Gastroenterology and Hepatology From Bed to Bench最新文献

{"title":"Microbiome-microRNA interactions in inflammatory bowel disease: insights from metagenomic and transcriptomic data analysis.","authors":"Nesa Kazemifard, Mohammad Hossein Norouzi-Beirami, Shaghayegh Baradaran Ghavami, Leila Ghanbari-Maman, Mohammad Reza Zali, Shabnam Shahrokh, Kaveh Kavousi","doi":"10.22037/ghfbb.v18i4.3186","DOIUrl":"10.22037/ghfbb.v18i4.3186","url":null,"abstract":"<p><strong>Background: </strong>Inflammatory Bowel Disease (IBD) is a chronic inflammation of the gastrointestinal tract, the precise origins of which remain not fully elucidated. This study investigates the complex relationship between gut metagenomics and host transcriptomics in IBD patients, focusing on Ulcerative Colitis (UC) and Crohn's Disease (CD).</p><p><strong>Method: </strong>One proposed theory suggests that microRNAs produced by the host may significantly influence IBD development by impacting the gut microbiota. Conversely, the gut microbiome may regulate the expression of host microRNAs, leading to dysfunction in the intestinal epithelium. An enrichment analysis was conducted to pinpoint associated pathways. To unravel this intricate interplay, the study utilized data from the IBDMDB database, selecting samples from adult individuals.</p><p><strong>Result: </strong>The dataset comprised 50 paired metagenomic and host transcriptomic samples, including 8 controls, 18 UCs, and 24 CDs. Computational analyses and network constructions were applied to identify relationships between bacterial species, microRNAs, and other transcripts.</p><p><strong>Conclusion: </strong>This research offers valuable insights into the dynamic relationship between the gut microbiome and human transcriptomics in IBD, providing a deeper understanding of potential disease mechanisms. Furthermore, it sheds light on the complex tripartite network connecting bacterial species, microRNAs, and transcripts, contributing to a comprehension of IBD pathogenesis and the identification of novel therapeutic targets.</p>","PeriodicalId":12636,"journal":{"name":"Gastroenterology and Hepatology From Bed to Bench","volume":"18 SI","pages":"85-96"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13084485/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147941168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Colon cancer versus colitis: a microRNA and gene expression analysis via system biology tools.","authors":"Fatemeh TajikRostami, Alireza Omidi Farzin, Babak Arjmand, Zahra Razzaghi, Majid Rezaei-Tavirani, Mostafa Rezaei-Tavirani, Behzad Hatami","doi":"10.22037/ghfbb.v18iSpecialIssue.3262","DOIUrl":"10.22037/ghfbb.v18iSpecialIssue.3262","url":null,"abstract":"<p><strong>Aim: </strong>The molecular mechanism of this relationship can be detected that promote the transition from colitis to colon cancer via system biology tools is the main aim of this study.</p><p><strong>Background: </strong>There is a correlation between colitis and associated colon analysis.</p><p><strong>Methods: </strong>The differentially expressed microRNA and gene profiles of samples with colitis and colon cancer are extracted from the Gene Expression Omnibus (GEO) database and assessed using the GEO2R program and the CluePedia plugin of Cytoscape software to identify crucial actors and controlled genes or microRNAs.</p><p><strong>Results: </strong>Analyses revealed microRNA analysis was not suitable tool to detect molecular mechanism of colitis to associated colon cancer in mouse model but the gene expression profile assessment leads to introduce CLASP1, CENPM, KIF18A, PRS7, CENPI, ZWINT, SPC24, and CENPK as the critical genes that promote this transition in fibroblast cells of colon cancer.</p><p><strong>Conclusion: </strong>Regulation of ribosome and kinetochore was suggested as a possible treatment to prevent colitis - colon cancer transition.</p>","PeriodicalId":12636,"journal":{"name":"Gastroenterology and Hepatology From Bed to Bench","volume":"18 SI","pages":"68-75"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13081415/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147940953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"AI-driven prediction of the optimal number of bowel preparation sachets for adequate preparation before colonoscopy.","authors":"Amir Sadeghi, Roxana Peysepar, Neda Kafash, Shabnam Shahrokh, Alireza Ahmadzadeh","doi":"10.22037/ghfbb.v18iSpecialIssue.3196","DOIUrl":"10.22037/ghfbb.v18iSpecialIssue.3196","url":null,"abstract":"<p><strong>Background: </strong>High-quality colonoscopy is critical for colorectal cancer (CRC) prevention. Suboptimal bowel preparation impairs adenoma detection and prolongs cecal intubation time (CIT). This study aimed to identify behavioral and clinical predictors of CIT and bowel preparation quality, to support development of an AI-based model for personalized purgative dosing.