Geomicrobiology Journal最新文献

{"title":"Microbial Induced Calcite Precipitation (MICP) Potential of Ureolytic Bacillus sp. Isolated from the Soil of Eroded Ecosystems for Stabilizing and Improving the Fertility of Eroded Soils","authors":"Somaye Fazelikia, S. A. Abtahi, M. Kargar, Mojtaba Jafarinia","doi":"10.1080/01490451.2023.2211077","DOIUrl":"https://doi.org/10.1080/01490451.2023.2211077","url":null,"abstract":"Abstract The loss of soil from lands due to erosion has a negative effect on ecosystems and food security. Bacillus due to high catabolic capability is an appropriate candidate for application in biocementation process. The aim of this study is isolate and characterize Bacillus sp. with biocementation capability from various ecosystems. The isolates were separated from 400 samples, and characterized by biochemical and molecular methods include the amplification and sequencing analysis of gyrA and 16S rRNA genes. Growth in presence of urea, in different salinity, pH, and temperature, also scanning electron microscope (SEM), X-ray diffraction (XRD), and wind tunnel analysis were applied to determine biocementation ability. A total number of 195 isolates were recovered from environmental samples, of which 25 isolates (12.82%) were identified as urease-positive Bacillus which belonged to 10 species consisting of Bacillus subtilis five strains (20%), Bacillus vallismortis and Bacillus seohaeanensis four strains (16%) each, Bacillus mobilis, Bacillus pseudofirmus, Bacillus cohnii, Bacillus cereus, Bacillus alkalinitrilicus two strains (8%) each, and Bacillus sphaericus and Bacillus megaterium one strain (4%) each. Moreover, 15 urease-positive isolates (7.7%) belonging to Ralostenia, Actinomycete, and Halomonas genera were identified. Optimum conditions for microbial induced calcite precipitation (MICP) by isolates are 30 °C, pH 9, and 6% salinity. The highest rate of calcium carbonate formation and urease activity recorded in B. subtilis with 24.15 mg/mL of calcium carbonate and 4.40 × 103 unit/L of urease, followed by B. mobilis and B. alkalinitrilicus with 22.85 mg/mL of calcium carbonate and 3.93 × 103 unit/L of urease. After MICP the lowest soil loss ratio at a flow rate of 90 km/h, was observed in B. subtilis 100-fold reduction, followed by B. seohaeanensis, B. cereus, B. vallismortis, with 90, 85, 80-folds reduction, respectively. Results indicate that the diversity of Bacillus sp. offers the potential ability for adaptation to harsh and untapped environments, also showed that the use of MICP on the soil surface can have a very significant role in reducing soil losses due to wind erosion.","PeriodicalId":12647,"journal":{"name":"Geomicrobiology Journal","volume":"40 1","pages":"569 - 581"},"PeriodicalIF":2.3,"publicationDate":"2023-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45117426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation of Inorganic Phosphorus-Solubilizing Bacteria from the Rhizosphere of Festuca arundinacea Schreb.","authors":"Yinping Liang, Zhiyu Xu, Qingfang Xu, Xiang Zhao, Shuaishuai Niu, Xiongyu Yin","doi":"10.1080/01490451.2023.2208096","DOIUrl":"https://doi.org/10.1080/01490451.2023.2208096","url":null,"abstract":"Abstract Inorganic phosphorus-solubilizing bacteria promote plant growth. However, different strains have different effects. The objective of this study was to screen efficient inorganic phosphorus-solubilizing bacteria (iPSB) from the rhizosphere of Festuca arundinacea Schreb. using Pikovskaya (PVK) medium. The results showed that 43 iPSB isolates were screened by clear halo zone production on PVK medium. Among them, the phosphate solubilization index (PSI) on the PVK medium of 31 iPSBs was calculated to be more than 1.5 at 28 °C in 7 days and ranged from 1.52 to 2.63. The best 31 iPSB isolates were selected for further study, including molecular identification using the 16S rRNA gene, testing of their P solubilization capacity in PVK broth medium, and the detection of catalase activity and organic acid and siderophore production. Among the 31 isolates, the phosphate solubilization of 8 isolates ranged from 203.96 to 412.22 µg/mL, and the value was significantly larger than that of the PVK broth medium without any bacterial isolates. These isolates were identified and belonged to Acinetobacter calcoaceticus, Buttiauxella sp., and Erwinia piriflorinigrans by sequence analysis of the 16S rRNA gene. These iPSB bacterial isolates enrich