Glycoconjugate Journal最新文献_第10页

Thrombospondin type 1 repeat-derived C-mannosylated peptide attenuates synaptogenesis of cortical neurons induced by primary astrocytes via TGF-β. 血小板反应蛋白1型重复源性c -甘露糖基化肽通过TGF-β减弱原代星形胶质细胞诱导的皮质神经元突触发生。

IF 3 4区生物学

Glycoconjugate Journal Pub Date : 2022-10-01 Epub Date: 2021-11-18 DOI: 10.1007/s10719-021-10030-y

Kazuchika Nishitsuji, Midori Ikezaki, Shino Manabe, Kenji Uchimura, Yukishige Ito, Yoshito Ihara

{"title":"Thrombospondin type 1 repeat-derived C-mannosylated peptide attenuates synaptogenesis of cortical neurons induced by primary astrocytes via TGF-β.","authors":"Kazuchika Nishitsuji, Midori Ikezaki, Shino Manabe, Kenji Uchimura, Yukishige Ito, Yoshito Ihara","doi":"10.1007/s10719-021-10030-y","DOIUrl":"https://doi.org/10.1007/s10719-021-10030-y","url":null,"abstract":"C-Mannosylation is a rare type of protein glycosylation and is reportedly critical for the proper folding and secretion of parental proteins. Still, the effects of C-mannosylation on the biological functions of these modified proteins remain to be elucidated. The Trp-x-x-Trp (WxxW) sequences, whose first tryptophan (Trp) can be C-mannosylated, constitute the consensus motifs for this glycosylation modification and are commonly found in thrombospondin type 1 repeats that regulate molecular functions of thrombospondin 1 in binding and activation of transforming growth factor β (TGF-β). TGF-β plays critical roles in the control of the central nervous system including synaptogenesis. Here, we investigated whether C-mannosylation of the synthetic Trp-Ser-Pro-Trp (WSPW) peptide may confer certain functions to this peptide in TGF-β-mediated synaptogenesis. By using primary cultured rat astrocytes and cortical neurons, we found that the C-mannosylated WSPW (C-Man-WSPW) peptide, but not non-mannosylated WSPW peptide, suppressed astrocyte-conditioned medium (ACM)-stimulated synaptogenesis. C-Man-WSPW peptide inhibited both ACM- and recombinant mature TGF-β1-induced activations of Smad 2, an important mediator in TGF-β signaling. Interactions of recombinant mature TGF-β with the C-Man-WSPW peptide were similar to those with non-C-mannosylated WSPW peptide. Taken together, our results reveal a novel function of C-mannosylation of the WxxW motif in signaling and synaptogenesis mediated by TGF-β. Molecular details of how C-mannosylation affects the biological functions of WxxW motifs deserve future study for clarification.","PeriodicalId":12762,"journal":{"name":"Glycoconjugate Journal","volume":"39 5","pages":"701-710"},"PeriodicalIF":3.0,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39634371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Roland Schauer – Obituary Roland Schauer–讣告

IF 3 4区生物学

Glycoconjugate Journal Pub Date : 2022-08-03 DOI: 10.1007/s10719-022-10070-y

Anthony Corfield, J. Kamerling

引用次数: 0

Correction to: Role of sialylated glycans on bovine lactoferrin against influenza virus 更正：唾液酸化聚糖对牛乳铁蛋白对抗流感病毒的作用

IF 3 4区生物学

Glycoconjugate Journal Pub Date : 2022-06-10 DOI: 10.1007/s10719-022-10071-x

Xilong Wang, Lixin Yue, Liuyi Dang, Jiajun Yang, Zhuo Chen, Xiurong Wang, Jian Shu, Zheng Li

引用次数: 0

Sialidase NEU3 and its pathological significance 唾液酸酯酶NEU3及其病理意义

IF 3 4区生物学

Glycoconjugate Journal Pub Date : 2022-06-08 DOI: 10.1007/s10719-022-10067-7

T. Miyagi, Koji Yamamoto

引用次数: 8

Structural analysis and in vitro antitumor effect of polysaccharides from Pholiota adiposa 脂肪木多糖的结构分析及体外抗肿瘤作用

IF 3 4区生物学

Glycoconjugate Journal Pub Date : 2022-06-08 DOI: 10.1007/s10719-022-10065-9

Jiao Zhou, Jinhua Gong, Yangyang Chai, D. Li, Cong Zhou, Chuansheng Sun, J. Regenstein

引用次数: 2

Neuroinflammation and galectins: a key relationship in neurodegenerative diseases 神经炎症和凝集素:神经退行性疾病的关键关系

IF 3 4区生物学

Glycoconjugate Journal Pub Date : 2022-06-02 DOI: 10.1007/s10719-022-10064-w

Eleazar Ramírez Hernández, Beatriz Alanis Olvera, Daniela Carmona González, Oscar Guerrero Marín, Denisse Pantoja Mercado, Lucero Valencia Gil, L. Hernández-Zimbrón, José Luis Sánchez Salgado, I. D. Limón, E. Zenteno

