Glycoconjugate Journal最新文献

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The story of the Sda antigen and of its cognate enzyme B4GALNT2: What is new? Sda 抗原及其同源酶 B4GALNT2 的故事:有什么新发现?
IF 2.7 4区 生物学
Glycoconjugate Journal Pub Date : 2023-02-01 Epub Date: 2022-10-26 DOI: 10.1007/s10719-022-10089-1
Martina Duca, Nadia Malagolini, Fabio Dall'Olio
{"title":"The story of the Sd<sup>a</sup> antigen and of its cognate enzyme B4GALNT2: What is new?","authors":"Martina Duca, Nadia Malagolini, Fabio Dall'Olio","doi":"10.1007/s10719-022-10089-1","DOIUrl":"10.1007/s10719-022-10089-1","url":null,"abstract":"<p><p>The structure Siaα2,3(GalNAcβ1,4)Gal- is the epitope of the Sd<sup>a</sup> antigen, which is expressed on the erythrocytes and secretions of the vast majority of Caucasians, carried by N- and O-linked chains of glycoproteins, as well as by glycolipids. Sd<sup>a</sup> is very similar, but not identical, to ganglioside GM2 [Siaα2,3(GalNAcβ1,4)Galβ1,4Glc-Cer]. The Sd<sup>a</sup> synthase β1,4 N-acetylgalactosaminyl transferase 2 (B4GALNT2) exists in a short and a long form, diverging in the aminoterminal domain. The latter has a very long cytoplasmic tail and displays a Golgi- as well as a post-Golgi localization. The biosynthesis of Sd<sup>a</sup> is mutually exclusive with that of the cancer-associated sialyl Lewis antigens, whose structure is Siaα2,3Galβ1,3/4(Fucα1,4/3)GlcNAc-. B4GALNT2 is down-regulated in colon cancer but patients with higher expression survive longer. In experimental systems, B4GALNT2 inhibits colon cancer progression,not only through inhibition of sialyl Lewis antigen biosynthesis. By contrast, in breast cancer B4GALNT2 is associated with malignancy. In colon cancer, the B4GALNT2 gene is regulated by multiple mechanisms, which include miRNA and transcription factor expression, as well as CpG methylation. In addition, Sd<sup>a</sup>/B4GALNT2 regulates the susceptibility to infectious agents, the protection from muscle dystrophy, the activity of immune system in pregnancy and the immune rejection in xenotransplantation.</p>","PeriodicalId":12762,"journal":{"name":"Glycoconjugate Journal","volume":"40 1","pages":"123-133"},"PeriodicalIF":2.7,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10730559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Plant lectins as versatile tools to fight coronavirus outbreaks. 植物凝集素是抗击冠状病毒爆发的多功能工具。
IF 2.7 4区 生物学
Glycoconjugate Journal Pub Date : 2023-02-01 Epub Date: 2022-11-24 DOI: 10.1007/s10719-022-10094-4
Mathias Simplicien, Pierre Pério, Jan Sudor, Annick Barre, Hervé Benoist, Els J M Van Damme, Pierre Rougé
{"title":"Plant lectins as versatile tools to fight coronavirus outbreaks.","authors":"Mathias Simplicien, Pierre Pério, Jan Sudor, Annick Barre, Hervé Benoist, Els J M Van Damme, Pierre Rougé","doi":"10.1007/s10719-022-10094-4","DOIUrl":"10.1007/s10719-022-10094-4","url":null,"abstract":"<p><p>The S protein forming the homotrimeric spikes of pathogenic beta-coronaviruses, such as MERS-CoV, SARS-CoV and SARS-CoV-2, is a highly glycosylated protein containing mainly N-glycans of the complex and high-mannose type, as well as O-glycans. Similarly, the host cell receptors DPP4 for MERS-CoV and ACE2 for SARS-CoV and SARS-CoV-2, also represent N- and O-glycosylated proteins. All these glycoproteins share common glycosylation patterns, suggesting that plant lectins with different carbohydrate-binding specificities could be used as carbohydrate-binding agents for the spikes and their receptors, to combat COVID19 pandemics. The binding of plant lectins to the spikes and their receptors could mask the non-glycosylated receptor binding domain of the virus and the corresponding region of the receptor, thus preventing a proper interaction of the spike proteins with their receptors. In this review, we analyze (1) the ability of plant lectins to interact with the N- and O-glycans present on the spike proteins and their receptors, (2) the in vitro and in vivo anti-COVID19 activity already reported for plant lectins and, (3) the possible ways for delivery of lectins to block the spikes and/or their receptors.