Frontiers in Neuroscience最新文献

{"title":"Real-time behavioral monitoring of C57BL/6J mice during reproductive cycle.","authors":"Ariane Khatiz, Cassidy Tomlinson, Bohdana Ruzhytska, Erika Kathe Croft, Abdelaziz Amrani, Shannon Dunn, Adrianna Mendrek, Denis Gris","doi":"10.3389/fnins.2025.1509822","DOIUrl":"https://doi.org/10.3389/fnins.2025.1509822","url":null,"abstract":"<p><strong>Introduction: </strong>The present study aims to identify differences in behavioral profiles in post-pubertal C57BL/6J males and female mice across distinct phases of the reproductive cycle in a home cage environment.</p><p><strong>Methods: </strong>To reduce human bias, we used an automated behavioral analysis system <i>HomeCageScan</i> from CleverSys Inc. Mice were monitored continuously, and resulting data were summarized across 24-h, light, and dark cycles. Behavioral activities of each period were analyzed using hierarchical clustering, factor analysis, and principal component analysis.</p><p><strong>Results: </strong>Females exhibited higher levels of physically demanding activities, including ambulatory and exploratory movements, particularly during estrus and metestrus, with estrus showing up to 30% more activity than males. In contrast, males consistently engaged in more sleep-related behaviors across all phases, with significantly higher engagement during the light cycle compared to females in proestrus and estrus (<i>p</i> < 0.0001); the extent of this sex difference was greater during proestrus and estrus than in metestrus and diestrus (<i>p</i> < 0.01). Notably, distinct patterns of sleep fragmentation were observed, with females experiencing greater disruptions during the light cycle, while males showed similar disruptions during the dark cycle. Feeding and resourcing behaviors were highest in males, showing up to 20% increase compared to cycling females, as well as significantly engaging in habituation-related behaviors such as feeding and digging. Interphase differences were observed within females, such as a significant increase of habituation-related activities during estrus compared to proestrus and diestrus (<i>p</i> < 0.05), while during the dark cycle, these activities peaked during the diestrus phase (<i>p</i> < 0.05). Female mice in the metestrus phase exhibited more sleep-related behaviors than those in proestrus.</p><p><strong>Discussion: </strong>Our study has revealed prevalent behavioral differences due to sex, and inter-phase variations by employing a continuous monitoring approach designed to reduce bias. This methodology ensures a comprehensive understanding of natural behavioral patterns and strategies.</p>","PeriodicalId":12639,"journal":{"name":"Frontiers in Neuroscience","volume":"19 ","pages":"1509822"},"PeriodicalIF":3.2,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11911481/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143648259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mutation in <i>Wdr45</i> leads to early motor dysfunction and widespread aberrant axon terminals in a beta-propeller protein associated neurodegeneration (BPAN) patient-inspired mouse model.","authors":"Brandon L Meyerink, Krishna S Karia, Mitchell J Rechtzigel, Prithvi R Patthi, Ariana C Edwards, Jessica M Howard, Elizabeth R Aaseng, Shamiq Aftab, Jill M Weimer, Louis-Jan Pilaz","doi":"10.3389/fnins.2025.1545004","DOIUrl":"https://doi.org/10.3389/fnins.2025.1545004","url":null,"abstract":"<p><p>Beta-propeller Protein Associated Neurodegeneration (BPAN) is a devastating neurodevelopmental and neurodegenerative disease linked to variants in <i>WDR45</i>. Currently, there is no cure or disease altering treatment for this disease. This is, in part, due to a lack of insight into early phenotypes of BPAN progression and <i>WDR45</i>'s role in establishing and maintaining neurological function. Here we generated and characterized a mouse model bearing a c52C > T BPAN patient variant in <i>Wdr45.</i> We show this mutation ablates <i>WDR45</i> protein expression and alters autophagy in the brain. Behavioral analysis of these mice revealed characteristic signs of BPAN including cognitive impairment, hyperactivity, and motor decline. We show these behaviors coincide with widespread glial activation and early development of axonal spheroids in multiple neuron subclasses throughout the brain. Several lines of evidence suggest these spheroids arise from axon terminals. Transcriptomic analysis uncovered multiple disrupted pathways in the cortex including genes associated with synapses, neurites, endosomes, endoplasmic reticulum, and ferroptosis. This is supported by accumulation of the iron regulating transferrin receptor 1 (TFRC) and the endoplasmic reticulum resident calreticulin (CALR) in the cortex as these animals age. CALR forms spheroid structures similar to the axonal spheroids seen in these animals. Taken together, our data suggest that WDR45 is necessary for healthy brain function and maintenance of axon terminals. This model opens the door to therapeutics targeting BPAN and further exploration of the role of WDR45 in neuronal function.