Glycoconjugate Journal最新文献_第6页

Recognition factors of Dolichos biflorus agglutinin (DBA) and their accommodation sites. 芍药凝集素(DBA)的识别因子及其调节位点。

IF 3 4区生物学

Glycoconjugate Journal Pub Date : 2023-08-01 DOI: 10.1007/s10719-023-10118-7

Albert M Wu, Anna Dudek, Yung Liang Chen

{"title":"Recognition factors of Dolichos biflorus agglutinin (DBA) and their accommodation sites.","authors":"Albert M Wu, Anna Dudek, Yung Liang Chen","doi":"10.1007/s10719-023-10118-7","DOIUrl":"https://doi.org/10.1007/s10719-023-10118-7","url":null,"abstract":"Dolichos biflorus agglutinin (DBA) is one of the well known plant lectins that are widely used in clinical serology to differentiate human blood group A1 and A2 erythrocytes and also applied to glycobiology. However, the knowledge of recognition factors of polyvalent (super) glycotopes in glycans and the roles of functional group and epimer in monosaccharide (sub-monosaccharide recognition factor) have not been well established. The size and shape of the recognition (combining) site of DBA has not been clearly defined. In this study, many importnat recognition factors of DBA-glycan binding were characterized by our established enzyme-linked lectinosorbent (ELLSA) and inhibition assays. The results of these assays showed that the intensity profile of the recognition factors for the major combining site of DBA was expressed by Mass relative potency (Mass R.P.) and shown by decreasing order of high density of polyvalent GalNAcα1 → (super glycotopes, 3.7 × 103) >> the corresponding β anomers >> monomeric GalNAcα1 → related glycotopes (GalNAc as 1.0) >> their GalNAc β-anomers >> Gal (absence of NHCH3CO at carbon-2 of GAlNAc) and GlcNAc (different epimer of Carbon-4 in GalNAc). From the all data available, it is proposed that the combining site of DBA should consist of a small cavity shape as major site and most complementary to monomeric GalNAcα → located at both terminal reducing end (Tn) and nonreducing end of glycan chains, and with a wide and broad area as subsite to accomodate from mono- to tetra-saccharides (GalNAcβ, Galβ1 → 3/4GlcNAc, lFuc1 → 2Galβ1 → 3/4GlcNAc, GalNAcβ1 → 3Galα1 → 4Galβ1 → 4Glc) at the nonreducing side. In this study, it has provided the most (comprehensive) recognition knowledge of DBA-glycan interactions at the factors of glycotope, super glycotope/sub-monosaccharide levels. Thus, it should expand and upgrade the conventional concept of the combining (recognition) site of DBA since 1980s.","PeriodicalId":12762,"journal":{"name":"Glycoconjugate Journal","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10239814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Neuroimmunomodulatory properties of polysialic acid. 聚唾液酸的神经免疫调节特性。

IF 3 4区生物学

Glycoconjugate Journal Pub Date : 2023-06-01 DOI: 10.1007/s10719-023-10120-z

Lina Gretenkort, Hauke Thiesler, Herbert Hildebrandt

引用次数: 0

Vibrio-binding gangliosides in fish intestinal tracts. 鱼类肠道中与弧菌结合的神经节苷。

IF 3 4区生物学

Glycoconjugate Journal Pub Date : 2023-06-01 DOI: 10.1007/s10719-023-10110-1

Makoto Ito, Shinichi Chisada, Naoyuki Matsunaga, Nozomu Okino

引用次数: 0

Analysis of the intramolecular 1,7-lactone of N-acetylneuraminic acid using HPLC-MS: relationship between detection and stability. n -乙酰神经氨酸分子内1,7-内酯的HPLC-MS分析:检测与稳定性的关系。

IF 3 4区生物学

Glycoconjugate Journal Pub Date : 2023-06-01 DOI: 10.1007/s10719-023-10114-x

Paolo La Rocca, Ivana Lavota, Marco Piccoli, Federica Cirillo, Andrea Ghiroldi, Giuseppe Ciconte, Carlo Pappone, Pietro Allevi, Paola Rota, Luigi Anastasia

