Glycoconjugate Journal最新文献

{"title":"Retraction Note: An unusual pro-inflammatory role of interleukin-10 induced by arabinosylated lipoarabinomannan in murine peritoneal macrophages.","authors":"Nivedita Majumder, Ranadhir Dey, Ram Kumar Mathur, Sriparna Datta, Madhumita Maitra, Sanjukta Ghosh, Bhaskar Saha, Subrata Majumdar","doi":"10.1007/s10719-026-10230-4","DOIUrl":"https://doi.org/10.1007/s10719-026-10230-4","url":null,"abstract":"","PeriodicalId":12762,"journal":{"name":"Glycoconjugate Journal","volume":"43 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149192935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of acylated/deacylated ghrelin, CCL20/CCR6 concentration, and IgG N-Glycan alterations in different dysplasia grades of colon adenoma.","authors":"Milena Hanžek, Andrea Tešija Kuna, Domagoj Kifer, Ivona Kološnjaj, Olga Gornik, Sanja Shapeski-Stojsavljević, Sandra Šupraha Goreta","doi":"10.1007/s10719-026-10229-x","DOIUrl":"10.1007/s10719-026-10229-x","url":null,"abstract":"<p><p>Colorectal adenomas represent precursor lesions in the adenoma-carcinoma sequence and are characterized by chronic low-grade inflammation. Chemokine CCL20/CCR6 signaling and metabolic hormone ghrelin have been implicated in colorectal tumorigenesis, while IgG glycosylation reflects systemic immune status. However, their interrelationship across different stages of adenoma dysplasia remains unclear. A total of 70 patients with histologically confirmed colorectal adenomas were stratified into low-grade (n = 36) and high-grade dysplasia (n = 34). Serum CCL20, CCR6, acylated and deacylated ghrelin concentrations were measured using enzyme immunoassay (ELISA), while IgG N-glycans were analyzed by hydrophilic interaction liquid chromatography-ultra performance liquid chromatography (HILIC-UPLC). Correlation analyses were performed to assess associations between inflammatory, metabolic, and glycomic parameters. No significant differences were observed between low- and high-grade dysplasia in circulating CCL20, CCR6, acylated or deacylated ghrelin, or IgG N-glycosylation profiles. A significant positive correlation between acylated and deacylated ghrelin was detected only in high-grade dysplasia. In low-grade dysplasia, CCL20 correlated significantly with deacylated ghrelin. Additional nominal associations between ghrelin, CCL20, and selected IgG glycan traits were identified but lost significance after Benjamini-Hochberg false discovery rate correction. These exploratory associations included bisecting glycans with deacylated ghrelin and CCL20 with GP20, GP21, GP1, and GP10. Although systemic inflammatory biomarkers, ghrelin isoforms, and IgG N-glycosylation did not differ between dysplasia grades, distinct correlation patterns suggest stage-specific relationships. The association between CCL20 and deacylated ghrelin in low-grade dysplasia and the strong correlation between ghrelin isoforms in high-grade dysplasia warrant further investigation.</p>","PeriodicalId":12762,"journal":{"name":"Glycoconjugate Journal","volume":"43 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149030502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of glycosylated small noncoding RNAs in tumor: mechanism, function and clinical significance.","authors":"Yanan Feng, Zhangren Chen, Yanqi Yang, Jiajun Cui, Qiaoli Lv, Shuhui Chen","doi":"10.1007/s10719-026-10228-y","DOIUrl":"10.1007/s10719-026-10228-y","url":null,"abstract":"<p><p>GlycoRNAs are a newly identified class of post‑transcriptionally modified small non‑coding RNAs that carry covalently attached N‑ and O‑linked glycans. This discovery challenges the conventional view that glycosylation is exclusive to proteins and lipids, and places RNA at the interface of glycobiology and immune recognition. Emerging evidence implicates glycoRNAs in tumor biology, where they function as cell‑surface ligands for Siglecs and P‑selectin, thereby modulating three interconnected processes: immune escape and immune regulation, intercellular communication and adhesion, and tumor metastasis and metabolic crosstalk. Recent technological advances-including single‑cell imaging (ARPLA), FRET‑based sEV analysis, and chemoenzymatic profiling-have enabled sensitive detection and revealed aberrant glycoRNA patterns across malignancies. Notably, tumor‑specific glycoRNA signatures correlate with immune checkpoint glycosylation (such as PD‑1/PD‑L1) and metabolic reprogramming. This review synthesizes current understanding of glycoRNA biosynthesis, structure, and function, with an emphasis on its emerging roles in the tumor microenvironment. We also outline the potential of RNA‑centric glycobiology for developing liquid biopsy biomarkers and mechanism‑informed immunotherapeutic strategies.