Gene Reports最新文献

{"title":"Genetical and cellular induction of interferon genes via the treatment with (Allium sativum) garlic extract against recombinant influenza A/Puerto Rico/8/34 H1N PR8 infection","authors":"Omer Abid Kathum ,&nbsp;Saafa Abd.Al-kahdum ,&nbsp;Umniah Abd-Al-Naser Saleh Hadi ,&nbsp;Sarah Kamil Abood ,&nbsp;Shaimaa Y. Abdulfattah","doi":"10.1016/j.genrep.2024.102069","DOIUrl":"10.1016/j.genrep.2024.102069","url":null,"abstract":"<div><h3>Background</h3><div>It had been established that; influenza A viral infection is connected in a big range with stimulation of many cellular kinases which either being necessary for viral live cycle or as a kind of antagonistic response against this attack hoping to stop the viral invasion. This induction includes many cellular mediator's response. Among them; induction of Retinoic Inducible Genes I (RIG I) which is classified as the precursor of interferon β activation thereby the activation of another mediator like IRF3.</div><div>The objective of this study is to identify and establish a new and natural antiviral active compound which might help in enhancing the cellular immunity against influenza A virus thereby attenuating its ability to invade living cells.</div></div><div><h3>Methods</h3><div>In this work, variety of concentration of Alluim sativum (AS) or garlic plant extract; 12.5, 25, 50 μg/ml were tested on MDCKII or A549 cell as an in vitro experimental work to examine its ability to abort the replication of Influenza A/Puerto Rico/8/34 H1N1 (IAV PR8) strain depending on many techniques like, viral plaque assay, gene expression by real time PCR (rt PCR), luciferase assay and immunofluorescent stating.</div></div><div><h3>Results</h3><div>Our data explained that there was weak replication ability as explained in viral replication titer by plaque assay whether after 8 hours post infection using 0.1 MOI or even after 24 hours post infection using 0.01 MOI of influenza A PR8, this finding was propped with a prosaic expression of some viral protein genes like NS1, NP. the immunofluorescent staining also supported those data via the weak localization of NS1 protein during the treatment with garlic extract and that’s normally is connected with high induction of cellular gene expression representing by RIG I, IRF3 mediator and Interferon β genes necessary for induction of type I interferon caused by treatment with garlic extract.</div></div><div><h3>Conclusion</h3><div>Allium sativum plant extract sequestered influenza A virus PR8 replication significantly especially, at concentration of 50 μg/ ml which is the best concentration that can act against the virus due to acute induction of cellular proteins represented by RIG I pathogen recognizer and other proteins that interferes with activation of interferon then preventing the viral competition with cellular immunity to invade the cells.</div><div>In another word, the garlic extract is a direct inducer for RIG I and other mediators important for activation of type I interferon pathway as an immune response to prevent viral attack.</div></div>","PeriodicalId":12673,"journal":{"name":"Gene Reports","volume":"37 ","pages":"Article 102069"},"PeriodicalIF":1.0,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142587269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of sex chromosome abnormalities in male chronic myeloid leukemia patients","authors":"Mahitha Mohanan Sreelatha ,&nbsp;Narayanan Geetha ,&nbsp;Vineetha Radhakrishnan Chandraprabha ,&nbsp;Preethi Gopinath ,&nbsp;Akhila Raj Thampirajan Vimala Devi ,&nbsp;Geetha Raj John Anitha ,&nbsp;Amritha Padmakumar ,&nbsp;Devipriya Padmakumar ,&nbsp;Hariharan Sreedharan","doi":"10.1016/j.genrep.2024.102066","DOIUrl":"10.1016/j.genrep.2024.102066","url":null,"abstract":"<div><h3>Introduction</h3><div>Chronic Myeloid Leukemia (CML) is a myeloproliferative disorder characterized by the Philadelphia chromosome (Ph) resulting from the reciprocal translocation t(9;22)(q34;q11). Sex chromosomal abnormalities are found rarely in CML patients.</div></div><div><h3>Materials and methods</h3><div>Conventional and molecular cytogenetic analysis (Fluorescent <em>In Situ</em> Hybridization (FISH)) were performed to reveal the presence of Ph and additional chromosomal abnormalities (ACA).