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Early cellular responses in the peritoneal cavity of mice to antitumor immunomodulators. 小鼠腹腔对抗肿瘤免疫调节剂的早期细胞反应。
Gan Pub Date : 1984-04-01
K Morikawa, Y Kikuchi, S Abe, M Yamazaki, D Mizuno
{"title":"Early cellular responses in the peritoneal cavity of mice to antitumor immunomodulators.","authors":"K Morikawa,&nbsp;Y Kikuchi,&nbsp;S Abe,&nbsp;M Yamazaki,&nbsp;D Mizuno","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The early cellular responses to antitumor immunomodulators and conventional inducers, especially the polymorphonuclear leukocyte (PMN) responses, were examined in the peritoneal cavity of mice to investigate their effect on primary defense mechanisms. Immunomodulators were classified into 5 groups in terms of PMN response on the basis of its duration (declining or persistent) and extent (high or low induction): 1) TAK (beta-1,3-glucan)-type (high, persistent), 2) lentinan-type (high, declining), 3) yeast mannan-type (low, declining), 4) LPS (lipopolysaccharide)-type (low, persistent), 5) others (no effect). Since the general PMN response is of the declining type, the persistence of PMN with TAK- and LPS-type immunomodulators is a characteristic of the PMN-inducing activity. With respect to the extent, TAK- and lentinan-type immunomodulators induced larger numbers of PMN and macrophages than conventional inducers. These results suggest that some types of immunomodulators have effects on the early host-defense mechanism. From the viewpoint of the general self-defense mechanism we also compared these PMN responses with those to bacteria and to tumor inoculation, and the properties of substances inducing high PMN response, i.e., those with the quality of \"foreignness,\" are discussed.</p>","PeriodicalId":12660,"journal":{"name":"Gan","volume":"75 4","pages":"370-8"},"PeriodicalIF":0.0,"publicationDate":"1984-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17787940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Application of a Phaseolus vulgaris erythroagglutinating lectin agarose column for the specific detection of human hepatoma gamma-glutamyl transpeptidase in serum. 菜豆红凝集素琼脂糖柱在人肝癌血清γ -谷氨酰转肽酶特异性检测中的应用。
Gan Pub Date : 1984-04-01
A Hitoi, K Yamashita, J Ohkawa, A Kobata
{"title":"Application of a Phaseolus vulgaris erythroagglutinating lectin agarose column for the specific detection of human hepatoma gamma-glutamyl transpeptidase in serum.","authors":"A Hitoi,&nbsp;K Yamashita,&nbsp;J Ohkawa,&nbsp;A Kobata","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>By making use of the structural change of the sugar chains of liver gamma-glutamyl transpeptidase associated with malignant transformation, a new method has been developed to distinguish human serum gamma-glutamyl transpeptidase associated with primary hepatoma from that of non-hepatoma patients. In principle, the method consists of affinity chromatography of the desialylated serum enzyme on a Phaseolus vulgaris erythroagglutinating lectin agarose column.</p>","PeriodicalId":12660,"journal":{"name":"Gan","volume":"75 4","pages":"301-4"},"PeriodicalIF":0.0,"publicationDate":"1984-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17211755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Immunological relatedness of a murine mammary tumor-associated antigen and human breast cancer. 小鼠乳腺肿瘤相关抗原与人乳腺癌的免疫学相关性。
Gan Pub Date : 1984-04-01
J Chattopadhyay, U Chattopadhyay, J R Chowdhury
{"title":"Immunological relatedness of a murine mammary tumor-associated antigen and human breast cancer.","authors":"J Chattopadhyay,&nbsp;U Chattopadhyay,&nbsp;J R Chowdhury","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Immunological relatedness of a non-virion, murine mammary tumor-associated antigen (MTAA) and human breast cancer was demonstrated in this study. The MTAA was isolated from spontaneous mammary tumors (MMT) of C3H/J mice. An anti-serum specific for the MTAA was prepared by exhaustively absorbing the rabbit anti-serum to the MMT extract with tissue pellets of the pooled organs of tumor-free female mice. The observed cross-reaction of human malignant breast tumor (MBT) tissues with the absorbed antiserum further absorbed with normal human breast tissues suggested the presence of the MTAA or a related antigen in the MBT. No MTAA reactivity was found in human benign breast tumor. Circulating antibodies against the MTAA were recorded in all of 14 preoperative patients with MBT. Up to 18 months following surgical removal of the MBT, antibodies could be detected in the sera, though the reactivity was significantly lower than that of the sera of preoperative patients, and eventually disappeared. The antibodies were absent in patients with benign breast tumor and other neoplastic diseases, except in one patient with cancer of the cervix, and in healthy women.