从侵蚀生态系统土壤中分离的Ureolysis Bacillus sp.的微生物诱导煅烧沉淀（MICP）对稳定和提高侵蚀土壤肥力的潜力

IF 2.2 4区环境科学与生态学 Q3 ENVIRONMENTAL SCIENCES

Geomicrobiology Journal Pub Date : 2023-05-19 DOI:10.1080/01490451.2023.2211077

Somaye Fazelikia, S. A. Abtahi, M. Kargar, Mojtaba Jafarinia

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引用次数: 2

摘要

水土流失对生态系统和粮食安全具有负面影响。芽孢杆菌具有较高的分解代谢能力，是应用于生物胶结工艺的合适人选。本研究的目的是从不同的生态系统中分离和鉴定具有生物固结能力的芽孢杆菌。从400份样品中分离得到分离菌株，通过生化和分子方法对gyrA和16S rRNA基因进行扩增和测序分析。采用扫描电镜(SEM)、x射线衍射(XRD)和风洞分析等方法测定了不同盐度、pH和温度下尿素存在下的生长情况。从环境样品中共检出195株，其中尿素酶阳性芽孢杆菌25株(12.82%)，分属10种，其中枯草芽孢杆菌5株(20%)，枯草芽孢杆菌和西海芽孢杆菌4株(16%)，流动芽孢杆菌、假firmus芽孢杆菌、cohnii芽孢杆菌、蜡样芽孢杆菌、碱化芽孢杆菌各2株(8%)，球形芽孢杆菌和巨芽孢杆菌各1株(4%)。检出15株脲酶阳性菌株(7.7%)，分别属于拉氏菌属、放线菌属和盐单胞菌属。微生物诱导方解石沉淀(MICP)的最佳条件是30°C, pH 9和6%的盐度。碳酸钙生成率和脲酶活性最高的是枯草芽孢杆菌，碳酸钙用量为24.15 mg/mL，脲酶用量为4.40 × 103单位/L，其次是移动芽孢杆菌和碱芽孢杆菌，碳酸钙用量为22.85 mg/mL，脲酶用量为3.93 × 103单位/L。在流量为90 km/h时，MICP后土壤流失率最低的是枯草芽孢杆菌，减少了100倍，其次是seohaeanensis，其次是蜡样芽孢杆菌，分别减少了90倍、85倍和80倍。结果表明，芽孢杆菌的多样性提供了适应恶劣和未开发环境的潜在能力，也表明在土壤表面利用MICP可以在减少土壤风蚀损失方面发挥非常重要的作用。

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Microbial Induced Calcite Precipitation (MICP) Potential of Ureolytic Bacillus sp. Isolated from the Soil of Eroded Ecosystems for Stabilizing and Improving the Fertility of Eroded Soils

Abstract The loss of soil from lands due to erosion has a negative effect on ecosystems and food security. Bacillus due to high catabolic capability is an appropriate candidate for application in biocementation process. The aim of this study is isolate and characterize Bacillus sp. with biocementation capability from various ecosystems. The isolates were separated from 400 samples, and characterized by biochemical and molecular methods include the amplification and sequencing analysis of gyrA and 16S rRNA genes. Growth in presence of urea, in different salinity, pH, and temperature, also scanning electron microscope (SEM), X-ray diffraction (XRD), and wind tunnel analysis were applied to determine biocementation ability. A total number of 195 isolates were recovered from environmental samples, of which 25 isolates (12.82%) were identified as urease-positive Bacillus which belonged to 10 species consisting of Bacillus subtilis five strains (20%), Bacillus vallismortis and Bacillus seohaeanensis four strains (16%) each, Bacillus mobilis, Bacillus pseudofirmus, Bacillus cohnii, Bacillus cereus, Bacillus alkalinitrilicus two strains (8%) each, and Bacillus sphaericus and Bacillus megaterium one strain (4%) each. Moreover, 15 urease-positive isolates (7.7%) belonging to Ralostenia, Actinomycete, and Halomonas genera were identified. Optimum conditions for microbial induced calcite precipitation (MICP) by isolates are 30 °C, pH 9, and 6% salinity. The highest rate of calcium carbonate formation and urease activity recorded in B. subtilis with 24.15 mg/mL of calcium carbonate and 4.40 × 103 unit/L of urease, followed by B. mobilis and B. alkalinitrilicus with 22.85 mg/mL of calcium carbonate and 3.93 × 103 unit/L of urease. After MICP the lowest soil loss ratio at a flow rate of 90 km/h, was observed in B. subtilis 100-fold reduction, followed by B. seohaeanensis, B. cereus, B. vallismortis, with 90, 85, 80-folds reduction, respectively. Results indicate that the diversity of Bacillus sp. offers the potential ability for adaptation to harsh and untapped environments, also showed that the use of MICP on the soil surface can have a very significant role in reducing soil losses due to wind erosion.

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来源期刊

Geomicrobiology Journal 环境科学-地球科学综合

CiteScore

4.80

自引率

8.70%

发文量

审稿时长

3.3 months

期刊介绍： Geomicrobiology Journal is a unified vehicle for research and review articles in geomicrobiology and microbial biogeochemistry. One or two special issues devoted to specific geomicrobiological topics are published each year. General articles deal with microbial transformations of geologically important minerals and elements, including those that occur in marine and freshwater environments, soils, mineral deposits and rock formations, and the environmental biogeochemical impact of these transformations. In this context, the functions of Bacteria and Archaea, yeasts, filamentous fungi, micro-algae, protists, and their viruses as geochemical agents are examined. Articles may stress the nature of specific geologically important microorganisms and their activities, or the environmental and geological consequences of geomicrobiological activity. The Journal covers an array of topics such as: microbial weathering; microbial roles in the formation and degradation of specific minerals; mineralization of organic matter; petroleum microbiology; subsurface microbiology; biofilm form and function, and other interfacial phenomena of geological importance; biogeochemical cycling of elements; isotopic fractionation; paleomicrobiology. Applied topics such as bioleaching microbiology, geomicrobiological prospecting, and groundwater pollution microbiology are addressed. New methods and techniques applied in geomicrobiological studies are also considered.