Gene Reports最新文献

{"title":"The prevalence of chemokine receptors (CCR5-Δ32, CCR5-m303, CCR2-64I, and SDF1-3'A) in the population with sickle cell anemia","authors":"Motiram Sahu ,&nbsp;Dinesh Kumar ,&nbsp;Somendra Kumar ,&nbsp;Anil Kumar","doi":"10.1016/j.genrep.2025.102261","DOIUrl":"10.1016/j.genrep.2025.102261","url":null,"abstract":"<div><h3>Objective</h3><div>The objective of the present research was to examine the presence of gene polymorphisms within chemokine receptors in a population with Sickle Cell Disease (SCD) and in a group of individuals without health complications. The selected genotypes were the chemokine receptor genes CCR5-Δ32, CCR5-m303, CCR2-64I, and SDF1-3'A.</div></div><div><h3>Methods</h3><div>From Chhattisgarh, India, 2586 individuals were screened for sickle cell anemia, out of which 200 individuals with SCD and 50 as controls were subjected to genotyping of CCR5-Δ32, CCR5-m303, CCR2-64I, and SDF1-3'A gene polymorphisms using RT-qPCR, followed by RFLP. The frequencies of both genotypes and alleles were calculated, and the assessment of Hardy-Weinberg equilibrium was performed using the chi-squared goodness-of-fit test.</div></div><div><h3>Results</h3><div>We report a 10.55 % (HbAs-9.78 % &amp; HbSS-0.77 %) prevalence of sickle cell anemia in Chhattisgarh, India, and the allelic frequencies of CCR5/CCR5 and Δ32/Δ32 as 0.966 and 0.034, respectively (χ2 = 0.3097, P-0.85654). The CCR5-m303 mutation was not detected in any of the individuals, indicating the absence of mutant alleles. The allelic frequencies for the wild-type CCR2-64 V allele and the CCR2-64I variant were calculated at 0.84 and 0.16 (χ2 = 6.9444, <em>P</em> = 0.3105) and the wild-type SDF1 and the 3′A variant were determined as 0.732 and 0.268, respectively (χ2 = 0.9630, P-061783).</div></div><div><h3>Conclusions</h3><div>The present study provides essential insights into the prevalence of chemokine receptor gene polymorphisms in SCD and healthy populations. The findings indicate distinct patterns in the distribution of genotypes and alleles, consistent with Hardy-Weinberg equilibrium. The variant CCR5-Δ32 is found to be helpful in inhibiting inflammation, especially in the SCD population.</div></div>","PeriodicalId":12673,"journal":{"name":"Gene Reports","volume":"40 ","pages":"Article 102261"},"PeriodicalIF":1.0,"publicationDate":"2025-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144072562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrative computational analysis of HCMV-encoded miRNAs: Mapping host protein interactions and immune evasion mechanisms","authors":"Aroni Chatterjee ,&nbsp;Shreya Dey ,&nbsp;Hiya Ghosh ,&nbsp;Sanjukta Dasgupta","doi":"10.1016/j.genrep.2025.102255","DOIUrl":"10.1016/j.genrep.2025.102255","url":null,"abstract":"<div><div>Human Cytomegalovirus (HCMV) is a widespread virus that poses serious risks to newborns and individuals with weakened immune systems, often causing severe disease. One of the virus's most cunning strategies for survival is its ability to produce its microRNAs (miRNAs), which allow it to subtly hijack the host's cellular machinery, evade immune detection, and persist in the body. The interaction between miRNAs and their target proteins in host cells provides significant insights into the mechanisms of HCMV infection. In recent years, computational tools have become essential in uncovering the secrets of these viral miRNAs. Using a combination of machine learning models, sequence alignment tools, and secondary structure prediction algorithms, researchers have been able to identify HCMV-encoded miRNAs and predict their interactions with host and viral genes. These in silico approaches help trace how viral miRNAs interfere with key immune processes, such as antigen presentation and interferon signaling, offering insight into how HCMV weakens antiviral responses. Computational analyses also reveal how these miRNAs affect protein-protein interactions and cellular pathways crucial for immune defense. Despite these advances, challenges remain, including difficulties in validating predictions and understanding the context-dependent roles of miRNAs. This review highlights how computational biology has transformed our understanding of HCMV-host interactions, while also acknowledging the need for more accurate, integrative models to bridge the gap between prediction and biological reality.