</p>","PeriodicalId":12660,"journal":{"name":"Gan","volume":"75 4","pages":"342-8"},"PeriodicalIF":0.0,"publicationDate":"1984-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17391220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Binding of 125I-labeled human interferon to cell lines with low sensitivity to interferon. 125i标记的人干扰素与干扰素低敏感性细胞系的结合。
Gan Pub Date : 1984-04-01
A Fuse, H Ashino-Fuse, T Kuwata
{"title":"Binding of 125I-labeled human interferon to cell lines with low sensitivity to interferon.","authors":"A Fuse,&nbsp;H Ashino-Fuse,&nbsp;T Kuwata","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Iodinated human interferon alpha A (HuIFN-alpha A) was found to bind specifically to IFN-sensitive RSa cells: the dissociation constant was 8.7 X 10(-10)M and the estimated number of receptor sites per cell was 1110. Seven cell lines that were either insensitive or of low sensitivity to the antiproliferative effect of IFN were found to contain a much smaller (3- to 4-fold) number of receptor sites, but the dissociation constants of these binding sites were of the same order of magnitude as those of receptors on RSa cells. Under the present experimental conditions, cell resistance to the antiproliferative effect of IFN is closely associated with the number of receptor sites.</p>","PeriodicalId":12660,"journal":{"name":"Gan","volume":"75 4","pages":"379-84"},"PeriodicalIF":0.0,"publicationDate":"1984-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17391222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Lack of promotion effect of phenobarbital on pulmonary tumorigenesis in ddy mice initiated by transplacental exposure to 1-ethyl-1-nitrosourea. 苯巴比妥对经胎盘暴露于1-乙基-1-亚硝基脲引起的小鼠肺肿瘤发生缺乏促进作用。
Gan Pub Date : 1984-04-01
E Tsuchiya, H F Concetti, H Sugano, T Kitagawa
{"title":"Lack of promotion effect of phenobarbital on pulmonary tumorigenesis in ddy mice initiated by transplacental exposure to 1-ethyl-1-nitrosourea.","authors":"E Tsuchiya,&nbsp;H F Concetti,&nbsp;H Sugano,&nbsp;T Kitagawa","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The promoting effect of phenobarbital (PB) on lung tumorigenesis in mice was examined. Pregnant ddy female mice were injected ip with a single dose of 50 mg/kg 1-ethyl-1-nitrosourea at the 16th day of gestation. Offspring, 160 in total, were divided into two groups: one group of animals was fed a diet containing 0.05% PB continuously and the other was kept on the basal diet. All the animals were killed at 6 months of age and the number, size and histology of pulmonary tumors were examined. There were over 10 pulmonary tumors per animal on average, measuring from 0.5 to 10.2 mm in size, and the histology varied from grade I to III in atypism. A comparative study revealed no difference between PB-treated and control mice in any aspect of the tumorigenesis. It was concluded that PB does not have promoting effect on pulmonary tumorigenesis in this system.</p>","PeriodicalId":12660,"journal":{"name":"Gan","volume":"75 4","pages":"305-10"},"PeriodicalIF":0.0,"publicationDate":"1984-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17788050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Blood group H(O) antigen in normal, dysplastic and carcinomatous esophageal epithelium. 正常、发育不良和癌性食管上皮中的血型H(O)抗原。
Gan Pub Date : 1984-03-01
E Sato, K Maruta, S Yonezawa, T Nakamura