</p>","PeriodicalId":12762,"journal":{"name":"Glycoconjugate Journal","volume":"43 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148982368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Structural dynamics and stability analysis of a novel ARSK variant confirms its role in MPS type X pathogenesis.","authors":"Muhammad Bilal, Muhammad Fakhar, Thamir Eid, Nawal Helmi, Misbahuddin Rafeeq, Mehreen Gul, Hammal Khan, Muhammad Umair","doi":"10.1007/s10719-026-10226-0","DOIUrl":"10.1007/s10719-026-10226-0","url":null,"abstract":"<p><p>Mucopolysaccharidoses (MPS) are heterogeneous group of disorders caused by the deficiency of lysosomal enzymatic activities. Deficiencies in these enzymes are required for proper degradation of glycosaminoglycans (GAGs), thus referred as inborn errors of lysosomal GAG metabolism. Arylsulfatase K (ARSK) has been recently characterized as lysosomal hydrolase involved in GAG degradation removing the 2-O-sulfate group from 2-sulfoglucuronate and has been associated with MPS X. A 12-year-old female presented with developmental delay, progressive musculoskeletal abnormalities, and skeletal dysplasia, with symptom onset at 2-3 years of age. Whole exome sequencing [WES], followed by bi-directional Sanger sequencing was performed to identify the culprit gene. As the three-dimensional structure of ARSK has not yet been experimentally resolved, an in silico predicted ARSK model was used for structural modeling, molecular dynamics simulations, and stability analyses to investigate the effect of the identified variant on protein structure and function. Biochemical and molecular analysis revealed likely pathogenic novel variant (c.238T > G; p.Cys80Gly) in the ARSK gene [NM_198150.3]. The identified sequence variant was screened in 2000 in-house exomes from the same ethnic population. Here, we report a novel variant in ARSK gene associated with MPS X, which will help in proper genetic counselling of the family.</p>","PeriodicalId":12762,"journal":{"name":"Glycoconjugate Journal","volume":"43 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148602183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Homo-oligomeric forms of lectins: their structural insights for glycan binding from the genus Canavalia.","authors":"Mahi Dev Ayikkara Peroor, Nivetha Ramanathan, Meenakumari Mani, Janarthanan Sundaram","doi":"10.1007/s10719-026-10222-4","DOIUrl":"10.1007/s10719-026-10222-4","url":null,"abstract":"<p><p>Lectins are made with identical polypeptide subunits as homo-oligomers in various species of Canavalia. Their interaction with the structures of specific glycans is influenced greatly when many identical polypeptides are cooperatively involved in a homo-oligomeric state than when they interact as individual polypeptide subunits. In the present study, lectins characterised from various species of Canavalia available in the literature were identified, and their sequences were retrieved to conduct in-silico bioinformatic analyses to offer a structural insight into their interaction with specific glycans such as D-mannose and. D-glucose. Our results disclosed that one of the critical parameters on the characteristic feature of lectins, i.e. the number of amino acids contributing to carbohydrate recognition domains (CRD) by lectin homo-oligomers in their tertiary and quaternary conformations through. in-silico docking, was less than in reported experimental studies conducted using XRD. Further, the docking results revealed that the kind of amino acids involved in recognising the structures of glycans was significantly different among homo-oligomeric polypeptide chains of various lectins, indicating the unique nature of each protein in their functions. The nature of glycan binding by these lectins was observed with higher affinity scores, with their quaternary structures containing four homo-oligomeric polypeptide chains. It is thus recognised that the unique nature of homo-oligomers of lectins from various species of Canavalia that possess analogous structural features relies profoundly on the kind of amino acid residues for their interaction with glycans in accomplishing their diverse functions.