Glycoconjugate Journal最新文献_第10页

Glycoproteomic analysis reveals the effects of bisecting GlcNAc in intrahepatic cholangiocarcinoma. 糖蛋白组学分析揭示了分割GlcNAc在肝内胆管癌中的作用。

IF 3 4区生物学

Glycoconjugate Journal Pub Date : 2022-12-01 DOI: 10.1007/s10719-022-10085-5

Wei Dan, Cheng Li, Jun Li, Pengfei Li, Miaomiao Xin, Zexuan Chen, Liuyi Dang, Zihao Yu, Jing Li, Jiechen Shen, Liangshuo Hu, Shisheng Sun

{"title":"Glycoproteomic analysis reveals the effects of bisecting GlcNAc in intrahepatic cholangiocarcinoma.","authors":"Wei Dan, Cheng Li, Jun Li, Pengfei Li, Miaomiao Xin, Zexuan Chen, Liuyi Dang, Zihao Yu, Jing Li, Jiechen Shen, Liangshuo Hu, Shisheng Sun","doi":"10.1007/s10719-022-10085-5","DOIUrl":"https://doi.org/10.1007/s10719-022-10085-5","url":null,"abstract":"Intrahepatic cholangiocarcinoma (ICC) is the second major subtype of primary liver cancer and has caused more and more attention with increasing incidence and mortality worldwide. Our previous study found that bisecting N-glycans are commonly increased in ICC, while the effects and potential functions of bisecting GlcNAc in ICC are still largely unclear. In this study, we further confirmed that the structures of bisecting GlcNAc were significantly up-regulated in ICC compared with paracancer tissues by glycoproteomic data and lectin histochemistry. The expression of its glycosyltransferase MGAT3 was also up-regulated in ICC tissues at both mRNA and protein levels, and expression of MGAT3 is negatively correlated with overall survival explored by bioinformatic analyses and published datasets from 255 patients. Next, the silencing of MGAT3 could inhibit the growth and invasion of ICC cells, and overexpressing of MGAT3 only promoted ICC cell invasion. Further glycoproteomic analysis showed that the commonly glycoproteins modified by bisecting GlcNAc after MGAT3-overexpression in two ICC cell lines were mainly involved in cell movement-related biological processes, such as cell adhesion, integrin-related and ECM-receptor interaction. This study sheds light on the potential effects of bisecting GlcNAc in ICC cells and suggests that MGAT3 might be used as a potential target in the therapy of ICC.","PeriodicalId":12762,"journal":{"name":"Glycoconjugate Journal","volume":"39 6","pages":"737-745"},"PeriodicalIF":3.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10349626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of fermentation pH on the structure, rheological properties, and antioxidant activities of exopolysaccharides produced by Alteromonas australica QD. 发酵pH对澳大利亚异单胞菌胞外多糖结构、流变学特性及抗氧化活性的影响

IF 3 4区生物学

Glycoconjugate Journal Pub Date : 2022-12-01 DOI: 10.1007/s10719-022-10087-3

Fengshu Li, Xin Hu, Xiaojun Sun, Haoshuai Li, Jinren Lu, Yiming Li, Mutai Bao

{"title":"Effect of fermentation pH on the structure, rheological properties, and antioxidant activities of exopolysaccharides produced by Alteromonas australica QD.","authors":"Fengshu Li, Xin Hu, Xiaojun Sun, Haoshuai Li, Jinren Lu, Yiming Li, Mutai Bao","doi":"10.1007/s10719-022-10087-3","DOIUrl":"https://doi.org/10.1007/s10719-022-10087-3","url":null,"abstract":"The pH value was essential for the growth and metabolism of microorganisms. Acidic pH exopolysaccharide (AC-EPS) and alkaline pH exopolysaccharide (AL-EPS) secreted by A. australica QD mediated by pH were studied in this paper. The total carbohydrate content and molecular weight of AC-EPS (79.59% ± 2.24% (w/w), 8.374 × 105 Da) and AL-EPS (82.48% ± 1.46% (w/w), 6.182 × 105 Da) were estimated and compared. In AC-EPS, mannose (3.78%) and galactose (3.24%) content was more, while the proportion of glucuronic acid was less in comparison to AL-EPS. The scanning electron microscopy revealed the structural differences among the AC-EPS and AL-EPS. Thermogravimetric analysis showed degradation temperatures of 272.8 °C and 244.9 °C for AC-EPS and AL-EPS, respectively. AC-EPS was found to exhibit better rheological properties and emulsifying capabilities, while AL-EPS had superior antioxidant activities. Overall, both AC-EPS and AL-EPS have the potential to be used as emulsifiers and biological antioxidants.","PeriodicalId":12762,"journal":{"name":"Glycoconjugate Journal","volume":"39 6","pages":"773-787"},"PeriodicalIF":3.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10719413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

