Fungal biology最新文献

{"title":"Penicillium rubens, a root fungal endophyte, increase stress tolerance and productivity in crops under drought stress","authors":"Cristian Atala ,&nbsp;Ian S. Acuña-Rodríguez ,&nbsp;Sebastián A. Reyes ,&nbsp;Marco A. Molina–Montenegro","doi":"10.1016/j.funbio.2025.101659","DOIUrl":"10.1016/j.funbio.2025.101659","url":null,"abstract":"<div><div>Mutualistic interactions with fungal endophytes are a promising strategy for enhancing plant stress tolerance and productivity. This study evaluated the impact of fungal endophytes on drought tolerance and productivity in lettuce (<em>Lactuca sativa</em>) and tomato (<em>Solanum lycopersicum</em>). Seedlings were grown under semi-controlled conditions with endophyte-treated (E+, inoculated with <em>Penicillium rubens</em>) and axenic (E−) plants subjected to three irrigation regimes. Productivity was measured as shoot biomass (lettuce) and fruit production (tomato). Drought tolerance was assessed via proline content, <em>NHX1</em> gene expression, and root biomass, summarized by an integrated tolerance index. Inoculated lettuce showed higher tolerance, productivity, and root biomass, with no trade-off between these traits. Inoculated tomatoes exhibited higher yields under both no-stress and severe-stress conditions, with overcompensation under high stress. The integrated tolerance index highlighted enhanced productivity and tolerance in inoculated plants, demonstrating fungal endophytes as a sustainable strategy to mitigate drought stress and maintain crop yields.</div></div>","PeriodicalId":12683,"journal":{"name":"Fungal biology","volume":"129 7","pages":"Article 101659"},"PeriodicalIF":3.0,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145045854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SNX-BAR protein StMvp1 is required for the endosomal sorting, melanin transport, and pathogenicity of Setosphaeria turcica","authors":"Tianyi Zhao,&nbsp;Jinzhuo Shi,&nbsp;Lu Zhang,&nbsp;Wei Yuan,&nbsp;Ning Liu,&nbsp;Zhiyan Cao,&nbsp;Jingao Dong","doi":"10.1016/j.funbio.2025.101653","DOIUrl":"10.1016/j.funbio.2025.101653","url":null,"abstract":"<div><div>The fungal pathogen <em>Setosphaeria turcica</em> causes northern corn leaf blight and relies on melanized appressoria for host invasion. SNX-BAR proteins are a subfamily of Sorting nexins (SNX), which regulate membrane trafficking, cargo sorting and membrane remodeling at the endosome. Previous studies have suggested that SNX-BARs play a critical role in growth, development and virulence of plant pathogens. However, its roles in <em>S. turcica</em> remain not fully understood. Here, we characterized the SNX-BAR protein StMvp1, a homolog of yeast Mvp1, in <em>S. turcica</em> and uncovered its critical role in melanin biosynthesis, autophagy, and pathogenicity. Deletion of <em>StMvp1</em> impaired polarized growth, and appressorium development, significantly reducing pathogenicity on maize. <em>StMvp1</em> located in endosomes and is necessary for endocytosis. Notably, Δ<em>StMvp1</em> accumulated intracellular melanin due to the mislocalization of key synthases (<em>StPKS18</em>, <em>StLac1</em>, and <em>StSCD3</em>), which were trapped in punctate endosomal compartments. Meanwhile, Δ<em>StMvp1</em> exhibited aberrant autophagosome formation and impaired autophagy-dependent appressorium maturation. Our study establishes <em>StMvp1</em> as a regulator of endosomal sorting, melanin transport, and pathogenicity, providing insights into SNX-BAR-mediated pathogenesis in plant pathogenic fungi.</div></div>","PeriodicalId":12683,"journal":{"name":"Fungal biology","volume":"129 7","pages":"Article 101653"},"PeriodicalIF":3.0,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145045852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An exploratory study on crack healing in cementitious matrices induced by Pythium aphanidermatum","authors":"Nahúm G. Cayo Chileno ,&nbsp;Daniela Sales Alviano ,&nbsp;Celuta Sales Alviano ,&nbsp;Tatiana Cardoso e Bufalo ,&nbsp;Talita Martins ,&nbsp;Laércio Mesquita Júnior ,&nbsp;Gabrielle Avelar Silva ,&nbsp;Maria Alves Ferreira ,&nbsp;Joaquin H. Aquino Rocha ,&nbsp;Otavio da Fonseca Martins Gomes ,&nbsp;Romildo Dias Toledo Filho ,&nbsp;Saulo Rocha Ferreira","doi":"10.1016/j.funbio.2025.101658","DOIUrl":"10.1016/j.funbio.2025.101658","url":null,"abstract":"<div><div>The