Fungal biology最新文献_第7页

Individual peroxiredoxin or Tor pathway components are not required for circadian clock function in Neurospora crassa 粗神经孢子虫的生物钟功能不需要单独的过氧化物还氧蛋白或Tor通路成分

IF 2.9 3区生物学

Fungal biology Pub Date : 2025-10-01 Epub Date: 2025-06-27 DOI: 10.1016/j.funbio.2025.101619

Christina M. Kelliher , Jay C. Dunlap

{"title":"Individual peroxiredoxin or Tor pathway components are not required for circadian clock function in Neurospora crassa","authors":"Christina M. Kelliher , Jay C. Dunlap","doi":"10.1016/j.funbio.2025.101619","DOIUrl":"10.1016/j.funbio.2025.101619","url":null,"abstract":"<div><div>In many model organisms, the circadian system has been proposed to comprise multiple oscillators that interact to promote accuracy of the clock as well as intricacies of rhythmic outputs. In <em>Neurospora crassa</em>, the circadian transcriptional/translational loop comprising of the FRQ (Frequency) and WCC (White Collar Complex) proteins has been instrumental in explaining many attributes of the clock including entrainment and rhythms in development and gene expression; in addition, some non-circadian oscillations can be unmasked when the FRQ-WCC feedback loop is eliminated. These rhythms have often lost defining circadian characteristics and are potentially controlled by other oscillators, termed FRQ-less oscillators (FLOs) in <em>Neurospora</em>. Understanding the biology of these oscillators and their hierarchical relationship with the FRQ-WCC oscillator (FWO) are salient questions in rhythms research. In this study, we examined candidate FLO effector pathways involving peroxiredoxins (Prxs) and mTOR. We find that independent gene knockouts compromising each pathway do not alter circadian period length or decrease the amplitude of the core circadian FWO rhythm in any meaningful way in <em>Neurospora</em>. Our findings suggest that molecular rhythms in Prx oxidation and in mTOR activity on the <em>chol-1</em> FLO oscillator are neither required for nor strongly regulate FWO components during a normal circadian day.</div></div>","PeriodicalId":12683,"journal":{"name":"Fungal biology","volume":"129 6","pages":"Article 101619"},"PeriodicalIF":2.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144535417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring vitamin D3 profile of epiphytic lichen forming fungi in forest ecosystems: Influence of habitat-dependent ecological variables 探索森林生态系统中附生地衣形成真菌的维生素D3谱：生境依赖生态变量的影响

IF 3 3区生物学

Fungal biology Pub Date : 2025-10-01 Epub Date: 2025-07-29 DOI: 10.1016/j.funbio.2025.101634

Gülşah Çobanoğlu , Hilmi Özdemir , Mehmet Özdemir , Gafura Aylak Özdemir , Emrah Özdemir , Fatma Ebru Koç , Ahmet Özcan

