Gene Reports最新文献

{"title":"Sperm non-coding RNAs and pregnancy outcomes: A literature review","authors":"Parisa Dolati,&nbsp;Emran Esmaeilzadeh,&nbsp;Hamid Reza Khorram Khorshid","doi":"10.1016/j.genrep.2025.102420","DOIUrl":"10.1016/j.genrep.2025.102420","url":null,"abstract":"<div><div>The spermatozoon is a specialized cell that transmits not only DNA but also other molecules, which, until recently, were thought to transport the paternal genome to the oocyte solely. Recent evidences show that the sperm is responsible for delivering many other components and molecules that are essential not only in early embryonic events but might also have long-lasting effects across several generations. Non-coding RNAs (ncRNAs) carried by sperm have been implicated in fertilization, embryo and placental development, and offspring health. Meanwhile, non-coding RNAs (ncRNAs) are known to be part of dynamic epigenetic mechanisms that can rapidly change in response to various internal and external factors. Here, we provide a clinician-oriented synthesis emphasizing paternal evidence, mechanisms, and actionable gaps. We summarize insights from the current understanding of sperm epigenetic information, particularly ncRNAs, their impact on pregnancy outcomes, placental growth, development, and the possible pathways involved.</div></div>","PeriodicalId":12673,"journal":{"name":"Gene Reports","volume":"42 ","pages":"Article 102420"},"PeriodicalIF":0.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145880405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MNK1 affects thermal tolerance capability via SNP alteration in its coding sequence region in scallops (Patinopecten yessoensis)","authors":"Shaohua Liu ,&nbsp;Jingsong Wang ,&nbsp;Aoyu Fang ,&nbsp;Linghui Yu ,&nbsp;Huiqi Deng ,&nbsp;Yaqing Chang ,&nbsp;Yaoyao Zhan","doi":"10.1016/j.genrep.2025.102407","DOIUrl":"10.1016/j.genrep.2025.102407","url":null,"abstract":"<div><div>To clarify the relationship between <em>MNK1</em> gene expression and thermal tolerance capability in scallops, high-temperature (24 °C) treatment was performed to obtain high-temperature intolerance individuals (HN) and high-temperature tolerance individuals (HR) from the same lineage of the scallop <em>Patinopecten yessoensis</em>. Correlation of <em>MNK1</em> gene expression and thermal tolerance capability was preliminary established by determining relative expression of <em>MNK1</em> in both HN and HR groups. To further dissect the possible mechanism that <em>MNK1</em> expression affects thermal tolerance capability in <em>P. yessoensis</em>, regular PCR (polymerase chain reaction) amplification and bioinformatics analyses were employed to compare structures of <em>MNK1</em> coding sequence (CDS), predicted <em>MNK1</em> transcript, and predicted MNK1 protein between HN and HR groups. The results showed that: 1) the relative expression of <em>MNK1</em> gene in the gills of HR group was significantly lower than that of HN group (<em>P</em> &lt; 0.05); 2) CDS analysis of <em>MNK1</em> gene showed that there are three SNP sites at positions 652 (c.652 A &gt; G), 659 (c.659 C &gt; A), and 686 (c.686 C &gt; A) of <em>MNK1</em> CDS were different between HN and HR groups. Further analyses showed that all three identified SNPs exhibited a moderate polymorphism (0.25 ≤ PIC &lt;0.5) with a distribution in line with the Hardy-Weinberg equilibrium state (<em>P</em> &gt; 0.05). Compared with HN, the dominant genotype of the identified SNPs were AG (c.652 A &gt; G), CA (c.659 C &gt; A), and CA (c.686 C &gt; A) in CDS of <em>MNK1</em> gene of HR group. Bioinformatics analyses indicate that the predicted secondary structure of the MNK1 mRNA, the amino acid composition and the spatial structure of MNK1 protein could be altered when the c.686 C &gt; A site genotype changing from CC to <em>CA.</em> In conclusion, the results obtained in this study not only clarified the association between the <em>MNK1</em> gene expression and the high-temperature tolerance capability in <em>P. yessoensis</em>, but also provide novel biomarkers for selective breeding of <em>P. yessoensis</em> with high-temperature tolerance trait.</div></div>","PeriodicalId":12673,"journal":{"name":"Gene Reports","volume":"42 ","pages":"Article 102407"},"PeriodicalIF":0.