耐碳青霉烯铜绿假单胞菌的流行、分子特征及blaNDM基因的出现

IF 0.9 Q4 GENETICS & HEREDITY

Gene Reports Pub Date : 2025-09-26 DOI:10.1016/j.genrep.2025.102346

Samira Ahmadi Asli , Shabnam Golbouy Daghdari , Farzin Asghari-Sana , Mohammad Sarkheili

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引用次数: 0

摘要

铜绿假单胞菌是医院获得性感染的主要原因，具有越来越多的耐多药性。本研究旨在评估临床分离菌株的耐药模式，评估表型金属β-内酰胺酶（MBL）检测，并研究关键耐药基因（blaNDM、blaIMP、blaVIM和blaOXA-10）。从伊朗乌尔米娅的三家大医院共收集了472份临床样本，鉴定出81株铜绿假单胞菌确诊菌株。采用纸片扩散法检测抗菌药物敏感性，通过CDT-IPM、DDST-IPM、CDT-CAZ和DDST-CAZ四种表型试验评估MBL的产生。采用单链和多重PCR鉴定耐药基因。头孢西丁（93.8%）和美罗培南（60.0%）的耐药率最高，粘菌素和阿米卡星的耐药率最高。在表型试验中，DDST-IPM表现出最高的敏感性（100%），但特异性有限（57%）。CDT-IPM表现出可比的特异性。相比之下，CDT-CAZ和DDST-CAZ表现出较低的特异性和可变敏感性。PCR结果显示，85.7%的碳青霉烯类耐药菌株中存在blaOXA-10，其次是blaVIM（73.5%）、blaIMP（53.0%）和blaNDM（38.8%）。对粘菌素耐药菌株的检测和多个MBL基因的共存引起了对治疗局限性的关注，并突出了更好的诊断和耐药性监测的必要性。

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Prevalence and molecular characterization of carbapenem-resistant Pseudomonas aeruginosa and emergence of blaNDM gene

Pseudomonas aeruginosa is a major cause of hospital-acquired infections, with increasing multidrug resistance. This study aimed to evaluate antimicrobial resistance patterns, assess phenotypic metallo-β-lactamase (MBL) detection, and investigate key resistance genes (bla_NDM, bla_IMP, bla_VIM, and bla_OXA-10) in clinical isolates. A total of 472 clinical samples were collected from three major hospitals in Urmia, Iran, resulting in the identification of 81 confirmed P. aeruginosa isolates. Antimicrobial susceptibility was tested using the disk diffusion method, and MBL production was assessed through four phenotypic tests (CDT-IPM, DDST-IPM, CDT-CAZ, and DDST-CAZ). Resistance genes were confirmed using both uniplex and multiplex PCR. The highest resistance was observed against cefoxitin (93.8 %) and meropenem (60.0 %), while colistin and amikacin remained the most effective agents. Among the phenotypic tests, DDST-IPM demonstrated the highest sensitivity (100 %), but showed limited specificity (57 %). CDT-IPM showed comparable specificity. In contrast, CDT-CAZ and DDST-CAZ exhibited lower specificity and variable sensitivity. PCR results showed that bla_OXA-10 was present in 85.7 % of carbapenem-resistant isolates, followed by bla_VIM (73.5 %), bla_IMP (53.0 %), and bla_NDM (38.8 %). The detection of colistin-resistant strains and the co-occurrence of multiple MBL genes raise concerns about treatment limitations and highlight the need for better diagnostics and resistance monitoring.

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来源期刊

Gene Reports Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

3.30

自引率

7.70%

发文量

246

审稿时长

49 days

期刊介绍： Gene Reports publishes papers that focus on the regulation, expression, function and evolution of genes in all biological contexts, including all prokaryotic and eukaryotic organisms, as well as viruses. Gene Reports strives to be a very diverse journal and topics in all fields will be considered for publication. Although not limited to the following, some general topics include: DNA Organization, Replication & Evolution -Focus on genomic DNA (chromosomal organization, comparative genomics, DNA replication, DNA repair, mobile DNA, mitochondrial DNA, chloroplast DNA). Expression & Function - Focus on functional RNAs (microRNAs, tRNAs, rRNAs, mRNA splicing, alternative polyadenylation) Regulation - Focus on processes that mediate gene-read out (epigenetics, chromatin, histone code, transcription, translation, protein degradation). Cell Signaling - Focus on mechanisms that control information flow into the nucleus to control gene expression (kinase and phosphatase pathways controlled by extra-cellular ligands, Wnt, Notch, TGFbeta/BMPs, FGFs, IGFs etc.) Profiling of gene expression and genetic variation - Focus on high throughput approaches (e.g., DeepSeq, ChIP-Seq, Affymetrix microarrays, proteomics) that define gene regulatory circuitry, molecular pathways and protein/protein networks. Genetics - Focus on development in model organisms (e.g., mouse, frog, fruit fly, worm), human genetic variation, population genetics, as well as agricultural and veterinary genetics. Molecular Pathology & Regenerative Medicine - Focus on the deregulation of molecular processes in human diseases and mechanisms supporting regeneration of tissues through pluripotent or multipotent stem cells.