The Survey of long non-coding RNA HOTTIP expression level in endometriosis

IF 0.9 Q4 GENETICS & HEREDITY

Gene Reports Pub Date : 2025-09-10 DOI:10.1016/j.genrep.2025.102338

Parisa Vesal , Amirhossein Rezvani Rezvandeh , Namdar Razmavar , Zivar Salehi , Farhad Mashayekhi , Kiana Sojoudi , Ziba Zahiri , Zakieh Siahpoosh

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引用次数: 0

Abstract

Background

Recent researches have highlighted the promising potential of long non-coding RNA (lncRNAs) as an important regulator in endometriosis. Accumulating shreds of evidence has shown an important role of HOTTIP (HOXA transcript at the distal tip) in cell proliferation, differentiation, and migration. The function of HOTTIP in relation to endometriosis is still not well understood.

Materials and methods

The goal of this research was to examine the expression profile of HOTTIP and HOXA genes in 70 matched eutopic endometrium (EU) and ectopic endometrium (EC) samples from women with endometriosis, with 85 normal endometrium control samples (C) from healthy women. Quantitative real-time PCR (qRT-PCR) assay was performed to measure the level of gene expression. Cell viability was assessed using the CCK-8 assay. Two endometriotic cell lines were transfected with siRNA against HOTTIP (si-HOTTIP) and then used to monitor the effect of HOTTIP on the expression of HOXA13. Bioinformatic tools and the Cytoscape platform were utilized to perform network analysis and gene enrichment analysis.

Results

Results indicated that the expression of HOXA13 and HOTTIP was elevated in EC and EU samples in comparison to the C group (p < 0.05). However, HOXA9, HOXA10, and HOXA11 showed decreased expression in EC samples compared to their levels in the C and EU samples. The expression levels of HOTTIP RNA showed a positive correlation with HOXA13 in patients with endometriosis. qRT-PCR data exhibited that si-HOTTIP triggered the reduction of both HOTTIP and HOXA13 expression levels and cell viability, and bioinformatics analysis in the Cytoscape platform helped us to make our laboratory results more reliable. In conclusion, the dysregulation of HOXA genes by HOTTIP lncRNA, characterized by the upregulation of HOXA13 and the downregulation of HOXA9, HOXA10 and HOXA11, suggests its significant role in the development of endometriosis and its potential as a therapeutic target.

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长链非编码RNA HOTTIP在子宫内膜异位症中的表达水平调查

最近的研究强调了长链非编码RNA （lncRNAs）作为子宫内膜异位症的重要调节因子的潜力。越来越多的证据表明HOTTIP（远端HOXA转录本）在细胞增殖、分化和迁移中起着重要作用。HOTTIP在子宫内膜异位症中的作用尚不清楚。材料和方法本研究的目的是检测HOTTIP和HOXA基因在子宫内膜异位症女性70例匹配的异位子宫内膜（EU）和异位子宫内膜（EC）样本和85例健康女性正常子宫内膜对照样本(C)中的表达谱。采用实时荧光定量PCR （qRT-PCR）检测基因表达水平。采用CCK-8法测定细胞活力。用siRNA （si-HOTTIP）转染2株子宫内膜异位症细胞系，检测HOTTIP对HOXA13表达的影响。利用生物信息学工具和Cytoscape平台进行网络分析和基因富集分析。结果与C组相比，EC组和EU组HOXA13和HOTTIP的表达均升高（p < 0.05）。然而，HOXA9、HOXA10和HOXA11在EC样品中的表达水平低于在C和EU样品中的表达水平。在子宫内膜异位症患者中，HOTTIP RNA的表达水平与HOXA13呈正相关。qRT-PCR数据显示，si-HOTTIP触发了HOTTIP和HOXA13表达水平和细胞活力的降低，并且在Cytoscape平台上的生物信息学分析帮助我们使我们的实验室结果更加可靠。综上所述，HOTTIP lncRNA对HOXA基因的失调，表现为HOXA13的上调，HOXA9、HOXA10和HOXA11的下调，表明其在子宫内膜异位症的发生中起着重要作用，具有潜在的治疗靶点。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Gene Reports Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

3.30

自引率

7.70%

发文量

246

审稿时长

49 days

期刊介绍： Gene Reports publishes papers that focus on the regulation, expression, function and evolution of genes in all biological contexts, including all prokaryotic and eukaryotic organisms, as well as viruses. Gene Reports strives to be a very diverse journal and topics in all fields will be considered for publication. Although not limited to the following, some general topics include: DNA Organization, Replication & Evolution -Focus on genomic DNA (chromosomal organization, comparative genomics, DNA replication, DNA repair, mobile DNA, mitochondrial DNA, chloroplast DNA). Expression & Function - Focus on functional RNAs (microRNAs, tRNAs, rRNAs, mRNA splicing, alternative polyadenylation) Regulation - Focus on processes that mediate gene-read out (epigenetics, chromatin, histone code, transcription, translation, protein degradation). Cell Signaling - Focus on mechanisms that control information flow into the nucleus to control gene expression (kinase and phosphatase pathways controlled by extra-cellular ligands, Wnt, Notch, TGFbeta/BMPs, FGFs, IGFs etc.) Profiling of gene expression and genetic variation - Focus on high throughput approaches (e.g., DeepSeq, ChIP-Seq, Affymetrix microarrays, proteomics) that define gene regulatory circuitry, molecular pathways and protein/protein networks. Genetics - Focus on development in model organisms (e.g., mouse, frog, fruit fly, worm), human genetic variation, population genetics, as well as agricultural and veterinary genetics. Molecular Pathology & Regenerative Medicine - Focus on the deregulation of molecular processes in human diseases and mechanisms supporting regeneration of tissues through pluripotent or multipotent stem cells.