Glycoconjugate Journal最新文献_第5页

A concise chemoenzymatic total synthesis of neutral Globo-series glycosphingolipids Globo A and Globo B, and Forssman and para-Forssman antigens. 中性Globo系列鞘糖脂Globo A和Globo B以及Forssman和para-Forssman抗原的简明化学酶全合成。

IF 3 4区生物学

Glycoconjugate Journal Pub Date : 2023-10-01 Epub Date: 2023-08-22 DOI: 10.1007/s10719-023-10133-8

Yu-Ching Chiang, Chun-Yen Wu, Pei-Yun Chiang, Avijit K Adak, Chun-Cheng Lin

{"title":"A concise chemoenzymatic total synthesis of neutral Globo-series glycosphingolipids Globo A and Globo B, and Forssman and para-Forssman antigens.","authors":"Yu-Ching Chiang, Chun-Yen Wu, Pei-Yun Chiang, Avijit K Adak, Chun-Cheng Lin","doi":"10.1007/s10719-023-10133-8","DOIUrl":"10.1007/s10719-023-10133-8","url":null,"abstract":"Globo A is a neutral Globo-series glycosphingolipid (GSL) that shows natural properties of a cytotoxicity receptor NKp44 binding ligand. The highly complex heptasaccharide glycan structure of Globo A combined with its biological profile provides a unique target for the development of a synthetic method to facilitate its bioactivity studies. Here, a concise chemoenzymatic route to the synthesis of Globo A and its α1,3-galactose-linked congener Globo B is reported. The key to success was the use of a synthetic azido β-Globo H sphingosine (Globo H-βSph) as an acceptor substrate and two glycosyl transferases, an α1,3-N-acetylgalactosaminyltransferase from Helicobacter mustelae (BgtA) and a human blood group B α1,3-galactosyltransferase (h1,3GTB), for stereoselective construction of the terminal α1,3-GalNAc and α1,3-Gal linkages, respectively. The azido-Sph lipid sidechain is further elaborated by reduction and a chemoselective N-acylation to complete the total synthesis of the neutral Globo-series GSLs. In addition, the synthesis of Forssman and para-Forssman antigens were prepared. The strategy may be suitable for accessing other complex GSLs and related lipid-modified GSL derivatives.","PeriodicalId":12762,"journal":{"name":"Glycoconjugate Journal","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10042620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Roles of the structural units, glycotopes / mammalian N-glycans for Con A-glycan interactions, their codes, and their recognition factors. 结构单元、糖基/哺乳动物n -聚糖在Con - a -聚糖相互作用中的作用、它们的编码和它们的识别因子。

IF 3 4区生物学

Glycoconjugate Journal Pub Date : 2023-10-01 Epub Date: 2023-09-11 DOI: 10.1007/s10719-023-10129-4

Albert M Wu

{"title":"Roles of the structural units, glycotopes / mammalian N-glycans for Con A-glycan interactions, their codes, and their recognition factors.","authors":"Albert M Wu","doi":"10.1007/s10719-023-10129-4","DOIUrl":"10.1007/s10719-023-10129-4","url":null,"abstract":"The binding property of Con A has been studied intensively and applied widely to glycoconjugates / glycobiology for over 80 years. However, its role and functional relationship of Con A with these mammalian structural units, glycotopes, N-glycan chains, as well as their polyvalent forms in N-glycoproteins involved in the Con A-glycan interactions have not been well defined and organized. In this study, the recognition factors involved in these interactions were analyzed by our well developed method- the enzyme linked lectinosorbent (ELLSA) and inhibition assay. Based on all the data obtained, it is concluded that Con A, as previously reported, has a relatively broad and wide recognition ability of the Manα1 → and Glcα1 → related glycans. It reacted not only strongly with yeast mannan and glycogens, but also bound well with a large number of mammalian N-glycans, including the N-glycans of rat sublingual gp (RSL), human Tamm-Horsfall glycoprotein (THGP), thyroglobulin and lactoferrin. The recognition specificity of Con A towards ligands, expressed by Molar Relative Potency (Molar R.P.), in a decreasing order is as follows: α1 → 3, α1 → 6 Mannopentaose (M5) and Biantennary N-linked core pentasaccharide (MDi) ≥ α1 → 3, α1 → 6 Mannotriose (M3) > Manα1 → 3Man (α1 → 3Mannobiose), Manα1 → 2Man (α1 → 2Mannobiose), Manα1 → 6Man (α1 → 6Mannobiose), Manα1 → 4Man (α1 → 4Mannobiose) > GlcNAcβ1 → 2Man (β1 → 2 N-Acetyl glucosamine-mannose) > Manα1 → /Glcα1 → > Man > Glc, while Gal / GalNAc were inactive. Furthermore, the Man related code system, in this study, is proposed to express by both numbers of Man and GlcNAcβ1 → branches (M3 to M9 / MMono to Penta etc.) and a table of three Manα1 → and Glcα1 → related biomasses of six recognition factors involved in the Con A-glycan interactions has also been demonstrated. These themes should be one of the most valuable advances since 1980s.","PeriodicalId":12762,"journal":{"name":"Glycoconjugate Journal","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10194443","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Straightforward synthesis of the hexasaccharide repeating unit of the O-specific polysaccharide of Salmonella arizonae O62. 直接合成亚利桑那沙门氏菌O62的o特异性多糖的六糖重复单元。

