Glycoconjugate Journal最新文献

Exploring the impact of advanced glycation end products on diabetic salivary gland dysfunctions.

IF 2.7 4区生物学

Glycoconjugate Journal Pub Date : 2025-03-25 DOI: 10.1007/s10719-025-10182-1

Heba A Hassan

{"title":"Exploring the impact of advanced glycation end products on diabetic salivary gland dysfunctions.","authors":"Heba A Hassan","doi":"10.1007/s10719-025-10182-1","DOIUrl":"https://doi.org/10.1007/s10719-025-10182-1","url":null,"abstract":"The role of Advanced Glycation End Products (AGEs) in the pathophysiology of salivary gland dysfunction in diabetes has not been fully addressed. In this work, we discuss the pathophysiological mechanisms of salivary gland dysfunctions in diabetes, focusing on the role of AGEs. Hyperglycemia induces the generation and accumulation of AGEs, induces oxidative stress, and activates the receptor for AGEs (RAGE), with detrimental effects on the salivary glands and the submandibular autonomic innervation. Structural and ultrastructural alterations have been described in the three major salivary glands, and hypo-salivation development has been linked to early autonomic neuropathy. Poor metabolic control aggravates the salivary flow rate via injury to the autonomic nerve fiber bundles or direct damage to the secretory acinar cells of the glands. Chronic hyperglycemia, the most crucial feature of diabetes, leads to the generation and accumulation of advanced glycation end products (AGEs). The interest in the role of AGEs in the pathogenesis of diabetic complications has grown exponentially, and AGEs have been implicated as a primary culprit in the pathophysiology of diabetes and its various complications, including neuropathy, nephropathy, retinopathy, vasculopathy, and cardiomyopathy.","PeriodicalId":12762,"journal":{"name":"Glycoconjugate Journal","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143709557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Substrate flexibility of Mycoplasma fermentans mf1 phosphorylcholine transferase.

IF 2.7 4区生物学

Glycoconjugate Journal Pub Date : 2025-03-22 DOI: 10.1007/s10719-025-10181-2

Lena Nuschy, Biswajit Sarkar, Alla Zamyatina, Iain B H Wilson

{"title":"Substrate flexibility of Mycoplasma fermentans mf1 phosphorylcholine transferase.","authors":"Lena Nuschy, Biswajit Sarkar, Alla Zamyatina, Iain B H Wilson","doi":"10.1007/s10719-025-10181-2","DOIUrl":"https://doi.org/10.1007/s10719-025-10181-2","url":null,"abstract":"Zwitterionic modifications of glycans such as phosphorylcholine or phosphoethanolamine occur in a wide range of prokaryotic and eukaryotic organisms and are known for interaction with the mammalian immune system. Unlike the biosynthesis of membrane phospholipids which is well elucidated, very little is known about the transfer of zwitterionic phosphodiester moieties onto glycoconjugates. The presence and function of relevant enzymes has been suggested by gene knockout or mutation and corresponding aberrant phosphorylcholine metabolism. In the current study, the Mycoplasma fermentans phosphorylcholine transferase mf1, with previously confirmed in-vitro activity synthesizing phosphorylcholine-α-glucosyl-1,2-dipalmitoyl glycerol, is demonstrated to not only transfer phosphorylcholine but also phosphoethanolamine from CDP-ethanolamine. Moreover, mf1 is capable of using the β-configuration of the presumed natural substrate but transfers neither to simpler substrates with glucose moieties such as β-D-octyl-glucopyranoside nor to an extended lipid substrate with an additional galactose residue. These findings suggest a certain, but limited, substrate flexibility for bacterial PC-transferases. Mf1 activity is inhibited by β-glycerophosphate, an isomer of part of CDP-glycerol which is known to compete with CDP-ribitol in enzymatic reactions catalyzed by fukutin, a human protein sharing structural homology with mf1. For the first time, a phosphorylcholine transferase, mf1, could be biochemically characterized in vitro and its lipid products with zwitterionic phosphodiesters attached could be detected specifically with the pentraxin serum amyloid P.","PeriodicalId":12762,"journal":{"name":"Glycoconjugate Journal","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143691950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cartilaginous fishes-derived chondroitin sulfates potentially suppress lipid droplet accumulation in the differentiated 3T3-L1 adipocytes.