{"title":"Exploring vitamin D3 profile of epiphytic lichen forming fungi in forest ecosystems: Influence of habitat-dependent ecological variables","authors":"Gülşah Çobanoğlu , Hilmi Özdemir , Mehmet Özdemir , Gafura Aylak Özdemir , Emrah Özdemir , Fatma Ebru Koç , Ahmet Özcan","doi":"10.1016/j.funbio.2025.101634","DOIUrl":"10.1016/j.funbio.2025.101634","url":null,"abstract":"<div><div>Vitamin D is critically important for sustainable human health, and the rising prevalence of deficiency-related diseases has increased interest in natural sources. This study explores the potential of epiphytic lichen-forming fungi, known for their unique metabolites, as a novel biosource of vitamin D<sub>3</sub> for pharmaceutical and nutraceutical applications. Fourteen epiphytic lichen species were collected using a stratified sampling method from four mountainous forests in the Marmara Region of Türkiye. Vitamin D<sub>3</sub> contents of the samples were analysed in relation to six ecological variables: study area layer, stand type, tree species, altitude, aspect, and air humidity and temperature. Extraction methods including maceration, Soxhlet, and supercritical CO<sub>2</sub> techniques were applied, followed by HPLC analysis. Olive oil-based maceration was identified as the most efficient extraction method. One-way ANOVA showed significant associations between vitamin D<sub>3</sub> content and lichen species (p = 0.024), layer, stand, and tree species. Concentrations ranged from 0.6 to 20.33 μg/g, with <em>Bryoria fuscescens</em>, <em>Evernia prunastri</em>, and <em>Pseudevernia furfuracea</em> yielding the highest values. The highest vitamin D<sub>3</sub> levels were detected in Uludağ, especially in coniferous forests dominated by <em>Pinus nigra</em>. The results indicated that forest layer, stand, altitude, and tree species significantly influence vitamin D<sub>3</sub> amounts, while aspect and humidity do not. This is the first study to comprehensively report natural vitamin D<sub>3</sub> content in lichens, filling an important scientific gap and demonstrating their potential as an eco-sustainable resource for vitamin D<sub>3</sub> production</div></div>","PeriodicalId":12683,"journal":{"name":"Fungal biology","volume":"129 6","pages":"Article 101634"},"PeriodicalIF":3.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144724675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fungal spore calendar for the warm temperate climate zone. What else besides Cladosporium spores? 暖温带气候区真菌孢子日历。除了枝孢子菌还有什么？

IF 3 3区生物学

Fungal biology Pub Date : 2025-10-01 Epub Date: 2025-08-20 DOI: 10.1016/j.funbio.2025.101641

Magdalena Wójcik-Kanach, Idalia Kasprzyk

{"title":"Fungal spore calendar for the warm temperate climate zone. What else besides Cladosporium spores?","authors":"Magdalena Wójcik-Kanach, Idalia Kasprzyk","doi":"10.1016/j.funbio.2025.101641","DOIUrl":"10.1016/j.funbio.2025.101641","url":null,"abstract":"<div><div>The qualitative and quantitative composition of airborne fungal spores results from the interaction of fungal biology, environmental factors, particularly climate, weather conditions, vegetation, land cover and human activity. Continuous aeromycological monitoring is rarely conducted due to the challenges associated with identifying the abundance of spores present in the air. In southeastern Poland such studies have been conducted only occasionally. Therefore, the aim of this research was to update the spore calendar with data on previously unstudied spore taxa. Using the volumetric method, the occurrence of 27 spore taxa was analyzed over a five-year period (2019–2023). Throughout the entire season, first- and second-order temporal compositional clusters were distinguished and characterized based on their distinctive spores. At the beginning of the season, Diatrypaceae and Pezizaceae spores were the most prominent. Airborne <em>Periconia</em>, <em>Arthrinium</em>, and Boletus type spores appeared in higher concentrations toward the end of the growing season. Although <em>Cladosporium</em> spores were definitely dominant in the air, Leptosphaeria type, <em>Ganoderma, Alternaria</em>, Pezizaceae, <em>Botrytis</em>, and <em>Epicoccum</em> spores should be considered a significant component of aeroplankton in the temperate climate zone. A comparison of current data with archived records suggests that fungi may exhibit diverse responses to significant increases in air temperature.</div></div>","PeriodicalId":12683,"journal":{"name":"Fungal biology","volume":"129 6","pages":"Article 101641"},"PeriodicalIF":3.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144893179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The transcription factor MrbHLH2 significantly affects stress resistance and insect virulence in Metarhizium robertsii 转录因子MrbHLH2显著影响罗伯特绿僵菌的抗逆性和昆虫毒力

IF 3 3区生物学

Fungal biology Pub Date : 2025-10-01 Epub Date: 2025-08-05 DOI: 10.1016/j.funbio.2025.101636