引用次数: 5

Optimisation of enzyme-assisted extraction of Erythronium sibiricum bulb polysaccharide and its effects on immunomodulation. 丹参球茎多糖酶法提取工艺优化及其免疫调节作用。

IF 3 4区生物学

Glycoconjugate Journal Pub Date : 2022-06-01 Epub Date: 2022-02-09 DOI: 10.1007/s10719-021-10038-4

Shanshan Gao, Shujing Yan, Yue Zhou, Yue Feng, Xiangyun Xie, Wei Guo, Qi Shen, Chunli Chen

{"title":"Optimisation of enzyme-assisted extraction of Erythronium sibiricum bulb polysaccharide and its effects on immunomodulation.","authors":"Shanshan Gao, Shujing Yan, Yue Zhou, Yue Feng, Xiangyun Xie, Wei Guo, Qi Shen, Chunli Chen","doi":"10.1007/s10719-021-10038-4","DOIUrl":"https://doi.org/10.1007/s10719-021-10038-4","url":null,"abstract":"In this study, polysaccharides of Erythronium sibiricum bulb were extracted using enzyme-assisted extraction technology and then optimised by response surface methodology. The characteristics and immunomodulatory activities of the polysaccharide (E1P) were investigated. Setting the yield of polysaccharides as the index, the effects of amylase content, zymolytic time, extraction pH and zymolytic temperature were investigated. The optimal extraction conditions for polysaccharides were as follows: amylase content, 1% weight of pre-treated powder; zymolytic time, 2 h; extraction pH, 7.5; and zymolytic temperature, 55 °C. The yield was predicted to be 61.10%, which agreed with the value obtained in confirmatory experiments (59.71% ± 2.72%). Further research indicated that the primary component of E1P is glucose; however, it also contains a small quantity of galactose and arabinose. In vitro assays showed that E1P and ESBP (another kind of E. sibiricum bulb polysaccharide extracted by water decoction in our previous study) could significantly promote the cellular viability and phagocytosis of RAW264.7 cells without cytotoxicity. Moreover, they could enhance the ability to secrete nitric oxide and cytokines such as TNF-α and IL-1β. However, the immunomodulatory activities of E1P were better than those of ESBP. According to the results of this study, enzyme-assisted extraction represents a new strategy for extracting E. sibiricum bulb polysaccharides with higher yield and better immune activity.","PeriodicalId":12762,"journal":{"name":"Glycoconjugate Journal","volume":"39 3","pages":"357-368"},"PeriodicalIF":3.0,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39903099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Structural characteristics of Heparan sulfate required for the binding with the virus processing Enzyme Furin. 与病毒处理酶 Furin 结合所需的硫酸肝素的结构特征。

IF 3 4区生物学

Glycoconjugate Journal Pub Date : 2022-06-01 Epub Date: 2021-10-26 DOI: 10.1007/s10719-021-10018-8

Jiaxin Zeng, Yuan Meng, Shi-Yi Chen, Gaofeng Zhao, Lianchun Wang, En-Xin Zhang, Hong Qiu

{"title":"Structural characteristics of Heparan sulfate required for the binding with the virus processing Enzyme Furin.","authors":"Jiaxin Zeng, Yuan Meng, Shi-Yi Chen, Gaofeng Zhao, Lianchun Wang, En-Xin Zhang, Hong Qiu","doi":"10.1007/s10719-021-10018-8","DOIUrl":"10.1007/s10719-021-10018-8","url":null,"abstract":"Furin is one of the nine-member proprotein convertase family. Furin cleaves proteins with polybasic residues, which includes many viral glycoproteins such as SARS-Cov-2 spike protein. The cleavage is required for the activation of the proteins. Currently, the mechanisms that regulate Furin activity remain largely unknown. Here we demonstrated that Furin is a novel heparin/heparan sulfate binding protein by the use of biochemical and genetic assays. The KD is 9.78 nM based on the biolayer interferometry assay. Moreover, we found that sulfation degree, site-specific sulfation (N-sulfation and 3-O-sulfation), and iduronic acid are the major structural determinants for the binding. Furthermore, we found that heparin inhibits the enzymatic activity of Furin when pre-mixes heparin with either Furin or Furin substrate. We also found that the Furin binds with cells of different origin and the binding with the cells of lung origin is the strongest one. These data could advance our understanding of the working mechanism of Furin and will benefit the Furin based drug discovery such as inhibitors targeting the interaction between heparan sulfate and Furin for inhibition of viral infection.","PeriodicalId":12762,"journal":{"name":"Glycoconjugate Journal","volume":"39 3","pages":"315-325"},"PeriodicalIF":3.0,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8546381/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39558523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancement of Edwardsiella piscicida infection, biofilm formation, and motility caused by N-acetylneuraminate lyase. n -乙酰神经氨酸解酶增强鱼酸爱德华氏菌感染、生物膜形成和运动性。