A water-soluble polysaccharide from Eucommia folium: the structural characterization and anti-tumor activity in vivo. 杜仲叶水溶性多糖的结构表征及体内抗肿瘤活性。

IF 3 4区生物学

Glycoconjugate Journal Pub Date : 2022-12-01 DOI: 10.1007/s10719-022-10086-4

Zhi-Qian Yan, Su-Yun Ding, Pei Chen, Hui-Ping Liu, Meng-Li Chang, Shu-Yuan Shi

{"title":"A water-soluble polysaccharide from Eucommia folium: the structural characterization and anti-tumor activity in vivo.","authors":"Zhi-Qian Yan, Su-Yun Ding, Pei Chen, Hui-Ping Liu, Meng-Li Chang, Shu-Yuan Shi","doi":"10.1007/s10719-022-10086-4","DOIUrl":"https://doi.org/10.1007/s10719-022-10086-4","url":null,"abstract":"In this study, a water-soluble polysaccharide from Eucommia folium was extracted by hot water and purified using Sephadex G-200 gel columns. The results showed that the purified fraction (EFP) has a molecular weight of 9.98 × 105 Da and consisted of rhamnose, arabinose, galactose, glucose, mannose, xylose, galacturonic acid, and glucuronic acid (molar ratio: 0.226: 1.739: 2.183: 1: 0.155: 0.321: 0.358: 0.047). The combination of infrared spectroscopy and NMR analysis proved that EFP is an acidic polysaccharide whose main chain consists of α-L-Araf-(1 → , → 3,5)-α-Araf-(1 → , → 3)-β-Galp-(1 → , → 3,6)-β-Glcp-(1 → , → 2)-α-D-Manp-(1 → , → 4)-α-GalpA-(1 → , → 2,4)-α-Rhap-(1 → . In addition, the in vivo antitumoral activity of EFP was studied using a H22 tumor-bearing mice model. EFP effectively inhibited tumor growth in mice following intragastric administration. By Combining with the results of the apoptosis assay and JC-1 staining analysis, we confirmed that EFP induces apoptosis through the mitochondrial pathway. Furthermore, cell cycle analysis demonstrated that EFP blocks the cell cycle at S phase.","PeriodicalId":12762,"journal":{"name":"Glycoconjugate Journal","volume":"39 6","pages":"759-772"},"PeriodicalIF":3.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10351406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Simple separation of glycosphingolipids in the lower phase of a Folch's partition from crude lipid fractions using zirconium dioxide. 使用二氧化锆从粗脂馏分中简单分离Folch分割下部相的鞘糖脂。

IF 3 4区生物学

Glycoconjugate Journal Pub Date : 2022-12-01 DOI: 10.1007/s10719-022-10080-w

Hideharu Nagasawa, Shota Miyazaki, Mamoru Kyogashima

{"title":"Simple separation of glycosphingolipids in the lower phase of a Folch's partition from crude lipid fractions using zirconium dioxide.","authors":"Hideharu Nagasawa, Shota Miyazaki, Mamoru Kyogashima","doi":"10.1007/s10719-022-10080-w","DOIUrl":"https://doi.org/10.1007/s10719-022-10080-w","url":null,"abstract":"A simple method was developed for the separation of glycosphingolipids (GSLs) from lipid mixtures, including phospholipids and cholesterol, using zirconium dioxide (zirconia, ZrO2). Although this procedure does not incorporate a mild alkali treatment, which is commonly used for eliminating glycerophospholipids, it can be used to remove both alkali-resistant sphingomyelin and glycerophospholipids possessing ether bonds. Importantly, when GSLs were dissolved in organic solvent together with cholesterol (Chol) and phospholipids, and loaded onto ZrO2, Chol did not bind to the ZrO2 but both the GSLs and phospholipids did. When eluted with 5 mg/mL of 2,5-dihydroxybenzoic acid in methanol, GSLs but not phospholipids were recovered, leaving the phospholipids bound to the ZrO2 particles. This method is particularly applicable for GSLs such as triglycosylceramides, tetraglycosylceramides and some pentaglycosylceramides, sulfatide and GM3 located in the lower phase of a Folch's partition, where significant amounts of phospholipids, Chol and neutral lipids reside along with GSLs. This method was successfully used to easily isolate GSLs from biological materials for their subsequent analysis by matrix-assisted laser desorption ionization time-of-flight mass spectrometry with high resolution.","PeriodicalId":12762,"journal":{"name":"Glycoconjugate Journal","volume":"39 6","pages":"789-795"},"PeriodicalIF":3.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10698951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