{"title":"Analysis of the intramolecular 1,7-lactone of N-acetylneuraminic acid using HPLC-MS: relationship between detection and stability.","authors":"Paolo La Rocca, Ivana Lavota, Marco Piccoli, Federica Cirillo, Andrea Ghiroldi, Giuseppe Ciconte, Carlo Pappone, Pietro Allevi, Paola Rota, Luigi Anastasia","doi":"10.1007/s10719-023-10114-x","DOIUrl":"https://doi.org/10.1007/s10719-023-10114-x","url":null,"abstract":"A subclass of the sialic acid family consists of intramolecular lactones that may function as key indicators of physiological and pathological states. However, the existence of these compounds in free form is highly improbable, since they are unlikely to exist in an aqueous solution due to their lability. Current analytical method used to detect them in biological fluids has not recognized their reactivity in solution and is prone to misidentification. However, recent advances in synthetic methods for 1,7-lactones have allowed the preparation of these sialic acid derivatives as authentic reference standards. We report here the development of a new HPLC-MS method for the simultaneous detection of the 1,7-lactone of N-acetylneuraminic acid, its γ-lactone derivative, and N-acetylneuraminic acid that overcomes the limitations of the previous analytical procedure for their identification.","PeriodicalId":12762,"journal":{"name":"Glycoconjugate Journal","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10202997/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9885323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sialylation of cell surface glycoconjugates modulates cytosolic galectin-mediated responses upon organelle damage : Minireview. 细胞表面糖缀合物的唾液化调节细胞器损伤时细胞内半乳糖凝集素介导的反应。

IF 3 4区生物学

Glycoconjugate Journal Pub Date : 2023-06-01 DOI: 10.1007/s10719-023-10112-z

I-Chun Weng, Hung-Lin Chen, Wei-Han Lin, Fu-Tong Liu

引用次数: 0

Synthesis and biological activity of ganglioside GM3 analogues with a (S)-CHF-Sialoside linkage and an alkyne tag. 具有(S)- chf -硅苷键和炔标记的神经节苷甘油酯GM3类似物的合成和生物活性。

IF 3 4区生物学

Glycoconjugate Journal Pub Date : 2023-06-01 DOI: 10.1007/s10719-023-10111-0

Eisuke Ota, Daiki Takeda, Kana Oonuma, Marie Kato, Hiroaki Matoba, Makoto Yoritate, Mikiko Sodeoka, Go Hirai

引用次数: 0

Recently developed glycosphingolipid probes and their dynamic behavior in cell plasma membranes as revealed by single-molecule imaging. 近年来研制的鞘糖脂探针及其在细胞膜中的动态行为的单分子成像研究。

IF 3 4区生物学

Glycoconjugate Journal Pub Date : 2023-06-01 DOI: 10.1007/s10719-023-10116-9

Kenichi G N Suzuki, Naoko Komura, Hiromune Ando

{"title":"Recently developed glycosphingolipid probes and their dynamic behavior in cell plasma membranes as revealed by single-molecule imaging.","authors":"Kenichi G N Suzuki, Naoko Komura, Hiromune Ando","doi":"10.1007/s10719-023-10116-9","DOIUrl":"https://doi.org/10.1007/s10719-023-10116-9","url":null,"abstract":"Glycosphingolipids, including gangliosides, are representative lipid raft markers that perform a variety of physiological roles in cell membranes. However, studies aimed at revealing their dynamic behavior in living cells are rare, mostly due to a lack of suitable fluorescent probes. Recently, the ganglio-series, lacto-series, and globo-series glycosphingolipid probes, which mimic the behavior of the parental molecules in terms of partitioning to the raft fraction, were developed by conjugating hydrophilic dyes to the terminal glycans of glycosphingolipids using state-of-art entirely chemical-based synthetic techniques. High-speed, single-molecule observation of these fluorescent probes revealed that gangliosides were scarcely trapped in small domains (100 nm in diameter) for more than 5 ms in steady-state cells, suggesting that rafts including gangliosides were always moving and very small. Furthermore, dual-color, single-molecule observations clearly showed that homodimers and clusters of GPI-anchored proteins were stabilized by transiently recruiting sphingolipids, including gangliosides, to form homodimer rafts and the cluster rafts, respectively. In this review, we briefly summarize recent studies, the development of a variety of glycosphingolipid probes as well as the identification of the raft structures including gangliosides in living cells by single-molecule imaging.","PeriodicalId":12762,"journal":{"name":"Glycoconjugate Journal","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9518978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Possible regulation of ganglioside GD3 synthase gene expression with DNA methylation in human glioma cells. 神经胶质瘤细胞DNA甲基化对神经节苷脂GD3合成酶基因表达的可能调控。

IF 3 4区生物学

Glycoconjugate Journal Pub Date : 2023-06-01 DOI: 10.1007/s10719-023-10108-9

Yurie Yamamoto, Ken Higashimoto, Yuki Ohkawa, Hidenobu Soejima, Kei Kaneko, Yuhsuke Ohmi, Keiko Furukawa, Koichi Furukawa