</p>","PeriodicalId":12762,"journal":{"name":"Glycoconjugate Journal","volume":"40 1","pages":"109-118"},"PeriodicalIF":2.7,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9684959/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10731103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Pseudomonas syringae DC3000 infection increases glucosylated N-glycans in Arabidopsis thaliana. 拟南芥中的丁香假单胞菌 DC3000 感染会增加葡萄糖基化的 N-聚糖。
IF 2.7 4区 生物学
Glycoconjugate Journal Pub Date : 2023-02-01 Epub Date: 2022-10-21 DOI: 10.1007/s10719-022-10084-6
Gernot Beihammer, Andrea Romero-Pérez, Daniel Maresch, Rudolf Figl, Réka Mócsai, Clemens Grünwald-Gruber, Friedrich Altmann, Els J M Van Damme, Richard Strasser
{"title":"Pseudomonas syringae DC3000 infection increases glucosylated N-glycans in Arabidopsis thaliana.","authors":"Gernot Beihammer, Andrea Romero-Pérez, Daniel Maresch, Rudolf Figl, Réka Mócsai, Clemens Grünwald-Gruber, Friedrich Altmann, Els J M Van Damme, Richard Strasser","doi":"10.1007/s10719-022-10084-6","DOIUrl":"10.1007/s10719-022-10084-6","url":null,"abstract":"<p><p>Studying the interaction between the hemibiotrophic bacterium Pseudomonas syringae pv. tomato DC3000 and Arabidopsis thaliana has shed light onto the various forms of mechanisms plants use to defend themselves against pathogen attack. While a lot of emphasis has been put on investigating changes in protein expression in infected plants, only little information is available on the effect infection plays on the plants N-glycan composition. To close this gap in knowledge, total N-glycans were enriched from P. syringae DC3000-infected and mock treated Arabidopsis seedlings and analyzed via MALDI-TOF-MS. Additionally, fluorescently labelled N-glycans were quantified via HPLC-FLD. N-glycans from infected plants were overall less processed and displayed increased amounts of oligomannosidic N-glycans. As multiple peaks for certain oligomannosidic glycoforms were detected upon separation via liquid chromatography, a porous graphitic carbon (PGC)-analysis was conducted to separate individual N-glycan isomers. Indeed, multiple different N-glycan isomers with masses of two N-acetylhexosamine residues plus 8, 9 or 10 hexoses were detected in the infected plants which were absent in the mock controls. Treatment with jack bean α-mannosidase resulted in incomplete removal of hexoses from these N-glycans, indicating the presence of glucose residues. This hints at the accumulation of misfolded glycoproteins in the infected plants, likely because of endoplasmic reticulum (ER) stress. In addition, poly-hexose structures susceptible to α-amylase treatment were found in the DC3000-infected plants, indicating alterations in starch metabolism due to the infection process.</p>","PeriodicalId":12762,"journal":{"name":"Glycoconjugate Journal","volume":"40 1","pages":"97-108"},"PeriodicalIF":2.7,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9925501/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10734654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Afterword (Editorial). 后记(社论)。
IF 2.7 4区 生物学
Glycoconjugate Journal Pub Date : 2023-02-01 Epub Date: 2022-11-02 DOI: 10.1007/s10719-022-10090-8
