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Interleukin-1 restores the impaired cytotoxic T lymphocyte generation in beige mutant mouse. 白细胞介素-1可恢复米色突变小鼠受损的细胞毒性T淋巴细胞生成。
Gan Pub Date : 1984-09-01
K Okuno, K Takatsu, Y Takahama, Y Kitamura, T Hamaoka
{"title":"Interleukin-1 restores the impaired cytotoxic T lymphocyte generation in beige mutant mouse.","authors":"K Okuno,&nbsp;K Takatsu,&nbsp;Y Takahama,&nbsp;Y Kitamura,&nbsp;T Hamaoka","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Spleen cells from C57BL/6 beige mouse showed significantly lower cytotoxic T lymphocyte (CTL) generation in vitro against allogeneic target cells as compared with spleen cells from the wild type, whereas the heterozygous littermate showed a response similar to that of the wild type. In contrast, the responsiveness of beige spleen cells in the mixed lymphocyte reaction against allogeneic stimulator cells was in the normal range, suggesting that beige spleen cells recognize allogeneic stimulator cells to the same extent as spleen cells from normal mouse, resulting in a significant proliferation. The addition of interleukin 1 (IL-1)-containing supernatant from lipopolysaccharide-stimulated J774.1 cells to the culture of spleen cells from beige mouse stimulated with allogeneic cells restored the impaired CTL generation in a dose-dependent manner. The molecules responsible for restoration of the impaired CTL response co-migrated with IL-1 on gel filtration. The addition of purified interleukin 2(IL-2) also augmented the induction of CTL from beige spleen cells. However, the magnitude of augmentation by IL-2 was appreciably lower than that of augmentation by IL-1. These results suggest that the role of IL-1 in the induction of CTL is not only to provide a signal for activated amplifier T cells to release IL-2, but also to magnify otherwise low responsiveness of CTL-precursors and/or CTL-helpers. Moreover, intraperitoneal injection of IL-1 without allo-antigenic stimulation was able to restore the in vitro CTL responsitivity to allo-antigen but not the natural killer cell activity, indicating that IL-1 has a therapeutic potential in vivo for preferentially correcting impaired CTL generation associated with beige mutation.</p>","PeriodicalId":12660,"journal":{"name":"Gan","volume":"75 9","pages":"824-32"},"PeriodicalIF":0.0,"publicationDate":"1984-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17303146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Amplification of the c-myc oncogene in human stomach cancers. 人胃癌c-myc癌基因的扩增。
Gan Pub Date : 1984-09-01
F Nakasato, H Sakamoto, M Mori, K Hayashi, Y Shimosato, M Nishi, S Takao, K Nakatani, M Terada, T Sugimura
{"title":"Amplification of the c-myc oncogene in human stomach cancers.","authors":"F Nakasato,&nbsp;H Sakamoto,&nbsp;M Mori,&nbsp;K Hayashi,&nbsp;Y Shimosato,&nbsp;M Nishi,&nbsp;S Takao,&nbsp;K Nakatani,&nbsp;M Terada,&nbsp;T Sugimura","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Amplified c-myc oncogene was found in the DNAs of 2 of 11 human stomach cancers transplanted into nude mice; the amplification was 8- to 10-fold in one tumor and 13- to 15-fold in the other. Both tumors in which the c-myc oncogene was amplified were poorly differentiated adenocarcinomas, but there was no clear-cut correlation between the histological types or growth rates of the tumors and amplification of the c-myc oncogene. No amplification of the c-myc gene was detected in DNAs from 4 cultured stomach cancer cell lines, 19 primary stomach cancers or 11 metastases to lymph nodes from human stomach cancers.</p>","PeriodicalId":12660,"journal":{"name":"Gan","volume":"75 9","pages":"737-42"},"PeriodicalIF":0.0,"publicationDate":"1984-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17557839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Identification of a protein (p40x) encoded by a unique sequence pX of human T-cell leukemia virus type I. 人I型t细胞白血病病毒独特序列pX编码蛋白p40x的鉴定