</div></div>","PeriodicalId":12673,"journal":{"name":"Gene Reports","volume":"40 ","pages":"Article 102255"},"PeriodicalIF":1.0,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144070056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Non-invasive saliva-based profiling of TP53 codon 72 polymorphism in oral cancer patients by droplet digital PCR","authors":"Rhea Kishore ,&nbsp;Sonam N. Kille ,&nbsp;Reena Kishore ,&nbsp;Gunimala Chakraborty ,&nbsp;Vinay Kumar J. Rajendra ,&nbsp;Audrey D'Cruz ,&nbsp;Anirban Chakraborty","doi":"10.1016/j.genrep.2025.102256","DOIUrl":"10.1016/j.genrep.2025.102256","url":null,"abstract":"<div><div>Germline polymorphisms in tumor suppressor genes are linked to predisposition to several types of cancers. <em>TP53</em> is one of the most frequently mutated genes in oral cancer and several polymorphisms have been reported in this important tumor suppressor gene. The c.215C &gt; G (p.Pro72Arg) polymorphism in exon 4 of <em>TP53</em> is a well-studied polymorphism in oral cancer. In this study, the status of c.215C &gt; G polymorphism was checked in saliva of 33 oral cancer patients by a liquid biopsy approach using the ultrasensitive Droplet Digital PCR (ddPCR) technology. The results revealed that the C/G genotype (Pro/Arg) was present in 45.5 % of the samples whereas 36.3 % and 18.2 % samples showed the presence of G/G and C/C alleles respectively. The results obtained from the saliva-derived cfDNA were in concordance with those obtained using tumor tissue-derived DNA. The study demonstrates the use of ddPCR as a non-invasive method for saliva-based genotyping of <em>TP53</em> polymorphisms in oral cancer.</div></div>","PeriodicalId":12673,"journal":{"name":"Gene Reports","volume":"40 ","pages":"Article 102256"},"PeriodicalIF":1.0,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144069433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigating the association of FSHB -211G > T polymorphism with male infertility and reproductive parameters in the men from South-West of Iran","authors":"Zahra Zanganehnejad ,&nbsp;Alihossein Saberi ,&nbsp;Milad Pezeshki ,&nbsp;Zohre Zanganeh Fard","doi":"10.1016/j.genrep.2025.102254","DOIUrl":"10.1016/j.genrep.2025.102254","url":null,"abstract":"&lt;div&gt;&lt;h3&gt;Background&lt;/h3&gt;&lt;div&gt;Male infertility is a complex disorder that arises from a combination of genetic and environmental factors. The presence of a particular genetic variation, referred to as rs10835638 (c.-211G &gt; T) polymorphism in the follicle stimulating hormone beta subunit (FSHB) gene, might be involved in the disruption of reproductive parameters and its association with male infertility. The aim of this research is to examine the correlation between the FSHB -211G &gt; T polymorphism and the susceptibility to male infertility in South-west of Iran.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Methods&lt;/h3&gt;&lt;div&gt;The study recruited a cohort of 120 patients diagnosed with male infertility, and 120 fertile men serving as a healthy control group, January 2018 until April 2021. By employing the PCR-RFLP technique, the association between &lt;em&gt;FSHB&lt;/em&gt; -211G &gt; T polymorphism with the risk of male infertility was investigated. Additionally, bioinformatics analysis was utilized to further investigate the potential implications of these genetic variations on male reproductive health.