发布求助
文献互助 智能选刊 最新文献

Gan最新文献

筛选
英文 中文
Establishment of human KB cells resistant to 1-beta-D-arabinofuranosylcytosine, and mechanisms of cellular resistance in isolated clones. 人KB细胞对1- β - d -阿拉伯糖醛基胞嘧啶耐药的建立及分离克隆细胞耐药机制
Gan Pub Date : 1984-08-01
T Tsuruo, K Naganuma, H Iida, S Sone, K Ishii, E Tsubura, S Tsukagoshi, Y Sakurai
{"title":"Establishment of human KB cells resistant to 1-beta-D-arabinofuranosylcytosine, and mechanisms of cellular resistance in isolated clones.","authors":"T Tsuruo,&nbsp;K Naganuma,&nbsp;H Iida,&nbsp;S Sone,&nbsp;K Ishii,&nbsp;E Tsubura,&nbsp;S Tsukagoshi,&nbsp;Y Sakurai","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A subline of human KB cells that was resistant to 1-beta-D-arabinofuranosylcytosine (ara-C) was established by continuous exposure of the cells to increasing concentrations of ara-C. Thirteen resistant clones were isolated from the resistant subline (KB/ara-C). KB/ara-C showed 1,300-fold higher resistance than the parent KB cells to ara-C; the most resistant clones, clones 7 and 10, showed 1,330-fold higher resistance. In the absence of ara-C, the resistance of the parent KB/ara-C cells was stable for at least 14 weeks, whereas that of clone 7 was stable for 10 weeks, but was slightly less after 14 weeks. The ara-C kinase and ara-C deaminase activities of the 13 clones and the cellular uptake of ara-C by several clones were measured. In general the clones showed decreased deoxycytidine kinase activity and decreased cellular uptake of ara-C. Most clones had higher cytidine deaminase activity than KB cells, but some had activity similar to that of the KB cells. A clear inverse relationship was found between the ara-C sensitivity of the clones and their kinase activity, but not their deaminase activity or their ara-C uptake. These results clearly demonstrate that a major mechanism of ara-C resistance of these human KB cells was a decrease in the activity of the ara-C activating enzyme deoxycytidine kinase. The parent KB/ara-C cells showed no clear cross-resistance to various antitumor agents other than an ara-C derivative, including metabolic inhibitors, alkylating agents, DNA binders and mitotic spindle poisons.</p>","PeriodicalId":12660,"journal":{"name":"Gan","volume":"75 8","pages":"690-8"},"PeriodicalIF":0.0,"publicationDate":"1984-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17548486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Establishment and characterization of adult T-cell leukemia virus-containing B-cell lines derived from peripheral blood of adult T-cell leukemia patients. 成人t细胞白血病患者外周血含t细胞白血病病毒b细胞系的建立和鉴定。
Gan Pub Date : 1984-08-01
K Miyamoto, N Tomita, A Ishii, T Nishizaki, A Togawa
{"title":"Establishment and characterization of adult T-cell leukemia virus-containing B-cell lines derived from peripheral blood of adult T-cell leukemia patients.","authors":"K Miyamoto,&nbsp;N Tomita,&nbsp;A Ishii,&nbsp;T Nishizaki,&nbsp;A Togawa","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>B-cell lines which showed predominantly surface IgA kappa immunoglobulins were established from each of seven adult T-cell leukemia (ATL) patients. ATL virus proviral DNA was found in five of the lines. Though the chromosomal analysis of the B-cell lines yielded normal results, fresh leukemic T-cells showed chromosome abnormalities.</p>","PeriodicalId":12660,"journal":{"name":"Gan","volume":"75 8","pages":"655-9"},"PeriodicalIF":0.0,"publicationDate":"1984-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17159290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A monoclonal antibody that defines p24, a core protein of adult T-cell leukemia virus, and its precursor. 一种单克隆抗体，定义成人t细胞白血病病毒的核心蛋白p24及其前体。
Gan Pub Date : 1984-07-01
M Fujii, K Sugamura, Y Hinuma
{"title":"A monoclonal antibody that defines p24, a core protein of adult T-cell leukemia virus, and its precursor.","authors":"M Fujii,&nbsp;K Sugamura,&nbsp;Y Hinuma","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A mouse hybridoma cell line, H15, produced monoclonal antibody reacting with all the adult T-cell leukemia (ATL) virus (ATLV)-bearing cell lines but none of the ATLV-negative cell lines tested. Binding of H15 antibody to ATLV-bearing cell surfaces was specifically blocked by anti-ATLV positive human sera. Radioimmunoprecipitation analyses revealed that the antigen detected by H15 antibody was p24, a core protein of ATLV. Pulse-chase experiments using H15 antibody led to the identification of a protein, p53, which could be a precursor of p24.</p>","PeriodicalId":12660,"journal":{"name":"Gan","volume":"75 7","pages":"595-602"},"PeriodicalIF":0.0,"publicationDate":"1984-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17155364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Hydroxylation of deoxy guanosine at the C-8 position by polyphenols and aminophenols in the presence of hydrogen peroxide and ferric ion. 在过氧化氢和铁离子存在下，多酚和氨基酚在C-8位羟基化脱氧鸟苷。
Gan Pub Date : 1984-07-01
H Kasai, S Nishimura
