Isolation of Inorganic Phosphorus-Solubilizing Bacteria from the Rhizosphere of Festuca arundinacea Schreb.

IF 2.2 4区环境科学与生态学 Q3 ENVIRONMENTAL SCIENCES

Geomicrobiology Journal Pub Date : 2023-05-19 DOI:10.1080/01490451.2023.2208096

Yinping Liang, Zhiyu Xu, Qingfang Xu, Xiang Zhao, Shuaishuai Niu, Xiongyu Yin

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引用次数: 0

Abstract

Abstract Inorganic phosphorus-solubilizing bacteria promote plant growth. However, different strains have different effects. The objective of this study was to screen efficient inorganic phosphorus-solubilizing bacteria (iPSB) from the rhizosphere of Festuca arundinacea Schreb. using Pikovskaya (PVK) medium. The results showed that 43 iPSB isolates were screened by clear halo zone production on PVK medium. Among them, the phosphate solubilization index (PSI) on the PVK medium of 31 iPSBs was calculated to be more than 1.5 at 28 °C in 7 days and ranged from 1.52 to 2.63. The best 31 iPSB isolates were selected for further study, including molecular identification using the 16S rRNA gene, testing of their P solubilization capacity in PVK broth medium, and the detection of catalase activity and organic acid and siderophore production. Among the 31 isolates, the phosphate solubilization of 8 isolates ranged from 203.96 to 412.22 µg/mL, and the value was significantly larger than that of the PVK broth medium without any bacterial isolates. These isolates were identified and belonged to Acinetobacter calcoaceticus, Buttiauxella sp., and Erwinia piriflorinigrans by sequence analysis of the 16S rRNA gene. These iPSB bacterial isolates enrich plant growth-promoting rhizobacteria and can promote the development of sustainable agriculture as phosphate fertilizer synergists.

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圆羊茅根际无机增磷菌的分离。

无机溶磷菌促进植物生长。然而，不同的菌株有不同的效果。本研究旨在从羊茅根际筛选高效无机溶磷菌(iPSB)。使用Pikovskaya (PVK)培养基。结果表明，在PVK培养基上筛选到43株iPSB分离株。其中，31株iPSBs在PVK培养基上28℃条件下7天的磷酸盐增溶指数(PSI)均大于1.5，范围为1.52 ~ 2.63。选取31株最佳iPSB分离株进行16S rRNA基因分子鉴定、PVK肉汤培养基中P溶解能力检测、过氧化氢酶活性检测、有机酸和铁载体产量检测等研究。在31株分离菌中，8株分离菌的磷酸盐增溶量在203.96 ~ 412.22µg/mL之间，显著大于未分离细菌的PVK肉汤培养基。经16S rRNA基因序列分析，鉴定为钙酸不动杆菌、Buttiauxella sp.和piriflorinigans。这些iPSB分离菌丰富了促进植物生长的根瘤菌，可以作为磷肥增效剂促进可持续农业的发展。

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来源期刊

Geomicrobiology Journal 环境科学-地球科学综合

CiteScore

4.80

自引率

8.70%

发文量

审稿时长

3.3 months

期刊介绍： Geomicrobiology Journal is a unified vehicle for research and review articles in geomicrobiology and microbial biogeochemistry. One or two special issues devoted to specific geomicrobiological topics are published each year. General articles deal with microbial transformations of geologically important minerals and elements, including those that occur in marine and freshwater environments, soils, mineral deposits and rock formations, and the environmental biogeochemical impact of these transformations. In this context, the functions of Bacteria and Archaea, yeasts, filamentous fungi, micro-algae, protists, and their viruses as geochemical agents are examined. Articles may stress the nature of specific geologically important microorganisms and their activities, or the environmental and geological consequences of geomicrobiological activity. The Journal covers an array of topics such as: microbial weathering; microbial roles in the formation and degradation of specific minerals; mineralization of organic matter; petroleum microbiology; subsurface microbiology; biofilm form and function, and other interfacial phenomena of geological importance; biogeochemical cycling of elements; isotopic fractionation; paleomicrobiology. Applied topics such as bioleaching microbiology, geomicrobiological prospecting, and groundwater pollution microbiology are addressed. New methods and techniques applied in geomicrobiological studies are also considered.