Gan Pub Date : 1984-09-01
T Kiyokawa, M Seiki, K Imagawa, F Shimizu, M Yoshida
{"title":"Identification of a protein (p40x) encoded by a unique sequence pX of human T-cell leukemia virus type I.","authors":"T Kiyokawa,&nbsp;M Seiki,&nbsp;K Imagawa,&nbsp;F Shimizu,&nbsp;M Yoshida","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A protein p40x was identified as a product encoded by frame IV in the pX region of human T-cell leukemia virus type I. Sera from patients with adult T-cell leukemia contained antibodies against p40x, indicating its expression in vivo. The occurrence of splicing to form pX mRNA is proposed and the possible significance of p40x is discussed.</p>","PeriodicalId":12660,"journal":{"name":"Gan","volume":"75 9","pages":"747-51"},"PeriodicalIF":0.0,"publicationDate":"1984-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17161441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Biological activity of a fluorescent 12-O-tetradecanoyl-phorbol-13-acetate derivative. 12- o -十四烷基苯酚-13-乙酸酯荧光衍生物的生物活性。
Gan Pub Date : 1984-09-01
E Honikman-Leban, Y Moulé
{"title":"Biological activity of a fluorescent 12-O-tetradecanoyl-phorbol-13-acetate derivative.","authors":"E Honikman-Leban,&nbsp;Y Moulé","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A newly synthesized fluorescent TPA derivative, O-(N-dansylamino-3-tetradecanoyl)-12,O-acetyl-13-phorbol (Dansyl-TPA), inhibited metabolic cooperation of Chinese hamster V79 cells in culture and also the specific binding of [3H]phorbol dibutyrate to V79 cells. Dansyl-TPA should be a valuable tool for studying the cellular targets of TPA.</p>","PeriodicalId":12660,"journal":{"name":"Gan","volume":"75 9","pages":"729-31"},"PeriodicalIF":0.0,"publicationDate":"1984-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17650730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Clonal origin of spontaneous multiple mammary tumors in mice with cellular mosaicism. 细胞嵌合小鼠自发性多发性乳腺肿瘤的克隆起源。
Gan Pub Date : 1984-09-01
K Tanaka, A Ootsuyama, H Tanooka
{"title":"Clonal origin of spontaneous multiple mammary tumors in mice with cellular mosaicism.","authors":"K Tanaka,&nbsp;A Ootsuyama,&nbsp;H Tanooka","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The clonal origin of spontaneous multiple mammary tumors in mice was examined. For this purpose, hybrid female mice (Pgk-1b/Pgk-1a), F1[SHN(Pgk-1b) X C3H/He (Pgk-1a)], with X-chromosome inactivation mosaicism with regard to the phosphoglycerate kinase (PGK)-1 isozyme together with a high incidence of spontaneous mammary tumors were constructed. Thirty-seven of 45 mammary tumors (82%) in mosaic mice had a single phenotype of PGK, indicating monoclonal origin. The multiple mammary tumors formed in these mice varied in PGK type, indicating independent cellular origins.</p>","PeriodicalId":12660,"journal":{"name":"Gan","volume":"75 9","pages":"792-7"},"PeriodicalIF":0.0,"publicationDate":"1984-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17556982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Integration of v-rasH does not necessarily transform an immortalized murine cell line. v-rasH的整合并不一定能转化永生化的小鼠细胞系。
Gan Pub Date : 1984-09-01
Y Tsunokawa, H Esumi, M S Sasaki, M Mori, H Sakamoto, M Terada, T Sugimura
{"title":"Integration of v-rasH does not necessarily transform an immortalized murine cell line.","authors":"Y Tsunokawa,&nbsp;H Esumi,&nbsp;M S Sasaki,&nbsp;M Mori,&nbsp;H Sakamoto,&nbsp;M Terada,&nbsp;T Sugimura","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Plasmids containing v-rasH and Ecogpt were constructed, and used to transfect two established cell lines of mouse origin, NIH3T3 cells and m5S cells. After transfection, most NIH3T3 cells, which are resistant to mycophenolic acid, showed phenotypes characteristic of neoplastic transformation, whereas no mycophenolic acid-resistant m5S cells showed these phenotypes; integration of functionally intact v-rasH in immortalized murine cells is not sufficient for neoplastic transformation in m5S cells. The resistance to the transformation was probably due to a lower level of the v-rasH gene transcripts in m5S cells.