{"title":"Hydroxylation of deoxy guanosine at the C-8 position by polyphenols and aminophenols in the presence of hydrogen peroxide and ferric ion.","authors":"H Kasai,&nbsp;S Nishimura","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Various polyphenols and aminophenols were tested for reactivity with deoxyguanosine and DNA in the presence of hydrogen peroxide and ferric ion. Deoxyguanosine was efficiently hydroxylated at the C-8 position by these treatments. In the case of DNA, strand scission was observed in addition to the hydroxylation of guanine residues.</p>","PeriodicalId":12660,"journal":{"name":"Gan","volume":"75 7","pages":"565-6"},"PeriodicalIF":0.0,"publicationDate":"1984-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17527809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cross-resistance of vincristine-resistant sublines of P388 leukemia to mitoxantrone with special emphasis on the relationship between in vitro and in vivo cross-resistance. P388白血病长春新碱耐药亚群对米托蒽醌的交叉耐药，重点探讨体内外交叉耐药的关系。
Gan Pub Date : 1984-07-01
M Inaba, K Nagashima, Y Sakurai
{"title":"Cross-resistance of vincristine-resistant sublines of P388 leukemia to mitoxantrone with special emphasis on the relationship between in vitro and in vivo cross-resistance.","authors":"M Inaba,&nbsp;K Nagashima,&nbsp;Y Sakurai","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>In vitro and in vivo cross-resistance to mitoxantrone of two vincristine-resistant sublines of P388 leukemia with different degrees of resistance in vitro was compared with that to adriamycin. A subline with a lower degree of resistance to vincristine exhibited approximately the same responses in vivo to mitoxantrone and adriamycin as the original P388 leukemic cell line, although it was evidently cross-resistant in vitro to these agents. Another subline having a higher degree of resistance to vincristine showed a 25-fold cross-resistance in vitro and gave no response in vivo to adriamycin. With mitoxantrone, on the other hand, this subline was solidly resistant as compared with the sensitive line but still retained significant responsiveness in vivo irrespective of a 150-fold cross-resistance in vitro. These results suggest that cross-resistance on a cellular basis does not necessarily correspond to in vivo cross-resistance. The relationship between in vitro and in vivo cross-resistance is discussed.</p>","PeriodicalId":12660,"journal":{"name":"Gan","volume":"75 7","pages":"625-30"},"PeriodicalIF":0.0,"publicationDate":"1984-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17527487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Mortality from carcinoma after partial gastrectomy. 胃癌部分切除术后的死亡率。
Gan Pub Date : 1984-07-01
K Inokuchi, S Tokudome, M Ikeda, M Kuratsune, H Ichimiya, N Kaibara, T Ikejiri, N Oka
{"title":"Mortality from carcinoma after partial gastrectomy.","authors":"K Inokuchi,&nbsp;S Tokudome,&nbsp;M Ikeda,&nbsp;M Kuratsune,&nbsp;H Ichimiya,&nbsp;N Kaibara,&nbsp;T Ikejiri,&nbsp;N Oka","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The mortality from cancer, excluding gastric stump cancer, was examined in 3,827 Japanese patients who had undergone partial gastrectomy for benign gastroduodenal diseases. Although no increase in gastric stump cancer had been found in a previous analysis, the number of deaths from liver, lung and colorectal cancer was significantly greater than expected. The mortality rate was also significantly increased in patients with cirrhosis of the liver. Postoperative hepatitis, intestinal stasis and/or increased bacterial growth after Billroth II gastrectomy were considered as possible causes of the high mortality.</p>","PeriodicalId":12660,"journal":{"name":"Gan","volume":"75 7","pages":"588-94"},"PeriodicalIF":0.0,"publicationDate":"1984-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17527812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Inhibition by hemin of dinitropyrene-induced mutagenesis in Chinese hamster V79 cells. 血红素对二硝基芘诱导的中国仓鼠V79细胞诱变的抑制作用。
Gan Pub Date : 1984-07-01
Y Katoh, S Takayama, K Shudo
{"title":"Inhibition by hemin of dinitropyrene-induced mutagenesis in Chinese hamster V79 cells.","authors":"Y Katoh,&nbsp;S Takayama,&nbsp;K Shudo","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The inhibitory effects of hemin on the mutagenic activities of 1,3-, 1,6- and 1,8-dinitropyrenes (1,3-,1,6- and 1,8-DNPs) were investigated in Chinese hamster V79 cells. Mutant cells were selected on the basis of their resistance to ouabain. Hemin itself did not have any cytotoxic effect on Chinese hamster V79 cells, and did not induce mutations when added at a concentration of 10 micrograms/ml. The mutagenic activities of 1,3-, 1,6- and 1,8-DNPs were inhibited dose-dependently by hemin and were reduced 91.7%, 95.7% and 94.7%, respectively, by the highest concentration of hemin tested (10 micrograms/ml).</p>","PeriodicalId":12660,"journal":{"name":"Gan","volume":"75 7","pages":"574-7"},"PeriodicalIF":0.0,"publicationDate":"1984-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17527810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Prediction of male lung cancer mortality in Japan based on birth cohort analysis. 基于出生队列分析的日本男性肺癌死亡率预测。
