一种单克隆抗体，定义成人t细胞白血病病毒的核心蛋白p24及其前体。

Gan Pub Date : 1984-07-01

M Fujii, K Sugamura, Y Hinuma

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引用次数: 0

摘要

小鼠杂交瘤细胞系H15产生单克隆抗体，与所有携带ATL病毒(ATLV)的成年t细胞白血病(ATL)病毒(ATLV)的细胞系反应，但对ATLV阴性的细胞系没有反应。H15抗体与携带atlv的细胞表面的结合被抗atlv阳性的人血清特异性阻断。放射免疫沉淀分析显示，H15抗体检测到的抗原为atv的核心蛋白p24。利用H15抗体进行的脉冲追踪实验鉴定出一种蛋白质p53，它可能是p24的前体。

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A monoclonal antibody that defines p24, a core protein of adult T-cell leukemia virus, and its precursor.

A mouse hybridoma cell line, H15, produced monoclonal antibody reacting with all the adult T-cell leukemia (ATL) virus (ATLV)-bearing cell lines but none of the ATLV-negative cell lines tested. Binding of H15 antibody to ATLV-bearing cell surfaces was specifically blocked by anti-ATLV positive human sera. Radioimmunoprecipitation analyses revealed that the antigen detected by H15 antibody was p24, a core protein of ATLV. Pulse-chase experiments using H15 antibody led to the identification of a protein, p53, which could be a precursor of p24.

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Gan

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