Gan Pub Date : 1984-06-01

K Hayashi, R Makino, T Sugimura

引用次数: 0

Gan Pub Date : 1984-06-01

T Shirai, Y Kurata, S Fukushima, N Ito

引用次数: 0

Distribution of blood group antigens and CA 19-9 in gastric cancers and non-neoplastic gastric mucosa. 血型抗原及ca19 -9在胃癌及非肿瘤性胃粘膜中的分布。

Gan Pub Date : 1984-06-01

S Hirohashi, Y Shimosato, Y Ino, Y Tome, M Watanabe, T Hirota, M Itabashi

引用次数: 0

Induction of forestomach hyperplasia by crude butylated hydroxyanisole, a mixture of 3-tert and 2-tert isomers, in Syrian golden hamsters is due to 3-tert-butylated hydroxyanisole. 叙利亚金仓鼠的前胃增生是由3-叔丁基羟基异构体(3-叔丁基羟基异构体和2-叔丁基羟基异构体的混合物)引起的。

Gan Pub Date : 1984-06-01

N Ito, M Hirose, Y Kurata, E Ikawa, Y Mera, S Fukushima

引用次数: 0

Effects of sodium chloride, saccharin, phenobarbital and aspirin on gastric carcinogenesis in rats after initiation with N-methyl-N'-nitro-N-nitrosoguanidine. 氯化钠、糖精、苯巴比妥和阿司匹林对n -甲基-n '-硝基-n -亚硝基胍引发大鼠胃癌的影响。

Gan Pub Date : 1984-06-01

M Takahashi, T Kokubo, F Furukawa, Y Kurokawa, Y Hayashi

{"title":"Effects of sodium chloride, saccharin, phenobarbital and aspirin on gastric carcinogenesis in rats after initiation with N-methyl-N'-nitro-N-nitrosoguanidine.","authors":"M Takahashi, T Kokubo, F Furukawa, Y Kurokawa, Y Hayashi","doi":"","DOIUrl":"","url":null,"abstract":"Sodium chloride, saccharin sodium, phenobarbital sodium and aspirin were tested for tumor-promoting activity in the glandular stomach of rats after initiation with N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) coupled with administration of a high salt diet. Male outbred Wistar rats were given MNNG in the drinking water (100 mg/liter) for 8 weeks, and during this period they were fed on diet supplemented with 10% sodium chloride. Thereafter, they were divided into 5 groups and fed on the basal diet or one of various diets supplemented with 10% sodium chloride, 5% saccharin, 0.05% phenobarbital or 1% aspirin until the end of the experiment. All animals were killed at the 40th experimental week for necropsy and histological examination. The incidence of adenocarcinoma was increased in the group given sodium chloride following initiation by MNNG and sodium chloride as compared with the group given MNNG and sodium chloride initiation only, but not significantly. However, the incidence of preneoplastic hyperplasia was significantly increased in this group. Saccharin also enhanced the development of adenocarcinomas of the glandular stomach. The results indicated that dietary administration of sodium chloride or saccharin after MNNG tends to promote tumor development. Phenobarbital or aspirin did not enhance tumor development, aspirin in fact rather showing a tendency to decrease the tumor incidence.","PeriodicalId":12660,"journal":{"name":"Gan","volume":"75 6","pages":"494-501"},"PeriodicalIF":0.0,"publicationDate":"1984-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17527803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancement of antiproliferation activity of vincristine and adriamycin by interferon. 干扰素增强长春新碱和阿霉素的抗增殖活性。

Gan Pub Date : 1984-06-01

T Kataoka, F Oh-Hashi, Y Sakurai

{"title":"Enhancement of antiproliferation activity of vincristine and adriamycin by interferon.","authors":"T Kataoka, F Oh-Hashi, Y Sakurai","doi":"","DOIUrl":"","url":null,"abstract":"Vincristine (VCR) combined with interferon (IFN)-beta suppressed H.Ep #2 cell proliferation more than additively, whereas 6-mercaptopurine combined with IFN-beta suppressed it less than additively. Factors associated with the more-than-additive effect were examined. The enhanced antiproliferation was achieved in HeLa cells as well as H.Ep #2 cells but not in Daudi or M-14 cells, indicating cell dependency of the enhancement. This enhancement was not dependent on the IFN species, including IFN-beta, IFN-alpha (leukocyte), and IFN-alpha (lymphoblastoid), although the cells were variably sensitive to these IFN species. In contrast, the enhance antiproliferation was critically dependent on the species of antineoplastic agents, and was selective to VCR and adriamycin among those tested under the present experimental conditions. The sequential exposure of H.Ep #2 cells to IFN and VCR induced the enhancement but exposure to VCR followed by IFN did not, suggesting that IFN-induced cell modification made the cells more sensitive to VCR. Either IFN or VCR was successfully replaced by colchicine, an antimicrotubule agent, but not by cytochalasin D, an antimicrofilament agent, suggesting the involvement of microtubule modification in the enhanced antiproliferation observed with IFN and VCR.","PeriodicalId":12660,"journal":{"name":"Gan","volume":"75 6","pages":"548-56"},"PeriodicalIF":0.0,"publicationDate":"1984-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17597071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tumorigenicity of a rabbit lymphoid cell line transformed by human T-cell leukemia virus. 人t细胞白血病病毒转化兔淋巴样细胞系的致瘤性。