</div></div><div><h3>Results</h3><div>Three patients with abnormalities within sex chromosomes, one Klinefelter syndrome (KS) patient and two cases with LOY (Loss of Y) diagnosed with CML CP were included in the study. All three patients initially responded to the targeted therapy, became resistant to their treatment course and switched to 2nd line therapies. In the 15th month, the KS patient abruptly turned into BC and showed clonal evolution with four karyotypic patterns. According to Mitelman databases, this is the first KS-CML patient who showed three major route abnormalities, +8, i(17) and + der(22) in a single clone. Survival analysis showed one patient with LOY chromosome expired on the 48th month of diagnosis.</div></div><div><h3>Conclusion</h3><div>The application of cytogenetic techniques in identifying the constitutional abnormality of KS, ACA at initial CML diagnosis, revealing of extra Ph during the treatment course and clonal evolution in BC (Blast Crisis) assist the proper monitoring of disease transformation and serves as a major diagnostic tool for CML patients thereby switching the treatment protocols. A systematic stratification of patients according to the chromosomal abnormalities is needed in CML patients.</div></div>","PeriodicalId":12673,"journal":{"name":"Gene Reports","volume":"37 ","pages":"Article 102066"},"PeriodicalIF":1.0,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142573478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intra-segmental recombinations between avian and mammalian VP4 genotypes in Rotavirus alphagastroenteritidis","authors":"Yoshiyuki Suzuki,&nbsp;Masaya Yaeshiro,&nbsp;Daiki Uehara","doi":"10.1016/j.genrep.2024.102063","DOIUrl":"10.1016/j.genrep.2024.102063","url":null,"abstract":"<div><div><em>Rotavirus alphagastroenteritidis</em> (RVA), a pathogen causing acute gastroenteritis in young birds and mammals, possesses a genome of 11 segmented double-stranded RNAs, each of which is classified into genotypes. RVA has been divided into the avian and mammalian clusters as well as the basal group. Although the avian and mammalian clusters were considered to have evolved independently, genomic segment encoding viral protein 4 (VP4) of pheasant strain with genotype P[37] appeared to be transferred from mammalian cluster strain through reassortment. Here prototype sequences for all genotypes P[1]-P[58] of VP4 segment except for P[53] and P[54] retrieved from the International Nucleotide Sequence Database were analyzed to examine the possibility that P[37] was a product of intra-segmental recombinations between avian and mammalian cluster strains. In the sliding window analysis, different regions of P[37] appeared to have different relative similarities to avian and mammalian cluster strains. Using recombination detection programs, two regions of P[37] were identified to be derived from mammalian cluster strains and integrated into the background of avian cluster strain. These results were confirmed by phylogenetic analyses. Thus, even when genomic segments are not entirely compatible between strains, only compatible parts may be transferred through intra-segmental recombinations in RVA.</div></div>","PeriodicalId":12673,"journal":{"name":"Gene Reports","volume":"37 ","pages":"Article 102063"},"PeriodicalIF":1.0,"publicationDate":"2024-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142537373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extended-spectrum β-lactamase variants in Escherichia coli, Klebsiella pneumoniae and Proteus mirabilis from community- and hospital-acquired urinary tract infections","authors":"Lubna Razaq ,&nbsp;Fakhur Uddin ,&nbsp;Sanum Ali ,&nbsp;Shahzad Ali ,&nbsp;Rizwana Kausar ,&nbsp;Muhammad Sohail","doi":"10.1016/j.genrep.2024.102065","DOIUrl":"10.1016/j.genrep.2024.102065","url":null,"abstract":"<div><div>Extended-spectrum-β-lactamase (ESBL)-producing Enterobacterales, especially <em>Escherichia coli</em>, <em>Klebsiella pneumoniae</em>, and <em>Proteus mirabilis</em>, are highly prevalent in various parts of the world. However, the prevalence of ESBL variants in Enterobacterales from hospital-acquired (HA) and community-acquired (CA) urinary tract infections (UTI) had been infrequently reported in developing countries. This study analyzed the presence of ESBL-producing Enterobacterales, their resistance patterns, and ESBL variants. Of the 279 urine samples, 144 (51.6 %) were obtained from patients with CA-UTI and 135 (48.4 %) were obtained from patients with HA-UTI. From these samples, 145 and 135 Enterobacterales strains were isolated from the CA and HA-UTI groups, respectively. <em>E. coli</em> was the predominant (202/280; 72.2 %), followed by <em>K. pneumoniae</em> (53/280; 18.9 %) and <em>P. mirabilis</em> (25/280; 8.9 %). ESBL producers were higher (131/218; 60.09 %) in the HA-UTI isolates. Overall, <em>bla</em>_SHV (48.2 %) was the predominant gene, followed by <em>bla</em>_TEM (44 %), whereas <em>bla</em>_CTX-M was rarely detected (7.79 %) in the CA and HA-UTI isolates. <em>bla</em>_SHV-212, <em>bla</em>_SHV-229, <em>bla</em>_TEM-₁₀₃, and <em>bla</em>_TEM-₁₀₄ were common among the isolates. The coexistence of <em>bla</em>_TEM and <em>bla</em>_<em>S</em>HV was also observed. This study highlights a different ESBL pattern among uropathogens that can aid in updating infectious disease control and antimicrobial stewardship guidelines.</div></div>","PeriodicalId":12673,"journal":{"name":"Gene Reports","volume":"37 ","pages":"Article 102065"},"PeriodicalIF":1.0,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142537374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Azoospermia factor gene microdeletions in infertile men with non-obstructive azoospermia and normal karyotype: First case-control study from Kashmir","authors":"Faisel Ahmad ,&nbsp;Mahrukh Hameed Zargar ,&nbsp;Mohammad Lateef ,&nbsp;Arshad Hussain ,&nbsp;Tahir Mohuiddin Malla ,&nbsp;Mohd Ashraf Ganie ,&nbsp;Iqbal Qasim ,&nbsp;Sajad Ul Islam Mir ,&nbsp;Saima Wani ,&nbsp;Nadia Khurshid","doi":"10.1016/j.genrep.2024.102064","DOIUrl":"10.1016/j.genrep.2024.102064","url":null,"abstract":"&lt;div&gt;&lt;h3&gt;Background&lt;/h3&gt;&lt;div&gt;Micro-deletions in the Y chromosome are recognized as the causative factor for male infertility. The prevalence of Y chromosome micro-deletions exhibits variation among infertile males across different areas and races globally. The study of Y chromosome micro- deletions is crucial among genetic variables owing to their ability to transmit genetic defects to the progeny. Microdeletion of the azoospermia factor (AZF) region associated with the long arm of the Y chromosome (Yq) has three sub-regions (AZFa, AZFb, and AZFc) that play an important role in spermatogenesis. The genes associated with the AZF region of the Y chromosome are believed to play a crucial role in the process of spermatogenesis by performing several activities such as gene silencing, transcription, ubiquitination, and maintenance of microtubule networks. Due to the absence of epidemiological research on Y chromosome micro-deletions in ethnic infertile male population of Kashmir, our study sought to examine the Y chromosome micro-deletions among non-obstructive azospermic infertile men in Kashmir.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Objective&lt;/h3&gt;&lt;div&gt;The research was aimed to establish the frequency and characteristics of micro-deletions in the AZF region of Y chromosome in infertile males of our population with non-obstructive Azoospermia and normal Karyotype.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Methods&lt;/h3&gt;&lt;div&gt;A total of 120 subjects were included in the study. Samples from 60 male patients with fertility issues (non-obstructive azoospermia) and an equal number of samples from normal men having established fatherhood (biological fathers) were taken for the study. The average age in years of cases and controls were 32.80 and 34.88 respectively. A total of 26.66 % of cases and 13.33 % of controls were found to be consanguineous, 36.66 % of cases and 40 % of controls were urban while 63.33 % of cases and 60 % of controls were from rural population. Molecular analysis was performed by multiplex polymerase chain reactions (PCR) using sequence tagged sites (STS) from 3 different regions of AZF of Y chromosome. To assess the frequency of AZF micro-deletions, molecular analysis was performed by multiplex polymerase chain reactions (PCR) using sequence tagged sites (STS) from 3 different regions of the Y chromosome (sY84 and sY86 for AZFa region; sY127 and sY134 for AZFb; sY254 and sY255 for AZFc region).