Glycoconjugate Journal最新文献_第9页

A galactoside-specific Dalbergieae legume lectin from seeds of Vataireopsis araroba (Aguiar) Ducke. 一种来自 Vataireopsis araroba (Aguiar) Ducke 种子的半乳糖苷特异性豆科凝集素。

IF 2.7 4区生物学

Glycoconjugate Journal Pub Date : 2023-02-01 Epub Date: 2022-10-26 DOI: 10.1007/s10719-022-10082-8

Vinicius J S Osterne, Messias V Oliveira, Kristof De Schutter, Sonia Serna, Niels-Christian Reichardt, Guy Smagghe, Benildo S Cavada, Els J M Van Damme, Kyria Santiago Nascimento

{"title":"A galactoside-specific Dalbergieae legume lectin from seeds of Vataireopsis araroba (Aguiar) Ducke.","authors":"Vinicius J S Osterne, Messias V Oliveira, Kristof De Schutter, Sonia Serna, Niels-Christian Reichardt, Guy Smagghe, Benildo S Cavada, Els J M Van Damme, Kyria Santiago Nascimento","doi":"10.1007/s10719-022-10082-8","DOIUrl":"10.1007/s10719-022-10082-8","url":null,"abstract":"The Dalbergieae lectin group encompasses several lectins with significant differences in their carbohydrate specificities and biological properties. The current work reports on the purification and characterization of a GalNAc/Gal-specific lectin from Vataireopsis araroba (Aguiar) Ducke, designated as VaL. The lectin was purified from the seeds in a single step using guar gum affinity chromatography. The lectin migrated as a single band of about 35 kDa on SDS-PAGE and, in native conditions, occurs as a homodimer. The purified lectin is stable at temperatures up to 60 °C and in a pH range from 7 to 8 and requires divalent cations for its activity. Sugar-inhibition assays demonstrate the lectin specificity towards N-acetyl-D-galactosamine, D-galactose and related sugars. Furthermore, glycan array analyses show that VaL interacts preferentially with glycans containing terminal GalNAc/Galβ1-4GlcNAc. Biological activity assays were performed using three insect cell lines: CF1 midgut cells from the spruce budworm Choristoneura fumiferana, S2 embryo cells from the fruit fly Drosophila melanogaster, and GutAW midgut cells from the corn earworm Helicoverpa zea. In vitro assays indicated a biostatic effect for VaL on CF1 cells, but not on S2 and GutAW cells. The lectin presented a biostatic effect by reducing the cell growth and inducing cell agglutination, suggesting an interaction with glycans on the cell surface. VaL has been characterized as a galactoside-specific lectin of the Dalbergieae tribe, with sequence similarity to lectins from Vatairea and Arachis.","PeriodicalId":12762,"journal":{"name":"Glycoconjugate Journal","volume":"40 1","pages":"85-95"},"PeriodicalIF":2.7,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10718466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phosphorylated chitosan accelerates dermal wound healing in diabetic wistar rats. 磷酸化壳聚糖可加速糖尿病wistar大鼠真皮伤口的愈合。

IF 2.7 4区生物学

Glycoconjugate Journal Pub Date : 2023-02-01 Epub Date: 2022-11-30 DOI: 10.1007/s10719-022-10093-5