plant growth-promoting rhizobacteria and can promote the development of sustainable agriculture as phosphate fertilizer synergists.","PeriodicalId":12647,"journal":{"name":"Geomicrobiology Journal","volume":"40 1","pages":"538 - 546"},"PeriodicalIF":2.3,"publicationDate":"2023-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48863286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Resistance to Sulfate Attack Under Drying–wetting Cycles of Reactive Magnesia–microbial Cured Electrolytic Manganese Residue","authors":"Jinlong Liu, X. Fang, Chun-miao Shen, Fenghui Hu, Xichen Zhang, Mingming Wang","doi":"10.1080/01490451.2023.2211070","DOIUrl":"https://doi.org/10.1080/01490451.2023.2211070","url":null,"abstract":"Abstract The reactive MgO (r-MgO)-microbial curing technology can not only effectively improve the strength of electrolytic manganese residue (EMR) but also repair heavy metal ions. By conducting unconfined compressive strength (UCS) test, heavy metal leaching test, X-ray diffraction (XRD) analysis, scanning electron microscopy (SEM), and mercury intrusion porosimetry (MIP), the effects of the type and concentration of sulfate on the strength and repair of heavy metals of the cured EMR under drying–wetting (DW) cycles were studied, and the strength and the evolution mechanism of heavy metal remediation is elucidated. The results indicate the following: With the increase in the number of DW cycles, the dry density of the samples in high concentration of MgSO4 first increased slightly and then decreased gradually and became stable. The dry density of the samples in Na2SO4, low concentration of MgSO4, and water generally showed a downward trend. The pH of the soaking solution slightly decreased, and the leaching concentration of heavy metal Mn2+ ions always remained at a low level. The UCS of the samples in MgSO4 first increased slightly and then decreased gradually, and then became stable. The amount of cured products first increased and then decreased, and the size of internal pores first decreased and then increased. The UCS of the samples in Na2SO4 and water gradually decreased and became stable. The amount of cured products gradually decreased, and the size of internal pores gradually increased. The research results provide a theoretical basis for the evaluation of resistance against the sulfate corrosion of cured EMR under DW cycles.","PeriodicalId":12647,"journal":{"name":"Geomicrobiology Journal","volume":"40 1","pages":"547 - 559"},"PeriodicalIF":2.3,"publicationDate":"2023-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45099329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of phosphorus fertilizers and phosphorus-solubilizing rhizosphere bacteria on soil fertility, phosphorus uptake, growth, and yield of sesame (Sesamum indicum L.) cultivated on alluvial soil in dike","authors":"L. Thuc, Lê Thị Mỹ Thu, T. Hữu, Ph. H. Nghi, L. T. Quang, D. T. Xuan, L. Xuan, N. Khuong","doi":"10.1080/01490451.2023.2204860","DOIUrl":"https://doi.org/10.1080/01490451.2023.2204860","url":null,"abstract":"Abstract The low phosphorus use efficiency (PUE) and the high P fertilizer cost are reasons why it is necessary to find sustainable phosphorus (P) sources for sesame, in which bacteria are potential candidates. The aim of this study was to determine a level of the applied P fertilizer and P-solubilizing rhizosphere bacteria (PSRB) for the maximum growth and yield of sesame. A two-factor experiment was conducted in a completely randomized block design. The first factor (i) was the P fertilizer rate, including 0%, 50%, 75%, and 100% P of the recommended fertilizer rate (RFR); the other was (ii) the supplementation of the PSRB, including no supplemented bacteria, a single strain of Enterobacter asburiae ASD-56, ASD-15, or ASD-43, and the mixture of all the three strains, with five replications. The results revealed that fertilizing with 100% P of the RFR enhanced means of the plant height (5.4 cm), the number of capsules (4.4 capsules plant−1), the total P uptake (34.3 mg P pot−1), and the sesame seed yield (16.4 g pot−1) in comparison with the case with no P fertilizer. The supplementation of the E. asburiae ASD-56, ASD-15, and ASD-43 improved values of the soluble P in soil, the total P uptake in plants, and the seed yield at 82.6 mg P kg−1, 73.1 mg P pot−1, and 15.2 g pot−1, respectively, compared with 72.2 mg P kg−1, 45.5 mg P pot−1, and 10.6 g pot−1 in treatment with no supplemented