path to treat leishmaniasis.","PeriodicalId":12762,"journal":{"name":"Glycoconjugate Journal","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10736164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A galactoside-specific Dalbergieae legume lectin from seeds of Vataireopsis araroba (Aguiar) Ducke. 一种来自 Vataireopsis araroba (Aguiar) Ducke 种子的半乳糖苷特异性豆科凝集素。

IF 2.7 4区生物学

Glycoconjugate Journal Pub Date : 2023-02-01 Epub Date: 2022-10-26 DOI: 10.1007/s10719-022-10082-8

Vinicius J S Osterne, Messias V Oliveira, Kristof De Schutter, Sonia Serna, Niels-Christian Reichardt, Guy Smagghe, Benildo S Cavada, Els J M Van Damme, Kyria Santiago Nascimento

{"title":"A galactoside-specific Dalbergieae legume lectin from seeds of Vataireopsis araroba (Aguiar) Ducke.","authors":"Vinicius J S Osterne, Messias V Oliveira, Kristof De Schutter, Sonia Serna, Niels-Christian Reichardt, Guy Smagghe, Benildo S Cavada, Els J M Van Damme, Kyria Santiago Nascimento","doi":"10.1007/s10719-022-10082-8","DOIUrl":"10.1007/s10719-022-10082-8","url":null,"abstract":"The Dalbergieae lectin group encompasses several lectins with significant differences in their carbohydrate specificities and biological properties. The current work reports on the purification and characterization of a GalNAc/Gal-specific lectin from Vataireopsis araroba (Aguiar) Ducke, designated as VaL. The lectin was purified from the seeds in a single step using guar gum affinity chromatography. The lectin migrated as a single band of about 35 kDa on SDS-PAGE and, in native conditions, occurs as a homodimer. The purified lectin is stable at temperatures up to 60 °C and in a pH range from 7 to 8 and requires divalent cations for its activity. Sugar-inhibition assays demonstrate the lectin specificity towards N-acetyl-D-galactosamine, D-galactose and related sugars. Furthermore, glycan array analyses show that VaL interacts preferentially with glycans containing terminal GalNAc/Galβ1-4GlcNAc. Biological activity assays were performed using three insect cell lines: CF1 midgut cells from the spruce budworm Choristoneura fumiferana, S2 embryo cells from the fruit fly Drosophila melanogaster, and GutAW midgut cells from the corn earworm Helicoverpa zea. In vitro assays indicated a biostatic effect for VaL on CF1 cells, but not on S2 and GutAW cells. The lectin presented a biostatic effect by reducing the cell growth and inducing cell agglutination, suggesting an interaction with glycans on the cell surface. VaL has been characterized as a galactoside-specific lectin of the Dalbergieae tribe, with sequence similarity to lectins from Vatairea and Arachis.","PeriodicalId":12762,"journal":{"name":"Glycoconjugate Journal","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10718466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0