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Results&lt;/h3&gt;&lt;div&gt;The genotype and allele frequency analysis for FSHB -211G &gt; T polymorphism show that frequency of GT genotype in the case and control groups, was 22.5 % and 9 %, respectively, also the frequency of TT genotype in the cases is 12 times more than controls group. There was a significant statistical difference between GT (&lt;em&gt;P&lt;/em&gt; = 0.008, OR = 4.682, CI = 95 %, 2.299–8.555) and TT (&lt;em&gt;P&lt;/em&gt; = 0.000, OR = 16.500, CI = 95 %, 4.239–35.510) genotypes and also, T allele (P = 0.000, OR = 5.618, CI = 95 %, 2.848–11.082) of this polymorphism with risk of male infertility. The analysis of association between the &lt;em&gt;FSHB&lt;/em&gt; -211G &gt; T with reproductive parameters in infertile men group revealed the strongest associations to FSH levels. Significantly lower FSH levels were detected in the TT genotype carriers (&lt;em&gt;P&lt;/em&gt; = 0.003, OR = 5.214, CI = 95 %, 1.260–10.317) and also T allele (&lt;em&gt;P&lt;/em&gt; = 0.007, OR = 2.602, CI = 95 %, 1.303–5.194) of &lt;em&gt;FSHB&lt;/em&gt; -211G &gt; T. In contrast the TT genotype (&lt;em&gt;P&lt;/em&gt; = 0.023, OR = 6.250, CI = 95 %, 1.287–12.349) and T allele (&lt;em&gt;P&lt;/em&gt; = 0.001, OR = 3.215, CI = 95 %, 1.610–6.420) of this polymorphism was associated with significantly increased LH level. Also, there was significant statistical difference between the T allele of &lt;em&gt;FSHB&lt;/em&gt; -211G &gt; T polymorphism with decreased sperm motility (&lt;em&gt;P&lt;/em&gt; = 0.009, OR = 2.676, CI = 95 %, 1.868–3.236).&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Conclusion&lt;/h3&gt;&lt;div&gt;The gene polymorphism &lt;em&gt;FSHB&lt;/em&gt; -211G &gt; T has been linked to male infertility. Furthermore, this study has pinpointed a new genetic marker that is connected to decrease FSH levels, elevated LH levels, and reduced sperm motility. This genetic marker shows promise as a molecular diagnostic tool for specific cases of male infertility. Continued research with larger and more diverse populations is essential to full","PeriodicalId":12673,"journal":{"name":"Gene Reports","volume":"40 ","pages":"Article 102254"},"PeriodicalIF":1.0,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144070055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic analysis of selective breeding population and introduced population in Litopenaeus vannamei using SSRs and SNPs","authors":"Chaoan Guo ,&nbsp;Shuo Fu ,&nbsp;Yuan Zhang ,&nbsp;Guangbo Wu ,&nbsp;Xiaoxun Zhou ,&nbsp;Jianyong Liu","doi":"10.1016/j.genrep.2025.102251","DOIUrl":"10.1016/j.genrep.2025.102251","url":null,"abstract":"<div><div>Knowledge of genetic information is crucial for stabilizing diversity and planning selection processes. This study evaluates the genetic diversity of six <em>Litopenaeus vannamei</em> populations (two breeding and four introduced, totaling 180 individuals) using data from 12 microsatellite markers and whole-genome resequencing. After resequencing and filtering, 14,136,203 loci were used for genetic diversity analysis. In SSR analysis, the average observed and expected heterozygosity of the six populations was 0.411 (0.339 to 0.459) and 0.508 (0.402 to 0.558), respectively. Among them, the diversity of SIS in the populations was the highest and XH-F was the lowest. In SNP analysis, the average observed and expected heterozygosity of the six populations was 0.283 (0.252 to 0.312) and 0.292 (0.291 to 0.293), respectively. SIS heterozygosity level was the lowest, but the polymorphism was high, with more low-frequency loci. In population differentiation analysis, the results of the two markers were similar. Both molecular markers were able to classify different populations of <em>L. vannamei</em>. PCA analysis showed that there were obvious differences among different groups. In phylogenetic tree and ancestral estimation analysis, SSR can only classify six populations into three groups. When the K value increases, SSR may face limitations that prevent further subdivision of the population into six groups. In contrast, SNPs exhibit stronger discriminative ability and can clearly divide these six populations into six groups. The results generated from this study will contribute to enriching the genetic resources of <em>L. vannamei</em> and provide important reference information for its artificial breeding and genetic improvement.