Identification of a novel antimicrobial resistance associated plasmid in Pseudomonas sp. from disease tilapia (Oreochromis niloticus)

IF 1 Q4 GENETICS & HEREDITY

Gene Reports Pub Date : 2025-04-05 DOI:10.1016/j.genrep.2025.102220

Jocelyne García Armenta , Carmen E. Vargas-Peralta , Roberto Cruz-Flores , Olivia Cabanillas-Bernal , Jesús Antonio López-Carvallo , Jorge Cáceres-Martínez

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Abstract

This study identifies and characterizes a novel antimicrobial resistance-associated megaplasmid in Pseudomonas sp. isolated from diseased Nile tilapia (Oreochromis niloticus) in Mexico. Aquaculture's growth has heightened concerns over antimicrobial resistance (AMR) due to the widespread prophylactic use of antibiotics to manage bacterial infections, which contributes to AMR in pathogens like Pseudomonas spp., an opportunistic agent of fish disease. A bacterial isolate from symptomatic tilapia was identified as closely related to Pseudomonas soli through 16S rRNA and 23S rRNA gene sequencing. Whole-genome sequencing revealed a 362,580 bp megaplasmid encoding 984 genes, including multidrug resistance elements, confirmed by PCR targeting key genes (repA, parA and virB4/traC). The megaplasmid exhibited high average nucleotide identity (96 %) to known resistance plasmids, underscoring the potential for horizontal gene transfer in aquaculture environments. This first identification of such a megaplasmid in tilapia pathogens highlights the urgent need for sustainable antibiotic practices in aquaculture to mitigate AMR's spread. Our findings contribute to understanding the genomic basis of AMR in Pseudomonas sp. and offer insights for developing management strategies to safeguard fish health and food security.

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病罗非鱼假单胞菌耐药相关质粒的鉴定

本研究发现并描述了从墨西哥患病尼罗罗非鱼（Oreochromis niloticus）中分离出的假单胞菌中一种新型抗菌药耐药性相关巨型质粒。水产养殖业的发展加剧了人们对抗菌素耐药性（AMR）的担忧，这是因为人们广泛预防性地使用抗生素来控制细菌感染，从而导致像假单胞菌属这样的病原体产生抗菌素耐药性，而假单胞菌属是鱼类疾病的机会性病原体。通过 16S rRNA 和 23S rRNA 基因测序，从有症状的罗非鱼中分离出的细菌被鉴定为与溶血假单胞菌密切相关。全基因组测序发现了一个 362,580 bp 的巨型质粒，该质粒编码 984 个基因，包括耐多药元件，并通过针对关键基因（repA、parA 和 virB4/traC）的聚合酶链式反应得到了证实。该巨型质粒与已知抗性质粒的平均核苷酸同一性很高（96%），突出了水产养殖环境中基因水平转移的潜力。这是在罗非鱼病原体中首次发现这种巨型质粒，凸显了在水产养殖业中采用可持续抗生素方法以减缓 AMR 传播的迫切需要。我们的发现有助于了解假单胞菌 AMR 的基因组基础，并为制定管理策略以保障鱼类健康和食品安全提供了启示。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Gene Reports Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

3.30

自引率

7.70%

发文量

246

审稿时长

49 days

期刊介绍： Gene Reports publishes papers that focus on the regulation, expression, function and evolution of genes in all biological contexts, including all prokaryotic and eukaryotic organisms, as well as viruses. Gene Reports strives to be a very diverse journal and topics in all fields will be considered for publication. Although not limited to the following, some general topics include: DNA Organization, Replication & Evolution -Focus on genomic DNA (chromosomal organization, comparative genomics, DNA replication, DNA repair, mobile DNA, mitochondrial DNA, chloroplast DNA). Expression & Function - Focus on functional RNAs (microRNAs, tRNAs, rRNAs, mRNA splicing, alternative polyadenylation) Regulation - Focus on processes that mediate gene-read out (epigenetics, chromatin, histone code, transcription, translation, protein degradation). Cell Signaling - Focus on mechanisms that control information flow into the nucleus to control gene expression (kinase and phosphatase pathways controlled by extra-cellular ligands, Wnt, Notch, TGFbeta/BMPs, FGFs, IGFs etc.) Profiling of gene expression and genetic variation - Focus on high throughput approaches (e.g., DeepSeq, ChIP-Seq, Affymetrix microarrays, proteomics) that define gene regulatory circuitry, molecular pathways and protein/protein networks. Genetics - Focus on development in model organisms (e.g., mouse, frog, fruit fly, worm), human genetic variation, population genetics, as well as agricultural and veterinary genetics. Molecular Pathology & Regenerative Medicine - Focus on the deregulation of molecular processes in human diseases and mechanisms supporting regeneration of tissues through pluripotent or multipotent stem cells.