IF 2.7 4区生物学

Glycoconjugate Journal Pub Date : 2025-03-10 DOI: 10.1007/s10719-025-10183-0

Danang Dwi Cahyadi, Katsuhiko Warita, Naoko Takeda-Okuda, Jun-Ichi Tamura, Yoshinao Z Hosaka

{"title":"Cartilaginous fishes-derived chondroitin sulfates potentially suppress lipid droplet accumulation in the differentiated 3T3-L1 adipocytes.","authors":"Danang Dwi Cahyadi, Katsuhiko Warita, Naoko Takeda-Okuda, Jun-Ichi Tamura, Yoshinao Z Hosaka","doi":"10.1007/s10719-025-10183-0","DOIUrl":"https://doi.org/10.1007/s10719-025-10183-0","url":null,"abstract":"In this study, we investigated for cell proliferative and adipogenic differentiation inhibitory activities of chondroitin sulfate (CS) from cartilaginous fish: mako shark (Isurus oxyrinchus, spine part, Ms-CS), blue shark (Prionace glauca, spine part, Bs-CS), sharpspine skate (Okamejei acutispina, head and tail parts, Sp-CS) and stingray (Dasyatis akajei, head part, St-CS) on 3T3-L1 cells. Most of the CSs from cartilaginous fish showed concentration-dependent cell proliferative activity of 3T3-L1 cells within the retrieved concentration range (0-1,000 μg/mL), while under induction of adipocyte differentiation, they inhibited lipid accumulation. In particular, Ms-CS and Sp-CS were highly active in inhibiting lipid accumulation in the cells. The present study revealed that cartilaginous fish-derived CS has inhibitory activity on 3T3-L1 adipocyte differentiation by suppressing lipid droplet accumulation, although the degree of suppression varied depending on the composition of the CS and its origin. In addition, a significant increase in chondroitin sulfate N-acetylgalactosaminyltransferase 2 (Csgalnact2) expression of the Sp-CS group at the concentration of 500 µg/mL was observed. Csgalnact2 expression is associated with chondroitin N-acetylgalactosaminyltransferase-2 (ChGn-2), one of the glycosyltransferases that catalyzes the chain initiation and elongation of the CS backbone in its biosynthesis. Exogenous CS from cartilaginous fishes increased Csgalnact2 expression, although further studies are needed to confirm changes in CS biosynthesis. We observed reduced lipid accumulation in differentiated 3T3-L1 cells. Our findings highlight the role of CS polysaccharides, in inhibiting adipogenesis, even though further investigation is required to understand the underlying mechanism.","PeriodicalId":12762,"journal":{"name":"Glycoconjugate Journal","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143596748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Plant glycosides and glycosidases: classification, sources, and therapeutic insights in current medicine.

IF 2.7 4区生物学

Glycoconjugate Journal Pub Date : 2025-02-24 DOI: 10.1007/s10719-025-10180-3

Kumaresan Kowsalya, Nandakumar Vidya, Jayachandran Halka, Jaganathan Sakthi Yazhini Preetha, Muthukrishnan Saradhadevi, Jesudass Joseph Sahayarayan, Packiaraj Gurusaravanan, Muthukrishnan Arun

