Glycoconjugate Journal最新文献_第9页

Analysis of the intramolecular 1,7-lactone of N-acetylneuraminic acid using HPLC-MS: relationship between detection and stability. n -乙酰神经氨酸分子内1,7-内酯的HPLC-MS分析:检测与稳定性的关系。

IF 3 4区生物学

Glycoconjugate Journal Pub Date : 2023-06-01 DOI: 10.1007/s10719-023-10114-x

Paolo La Rocca, Ivana Lavota, Marco Piccoli, Federica Cirillo, Andrea Ghiroldi, Giuseppe Ciconte, Carlo Pappone, Pietro Allevi, Paola Rota, Luigi Anastasia

{"title":"Analysis of the intramolecular 1,7-lactone of N-acetylneuraminic acid using HPLC-MS: relationship between detection and stability.","authors":"Paolo La Rocca, Ivana Lavota, Marco Piccoli, Federica Cirillo, Andrea Ghiroldi, Giuseppe Ciconte, Carlo Pappone, Pietro Allevi, Paola Rota, Luigi Anastasia","doi":"10.1007/s10719-023-10114-x","DOIUrl":"https://doi.org/10.1007/s10719-023-10114-x","url":null,"abstract":"A subclass of the sialic acid family consists of intramolecular lactones that may function as key indicators of physiological and pathological states. However, the existence of these compounds in free form is highly improbable, since they are unlikely to exist in an aqueous solution due to their lability. Current analytical method used to detect them in biological fluids has not recognized their reactivity in solution and is prone to misidentification. However, recent advances in synthetic methods for 1,7-lactones have allowed the preparation of these sialic acid derivatives as authentic reference standards. We report here the development of a new HPLC-MS method for the simultaneous detection of the 1,7-lactone of N-acetylneuraminic acid, its γ-lactone derivative, and N-acetylneuraminic acid that overcomes the limitations of the previous analytical procedure for their identification.","PeriodicalId":12762,"journal":{"name":"Glycoconjugate Journal","volume":"40 3","pages":"343-354"},"PeriodicalIF":3.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10202997/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9885323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sialylation of cell surface glycoconjugates modulates cytosolic galectin-mediated responses upon organelle damage : Minireview. 细胞表面糖缀合物的唾液化调节细胞器损伤时细胞内半乳糖凝集素介导的反应。

IF 3 4区生物学

Glycoconjugate Journal Pub Date : 2023-06-01 DOI: 10.1007/s10719-023-10112-z

I-Chun Weng, Hung-Lin Chen, Wei-Han Lin, Fu-Tong Liu

引用次数: 0

Synthesis and biological activity of ganglioside GM3 analogues with a (S)-CHF-Sialoside linkage and an alkyne tag. 具有(S)- chf -硅苷键和炔标记的神经节苷甘油酯GM3类似物的合成和生物活性。

IF 3 4区生物学

Glycoconjugate Journal Pub Date : 2023-06-01 DOI: 10.1007/s10719-023-10111-0

Eisuke Ota, Daiki Takeda, Kana Oonuma, Marie Kato, Hiroaki Matoba, Makoto Yoritate, Mikiko Sodeoka, Go Hirai

引用次数: 0

Recently developed glycosphingolipid probes and their dynamic behavior in cell plasma membranes as revealed by single-molecule imaging. 近年来研制的鞘糖脂探针及其在细胞膜中的动态行为的单分子成像研究。

IF 2.7 4区生物学

Glycoconjugate Journal Pub Date : 2023-06-01 Epub Date: 2023-05-03 DOI: 10.1007/s10719-023-10116-9