Kazuhisa Iwabuchi, Alessandro Prinetti
{"title":"Afterword (Editorial).","authors":"Kazuhisa Iwabuchi, Alessandro Prinetti","doi":"10.1007/s10719-022-10090-8","DOIUrl":"10.1007/s10719-022-10090-8","url":null,"abstract":"","PeriodicalId":12762,"journal":{"name":"Glycoconjugate Journal","volume":"40 1","pages":"119-122"},"PeriodicalIF":2.7,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10730569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Partial characterization of purified glycoprotein from nutshell of Arachis hypogea L. towards macrophage activation and leishmaniacidal activity. 从榛子果壳中纯化的糖蛋白对巨噬细胞活化和利什曼杀虫活性的部分表征。
IF 2.7 4区 生物学
Glycoconjugate Journal Pub Date : 2023-02-01 Epub Date: 2023-01-03 DOI: 10.1007/s10719-022-10096-2
Sujatha Srinivasan, Mamilla R Charan Raja, Amrita Kar, Aishwarya Ramasamy, Adithyan Jayaraman, Vellingiri Vadivel, Santanu Kar Mahapatra
{"title":"Partial characterization of purified glycoprotein from nutshell of Arachis hypogea L. towards macrophage activation and leishmaniacidal activity.","authors":"Sujatha Srinivasan, Mamilla R Charan Raja, Amrita Kar, Aishwarya Ramasamy, Adithyan Jayaraman, Vellingiri Vadivel, Santanu Kar Mahapatra","doi":"10.1007/s10719-022-10096-2","DOIUrl":"10.1007/s10719-022-10096-2","url":null,"abstract":"<p><p>Arachis hypogea L. protein fraction-2 (AHP-F2) from the Peanut shell was extracted and characterized and its potent immunomodulatory and anti-leishmanial role was determined in this present study. AHP-F2 was found to be a glycoprotein as the presence of carbohydrates were confirmed by the analysis of high-performance liquid chromatography (HPLC) yielded glucose, galactose, mannose, and xylose. AHP-F2 molecular mass was found to be ∼28 kDa as indicated in MALDI-TOF and peptide mass fingerprinting analysis followed by Mascot search. The peptide matches revealed the similarity of the mannose/glucose binding lectin with 71.07% in the BLAST analysis. After that, the 3D structure of the AHP-F2 model was designed and validated by the Ramachandran plot. The immunomodulatory role of AHP-F2 was established in murine peritoneal macrophages as induction of nitric oxide (NO), and stimulation of proinflammatory cytokines (IL-12 and IFN-γ) in a dose-dependent manner was observed. Interestingly, it was also found that AHP-F2 has interacted with the innate immune receptor, toll-like receptors (TLRs) as established in molecular docking as well as mRNA expression. The anti-leishmanial potential of AHP-F2 was revealed with a prominent inhibition of amastigote growth within the murine macrophages with prompt induction of nitrite release. Altogether, the isolated AHP-F2 from Arachis hypogea L. has strong immunomodulatory and anti-leishmanial potential which may disclose a new path to treat leishmaniasis.</p>","PeriodicalId":12762,"journal":{"name":"Glycoconjugate Journal","volume":"40 1","pages":"1-17"},"PeriodicalIF":2.7,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10736164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A galactoside-specific Dalbergieae legume lectin from seeds of Vataireopsis araroba (Aguiar) Ducke. 一种来自 Vataireopsis araroba (Aguiar) Ducke 种子的半乳糖苷特异性豆科凝集素。
IF 2.7 4区 生物学
Glycoconjugate Journal Pub Date : 2023-02-01 Epub Date: 2022-10-26 DOI: 10.1007/s10719-022-10082-8
Vinicius J S Osterne, Messias V Oliveira, Kristof De Schutter, Sonia Serna, Niels-Christian Reichardt, Guy Smagghe, Benildo S Cavada, Els J M Van Damme, Kyria Santiago Nascimento