bacteria. Individually or aggregately applying the three E. asburiae ASD-56, ASD-15, and ASD-43 strains not only reduced the average amount of the P fertilizer used by 25–50% P of the RFR but also increased the P uptake in plants by 8.50–36.9% and the average sesame seed yield by 2.94–58.7%, in comparison with those in the treatment fertilized with 100% P of the RFR. The mixture of the PSRB contributed to reducing 50% of the RFR, and increasing the yield by 43.4%.","PeriodicalId":12647,"journal":{"name":"Geomicrobiology Journal","volume":"40 1","pages":"527 - 537"},"PeriodicalIF":2.3,"publicationDate":"2023-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45906361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Review on Soil Solidification and Heavy Metal Stabilization by Microbial-Induced Carbonate Precipitation (MICP) Technology","authors":"Feng C. Xu, Dongxing Wang","doi":"10.1080/01490451.2023.2208113","DOIUrl":"https://doi.org/10.1080/01490451.2023.2208113","url":null,"abstract":"Abstract Microbial-induced carbonate precipitation (MICP) as a novel eco-friendly bioremediation method has been applied tentatively in soil solidification and heavy metal stabilization. In order to deeply study the theoretical knowledge of MICP and its application in environmental engineering, this review summarizes the main mechanisms of MICP, including biosorption and biomineralization. It is proposed innovatively that bacterial cell wall and extracellular polymeric substances (EPS) can provide ions binding sites during the process of biomineral nucleation due to negative charges and functional groups. Engineering properties of soil are regulated, such as increase of unconfined compressive strength (UCS) and shear stress, decrease of permeability and improvement of erosion resistance. Supposing the available CaCO3 content (CCC), UCS can be estimated by two boundary lines with an intersection angle of 2.72°. The great bioremediation capacity of metal cations/anions greatly depends upon carbonate precipitation/coprecipitation. Biotransformation and bioaccumulation of metal poisonousness should be considered as essential mechanisms in bacteria. Finally, according to the authors’ knowledge, current deficiencies and future research directions for the technology were pointed out, which might be beneficial to the optimization, application and generalization of MICP technology.","PeriodicalId":12647,"journal":{"name":"Geomicrobiology Journal","volume":"40 1","pages":"503 - 518"},"PeriodicalIF":2.3,"publicationDate":"2023-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43556112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bio-carbonization of Reactive Magnesia for Sandy Soil Solidification","authors":"Jiancai Yan, Huaxun Wu, Yamin Ding, Jiahua Fan","doi":"10.1080/01490451.2023.2204855","DOIUrl":"https://doi.org/10.1080/01490451.2023.2204855","url":null,"abstract":"Abstract Bio-carbonization of reactive magnesia (r-MgO) is a new technology for sandy soil solidification. In this study, two sets of tests were conducted to investigate the influence of r-MgO contents on the bio-solidification effects of sandy soils, with the analysis of the unconfined compressive strength (UCS), permeability coefficient, sonic time value, and precipitation content. The relationship between r-MgO contents and solidification effects with a single treatment cycle was studied in the first sand solidification test. Then, the second sand solidification test was further conducted until their permeability coefficient reached about 10−6 cm/s to determine the maximum treatment cycle under various r-MgO contents. The results showed that the UCS, permeation resistance, and carbonate precipitation content were positively related to the r-MgO content if the solidification treatment was applied only once, while the sonic time value showed an opposite trend. Moreover, the maximum treatment cycle obtained under various r-MgO contents varied greatly. A high dosage of r-MgO could clearly reduce the maximum number of treatment cycles of the sand column, especially the r-MgO content larger than 15%. Decreased treatment cycle reduced carbonate precipitations in the sand column and decreased the UCS by over 40%. There was a close relationship between UCS and average carbonate precipitation contents for the bio-carbonated sand columns with the only one treatment cycles. However, the UCS of sand columns with multiple treatment cycles