Glycoconjugate Journal最新文献_第7页

ST3GalIV drives SLeX biosynthesis in gastrointestinal cancer cells and associates with cancer cell motility. ST3GalIV驱动胃肠道癌细胞的SLeX生物合成并与癌细胞运动相关。

IF 3 4区生物学

Glycoconjugate Journal Pub Date : 2023-08-01 DOI: 10.1007/s10719-023-10113-y

Ana F Costa, Emanuel Senra, Isabel Faria-Ramos, Andreia Teixeira, João Morais, Mariana Pacheco, Celso A Reis, Catarina Gomes

{"title":"ST3GalIV drives SLeX biosynthesis in gastrointestinal cancer cells and associates with cancer cell motility.","authors":"Ana F Costa, Emanuel Senra, Isabel Faria-Ramos, Andreia Teixeira, João Morais, Mariana Pacheco, Celso A Reis, Catarina Gomes","doi":"10.1007/s10719-023-10113-y","DOIUrl":"https://doi.org/10.1007/s10719-023-10113-y","url":null,"abstract":"Expression of sialyl Lewis X (SLeX) is a well-documented event during malignant transformation of cancer cells, and largely associates with their invasive and metastatic properties. Glycoproteins and glycolipids are the main carriers of SLeX, whose biosynthesis is known to be performed by different glycosyltransferases, namely by the family of β-galactoside-α2,3-sialyltransferases (ST3Gals). In this study, we sought to elucidate the role of ST3GalIV in the biosynthesis of SLeX and in malignant properties of gastrointestinal (GI) cancer cells. By immunofluorescent screening, we selected SLeX-positive GI cancer cell lines and silenced ST3GalIV expression via CRISPR/Cas9. Flow cytometry, immunofluorescence and western blot analysis showed that ST3GalIV KO efficiently impaired SLeX expression in most cancer cell lines, with the exception of the colon cancer cell line LS174T. The impact of ST3GalIV KO in the biosynthesis of SLeX isomer SLeA and non sialylated Lewis X and A were also evaluated and overall, ST3GalIV KO led to a decreased expression of SLeA and an increased expression in both LeX and LeA. In addition, the abrogation of SLeX on GI cancer cells led to a reduction in cell motility. Furthermore, ST3GalVI KO was performed in LS174T ST3GalIV KO cells, resulting in the complete abolishment of SLeX expression and consequent reduced motility capacity of those cells. Overall, these findings portray ST3GalIV as the main, but not the only, enzyme driving the biosynthesis of SLeX in GI cancer cells, with a functional impact on cancer cell motility.","PeriodicalId":12762,"journal":{"name":"Glycoconjugate Journal","volume":"40 4","pages":"421-433"},"PeriodicalIF":3.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10335957/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10584033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mass photometry: A powerful tool for carbohydrates-proteins conjugation monitoring and glycoconjugates molecular mass determination. 质谱法:用于碳水化合物-蛋白质偶联监测和糖缀合物分子质量测定的强大工具。

IF 3 4区生物学

Glycoconjugate Journal Pub Date : 2023-08-01 DOI: 10.1007/s10719-023-10126-7

Di Wu, Peng Xu, Meagan Kelly, Edward T Ryan, Pavol Kováč, Grzegorz Piszczek

{"title":"Mass photometry: A powerful tool for carbohydrates-proteins conjugation monitoring and glycoconjugates molecular mass determination.","authors":"Di Wu, Peng Xu, Meagan Kelly, Edward T Ryan, Pavol Kováč, Grzegorz Piszczek","doi":"10.1007/s10719-023-10126-7","DOIUrl":"https://doi.org/10.1007/s10719-023-10126-7","url":null,"abstract":"Glycoconjugate vaccines are important additions to the existing means for prevention of diseases caused by bacterial and viral pathogens. Conjugating carbohydrates to proteins is a crucial step in the development of these vaccines. Traditional mass spectrometry techniques, such as MALDI-TOF and SELDI-TOF, have difficulties in detecting glycoconjugates with high molecular masses. Mass photometry (MP) is a single-molecule technique that has been recently developed, which allows mass measurements of individual molecules and generates mass distributions based on hundreds to thousands of these measurements. In this study, we evaluated the performance of MP in monitoring carbohydrate-protein conjugation reactions and characterization of conjugates. Three different glycoconjugates were prepared from carrier protein BSA, and one from a large protein complex, a virus capsid with 3.74 MDa molecular mass. The masses measured by MP were consistent with those obtained by SELDI-TOF-MS and SEC-MALS. The conjugation of BSA dimer to carbohydrate antigen was also successfully characterized. This study shows that the MP technique is a promising alternative to methods developed earlier for monitoring glycoconjugation reactions and characterization of glycoconjugates. It measures intact molecules in solution and it is highly accurate over a wide mass range. MP requires only a very small amount of sample and has no specific buffer constraints. Other MP advantages include minimal cost of consumables and rapid data collection and analysis. Its advantages over other methods make it a valuable tool for researchers in the glycoconjugation field.","PeriodicalId":12762,"journal":{"name":"Glycoconjugate Journal","volume":"40 4","pages":"401-412"},"PeriodicalIF":3.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10374364/pdf/nihms-1914410.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10228308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The generation of 5-N-glycolylneuraminic acid as a consequence of high levels of reactive oxygen species. 由于活性氧含量高而产生的5- n -糖基神经氨酸。