biomineralization of calcium carbonate (CaCO₃) mediated by microorganisms has been extensively studied for decades, with a predominant focus on bacteria, algae, and fungi. However, the functional and operational limitations of these species highlight the need to investigate new biological agents. This study aims to evaluate the biomineralization potential of <em>Pythium aphanidermatum</em>, an oomycete from the Chromista kingdom, for crack-healing applications in cement mortars. Its ureolytic capacity to accelerate CaCO₃ formation was analyzed, and liquid cultures with controlled concentrations of cells and calcium acetate were designed. Additionally, the microorganism's growth in alkaline media was evaluated. A surface application technique was adapted to determine the crack repair potential in cementitious matrices. Results demonstrated that <em>P aphanidermatum</em> does not necessarily require urea to form CaCO₃. A concentration of 10⁵ cells/mL and 100 mM of calcium acetate were optimal for microorganism development through surface application and CaCO₃ formation. It was also observed that <em>P. aphanidermatum</em> can tolerate alkaline environments (pH 11). Finally, its filamentous growth allowed partial filling of cracks in carbonated cement mortars. This work expands the scope of biomineralization by incorporating an organism from a previously unreported kingdom into this field, laying the foundation for sustainable and innovative applications in the construction industry.</div></div>","PeriodicalId":12683,"journal":{"name":"Fungal biology","volume":"129 7","pages":"Article 101658"},"PeriodicalIF":3.0,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145010691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diversity of Translucidithyrium (Mycosphaerellales, Phaeothecoidiellaceae) in China: Insights into their evolutionary split time and cophylogeny","authors":"Jia-Yu Song ,&nbsp;Hai-Xia Wu ,&nbsp;Kevin D. Hyde ,&nbsp;Wei-Feng Ding ,&nbsp;Qiang Wang ,&nbsp;Cui-Ling Gong ,&nbsp;Yi-Tong Wang","doi":"10.1016/j.funbio.2025.101656","DOIUrl":"10.1016/j.funbio.2025.101656","url":null,"abstract":"<div><div>The species evolution of epiphytic fungi are unique and critically important due to their association with plants. In this study, six new species of <em>Translucidithyrium</em> were discovered re-integrated morphological characteristics of this genus combined with phylogenetic analyses. The phylogenetic analysis revealed that all species of <em>Translucidithyrium</em> formed a monophyletic clade within <em>Phaeothecoidiellaceae</em>. The molecular clock analysis indicated that the divergence time of extant species of <em>Translucidithyrium</em> occurred during the Cenozoic era (18 Mya). The cophylogeny was conducted based on separately phylogenetic trees of <em>Translucidithyrium</em> and its host plants to explore their evolutionary relationships. <em>Translucidithyrium</em>'s species with host plant selection likely being random. Understanding the evolutionary history of <em>Translucidithyrium</em> species provides new perspectives and data to support research into the evolution of epiphytic fungi.</div></div>","PeriodicalId":12683,"journal":{"name":"Fungal biology","volume":"129 7","pages":"Article 101656"},"PeriodicalIF":3.0,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145045853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diversity and temporal succession of early-colonizing fungi in wood baits from an estuarine environment, with description of Penicillium alavariense sp. nov","authors":"Alberto C. Abreu,&nbsp;Micael F.M. Gonçalves,&nbsp;Ana C. Esteves,&nbsp;Artur Alves","doi":"10.1016/j.funbio.2025.101645","DOIUrl":"10.1016/j.funbio.2025.101645","url":null,"abstract":"<div><div>Lignicolous marine fungi thrive in marine environments by colonizing wood, where they play a vital role in the degradation and recycling of complex molecules. However, lignicolous marine fungi remain under-described, with significant gaps in knowledge concerning their species diversity. To assess the diversity and temporal succession of lignicolous marine fungi, we submerged wood blocks of <em>Pinus pinaster</em> and <em>Fagus sylvatica</em> at the Ria de Aveiro estuary, Portugal and collected samples every two months, over six months. Twenty-nine fungal genera were identified from 251 isolates, with <em>Penicillium</em> being