{"title":"A galactoside-specific Dalbergieae legume lectin from seeds of Vataireopsis araroba (Aguiar) Ducke.","authors":"Vinicius J S Osterne, Messias V Oliveira, Kristof De Schutter, Sonia Serna, Niels-Christian Reichardt, Guy Smagghe, Benildo S Cavada, Els J M Van Damme, Kyria Santiago Nascimento","doi":"10.1007/s10719-022-10082-8","DOIUrl":"10.1007/s10719-022-10082-8","url":null,"abstract":"<p><p>The Dalbergieae lectin group encompasses several lectins with significant differences in their carbohydrate specificities and biological properties. The current work reports on the purification and characterization of a GalNAc/Gal-specific lectin from Vataireopsis araroba (Aguiar) Ducke, designated as VaL. The lectin was purified from the seeds in a single step using guar gum affinity chromatography. The lectin migrated as a single band of about 35 kDa on SDS-PAGE and, in native conditions, occurs as a homodimer. The purified lectin is stable at temperatures up to 60 °C and in a pH range from 7 to 8 and requires divalent cations for its activity. Sugar-inhibition assays demonstrate the lectin specificity towards N-acetyl-D-galactosamine, D-galactose and related sugars. Furthermore, glycan array analyses show that VaL interacts preferentially with glycans containing terminal GalNAc/Galβ1-4GlcNAc. Biological activity assays were performed using three insect cell lines: CF1 midgut cells from the spruce budworm Choristoneura fumiferana, S2 embryo cells from the fruit fly Drosophila melanogaster, and GutAW midgut cells from the corn earworm Helicoverpa zea. In vitro assays indicated a biostatic effect for VaL on CF1 cells, but not on S2 and GutAW cells. The lectin presented a biostatic effect by reducing the cell growth and inducing cell agglutination, suggesting an interaction with glycans on the cell surface. VaL has been characterized as a galactoside-specific lectin of the Dalbergieae tribe, with sequence similarity to lectins from Vatairea and Arachis.</p>","PeriodicalId":12762,"journal":{"name":"Glycoconjugate Journal","volume":"40 1","pages":"85-95"},"PeriodicalIF":2.7,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10718466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Phosphorylated chitosan accelerates dermal wound healing in diabetic wistar rats. 磷酸化壳聚糖可加速糖尿病wistar大鼠真皮伤口的愈合。
IF 2.7 4区 生物学
Glycoconjugate Journal Pub Date : 2023-02-01 Epub Date: 2022-11-30 DOI: 10.1007/s10719-022-10093-5
U Anushree, Pratik Punj, Vasumathi, Sanjay Bharati
{"title":"Phosphorylated chitosan accelerates dermal wound healing in diabetic wistar rats.","authors":"U Anushree, Pratik Punj, Vasumathi, Sanjay Bharati","doi":"10.1007/s10719-022-10093-5","DOIUrl":"10.1007/s10719-022-10093-5","url":null,"abstract":"<p><p>Phosphorylated chitosan (PC), a water-soluble derivative of chitosan possesses several biological and chemical properties suitable for diabetic wound healing. In the present study, we report the synthesis and diabetic wound healing capabilities of PC. Elemental analysis, FT-IR, <sup>13</sup>C-NMR and <sup>31</sup>P-NMR techniques were employed for the chemical characterization of PC. In vitro, antioxidant properties of PC were determined in terms of Fe<sup>3+</sup> reducing, metal chelating, lipid peroxidation and superoxide scavenging ability. The wound healing potential of PC was assessed in diabetic excisional wound rat model. PC exhibited good water solubility, and in vitro antioxidant capacity. Wound contraction was higher in PC-treated wounds (91.11%) as compared to untreated wounds (67.26%) on 14<sup>th</sup>-day post wound creation. Histopathology of PC-treated wounds revealed improved tissue morphology with higher number of fibroblasts, a thicker epithelial layer, enhanced collagen deposits and angiogenesis as compared to untreated wounds. An overall increase of 57% and 25% in hydroxylamine and