Yifan Kong , Yang Yang , Shaoxiong Zhu, Limei Yang, Bo Huang

{"title":"The transcription factor MrbHLH2 significantly affects stress resistance and insect virulence in Metarhizium robertsii","authors":"Yifan Kong , Yang Yang , Shaoxiong Zhu, Limei Yang, Bo Huang","doi":"10.1016/j.funbio.2025.101636","DOIUrl":"10.1016/j.funbio.2025.101636","url":null,"abstract":"<div><div>Basic helix-loop-helix (bHLH) transcription factors are essential regulators of various biological processes, including growth, development, and stress responses in eukaryotes. Despite their importance, the specific roles of bHLH factors in entomopathogenic fungi remain inadequately understood. In this study, we identified and characterized the bHLH transcription factor MrbHLH2 in the entomopathogenic fungus <em>Metarhizium robertsii</em>, which is widely used in biological control. Subcellular localization studies confirmed that MrbHLH2 is predominantly located in the nuclei of conidia. The deletion of <em>MrbHLH2</em> (Δ<em>MrbHLH2</em>) resulted in enhanced tolerance to osmotic and heat stress, while simultaneously decreasing tolerance to ultraviolet (UV) radiation. Notably, Δ<em>MrbHLH2</em> exhibited significantly reduced virulence, as indicated by a 0.83-day increase in LT<sub>50</sub> compared to the wild-type strain, along with delayed appressorium formation, impaired cuticle penetration and downregulation of several virulence-related genes. This study contributes to the understanding of bHLH transcription factors in entomopathogenic fungi and underscores the potential of targeting these factors to enhance the biocontrol efficacy of fungal pathogens.</div></div>","PeriodicalId":12683,"journal":{"name":"Fungal biology","volume":"129 6","pages":"Article 101636"},"PeriodicalIF":3.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144779658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cyanidin 3-O-glucoside and other anthocyanins affect enniatins production in Fusarium avenaceum 花青素- 3- o -葡萄糖苷和其他花青素影响镰刀菌多年生素的产生

IF 3 3区生物学

Fungal biology Pub Date : 2025-10-01 Epub Date: 2025-08-09 DOI: 10.1016/j.funbio.2025.101640

Linda Felici , Vessela Atanasova , Nadia Ponts , Christine Ducos , Sara Francesconi , Francesco Sestili , Florence Richard-Forget , Giorgio Mariano Balestra

{"title":"Cyanidin 3-O-glucoside and other anthocyanins affect enniatins production in Fusarium avenaceum","authors":"Linda Felici , Vessela Atanasova , Nadia Ponts , Christine Ducos , Sara Francesconi , Francesco Sestili , Florence Richard-Forget , Giorgio Mariano Balestra","doi":"10.1016/j.funbio.2025.101640","DOIUrl":"10.1016/j.funbio.2025.101640","url":null,"abstract":"<div><div>Fusarium Head Blight (FHB), caused by various <em>Fusarium</em> species, is a major threat to global cereal production. <em>F. avenaceum</em> is an important FHB pathogen producing enniatin mycotoxins. While several studies have explored the antifungal and anti-mycotoxigenic potential of different phenolic compounds, the effects of anthocyanins (pigments abundant in plants including certain cereals) remain poorly understood. This study explored the effects of anthocyanins on <em>F. avenaceum</em> conidial germination, fungal biomass and enniatins production. <em>In vitro</em> assays revealed that while anthocyanidins (cyanidin, delphinidin) inhibited conidial germination, cyanidin 3-<em>O</em>-glucoside and delphinidin 3-<em>O</em>-glucoside increased fungal biomass. Notably, cyanidin 3-<em>O</em>-glucoside and delphinidin consistently reduced enniatins production (A1, B and B1), whereas delphinidin 3-<em>O</em>-glucoside primarily reduced enniatin A1. These contrasting effects can likely be ascribed to presence or absence of the glycosylated moiety, distinct degradation pathways, and the influence of anthocyanins on fungal gene expression, including the regulation of key genes involved in enniatin biosynthesis and oxidative homeostasis. Furthermore, the study examined the concurrent effects of cyanidin 3-<em>O</em>-glucoside and ferulic acid, another plant phenolic compound. When combined, ferulic acid stabilized cyanidin 3-<em>O</em>-glucoside, preserving its anti-mycotoxin activity, while antifungal potential of ferulic acid was abrogated, highlighting the complex interactions between these compounds. These findings provide valuable insights into the potential of anthocyanins as natural anti-mycotoxin agents and suggest an optimization of their use for mycotoxin control.</div></div>","PeriodicalId":12683,"journal":{"name":"Fungal biology","volume":"129 6","pages":"Article 101640"},"PeriodicalIF":3.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144866028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Agaricus bisporus mushrooms are supplied with water through both apoplastic as well as symplastic routes from distinct substrate layers 双孢蘑菇通过不同基质层的外质体和共质体途径提供水分