</p>","PeriodicalId":12762,"journal":{"name":"Glycoconjugate Journal","volume":"43 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148404481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to: Alpha-Gal syndrome unveiled: decoding the glycan-driven red meat allergy.","authors":"Łukasz F Sobala, Katarzyna Szymczak-Kulus, Łukasz Kasper, Marta Kasper, Radoslaw Kaczmarek, Marcin Czerwinski","doi":"10.1007/s10719-026-10224-2","DOIUrl":"10.1007/s10719-026-10224-2","url":null,"abstract":"","PeriodicalId":12762,"journal":{"name":"Glycoconjugate Journal","volume":"43 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148375268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Macrophage modulation accounts for the anti-inflammatory effect of Hypnea cervicornis agglutinin in rat arthritis induced by zymosan.","authors":"Francisco Glerison da Silva Nascimento, Pedro Henrique de Souza Ferreira Bringel, Diego Freitas de Araujo, Mário Rogério Lima Mota, Maria Gleiciane de Queiroz Martins, Mayara Torquato Lima da Silva, Clareane Avelino Simplício Nobre, Benildo Sousa Cavada, Kyria Santiago do Nascimento, Rondinelle Ribeiro Castro, Ana Maria Sampaio Assreuy","doi":"10.1007/s10719-026-10221-5","DOIUrl":"10.1007/s10719-026-10221-5","url":null,"abstract":"<p><p>Previous study demonstrated that Hypnea cervicornis lectin (HCA) reduces inflammation and nociception via interaction with the lectin carbohydrate-binding site, modulating gene expression of the interleukins IL-1β, TNF-α and of iNOS. This study evaluated the effect of HCA in the rat model of arthritis induced by zymosan in the tibiotarsal joint and the involvement of macrophage-derived mediators. In vitro, macrophages were stimulated with zymosan before being incubated with HCA and the supernatant was injected into the joint. In vivo, HCA was administered by intravenous route after intra-articular injection of zymosan, fMLP, or macrophages supernatant. Hypernociception, edema and leukocyte influx were evaluated in the joints, and inflammatory mediators in the macrophage supernatant or periarticular tissue. In vitro, HCA (100 µg/ml) reduced NO₂^- and IL-1β in the supernatant of macrophages. In vivo, HCA (3 mg/kg) reduced articular hypernociception, edema and leukocyte influx elicited by zymosan, fMLP or by the supernatant of zymosan activated macrophages, as well as the zymosan-induced rolling and adhesion, and gene expression of iNOS and IL-1. HCA attenuated edema and leukocyte influx in the periarticular tissue, revealing preserved chondrocytes in the cartilage. In conclusion, HCA exerts anti-inflammatory effect in the arthritis induced by zymosan in rat tibio-tarsal joints, involving NO and IL-1 released by resident macrophages.</p>","PeriodicalId":12762,"journal":{"name":"Glycoconjugate Journal","volume":"43 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13287125/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148294800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanistic and functional integration of sirtuin deacylases: from substrate specificity to biological outcomes.","authors":"Jian-Long Zhang, Zi-Yu Dou, Yong-Yue Fan, De-Guan Sun, Xiong-Jie Fan, Ze Yu, Guo-Cai Yang","doi":"10.1007/s10719-026-10223-3","DOIUrl":"10.1007/s10719-026-10223-3","url":null,"abstract":"<p><p>Sirtuins, as classic NAD⁺-dependent class III histone deacetylases (HDACs), have been the focus of extensive research since their discovery, primarily centered on the mechanisms by which their deacetylase activity mediates the pathogenesis of various diseases and regulates key biological processes. With the rapid advancement of epigenetics, numerous novel acylation modifications, including lysine malonylation (Kma), lysine β-hydroxybutyrylation (Kbhb), lysine succinylation (Ksucc), and have been identified to date. Notably, the recently discovered lysine lactylation (Kla) modification has fundamentally revised long-held perception of lactic acid as a mere \"metabolic waste\". Importantly, SIRT1-3 have been shown to possess robust delactylase activity. To date, SIRT1-7 have been discovered to exert over ten distinct novel enzymatic functions. Herein, we summarized the functions of SIRT1-7 and their associated metabolic regulatory pathways in the context of post-translational modifications.