</p><p><strong>Method: </strong>In a prospective study of 280 outpatients undergoing screening colonoscopy, variables such as age, sex, BMI, physical activity, sleep duration, and bowel preparation quality (Boston Bowel Preparation Scale (BBPS)) were recorded. CIT, total colonoscopy time (TCT), and polyp/adenoma detection were measured. Regression models and machine learning algorithms (Random Forest, XGBoost) were applied to develop predictive models.</p><p><strong>Result: </strong>More sedentary time 6-9 hours was significantly associated with poor preparation and longer CIT and TCT (p<0.01). Daily activity Time between 9-12 hours correlated with better preparation. Chronic constipation, opioid use, and obesity independently predicted poor bowel prep (BBPS ≤6). Female sex, age ≥60, and BMI ≥30 was also associated with lower BBPS scores. The SVR model achieved a mean absolute error (MAE) of 1.3 and RMSE of 1.6 in predicting optimal powder dosage.</p><p><strong>Conclusion: </strong>Simple behavioral factors such as sleep and physical activity significantly affect colonoscopy performance. Personalized purgative dosing based on individual risk factors may enhance preparation quality and reduce procedural delays. Machine learning models can support clinical decision-making and improve colorectal cancer screening efficacy.</p>","PeriodicalId":12636,"journal":{"name":"Gastroenterology and Hepatology From Bed to Bench","volume":"18 SI","pages":"121-124"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13081414/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147940875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Global and regional trends in under-five diarrheal disease burden: impact of human development index and geographic disparities, 1990-2021.","authors":"Mehdi Azizmohammad Looha, Ali Saberi Shahrbabaki, Azin Mohammadpoor, Mahmoud Zamani, Zahra Sadeghloo, Melika Jameie, Hanieh Mousavi, Seyede Roxane Pooresmaeil Niaki, Hossein Mohebbi, Naghmeh Asadimanesh, Fatemeh Norouzi, Sepideh Banar, Matin Jalalinejad, Maedeh Yousefi, Sofia Shahreki Mojahed, Forough Masheghati, Mehrnoosh Ghalandarlaki, Alireza Bahadorimonfared","doi":"10.22037/ghfbb.v18i2.3158","DOIUrl":"10.22037/ghfbb.v18i2.3158","url":null,"abstract":"<p><strong>Aim: </strong>In this study, global and regional trends between 1990 and 2021 were examined by sex, level of development, and geography, and projected to 2025.</p><p><strong>Background: </strong>Diarrheal disease continues to be a leading cause of morbidity and mortality in children under the age of five (U5).</p><p><strong>Methods: </strong>We assessed GBD 2021 data for U5 children in 204 countries, 21 regions, and 7 super-regions with joinpoint regression to analyze time trends, a hybrid ARIMA-ETS-ANN model to project prevalence and mortality until 2025, and longitudinal multilevel modeling to determine the effect of development level. Spatial clustering in 1990 and 2021 was assessed with Local Moran's I.</p><p><strong>Results: </strong>In 2021, the global prevalence and mortality rates of diarrheal disease among children under five were 885.07 and 51.72 per 100,000 population, respectively. Between 1990 and 2021, global U5 mortality decreased by 80.4%, and prevalence by 71.8%. The heaviest burden remained in Sub-Saharan Africa (SSA) and South Asia (SA), though all super-regions experienced statistically significant decreases. Joinpoint regression across the entire interval (1990-2021) revealed significant overall reductions in mortality (AAPC -5.15; 95% CI: -5.19, -5.10) and prevalence (AAPC -3.98; 95% CI: -4.03, -3.94). Hybrid forecasts predict ongoing decreases through 2025, with prevalence reaching 631.3 and mortality 36.6 per 100,000 population worldwide. Multilevel models revealed steeper annual reductions in low- and medium-Human Development Index (HDI) nations, corroborated by significant time-HDI interaction terms (β=79.76 for prevalence; β=7.50 for mortality; both p<0.001), although such countries had a persisting higher absolute burden. Spatial analysis revealed enduring hotspots in SSA and SA in both 1990 and 2021, and the formation of new clusters in several high-income countries. Coldspots occurred primarily in high-income and island nations.