IF 3 3区生物学

Fungal biology Pub Date : 2025-10-01 Epub Date: 2025-07-17 DOI: 10.1016/j.funbio.2025.101623

Koen C. Herman , Guus van Iersel , Jan Dijksterhuis , Han A.B. Wösten , Robert-Jan Bleichrodt

{"title":"Agaricus bisporus mushrooms are supplied with water through both apoplastic as well as symplastic routes from distinct substrate layers","authors":"Koen C. Herman , Guus van Iersel , Jan Dijksterhuis , Han A.B. Wösten , Robert-Jan Bleichrodt","doi":"10.1016/j.funbio.2025.101623","DOIUrl":"10.1016/j.funbio.2025.101623","url":null,"abstract":"<div><div><em>Agaricus bisporus</em> is grown commercially on compost topped with a peat-based casing layer. Water is translocated from compost and casing to enable formation of mushrooms. Here, water translocation from casing and different parts of the compost into mushrooms was studied and linked to their water potential and contributing factors thereof: i.e. osmotic- and matric potentials. Water in the mushrooms mainly originated from the casing and to a lesser extent from the top and middle layers of the compost. Based on these results, alternative casing regimes were tested to increase mushroom production. This resulted in a total yield increase of up to 1.4-fold when the old casing was topped with a fresh layer of casing or when the casing was replaced after harvesting the second flush. Data indicate that the difference in water potential can drive passive water translocation from the casing to the first flush, without expending cellular energy. However during the second flush, no such water potential gradient is established between casing and the mushrooms, yet mushrooms still develop. The water potential in the compost indicates that water cannot be translocated to the mushrooms at all. Therefore, other components of the water potential should drive this water flow. We have indications that this component is the turgor of the mycelium. Moreover, we found a novel route of water translocation from casing to mushrooms via the apoplast.</div></div>","PeriodicalId":12683,"journal":{"name":"Fungal biology","volume":"129 6","pages":"Article 101623"},"PeriodicalIF":3.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144722859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ZnCl2 inhibits postharvest disease on pear and crabapple by inducing autophagy of Penicillium expansum ZnCl2通过诱导膨胀青霉自噬抑制梨和海棠采后病害

IF 3 3区生物学

Fungal biology Pub Date : 2025-10-01 Epub Date: 2025-07-31 DOI: 10.1016/j.funbio.2025.101635

Jinyue Sun , Jia-Neng Pan , Tianzhu Zhang , Xiaodong Zheng , Wen-Wen Zhou

{"title":"ZnCl2 inhibits postharvest disease on pear and crabapple by inducing autophagy of Penicillium expansum","authors":"Jinyue Sun , Jia-Neng Pan , Tianzhu Zhang , Xiaodong Zheng , Wen-Wen Zhou","doi":"10.1016/j.funbio.2025.101635","DOIUrl":"10.1016/j.funbio.2025.101635","url":null,"abstract":"<div><div><em>Penicillium expansum</em> is a phytopathogen causing postharvest disease of many fruits, which has led to enormous losses. Therefore, it is of great significance to take efficient methods to control this notorious phytopathogen. In this study, zinc, an essential trace element for human body, has been found to be able to effectively inhibit the <em>P. expansum</em> mycelial growth on PDA and burst of ROS in the fungal hyphae. Additionally, the transmission electron microscope (TEM) analysis indicated that zinc induces autophagy and autophagic vacuoles in <em>P. expansum</em>. Furthermore, the zinc inhibits the activity of six key enzymes (PDH, SDH, CS, IDH, MDH, KGDH) in TCA pathway to inhibit the growth of <em>P. expansum</em>. The decrease of ATPase activity indicated that the function of mitochondria in <em>P. expansum</em> was destroyed after the treatment of zinc. Finally, the sodium alginate (SA)@ZnCl<sub>2</sub> coatings were verified to be effective in inhibiting the postharvest disease of pear and crabapple. Collectively, all the above results showed that zinc had great efficacy in suppressing <em>P. expansum</em> on fruit surfaces by inducing autophagy.</div></div>","PeriodicalId":12683,"journal":{"name":"Fungal biology","volume":"129 6","pages":"Article 101635"},"PeriodicalIF":3.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144772526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Moh1 coordinates ROS-dependent apoptosis in genotoxic stress response of Candida albicans Moh1在白色念珠菌基因毒性应激反应中协调ros依赖性凋亡