</p>","PeriodicalId":12639,"journal":{"name":"Frontiers in Neuroscience","volume":"19 ","pages":"1545004"},"PeriodicalIF":3.2,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11907653/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143648028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The assessment of autonomic nervous function in patients with gastrointestinal malignancies and its relationship with clinical characteristics.","authors":"Zhang Xiwen, Feng Qiyun, Li Chuqiao, Jiang Anqi, Wu Zhenzhen, Deng Qiong, Peng Yu, Wang Chunlin","doi":"10.3389/fnins.2025.1542224","DOIUrl":"https://doi.org/10.3389/fnins.2025.1542224","url":null,"abstract":"<p><strong>Introduction: </strong>The gastrointestinal tract is the organ most extensively distributed by autonomic nerves, and researches have indicated a relationship between automatic nerves and the progression of gastrointestinal cancers. This study aimed to evaluate the autonomic nervous function in patients with gastrointestinal cancer and to explore its relationship with clinical characteristics.</p><p><strong>Methods: </strong>We employed the Composite Autonomic Symptom Score 31 (COMPASS-31) questionnaire and cardiovascular autonomic reflex tests (CARTs) to evaluate autonomic nervous function, while also conducting a thorough analysis of clinical data.</p><p><strong>Results: </strong>Our results showed that low white blood cell (WBC) count (OR = 0.461, 95% CI: 0.218-0.976, <i>p</i> = 0.043) and increased maximum tumor diameter (OR = 1.619, 95% CI: 1.025-2.555, <i>p</i> = 0.039) were risk factors for autonomic dysfunction according to the COMPASS-31 assessment. While hypertension (OR = 5.747, 95% CI: 1.186-27.862, <i>p</i> = 0.030) and elevated platelet-to-albumin ratio (PAR) (OR = 1.256, 95% CI: 1.025-1.540, <i>p</i> = 0.028) were identified as independent risk factors for autonomic dysfunction based on the CARTs results. Combining the findings from COMPASS-31 and CARTs revealed that older age (OR = 1.133, 95% CI: 1.015-1.264, <i>p</i> = 0.027) and vascular invasion (OR = 7.706, 95% CI: 1.391-42.684, <i>p</i> = 0.019) were also independent risk factors for autonomic dysfunction.</p><p><strong>Conclusion: </strong>Our findings reveal that these specific factors related to gastrointestinal cancers significantly influence autonomic nervous function. It is essential to evaluate autonomic nervous function and its associated risk factors in patients with gastrointestinal malignancies, which provide new insights into the intervention strategies for cancer diseases.</p>","PeriodicalId":12639,"journal":{"name":"Frontiers in Neuroscience","volume":"19 ","pages":"1542224"},"PeriodicalIF":3.2,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11906409/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143648239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Music in the loop: a systematic review of current neurofeedback methodologies using music.","authors":"Alexandre Sayal, Bruno Direito, Teresa Sousa, Neomi Singer, Miguel Castelo-Branco","doi":"10.3389/fnins.2025.1515377","DOIUrl":"https://doi.org/10.3389/fnins.2025.1515377","url":null,"abstract":"<p><p>Music, a universal element in human societies, possesses a profound ability to evoke emotions and influence mood. This systematic review explores the utilization of music to allow self-control of brain activity and its implications in clinical neuroscience. Focusing on music-based neurofeedback studies, it explores methodological aspects and findings to propose future directions. Three key questions are addressed: the rationale behind using music as a stimulus, its integration into the feedback loop, and the outcomes of such interventions. While studies emphasize the emotional link between music and brain activity, mechanistic explanations are lacking. Additionally, there is no consensus on the imaging or behavioral measures of neurofeedback success. The review suggests considering whole-brain neural correlates of music stimuli and their interaction with target brain networks and reward mechanisms when designing music-neurofeedback studies. Ultimately, this review aims to serve as a valuable resource for researchers, facilitating a deeper understanding of music's role in neurofeedback and guiding future investigations.