</p>","PeriodicalId":12660,"journal":{"name":"Gan","volume":"75 9","pages":"732-6"},"PeriodicalIF":0.0,"publicationDate":"1984-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17557838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Binding of bile acids with rat colon and resultant perturbation of membrane organization as studied by uptake measurement and 31P nuclear magnetic resonance spectroscopy. 胆汁酸与大鼠结肠的结合及由此引起的膜组织扰动采用摄取测量和31P核磁共振波谱法研究。
Gan Pub Date : 1984-09-01
Y Sugimoto, H Saitô, R Tabeta, M Kodama, C Nagata, M Itabashi, T Hirota, S Toyoshima
{"title":"Binding of bile acids with rat colon and resultant perturbation of membrane organization as studied by uptake measurement and 31P nuclear magnetic resonance spectroscopy.","authors":"Y Sugimoto,&nbsp;H Saitô,&nbsp;R Tabeta,&nbsp;M Kodama,&nbsp;C Nagata,&nbsp;M Itabashi,&nbsp;T Hirota,&nbsp;S Toyoshima","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The mode of interaction of deoxycholate (DOC) or lithocholate (LC) with F344 rat colon was examined by measurements of uptake, 31P nuclear magnetic resonance (NMR) spectroscopy and observation of morphological changes. DOC as well as LC was taken up by the colon in a nonsaturable manner with respect to concentration and time, up to 30 min. None of several metabolic inhibitors reduced the uptake of the bile acids, nor did pretreatment of colon segments with chloroform-methanol (2:1, (v/v), heat or trypsin. Further, the bile acids were not transported by the colon against concentration gradients, and they were bound to both the mucosa and serosa equally. From these findings, it is concluded that the bile acids are transported in a passive manner, and no specific receptor for them is contained in colonic mucosa. The uptake of the bile acids by the colon varied with temperature and was related to the fluidity of the colonic membranes. The extent of uptake of dehydrocholate and taurocholate, which do not induce ornithine decarboxylase (ODC) activity, was almost the same as that of LC. The 31P NMR spectra of the colonic mucosal cells indicated that the proportion of the bilayer structure is increased by 0.5 mM DOC. Among a variety of bile acids examined, the extent of membrane alteration was in parallel with the extent of ODC induction. Treatment of the colonic mucosa with 0.5 mM DOC caused marked degeneration of the surface but not the deeper layers of the mucosa. Thus, physiological concentrations of bile acids influence the membrane organization of the colonic mucosa in a nonspecific manner that is possibly related to the tumor-promoting activity.</p>","PeriodicalId":12660,"journal":{"name":"Gan","volume":"75 9","pages":"798-808"},"PeriodicalIF":0.0,"publicationDate":"1984-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17556983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Direct evidence for the expression of integrated hepatitis B virus DNA in human hepatoma tissue. 乙肝病毒DNA在人肝癌组织中表达的直接证据。
Gan Pub Date : 1984-09-01
K Yaginuma, H Kobayashi, E Yoshida, M Kobayashi, K Koike
{"title":"Direct evidence for the expression of integrated hepatitis B virus DNA in human hepatoma tissue.","authors":"K Yaginuma,&nbsp;H Kobayashi,&nbsp;E Yoshida,&nbsp;M Kobayashi,&nbsp;K Koike","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The intracellular structure and expression of integrated hepatitis B virus (HBV) DNA in the hepatoma tissue #1707 obtained from an 11-year-old boy were studied. It was found that most of the HBV genome was present in the #1707 DNA. Virus-specific 21S mRNA was also demonstrated by blot hybridization. This is the first direct evidence for the expression of the integrated viral genome in human hepatoma tissue.</p>","PeriodicalId":12660,"journal":{"name":"Gan","volume":"75 9","pages":"743-6"},"PeriodicalIF":0.0,"publicationDate":"1984-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17161440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Ribonucleotide reductase and thymidine kinase activities in various cultured cell lines derived from hematologic malignancies. 核糖核苷酸还原酶和胸苷激酶活性的各种培养细胞系源自血液恶性肿瘤。
