对恶性疟原虫msp1和msp2候选疫苗多态性的调查显示，喀麦隆遗传多样性单克隆感染的比例很高

IF 0.9 Q4 GENETICS & HEREDITY

Gene Reports Pub Date : 2025-10-01 DOI:10.1016/j.genrep.2025.102348

Loick P. Kojom Foko , Joseph Hawadak , Vineeta Singh

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引用次数: 0

摘要

疟疾免疫在很大程度上受疟原虫种群遗传结构的调节，这是成功研制疫苗的一个重要障碍。方法采集4个镇（杜阿拉、马鲁阿、梅奥奥洛和佩特）医院患者的干血斑。提取基因组DNA，对裂殖子表面蛋白1和2基因（msp1和msp2）进行基因分型。结果msp1和msp2等位基因型分别为sk1（76.3%）和IC/3D7（83.3%）。基因型总数为24个msp1（11个K1； 8个Mad20； 5个RO33）和43个msp2（29个IC/3D7； 14个FC27）。msp1 [K1（46.1%）、Mad20（18.4%）、RO33(3.9%)]单克隆感染占68.4%,msp2 [IC/3D7（50.0%）、FC27(17.1%)]单克隆感染占67.1%。居住在Pette的个体获得msp1相关单克隆感染的机会低于居住在Douala的个体（OR = 0.22, p = 0.01）。msp2在MI中发现的IC/3D7和FC27基因型分别占IC/3D7和FC27基因型总数的58.6%和100%。MOI与年龄、寄生虫密度和地理区域无统计学意义相关。结论恶性疟原虫感染具有高度的遗传多样性，且以单克隆感染为主。

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Investigating polymorphism of Plasmodium falciparum msp1 and msp2 vaccine candidates reveals a high proportion of genetically diverse monoclonal infections in Cameroon

Background

Antimalarial immunity is greatly modulated by the genetic structure of Plasmodium populations, which is an important hurdle to successful vaccine development.

Methods

Dried blood spots were collected from hospital patients living in four towns (Douala, Maroua, Mayo-Oulo, and Pette). Genomic DNA was extracted and used to genotype the merozoite surface protein 1 and 2 genes (msp1 and msp2).

Results

K1 (76.3 %) and IC/3D7 (83.3 %) were the most frequently found msp1 and msp2 allelic types, respectively. The total number of genotypes was 24 msp1 (11 K1; 8 Mad20; 5 RO33) and 43 msp2 (29 IC/3D7; 14 FC27). Monoclonal infections were predominant: 68.4 % for msp1 [K1 (46.1 %), Mad20 (18.4 %), and RO33 (3.9 %)], and 67.1 % for msp2 [IC/3D7 (50.0 %), and FC27 (17.1 %)]. Individuals living in Pette had fewer chances to get msp1-related monoclonal infections than those living in Douala (OR = 0.22, p = 0.01). Regarding msp2, IC/3D7 and FC27 genotypes found in MI accounted for 58.6 % and 100 % of total IC/3D7 and FC27 genotypes found. No statistically significant association was found between MOI, age, parasite density, and geographical area.

Conclusion

This study reveals a high genetic diversity of P. falciparum infections, with a predominance of monoclonal infections.

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来源期刊

Gene Reports Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

3.30

自引率

7.70%

发文量

246

审稿时长

49 days

期刊介绍： Gene Reports publishes papers that focus on the regulation, expression, function and evolution of genes in all biological contexts, including all prokaryotic and eukaryotic organisms, as well as viruses. Gene Reports strives to be a very diverse journal and topics in all fields will be considered for publication. Although not limited to the following, some general topics include: DNA Organization, Replication & Evolution -Focus on genomic DNA (chromosomal organization, comparative genomics, DNA replication, DNA repair, mobile DNA, mitochondrial DNA, chloroplast DNA). Expression & Function - Focus on functional RNAs (microRNAs, tRNAs, rRNAs, mRNA splicing, alternative polyadenylation) Regulation - Focus on processes that mediate gene-read out (epigenetics, chromatin, histone code, transcription, translation, protein degradation). Cell Signaling - Focus on mechanisms that control information flow into the nucleus to control gene expression (kinase and phosphatase pathways controlled by extra-cellular ligands, Wnt, Notch, TGFbeta/BMPs, FGFs, IGFs etc.) Profiling of gene expression and genetic variation - Focus on high throughput approaches (e.g., DeepSeq, ChIP-Seq, Affymetrix microarrays, proteomics) that define gene regulatory circuitry, molecular pathways and protein/protein networks. Genetics - Focus on development in model organisms (e.g., mouse, frog, fruit fly, worm), human genetic variation, population genetics, as well as agricultural and veterinary genetics. Molecular Pathology & Regenerative Medicine - Focus on the deregulation of molecular processes in human diseases and mechanisms supporting regeneration of tissues through pluripotent or multipotent stem cells.