</div></div>","PeriodicalId":12673,"journal":{"name":"Gene Reports","volume":"40 ","pages":"Article 102251"},"PeriodicalIF":1.0,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143947061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sox9 regulates Papss2 mRNA expression through derepression of the transcriptional repressor, C/EBPβ, in chondrogenic cells","authors":"Cunren Liu,&nbsp;Rosa Serra","doi":"10.1016/j.genrep.2025.102252","DOIUrl":"10.1016/j.genrep.2025.102252","url":null,"abstract":"<div><h3>Objectives</h3><div>Previously, we showed that <em>Papss2</em> expression is regulated by Sox9 in primary chondrocytes and a chondrogenic cell line, ATDC5. Here we explore molecular mechanisms whereby Sox9 regulates mouse <em>Papss2</em> mRNA expression.</div></div><div><h3>Methods</h3><div>Luciferase reporter assays were performed in ATDC5 cells to identify Sox9-responsive elements in the mouse <em>Papss2</em> gene. Electromobility shift assays, mobility shift competition assays, and super shift assays were used to characterize protein binding to a 32 bp Sox9-responsive element. Western blot and co-immunoprecipitation assays were used to determine the effects of Sox9 on C/EBPβ protein levels and binding of Sox9 to C/EBPβ.</div></div><div><h3>Results</h3><div>An evolutionarily conserved 509 bp Sox9-responsive DNA element was identified in the <em>Papss2</em> gene, which was subsequently narrowed down to 32 bp. Putative SoxE and C/EBPβ binding sites were identified within this 32 bp. Increasing amounts of C/EBPβ resulted in attenuation of Sox9-mediated activation of the responsive element indicating C/EBPβ acts as a transcriptional repressor. Three protein-DNA complexes containing C/EBPβ were identified on the Sox9-responsive element under conditions when Papss2 expression was low. With high Sox9 expression, when Papss2 expression was stimulated, the formation of C/EBPβ containing protein-DNA complexes was inhibited, Sox9 and C/EBPβ protein-protein binding was observed, and overall cellular C/EBPβ protein levels were reduced.</div></div><div><h3>Conclusion</h3><div>We propose that Sox9 acts to derepress C/EBPβ-inhibited transcription of Papss2 by first interacting with C/EBPβ to prevent it from binding DNA, then reducing C/EBPβ expression.</div></div>","PeriodicalId":12673,"journal":{"name":"Gene Reports","volume":"40 ","pages":"Article 102252"},"PeriodicalIF":1.0,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143943053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated bioinformatics analysis of shared biomarkers and mechanisms in united airway allergic disease and Alzheimer's disease","authors":"Ken Chen ,&nbsp;Yixing Huang ,&nbsp;Qimei Bao ,&nbsp;Yun Gao ,&nbsp;Xudong Zhao ,&nbsp;Yin Shi ,&nbsp;Xiangdong Cheng ,&nbsp;Zu Ye ,&nbsp;Yaoshu Teng","doi":"10.1016/j.genrep.2025.102244","DOIUrl":"10.1016/j.genrep.2025.102244","url":null,"abstract":"<div><div>Alzheimer's disease (AD) is a leading cause of dementia, whereas united airway allergic disease (UAAD) affects both the upper and lower airways. Epidemiological studies suggest a higher risk of AD in individuals with allergic diseases, hinting at shared molecular mechanisms. This study aims to identify key molecular biomarkers for both diseases and uncover potential mechanisms. Data for AD and UAAD were obtained from the GEO database. Weighted gene co-expression network analysis (WGCNA) identified differentially expressed disease-related genes (DEDRGs). Functional enrichment analysis was subsequently performed for gene functions. Single-sample GSEA (ssGSEA) was used to calculate gene set scores. LASSO regression was used to establish a diagnostic model. Immune infiltration was predicted via the CIBERSORT and ESTIMATE algorithms. Machine learning and protein-protein