hexosamine content respectively were noted as compared to untreated wounds. A significant (P ≤ 0.05) increase in SOD activity and a significant (P ≤ 0.05) decrease in lipid peroxides were recorded in PC-treated wounds as compared to untreated wounds. These observations demonstrated that PC can be used as an effective agent in diabetic wound healing. Illustration of phosphorylated chitosan (PC) synthesis and its wound healing potential: Chitosan was phosphorylated to impart diabetic wound healing properties. Chemical characterizations such as elemental analysis, FT-IR and NMR confirmed successful phosphorylation of chitosan. PC exhibited good in vitro antioxidant properties. To assess the diabetic wound healing potential, an excisional wound model was developed in diabetic rats. PC treatment demonstrated accelerated wound healing.</p>","PeriodicalId":12762,"journal":{"name":"Glycoconjugate Journal","volume":"40 1","pages":"19-31"},"PeriodicalIF":2.7,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9925528/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10736426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Structural characterization and biological activity of an α-glucan from the mollusk Marcia hiantina (Lamarck, 1818). 来自软体动物 Marcia hiantina (Lamarck, 1818) 的 α-葡聚糖的结构特征和生物活性。
IF 2.7 4区 生物学
Glycoconjugate Journal Pub Date : 2023-02-01 Epub Date: 2022-12-01 DOI: 10.1007/s10719-022-10092-6
Hoda Al Ahmed, Bernadeth F Ticar, Ian Black, Fakhri Mahdi, Anter A Shami, Sandeep K Misra, Christian Heiss, Jason J Paris, Joshua S Sharp, Parastoo Azadi, Vitor H Pomin
{"title":"Structural characterization and biological activity of an α-glucan from the mollusk Marcia hiantina (Lamarck, 1818).","authors":"Hoda Al Ahmed, Bernadeth F Ticar, Ian Black, Fakhri Mahdi, Anter A Shami, Sandeep K Misra, Christian Heiss, Jason J Paris, Joshua S Sharp, Parastoo Azadi, Vitor H Pomin","doi":"10.1007/s10719-022-10092-6","DOIUrl":"10.1007/s10719-022-10092-6","url":null,"abstract":"<p><p>Marcia hiantina (Mollusca, Bivalvia) (Lamarck, 1818), is an edible clam mainly distributed along the tropical coastal regions. Recent researches have demonstrated that clams can possess compounds, including polysaccharides, with a wide range of biological actions including antioxidant, immunomodulatory and antitumor activities. Here an α-glucan was isolated from M. hiantina by hot water, purified by anion exchange chromatography, and its structure was characterized by a combination of multiple nuclear magnetic resonance (NMR) methods (1D <sup>1</sup>H, <sup>1</sup>H-<sup>1</sup>H COSY, <sup>1</sup>H-<sup>1</sup>H TOCSY, <sup>1</sup>H-<sup>1</sup>H NOESY, <sup>1</sup>H-<sup>13</sup>C HSQC and <sup>1</sup>H-<sup>13</sup>C HSQC-NOESY spectra), gas chromatography-mass spectrometry, and high performance size exclusion chromatography (HPSEC). The analysis from NMR, monosaccharide composition, methylation analyses and HPSEC combined with multi-angle light scattering (MALS) of M. hiantina-derived α-glycan confirmed a branched polysaccharide exclusively composed of glucose (Glc), mostly 4-linked in its backbone, branched occasionally at 6-positions, and having a molecular weight of ~ 570 kDa. The mollusk α-glucan was subjected to four cell-based assays: (i) viability of three cell lines (RAW264.7, HaCaT, and HT-29), (ii) activity on lipopolysaccharide (LPS)-induced prostaglandin production in RAW264.7 cells, (iii) inhibitory activities of in H<sub>2</sub>O<sub>2</sub>- and LPS-induced reactive oxygen species (ROS) production in HMC3 cells, and (iv) HaCaT cell proliferation. Results have indicated no cytotoxicity, potent inhibition of both H<sub>2</sub>O<sub>2</sub>- and LPS-induced ROS, and potent cell proliferative activity.</p>","PeriodicalId":12762,"journal":{"name":"Glycoconjugate Journal","volume":"40 1","pages":"33-46"},"PeriodicalIF":2.7,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9931679/pdf/nihms-1868113.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10790939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