IF 3 4区生物学

Glycoconjugate Journal Pub Date : 2023-08-01 DOI: 10.1007/s10719-023-10121-y

Ruifeng Bai, Jingyi Wang, Inka Brockhausen, Yin Gao

引用次数: 0

Recognition factors of Dolichos biflorus agglutinin (DBA) and their accommodation sites. 芍药凝集素(DBA)的识别因子及其调节位点。

IF 3 4区生物学

Glycoconjugate Journal Pub Date : 2023-08-01 DOI: 10.1007/s10719-023-10118-7

Albert M Wu, Anna Dudek, Yung Liang Chen

{"title":"Recognition factors of Dolichos biflorus agglutinin (DBA) and their accommodation sites.","authors":"Albert M Wu, Anna Dudek, Yung Liang Chen","doi":"10.1007/s10719-023-10118-7","DOIUrl":"https://doi.org/10.1007/s10719-023-10118-7","url":null,"abstract":"Dolichos biflorus agglutinin (DBA) is one of the well known plant lectins that are widely used in clinical serology to differentiate human blood group A1 and A2 erythrocytes and also applied to glycobiology. However, the knowledge of recognition factors of polyvalent (super) glycotopes in glycans and the roles of functional group and epimer in monosaccharide (sub-monosaccharide recognition factor) have not been well established. The size and shape of the recognition (combining) site of DBA has not been clearly defined. In this study, many importnat recognition factors of DBA-glycan binding were characterized by our established enzyme-linked lectinosorbent (ELLSA) and inhibition assays. The results of these assays showed that the intensity profile of the recognition factors for the major combining site of DBA was expressed by Mass relative potency (Mass R.P.) and shown by decreasing order of high density of polyvalent GalNAcα1 → (super glycotopes, 3.7 × 103) >> the corresponding β anomers >> monomeric GalNAcα1 → related glycotopes (GalNAc as 1.0) >> their GalNAc β-anomers >> Gal (absence of NHCH3CO at carbon-2 of GAlNAc) and GlcNAc (different epimer of Carbon-4 in GalNAc). From the all data available, it is proposed that the combining site of DBA should consist of a small cavity shape as major site and most complementary to monomeric GalNAcα → located at both terminal reducing end (Tn) and nonreducing end of glycan chains, and with a wide and broad area as subsite to accomodate from mono- to tetra-saccharides (GalNAcβ, Galβ1 → 3/4GlcNAc, lFuc1 → 2Galβ1 → 3/4GlcNAc, GalNAcβ1 → 3Galα1 → 4Galβ1 → 4Glc) at the nonreducing side. In this study, it has provided the most (comprehensive) recognition knowledge of DBA-glycan interactions at the factors of glycotope, super glycotope/sub-monosaccharide levels. Thus, it should expand and upgrade the conventional concept of the combining (recognition) site of DBA since 1980s.","PeriodicalId":12762,"journal":{"name":"Glycoconjugate Journal","volume":"40 4","pages":"383-399"},"PeriodicalIF":3.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10239814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Neuroimmunomodulatory properties of polysialic acid. 聚唾液酸的神经免疫调节特性。

IF 3 4区生物学

Glycoconjugate Journal Pub Date : 2023-06-01 DOI: 10.1007/s10719-023-10120-z

Lina Gretenkort, Hauke Thiesler, Herbert Hildebrandt

引用次数: 0

Vibrio-binding gangliosides in fish intestinal tracts. 鱼类肠道中与弧菌结合的神经节苷。

IF 3 4区生物学

Glycoconjugate Journal Pub Date : 2023-06-01 DOI: 10.1007/s10719-023-10110-1

Makoto Ito, Shinichi Chisada, Naoyuki Matsunaga, Nozomu Okino

引用次数: 0

Analysis of the intramolecular 1,7-lactone of N-acetylneuraminic acid using HPLC-MS: relationship between detection and stability. n -乙酰神经氨酸分子内1,7-内酯的HPLC-MS分析:检测与稳定性的关系。

IF 3 4区生物学

Glycoconjugate Journal Pub Date : 2023-06-01 DOI: 10.1007/s10719-023-10114-x

Paolo La Rocca, Ivana Lavota, Marco Piccoli, Federica Cirillo, Andrea Ghiroldi, Giuseppe Ciconte, Carlo Pappone, Pietro Allevi, Paola Rota, Luigi Anastasia