Gan Pub Date : 1984-07-01
N Hamajima, K Aoki
{"title":"Prediction of male lung cancer mortality in Japan based on birth cohort analysis.","authors":"N Hamajima,&nbsp;K Aoki","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The future trend in male lung cancer mortality in Japan was predicted by using a simulation model. The model was based on the age-specific death rates from lung cancer in males by birth cohort, expressed as Fi(t) = rkSitr-1 exp(-ktr). The parameters in the function were obtained from the mortality data in Vital Statistics (1960-1980). The chi-square test for goodness-of-fit supported the statistical validity and acceptability of the function. Extrapolation of the function provided future age-specific death rates by birth cohort for males in Japan. In this simulation model it was possible to evaluate the effects of preventive strategies and/or therapeutic improvements on lung cancer mortality when five additional parameters were taken into consideration. According to this model, the age-adjusted death rate from lung cancer in Japanese males is predicted to increase linearly until the year 2000 and to level off thereafter. The total number of deaths from lung cancer for all Japanese males is predicted to be 27,000 in 1990 and over 40,000 in 2000. With the establishment of an effective preventive strategy for young generations, the mortality would begin to decrease a few decades later. Improvements in the therapy of lung cancer, if realized, might suppress the future upward mortality trend in Japan to some extent. The above simulation model based on birth cohort analysis should be useful in estimating the impact of developments in prevention and treatment of lung cancer as well as in predicting the future mortality trend.</p>","PeriodicalId":12660,"journal":{"name":"Gan","volume":"75 7","pages":"578-87"},"PeriodicalIF":0.0,"publicationDate":"1984-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17527811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Presence of colony-promoting activity-responsive cells as a separate compartment of granulocytic cell lineage in mice. 存在的集落促进活性反应细胞作为粒细胞细胞系的一个单独的室在小鼠。
Gan Pub Date : 1984-07-01
T Kamamoto, H Uchino, M Tsurusawa, K J Mori, H Hara, Y Kitamura
{"title":"Presence of colony-promoting activity-responsive cells as a separate compartment of granulocytic cell lineage in mice.","authors":"T Kamamoto,&nbsp;H Uchino,&nbsp;M Tsurusawa,&nbsp;K J Mori,&nbsp;H Hara,&nbsp;Y Kitamura","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A population of cells that responds to colony-enhancing factor has been reported to constitute the most immature subpopulation in the compartment of granulocyte/macrophage progenitors (GM-CFC). A similar colony-promoting activity (CPA) was found in the supernatant of long-term cultures of murine bone marrow cells. Here, some characteristics of the cells responsive to CPA were studied. The CPA-responsive cells in the spleen and bone marrow of W/WV mice were as numerous as in +/+ litter-mates. The concentration of CPA-responsive cells was independent of those of other cell populations, namely pluripotent stem cells (CFU-S), pluripotent precursor cells in vitro and GM-CFC in the spleen and bone marrow. Seeding efficiency in the spleen of irradiated mice and the cell-cycle state of CPA-responsive cells also differed from those of CFU-S and GM-CFC. Accordingly, the target of CPA appears to constitute a separate compartment in the progenitor populations of granulocytic lineage.</p>","PeriodicalId":12660,"journal":{"name":"Gan","volume":"75 7","pages":"603-9"},"PeriodicalIF":0.0,"publicationDate":"1984-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17527813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Functional analogs of bleomycin: sequence-specific DNA cleavage with porphyrin (Fe) intercalators. 博来霉素的功能类似物:用卟啉(Fe)插入物进行序列特异性DNA切割。
Gan Pub Date : 1984-07-01
Y Hashimoto, H Iijima, K Shudo
{"title":"Functional analogs of bleomycin: sequence-specific DNA cleavage with porphyrin (Fe) intercalators.","authors":"Y Hashimoto,&nbsp;H Iijima,&nbsp;K Shudo","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Synthetic porphyrin (Fe) intercalators cleave DNA at guanine-cytosine and guanine-thymine sequences. The specificity for cleavage of base sequences of DNA is quite similar to that of bleomycin.</p>","PeriodicalId":12660,"journal":{"name":"Gan","volume":"75 7","pages":"567-70"},"PeriodicalIF":0.0,"publicationDate":"1984-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17269993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
首页 上一页 下一页 尾页
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整，为了账户安全，请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
请完成安全验证×
相关产品
×
本文献相关产品
联系我们：info@booksci.cn Book学术提供免费学术资源搜索服务，方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号：481959085
Book学术官方微信