the most common genus after two months of block submersion. Wood type seems to have modulated fungal diversity, with several genera including <em>Lulworthia</em> spp. prefering beech wood, and <em>Paralulworthia</em> colonized pine. We showed that marine fungal genera from the family <em>Lulworthiaceae</em> are predominant and consistent colonizers of wood substrates in estuarine environments, occupying an intermediate role in colonization. In contrast, the genus <em>Penicillium</em>, the pioneer colonizer, decreases over time, likely due to competition with other marine genera. Based on multilocus phylogeny, using sequences of internal transcribed spacer region of the nuclear RNA gene cluster (ITS), and partial sequences of tubulin (<em>tub2</em>), calmodulin (<em>cal</em>) and RNA polymerase II (<em>rpb2</em>) coding genes’ sequences, and morphological data, we propose <em>Penicillium alavariense</em> sp. nov., as a novel species in the series <em>Simplicissima</em>. These findings provide new insights into fungal succession on wood substrates, highlighting the role of wood type in shaping fungal communities and advancing our understanding of nutrient cycling in estuarine ecosystems.</div></div>","PeriodicalId":12683,"journal":{"name":"Fungal biology","volume":"129 7","pages":"Article 101645"},"PeriodicalIF":3.0,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144933478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Wickerhamomyces anomalus is a predator of the Castanea spp. ink disease-causing oomycetes Phytophthora cinnamomi and P. xcambivora. Based on morphological evidence, a model illustrating a specific mode of action is provided","authors":"Mariana Amorim-Rodrigues ,&nbsp;Rogélio Lopes Brandão ,&nbsp;Fernanda Cássio ,&nbsp;Cândida Lucas","doi":"10.1016/j.funbio.2025.101663","DOIUrl":"10.1016/j.funbio.2025.101663","url":null,"abstract":"<div><div><em>Phytophthora cinnamomi</em> and <em>P.</em> x<em>cambivora</em> are serious phytopathogens, namely causing root rot/ink in chestnut trees, which severely threatens this historical crop. Oomycetes produce mycelium but are hard to control with traditional fungicides or BCAs. Recent research revealed <em>Wickerhamomyces anomalus</em>, a yeast endophyte, as a strong antagonist of key pre- and post-harvest fungal pathogens in laboratory settings. This study shows it also inhibits <em>Phytophthora</em> growth. Microscopy revealed that <em>W. anomalus</em> cells adhere to and are found inside <em>Phytophthora</em> hyphae, accumulating within collapsed areas, possibly nourishing on hyphal contents. These interactions occur without the intervention of volatile compounds, siderophores, or hydrolytic enzymes, leaving hyphal walls intact. SEM and TEM of hyphae from <em>P. cinnamomi</em> when co-cultured with <em>W. anomalus</em> showed numerous intrahyphal structures formed in response to the yeast-imposed stress. Otherwise, the yeast shows polarised cells, nuclei fusion, irregularly numbered spores and intercellular bridges, indicating a disrupted reproductive cycle. This is consistent with <em>P. cinnamomi</em> and <em>W. anomalus</em> recognising each other's pheromones, triggering a mating-like response leading to the yeast's attachment and internalisation without damaging the hyphal cell wall. This <em>W. anomalus</em> specific mode-of-action, different from the ones previously reported, <em>s</em>uggests potential as a BCA for the pre-harvest management of <em>Phytophthora</em>.</div></div>","PeriodicalId":12683,"journal":{"name":"Fungal biology","volume":"129 7","pages":"Article 101663"},"PeriodicalIF":3.0,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145216744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of the OTAbZIP gene in regulating growth and OTA production in Aspergillus westerdijkiae fc-1 under osmotic stress","authors":"Yanling Ma ,&nbsp;Muyuan Zhuang ,&nbsp;Tanvir Ahmad ,&nbsp;Yuhong Yan ,&nbsp;Weitian Yuan ,&nbsp;Mingxuan Li ,&nbsp;Guangyou Tan ,&nbsp;Yingyao Deng ,&nbsp;Yang Liu","doi":"10.1016/j.funbio.2025.101662","DOIUrl":"10.1016/j.funbio.2025.101662","url":null,"abstract":"<div><div>Ochratoxin A (OTA), a potent carcinogenic mycotoxin, is produced by fungi from the <em>Aspergillus</em> and <em>Penicillium</em> genera. Reducing OTA contamination in food remains a global challenge. Osmotic stress is a key factor that influencing