{"title":"Blood group H(O) antigen in normal, dysplastic and carcinomatous esophageal epithelium.","authors":"E Sato,&nbsp;K Maruta,&nbsp;S Yonezawa,&nbsp;T Nakamura","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Variation of the binding Ulex europaeus agglutinin-I (Ulex-I), a lectin specific for blood group H(O) substance, was examined in normal, dysplastic and cancerous esophageal epithelium from 43 instances of carcinoma by means of the lectin- antilectin peroxidase-antiperoxidase method. The normal epithelium showed strong staining at the cell border, although the basal cells were entirely negative. In mild and moderate dysplasia, staining was negative, while in some cases of severe dysplasia positive staining was found in the basal cells. Most of the basal cells in carcinoma in situ revealed granular or diffuse positive staining in the cytoplasm. The results indicate that the loss of blood group H antigen occurs during carcinogenesis of the esophageal mucosa. Thus, Ulex-I may be a useful marker of cytoplasmic differentiation of the esophagus.</p>","PeriodicalId":12660,"journal":{"name":"Gan","volume":"75 3","pages":"223-9"},"PeriodicalIF":0.0,"publicationDate":"1984-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17433225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Immunopotentiation by a new antitumor polysaccharide, DMG, a degraded D-manno-D-glucan from Microellobosporia grisea culture fluid. 一种新的抗肿瘤多糖DMG的免疫增强作用，DMG是一种从灰孢微丝孢菌培养液中降解的d -甘露糖- d -葡聚糖。
Gan Pub Date : 1984-03-01
H Nakajima, Y Kita, T Takashi, M Akasaki, F Yamaguchi, S Ozawa, W Tsukada, S Abe, D Mizuno
{"title":"Immunopotentiation by a new antitumor polysaccharide, DMG, a degraded D-manno-D-glucan from Microellobosporia grisea culture fluid.","authors":"H Nakajima,&nbsp;Y Kita,&nbsp;T Takashi,&nbsp;M Akasaki,&nbsp;F Yamaguchi,&nbsp;S Ozawa,&nbsp;W Tsukada,&nbsp;S Abe,&nbsp;D Mizuno","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The immunopharmacological behavior of DMG, an antitumor polysaccharide, was studied in mice. DMG administered ip or sc stimulated peritoneal macrophages to produce high levels of interleukin-1 activity, which can amplify successive immune responses. DMG dose-dependently and schedule-dependently increased the cellular immune response against allogeneic tumor cells and the humoral immune response to sheep erythrocytes. DMG also enhanced nonspecific antitumor effector functions, such as natural killer activity of spleen and peritoneal cells, and the cytostatic activity of peritoneal macrophages. Peritoneal macrophages activated by ip or sc injection of DMG exhibited high cytostatic activity, especially after exposure in vitro to lymphokine supernatants containing macrophage activation factor. Moreover, granulocyte/macrophage colony-stimulating activity in the serum increased 2-10 hr after DMG administration. Thus, DMG potentiated antigen-specific immunological functions and nonspecific functions of host defense systems against cancer both qualitatively and quantitatively.</p>","PeriodicalId":12660,"journal":{"name":"Gan","volume":"75 3","pages":"260-8"},"PeriodicalIF":0.0,"publicationDate":"1984-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17486828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Distinctive sensitivity of some T-leukemia cell lines to L-asparaginase. 某些t -白血病细胞系对l -天冬酰胺酶的特殊敏感性。
Gan Pub Date : 1984-03-01
T Koishi, J Minowada, E S Henderson, T Ohnuma
{"title":"Distinctive sensitivity of some T-leukemia cell lines to L-asparaginase.","authors":"T Koishi,&nbsp;J Minowada,&nbsp;E S Henderson,&nbsp;T Ohnuma","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Forty-two human hematopoietic cell lines were assessed for sensitivity to a four-day incubation with L-asparaginase. Eight of 13 T-leukemia cell lines and 1 of 7 non-T, non-B common acute lymphoblastic leukemia cell lines exhibited an ID50 (50% growth inhibition dose) of less than 0.0001 IU/ml. Seven of these sensitive cell lines were further cultured in L-asparagine-free medium and were found not to proliferate. Five T-leukemia cell lines, 6 non-T, non-B common acute lymphoblastic leukemia cell lines, 10 \"abnormal\" B-cell lines, 4 \"normal\" B-cell lines, 3 myeloma cell lines and 5 myeloid leukemia cell lines had ID50 values between 0.1 and 1.0 IU/ml. Twelve of these resistant cell lines were able to proliferate in L-asparagine-depleted medium. The results indicate that some patients with T-cell acute lymphoblastic leukemia might respond to this enzymatic antineoplastic drug, L-asparaginase, at low dose.</p>","PeriodicalId":12660,"journal":{"name":"Gan","volume":"75 3","pages":"275-83"},"PeriodicalIF":0.0,"publicationDate":"1984-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17666024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Pharmacokinetics of human recombinant interferon-beta in monkeys and rabbits. 人重组干扰素- β在猴子和家兔体内的药动学。