U Anushree, Pratik Punj, Vasumathi, Sanjay Bharati

{"title":"Phosphorylated chitosan accelerates dermal wound healing in diabetic wistar rats.","authors":"U Anushree, Pratik Punj, Vasumathi, Sanjay Bharati","doi":"10.1007/s10719-022-10093-5","DOIUrl":"10.1007/s10719-022-10093-5","url":null,"abstract":"Phosphorylated chitosan (PC), a water-soluble derivative of chitosan possesses several biological and chemical properties suitable for diabetic wound healing. In the present study, we report the synthesis and diabetic wound healing capabilities of PC. Elemental analysis, FT-IR, 13C-NMR and 31P-NMR techniques were employed for the chemical characterization of PC. In vitro, antioxidant properties of PC were determined in terms of Fe3+ reducing, metal chelating, lipid peroxidation and superoxide scavenging ability. The wound healing potential of PC was assessed in diabetic excisional wound rat model. PC exhibited good water solubility, and in vitro antioxidant capacity. Wound contraction was higher in PC-treated wounds (91.11%) as compared to untreated wounds (67.26%) on 14th-day post wound creation. Histopathology of PC-treated wounds revealed improved tissue morphology with higher number of fibroblasts, a thicker epithelial layer, enhanced collagen deposits and angiogenesis as compared to untreated wounds. An overall increase of 57% and 25% in hydroxylamine and hexosamine content respectively were noted as compared to untreated wounds. A significant (P ≤ 0.05) increase in SOD activity and a significant (P ≤ 0.05) decrease in lipid peroxides were recorded in PC-treated wounds as compared to untreated wounds. These observations demonstrated that PC can be used as an effective agent in diabetic wound healing. Illustration of phosphorylated chitosan (PC) synthesis and its wound healing potential: Chitosan was phosphorylated to impart diabetic wound healing properties. Chemical characterizations such as elemental analysis, FT-IR and NMR confirmed successful phosphorylation of chitosan. PC exhibited good in vitro antioxidant properties. To assess the diabetic wound healing potential, an excisional wound model was developed in diabetic rats. PC treatment demonstrated accelerated wound healing.","PeriodicalId":12762,"journal":{"name":"Glycoconjugate Journal","volume":"40 1","pages":"19-31"},"PeriodicalIF":2.7,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9925528/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10736426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structural characterization and biological activity of an α-glucan from the mollusk Marcia hiantina (Lamarck, 1818). 来自软体动物 Marcia hiantina (Lamarck, 1818) 的 α-葡聚糖的结构特征和生物活性。

IF 2.7 4区生物学

Glycoconjugate Journal Pub Date : 2023-02-01 Epub Date: 2022-12-01 DOI: 10.1007/s10719-022-10092-6

Hoda Al Ahmed, Bernadeth F Ticar, Ian Black, Fakhri Mahdi, Anter A Shami, Sandeep K Misra, Christian Heiss, Jason J Paris, Joshua S Sharp, Parastoo Azadi, Vitor H Pomin

{"title":"Structural characterization and biological activity of an α-glucan from the mollusk Marcia hiantina (Lamarck, 1818).","authors":"Hoda Al Ahmed, Bernadeth F Ticar, Ian Black, Fakhri Mahdi, Anter A Shami, Sandeep K Misra, Christian Heiss, Jason J Paris, Joshua S Sharp, Parastoo Azadi, Vitor H Pomin","doi":"10.1007/s10719-022-10092-6","DOIUrl":"10.1007/s10719-022-10092-6","url":null,"abstract":"Marcia hiantina (Mollusca, Bivalvia) (Lamarck, 1818), is an edible clam mainly distributed along the tropical coastal regions. Recent researches have demonstrated that clams can possess compounds, including polysaccharides, with a wide range of biological actions including antioxidant, immunomodulatory and antitumor activities. Here an α-glucan was isolated from M. hiantina by hot water, purified by anion exchange chromatography, and its structure was characterized by a combination of multiple nuclear magnetic resonance (NMR) methods (1D 1H, 1H-1H COSY, 1H-1H TOCSY, 1H-1H NOESY, 1H-13C HSQC and 1H-13C HSQC-NOESY spectra), gas chromatography-mass spectrometry, and high performance size exclusion chromatography (HPSEC). The analysis from NMR, monosaccharide composition, methylation analyses and HPSEC combined with multi-angle light scattering (MALS) of M. hiantina-derived α-glycan confirmed a branched polysaccharide exclusively composed of glucose (Glc), mostly 4-linked in its backbone, branched occasionally at 6-positions, and having a molecular weight of ~ 570 kDa. The mollusk α-glucan was subjected to four cell-based assays: (i) viability of three cell lines (RAW264.7, HaCaT, and HT-29), (ii) activity on lipopolysaccharide (LPS)-induced prostaglandin production in RAW264.7 cells, (iii) inhibitory activities of in H2O2- and LPS-induced reactive oxygen species (ROS) production in HMC3 cells, and (iv) HaCaT cell proliferation. Results have indicated no cytotoxicity, potent inhibition of both H2O2- and LPS-induced ROS, and potent cell proliferative activity.","PeriodicalId":12762,"journal":{"name":"Glycoconjugate Journal","volume":"40 1","pages":"33-46"},"PeriodicalIF":2.7,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9931679/pdf/nihms-1868113.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10790939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