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145733279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association between Wnt signaling-related gene expression and clinical features in Iranian colorectal cancer patients: A potential diagnostic insight","authors":"Mostafa Zargar ,&nbsp;Ahdieh Hosseini Anvari ,&nbsp;Mohammad Hadi Abbasian ,&nbsp;Amir Hossein Alipour ,&nbsp;Reza Shirkoohi ,&nbsp;Shahla Mohammad Ganji","doi":"10.1016/j.genrep.2025.102395","DOIUrl":"10.1016/j.genrep.2025.102395","url":null,"abstract":"<div><div>Aberrant activation of the Wnt signaling pathway plays a central oncogenic role in colorectal cancer (CRC). However, the expression profiles and clinical significance of multiple Wnt-related genes remain underexplored, particularly in specific populations such as Iranian patients. This study aimed to evaluate the expression of six key Wnt-associated genes and their associations with clinicopathological features in CRC tissues.</div><div>A case-control study was conducted using 40 advanced colorectal carcinoma tissues and 35 adjacent normal tissues obtained from Imam Khomeini Hospital, Tehran (2016–2017). Total RNA was extracted and reverse-transcribed, followed by quantitative real-time PCR. Gene expression levels were normalized to β-actin as the reference gene, and correlations with TNM staging parameters were analyzed statistically.</div><div>Five genes (HIF-1α, GSK3β, BCL9, CCND1, CDK1) were significantly upregulated in CRC tissues compared to normal controls (all <em>P</em> &lt; 0.05), whereas MLH1 was downregulated (<em>P</em> = 0.052, borderline significance). MLH1 expression showed significant negative correlations with tumor stage, lymph node involvement (N), and metastasis (M) (all <em>P</em> = 0.02). GSK3β expression correlated with metastatic status (<em>P</em> = 0.002), BCL9 with tumor size (T) and nodal involvement (<em>P</em> &lt; 0.05), and HIF-1α with tumor stage and nodal status (P &lt; 0.05).</div><div>This multi-gene expression analysis reveals coordinated dysregulation of Wnt pathway components in CRC, with distinct correlation patterns linking specific genes to disease progression markers. These findings suggest potential applications in molecular staging and therapeutic targeting for Iranian CRC patients.</div></div>","PeriodicalId":12673,"journal":{"name":"Gene Reports","volume":"42 ","pages":"Article 102395"},"PeriodicalIF":0.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145733250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptional regulation of Toll-like Receptors in Pinna nobilis: Insights into immune response under varying pathogen loads and effect of individual variation","authors":"Athanasios Lattos ,&nbsp;Stéphane Coupé ,&nbsp;Dimitrios K. Papadopoulos ,&nbsp;Ioannis A. Giantsis","doi":"10.1016/j.genrep.2025.102415","DOIUrl":"10.1016/j.genrep.2025.102415","url":null,"abstract":"<div><div>Scientific community is currently witnessing one of the most dynamic extinction risks, i.e. that of the largest bivalve mollusk of the Mediterranean, <em>Pinna nobilis</em>. The role of possible different pathogens responsible for the mass mortalities driving this extinction risk represent a considerable debate among researchers. After extensive investigation of the etiological agents, by means of numerous microbiological, pathophysiological, environmental studies, as well as efforts to identify surviving populations, current research is attempting to focus on the characterization of resistant genotypes that could enable future recruitment. Recently, the first characterization of the genomic architecture of Toll-like receptor (TLRs) genes in the genus <em>Pinna</em> identified potential SNPs associated with disease resistance and, hence, with genotypes tolerant to mortalities. Nevertheless, gene characterization is a challenging task that requires investigation at multiple levels, including gene expression under different stressors. In this context, the present study investigates TLR gene transcription in <em>Pinna nobilis</em> populations infected by different pathogens, comparing single infection with co-infection. In a general agreement with the proposed role of TLR4 and TLR6 which host SNPs associated with resistant or susceptible <em>Pinna</em> genotypes, the current results demonstrate a statistically significant increased transcription of these genes in individuals infected with both <em>Mycobacterium</em> sp. and <em>Haplosporidium pinnae</em>, the two mostly accused pathogens, compared with specimens infected with <em>Mycobacterium</em> sp. solely. Since analyses were only based on mantle tissue, these transcriptional associations require confirmation by multi-tissue and protein-level studies to clarify the functional role of TLRs in <em>Pinna nobilis</em> immunity and disease resistance. To this end, after examination of a large number, 11 primer pairs were validated in terms of efficiency and stability and are proposed for usage in other populations as well.