Kenichi G N Suzuki, Naoko Komura, Hiromune Ando

{"title":"Recently developed glycosphingolipid probes and their dynamic behavior in cell plasma membranes as revealed by single-molecule imaging.","authors":"Kenichi G N Suzuki, Naoko Komura, Hiromune Ando","doi":"10.1007/s10719-023-10116-9","DOIUrl":"10.1007/s10719-023-10116-9","url":null,"abstract":"Glycosphingolipids, including gangliosides, are representative lipid raft markers that perform a variety of physiological roles in cell membranes. However, studies aimed at revealing their dynamic behavior in living cells are rare, mostly due to a lack of suitable fluorescent probes. Recently, the ganglio-series, lacto-series, and globo-series glycosphingolipid probes, which mimic the behavior of the parental molecules in terms of partitioning to the raft fraction, were developed by conjugating hydrophilic dyes to the terminal glycans of glycosphingolipids using state-of-art entirely chemical-based synthetic techniques. High-speed, single-molecule observation of these fluorescent probes revealed that gangliosides were scarcely trapped in small domains (100 nm in diameter) for more than 5 ms in steady-state cells, suggesting that rafts including gangliosides were always moving and very small. Furthermore, dual-color, single-molecule observations clearly showed that homodimers and clusters of GPI-anchored proteins were stabilized by transiently recruiting sphingolipids, including gangliosides, to form homodimer rafts and the cluster rafts, respectively. In this review, we briefly summarize recent studies, the development of a variety of glycosphingolipid probes as well as the identification of the raft structures including gangliosides in living cells by single-molecule imaging.","PeriodicalId":12762,"journal":{"name":"Glycoconjugate Journal","volume":"40 3","pages":"305-314"},"PeriodicalIF":2.7,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9518978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Possible regulation of ganglioside GD3 synthase gene expression with DNA methylation in human glioma cells. 神经胶质瘤细胞DNA甲基化对神经节苷脂GD3合成酶基因表达的可能调控。

IF 3 4区生物学

Glycoconjugate Journal Pub Date : 2023-06-01 DOI: 10.1007/s10719-023-10108-9

Yurie Yamamoto, Ken Higashimoto, Yuki Ohkawa, Hidenobu Soejima, Kei Kaneko, Yuhsuke Ohmi, Keiko Furukawa, Koichi Furukawa

{"title":"Possible regulation of ganglioside GD3 synthase gene expression with DNA methylation in human glioma cells.","authors":"Yurie Yamamoto, Ken Higashimoto, Yuki Ohkawa, Hidenobu Soejima, Kei Kaneko, Yuhsuke Ohmi, Keiko Furukawa, Koichi Furukawa","doi":"10.1007/s10719-023-10108-9","DOIUrl":"https://doi.org/10.1007/s10719-023-10108-9","url":null,"abstract":"Gangliosides are expressed in nervous systems and some neuroectoderm-derived tumors at high levels and play pivotal roles. However, mechanisms for the regulation of glycosyltransferase genes responsible for the ganglioside synthesis are not well understood. In this study, we analyzed DNA methylation patterns of promoter regions of GD3 synthase (ST8SIA1) as well as mRNA levels and ganglioside expression using human glioma cell lines. Among 5 cell lines examined, 4 lines showed changes in the expression levels of related genes after treatment with 5-aza-dC. LN319 showed up-regulation of St8sia1 and increased b-series gangliosides after 5-aza-dC treatment, and an astrocytoma cell line, AS showed high expression of ST8SIA1 and b-series gangliosides persistently before and after 5-Aza-2'-deoxycytidine treatment. Using these 2 cell lines, DNA methylation patterns of the promoter regions of the gene were analyzed by bisulfite-sequencing. Consequently, 2 regions that were methylated before 5-Aza-2'-deoxycytidine treatment were demethylated in LN319 after the treatment, while those regions were persistently demethylated in AS. These 2 regions corresponded with sites defined as promoter regions by Luciferase assay. Taken together, it was suggested that ST8SIA1 gene is regulated by DNA methylation at the promoter regions, leading to the regulation of tumor phenotypes.","PeriodicalId":12762,"journal":{"name":"Glycoconjugate Journal","volume":"40 3","pages":"323-332"},"PeriodicalIF":3.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9515333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Serum total carbohydrates, conjugated carbohydrates and total protein glycation index in diabetes mellitus. 糖尿病患者血清总碳水化合物、共轭碳水化合物及总蛋白糖化指数。