IF 3 4区生物学

Glycoconjugate Journal Pub Date : 2023-08-01 DOI: 10.1007/s10719-023-10122-x

Abhijit Rana, Pradip Shit, Anup Kumar Misra

引用次数: 0

Unravelling the genetic causality of immunoglobulin G N-glycans in ischemic stroke. 揭示免疫球蛋白G - n -聚糖在缺血性卒中中的遗传因果关系。

IF 3 4区生物学

Glycoconjugate Journal Pub Date : 2023-08-01 DOI: 10.1007/s10719-023-10127-6

Biyan Wang, Lei Gao, Jie Zhang, Xiaoni Meng, Xizhu Xu, Haifeng Hou, Weijia Xing, Wei Wang, Youxin Wang

{"title":"Unravelling the genetic causality of immunoglobulin G N-glycans in ischemic stroke.","authors":"Biyan Wang, Lei Gao, Jie Zhang, Xiaoni Meng, Xizhu Xu, Haifeng Hou, Weijia Xing, Wei Wang, Youxin Wang","doi":"10.1007/s10719-023-10127-6","DOIUrl":"https://doi.org/10.1007/s10719-023-10127-6","url":null,"abstract":"Background: Evidence suggests that immunoglobulin G (IgG) N-glycosylation is associated with ischemic stroke (IS). However, the causality of IgG N-glycosylation for IS remains unknown.Methods: Two-sample Mendelian randomization (MR) analyses were performed to investigate the potential causal effects of genetically determined IgG N-glycans on IS using publicly available summarized genetic data from East Asian and European populations. Genetic instruments were used as proxies for IgG N-glycan traits. IgG N-glycans were analysed using ultra-performance liquid chromatography. Four complementary MR methods were performed, including the inverse variance weighted method (IVW), MR‒Egger, weighted median and penalized weighted median. Furthermore, to further test the robustness of the results, MR based on Bayesian model averaging (MR-BMA) was then applied to select and prioritize IgG N-glycan traits as risk factors for IS.Results: After correcting for multiple testing, in two-sample MR analyses, genetically predicted IgG N-glycans were unrelated to IS in both East Asian and European populations, and the results remained consistent and robust in the sensitivity analysis. Moreover, MR-BMA also showed consistent results in both East Asian and European populations.Conclusions: Contrary to observational studies, the study did not provide enough genetic evidence to support the causal associations of genetically predicted IgG N-glycan traits and IS, suggesting that N-glycosylation of IgG might not directly involve in the pathogenesis of IS.","PeriodicalId":12762,"journal":{"name":"Glycoconjugate Journal","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10290961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mucin glycans and their degradation by gut microbiota. 粘蛋白聚糖及其在肠道微生物群中的降解。

