Fungal biology最新文献

{"title":"Unsaturated fatty acid, Nonacosenoic acid isolated from an endophyte Chaetomium nigricolor inhabiting the stem of Catharanthus roseus and its bioactivity","authors":"Geethanjali Dhayanithy ,&nbsp;Somnath Mukherjee ,&nbsp;Kamalraj Subban ,&nbsp;Subathra Radhakrishnan ,&nbsp;Jayabaskaran Chelliah","doi":"10.1016/j.funbio.2024.05.008","DOIUrl":"https://doi.org/10.1016/j.funbio.2024.05.008","url":null,"abstract":"<div><p>The endophytic fungus <em>Chaetomium nigricolor</em> culture filtrate's hexane extract was used to identify a cytotoxic very long-chain fatty acid. Based on multiple spectroscopic investigations, the structure of the compound was predicted to be an unsaturated fatty acid, Nonacosenoic acid (NA). Using the MTT assay, the compound's cytotoxic potential was evaluated against MCF-7, A-431, U-251, and HEK-293 T cells. The compound was moderately cytotoxic to breast carcinoma cell line, MCF-7 cells and negligibly cytotoxic to non-cancerous cell line HEK-293 T cells. The compound exhibited mild cytotoxic activity against A-431 and U-251 cells. The compound also induced ROS generation and mitochondrial depolarization in MCF-7 cells when assessed via the NBT and JC-1 assays, respectively. This is the first report on the production of nonacosenoic acid from the endophytic fungus <em>Chaetomium nigricolor</em> and the assessment of its bioactivity.</p></div>","PeriodicalId":12683,"journal":{"name":"Fungal biology","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141239855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The chemotrophic behaviour of Aspergillus niger: Mapping hyphal filaments during chemo-sensing; the first step towards directed materials formation","authors":"Ayesha Sadaf ,&nbsp;Matthias Brock ,&nbsp;Carole C. Perry","doi":"10.1016/j.funbio.2024.05.010","DOIUrl":"https://doi.org/10.1016/j.funbio.2024.05.010","url":null,"abstract":"<div><p>In the development of fungal based materials for applications in construction through to biomedical materials and fashion, understanding how to regulate and direct growth is key for gaining control over the form of material generated. Here, we show how simple ‘chemical food’ cues can be used to manipulate the growth of fungal networks by taking <em>Aspergillus niger</em> as an exemplar species. Chemotrophic responses towards a range of nitrogen and carbon containing biomolecules including amino acids, sugars and sugar alcohols were quantified in terms of chemotrophic index (CI) under a range of basal media compositions (low and high concentrations of N and C sources). Growth of filamentous networks was followed using fluorescence microscopy at single time points and during growth by an AI analytical approach to explore chemo sensing behaviour of the fungus when exposed to pairs (C-C, C-N, N-N) of biomolecules simultaneously. Data suggests that the directive growth of <em>A. niger</em> can be controlled towards simple biomolecules with CI values giving a good approximation for expected growth under a range of growth conditions. This is a first step towards identifying conditions for researcher-led directed growth of hyphae to make mycelial mats with tuneable morphological, physicochemical, and mechanical characteristics.</p></div>","PeriodicalId":12683,"journal":{"name":"Fungal biology","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1878614624000692/pdfft?md5=124412f3efff561b703406230495f5c2&pid=1-s2.0-S1878614624000692-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141239854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fungal colonization and penetration of mortar as a suitable simulant for concrete: Implications for fungal biodeterioration in the built environment","authors":"Geoffrey Michael Gadd ,&nbsp;Louise McGregor","doi":"10.1016/j.funbio.2024.05.012","DOIUrl":"https://doi.org/10.1016/j.funbio.2024.05.012","url":null,"abstract":"<div><p>A range of fungal species showed variable abilities to colonize and penetrate a mortar substrate. Calcium biomineralization was a common feature with calcium-containing crystals deposited in the microenvironment or encrusting hyphae, regardless of the specific mortar composition. Several species caused significant damage to the mortar surface, exhibiting burrowing and penetration, surface etching, and biomineralization. In some cases, extensive