{"title":"Plant glycosides and glycosidases: classification, sources, and therapeutic insights in current medicine.","authors":"Kumaresan Kowsalya, Nandakumar Vidya, Jayachandran Halka, Jaganathan Sakthi Yazhini Preetha, Muthukrishnan Saradhadevi, Jesudass Joseph Sahayarayan, Packiaraj Gurusaravanan, Muthukrishnan Arun","doi":"10.1007/s10719-025-10180-3","DOIUrl":"https://doi.org/10.1007/s10719-025-10180-3","url":null,"abstract":"Plant glycosides have a broad spectrum of pharmaceutical activities primarily due to the glycosidic residues present in their structure. Especially, the therapeutic glycosides can be classified into many compounds based on the sugar moiety, chains/ saccharide units, glycosidic linkages, and aglycones. Among many classes, the widely used pharmacological classification is based on the aglycones linked to the glycoside molecule. Based on these non-sugar moiety (aglycones), plant glycosides are further classified into twelve different types of glycosides along with the recent discovery of novel (cannabinoid) glycosides. They are called alcoholic, anthraquinone, coumarin, chromone, cyanogenic, flavonoid, phenolic, cardiac, saponin, thio, steviol, iridoid, and cannabinoid glycosides. Each of the plant glycosides has been discussed in this paper with, origin, structure, and abundant presence in a specific family of plants. Besides, the therapeutic roles of these plant glycosides are further described in detail to validate their efficacies in the human health care system. On the other hand, glycosides are inactive until enzymatic hydrolysis releases their active aglycone, enabling targeted drug delivery. This process enhances aglycone solubility and stability, improving bioavailability and therapeutic efficacy. They target specific receptors or enzymes, minimizing off-target effects and enhancing pharmacological outcomes. Derived from plants, glycosides offer diverse chemical structures for drug development. They are integral to traditional medicine and modern pharmaceuticals, utilized in therapies ranging from cardiology to antimicrobial treatments.","PeriodicalId":12762,"journal":{"name":"Glycoconjugate Journal","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143482972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Computational insights into DC-SIGN's enhanced recognition of mannotriose CPS units via Ca²⁺ ion cross-talk.

IF 2.7 4区生物学

Glycoconjugate Journal Pub Date : 2025-02-19 DOI: 10.1007/s10719-025-10179-w

Hemchandra Deka, Arabinda Ghosh, Debabrat Baishya

{"title":"Computational insights into DC-SIGN's enhanced recognition of mannotriose CPS units via Ca2+ ion cross-talk.","authors":"Hemchandra Deka, Arabinda Ghosh, Debabrat Baishya","doi":"10.1007/s10719-025-10179-w","DOIUrl":"https://doi.org/10.1007/s10719-025-10179-w","url":null,"abstract":"The Carbohydrate Recognition Domain (CRD) of immune system's c-type lectin receptors (CLRs) preferentially interacts with the Capsular Polysaccharides (CPS) units. Implicit Ca2+ ions are crucial to CRD function. Increment of the ionic concentration explicitly affects the CPS recognition by CRD many-fold. DC-SIGN is one such CLR that acts for the differential recognition of the microbial CPS. The CPS mannotriose had the lowest binding energy (ΔG -4.7 kcal/mol) and the maximum affinity for DC-SIGN with implicit Ca2+ ion. In the present investigation the ligand affinity increases with the rise of Ca2+ concentration up to 1.5 M. Again, within the CRD the residues viz; Glutamate (347), Proline (348), and Asparagine (349) (EPN) were reported previously as essential for CPS unit coordination. Our analysis demonstrated that besides the EPN residues, CPS unit interacts with the neighboring Asparagine (350), Glutamate (354) and Asparagine (355) residues. Thus, these residues were replaced one at a time with Alanine (a charge neutral residue) to test their effect on the contact event. The CRD loses its affinity for recognition on the N350A, E354A, and D355A substitutions. Thus, this heterogeneity of CRD recognition towards Carbohydrate provides fresh information about the immune system's theragnostic function. This new understanding of Ca2+-induced recognition may help design new theragnostic applications that boost our immune defenses against pathogenic evasion.","PeriodicalId":12762,"journal":{"name":"Glycoconjugate Journal","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143448914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of CFTR modulators Elexacaftor/Tezacaftor/Ivacaftor on lipid metabolism in human bronchial epithelial cells. CFTR调节剂Elexacaftor/Tezacaftor/Ivacaftor对人支气管上皮细胞脂质代谢的影响。

IF 2.7 4区生物学

Glycoconjugate Journal Pub Date : 2025-02-01 Epub Date: 2025-01-11 DOI: 10.1007/s10719-024-10174-7

Dorina Dobi, Nicoletta Loberto, Laura Mauri, Rosaria Bassi, Elena Chiricozzi, Giulia Lunghi, Massimo Aureli