Gan Pub Date : 1984-06-01

I Miyoshi, I Kubonishi, S Yoshimoto, Y Ohtsuki, T Akagi

引用次数: 0

Monoclonal antibody reactive with the sialyl-sugar residue of a high molecular weight glycoprotein in sera of cancer patients. 单克隆抗体与肿瘤患者血清中一种高分子量糖蛋白的唾液糖残基反应。

Gan Pub Date : 1984-06-01

S Hirohashi, M Watanabe, Y Shimosato, T Sekine

引用次数: 0

Cytokeratin polypeptide in cultured human squamous cell carcinoma as a possible marker for keratinization. 细胞角蛋白多肽在培养的人鳞状细胞癌中作为角化的可能标志。

Gan Pub Date : 1984-06-01

M Koike, A Komori

{"title":"Cytokeratin polypeptide in cultured human squamous cell carcinoma as a possible marker for keratinization.","authors":"M Koike, A Komori","doi":"","DOIUrl":"","url":null,"abstract":"A human cancer cell line (ZK-1) has been established from a well-differentiated squamous cell carcinoma of the tongue. The cytokeratin polypeptides pattern of ZK-1 cells consists of four major polypeptides with molecular weights between 46 and 58 kilodaltons (kd). Antibodies raised against the purified 58 kd cytokeratin filament from cultured ZK-1 cells were shown to be specific by one- and two-dimensional polyacrylamide gel electrophoresis, immunofluorescence, and immunoelectron microscopy. Immunoprecipitation studies showed that the antibody reacted mainly with the 58 kd cytokeratin. The distribution of the 58 kd cytokeratin in both sparse and confluent cultures was analyzed by the indirect immunofluorescence technique. In both cases, it appeared that fibrillar arrays extended throughout the cytoplasm running over the nucleus and toward cell-to-cell boundaries. Thick bundles of filaments were seen in confluent cultures, especially in large and flattened keratinized cells. Characteristically, reactions were also seen in the intercellular boundaries, appearing as \"dots.\" Electron microscopy using immunoperoxidase techniques indicated that the 58 kd cytokeratin was localized in tonofilaments, tonofilaments attached to desmosomes, desmosomal plaques and membrane-coating granules.","PeriodicalId":12660,"journal":{"name":"Gan","volume":"75 6","pages":"525-33"},"PeriodicalIF":0.0,"publicationDate":"1984-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17269992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cancer-associated alteration of beta-glucuronidase in human lung cancer: elevated activity and increased phosphorylation. 人肺癌中β -葡萄糖醛酸酶的癌症相关改变:活性升高和磷酸化增加。

Gan Pub Date : 1984-06-01

M Fujita, N Taniguchi, A Makita, K Oikawa

{"title":"Cancer-associated alteration of beta-glucuronidase in human lung cancer: elevated activity and increased phosphorylation.","authors":"M Fujita, N Taniguchi, A Makita, K Oikawa","doi":"","DOIUrl":"","url":null,"abstract":"beta-Glucuronidase from human lung neoplasms of various histological types and from uninvolved tissues was studied. A significant elevation of beta-glucuronidase activity was observed in adenocarcinoma and squamous cell carcinoma of the lung as compared with the corresponding uninvolved tissues (P less than 0.01). Saccharo-1,4-lactone, a strong inhibitor of the enzyme, exhibited a substantially greater stabilizing effect on the adenocarcinoma enzyme than on the other enzymes. However, removal of the carbohydrate moiety from the adenocarcinoma enzyme by treatment with endo-beta-N-acetylglucosamidase H (endoglycosidase H) brought about a decrease in the stabilizing effect. Tumor beta-glucuronidase showed considerable negative charge heterogeneity in the pI range from 4.2 to 6.2 in isoelectric focusing on polyacrylamide gel. Upon treatment with exogenous alkaline phosphatase or endoglycosidase H, the heterogenous variant forms of the tumor enzyme appeared to partly or completely lose their negative charge and to be converted into forms similar to those of the normal lung enzyme. These data strongly suggest that the variants are highly phosphorylated on the oligosaccharide chains of the enzyme. An experiment on the labelling of beta-glucuronidase with [32P]-phosphoric acid provided further evidence that the acidic variants found in lung cancers are extensively phosphorylated forms of the enzyme.","PeriodicalId":12660,"journal":{"name":"Gan","volume":"75 6","pages":"508-17"},"PeriodicalIF":0.0,"publicationDate":"1984-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17492253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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