&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Results&lt;/h3&gt;&lt;div&gt;In the present study a total of 9 out of 60 cases (15 %) were found to have Y chromosome micro- deletions in AZF region of Yq arm. The most frequent micro deletions were observed in AZF&lt;sub&gt;b&lt;/sub&gt; region, 8 out of 60 cases (13.33 %) from AZF&lt;sub&gt;b&lt;/sub&gt; region were found to have deletions.5 out of 60 cases (8.33 %) were reported with deletions associated to AZFc region region and 6.66 % of cases were found to harbor deletions in both AZF&lt;sub&gt;b&lt;/sub&gt; and AZF&lt;sub&gt;c&lt;/sub&gt; region. However no deletion was reported in the AZFa region in all the studied cas","PeriodicalId":12673,"journal":{"name":"Gene Reports","volume":"37 ","pages":"Article 102064"},"PeriodicalIF":1.0,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142537372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A comprehensive study of highly repetitive WD40 proteins in cyanobacteria","authors":"Krishna Kumar Rai","doi":"10.1016/j.genrep.2024.102057","DOIUrl":"10.1016/j.genrep.2024.102057","url":null,"abstract":"<div><div>The WD40 repeat-containing proteins are ancient proteins regulating various cellular and biological processes in eukaryotes. WD40 proteins are extensively studied in eukaryotes, and their genome-wide characterisation has revealed their hidden potential. On the contrary, in-depth taxonomic and functional description of WD40 proteins in prokaryotes, particularly in cyanobacteria, remains largely unexplored. In this study, we have comprehensively analysed cyanobacterial WD40 proteins and detailed comparisons among different cyanobacterial orders. About 7000 WD40 proteins were identified in all six cyanobacterial orders accounting for 22–43 % of all WD40s. While their abundance was less in Chroococcales, Pleurocapsales and, Stigonematales, the WD40s were profoundly present in Nostcales and Oscillatoriales, exhibiting multifarious functions such as cell signalling, transcription factors, catalytic enzymes and scaffold etc. Current systemic analysis showed that most WD40 proteins contain multiple WD40 domains, as indicated by their repeat numbers and average confidence scores. The observation also indicated that most WD40 proteins have complex hydrogen bond networks. Their taxonomic distribution and gene neighbourhood analysis revealed topical or newly repeated duplication events form most WD40s. Further, the studies confirmed that the recently formed WD40 proteins are highly repetitive with higher structural stability. Overall, the result of this study has described an assembly of cyanobacterial WD40 proteins and highlighting their evolution, distribution and probable functions.</div></div>","PeriodicalId":12673,"journal":{"name":"Gene Reports","volume":"37 ","pages":"Article 102057"},"PeriodicalIF":1.0,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142440981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolic changes in response to food intake in somatostatin 1.1 deficient zebrafish","authors":"Jie Chen ,&nbsp;Huiming Yuan ,&nbsp;Jing Gao ,&nbsp;Lu Liu ,&nbsp;Adelino V.M. Canario","doi":"10.1016/j.genrep.2024.102062","DOIUrl":"10.1016/j.genrep.2024.102062","url":null,"abstract":"<div><div>Somatostatin is a multifunctional hormone with several genes in teleost fishes. A zebrafish CRISPR/Cas9 knockout of the <em>somatostatin 1.1</em> (<em>sst1.1</em>) with persistent hyperglycaemia and hyperlipidaemia displayed reduced fecundity when fed brine shrimp ad libitum. Here, we investigated the effect of feeding brine shrimp one to three times a day on fecundity and liver transcriptomics of the <em>sst1.1</em> mutant compared to their wild-type siblings to unravel molecular pathways associated with the phenotype. We find that the <em>sst1.1</em> deficient zebrafish had high mortality when fed at the highest rate and that in both genotypes, growth and fecundity were proportional to food intake. Although glucose and cholesterol decreased substantially at the lowest level of feeding, they were still higher in the mutant than in the wild-type zebrafish. Furthermore, <em>sst1.1</em> deficiency had a small but significant effect on the hepatic expression of protein, carbohydrate, and fatty acid biosynthesis genes, contributing to the mutant's diabetic phenotype.