</p>","PeriodicalId":12639,"journal":{"name":"Frontiers in Neuroscience","volume":"19 ","pages":"1515377"},"PeriodicalIF":3.2,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11906423/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143648013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Decreased scene-selective activity within the posterior intraparietal cortex in amblyopic adults.","authors":"Sarala N Malladi, Jan Skerswetat, Marianna E Schmidt, Roger B H Tootell, Eric D Gaier, Peter J Bex, David G Hunter, Shahin Nasr","doi":"10.3389/fnins.2025.1527148","DOIUrl":"https://doi.org/10.3389/fnins.2025.1527148","url":null,"abstract":"<p><strong>Introduction: </strong>Amblyopia is a developmental disorder associated with reduced performance in visually guided tasks, including binocular navigation within natural environments. To help understand the underlying neurological disorder, we used fMRI to test the impact of amblyopia on the functional organization of scene-selective cortical areas, including the posterior intraparietal gyrus scene-selective (PIGS) area, a recently discovered region that responds selectively to ego-motion within naturalistic environments.</p><p><strong>Methods: </strong>Nineteen amblyopic adults (10 females) and thirty age-matched controls (15 females) participated in this study. Amblyopic participants spanned a wide range of amblyopia severity, based on their interocular visual acuity difference and stereoacuity. The visual function questionnaire (VFQ-39) was used to assess the participants' perception of their visual capabilities.</p><p><strong>Results: </strong>Compared to controls, we found weaker scene-selective activity within the PIGS area in amblyopic individuals. By contrast, the level of scene-selective activity across the occipital place area (OPA), parahippocampal place area (PPA), and retrosplenial cortex (RSC) remained comparable between amblyopic and control participants. The participants' scores on \"general vision\" (VFQ-39 subscale) correlated with the level of scene-selective activity in PIGS.</p><p><strong>Discussion: </strong>These results provide novel and direct evidence for the impact of amblyopia on scene processing within the human brain, thus enabling future studies to potentially link these changes across the spectrum of documented disabilities in amblyopia.</p>","PeriodicalId":12639,"journal":{"name":"Frontiers in Neuroscience","volume":"19 ","pages":"1527148"},"PeriodicalIF":3.2,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11907652/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143648011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Behavioral alterations in antibiotic-treated mice associated with gut microbiota dysbiosis: insights from 16S rRNA and metabolomics.","authors":"Asma Bibi, Famin Zhang, Jilong Shen, Ahmad Ud Din, Yuanhong Xu","doi":"10.3389/fnins.2025.1478304","DOIUrl":"https://doi.org/10.3389/fnins.2025.1478304","url":null,"abstract":"<p><p>The gut and brain interact through various metabolic and signaling pathways, each of which influences mental health. Gut dysbiosis caused by antibiotics is a well-known phenomenon that has serious implications for gut microbiota-brain interactions. Although antibiotics disrupt the gut microbiota's fundamental structure, the mechanisms that modulate the response and their impact on brain function are still unclear. It is imperative to comprehend and investigate crucial regulators and factors that play important roles. We aimed to study the effect of long-term antibiotic-induced disruption of gut microbiota, host metabolomes, and brain function and, particularly, to determine the basic interactions between them by treating the C57BL/6 mice with two different, most commonly used antibiotics, ciprofloxacin and amoxicillin. Anxiety-like behavior was confirmed by the elevated plus-maze test and open field test. Gut microbes and their metabolite profiles in fecal, serum, and brain samples were determined by 16S rRNA sequencing and untargeted metabolomics. In our study, long-term antibiotic treatment exerted anxiety-like effects. The fecal microbiota and metabolite status revealed that the top five genera found were <i>Lactobacillus, Bacteroides, Akkermansia, Ruminococcus_gnavus_</i>group, and unclassified <i>norank_f_Muribaculaceae</i>. The concentration of serotonin, L-Tyrosine, 5-Hydroxy-L-tryptophan, L-Glutamic acid, L-Glutamate, 5-Hydroxyindole acetic acid, and dopaminergic synapsis was comparatively low, while adenosine was high in antibiotic-treated mice. The KEGG enrichment analysis of serum and brain samples showed that amino acid metabolism pathways, such as tryptophan metabolism, threonine metabolism, serotonergic synapsis, methionine metabolism, and neuroactive ligand-receptor interaction, were significantly decreased in antibiotic-treated mice. Our study demonstrates that long-term antibiotic use induces gut dysbiosis and alters metabolic responses, leading to the dysregulation of brain signaling molecules and anxiety-like behavior. These findings highlight the complex interactions between gut microbiota and metabolic functions, providing new insights into the influence of microbial communities on gut-brain communication.