Gan Pub Date : 1984-09-01
E Takeda, M Hirose, Y Kuroda, T Ninomiya, K Toshima, T Watanabe, M Ito, E Naito, M Miyao
{"title":"Ribonucleotide reductase and thymidine kinase activities in various cultured cell lines derived from hematologic malignancies.","authors":"E Takeda,&nbsp;M Hirose,&nbsp;Y Kuroda,&nbsp;T Ninomiya,&nbsp;K Toshima,&nbsp;T Watanabe,&nbsp;M Ito,&nbsp;E Naito,&nbsp;M Miyao","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The activities of ribonucleotide reductase and thymidine kinase, and the thymidine incorporation rate were measured in 16 cultured human hematologic malignant cell lines with different cell proliferation rates. Thymidine kinase activity was significantly higher in myeloid and monocytoid cell lines than in other cell lines, but ribonucleotide reductase activity presented as CDP reductase activity was similar in the different cell lines. The ratio of thymidine kinase to CDP reductase activity was high in monocytoid cell lines. A close correlation was found between the cell proliferation rate and CDP reductase activity, but not thymidine kinase activity or the thymidine incorporation rate. The ratio of thymidine kinase to CDP reductase activity was high in slowly growing cell lines and low in rapidly growing cell lines. These results indicate that in cultured human malignant cells a high potential for proliferation may depend mainly on the de novo pyrimidine pathway of DNA biosynthesis.</p>","PeriodicalId":12660,"journal":{"name":"Gan","volume":"75 9","pages":"816-23"},"PeriodicalIF":0.0,"publicationDate":"1984-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17449188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effects of 12-O-tetradecanoylphorbol-13-acetate on carcinogenesis in the heterotopically transplanted rat urinary bladder. 12- o -十四烷醇-13-醋酸酯对异位移植大鼠膀胱癌变的影响。
Gan Pub Date : 1984-09-01
K Izumi, M Shibata, K Togei, A Akagi, H Otsuka, J B Jacobs, S Ozono, Y Miyata, R Oyasu
{"title":"Effects of 12-O-tetradecanoylphorbol-13-acetate on carcinogenesis in the heterotopically transplanted rat urinary bladder.","authors":"K Izumi,&nbsp;M Shibata,&nbsp;K Togei,&nbsp;A Akagi,&nbsp;H Otsuka,&nbsp;J B Jacobs,&nbsp;S Ozono,&nbsp;Y Miyata,&nbsp;R Oyasu","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>12-O-Tetradecanoylphorbol-13-acetate (TPA), a potent promoter of mouse skin carcinogenesis, was tested for possible tumor-enhancing effects on urinary bladder carcinogenesis using the heterotopically transplanted bladder (HTB) model. Weekly administration of TPA at 1.0 microgram/week to N-methyl-N-nitrosourea-initiated HTBs did not increase tumor incidence, but instead, resulted in a significantly high incidence of nodulopapillary hyperplasia, an early neoplastic lesion, suggesting possible tumor enhancement by TPA. In addition, administration of a high dose of TPA with or without a carcinogen treatment led to the development of numerous finger-like epithelial projections on the luminal surface of the HTBs. Evidence indicates that epithelial projections are formed as a result of proliferation of intermediate cells. Whether these structures evolve into true neoplastic lesions is at present unknown.</p>","PeriodicalId":12660,"journal":{"name":"Gan","volume":"75 9","pages":"756-62"},"PeriodicalIF":0.0,"publicationDate":"1984-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17557840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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