{"title":"Analysis of the intramolecular 1,7-lactone of N-acetylneuraminic acid using HPLC-MS: relationship between detection and stability.","authors":"Paolo La Rocca, Ivana Lavota, Marco Piccoli, Federica Cirillo, Andrea Ghiroldi, Giuseppe Ciconte, Carlo Pappone, Pietro Allevi, Paola Rota, Luigi Anastasia","doi":"10.1007/s10719-023-10114-x","DOIUrl":"https://doi.org/10.1007/s10719-023-10114-x","url":null,"abstract":"A subclass of the sialic acid family consists of intramolecular lactones that may function as key indicators of physiological and pathological states. However, the existence of these compounds in free form is highly improbable, since they are unlikely to exist in an aqueous solution due to their lability. Current analytical method used to detect them in biological fluids has not recognized their reactivity in solution and is prone to misidentification. However, recent advances in synthetic methods for 1,7-lactones have allowed the preparation of these sialic acid derivatives as authentic reference standards. We report here the development of a new HPLC-MS method for the simultaneous detection of the 1,7-lactone of N-acetylneuraminic acid, its γ-lactone derivative, and N-acetylneuraminic acid that overcomes the limitations of the previous analytical procedure for their identification.","PeriodicalId":12762,"journal":{"name":"Glycoconjugate Journal","volume":"40 3","pages":"343-354"},"PeriodicalIF":3.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10202997/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9885323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sialylation of cell surface glycoconjugates modulates cytosolic galectin-mediated responses upon organelle damage : Minireview. 细胞表面糖缀合物的唾液化调节细胞器损伤时细胞内半乳糖凝集素介导的反应。

IF 3 4区生物学

Glycoconjugate Journal Pub Date : 2023-06-01 DOI: 10.1007/s10719-023-10112-z

I-Chun Weng, Hung-Lin Chen, Wei-Han Lin, Fu-Tong Liu

引用次数: 0

Recently developed glycosphingolipid probes and their dynamic behavior in cell plasma membranes as revealed by single-molecule imaging. 近年来研制的鞘糖脂探针及其在细胞膜中的动态行为的单分子成像研究。

IF 3 4区生物学

Glycoconjugate Journal Pub Date : 2023-06-01 DOI: 10.1007/s10719-023-10116-9

Kenichi G N Suzuki, Naoko Komura, Hiromune Ando

{"title":"Recently developed glycosphingolipid probes and their dynamic behavior in cell plasma membranes as revealed by single-molecule imaging.","authors":"Kenichi G N Suzuki, Naoko Komura, Hiromune Ando","doi":"10.1007/s10719-023-10116-9","DOIUrl":"https://doi.org/10.1007/s10719-023-10116-9","url":null,"abstract":"Glycosphingolipids, including gangliosides, are representative lipid raft markers that perform a variety of physiological roles in cell membranes. However, studies aimed at revealing their dynamic behavior in living cells are rare, mostly due to a lack of suitable fluorescent probes. Recently, the ganglio-series, lacto-series, and globo-series glycosphingolipid probes, which mimic the behavior of the parental molecules in terms of partitioning to the raft fraction, were developed by conjugating hydrophilic dyes to the terminal glycans of glycosphingolipids using state-of-art entirely chemical-based synthetic techniques. High-speed, single-molecule observation of these fluorescent probes revealed that gangliosides were scarcely trapped in small domains (100 nm in diameter) for more than 5 ms in steady-state cells, suggesting that rafts including gangliosides were always moving and very small. Furthermore, dual-color, single-molecule observations clearly showed that homodimers and clusters of GPI-anchored proteins were stabilized by transiently recruiting sphingolipids, including gangliosides, to form homodimer rafts and the cluster rafts, respectively. In this review, we briefly summarize recent studies, the development of a variety of glycosphingolipid probes as well as the identification of the raft structures including gangliosides in living cells by single-molecule imaging.","PeriodicalId":12762,"journal":{"name":"Glycoconjugate Journal","volume":"40 3","pages":"305-314"},"PeriodicalIF":3.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9518978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synthesis and biological activity of ganglioside GM3 analogues with a (S)-CHF-Sialoside linkage and an alkyne tag. 具有(S)- chf -硅苷键和炔标记的神经节苷甘油酯GM3类似物的合成和生物活性。

IF 3 4区生物学

Glycoconjugate Journal Pub Date : 2023-06-01 DOI: 10.1007/s10719-023-10111-0

Eisuke Ota, Daiki Takeda, Kana Oonuma, Marie Kato, Hiroaki Matoba, Makoto Yoritate, Mikiko Sodeoka, Go Hirai

引用次数: 0