Gan Pub Date : 1984-03-01
K Gomi, M Morimoto, A Inoue, H Kobayashi, T Deguchi, T Hara, N Nakamizo
{"title":"Pharmacokinetics of human recombinant interferon-beta in monkeys and rabbits.","authors":"K Gomi,&nbsp;M Morimoto,&nbsp;A Inoue,&nbsp;H Kobayashi,&nbsp;T Deguchi,&nbsp;T Hara,&nbsp;N Nakamizo","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The pharmacokinetics of human recombinant interferon-beta ( ReIFN -beta) was compared with that of natural human interferon-beta (IFN-beta) in monkeys and rabbits after intravenous or intramuscular injection. After intravenous injection of 10(6) units/kg of ReIFN -beta or IFN-beta into monkeys or rabbits, serum levels of both interferons declined biexponentially. No significant differences between ReIFN -beta and IFN-beta were detected in most pharmacokinetic parameters including T1/2-beta, though T1/2-alpha of ReIFN -beta was significantly shorter than that of IFN-beta. These results seemed to be in conflict with the observed difference of stability of the interferons in vitro since ReIFN -beta was less stable than IFN-beta in monkey and rabbit serum. When ReIFN -beta (10(7) units/kg) was injected intramuscularly into monkeys or rabbits, it remained detectable in the serum for 24 hr; an absorption phase and an elimination phase were seen. However, AUC (the area under the serum concentration curve) after the intramuscular injection of ReIFN -beta was about one-half and one-third of that after the intravenous injection in monkeys and rabbits, respectively. ReIFN -beta and IFN-beta (10(6) units/kg) were both detectable in the serum after intramuscular injection into rabbits, but the level of ReIFN -beta was lower than that of IFN-beta. These results indicate that the lack of carbohydrate in ReIFN -beta did not essentially affect the in vivo pharmacokinetics in monkeys and rabbits after intravenous injection, but this was not the case after intramuscular injection.</p>","PeriodicalId":12660,"journal":{"name":"Gan","volume":"75 3","pages":"292-300"},"PeriodicalIF":0.0,"publicationDate":"1984-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17777364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Formation of volatile nitrosamines by drug-nitrite interactions under physiological conditions. 生理条件下药物-亚硝酸盐相互作用下挥发性亚硝胺的形成。
Gan Pub Date : 1984-03-01
A Sakai, T Inoue, A Tanimura
{"title":"Formation of volatile nitrosamines by drug-nitrite interactions under physiological conditions.","authors":"A Sakai,&nbsp;T Inoue,&nbsp;A Tanimura","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Twenty-eight drugs, most of which are tertiary amines, were tested for the formation of volatile nitrosamines by reaction with nitrite under physiological conditions; the drugs (10mM) were incubated with nitrite ( 40mM ) at pH 3.0, 37 degrees for 1 and 4 hr. The volatile nitrosamines formed were determined by gas chromatography-thermal energy analysis. Of the 28 drugs, 24 formed measurable amounts of volatile nitrosamines that are known carcinogens. The yields of nitrosodimethylamine (NDMA) from aminopyrine (55-65%) and minocycline (11%) were higher than that from dimethylamine under the same conditions. This result suggests that there may be a pathway not involving the secondary amine (dimethylamine) as an intermediate in the formation of NDMA from minocycline as well as from aminopyrine, Tolazamide gave rise to nitrosopiperidine ( NPIP ) in addition to nitrosohexamethyleneimine ( NHXI ), formation of which was expected from the chemical structure of tolazamide, and the yield of NPIP (2-7%) was higher than that of NHXI (0.2-1.2%). Ascorbic acid ( 40mM ) was effective in decreasing the formation of nitrosamines from drugs by reaction with nitrite, although the blocking effects varied between 88 and 100% depending on the drugs tested or on the nitrosamines formed.</p>","PeriodicalId":12660,"journal":{"name":"Gan","volume":"75 3","pages":"245-52"},"PeriodicalIF":0.0,"publicationDate":"1984-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17777362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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