6-O-desulfated heparin attenuates myocardial ischemia/reperfusion injury in mice through the regulation of miR-199a-5p/klotho axis. 6- o -去硫肝素通过调控miR-199a-5p/klotho轴减轻小鼠心肌缺血/再灌注损伤。

IF 3 4区生物学

Glycoconjugate Journal Pub Date : 2022-12-01 DOI: 10.1007/s10719-022-10081-9

Yujie Wang, Ting Li, Niansheng Li, Chuyi Huang, Xiaoming Xiong, Xu Xie, Meiting Wu, Lianchun Wang, Junlin Jiang

{"title":"6-O-desulfated heparin attenuates myocardial ischemia/reperfusion injury in mice through the regulation of miR-199a-5p/klotho axis.","authors":"Yujie Wang, Ting Li, Niansheng Li, Chuyi Huang, Xiaoming Xiong, Xu Xie, Meiting Wu, Lianchun Wang, Junlin Jiang","doi":"10.1007/s10719-022-10081-9","DOIUrl":"https://doi.org/10.1007/s10719-022-10081-9","url":null,"abstract":"Heparin has been documented to reduce myocardial injury caused by ischemia/reperfusion (I/R), but its clinical application is limited due to its strong intrinsic anticoagulant property. Some desulfated derivatives of heparin display low anticoagulant activity and may have potential value as therapeutic agents for myocardial I/R injury. In this study, we observed that 6-O-desulfated heparin, a desulfated derivative of heparin, shortened the activated partial thromboplastin time and exhibited lower anticoagulant activity compared with heparin or 2-O-desulfated heparin (another desulfated derivative of heparin). Then, we explored whether 6-O-desulfated heparin could protect against myocardial I/R injury, and elucidated its possible mechanisms. Administration of 6-O-desulfated heparin significantly reduced creatine kinase activity, myocardial infarct size and cell apoptosis in mice subjected to 30 min of myocardial ischemia following 2 h of reperfusion, accompanied by a reverse in miR-199a-5p elevation, klotho downregulation and reactive oxygen species (ROS) accumulation. In cultured H9c2 cells, the mechanism of 6-O-desulfated heparin against myocardial I/R injury was further explored. Consistent with the results in vivo, 6-O-desulfated heparin significantly ameliorated hypoxia/reoxygenation-induced injury, upregulated klotho and decreased miR-199a-5p levels and ROS accumulation, and these effects were reversed by miR-199a-5p mimics. In conclusion, these results suggested that 6-O-desulfated heparin with lower anticoagulant activity attenuated myocardial I/R injury through miR-199a-5p/klotho and ROS signaling. Our study may also indicate that 6-O-desulfated heparin, as an excellent heparin derivative, is a potential therapeutic agent for myocardial I/R injury.","PeriodicalId":12762,"journal":{"name":"Glycoconjugate Journal","volume":"39 6","pages":"747-758"},"PeriodicalIF":3.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10051514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Envelope Glycoprotein based multi-epitope vaccine against a co-infection of Human Herpesvirus 5 and Human Herpesvirus 6 using in silico strategies. 基于包膜糖蛋白的抗人疱疹病毒5型和人疱疹病毒6型合并感染多表位疫苗