{"title":"Possible regulation of ganglioside GD3 synthase gene expression with DNA methylation in human glioma cells.","authors":"Yurie Yamamoto, Ken Higashimoto, Yuki Ohkawa, Hidenobu Soejima, Kei Kaneko, Yuhsuke Ohmi, Keiko Furukawa, Koichi Furukawa","doi":"10.1007/s10719-023-10108-9","DOIUrl":"https://doi.org/10.1007/s10719-023-10108-9","url":null,"abstract":"Gangliosides are expressed in nervous systems and some neuroectoderm-derived tumors at high levels and play pivotal roles. However, mechanisms for the regulation of glycosyltransferase genes responsible for the ganglioside synthesis are not well understood. In this study, we analyzed DNA methylation patterns of promoter regions of GD3 synthase (ST8SIA1) as well as mRNA levels and ganglioside expression using human glioma cell lines. Among 5 cell lines examined, 4 lines showed changes in the expression levels of related genes after treatment with 5-aza-dC. LN319 showed up-regulation of St8sia1 and increased b-series gangliosides after 5-aza-dC treatment, and an astrocytoma cell line, AS showed high expression of ST8SIA1 and b-series gangliosides persistently before and after 5-Aza-2'-deoxycytidine treatment. Using these 2 cell lines, DNA methylation patterns of the promoter regions of the gene were analyzed by bisulfite-sequencing. Consequently, 2 regions that were methylated before 5-Aza-2'-deoxycytidine treatment were demethylated in LN319 after the treatment, while those regions were persistently demethylated in AS. These 2 regions corresponded with sites defined as promoter regions by Luciferase assay. Taken together, it was suggested that ST8SIA1 gene is regulated by DNA methylation at the promoter regions, leading to the regulation of tumor phenotypes.","PeriodicalId":12762,"journal":{"name":"Glycoconjugate Journal","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9515333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Alkali-labile gangliosides. Alkali-labile神经节甘脂。

IF 3 4区生物学

Glycoconjugate Journal Pub Date : 2023-06-01 DOI: 10.1007/s10719-023-10103-0

Laura Mauri, Sandro Sonnino

引用次数: 0

Serum total carbohydrates, conjugated carbohydrates and total protein glycation index in diabetes mellitus. 糖尿病患者血清总碳水化合物、共轭碳水化合物及总蛋白糖化指数。

IF 3 4区生物学

Glycoconjugate Journal Pub Date : 2023-06-01 DOI: 10.1007/s10719-023-10115-w

Sepideh Hosseini, Saeid Abediankenari, Mehdi Rasouli

{"title":"Serum total carbohydrates, conjugated carbohydrates and total protein glycation index in diabetes mellitus.","authors":"Sepideh Hosseini, Saeid Abediankenari, Mehdi Rasouli","doi":"10.1007/s10719-023-10115-w","DOIUrl":"https://doi.org/10.1007/s10719-023-10115-w","url":null,"abstract":"Background: Diabetes mellitus is defined according to fasting blood glucose and clinical signs. But, the markers of glycation have been used recently as a criterion to diagnose and monitor the therapy.Objectives: To measure serum total- and conjugated- saccharides and to define the new marker as serum total protein glycation index (sTPGI ) for diabetes.Design and methods: The study population consisted of 172 subjects who were divided to control and diabetic cases. Serum total and conjugated saccharides were measured and sTPGI was defined to discriminate serum glycosylated and glycated saccharides.Results: Patients with diabetes compared with the controls had increased levels of serum (free) glucose, HbA1c, serum total carbohydrates, total conjugated carbohydrates and sTPGI. All three indices of serum carbohydrates showed significant positive correlation with serum glucose, HbA1c and diabetes. The equations: sTPGI = 0.12 Glucose (mg/dL) + 12 and sTPGI = 3.5HbA1c (%) + 5, were deduced for the association of sTPGI with serum free glucose and HbA1c. In ROC analysis, both HbA1c (AUC = 0.965, p ≤ 0.001) and sTPGI (AUC = 0.734, p ≤ 0.001) had strong and significant efficiency to discriminate diabetic cases from control subjects.Conclusions: The results confirm that sTPGI obtained by indirect assay has high significant efficiency comparable to HbA1c to diagnose diabetes. sTPGI relative to HbA1c indicates the mean level of glycaemia over a shorter period of about one month so it responds more quickly to changes in therapy.","PeriodicalId":12762,"journal":{"name":"Glycoconjugate Journal","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9885302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0