IF 3 4区生物学

Glycoconjugate Journal Pub Date : 2023-08-01 DOI: 10.1007/s10719-023-10124-9

Masanori Yamaguchi, Kenji Yamamoto

{"title":"Mucin glycans and their degradation by gut microbiota.","authors":"Masanori Yamaguchi, Kenji Yamamoto","doi":"10.1007/s10719-023-10124-9","DOIUrl":"https://doi.org/10.1007/s10719-023-10124-9","url":null,"abstract":"The human intestinal tract is inhabited by a tremendous number of microorganisms, which are collectively termed \"the gut microbiota\". The intestinal epithelium is covered with a dense layer of mucus that prevents penetration of the gut microbiota into underlying tissues of the host. Recent studies have shown that the maturation and function of the mucus layer are strongly influenced by the gut microbiota, and alteration in the structure and function of the gut microbiota is implicated in several diseases. Because the intestinal mucus layer is at a crucial interface between microbes and their host, its breakdown leads to gut bacterial invasion that can eventually cause inflammation and infection. The mucus is composed of mucin, which is rich in glycans, and the various structures of the complex carbohydrates of mucins can select for distinct mucosa-associated bacteria that are able to bind mucin glycans, and sometimes degrade them as a nutrient source. Mucin glycans are diverse molecules, and thus mucin glycan degradation is a complex process that requires a broad range of glycan-degrading enzymes. Because of the increased recognition of the role of mucus-associated microbes in human health, how commensal bacteria degrade and use host mucin glycans has become of increased interest. This review provides an overview of the relationships between the mucin glycan of the host and gut commensal bacteria, with a focus on mucin degradation.","PeriodicalId":12762,"journal":{"name":"Glycoconjugate Journal","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10234835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

The vertebrate sialylation machinery: structure-function and molecular evolution of GT-29 sialyltransferases. 脊椎动物唾液基化机制:GT-29唾液基转移酶的结构、功能和分子进化。

IF 3 4区生物学

Glycoconjugate Journal Pub Date : 2023-08-01 DOI: 10.1007/s10719-023-10123-w

Anne Harduin-Lepers

{"title":"The vertebrate sialylation machinery: structure-function and molecular evolution of GT-29 sialyltransferases.","authors":"Anne Harduin-Lepers","doi":"10.1007/s10719-023-10123-w","DOIUrl":"https://doi.org/10.1007/s10719-023-10123-w","url":null,"abstract":"Every eukaryotic cell is covered with a thick layer of complex carbohydrates with essential roles in their social life. In Deuterostoma, sialic acids present at the outermost positions of glycans of glycoconjugates are known to be key players in cellular interactions including host-pathogen interactions. Their negative charge and hydrophilic properties enable their roles in various normal and pathological states and their expression is altered in many diseases including cancers. Sialylation of glycoproteins and glycolipids is orchestrated by the regulated expression of twenty sialyltransferases in human tissues with distinct enzymatic characteristics and preferences for substrates and linkages formed. However, still very little is known on the functional organization of sialyltransferases in the Golgi apparatus and how the sialylation machinery is finely regulated to provide the ad hoc sialome to the cell. This review summarizes current knowledge on sialyltransferases, their structure-function relationships, molecular evolution, and their implications in human biology.","PeriodicalId":12762,"journal":{"name":"Glycoconjugate Journal","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10225777/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10227744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Interactions between polysialic acid and dopamine-lead compounds as revealed by biochemical and in silico docking simulation analyses. 聚唾液酸和多巴胺铅化合物之间的相互作用揭示了生化和硅对接模拟分析。

IF 3 4区生物学

Glycoconjugate Journal Pub Date : 2023-08-01 DOI: 10.1007/s10719-023-10119-6

Kaito Hayakawa, Masaya Hane, Hiroki Hamagami, Miki Imai, Hiroshi Tanaka, Ken Kitajima, Chihiro Sato

引用次数: 0

ST3GalIV drives SLeX biosynthesis in gastrointestinal cancer cells and associates with cancer cell motility. ST3GalIV驱动胃肠道癌细胞的SLeX生物合成并与癌细胞运动相关。

IF 3 4区生物学

Glycoconjugate Journal Pub Date : 2023-08-01 DOI: 10.1007/s10719-023-10113-y

Ana F Costa, Emanuel Senra, Isabel Faria-Ramos, Andreia Teixeira, João Morais, Mariana Pacheco, Celso A Reis, Catarina Gomes