</p>","PeriodicalId":12762,"journal":{"name":"Glycoconjugate Journal","volume":"43 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148294852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nucleolar-localized sialidase Neu4 suppresses cell proliferation through destabilizing c-Myc via interacting with ribosomal proteins L5 and L11.","authors":"Toshiki Hyodo, Satsuki Yamate, Xiaoying Chen, Masaharu Komatsu, Kazuhiro Shiozaki","doi":"10.1007/s10719-026-10220-6","DOIUrl":"10.1007/s10719-026-10220-6","url":null,"abstract":"<p><p>Sialidase Neu4 is a glycosidase that removes sialic acids from nonreducing ends of sugar chains. Recently, Mexican tetra Neu4 (AmNeu4) was identified as a nuclear-localized sialidase. However, the subnuclear localization and biological functions of AmNeu4 have remained unclear. This study demonstrated that AmNeu4 localized in the nucleolus and investigated the significance of its nuclear localization signal (NLS) for the nucleolar localization. Green fluorescent protein (GFP) fused to NLS localized to the nucleolus. Additionally, deletion of the NLS in AmNeu4 abolished its nucleolar localization, and insertion of additional lysine/arginine residues into AmNeu4's NLS induced exclusive nucleolar localization. These results indicated that the nucleolar localization of AmNeu4 was mediated by its NLS. The proliferation of AmNeu4-overexpressing HeLa cells was significantly reduced compared with that of the mock-transfected cells. Moreover, enzymatically inactive AmNeu4 also suppressed cell growth, indicating that the enzymatic activity of AmNeu4 is not required for the suppression of cell growth. We hypothesized that the suppressed cell proliferation is mediated by protein-protein interactions involving AmNeu4. By immunoprecipitation assay, ribosomal proteins L5 and L11 (RPL5 and RPL11, respectively) were identified as AmNeu4 interacting partners. C-MYC protein levels were lower in AmNeu4-overexpressing cells than in mock cells, and these reduced levels were restored by RPL11 knockdown. Coimmunoprecipitation assay further revealed that RPL11 bound to C-MYC in AmNeu4-overexpressing cells. These results suggest that the interaction between AmNeu4 and RPL5/RPL11 delays cell growth through destabilizing C-MYC.</p>","PeriodicalId":12762,"journal":{"name":"Glycoconjugate Journal","volume":"43 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148054177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"3'-sialyllactose inhibits the migration and invasion of LPS-induced B16F10 mouse melanoma cells through inactivation of the NF-_KB signaling pathway.","authors":"Hee-Do Kim, Hyunju-Choi, Yu-Chan Chang, Cheorl-Ho Kim","doi":"10.1007/s10719-026-10219-z","DOIUrl":"10.1007/s10719-026-10219-z","url":null,"abstract":"<p><p>Malignant melanoma has a high mortality rate and is aggressive. The development, metastasis, and angiogenesis of melanoma have all been connected to toll-like receptor 4 (TLR4). However, signal transduction mediated by TLR4 for accelerating melanoma progression is fully unclear. Because of this, the current research has been carried out to explore drug candidate possibility using 3'-Sialyllactose (3'-SL). We investigated the inhibitory effect of 3'-SL on migration and invasion, which are associated with treatment difficulty and mortality. The suppression of matrix metalloproteinases 9 (MMP-9) expression and activity by 3'-SL in B16F10 murine melanoma cells and concurrently downregulated the MAPK signaling pathway involved in this regulation. Therefore, our results demonstrate that 3'-SL may be a good candidate for the development of therapeutic agents to suppress malignancy associated with metastasis and invasion.</p>","PeriodicalId":12762,"journal":{"name":"Glycoconjugate Journal","volume":"43 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13201296/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148015157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}