IF 3 3区生物学

Fungal biology Pub Date : 2025-10-01 Epub Date: 2025-08-21 DOI: 10.1016/j.funbio.2025.101642

Jia Wang, Wenxia Gao, Xinyi Tang, Jinrong Feng

{"title":"Moh1 coordinates ROS-dependent apoptosis in genotoxic stress response of Candida albicans","authors":"Jia Wang, Wenxia Gao, Xinyi Tang, Jinrong Feng","doi":"10.1016/j.funbio.2025.101642","DOIUrl":"10.1016/j.funbio.2025.101642","url":null,"abstract":"<div><div><em>Candida albicans</em> employs apoptosis to maintain genomic stability under genotoxic stress, yet its regulatory mechanisms remain poorly defined. Here, we characterize the role of a putative pro-apoptotic factor Moh1 in <em>C. albicans</em>. Our findings reveal that <em>MOH1</em> transcription is significantly upregulated under genotoxic stress and in DNA repair-deficient backgrounds (e.g., <em>rad52Δ</em>). Paradoxically, <em>MOH1</em> deletion enhances resistance to genotoxic agents, improving cellular survival and reducing apoptosis, as evidenced by decreased nuclear condensation. Mechanistically, <em>moh1Δ</em> cells exhibit reduced intracellular reactive oxygen species (ROS), and pharmacological ROS scavenging with N-acetylcysteine abolishes the stress-resistant phenotype. Genetic interaction analysis identifies potential functional overlap between <em>MOH1</em> and <em>NMA111</em>, a previously characterized pro-apoptotic factor. Transcriptomic profiling of <em>moh1Δ</em> cells under genotoxic stress shows dysregulation of DNA repair (<em>SPO11</em>, <em>RFC52</em>) and apoptosis (<em>Orf19.2175)</em> genes. While dispensable for hyphal morphogenesis and virulence in a <em>Galleria mellonella</em> model, Moh1 negatively regulates biofilm formation. Collectively, these findings establish Moh1 as a critical regulator of ROS-dependent apoptosis during genotoxic stress response in <em>C. albicans</em>, offering insights for targeting fungal apoptotic pathways in antifungal strategies.</div></div>","PeriodicalId":12683,"journal":{"name":"Fungal biology","volume":"129 6","pages":"Article 101642"},"PeriodicalIF":3.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144896511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The nitrogen regulator AreA modulates lipid metabolism through uga2 in Mucor circinelloides 氮调节剂AreA通过uga2调节毛霉的脂质代谢

IF 2.9 3区生物学

Fungal biology Pub Date : 2025-10-01 Epub Date: 2025-06-11 DOI: 10.1016/j.funbio.2025.101616