biomineralization of hyphae, probably by carbonatization, resulted in the formation of crystalline tubes after hyphal degradation on mortar blocks, including those amended with Co or Sr carbonate. Ca was the only metal detected in the biomineralized formations with Co or Sr undetectable. <em>Aspergillus niger</em>, <em>Stemphylium</em> sp. and <em>Paecilomyces</em> sp. could penetrate mortar with differential responses depending on the porosity. Fluorescent staining of thin sections recorded penetration depths of ∼530 um for <em>A. niger</em> and ∼620 um for <em>Stemphylium</em> sp. Penetration depth varied inversely with porosity and greater penetration depths were achieved in mortar with a lower porosity (lower water/cement ratio). These results have provided further understanding of biodeteriorative fungal interactions with cementitious substrates that can clearly affect structural integrity. The potential significance of fungal colonization and such biodeteriorative phenomena should not be overlooked in built environment contexts, including radionuclide storage and surface decontamination.</p></div>","PeriodicalId":12683,"journal":{"name":"Fungal biology","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1878614624000710/pdfft?md5=732e6ce3764661ef337dfa86ea8db69c&pid=1-s2.0-S1878614624000710-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141291674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cordyceps cateniannulata: An endophyte of coffee, a parasite of coffee leaf rust and a pathogen of coffee pests","authors":"Caio M. Pereira ,&nbsp;Keminy R. Bautz ,&nbsp;María del Carmen H. Rodríguez ,&nbsp;Laura M. Saavedra-Tobar ,&nbsp;Miraine Kapeua-Ndacnou ,&nbsp;Kifle Belachew-Bekele ,&nbsp;Simon L. Elliot ,&nbsp;Harry C. Evans ,&nbsp;Robert W. Barreto","doi":"10.1016/j.funbio.2024.05.004","DOIUrl":"https://doi.org/10.1016/j.funbio.2024.05.004","url":null,"abstract":"<div><p>Here, we report on a <em>Cordyceps</em> species entering into a multi-trophic, multi-kingdom association. <em>Cordyceps cateniannulata</em>, isolated from the stem of wild <em>Coffea arabica</em> in Ethiopia, is shown to function as an endophyte, a mycoparasite and an entomopathogen. A detailed polyphasic taxonomic study, including a multilocus phylogenetic analysis, confirmed its identity. An emended description of <em>C. cateniannulata</em> is provided herein. Previously, this species was known as a pathogen of various insect hosts in both the Old and New World. The endophytic status of <em>C. cateniannulata</em> was confirmed by re-isolating it from inoculated coffee plants. Inoculation studies have further shown that <em>C. cateniannulata</em> is a mycoparasite of <em>Hemileia vastatrix</em>, as well as an entomopathogen of major coffee pests; infecting and killing <em>Hypothenemus hampei</em> and <em>Leucoptera coffeella</em>. This is the first record of <em>C. cateniannulata</em> from Africa, as well as an endophyte and a mycoparasite. The implications for its use as a biocontrol agent are discussed.</p></div>","PeriodicalId":12683,"journal":{"name":"Fungal biology","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141314409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exhaustive identification and characterization of Colletotrichum siamense and Colletotrichum fructicola as causative agents of circular leaf spot disease of rubber tree (Hevea brasiliensis) in India","authors":"V.K. Vineeth,&nbsp;Shilpa Babu,&nbsp;T.R. Reshma,&nbsp;Shaji Philip,&nbsp;Edwin Prem","doi":"10.1016/j.funbio.2024.05.009","DOIUrl":"10.1016/j.funbio.2024.05.009","url":null,"abstract":"<div><p>The rubber tree (<em>Hevea brasiliensis</em>) is one of the major domesticated crops planted commercially for the production of natural rubber (NR) worldwide. In recent years, rubber trees in the Southern states of India and other rubber-producing countries have experienced a severe leaf spot disease, characterized by the appearance of several brown circular spots in the initial stage, which later spread all over the lamina of fully matured leaves, leading to yellowing and defoliation. The causal organism of this Circular Leaf Spot (CLS) disease has not been conclusively identified in any previous studies. In this study, we collected infected leaf samples from various locations in the South Indian states. We aimed to identify