</p>","PeriodicalId":12639,"journal":{"name":"Frontiers in Neuroscience","volume":"19 ","pages":"1478304"},"PeriodicalIF":3.2,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11906700/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143647933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serum-derived exosomal microRNAs as biomarkers for postoperative delirium.","authors":"Maokai Xu, Yingjie Chen, Yujun Lin, Danfeng Wang, Xiaochun Zheng","doi":"10.3389/fnins.2025.1525230","DOIUrl":"https://doi.org/10.3389/fnins.2025.1525230","url":null,"abstract":"<p><strong>Introduction: </strong>Postoperative delirium (POD) is a frequent and challenging complication in elderly surgical patients, marked by abrupt cognitive and attentional disturbances. Current POD diagnosis depends on clinical assessments that are time-intensive and lack predictive accuracy before surgery. Although previous research has explored biomarkers such as neuroinflammatory factors and Alzheimer's-related proteins to enhance POD prediction, single molecular markers have proven insufficient for reliable prognosis.</p><p><strong>Methods: </strong>This study investigated serum exosomal miRNA expression profiles in postoperative patients to assess their association with POD. We compared miRNA expression between POD and non-POD groups through cognitive assessments and serum analyses. Additionally, enrichment analysis was conducted to determine the biological pathways regulated by differentially expressed miRNAs.</p><p><strong>Results: </strong>Our analysis identified 57 miRNAs with significantly altered expression between POD and non-POD patients, including 16 upregulated and 41 downregulated miRNAs in the POD group. Enrichment analysis revealed that these miRNAs are involved in genes regulating neurotrophin signaling, neuroactive ligand-receptor interactions, and pathways that influence neuronal plasticity and cell viability.</p><p><strong>Discussion: </strong>This study highlights specific miRNAs as potential biomarkers for POD and suggests their involvement in the underlying mechanisms of cognitive decline following surgery. By enhancing diagnostic capabilities and identifying potential therapeutic targets, our findings could lead to more effective POD management strategies for elderly patients. Further research is recommended to validate these miRNAs and evaluate their clinical utility for predictive screening and therapeutic interventions.</p>","PeriodicalId":12639,"journal":{"name":"Frontiers in Neuroscience","volume":"19 ","pages":"1525230"},"PeriodicalIF":3.2,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11906430/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143648236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physical activity and neuroplasticity in neurodegenerative disorders: a comprehensive review of exercise interventions, cognitive training, and AI applications.","authors":"Lamia Ben Ezzdine, Wissem Dhahbi, Ismail Dergaa, Halil İbrahim Ceylan, Noomen Guelmami, Helmi Ben Saad, Karim Chamari, Valentina Stefanica, Abdelfatteh El Omri","doi":"10.3389/fnins.2025.1502417","DOIUrl":"https://doi.org/10.3389/fnins.2025.1502417","url":null,"abstract":"<p><p>This review aimed to elucidate the mechanisms through which (i) physical activity (PA) enhances neuroplasticity and cognitive function in neurodegenerative disorders, and (ii) identify specific PA interventions for improving cognitive rehabilitation programs. We conducted a literature search in PubMed, Medline, Scopus, Web of Science, and PsycINFO, covering publications from January 1990 to August 2024. The search strategy employed key terms related to neuroplasticity, physical exercise, cognitive function, neurodegenerative disorders, and personalized physical activity. Inclusion criteria included original research on the relationship between PA and neuroplasticity in neurodegenerative disorders, while exclusion criteria eliminated studies focusing solely on pharmacological interventions. The review identified multiple pathways through which PA may enhance neuroplasticity, including releasing neurotrophic factors, modulation of neuroinflammation, reduction of oxidative stress, and enhancement of synaptic connectivity and neurogenesis. Aerobic exercise was found to increase hippocampal volume by 1-2% and improve executive function scores by 5-10% in older adults. Resistance training enhanced cognitive control and memory performance by 12-18% in elderly individuals. Mind-body exercises, such as yoga and tai-chi, improved gray matter density in memory-related brain regions by 3-5% and enhanced emotional regulation scores by 15-20%. Dual-task training improved attention and processing speed by 8-14% in individuals with neurodegenerative disorders. We also discuss the potential role of AI-based exercise and AI cognitive training in preventing and rehabilitating neurodegenerative illnesses, highlighting innovative approaches to personalized interventions and improved patient outcomes. PA significantly enhances neuroplasticity and cognitive function in neurodegenerative disorders through various mechanisms. Aerobic exercise, resistance training, mind-body practices, and dual-task exercises each offer unique cognitive benefits. Implementing these activities in clinical settings can improve patient outcomes. Future research should focus on creating personalized interventions tailored to specific conditions, incorporating personalized physical exercise programs to optimize cognitive rehabilitation.