Xiuwen Wang , Hassan Mohamed , Qing Liu , Zhaosen Fan , Yuanda Song

{"title":"The nitrogen regulator AreA modulates lipid metabolism through uga2 in Mucor circinelloides","authors":"Xiuwen Wang , Hassan Mohamed , Qing Liu , Zhaosen Fan , Yuanda Song","doi":"10.1016/j.funbio.2025.101616","DOIUrl":"10.1016/j.funbio.2025.101616","url":null,"abstract":"<div><div>Oleaginous filamentous fungus <em>Mucor circinelloides</em> harbors a GATA transcription activator AreA, which regulates nitrogen metabolism. In our previous study, deletion of AreA resulted in increased lipid production, while its overexpression reduced lipid synthesis. Although it is not a direct lipogenesis regulator, AreA influences metabolic flux by modulating nitrogen utilization pathways, which in turn affects carbon distribution. One such pathway is the GABA shunt, an alternative route that bypasses two steps of TCA cycle and contributes to replenishing TCA intermediates under nitrogen-limited conditions. The <em>uga2</em> gene, encoding succinate semialdehyde dehydrogenase, plays a key role in this shunt. We constructed UGA2-knockout strain in wild-type, AreA-knockout and AreA-overexpression strains, respectively. Results showed that single knockout of <em>uga2</em> increased fatty acid content from 24.5 % to 32.7 %, while double knockout of <em>areA</em> and <em>uga2</em> exhibited the highest fatty acid content at 36.1 %. The <em>areA</em> overexpression combined with <em>uga2</em> knockout also resulted in increased lipid accumulation up to 32.4 %. The gene expression levels and enzyme activities related to fatty acid synthesis showed that deletion of <em>uga2</em> may lead to carbon-nitrogen metabolism disequilibrium, shifting carbon flux towards lipogenesis. This indicated that AreA might regulate lipid metabolism through the modulation of <em>uga2</em> in <em>M. circinelloides</em>.</div></div>","PeriodicalId":12683,"journal":{"name":"Fungal biology","volume":"129 6","pages":"Article 101616"},"PeriodicalIF":2.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144279426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Two homeobox transcription factors CgrHtf1 and CgrAfh1 hierarchically regulate asexual sporulation and appressorium formation in the maize anthracnose fungus Colletotrichum graminicola. 两个同源盒转录因子CgrHtf1和CgrAfh1在玉米炭疽病真菌Colletotrichum graminicola中分层调节无性孢子形成和附着胞形成。

IF 3 3区生物学

Fungal biology Pub Date : 2025-10-01 Epub Date: 2025-08-05 DOI: 10.1016/j.funbio.2025.101637

Shuangzhen Zhou, Chenchen Guo, Wen Tang, Zhiqiang Liu, Xiaoyu Li

{"title":"Two homeobox transcription factors CgrHtf1 and CgrAfh1 hierarchically regulate asexual sporulation and appressorium formation in the maize anthracnose fungus Colletotrichum graminicola.","authors":"Shuangzhen Zhou, Chenchen Guo, Wen Tang, Zhiqiang Liu, Xiaoyu Li","doi":"10.1016/j.funbio.2025.101637","DOIUrl":"10.1016/j.funbio.2025.101637","url":null,"abstract":"<p><p>Maize anthracnose, caused by the fungal pathogen Colletotrichum graminicola, is among the most devastating diseases affecting maize production. Homeobox transcription factors (HTFs) regulate key developmental and physiological processes in eukaryotes, including fungal pathogenesis. In this study, we identified two HTFs, CgrHtf1 and CgrAfh1, in C. graminicola. Both CgrHtf1 and CgrAfh1 contain a conserved homeobox domain and exhibit distinct nuclear localization, consistent with their predicted roles as transcriptional regulators. Disruption of Cgrhtf1 sharply reduced conidial production while increasing melanin biosynthesis. In contrast, deletion of Cgrafh1 not only impaired conidiation but also abolished the formation of functional appressoria and hyphopodia. Notably, overexpression of Cgrafh1 enhanced appressorium formation compared to the wild-type strain, suggesting its crucial role in the morphogenesis of appressoria. Transcriptome analysis revealed that CgrHtf1 regulates many genes associated with melanin biosynthesis, fungal development and cell cycle control, while CgrAfh1 predominantly modulates the expression of genes linked to signal transduction, cell cycle progression and autophagy processes. Collectively, we demonstrate that CgrHtf1 controls conidiation and melanin biosynthesis, whereas CgrAfh1 governs appressorium development, revealing hierarchical regulation of infection-related morphogenesis in C. graminicola.</p>","PeriodicalId":12683,"journal":{"name":"Fungal biology","volume":"129 6","pages":"101637"},"PeriodicalIF":3.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145015001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0