IF 3 4区生物学

Glycoconjugate Journal Pub Date : 2022-12-01 DOI: 10.1007/s10719-022-10083-7

Bharath Sai Gandhamaneni, HemaNandini Rajendran Krishnamoorthy, Shanthi Veerappapillai, Soumya R Mohapatra, Ramanathan Karuppasamy

{"title":"Envelope Glycoprotein based multi-epitope vaccine against a co-infection of Human Herpesvirus 5 and Human Herpesvirus 6 using in silico strategies.","authors":"Bharath Sai Gandhamaneni, HemaNandini Rajendran Krishnamoorthy, Shanthi Veerappapillai, Soumya R Mohapatra, Ramanathan Karuppasamy","doi":"10.1007/s10719-022-10083-7","DOIUrl":"https://doi.org/10.1007/s10719-022-10083-7","url":null,"abstract":"The Human Betaherpesviruses HHV-5 and HHV-6 are quite inimical in immunocompromised hosts individually. A co-infection of both has been surmised to be far more disastrous. This can be attributed to a synergetic effect of their combined pathologies. While there have been attempts to develop a vaccine against each virus, no efforts were made to contrive an effective prophylaxis for the highly detrimental co-infection. In this study, an ensemble of viral envelope glycoproteins from both the viruses was utilized to design a multi-epitope vaccine using immunoinformatics tools. A collection of bacterial protein toll-like receptor agonists (BPTAs) was screened to identify a highly immunogenic adjuvant for the vaccine construct. The constructed vaccine was analysed using an array of methodologies ranging from World population coverage analysis to Immune simulation, whose results indicate high vaccine efficacy and stability. Furthermore, codon optimization and in silico cloning analysis were performed to check for efficient expression in a bacterial system. Collectively, these findings demonstrate the potential of the constructed vaccine to elicit an immune response against HHV-5 and HHV-6, thus supporting the viability of in vitro and in vivo studies.","PeriodicalId":12762,"journal":{"name":"Glycoconjugate Journal","volume":"39 6","pages":"711-724"},"PeriodicalIF":3.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9557995/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10349097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Glycoproteomic analysis reveals the effects of bisecting GlcNAc in intrahepatic cholangiocarcinoma. 糖蛋白组学分析揭示了分割GlcNAc在肝内胆管癌中的作用。

IF 3 4区生物学

Glycoconjugate Journal Pub Date : 2022-12-01 DOI: 10.1007/s10719-022-10085-5

Wei Dan, Cheng Li, Jun Li, Pengfei Li, Miaomiao Xin, Zexuan Chen, Liuyi Dang, Zihao Yu, Jing Li, Jiechen Shen, Liangshuo Hu, Shisheng Sun

{"title":"Glycoproteomic analysis reveals the effects of bisecting GlcNAc in intrahepatic cholangiocarcinoma.","authors":"Wei Dan, Cheng Li, Jun Li, Pengfei Li, Miaomiao Xin, Zexuan Chen, Liuyi Dang, Zihao Yu, Jing Li, Jiechen Shen, Liangshuo Hu, Shisheng Sun","doi":"10.1007/s10719-022-10085-5","DOIUrl":"https://doi.org/10.1007/s10719-022-10085-5","url":null,"abstract":"Intrahepatic cholangiocarcinoma (ICC) is the second major subtype of primary liver cancer and has caused more and more attention with increasing incidence and mortality worldwide. Our previous study found that bisecting N-glycans are commonly increased in ICC, while the effects and potential functions of bisecting GlcNAc in ICC are still largely unclear. In this study, we further confirmed that the structures of bisecting GlcNAc were significantly up-regulated in ICC compared with paracancer tissues by glycoproteomic data and lectin histochemistry. The expression of its glycosyltransferase MGAT3 was also up-regulated in ICC tissues at both mRNA and protein levels, and expression of MGAT3 is negatively correlated with overall survival explored by bioinformatic analyses and published datasets from 255 patients. Next, the silencing of MGAT3 could inhibit the growth and invasion of ICC cells, and overexpressing of MGAT3 only promoted ICC cell invasion. Further glycoproteomic analysis showed that the commonly glycoproteins modified by bisecting GlcNAc after MGAT3-overexpression in two ICC cell lines were mainly involved in cell movement-related biological processes, such as cell adhesion, integrin-related and ECM-receptor interaction. This study sheds light on the potential effects of bisecting GlcNAc in ICC cells and suggests that MGAT3 might be used as a potential target in the therapy of ICC.","PeriodicalId":12762,"journal":{"name":"Glycoconjugate Journal","volume":"39 6","pages":"737-745"},"PeriodicalIF":3.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10349626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of fermentation pH on the structure, rheological properties, and antioxidant activities of exopolysaccharides produced by Alteromonas australica QD. 发酵pH对澳大利亚异单胞菌胞外多糖结构、流变学特性及抗氧化活性的影响