varied greatly within a similar average precipitation content. The results of this study lay a solid foundation for applying bio-carbonization of r-MgO in sandy soil solidification.","PeriodicalId":12647,"journal":{"name":"Geomicrobiology Journal","volume":"40 1","pages":"519 - 526"},"PeriodicalIF":2.3,"publicationDate":"2023-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45079513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An Exploratory Research on the Adoption of Different Phosphate-Solubilizing Fungi for Production of Phosphate Biofertilizers","authors":"Laleh Faridian, J. Baharlouei, Alireza Fallah Nosratabad, Hossein Kari Dolat Abad","doi":"10.1080/01490451.2023.2196288","DOIUrl":"https://doi.org/10.1080/01490451.2023.2196288","url":null,"abstract":"Abstract Using the inherent capacity of the soil to meet the nutritional needs of plants is considered an efficient and environmentally friendly solution. In order to study the survival of different phosphate-solubilizing fungi in different combinations of rock phosphate and organic matter as the basis of biofertilizer with the aim of producing an effective formula of biological phosphorus granular fertilizer, this study was conducted as a factorial experiment based on a completely randomized design with three replications. For this purpose, after isolating phosphate-solubilizing fungal strains from agricultural soils in different regions of Iran (Fars, Kermanshah and Razavi Khorasan provinces), the superior strains with high growth stimulating capability were selected for bio-fertilizer formulation based on determination of fungi survival. In order to study the survival of the superior strains [including Talaromyces pinophilus strain 1FB, T. pinophilus strain MFA, Talaromyces verruculosus strain PF157-2 and Aspergillus tubingensis strain PF140-2], six levels of carrier including 100% rock phosphate, 80% rock phosphate + 20% organic matter, 60% rock phosphate + organic matter 40%, 40% rock phosphate + 60% organic matter, 20% rock phosphate + 80% organic matter and 10% rock phosphate + 90% organic matter were used in three temperature levels (28, 40 and 50 °C) during eight time periods (15 days, 30 days, 45 days, 60 days, 75 days, 90 days, 105 days and 120 days) under incubation condition. The results showed that based on the longer time of survival, and the instruction of Soil and Water Research Institute, Karaj, Iran, for the minimum acceptable number of phosphate solubilizing fungi in granulated phosphate microbial fertilizer, a granulated phosphate microbial fertilizer included each of the isolates (respectively PF 157-2 > PF 140-2 > MFA≫ 1FB), a carrier with at least 20% rock phosphate + at least 20% organic matter, and at the temperature of 28 or 40 °C may be developed and recommended in the agricultural soils as an environment-friendly source of phosphorus.","PeriodicalId":12647,"journal":{"name":"Geomicrobiology Journal","volume":"40 1","pages":"493 - 500"},"PeriodicalIF":2.3,"publicationDate":"2023-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49627508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Response of Bacterial Communities to Heavy Metal Contamination in an Abandoned Chromate Factory","authors":"Bing Shan, Ruixia Hao, Hui Xu, Jiani Li, Junman Zhang, Yinhuang Li, Yubo Ye, Anhuai Lu","doi":"10.1080/01490451.2023.2196274","DOIUrl":"https://doi.org/10.1080/01490451.2023.2196274","url":null,"abstract":"Abstract The chromium slags left by industry emission has caused serious damage to the local ecological environment, and microorganisms were sensitive to the variation in the surrounding environmental factors. In this work, 14 soil samples around an abandoned chromate factory were collected. We utilized the 16S rRNA regions from soil DNA to explore the possible effects of environmental factors on soil bacterial communities. High-throughput DNA sequencing results suggested that bacterial communities varied greatly from different soil samples, but Actinobacteria and Proteobacteria were predominant in all samples in the phylum level. In addition, Pseudarthrobacter, Thiobacillus, Paenisporosarcina, Sphingomonas, and Bacillus were abundant at a more refined species level. Based on redundancy analysis (RDA) and variation partitioning analysis (VPA), the results revealed that pH value, soil organic carbon (SOC), total nitrogen (TN), total phosphorus (TP), chromium (Cr) and lead (Pb) could obviously affect the