</p>","PeriodicalId":12639,"journal":{"name":"Frontiers in Neuroscience","volume":"19 ","pages":"1502417"},"PeriodicalIF":3.2,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11906675/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143648256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Trajectories of working memory and decision making abilities along juvenile development in mice.","authors":"Ann Marlene Thies, Irina Pochinok, Annette Marquardt, Maria Dorofeikova, Ileana L Hanganu-Opatz, Jastyn A Pöpplau","doi":"10.3389/fnins.2025.1524931","DOIUrl":"https://doi.org/10.3389/fnins.2025.1524931","url":null,"abstract":"<p><p>Rodents commonly serve as model organisms for the investigation of human mental disorders by capitalizing on behavioral commonalities. However, our understanding of the developmental dynamics of complex cognitive abilities in rodents remains incomplete. In this study, we examined spatial working memory as well as odor-and texture-based decision making in mice using a delayed non-match to sample task and a two-choice set-shifting task, respectively. Mice were investigated during different stages of development: pre-juvenile, juvenile, and young adult age. We show that, while working memory abilities in mice improve with age, decision making performance peaks during juvenile age by showing a sex-independent trajectory. Moreover, cFos expression, as a first proxy for neuronal activity, shows distinct age-and brain area-specific changes that relate to task-specific behavioral performance. The distinct developmental trajectories of working memory and decision making in rodents resemble those previously reported for humans.</p>","PeriodicalId":12639,"journal":{"name":"Frontiers in Neuroscience","volume":"19 ","pages":"1524931"},"PeriodicalIF":3.2,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11906447/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143648242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Photochemically induced thrombosis combined with chronic restraint stress for modeling post-stroke depression in mice.","authors":"Tumarisi Tuersunjiang, Qingchen Wang, Zhengzheng Wang, Feng Gao, Zhengchun Wang","doi":"10.3389/fnins.2025.1547551","DOIUrl":"https://doi.org/10.3389/fnins.2025.1547551","url":null,"abstract":"<p><strong>Introduction: </strong>Post-stroke depression (PSD) is a prevalent neuropsychiatric disorder associated with impaired recovery in stroke survivors, potentially linked to dysregulation of brain-derived neurotrophic factor (BDNF). This study aimed to establish a novel animal model of PSD by integrating ischemic brain injury with chronic psychological stress.</p><p><strong>Methods: </strong>Mice were subjected to photochemically induced thrombosis (PIT) to generate focal ischemic lesions in the parietal lobe, followed by chronic restraint stress (CRS) to simulate post-stroke psychological stress. Behavioral assessments (sucrose preference test, forced swim test, tail suspension test) and molecular analyses (BDNF, synaptophysin [SYP], interleukin-1 [IL-1], tumor necrosis factor-<i>α</i> [TNF-<i>α</i>]) were conducted to evaluate depressive-like phenotypes and neuroinflammatory markers.</p><p><strong>Results: </strong>The PIT model produced consistent ischemic damage, with an average infarct area of 2.580 ± 0.426% in the parietal lobe. Mice exposed to PIT-CRS exhibited significant depressive-like behaviors, including reduced sucrose preference (<i>p</i> < 0.001), increased immobility time in the forced swim test (<i>p</i> = 0.056), and prolonged immobility in the tail suspension test (<i>p</i> = 0.168) compared to the Sham group. Molecular analyses revealed marked downregulation of BDNF (<i>p</i> = 0.004) and SYP (<i>p</i> = 0.074), alongside upregulated IL-1 (<i>p</i> = 0.024) and TNF-α (<i>p</i> = 0.368) levels in the PIT-CRS group.</p><p><strong>Conclusion: </strong>The PIT-CRS model provides a comprehensive and reproducible platform for studying PSD. By integrating both ischemic injury and chronic stress, this model captures the multifaceted nature of PSD and offers valuable insights into its pathophysiology. Future research using this model could pave the way for the development of targeted therapies for PSD.</p>","PeriodicalId":12639,"journal":{"name":"Frontiers in Neuroscience","volume":"19 ","pages":"1547551"},"PeriodicalIF":3.2,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11906474/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143648201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}