IF 3 4区生物学

Glycoconjugate Journal Pub Date : 2023-06-01 DOI: 10.1007/s10719-023-10115-w

Sepideh Hosseini, Saeid Abediankenari, Mehdi Rasouli

{"title":"Serum total carbohydrates, conjugated carbohydrates and total protein glycation index in diabetes mellitus.","authors":"Sepideh Hosseini, Saeid Abediankenari, Mehdi Rasouli","doi":"10.1007/s10719-023-10115-w","DOIUrl":"https://doi.org/10.1007/s10719-023-10115-w","url":null,"abstract":"Background: Diabetes mellitus is defined according to fasting blood glucose and clinical signs. But, the markers of glycation have been used recently as a criterion to diagnose and monitor the therapy.Objectives: To measure serum total- and conjugated- saccharides and to define the new marker as serum total protein glycation index (sTPGI ) for diabetes.Design and methods: The study population consisted of 172 subjects who were divided to control and diabetic cases. Serum total and conjugated saccharides were measured and sTPGI was defined to discriminate serum glycosylated and glycated saccharides.Results: Patients with diabetes compared with the controls had increased levels of serum (free) glucose, HbA1c, serum total carbohydrates, total conjugated carbohydrates and sTPGI. All three indices of serum carbohydrates showed significant positive correlation with serum glucose, HbA1c and diabetes. The equations: sTPGI = 0.12 Glucose (mg/dL) + 12 and sTPGI = 3.5HbA1c (%) + 5, were deduced for the association of sTPGI with serum free glucose and HbA1c. In ROC analysis, both HbA1c (AUC = 0.965, p ≤ 0.001) and sTPGI (AUC = 0.734, p ≤ 0.001) had strong and significant efficiency to discriminate diabetic cases from control subjects.Conclusions: The results confirm that sTPGI obtained by indirect assay has high significant efficiency comparable to HbA1c to diagnose diabetes. sTPGI relative to HbA1c indicates the mean level of glycaemia over a shorter period of about one month so it responds more quickly to changes in therapy.","PeriodicalId":12762,"journal":{"name":"Glycoconjugate Journal","volume":"40 3","pages":"375-381"},"PeriodicalIF":3.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9885302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Alkali-labile gangliosides. Alkali-labile神经节甘脂。

IF 3 4区生物学

Glycoconjugate Journal Pub Date : 2023-06-01 DOI: 10.1007/s10719-023-10103-0

Laura Mauri, Sandro Sonnino

引用次数: 0

Advances in protein glycosylation and its role in tissue repair and regeneration. 蛋白糖基化及其在组织修复和再生中的作用研究进展。

IF 3 4区生物学

Glycoconjugate Journal Pub Date : 2023-06-01 DOI: 10.1007/s10719-023-10117-8

Zhongyu Yue, Yajie Yu, Boyuan Gao, Du Wang, Hongxiao Sun, Yue Feng, Zihan Ma, Xin Xie

{"title":"Advances in protein glycosylation and its role in tissue repair and regeneration.","authors":"Zhongyu Yue, Yajie Yu, Boyuan Gao, Du Wang, Hongxiao Sun, Yue Feng, Zihan Ma, Xin Xie","doi":"10.1007/s10719-023-10117-8","DOIUrl":"https://doi.org/10.1007/s10719-023-10117-8","url":null,"abstract":"After tissue damage, a series of molecular and cellular events are initiated to promote tissue repair and regeneration to restore its original structure and function. These events include inter-cell communication, cell proliferation, cell migration, extracellular matrix differentiation, and other critical biological processes. Glycosylation is the crucial conservative and universal post-translational modification in all eukaryotic cells [1], with influential roles in intercellular recognition, regulation, signaling, immune response, cellular transformation, and disease development. Studies have shown that abnormally glycosylation of proteins is a well-recognized feature of cancer cells, and specific glycan structures are considered markers of tumor development. There are many studies on gene expression and regulation during tissue repair and regeneration. Still, there needs to be more knowledge of complex carbohydrates' effects on tissue repair and regeneration, such as glycosylation. Here, we present a review of studies investigating protein glycosylation in the tissue repair and regeneration process.","PeriodicalId":12762,"journal":{"name":"Glycoconjugate Journal","volume":"40 3","pages":"355-373"},"PeriodicalIF":3.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9885328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Improved synthesis of CD22-binding sialosides and its application for further development of potent CD22 inhibitors. CD22结合涎苷的改进合成及其在进一步开发有效CD22抑制剂中的应用。

IF 3 4区生物学

Glycoconjugate Journal Pub Date : 2023-04-01 DOI: 10.1007/s10719-023-10098-8

Yuki Suganuma, Akihiro Imamura, Hiromune Ando, Makoto Kiso, Hiromu Takematsu, Takeshi Tsubata, Hideharu Ishida