fungal development and secondary metabolism, particularly in <em>Aspergillus</em> species. In this study, we explored regulatory role of the <em>OTAbZIP</em> gene, a bZIP-type transcription factor, in growth and OTA biosynthesis under varying NaCl-induced osmotic conditions. Using RNA-Seq and RT-qPCR, we analyzed the transcriptomic responses of wild-type (WT) and <em>ΔOTAbZIP</em> mutant strains of <em>Aspergillus westerdijkiae</em> fc-1 exposed to 0, 20, and 100 g/L NaCl. Deletion of <em>OTAbZIP</em> significantly altered the expression of genes involved in DNA replication, sugar metabolism, ribosome function, and arginine and proline metabolism. The <em>ΔOTAbZIP</em> mutant exhibited greater sensitivity to osmotic stress and lower expression of OTA biosynthetic genes (<em>otaA–D</em>) than the WT. Although components of the HOG-MAPK pathway (<em>Hog1</em>, <em>Gpd1</em>, <em>Cdc28</em>, and <em>Ctt1</em>) were differentially expressed, <em>OTAbZIP</em> deletion did not block <em>Hog1</em> activation, suggesting <em>OTAbZIP</em> may function in a distinct regulatory role upstream or parallel to this pathway. Overall, our findings highlight <em>OTAbZIP</em> as a key regulator of osmotic stress response and OTA biosynthesis in <em>A. westerdijkiae</em>, offering potential molecular targets to reduce OTA contamination in food and feed.</div></div>","PeriodicalId":12683,"journal":{"name":"Fungal biology","volume":"129 7","pages":"Article 101662"},"PeriodicalIF":3.0,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145216745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Around the world in eight white rot species: Assessment of enzymatic and non-enzymatic wood decay pathways of worldwide Esca Complex of Diseases (ECD) associated basidiomycetes","authors":"Alessandro Puca ,&nbsp;Samuele Moretti ,&nbsp;Mary-Lorène Goddard ,&nbsp;Jacques Lalevée ,&nbsp;Hanns-Heinz Kassemeyer ,&nbsp;Sibylle Farine ,&nbsp;Laura Mugnai ,&nbsp;Christophe Bertsch","doi":"10.1016/j.funbio.2025.101661","DOIUrl":"10.1016/j.funbio.2025.101661","url":null,"abstract":"<div><div>Esca Complex of Diseases (ECD) is widespread in viticultural areas worldwide, posing a serious risk to modern viticulture. The role of basidiomycetes in the onset of foliar symptoms has recently been reconsidered, highlighting a link between the reduction in these symptoms and the presence of <em>Fomitiporia mediterranea</em> in vines. As several basidiomycetes have been associated with wood decay in grapevine, understanding their degradation mechanisms could help unravel the role of wood degradation in ECD. Thus, a collection of ECD-associated basidiomycetes, including <em>F. capensis</em>, <em>F. langloisii</em>, <em>F. polymorpha</em>, <em>F. australiensis</em>, <em>Tropicoporus texanus</em>, <em>Inonotus vitis</em>, and <em>Stereum hirsutum</em>, was examined in addition to <em>F. mediterranea</em>. Our study investigates the production and activity of lignocellulosic enzymes (particularly class-II peroxidases and laccases), along with the low molecular weight compounds (LMWC) in the secretome of these fungi, to assess their ability to perform the Chelator-Mediated-Fenton (CMF) pathway. Results highlight the production of ligninolytic enzymes with low production of active manganese peroxidases and a virtually total absence of lignin peroxidase activity. Additionally, the study confirms that the CMF mechanism is widespread among these fungi, with all LMW secretomes fulfilling the required steps of the CMF mechanism. This study provides critical insights into the degradation strategies of ECD-associated white rot agents, offering potential new perspectives on studying ECD symptomatology and developing targeted control strategies. The findings emphasize the need to consider the CMF mechanism in the context of fungal wood degradation and its potential role in ECD development.