{"title":"ST3GalIV drives SLeX biosynthesis in gastrointestinal cancer cells and associates with cancer cell motility.","authors":"Ana F Costa, Emanuel Senra, Isabel Faria-Ramos, Andreia Teixeira, João Morais, Mariana Pacheco, Celso A Reis, Catarina Gomes","doi":"10.1007/s10719-023-10113-y","DOIUrl":"https://doi.org/10.1007/s10719-023-10113-y","url":null,"abstract":"Expression of sialyl Lewis X (SLeX) is a well-documented event during malignant transformation of cancer cells, and largely associates with their invasive and metastatic properties. Glycoproteins and glycolipids are the main carriers of SLeX, whose biosynthesis is known to be performed by different glycosyltransferases, namely by the family of β-galactoside-α2,3-sialyltransferases (ST3Gals). In this study, we sought to elucidate the role of ST3GalIV in the biosynthesis of SLeX and in malignant properties of gastrointestinal (GI) cancer cells. By immunofluorescent screening, we selected SLeX-positive GI cancer cell lines and silenced ST3GalIV expression via CRISPR/Cas9. Flow cytometry, immunofluorescence and western blot analysis showed that ST3GalIV KO efficiently impaired SLeX expression in most cancer cell lines, with the exception of the colon cancer cell line LS174T. The impact of ST3GalIV KO in the biosynthesis of SLeX isomer SLeA and non sialylated Lewis X and A were also evaluated and overall, ST3GalIV KO led to a decreased expression of SLeA and an increased expression in both LeX and LeA. In addition, the abrogation of SLeX on GI cancer cells led to a reduction in cell motility. Furthermore, ST3GalVI KO was performed in LS174T ST3GalIV KO cells, resulting in the complete abolishment of SLeX expression and consequent reduced motility capacity of those cells. Overall, these findings portray ST3GalIV as the main, but not the only, enzyme driving the biosynthesis of SLeX in GI cancer cells, with a functional impact on cancer cell motility.","PeriodicalId":12762,"journal":{"name":"Glycoconjugate Journal","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10335957/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10584033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mass photometry: A powerful tool for carbohydrates-proteins conjugation monitoring and glycoconjugates molecular mass determination. 质谱法:用于碳水化合物-蛋白质偶联监测和糖缀合物分子质量测定的强大工具。

IF 3 4区生物学

Glycoconjugate Journal Pub Date : 2023-08-01 DOI: 10.1007/s10719-023-10126-7

Di Wu, Peng Xu, Meagan Kelly, Edward T Ryan, Pavol Kováč, Grzegorz Piszczek

{"title":"Mass photometry: A powerful tool for carbohydrates-proteins conjugation monitoring and glycoconjugates molecular mass determination.","authors":"Di Wu, Peng Xu, Meagan Kelly, Edward T Ryan, Pavol Kováč, Grzegorz Piszczek","doi":"10.1007/s10719-023-10126-7","DOIUrl":"https://doi.org/10.1007/s10719-023-10126-7","url":null,"abstract":"Glycoconjugate vaccines are important additions to the existing means for prevention of diseases caused by bacterial and viral pathogens. Conjugating carbohydrates to proteins is a crucial step in the development of these vaccines. Traditional mass spectrometry techniques, such as MALDI-TOF and SELDI-TOF, have difficulties in detecting glycoconjugates with high molecular masses. Mass photometry (MP) is a single-molecule technique that has been recently developed, which allows mass measurements of individual molecules and generates mass distributions based on hundreds to thousands of these measurements. In this study, we evaluated the performance of MP in monitoring carbohydrate-protein conjugation reactions and characterization of conjugates. Three different glycoconjugates were prepared from carrier protein BSA, and one from a large protein complex, a virus capsid with 3.74 MDa molecular mass. The masses measured by MP were consistent with those obtained by SELDI-TOF-MS and SEC-MALS. The conjugation of BSA dimer to carbohydrate antigen was also successfully characterized. This study shows that the MP technique is a promising alternative to methods developed earlier for monitoring glycoconjugation reactions and characterization of glycoconjugates. It measures intact molecules in solution and it is highly accurate over a wide mass range. MP requires only a very small amount of sample and has no specific buffer constraints. Other MP advantages include minimal cost of consumables and rapid data collection and analysis. Its advantages over other methods make it a valuable tool for researchers in the glycoconjugation field.","PeriodicalId":12762,"journal":{"name":"Glycoconjugate Journal","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10374364/pdf/nihms-1914410.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10228308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The generation of 5-N-glycolylneuraminic acid as a consequence of high levels of reactive oxygen species. 由于活性氧含量高而产生的5- n -糖基神经氨酸。

IF 3 4区生物学

Glycoconjugate Journal Pub Date : 2023-08-01 DOI: 10.1007/s10719-023-10121-y

Ruifeng Bai, Jingyi Wang, Inka Brockhausen, Yin Gao

引用次数: 0