bacterial community structure. Heavy metals, soil nutrients, and pH could explain 37.69%, 13.61%, and 31.41% of bacterial community variation, respectively. This study would provide a theoretical basis for future remediation of local Cr-contaminated soil.","PeriodicalId":12647,"journal":{"name":"Geomicrobiology Journal","volume":"40 1","pages":"462 - 472"},"PeriodicalIF":2.3,"publicationDate":"2023-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47999323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physicochemical Properties, Heavy Metal Composition, and Metal Resistant Microorganisms Associated with Gold Mine Tailings in Jebba, Nigeria","authors":"A. El-Imam, F. B. Auta, H. Okoro, M. Orosun, Ahmad M. Olabode, Adeola R. Bamidele","doi":"10.1080/01490451.2023.2196278","DOIUrl":"https://doi.org/10.1080/01490451.2023.2196278","url":null,"abstract":"Abstract Mine tailings are a source of unique microorganisms with novel characteristics. This study aims to determine the chemical composition of the soil and water from different locations of an active artisanal gold mine and the microorganisms associated with the mine tailings. The physiochemical analysis of tailings soil and water, HM species, and their carcinogenic and non-carcinogenic effects were determined. Heavy metal resistant bacteria (HMRB) were then isolated using nutrient agar supplemented with metals. The results showed that pH and temperature of the soil and water samples ranged between 6.2- 6.6 and 29–30 °C respectively. The estimated incremental lifetime cancer risk (ILCR) from one location was 7.9E − 6 was the highest. The HMs detected includes Arsenic (As), Mercury (Hg), Cadmium (Cd), and Lead (Pb). Twenty-one HMRB were isolated and they are putatively identified as Staphylococcus spp., Bacillus sp., Salinicoccus halodurans, Staphylococcus chromogenes, Paenibacillus cellulositrophicus, Paenibacillus residui, Sporosarcina globispora, and Streptococcus sp. Analysis of the 16S RNA genes sequences revealed that isolate MS2 has 98.01% similarity to Aneurinibacillus aneurinilyticus. The presence and levels of HMs elucidates the high risk of cancerous and non-cancerous effects borne by artisan gold miners. The isolated HMRB can be further analyzed for possible bioremediation of the site.","PeriodicalId":12647,"journal":{"name":"Geomicrobiology Journal","volume":"40 1","pages":"473 - 484"},"PeriodicalIF":2.3,"publicationDate":"2023-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42903511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biofabrication of Selenium Nanoparticles Using Marine Streptomyces sp. and Assessment of Its Antibacterial, Antibiofilm, Antioxidant, and In Vivo Cytotoxic Potential","authors":"D. Sumithra, S. Bharathi, P. Kaviyarasan, G. Suresh","doi":"10.1080/01490451.2023.2196280","DOIUrl":"https://doi.org/10.1080/01490451.2023.2196280","url":null,"abstract":"Abstract In recent days, there is increasing demand for the benign production of selenium nanoparticles (SeNPs) over synthetic methods. Hence in this present study SeNPs were synthesized using the cultural supernatant of marine actinobacterium Streptomyces sp. MA4. The UV spectral analysis of synthesized SeNPs showed a characteristic peak at 290 nm. The HR-SEM analysis confirmed that the synthesized SeNPs were spherical in shape with the average diameter of 50–70 nm and EDX analysis proved that selenium exists as the major constituent of SeNPs. FTIR analysis evidenced the occurrence of peptides, amides, and aldehydes as capping and reducing agents. The synthesized SeNPs is shown to possess broad spectrum antibacterial activity against tested bacteria with the maximum zone of inhibition of 31.3 ± 0.4 mm against Pseudomonas aeruginosa. The MIC and MBC of SeNPs against P. aeruginosa were found to be 128 µg/100 µl. In the anti-biofilm assay, it was also found that biofilm formation by P. aeruginosa was inhibited by SeNPs at the concentration higher than 25 µg/ml. The antioxidant activity against DPPH free radical increases with increasing concentrations of SeNPs. Further in vivo cytotoxicity of SeNPs was tested by brine shrimp larvicidal assay that showed low toxicity with LC50 168.5 µg/ml.","PeriodicalId":12647,"journal":{"name":"Geomicrobiology Journal","volume":"40 1","pages":"485 - 492"},"PeriodicalIF":2.3,"publicationDate":"2023-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42811640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}