</div></div>","PeriodicalId":12673,"journal":{"name":"Gene Reports","volume":"42 ","pages":"Article 102415"},"PeriodicalIF":0.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145836429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reduced H3C6 mRNA levels in preeclamptic placentas without associated CpG island methylation changes","authors":"Majid Zaki-Dizaji ,&nbsp;Mohsen Saravani ,&nbsp;Behnoosh Jamshidi ,&nbsp;Seyed Mohammad-Hossein Nemati ,&nbsp;Marzieh Ghasemi ,&nbsp;Zohreh Heidary","doi":"10.1016/j.genrep.2025.102419","DOIUrl":"10.1016/j.genrep.2025.102419","url":null,"abstract":"<div><h3>Background</h3><div>Despite advances in diagnostics, preeclampsia (PE) remains a leading cause of maternal and fetal morbidity. Elucidating the molecular mechanisms underlying PE is essential for developing improved interventions. Recent evidence points to epigenetic dysregulation of HIST1H3E (H3C6) in PE placentas, characterized by decreased expression and hypermethylation. Therefore, this study investigates H3C6 expression and methylation patterns in PE placentas compared to healthy controls.</div></div><div><h3>Materials and methods</h3><div>We analyzed 30 PE and 30 control placental tissues. <em>H3C6</em> mRNA levels were quantified by RT-qPCR, and methylation status was assessed via Methylation-Quantification of Endonuclease-Resistant DNA (MethyQESD) targeting two CpG island regions in H3C6 gene. Statistical analyses were performed using GraphPad Prism.</div></div><div><h3>Results</h3><div>Quantitative analysis revealed significantly reduced H3C6 mRNA expression in preeclamptic placentas compared to controls (<em>p</em> &lt; 0.05). However, methylation analysis of two CpG island regions within H3C6 gene demonstrated no significant differences between groups (<em>p</em> &gt; 0.05).</div></div><div><h3>Conclusion</h3><div>While H3C6 is underexpressed in PE, its methylation status in the analyzed CpG islands remains unaltered. Future studies should explore other regulatory regions (e.g., CpG shores/shelfs) and mechanistic links to PE pathophysiology.</div></div>","PeriodicalId":12673,"journal":{"name":"Gene Reports","volume":"42 ","pages":"Article 102419"},"PeriodicalIF":0.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145836485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In silico and in vitro cytotoxic effects of amphotericin B and its niosomal form on human umbilical vein endothelial cells: Exploration of apoptosis-related pathways","authors":"Fatemeh Sharifi ,&nbsp;Razieh Tavakoli Oliaee ,&nbsp;Mehdi Bamorovat ,&nbsp;Ahmad Khosravi ,&nbsp;Abbas Pardakhty ,&nbsp;Iraj Sharifi","doi":"10.1016/j.genrep.2026.102429","DOIUrl":"10.1016/j.genrep.2026.102429","url":null,"abstract":"<div><div>This study evaluated the effects of amphotericin B (AmB) and its niosomal formulation (N-AmB) on human umbilical vein endothelial cells (HUVECs) as a sensitive model for monitoring vascular endothelial cell cytotoxicity. Cytotoxicity assessments, apoptotic analysis, and gene expression profiling were performed using colorimetric assays, flow cytometric analysis, and quantitative real-time polymerase chain reaction (qPCR), respectively. In silico analyses showed an affinity of AmB to caspases 3/9, with the MolDock score of −300.26 and -240.47, respectively. The prepared vesicles exhibited a mean particle diameter of 14.3 ± 0.62 μm (mean ± SD, <em>n</em> = 3), i.e., they are microscale niosomal vesicles rather than nanoscale niosomes; this distinction has implications for intravenous delivery and macrophage uptake. The measured zeta potential was −13.23 ± 0.49 mV. It was determined that N-AmB had an encapsulation efficiency of almost 80%. Cytotoxicity and gene expression analyses demonstrated that N-AmB exhibited lower cytotoxicity and apoptosis levels, accompanied by reduced expression of pro-apoptotic genes and increased expression of anti-apoptotic genes compared with AmB. These results may aid in the development of safer and more effective therapeutic approaches for treating infectious diseases.</div></div>","PeriodicalId":12673,"journal":{"name":"Gene Reports","volume":"42 ","pages":"Article 102429"},"PeriodicalIF":0.