interactions identified key genes, which were validated through RT-qPCR in airway and brain tissues from mouse models. This study identified <em>GATA2</em>, <em>SLC39A8</em>, and <em>AR</em> as key genes, which were strongly correlated with both diseases. The diagnostic models demonstrated high performance, and RT-qPCR results confirmed elevated co-expression levels of these genes in the disease groups. <em>GATA2</em> has emerged as a crucial gene, involved in immune system activation and the JAK-STAT pathway. This study developed a diagnostic model incorporating <em>GATA2</em>, <em>SLC39A8</em>, and <em>AR</em>, highlighting their role in immune infiltration and disease progression, especially <em>GATA2</em>. These findings suggest a shared mechanism between AD and UAAD, offering new targets for future research and therapy.</div></div>","PeriodicalId":12673,"journal":{"name":"Gene Reports","volume":"40 ","pages":"Article 102244"},"PeriodicalIF":1.0,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143907646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative mitochondrial and phylomitogenomic analyses support the existence of a cryptic species complex in Gonodactylaceus falcatus (Stomatopoda: Gonodactyloidea)","authors":"Helen Kiser ,&nbsp;Hee-seung Hwang ,&nbsp;Jongwoo Jung ,&nbsp;J. Antonio Baeza","doi":"10.1016/j.genrep.2025.102248","DOIUrl":"10.1016/j.genrep.2025.102248","url":null,"abstract":"<div><div>Mantis shrimp (order Stomatopoda) are known for their aggressive nature and powerful raptorial appendages. Among them, the Philippine mantis shrimp <em>Gonodactylaceus falcatus</em> is suspected to comprise a cryptic species complex with 6 primary synonyms. This study aims to explore this complex focusing on <em>G. falcatus</em> ‘sensu stricto’ and three of its primary synonyms: <em>G. insularis</em>, <em>G. mutatus</em>, and <em>G. siamensis</em>. Complete mitochondrial genomes were fully assembled using Illumina sequencing. The AT-rich studied mitogenomes ranged from 15,894 to 16,267 bp in length. Each contains 13 protein-coding genes, 22 transfer RNA genes, 2 ribosomal RNA genes, and one control region. Mitochondrial synteny is identical to previously reported stomatopod mitogenomes, except <em>Gonodactylus smithii</em>. In all studied species, 21 of the 22 tRNA genes were predicted to have the typical ‘cloverleaf’ structure: trnS1 either lacked a D-arm or both a D-arm and D-loop. All mitochondrial protein-coding genes experience purifying selection, noting a Ka/Ks value for the <em>G. insularis nad4l</em> gene was not calculated due to the absence of non-synonymous substitutions. AT-rich di- and tri-nucleotide microsatellites were found in all studied control regions while short tandem repeats were detected in all except <em>G. insularis</em>. Phylomitogenomic analysis supports the monophyly of the order Stomatopoda but not the superfamilies Gonodactyloidea, Lysiosquilloidea, and Squilloidea. Furthermore, phylogenomic placement and genetic distances supported the species status of <em>G. insularis</em> and <em>G. mutatus</em> but not <em>G. siamensis</em>. Our study provides genomic resources to continue improving knowledge of cryptic species complexes in the order Stomatopoda.</div></div>","PeriodicalId":12673,"journal":{"name":"Gene Reports","volume":"40 ","pages":"Article 102248"},"PeriodicalIF":1.0,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143947060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Altered RBM10 subcellular localization relates to breast cancer via reducing p53 level and modulating immune microenvironment: Bioinformatics aided study","authors":"Sheng Huang ,&nbsp;Tairen Wang ,&nbsp;Jianbin Chen","doi":"10.1016/j.genrep.2025.102246","DOIUrl":"10.1016/j.genrep.2025.102246","url":null,"abstract":"<div><h3>Aim</h3><div>Despite significant progress in early diagnosis and treatment over the past few years, breast cancer (BC) accounts for the highest number of cancer-related deaths in the female population worldwide. Dysregulation of RBM10 has been observed in multiple types of cancers; however, the mechanism by which RBM10 alteration contributes to BC remains unclear. This study aimed to investigate the role of RBM10 in BC patients.