</div></div>","PeriodicalId":12683,"journal":{"name":"Fungal biology","volume":"129 7","pages":"Article 101661"},"PeriodicalIF":3.0,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145266577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In silico search reveals the association of lichens with black yeast-like fungi in the order Chaetothyriales","authors":"Flavia de Fatima Costa ,&nbsp;Bruno Paulo Rodrigues Lustosa ,&nbsp;Camila Pereira Perico ,&nbsp;Ricardo Belmonte-Lopes ,&nbsp;João Lucas Vitório Ribeiro Carvalho ,&nbsp;Emanuel L. Razzolini ,&nbsp;Germana Davila dos Santos ,&nbsp;Bruna Jacomel Favoreto de Souza Lima ,&nbsp;Cristina Maria de Souza-Motta ,&nbsp;Roberto Tadeu Raittz ,&nbsp;Yinggai Song ,&nbsp;Laura Selbmann ,&nbsp;G. Sybren de Hoog ,&nbsp;Jacques Meis ,&nbsp;Vania Aparecida Vicente","doi":"10.1016/j.funbio.2025.101618","DOIUrl":"10.1016/j.funbio.2025.101618","url":null,"abstract":"<div><div>Lichens exemplify a unique symbiotic relationship between fungi and algae or cyanobacteria, where fungi (mycobionts) provide structural support, while algae or cyanobacteria (photobionts) provide nutrients. Recent discoveries in the order Chaetothyriales have led to the description of several lichenicolous species, underscoring an intricate relationship of some black yeast-like fungi with lichens. The present study aims to investigate public metagenomic data of lichens available in the SRA database, covering a total of 2888 samples. The analysis incorporated 122 molecular marker sequences (barcodes and padlock probes) previously documented in the literature for species classified within Chaetothyriales. Additionally, 11 novel barcodes for species recently identified in lichens of the genera <em>Cladophialophora</em> and <em>Paracladophialophora</em> are described. The selected metagenomes were then compared with molecular marker sequences using local BLASTn (v2.6.0+), considering only alignments with a coverage cut-off and 100 % identity (perfect match). Reads from each sample were retrieved from the SRA as a multifasta file and analyzed with the SWeeP method for vector-based, alignment-free sequence analysis. The analysis identified fungi that are known as environmental inhabitants and, occasionally, opportunistic pathogens of vertebrates, including species in the genera <em>Cladophialophora</em>, <em>Cyphellophora</em>, and <em>Exophiala</em>. These species were distributed across 11 BioProjects from various locations around the world. The findings of this study corroborate extant knowledge concerning fungal colonization in diverse extremophilic environments, including deserts, tundra, and rocky surfaces.</div></div>","PeriodicalId":12683,"journal":{"name":"Fungal biology","volume":"129 6","pages":"Article 101618"},"PeriodicalIF":2.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144312750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Myxomycetes in urban green space in subtropical China: Spatiotemporal patterns override forest type effects","authors":"Wen-Long Song ,&nbsp;Di Lin ,&nbsp;Min-Li Cai ,&nbsp;Xia Chen ,&nbsp;Qun Dai ,&nbsp;Shuang-Lin Chen","doi":"10.1016/j.funbio.2025.101632","DOIUrl":"10.1016/j.funbio.2025.101632","url":null,"abstract":"<div><div>Urban green areas are vital yet underexplored reservoirs of microbial diversity in cities. This study examines myxomycete communities in Zijin Mountain National Forest Park, a subtropical urban forest in Nanjing, China, across four seasons and multiple forest types. Combining field collections and moist chamber cultures, we documented 60 species from 906 occurrence records. Seasonal variation dominated community dynamics, with species richness peaking in summer and declining in winter. β-diversity was primarily driven by species turnover (<em>β</em>_<em>sim</em> = 0.23–0.28), with nestedness playing a minor role across temporal and spatial scales. <em>Ceratiomyxa fruticulosa</em> emerged as a forest-type indicator for mixed broad-leaved stands, while <em>Arcyria cinerea</em> and <em>Cribraria violacea</em> tracked seasonal humidity changes. Crucially, myxomycete composition showed no statistically significant differentiation among forest types, a departure from natural ecosystems, suggesting that urban management homogenizes habitats critical for microbial niche partitioning. These findings highlight urban parks’ underrecognized role in preserving myxomycete diversity but reveal their vulnerability to microhabitat simplification. We advocate conseration of decaying wood and leaf litter to maintain ecological functions, offering actionable strategies for biodiversity-informed urban planning.</div></div>","PeriodicalId":12683,"journal":{"name":"Fungal biology","volume":"129 6","pages":"Article 101632"},"PeriodicalIF":2.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144714531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}