IF 3 4区生物学

Glycoconjugate Journal Pub Date : 2022-06-01 Epub Date: 2022-02-22 DOI: 10.1007/s10719-022-10045-z

Linh Khanh Vo, Nhung Thi Tran, Yurina Kubo, Daichi Sahashi, Masaharu Komatsu, Kazuhiro Shiozaki

{"title":"Enhancement of Edwardsiella piscicida infection, biofilm formation, and motility caused by N-acetylneuraminate lyase.","authors":"Linh Khanh Vo, Nhung Thi Tran, Yurina Kubo, Daichi Sahashi, Masaharu Komatsu, Kazuhiro Shiozaki","doi":"10.1007/s10719-022-10045-z","DOIUrl":"https://doi.org/10.1007/s10719-022-10045-z","url":null,"abstract":"Sialic acid and its catabolism are involved in bacterial pathogenicity. N-acetylneuraminate lyase (NAL), which catalyzes the reversible aldol cleavage of sialic acid to form N-acetyl-D-mannosamine in the first step of sialic acid degradation, has been recently investigated to elucidate whether NAL enhances bacterial virulence; however, the role of NAL in bacterial pathogenicity remains unclear. In the present study, we demonstrated that the existence of two enzymes in Edwardsiella piscicida, referred to as dihydrodipicolinate synthase (DHDPS) and NAL, induced the cleavage/condensation activity toward sialic acids such as N-acetylneuraminic acid, N-glycolylneuraminic acid and 3-deoxy-D-glycero-D-galacto-non-2-ulopyranosonic acid. NAL enhanced cellular infection in vitro and suppressed the survival rate in zebrafish larvae in bath-infection in vivo, whereas DHDPS did not. Furthermore, NAL strongly activated the expression of E. piscicida phenotypes such as biofilm formation and motility, whereas DHDPS did not. Besides, the gene expression level of nanK, nanE, and glmU were up-regulated in the NAL-overexpressing strain, along with an increase in the total amount of N-acetylglucosamine.","PeriodicalId":12762,"journal":{"name":"Glycoconjugate Journal","volume":"39 3","pages":"429-442"},"PeriodicalIF":3.0,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39640616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Biosynthesis of the Pseudomonas aeruginosa common polysaccharide antigen by D-Rhamnosyltransferases WbpX and WbpY. d -鼠李糖基转移酶WbpX和WbpY合成铜绿假单胞菌多糖共同抗原

IF 3 4区生物学

Glycoconjugate Journal Pub Date : 2022-06-01 Epub Date: 2022-02-15 DOI: 10.1007/s10719-022-10040-4

Jacob Melamed, Alexander Kocev, Vladimir Torgov, Vladimir Veselovsky, Inka Brockhausen

{"title":"Biosynthesis of the Pseudomonas aeruginosa common polysaccharide antigen by D-Rhamnosyltransferases WbpX and WbpY.","authors":"Jacob Melamed, Alexander Kocev, Vladimir Torgov, Vladimir Veselovsky, Inka Brockhausen","doi":"10.1007/s10719-022-10040-4","DOIUrl":"https://doi.org/10.1007/s10719-022-10040-4","url":null,"abstract":"The Gram-negative bacterium Pseudomonas aeruginosa simultaneously expresses two O-antigenic glycoforms. While the O-specific antigen (OSA) is variable in composition, the common polysaccharide antigen (CPA) is highly conserved and is composed of a homopolymer of D-rhamnose (D-Rha) in trisaccharide repeating units [D-Rhaα1-2-D-Rhaα1-3-D-Rhaɑ1-3]n. We have previously reported that α3-D-Rha-transferase WbpZ transfers a D-Rha residue from GDP-D-Rha to D-GlcNAcα-O-PO3-PO3-(CH2)11-O-phenyl. Genes encoding two more D-Rha-transferases are found in the O antigen gene cluster (wbpX and wbpY). In this study we showed that WbpX and WbpY recombinantly expressed in E. coli differ in their donor and acceptor specificities and have properties of GT-B folded enzymes of the GT4 glycosyltransferase family. NMR spectroscopic analysis of the WbpY reaction product showed that WbpY transferred one D-Rha residue in α1-3 linkage to synthetic D-Rhaα1-3-D-GlcNAcα-O-PO3-PO3-(CH2)11-O-phenyl acceptor. WbpX synthesized several products that contained D-Rha in both α1-2 and α1-3 linkages. Mass spectrometry indicated that the mixture of WbpX and WbpY efficiently catalyzed the synthesis of D-Rha oligomers in a non-processive mechanism. Since O antigens are virulence factors, these findings open the door to advancing technology for antibacterial drug discovery and vaccine development.","PeriodicalId":12762,"journal":{"name":"Glycoconjugate Journal","volume":"39 3","pages":"393-411"},"PeriodicalIF":3.0,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8853325/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39787383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3