</div></div><div><h3>Materials and methods</h3><div>Transcriptomic and proteomic data were obtained from The Cancer Genome Atlas and Clinical Proteomic Tumor Analysis Consortium. The RBM10 protein levels in tissue samples were downloaded from The Human Protein Atlas, and the phosphorylation status of RBM10 was obtained from the CPTAC database. The phosphorylation and subcellular localization of RBM10 were detected in BC cell lines. The impact of RBM10 on carcinogenesis was examined in BC cell lines using the MTT assay, flow cytometry analysis, and a xenograft mouse model. The role of RBM10 in the TME was analyzed using tumor and immune system interaction databases (TISIDB).</div></div><div><h3>Results</h3><div>Both the mRNA and protein levels of RBM10 were significantly upregulated in BC samples. After analyzing the survival data of 2976 patients with BC, we noticed that patients with higher RBM10 expression had a prolonged survival time. RBM10 protein leaked from the cell nucleus to the cytoplasm in the BC samples. Phosphorylation of S775 is a key factor modulating RBM10 subcellular localization in BC cells and regulating p53 degradation. RBM10 may function as an antitumor activator by repressing the expression of TGFBR1 and CD274, limiting the proportion of Th2 cells, and increasing the number of NK cells.</div></div><div><h3>Conclusion</h3><div>We identified for the first time that the re-localization of RBM10 is related to BC by regulating p53 degradation and modulating the immune microenvironment, which provides clues for improving the clinical treatment of BC.</div></div>","PeriodicalId":12673,"journal":{"name":"Gene Reports","volume":"40 ","pages":"Article 102246"},"PeriodicalIF":1.0,"publicationDate":"2025-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143934877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunomodulatory effects of Lactobacillus salivarius on inflammatory pathways and cytokine expression in gastric adenocarcinoma cells","authors":"Javad Allahverdy ,&nbsp;Sina Habibi ,&nbsp;Mohamad Ali Zonobian ,&nbsp;Monireh Mohsenzadegan ,&nbsp;Niloufar Rashidi","doi":"10.1016/j.genrep.2025.102249","DOIUrl":"10.1016/j.genrep.2025.102249","url":null,"abstract":"<div><div>Gastric cancer is a leading cause of cancer-related deaths globally, with chronic inflammation, influenced by factors like diet, <em>Helicobacter pylori</em> infection and chronic inflammation. Nuclear factor κ B (NF-κB), a regulator of immune responses, contributes to inflammation and tumour development, with its activation and myeloid differentiation factor 88 (MyD88) signalling influenced by microbial and environmental factors, modulating interleukins like interleukins-8 (IL-8) and interleukins-10 (IL-10). Probiotics such as <em>Lactobacillus salivarius</em> have immunomodulatory effects, potentially impacting cancer progression. This study examined <em>L. salivarius</em> cell-free supernatant (CFS) effects on interleukin expression in a gastric adenocarcinoma cell model (AGS). As results, <em>NF-κB</em> expression decreased at all doses, while <em>MyD88</em> expression increased. <em>IL-8</em> gene expression upregulated dose-dependently but was not statistically significant, while <em>IL-10</em> expression significantly increased at IC20. IL-8 protein levels increased significantly across doses, and IL-10 protein levels showed significant increases except at IC80. Overall, these results indicate that CFS can modulate MyD88, IL-8, and IL-10 in an NF-κB-independent manner.</div></div>","PeriodicalId":12673,"journal":{"name":"Gene Reports","volume":"40 ","pages":"Article 102249"},"PeriodicalIF":1.0,"publicationDate":"2025-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143918263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}