{"title":"Improved synthesis of CD22-binding sialosides and its application for further development of potent CD22 inhibitors.","authors":"Yuki Suganuma, Akihiro Imamura, Hiromune Ando, Makoto Kiso, Hiromu Takematsu, Takeshi Tsubata, Hideharu Ishida","doi":"10.1007/s10719-023-10098-8","DOIUrl":"https://doi.org/10.1007/s10719-023-10098-8","url":null,"abstract":"CD22, one of the sialic acid-binding immunoglobulin-like lectins (Siglecs), regulates B lymphocyte signaling via its interaction with glycan ligands bearing the sequence Neu5Ac/Gcα(2→6)Gal. We have developed the synthetic sialoside GSC-718 as a ligand mimic for CD22 and identified it as a potent CD22 inhibitor. Although the synthesis of CD22-binding sialosides including GSC-718 has been reported by our group, the synthetic route was unfortunately not suitable for large-scale synthesis. In this study, we developed an improved scalable synthetic procedure for sialosides which utilized 1,5-lactam formation as a key step. The improved procedure yielded sialosides incorporating a series of aglycones at the C2 position. Several derivatives with substituted benzyl residues as aglycones were found to bind to mouse CD22 with affinity comparable to that of GSC-718. The new procedure developed in this study affords sialosides in sufficient quantities for cell-based assays, and will facilitate the search for promising CD22 inhibitors that have therapeutic potential.","PeriodicalId":12762,"journal":{"name":"Glycoconjugate Journal","volume":"40 2","pages":"225-246"},"PeriodicalIF":3.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9648544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Twin research shows glycan changes are more susceptible to environmental factors than their carrier glycoproteins. 双胞胎研究表明，聚糖的变化比其载体糖蛋白更容易受到环境因素的影响。

IF 3 4区生物学

Glycoconjugate Journal Pub Date : 2023-04-01 DOI: 10.1007/s10719-023-10099-7

Tatsuya Asuka, Yoshihiro Kamada, Koichi Morishita, Tomoya Fukuoka, Shinji Takamatsu, Jumpei Kondo, Mikio Watanabe, Norio Sakai, Kazuo Hayakawa, Eiji Miyoshi

{"title":"Twin research shows glycan changes are more susceptible to environmental factors than their carrier glycoproteins.","authors":"Tatsuya Asuka, Yoshihiro Kamada, Koichi Morishita, Tomoya Fukuoka, Shinji Takamatsu, Jumpei Kondo, Mikio Watanabe, Norio Sakai, Kazuo Hayakawa, Eiji Miyoshi","doi":"10.1007/s10719-023-10099-7","DOIUrl":"https://doi.org/10.1007/s10719-023-10099-7","url":null,"abstract":"Changes in protein glycosylation are clinically used as biomarkers. In the present study, we employed a twin cohort to investigate the contributions of genetic and environmental factors to glycan modifications of glycoproteins. Mac-2 binding protein (Mac-2 bp), haptoglobin (Hp), and their glycosylated forms are liver fibrosis and cancer biomarkers. Sera from 107 twin pairs without clinical information were used as a training cohort for the Mac-2 bp and Mac-2 bp glycosylation isomer (M2BPGi) assay. As a validation cohort, 22 twin pairs were enrolled in the study. For each twin pair, one twin was diagnosed with liver or pancreatic disease. For the training cohort, the correlation ratios of serum Mac-2 bp and M2BPGi levels in twin sera with random sequences were 0.30 and 0.018, respectively. The correlation ratios between twin pairs in the validation cohort for serum Mac-2 bp and M2BPGi levels were 0.75 and 0.35, respectively. In contrast, correlation ratios of serum Hp and fucosylated haptoglobin (Fuc-Hp) levels between twin sera with liver and pancreatic disease were 0.49 and 0.16, respectively. Although serum protein levels of glycoproteins are susceptible to genetic factors, characteristic glycan changes of these glycoproteins are more susceptible to environmental factors, including liver and pancreatic disease.","PeriodicalId":12762,"journal":{"name":"Glycoconjugate Journal","volume":"40 2","pages":"191-198"},"PeriodicalIF":3.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9649063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0