{"title":"Effect of CFTR modulators Elexacaftor/Tezacaftor/Ivacaftor on lipid metabolism in human bronchial epithelial cells.","authors":"Dorina Dobi, Nicoletta Loberto, Laura Mauri, Rosaria Bassi, Elena Chiricozzi, Giulia Lunghi, Massimo Aureli","doi":"10.1007/s10719-024-10174-7","DOIUrl":"10.1007/s10719-024-10174-7","url":null,"abstract":"Cystic Fibrosis (CF) is a life-threatening hereditary disease resulting from mutations in the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) gene that encodes a chloride channel essential for ion transport in epithelial cells. Mutations in CFTR, notably the prevalent F508del mutation, impair chloride transport, severely affecting the respiratory system and leading to recurrent infections. Recent therapeutic advancements include CFTR modulators such as ETI, a combination of two correctors (Elexacaftor and Tezacaftor) and a potentiator (Ivacaftor), that can improve CFTR function in patients with the F508del mutation. This study investigated ETI's impact on the maturation of the mutated CFTR, the expression levels of its scaffolding proteins, and lipid composition of cells using bronchial epithelial cell lines expressing both wild-type and F508del CFTR. Our findings revealed that ETI treatment enhances CFTR and its scaffolding proteins expression and aids in rescuing mature F508del CFTR, causing also significant alterations in the lipid profile including reduced levels of lactosylceramide and increased content of gangliosides GM1 and GD1a. These changes were linked to ETI's influence on enzymes involved in the sphingolipid metabolism, in particular GM3 synthase and sialidase. Through this work, we aim to deepen understanding CFTR interactions with lipids, and to elucidate the mechanisms of action of CFTR modulators. Our findings may support the development of potential therapeutic strategies contributing to the ongoing efforts to design effective correctors and potentiators for CF treatment.","PeriodicalId":12762,"journal":{"name":"Glycoconjugate Journal","volume":" ","pages":"1-14"},"PeriodicalIF":2.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142964495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inhibition of chondroitin sulphate-degrading enzyme Chondroitinase ABC by dextran sulphate. 硫酸葡聚糖对硫酸软骨素降解酶ABC的抑制作用。

IF 2.7 4区生物学

Glycoconjugate Journal Pub Date : 2025-02-01 Epub Date: 2025-01-16 DOI: 10.1007/s10719-024-10175-6

Sagar Dalal, Rachana Pathak, Edward X S Moh, Nicolle H Packer

{"title":"Inhibition of chondroitin sulphate-degrading enzyme Chondroitinase ABC by dextran sulphate.","authors":"Sagar Dalal, Rachana Pathak, Edward X S Moh, Nicolle H Packer","doi":"10.1007/s10719-024-10175-6","DOIUrl":"10.1007/s10719-024-10175-6","url":null,"abstract":"Chondroitin sulphate (CS) is a sulphated glycosaminoglycan (GAG) polysaccharide found on proteoglycans (CSPGs) in extracellular and pericellular matrices. Chondroitinase ABC (CSase ABC) derived from Proteus vulgaris is an enzyme that has gained attention for the capacity to cleave chondroitin sulphate (CS) glycosaminoglycans (GAG) from various proteoglycans such as Aggrecan, Neurocan, Decorin etc. The substrate specificity of CSase ABC is well-known for targeting various structural motifs of CS chains and has gained popularity in the field of neuro-regeneration by selective degradation of CS GAG chains. Within this context, our investigation into the biochemistry of CSase ABC led us to a previously unreported inhibition of CSase ABC activity by Dextran Sulphate (DexS). To understand the inhibitory effects of DexS, we compared its inhibition of CSase ABC to that of other polysaccharides such as Heparan Sulphate, Heparin, Colominic Acid, Fucoidan, and Dextran. This analysis identified key structural factors such as monosaccharide composition and linkage, sulphation degree and overall charge as influencing CSase ABC inhibition. Remarkably, DexS emerged as a unique inhibitor of CSase ABC, with distinctive inhibitory effects that correlate with its chain length. DexS has been used to reliably induce ulcerative colitis in mice, effectively mimicking inflammatory bowel diseases in humans, and has been previously shown to inhibit both RNA polymerase and reverse transcriptase. Our investigation emphasizes the interplay between the properties of DexS and CSase ABC, providing significant insights into the utilization of polysaccharide-based inhibitors for modulating enzyme activity.","PeriodicalId":12762,"journal":{"name":"Glycoconjugate Journal","volume":" ","pages":"53-59"},"PeriodicalIF":2.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11839815/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143004204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SUMOylated GLUT1 inhibited the glycometabolism disorder in chondroctyes during osteoarthritis. SUMOylated GLUT1抑制骨关节炎期间软骨细胞糖代谢紊乱。