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146073918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Computational exploration of novel (p.L328P) and reported (p.R209Q) variants identified in Glial Fibrillary Acidic Protein (GFAP) gene in a patient with Alexander disease from India","authors":"Surajit Malakar ,&nbsp;Tathagata Das ,&nbsp;Tamali Halder ,&nbsp;Vikrant Yadav ,&nbsp;Anurag Sahu ,&nbsp;Parimal Das","doi":"10.1016/j.genrep.2025.102393","DOIUrl":"10.1016/j.genrep.2025.102393","url":null,"abstract":"<div><h3>Background</h3><div>Alexander disease (AxD) is a rare, progressive and fatal neurological disorder characterized by degeneration of the white matter of the brain accompanied by the formation of Rosenthal fibers, distinct cytoplasmic inclusion within astrocytes (non-neuronal cells) in the brain. The disease phenotype is commonly identified to be associated with heterozygous <em>de novo</em> variation in the Glial Fibrillary Acidic Protein (<em>GFAP</em>) gene. Rare instances of familial AxD have also been reported with genetic anticipation.</div></div><div><h3>Methods and result</h3><div>The study aimed to determine the genetic cause of a clinically diagnosed case of juvenile AxD from India with macrocephaly and psychomotor delay, followed by regression, spastic paraparesis, and feeding difficulties. DNA was extracted from peripheral blood sample and was taken for whole-exome-sequencing. Two pathogenic heterozygous missense variations were identified in <em>GFAP,</em> the potential candidate for AxD (c.626G &gt; A leading to p.R209Q and c.983 T &gt; C leading to p.L328P), notably, p.L328P is being reported here first time. The karyotype of the proband revealed no chromosomal anomalies. Following confirmation by Sanger sequencing, variants including their cumulative effect in Double Mutant were characterized <em>in silico</em> for prediction of pathogenicity, protein stability, physiochemical analysis, molecular simulation, principal component analysis and molecular docking. Collectively, these findings reveal both compensatory and synergistic effects that may influence intermediate filament dynamics and related signaling pathways.</div></div><div><h3>Conclusion</h3><div>This altered GFAP protein gets accumulated in the cytoplasm of the astrocyte cells, leading to the formation of Rosenthal fibers, which impairs cell function. While <em>in silico</em> analysis supports the pathogenic nature of the studied variants, which is consistent with the observed AxD pathophysiology in the current study.</div></div>","PeriodicalId":12673,"journal":{"name":"Gene Reports","volume":"42 ","pages":"Article 102393"},"PeriodicalIF":0.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145621827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First Moroccan case of GM3 synthase deficiency identifies c.1255 T > C as a putative north African founder mutation in ST3GAL5","authors":"Azzeddine Laaraje ,&nbsp;Abdelilah Radi ,&nbsp;Abdelhakim Ourrai ,&nbsp;Amale Hassani ,&nbsp;Rachid Abilkassem","doi":"10.1016/j.genrep.2026.102428","DOIUrl":"10.1016/j.genrep.2026.102428","url":null,"abstract":"<div><div>Ganglioside GM3 synthase deficiency (GM3SD) is an ultra-rare autosomal recessive disorder caused by biallelic variants in <em>ST3GAL5</em>. We report the first Moroccan case of GM3SD and identify c.1255 T &gt; C as a putative North African founder mutation, expanding our understanding of regional genetic architecture of this disorder. A 4-year-old Moroccan boy born to first-degree consanguineous parents presented with severe global developmental delay, progressive microcephaly, hypotonia, and visual impairment evident from birth. Whole-exome sequencing of the proband revealed a homozygous stop-loss variant in <em>ST3GAL5</em>: c.1255 T &gt; C (p.Ter419ArgextTer38), resulting in read-through translation and a 38-amino acid C-terminal protein extension. Notably, this identical variant was previously reported in two Algerian patients, suggesting a founder effect specific to North African populations. The recurrence of this stop-loss variant across three unrelated families from Morocco and Algeria points to identity-by-descent inheritance from a common ancestor, representing the first regional founder mutation described for GM3SD outside the Amish population. The patient's severe clinical phenotype—including profound developmental delay, complete absence of motor milestones and language, marked microcephaly, cortical visual impairment, and elevated lactate—is consistent with complete loss of GM3 synthase function. This case has important implications for genetic counseling and carrier screening in North African populations where consanguineous marriages are prevalent, and underscores the importance of considering GM3SD in families presenting with early-onset severe encephalopathy in this region.</div></div>","PeriodicalId":12673,"journal":{"name":"Gene Reports","volume":"42 ","pages":"Article 102428"},"PeriodicalIF":0.