the actual fungal pathogen that causes the CLS disease on rubber trees. Based on the morphological and molecular analysis of the most frequently isolated fungi from infected leaf samples were identified as <em>Colletotrichum siamense</em> and <em>Colletotrichum fructicola</em>. Pathogenicity tests also confirmed the involvement of isolated <em>Colletotrichum</em> spp. in the development of CLS disease. These findings provide valuable insights into understanding the CLS disease and its impact on rubber cultivation. To our knowledge, it is the first report of <em>C. siamense</em> and <em>C. fructicola</em> associated with CLS disease of rubber trees in India.</p></div>","PeriodicalId":12683,"journal":{"name":"Fungal biology","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141138543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Yeasts volatile organic compounds (VOCs) as potential growth enhancers and molds biocontrol agents of mushrooms mycelia","authors":"Alessandra Di Francesco ,&nbsp;Erica Moret ,&nbsp;Rudy Cignola ,&nbsp;Luca Garagozzo ,&nbsp;Emanuela Torelli ,&nbsp;Michele Di Foggia","doi":"10.1016/j.funbio.2024.05.007","DOIUrl":"10.1016/j.funbio.2024.05.007","url":null,"abstract":"<div><p>Volatile organic compounds (VOCs) produced by yeasts can positively affect crops, acting as antifungals or biostimulants. In this study, <em>Aureobasidium pullulans</em> and <em>Metschnikowia pulcherrima</em> were evaluated as potential antagonists of <em>Trichoderma</em> spp., common fungal pathogen in mushroom cultivation. To assess the biocontrol ability and biostimulant properties of the selected yeast species, <em>in vitro</em> co-culture and VOCs exposure assays were conducted. In both assays, VOCs produced by <em>Aureobasidium</em> spp. showed the stronger antifungal activity with a growth inhibition up to 30 %. This result was further confirmed by the higher volatilome alcohol content revealed by solid phase microextraction-gas chromatography mass spectrometry (SPME/GC–MS). Overall, <em>Aureobasidium</em> strains can be potentially used as biocontrol agent in <em>Pleorotus ostreatus</em> and <em>Cyclocybe cylindracea</em> mycelial growth, without affecting their development as demonstrated by VOCs exposure assay and Fourier-transform infrared spectroscopy (FT-IR). Conversely, <em>M. pulcherrima</em> was characterized by a lower or absent antifungal properties and by a volatilome composition rich in isobutyl acetate, an ester often recognized as plant growth promoter. As confirmed by FT-IR, <em>Lentinula</em> mycelia exposed to <em>M. pulcherrima</em> VOCs showed a higher content of proteins and lipids, suggesting an improvement of some biochemical properties. Our study emphasizes that VOCs produced by specific yeast strains are potentially powerful alternative to synthetic fungicide in the vegetative growth of mushroom-forming fungi and also able to modify their biochemical composition.</p></div>","PeriodicalId":12683,"journal":{"name":"Fungal biology","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141145010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antagonistic potential of endophytic fungal isolates of tomato (Solanum lycopersicum L.) fruits against post-harvest disease-causing pathogens of tomatoes: An in vitro investigation","authors":"Phathutshedzo Ramudingana ,&nbsp;Tshifhiwa Paris Mamphogoro ,&nbsp;Casper Nyaradzai Kamutando ,&nbsp;Martin Makgose Maboko ,&nbsp;Kedibone Yvonne Modika ,&nbsp;Kgantjie Walter Moloto ,&nbsp;Mapitsi Silvester Thantsha","doi":"10.1016/j.funbio.2024.05.006","DOIUrl":"https://doi.org/10.1016/j.funbio.2024.05.006","url":null,"abstract":"<div><p>Post-harvest decay of fresh agricultural produce is a major threat to food security globally. Synthetic fungicides, commonly used in practice for managing the post-harvest losses, have negative impacts on consumers’ health. Studies have reported the effectiveness of fungal isolates from plants as biocontrol agents of post-harvest diseases, although this is still poorly established in tomatoes (<em>Solanum lycopersicum</em> L. cv. Jasmine). In this study, 800 endophytic fungi were isolated from mature green and ripe untreated and fungicide-treated tomato fruits grown in open soil and hydroponics systems. Of these, five isolates (<em>Aureobasidium pullulans</em> SUG4.1, <em>Coprinellus