A homogalacturonan from Lonicera japonica Thunb. disrupts angiogenesis via epidermal growth factor receptor and Delta-like 4 associated signaling. 取自金银花(Lonicera japonica Thunb)的一种同型半乳糖酸。通过表皮生长因子受体和δ样4相关信号干扰血管生成。

IF 3 4区生物学

Glycoconjugate Journal Pub Date : 2022-12-01 DOI: 10.1007/s10719-022-10088-2

Wenfeng Liao, Xiaodong Hu, Zhenyun Du, Peipei Wang, Kan Ding

引用次数: 2

Thrombospondin type 1 repeat-derived C-mannosylated peptide attenuates synaptogenesis of cortical neurons induced by primary astrocytes via TGF-β. 血小板反应蛋白1型重复源性c -甘露糖基化肽通过TGF-β减弱原代星形胶质细胞诱导的皮质神经元突触发生。

IF 3 4区生物学

Glycoconjugate Journal Pub Date : 2022-10-01 Epub Date: 2021-11-18 DOI: 10.1007/s10719-021-10030-y

Kazuchika Nishitsuji, Midori Ikezaki, Shino Manabe, Kenji Uchimura, Yukishige Ito, Yoshito Ihara

{"title":"Thrombospondin type 1 repeat-derived C-mannosylated peptide attenuates synaptogenesis of cortical neurons induced by primary astrocytes via TGF-β.","authors":"Kazuchika Nishitsuji, Midori Ikezaki, Shino Manabe, Kenji Uchimura, Yukishige Ito, Yoshito Ihara","doi":"10.1007/s10719-021-10030-y","DOIUrl":"https://doi.org/10.1007/s10719-021-10030-y","url":null,"abstract":"C-Mannosylation is a rare type of protein glycosylation and is reportedly critical for the proper folding and secretion of parental proteins. Still, the effects of C-mannosylation on the biological functions of these modified proteins remain to be elucidated. The Trp-x-x-Trp (WxxW) sequences, whose first tryptophan (Trp) can be C-mannosylated, constitute the consensus motifs for this glycosylation modification and are commonly found in thrombospondin type 1 repeats that regulate molecular functions of thrombospondin 1 in binding and activation of transforming growth factor β (TGF-β). TGF-β plays critical roles in the control of the central nervous system including synaptogenesis. Here, we investigated whether C-mannosylation of the synthetic Trp-Ser-Pro-Trp (WSPW) peptide may confer certain functions to this peptide in TGF-β-mediated synaptogenesis. By using primary cultured rat astrocytes and cortical neurons, we found that the C-mannosylated WSPW (C-Man-WSPW) peptide, but not non-mannosylated WSPW peptide, suppressed astrocyte-conditioned medium (ACM)-stimulated synaptogenesis. C-Man-WSPW peptide inhibited both ACM- and recombinant mature TGF-β1-induced activations of Smad 2, an important mediator in TGF-β signaling. Interactions of recombinant mature TGF-β with the C-Man-WSPW peptide were similar to those with non-C-mannosylated WSPW peptide. Taken together, our results reveal a novel function of C-mannosylation of the WxxW motif in signaling and synaptogenesis mediated by TGF-β. Molecular details of how C-mannosylation affects the biological functions of WxxW motifs deserve future study for clarification.","PeriodicalId":12762,"journal":{"name":"Glycoconjugate Journal","volume":"39 5","pages":"701-710"},"PeriodicalIF":3.0,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39634371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Roland Schauer – Obituary Roland Schauer–讣告

IF 3 4区生物学

Glycoconjugate Journal Pub Date : 2022-08-03 DOI: 10.1007/s10719-022-10070-y

Anthony Corfield, J. Kamerling

引用次数: 0

Correction to: Role of sialylated glycans on bovine lactoferrin against influenza virus 更正：唾液酸化聚糖对牛乳铁蛋白对抗流感病毒的作用

IF 3 4区生物学

Glycoconjugate Journal Pub Date : 2022-06-10 DOI: 10.1007/s10719-022-10071-x

Xilong Wang, Lixin Yue, Liuyi Dang, Jiajun Yang, Zhuo Chen, Xiurong Wang, Jian Shu, Zheng Li

引用次数: 0

Sialidase NEU3 and its pathological significance 唾液酸酯酶NEU3及其病理意义