IF 2.7 4区生物学

Glycoconjugate Journal Pub Date : 2025-02-01 Epub Date: 2025-01-11 DOI: 10.1007/s10719-024-10176-5

Liwei Xiong

{"title":"SUMOylated GLUT1 inhibited the glycometabolism disorder in chondroctyes during osteoarthritis.","authors":"Liwei Xiong","doi":"10.1007/s10719-024-10176-5","DOIUrl":"10.1007/s10719-024-10176-5","url":null,"abstract":"Reduction of glucose transporter 1 (GLUT1), even deletion, may results in cartilage fibrosis and osteoarthritis. This study aims to investigate the SUMOylation of GLUT1 in osteoarthritis through small ubiquitin-like modifier 1(SUMO1), and explore the role of SUMOylated GLUT1 in glycometabolism, proliferation and apoptosis in chondrocytes. Human chondrocytes were incubated with 10 ng/mL of IL-1β to mimic osteoarthritis in vitro. GLUT1, SUMO1 and Chondrocyte-related genes including COL2A1, MMP13 and ADAMTS4 were evaluated using western blot. Cell viability and cell apoptosis of chondrocytes were measured by cell counting kit-8 assay and flow cytometry, respectively. The changes in glycometabolism were evaluated using extracellular acidification rate (ECAR) and glucose uptake assay. Co-immunoprecipitation (Co-IP) was used to verify the interaction between GLUT1 and SUMO1. The stabilization role of SUMO1 in GLUT1 was determined by cycloheximide assay. IL-1β induced the decrease of GLUT1, cell viability, ECAR, glucose uptake and COL2A1 and the increase of cell apoptosis, MMP13 and ADAMTS4 in chondrocytes. However, overexpression of SUMO1 led to the reduction of cell apoptosis, MMP13 and ADAMTS4 and the elevation of GLUT1, cell viability, ECAR, glucose uptake and COL2A1 in IL-1β-stimulated chondrocytes. There was SUMOylation sites on GLUT1. Intriguingly, SUMO1 was significantly enriched in GLUT1 using Co-IP assay, and stabilized GLUT1 in chondrocytes. SUMO1-mediated SUMOylation is capable of stabilizing GLUT1 to inhibit glycometabilsm disorder and cell apoptosis in IL-1β-stimulated chondrocytes.","PeriodicalId":12762,"journal":{"name":"Glycoconjugate Journal","volume":" ","pages":"41-52"},"PeriodicalIF":2.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142964498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mechanistic studies of chondroitin sulfate/dermatan sulfate isolated from freshwater fish discards on osteogenesis in MC3T3-E1 cells.

IF 2.7 4区生物学

Glycoconjugate Journal Pub Date : 2025-02-01 Epub Date: 2025-01-30 DOI: 10.1007/s10719-025-10178-x