micaceus</em> SUG4.3, <em>Epicoccum nigrum</em> SGT8.6, <em>Fusarium oxysporum</em> HTR8.4, <em>Preussia africana</em> SUG3.1) showed antagonistic properties against selected post-harvest pathogens of tomatoes (<em>Alternaria alternata</em>, <em>Fusarium solani</em>, <em>Fusarium oxysporum</em>, <em>Geotrichum candidum</em>, <em>Rhizopus stolonifera, Rhizoctonia solani</em>), with <em>Lactiplantibacillus plantarum</em> as a positive control. <em>P. africana</em> SUG3.1 and <em>C. micaceus</em> SUG4.3 significantly inhibited growth of all the pathogens, with antagonistic capabilities comparable to that exhibited by <em>L. plantarum</em>. Furthermore, the isolates produced an array of enzymes, including among others, amylase, cellulose and protease; and were able to utilize several carbohydrates (glucose, lactose, maltose, mannitol, sucrose). In conclusion, <em>P. africana</em> SUG3.1 and <em>C. micaceus</em> SUG4.3 may complement <em>L. plantarum</em> as biocontrol agents against post-harvest pathogens of tomatoes.</p></div>","PeriodicalId":12683,"journal":{"name":"Fungal biology","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1878614624000655/pdfft?md5=439f87954371e4f977f0e19666efac2b&pid=1-s2.0-S1878614624000655-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141089820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Endophytic fungal isolates from apple tissue: Latent pathogens lurking within?","authors":"Lay Lay Nwe,&nbsp;Seona Casonato,&nbsp;E. Eirian Jones","doi":"10.1016/j.funbio.2024.05.003","DOIUrl":"https://doi.org/10.1016/j.funbio.2024.05.003","url":null,"abstract":"<div><p>Fungal endophytes inhabit a similar ecological niche to that occupied by many phytopathogens, with several pathogens isolated from healthy tissues in their latent phase. This study aimed to evaluate the pathogenicity, the colonisation ability, and the enzyme activity of 37 endophytic fungal isolates recovered from apparently healthy apple shoot and leaf tissues. The pathogenicity of the isolates was assessed on ‘Royal Gala’ and ‘Braeburn’ fruit and detached ‘Royal Gala’ shoots. For the non-pathogenic isolates, their ability to endophytically colonise detached ‘Royal Gala’ shoots was evaluated. Enzyme activity assays were undertaken to determine whether the pathogenicity of the endophytes was related to the production of the extracellular enzymes, amylase, cellulase, pectinase, protease, and xylanase. Of the 37 isolates studied, eight isolates, representing the genera <em>Colletotrichum</em>, <em>Diaporthe</em>, <em>Fusarium,</em> and <em>Penicillium,</em> were shown to be pathogenic on both apple shoots and fruit. Two isolates identified as <em>Trichoderma atroviride</em>, were pathogenic only on shoots, and three isolates, representing the genus <em>Diaporthe</em>, were pathogenic only on fruit. Of the remaining 24 isolates, 22 (<em>Biscogniauxia</em> (n = 8), <em>Chaetomium</em> (n = 4), <em>Trichoderma</em> (n = 3), <em>Epicoccum</em> (n = 2), <em>Neosetophoma</em> (n = 2), <em>Xylaria</em> (n = 1), <em>Daldinia</em> (n = 1), and <em>Paraphaeosphaeria</em> (n = 1)) were recovered from the inoculated apple shoots but two failed to colonise the shoot tissues. Of the isolates tested, 20 produced amylase, 15 cellulase, 25 pectinase, 26 protease, and 13 xylanase. There was no correlation between the range and type of enzymes produced by the isolates and their pathogenicity or ability to endophytically colonise the shoot tissue. The study showed that approximately one-third (13/37) of the isolates recovered from the apparently healthy apple shoot tissues were observed as latent pathogens. The isolates that did not cause disease symptoms may have the ability to reduce colonisation of apple tissues by pathogens including <em>Neonectria ditissima</em> associated with European canker of apple.</p></div>","PeriodicalId":12683,"journal":{"name":"Fungal biology","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1878614624000588/pdfft?md5=41e5446519faf7b54c0ce16522474a55&pid=1-s2.0-S1878614624000588-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141073292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physiological response of Metarhizium rileyi with linoleic acid supplementation","authors":"Leidy Esther Sánchez-Rey ,&nbsp;Nubia Moreno-Sarmiento ,&nbsp;Erika Paola Grijalba-Bernal ,&nbsp;Ginna Quiroga-Cubides","doi":"10.1016/j.funbio.2024.05.002","DOIUrl":"10.1016/j.funbio.2024.05.002","url":null,"abstract":"<div><p><em>Metarhizium rileyi</em> has a broad biocontrol spectrum but is highly sensitive to abiotic factors. A Colombian isolate <em>M. rileyi</em> Nm017 has shown notorious potential against <em>Helicoverpa zea.