</div></div>","PeriodicalId":12673,"journal":{"name":"Gene Reports","volume":"37 ","pages":"Article 102062"},"PeriodicalIF":1.0,"publicationDate":"2024-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142438001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CC chemokine receptor 5 and CC chemokine ligand 5 gene polymorphisms in patients with periodontitis - A case–control study","authors":"Ayshwarya Karthika Muralidharan,&nbsp;Sangeetha Subramanian,&nbsp;Prakash P.S.G.,&nbsp;Devapriya Appukuttan,&nbsp;Jasmine Crena,&nbsp;Anitha C.M.","doi":"10.1016/j.genrep.2024.102061","DOIUrl":"10.1016/j.genrep.2024.102061","url":null,"abstract":"<div><h3>Aim</h3><div>This study aimed to evaluate the association of CC Chemokine Receptor 5 (CCR5) G59029A and CC Chemokine Ligand 5 (CCL5) -28 C/G gene polymorphisms in patients with and without periodontitis.</div></div><div><h3>Materials and methods</h3><div>A total of 172 individuals were enrolled, divided into two groups: Group I (periodontally healthy, n = 86) and Group II (generalized chronic periodontitis, n = 86). Periodontal clinical parameters such as Periodontal Probing Depth (PPD), Clinical Attachment Loss (CAL), Plaque Index (PI), and Bleeding Index (BI) were recorded. Allele-specific PCR (AS-PCR) was used to identify polymorphic sites in the CCL5 and CCR5 genes.</div></div><div><h3>Results</h3><div>The heterozygous genotype CG and allele G was more prevalent in the Test group (p value = 0.001, 0.01) for CCL5 gene polymorphism. Similarly, the heterozygous genotype AG and allele G for CCR5 gene polymorphism was significantly higher in the Test group (p value = 0.002, 0.04).</div></div><div><h3>Conclusion</h3><div>The study found a significant association between CCL5 and CCR5 gene polymorphisms and periodontitis.</div></div>","PeriodicalId":12673,"journal":{"name":"Gene Reports","volume":"37 ","pages":"Article 102061"},"PeriodicalIF":1.0,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142425212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Drug resistance of urease-positive bacteria other than Helicobacter pylori and distribution of urease genes in patients with gastritis","authors":"Elham Amiri ,&nbsp;Hamid Reza Goli ,&nbsp;Mehrdad Gholami ,&nbsp;Zohre Bari ,&nbsp;Arash Kazemi Veisari ,&nbsp;Hafez Tirgar Fakheri ,&nbsp;Jamshid Yazdani Charati ,&nbsp;Maryam Salehiyan ,&nbsp;Mohammad Ahanjan","doi":"10.1016/j.genrep.2024.102058","DOIUrl":"10.1016/j.genrep.2024.102058","url":null,"abstract":"<div><div>Urease-positive bacteria other than <em>Helicobacter pylori</em> have been shown to be present in the mouth, stomach, intestines, urinary tract, and skin. The aim of this study was to evaluate the prevalence of non-<em>H. pylori</em> urease-positive bacteria in the gastric biopsies of patients with gastritis and the antibiotic resistance pattern of the isolates, along with the prevalence of <em>ureA</em>, <em>ureB</em>, and <em>ureC</em> genes. In this study, 165 biopsies were collected from the gastric antrum of patients with gastritis referred to hospitals by a gastroenterologist. After Rapid Urease Test, the samples were transferred to the microbiology laboratory using a Brain Heart Infusion broth transfer medium. Next, the non-<em>H. pylori</em> bacteria were identified by the standard microbiological methods. Also, the <em>H. pylori</em>-positive samples were detected using the pathological testing, stool antigen detection test, and enzyme linked sorbent assay. However, after the growth and purification of microorganisms, the urease test was carried out again. In the next step, the DNAs of all confirmed isolates were extracted and the presence of <em>ureA</em>, <em>ureB</em>, and <em>ureC</em> genes was evaluated using the specific primers by the PCR method. Among the 100 urease-positive biopsies, 77 