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145973147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic characterization and bioactive potential of a Kitasatospora sp. from the rhizosphere of Inga edulis","authors":"Rafael de Souza Rodrigues ,&nbsp;Antonia Queiroz Lima de Souza ,&nbsp;Jania Lilia da Silva Bentes ,&nbsp;Elison de Souza Sevalho ,&nbsp;Kamila Rangel Primo Fernandes ,&nbsp;Anderson Nogueira Barbosa ,&nbsp;Maria de Fátima Oliveira Almeida ,&nbsp;Rafael Pinto e Souza ,&nbsp;Roneres Deniz Barbosa ,&nbsp;Jeferson Chagas da Cruz ,&nbsp;Ivanildes dos Santos Bastos ,&nbsp;Patrícia Puccinelli Orlandi ,&nbsp;Gilvan Ferreira da Silva ,&nbsp;Afonso Duarte Leão de Souza","doi":"10.1016/j.genrep.2025.102381","DOIUrl":"10.1016/j.genrep.2025.102381","url":null,"abstract":"<div><div>The genus <em>Kitasatospora</em> (phylum Actinobacteria) includes over 44 recognized species, yet their potential to produce bioactive compounds remains largely unexplored. In this study, we report the isolation and characterization of <em>Kitasatospora</em> sp. LaBMicrA B282, demonstrating its distinctiveness as an actinomycete associated with the rhizosphere of <em>Inga edulis</em> in an Amazonian urban forest fragment. The strain is Gram-positive, aerobic, and forms brownish-white aerial and grayish-brown substrate mycelia on ISP2+starch medium. The BLAST analysis of the 16S rRNA gene (&lt;99 %% identity) and low genomic relatedness (ANI: 82.86–83.42 %; dDDH2: 27.20–29.60 %; dDDH4: 27.1–27.2 %) support its classification as a distinct species within the genus. Genome mining via antiSMASH identified 63 putative biosynthetic gene clusters (BGCs), including clusters with 100 % similarity to those for geosmin, 2-methylisoborneol, and ε-poly-<span>l</span>-lysine. Bioactivity assays revealed that the aqueous supernatant fraction displayed antiplasmodial activity against <em>Plasmodium falciparum</em> W2 (IC₅₀ = 36.10 μg/mL), while the AcOEt/2-propanol fraction showed fungicidal activity against four pathogenic fungi. These results highlight the remarkable biosynthetic and therapeutic potential of <em>Kitasatospora</em> strains from underexplored environments. Our findings emphasize the importance of investigating microbial diversity in unique ecological niches like the Amazonian rhizosphere and reinforce the need to conserve these environments as reservoirs of novel bioactive compounds.</div></div>","PeriodicalId":12673,"journal":{"name":"Gene Reports","volume":"42 ","pages":"Article 102381"},"PeriodicalIF":0.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145733280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An update on TWEAK/Fn14 signaling pathway: A comprehensive review","authors":"Ujjawal Sharma ,&nbsp;Arpit Mehrotra ,&nbsp;Bunty Sharma ,&nbsp;Abhilasha Sood ,&nbsp;Kartin Sak ,&nbsp;Shafiul Haque ,&nbsp;Nidarshana Chaturvedi Parashar ,&nbsp;Madhu Gupta ,&nbsp;Hardeep Singh Tuli","doi":"10.1016/j.genrep.2025.102403","DOIUrl":"10.1016/j.genrep.2025.102403","url":null,"abstract":"<div><div>Tumor necrosis factor-like weak inducer of apoptosis (TWEAK) is a multifunctional protein, present in various cell types and tissues, which, upon binding to the receptor factor-inducible 14 (Fn14), is involved in diverse pathologic processes including cell proliferation and death, angiogenesis, carcinogenesis, and inflammation. Interestingly, any alterations of their intracellular signaling are well correlated with the advancement of several diseases. The expression of TWEAK and Fn14 is augmented in many solid tumors compared with healthy tissues, thus leading to the enhancement of the proliferation, invasion, and subsequent migration of tumor cells. However, few TWEAK/Fn14 targeting pharmacological agents involved in inhibiting the progression of tumors have shown initial success in both clinical and pre-clinical experimental settings. Moreover, such agents improved angiogenesis, pro-inflammatory cytokine expression, and epithelial–mesenchymal transitions, upon TWEAK/Fn14 activation. This review provides an overview of the current understanding of the TWEAK–Fn14 signaling axis, its role in augmenting pathological characteristics of various diseases, and the existence of potential therapeutic targets for the development of novel pharmacological interventions.</div></div>","PeriodicalId":12673,"journal":{"name":"Gene Reports","volume":"42 ","pages":"Article 102403"},"PeriodicalIF":0.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145733252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}