</em> However, it has a loss of up to 22 % of its conidial germination after drying, which limits its potential as a biocontrol agent and further commercialization. Conidial desiccation resistance can be enhanced by nutritional supplements, which promotes field adaptability and facilitates technological development as a biopesticide. In this study, the effect of culture medium supplemented with linoleic acid on desiccation tolerance in Nm017 conidia was evaluated. Results showed that using a 2 % linoleic acid-supplemented medium increased the relative germination after drying by 41 % compared to the control treatment, without affecting insecticidal activity on <em>H. zea</em>. Also, the fungus increased the synthesis of trehalose, glucose, and erythritol during drying, independently of linoleic acid use. Ultrastructural analyses of the cell wall-membrane showed a loss of thickness by 22 % and 25 %, in samples obtained from 2 % linoleic acid supplementation and the control, respectively. Regarding its morphological characteristics, conidia inner area from both treatments did not change after drying. However, conidia from the control had a 24 % decrease in length/width ratio, whereas there was no alteration in conidia from acid linoleic. The average value of dry conidia elasticity coefficient from linoleic acid treatment was 200 % above the control. Medium supplementation with linoleic acid is a promising fermentation strategy for obtaining more tolerant conidia without affecting production and biocontrol parameters, compatible solutes synthesis, or modifying its cell configuration.</p></div>","PeriodicalId":12683,"journal":{"name":"Fungal biology","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141056452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spatial distribution and community composition of endophytic fungi within Mussaenda pubescens stems","authors":"Meijuan Xi ,&nbsp;Yonglong Wang ,&nbsp;Juanjuan Yang ,&nbsp;Xiaohui Bi ,&nbsp;Shengen Zhong ,&nbsp;Tingting Duan ,&nbsp;Yimin He ,&nbsp;Tieyao Tu ,&nbsp;Xin Qian","doi":"10.1016/j.funbio.2024.05.001","DOIUrl":"10.1016/j.funbio.2024.05.001","url":null,"abstract":"<div><p>Endophytic fungi, pivotal in facilitating plant co-evolution, significantly enhance plant growth, stress resistance, and environmental adaptability. Despite their importance, the spatial distribution of stem endophytic fungi (SEF) within host plants remains poorly characterized. Here, we employed high-throughput sequencing to conduct a comparative analysis of SEF communities in <em>Mussaenda pubescens</em> on a regional scale. Our findings reveal that whole-SEF communities were overwhelmingly dominated by members of the phylum <em>Ascomycota</em>, accounting for 85.9 %, followed by <em>Basidiomycota</em> at 13.9 %, and that alpha diversity within the whole-SEF community of <em>M</em>. <em>pubescens</em> remains relatively consistent across sampling sites. However, significant variation was observed within conditionally abundant taxa (CAT), conditionally rare or abundant taxa (CRAT), and conditionally rare taxa (CRT). Climatic factors emerged as the primary influence on SEF community distribution, followed by spatial distance and stem chemical properties. Neutral community modeling results suggested that both stochastic and deterministic processes play a role in shaping whole-SEF communities, with deterministic processes having a stronger influence on CRT subcommunities. Furthermore, the CRT co-occurrence network exhibited a more complex structure, characterized by higher values of network betweenness and degree relative to CAT and CRAT subcommunities. These findings enhance our understanding of community assembly and ecological interactions between stem fungal endophytes, presenting opportunities for harnessing fungal resources for the benefit of humanity.</p></div>","PeriodicalId":12683,"journal":{"name":"Fungal biology","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141062105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}