samples were infected with <em>H. pylori</em> and 23 were non-<em>H. pylori</em>-positive. <em>Staphylococcus epidermidis</em> was the most prevalent non-<em>H. pylori</em>-positive bacteria in this study. The antibiotic resistance pattern of the bacteria showed that tetracycline and erythromycin were the least effective antibiotics against the gram-positive and -negative, respectively. However, the lowest resistance rate of gram-positive bacteria was detected against co-trimoxazole, while cefotaxime, chloramphenicol, and ceftriaxone were the most effective antibiotics against the gram-negative bacteria. In addition, the <em>ureA</em> gene was detected among 21.73 % of the non-<em>H. pylori</em> isolates, while 8.69 % and 43.47 % of them were <em>ureB</em> and <em>ureC</em> positive, respectively. This study showed a considerable significance of non-<em>H. pylori</em> urease-positive bacteria causing gastritis. It seems that the diagnosis of these organisms can be effective in treatment of patients with gastritis. Also, the <em>ureC</em> gene was predominant to produce the urease in these isolates.</div></div>","PeriodicalId":12673,"journal":{"name":"Gene Reports","volume":"37 ","pages":"Article 102058"},"PeriodicalIF":1.0,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142438000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"FDXR gene expression and micronucleus frequency in Type 2 diabetes mellitus patients and their importance in case of radiation exposures","authors":"Shravani A S ,&nbsp;Priyanka R ,&nbsp;Indumathi A N ,&nbsp;Prabhakar Kamarthy ,&nbsp;Venkatachalam Perumal ,&nbsp;Venkateswarlu Raavi","doi":"10.1016/j.genrep.2024.102060","DOIUrl":"10.1016/j.genrep.2024.102060","url":null,"abstract":"<div><div>Analysis of gene expression (e.g. Ferredoxin Reductase: <em>FDXR</em>) changes in the blood samples have shown potential as predictive markers of disease, prognosis for therapy as well as triage and dose estimation in radiation-exposed populations. Similarly, quantification of micronuclei (MN) formation has been presented as a rapid cytogenetic marker for those applications. It was cautioned that the reliable utilization of these markers for prediction of disease, therapy prognosis, and dose estimation depends upon the information on known variables that affect these markers. Literature suggests that advanced glycation end products/oxidative stress in diabetic conditions can alter the levels of DNA damage and gene expression, and impact the segregation of the exposed from unexposed during nuclear disasters. Therefore, we investigated the influence of Type 2 diabetes mellitus (T2DM) on baseline expression of the <em>FDXR</em> and frequency of MN. Peripheral blood samples were collected from healthy volunteers (HV) (<em>n</em> = 60; 43 males and 17 females) and T2DM patients (n = 60; 32 males and 28 females), and performed real-time quantification of <em>FDXR</em> gene expression and analysis of MN frequency using microscopy. The basal level of <em>FDXR</em> gene expression (2.55 folds) (<em>p</em> &lt; 0.01) and the frequency of MN is significantly (<em>p</em> &lt; 0.01) higher (4 folds) in T2DM patients when compared to HV. Further, subgroup analysis found that gender, alcohol, smoking, duration of T2DM, complications, and medications increased both the expression of the <em>FDXR</em> gene and frequency of MN in T2DM; nevertheless, the increase was not significant, except for gender (<em>p</em> &lt; 0.05) and medication (<em>p</em> &lt; 0.05) on the frequency of MN. Overall results indicate that the T2DM patients showed a higher basal level expression of <em>the FDXR</em> gene and MN frequency when compared to HV and suggest an altered metabolic condition in T2DM is a confounding factor that impacts the levels of those markers. The increased levels of these markers might need to be considered to monitor medical radiation exposures and reliable biodosimetry during large-scale radiological accidents.</div></div>","PeriodicalId":12673,"journal":{"name":"Gene Reports","volume":"37 ","pages":"Article 102060"},"PeriodicalIF":1.0,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142425213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}