IF 3 4区生物学

Glycoconjugate Journal Pub Date : 2022-12-01 DOI: 10.1007/s10719-022-10087-3

Fengshu Li, Xin Hu, Xiaojun Sun, Haoshuai Li, Jinren Lu, Yiming Li, Mutai Bao

{"title":"Effect of fermentation pH on the structure, rheological properties, and antioxidant activities of exopolysaccharides produced by Alteromonas australica QD.","authors":"Fengshu Li, Xin Hu, Xiaojun Sun, Haoshuai Li, Jinren Lu, Yiming Li, Mutai Bao","doi":"10.1007/s10719-022-10087-3","DOIUrl":"https://doi.org/10.1007/s10719-022-10087-3","url":null,"abstract":"The pH value was essential for the growth and metabolism of microorganisms. Acidic pH exopolysaccharide (AC-EPS) and alkaline pH exopolysaccharide (AL-EPS) secreted by A. australica QD mediated by pH were studied in this paper. The total carbohydrate content and molecular weight of AC-EPS (79.59% ± 2.24% (w/w), 8.374 × 105 Da) and AL-EPS (82.48% ± 1.46% (w/w), 6.182 × 105 Da) were estimated and compared. In AC-EPS, mannose (3.78%) and galactose (3.24%) content was more, while the proportion of glucuronic acid was less in comparison to AL-EPS. The scanning electron microscopy revealed the structural differences among the AC-EPS and AL-EPS. Thermogravimetric analysis showed degradation temperatures of 272.8 °C and 244.9 °C for AC-EPS and AL-EPS, respectively. AC-EPS was found to exhibit better rheological properties and emulsifying capabilities, while AL-EPS had superior antioxidant activities. Overall, both AC-EPS and AL-EPS have the potential to be used as emulsifiers and biological antioxidants.","PeriodicalId":12762,"journal":{"name":"Glycoconjugate Journal","volume":"39 6","pages":"773-787"},"PeriodicalIF":3.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10719413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

A water-soluble polysaccharide from Eucommia folium: the structural characterization and anti-tumor activity in vivo. 杜仲叶水溶性多糖的结构表征及体内抗肿瘤活性。