Chandra Gavva, Kunal Sharan, Nandini Chilkunda

{"title":"Mechanistic studies of chondroitin sulfate/dermatan sulfate isolated from freshwater fish discards on osteogenesis in MC3T3-E1 cells.","authors":"Chandra Gavva, Kunal Sharan, Nandini Chilkunda","doi":"10.1007/s10719-025-10178-x","DOIUrl":"10.1007/s10719-025-10178-x","url":null,"abstract":"Glycosaminoglycans (GAGs) are essential bone extracellular matrix molecules that regulate osteoblast differentiation. Numerous studies have explored endogenous and exogenous GAG osteoanabolic activities using appropriate in vitro and in vivo models. However, GAGs' underlying the mechanism of action and structure-function relationships need to be elucidated in detail. Earlier, we showed that exogenous GAG can bring about osteogenesis in pre-osteoblast cells. In the present study, we have elucidated the mechanism of action of exogenous GAGs, especially of the chondroitin sulfate/dermatan sulfate (CS/DS) class on osteogenesis. GAGs were immobilized, and osteoblast differentiation was evaluated in MC3T3-E1 cells. Results indicated that GAGs supported osteoblast differentiation by promoting collagen production, extracellular matrix formation, and subsequent mineralization. We elucidated the mechanisms underlying these effects by assessing the key signaling molecules involved in osteogenesis in response to exogenous CS/DS with/without BMP2. CS/DS alone significantly increased pERK1/2 and ATF4 expression levels differentially in a time-dependent manner without significant effects on BMP2, RUNX2, and pSMAD5 protein expression. On the other hand, CS/DS, in the presence of BMP2, differentially increased BMP2, pSMAD5, pERK1/2, RUNX2, and ATF4 expression levels at various time points. Collectively, these results strongly suggest that CS/DS can promote osteogenesis, and in the presence of BMP2, it could promote SMAD-mediated ERK-dependent osteogenesis.","PeriodicalId":12762,"journal":{"name":"Glycoconjugate Journal","volume":" ","pages":"15-26"},"PeriodicalIF":2.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143065100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spatial single-cell maps reveal ST6GAL1 promoting ovarian cancer metastasis.

IF 2.7 4区生物学

Glycoconjugate Journal Pub Date : 2025-02-01 Epub Date: 2025-01-30 DOI: 10.1007/s10719-025-10177-y

Lan-Hui Qin, Zijian Jiang, Chongze Yang, Rui Song, Pei-Yin Chen, Weihui Xu, Guanzhen Zeng, Jin-Yuan Liao, Liling Long

{"title":"Spatial single-cell maps reveal ST6GAL1 promoting ovarian cancer metastasis.","authors":"Lan-Hui Qin, Zijian Jiang, Chongze Yang, Rui Song, Pei-Yin Chen, Weihui Xu, Guanzhen Zeng, Jin-Yuan Liao, Liling Long","doi":"10.1007/s10719-025-10177-y","DOIUrl":"10.1007/s10719-025-10177-y","url":null,"abstract":"In this study, spatial and single-cell transcriptome techniques were used to investigate the role of beta-galactoside alpha-2,6-sialyltransferase 1 (ST6GAL1) in promoting peritoneal metastasis in ovarian cancer epithelial cells. We collected single-cell transcriptomic (GSE130000) and spatial transcriptomic datasets (GSE211956) from the Gene Expression Omnibus and RNA-sequencing data from The Cancer Genome Atlas. The Robust Cell Type Decomposition (RCTD) approach was implemented to integrate spatial and single-cell transcriptomic data. In addition, pseudo-time trajectory analysis, cell-cell communication networks, transcription factor activity profiling, spatial interaction mapping, and prognostic significance of gene expression were assessed. A significant enrichment of ST6GAL1 was observed in the epithelial cells of ovarian cancer, particularly in peritoneal metastases, which exhibited elevated metabolic activity compared to primary tumors. The levels of ST6GAL1 were significantly high in peritumoral and adjacent non-tumorous tissues, with increased metabolic activity, while the tumor core demonstrated ST6GAL1-negative epithelial cells. Extensive cell-cell communication and transcription factor networks were unraveled, potentially influencing vascular permeability and intracellular signaling. Clinically, high expression of ST6GAL1 in epithelial cells is associated with diminished progression-free survival, indicating its prognostic potential. In conclusion, ST6GAL1 is likely to significantly impact the progression and metastasis of ovarian cancer.","PeriodicalId":12762,"journal":{"name":"Glycoconjugate Journal","volume":" ","pages":"27-40"},"PeriodicalIF":2.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143065106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0