6-O-desulfated heparin attenuates myocardial ischemia/reperfusion injury in mice through the regulation of miR-199a-5p/klotho axis. 6- o -去硫肝素通过调控miR-199a-5p/klotho轴减轻小鼠心肌缺血/再灌注损伤。
IF 3 4区 生物学
Glycoconjugate Journal Pub Date : 2022-12-01 DOI: 10.1007/s10719-022-10081-9
Yujie Wang, Ting Li, Niansheng Li, Chuyi Huang, Xiaoming Xiong, Xu Xie, Meiting Wu, Lianchun Wang, Junlin Jiang
{"title":"6-O-desulfated heparin attenuates myocardial ischemia/reperfusion injury in mice through the regulation of miR-199a-5p/klotho axis.","authors":"Yujie Wang,&nbsp;Ting Li,&nbsp;Niansheng Li,&nbsp;Chuyi Huang,&nbsp;Xiaoming Xiong,&nbsp;Xu Xie,&nbsp;Meiting Wu,&nbsp;Lianchun Wang,&nbsp;Junlin Jiang","doi":"10.1007/s10719-022-10081-9","DOIUrl":"https://doi.org/10.1007/s10719-022-10081-9","url":null,"abstract":"<p><p>Heparin has been documented to reduce myocardial injury caused by ischemia/reperfusion (I/R), but its clinical application is limited due to its strong intrinsic anticoagulant property. Some desulfated derivatives of heparin display low anticoagulant activity and may have potential value as therapeutic agents for myocardial I/R injury. In this study, we observed that 6-O-desulfated heparin, a desulfated derivative of heparin, shortened the activated partial thromboplastin time and exhibited lower anticoagulant activity compared with heparin or 2-O-desulfated heparin (another desulfated derivative of heparin). Then, we explored whether 6-O-desulfated heparin could protect against myocardial I/R injury, and elucidated its possible mechanisms. Administration of 6-O-desulfated heparin significantly reduced creatine kinase activity, myocardial infarct size and cell apoptosis in mice subjected to 30 min of myocardial ischemia following 2 h of reperfusion, accompanied by a reverse in miR-199a-5p elevation, klotho downregulation and reactive oxygen species (ROS) accumulation. In cultured H9c2 cells, the mechanism of 6-O-desulfated heparin against myocardial I/R injury was further explored. Consistent with the results in vivo, 6-O-desulfated heparin significantly ameliorated hypoxia/reoxygenation-induced injury, upregulated klotho and decreased miR-199a-5p levels and ROS accumulation, and these effects were reversed by miR-199a-5p mimics. In conclusion, these results suggested that 6-O-desulfated heparin with lower anticoagulant activity attenuated myocardial I/R injury through miR-199a-5p/klotho and ROS signaling. Our study may also indicate that 6-O-desulfated heparin, as an excellent heparin derivative, is a potential therapeutic agent for myocardial I/R injury.</p>","PeriodicalId":12762,"journal":{"name":"Glycoconjugate Journal","volume":"39 6","pages":"747-758"},"PeriodicalIF":3.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10051514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Envelope Glycoprotein based multi-epitope vaccine against a co-infection of Human Herpesvirus 5 and Human Herpesvirus 6 using in silico strategies. 基于包膜糖蛋白的抗人疱疹病毒5型和人疱疹病毒6型合并感染多表位疫苗
IF 3 4区 生物学
Glycoconjugate Journal Pub Date : 2022-12-01 DOI: 10.1007/s10719-022-10083-7
Bharath Sai Gandhamaneni, HemaNandini Rajendran Krishnamoorthy, Shanthi Veerappapillai, Soumya R Mohapatra, Ramanathan Karuppasamy
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