IF 3 4区生物学

Glycoconjugate Journal Pub Date : 2022-12-01 DOI: 10.1007/s10719-022-10086-4

Zhi-Qian Yan, Su-Yun Ding, Pei Chen, Hui-Ping Liu, Meng-Li Chang, Shu-Yuan Shi

{"title":"A water-soluble polysaccharide from Eucommia folium: the structural characterization and anti-tumor activity in vivo.","authors":"Zhi-Qian Yan, Su-Yun Ding, Pei Chen, Hui-Ping Liu, Meng-Li Chang, Shu-Yuan Shi","doi":"10.1007/s10719-022-10086-4","DOIUrl":"https://doi.org/10.1007/s10719-022-10086-4","url":null,"abstract":"In this study, a water-soluble polysaccharide from Eucommia folium was extracted by hot water and purified using Sephadex G-200 gel columns. The results showed that the purified fraction (EFP) has a molecular weight of 9.98 × 105 Da and consisted of rhamnose, arabinose, galactose, glucose, mannose, xylose, galacturonic acid, and glucuronic acid (molar ratio: 0.226: 1.739: 2.183: 1: 0.155: 0.321: 0.358: 0.047). The combination of infrared spectroscopy and NMR analysis proved that EFP is an acidic polysaccharide whose main chain consists of α-L-Araf-(1 → , → 3,5)-α-Araf-(1 → , → 3)-β-Galp-(1 → , → 3,6)-β-Glcp-(1 → , → 2)-α-D-Manp-(1 → , → 4)-α-GalpA-(1 → , → 2,4)-α-Rhap-(1 → . In addition, the in vivo antitumoral activity of EFP was studied using a H22 tumor-bearing mice model. EFP effectively inhibited tumor growth in mice following intragastric administration. By Combining with the results of the apoptosis assay and JC-1 staining analysis, we confirmed that EFP induces apoptosis through the mitochondrial pathway. Furthermore, cell cycle analysis demonstrated that EFP blocks the cell cycle at S phase.","PeriodicalId":12762,"journal":{"name":"Glycoconjugate Journal","volume":"39 6","pages":"759-772"},"PeriodicalIF":3.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10351406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Simple separation of glycosphingolipids in the lower phase of a Folch's partition from crude lipid fractions using zirconium dioxide. 使用二氧化锆从粗脂馏分中简单分离Folch分割下部相的鞘糖脂。

IF 3 4区生物学

Glycoconjugate Journal Pub Date : 2022-12-01 DOI: 10.1007/s10719-022-10080-w

Hideharu Nagasawa, Shota Miyazaki, Mamoru Kyogashima

{"title":"Simple separation of glycosphingolipids in the lower phase of a Folch's partition from crude lipid fractions using zirconium dioxide.","authors":"Hideharu Nagasawa, Shota Miyazaki, Mamoru Kyogashima","doi":"10.1007/s10719-022-10080-w","DOIUrl":"https://doi.org/10.1007/s10719-022-10080-w","url":null,"abstract":"A simple method was developed for the separation of glycosphingolipids (GSLs) from lipid mixtures, including phospholipids and cholesterol, using zirconium dioxide (zirconia, ZrO2). Although this procedure does not incorporate a mild alkali treatment, which is commonly used for eliminating glycerophospholipids, it can be used to remove both alkali-resistant sphingomyelin and glycerophospholipids possessing ether bonds. Importantly, when GSLs were dissolved in organic solvent together with cholesterol (Chol) and phospholipids, and loaded onto ZrO2, Chol did not bind to the ZrO2 but both the GSLs and phospholipids did. When eluted with 5 mg/mL of 2,5-dihydroxybenzoic acid in methanol, GSLs but not phospholipids were recovered, leaving the phospholipids bound to the ZrO2 particles. This method is particularly applicable for GSLs such as triglycosylceramides, tetraglycosylceramides and some pentaglycosylceramides, sulfatide and GM3 located in the lower phase of a Folch's partition, where significant amounts of phospholipids, Chol and neutral lipids reside along with GSLs. This method was successfully used to easily isolate GSLs from biological materials for their subsequent analysis by matrix-assisted laser desorption ionization time-of-flight mass spectrometry with high resolution.","PeriodicalId":12762,"journal":{"name":"Glycoconjugate Journal","volume":"39 6","pages":"789-795"},"PeriodicalIF":3.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10698951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

A homogalacturonan from Lonicera japonica Thunb. disrupts angiogenesis via epidermal growth factor receptor and Delta-like 4 associated signaling. 取自金银花(Lonicera japonica Thunb)的一种同型半乳糖酸。通过表皮生长因子受体和δ样4相关信号干扰血管生成。

IF 3 4区生物学

Glycoconjugate Journal Pub Date : 2022-12-01 DOI: 10.1007/s10719-022-10088-2

Wenfeng Liao, Xiaodong Hu, Zhenyun Du, Peipei Wang, Kan Ding

引用次数: 2