</p><p><strong>Conclusion: </strong>Significant global advancements have lessened the burden of diarrheal disease in U5 children; yet, ongoing disparities attributed to unsafe water, poor sanitation and hygiene, undernutrition, and low maternal education underscore the necessity for combined water, sanitation, and hygiene interventions, rotavirus immunization, and community-based health education in high-risk areas.</p>","PeriodicalId":12636,"journal":{"name":"Gastroenterology and Hepatology From Bed to Bench","volume":"18 2","pages":"177-195"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12421937/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145039938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy and safety of sphingosine-1-phosphate receptor modulators in the management of inflammatory bowel disease: a systematic review and meta-analysis.","authors":"Darmadi Darmadi, Dina Aprillia Ariestine, Herwindo Ahmad, Yan Indra Fajar Sitepu","doi":"10.22037/ghfbb.v18i2.3134","DOIUrl":"10.22037/ghfbb.v18i2.3134","url":null,"abstract":"<p><strong>Aim: </strong>To evaluate the efficacy and safety of sphingosine-1-phosphate (S1P) receptor modulators in treating ulcerative colitis (UC) and Crohn's disease (CD).</p><p><strong>Background: </strong>Inflammatory Bowel Disease (IBD) is a chronic immune-mediated condition that remains challenging to manage. S1P receptor modulators offer a novel therapeutic approach by targeting immune cell trafficking, potentially improving disease outcomes.</p><p><strong>Methods: </strong>A systematic review and meta-analysis were conducted by searching PubMed, Scopus, and Cochrane Library major electronic databases for randomized controlled trials (RCTs) investigating S1P receptor modulators in IBD. Clinical outcomes assessed included clinical remission, clinical response, endoscopic improvement, histologic remission, and serious adverse events. Pooled odds ratios (OR) with 95% confidence intervals (CI) were calculated using a fixed-effects model.</p><p><strong>Results: </strong>Seven RCTs with a total of 2,597 patients were included. S1P receptor modulators significantly improved clinical remission (OR = 1.49, 95% CI: 1.21-1.84, p < 0.001), histologic remission (OR = 1.74, 95% CI: 1.31-2.31, p < 0.001), endoscopic improvement (OR = 1.58, 95% CI: 1.23-2.04, p < 0.001), and clinical response (OR = 1.27, 95% CI: 1.05-1.54, p = 0.01). The risk of serious adverse events did not significantly differ between treatment and placebo groups (OR = 1.28, 95% CI: 0.92-1.80, p = 0.14), suggesting a favorable safety profile.</p><p><strong>Conclusion: </strong>This meta-analysis supports S1P receptor modulators, particularly ozanimod and etrasimod, as effective and safe treatments for UC. Further studies are needed to assess long-term safety and direct comparisons with existing biologic therapies.</p>","PeriodicalId":12636,"journal":{"name":"Gastroenterology and Hepatology From Bed to Bench","volume":"18 2","pages":"120-131"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12421927/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145039984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Could serum uric acid levels be a biomarker for metabolic dysfunction-associated steatotic liver disease?","authors":"Ömer Keskin, Ayşe Özdemir, Yusuf Üstün, Cenk Aypak","doi":"10.22037/ghfbb.v18i3.3033","DOIUrl":"10.22037/ghfbb.v18i3.3033","url":null,"abstract":"<p><strong>Aim: </strong>In this study, we investigated the relationship between serum uric acid (SUA) and metabolic dysfunction-associated steatotic liver disease (MASLD) in patients detected through abdominal ultrasound. The second aim was to determine if there were any gender differences.</p><p><strong>Background: </strong>High SUA levels may contribute to the mechanisms underlying various metabolic disorders, including MASLD.</p><p><strong>Methods: </strong>We conducted a retrospective study among patients older than 18 who had been admitted to a tertiary hospital's gastroenterology outpatient clinic for over two years. A total of 200 patients diagnosed with either Grade 1, 2, or 3 hepatosteatosis by ultrasound screening and 200 patients without hepatosteatosis were included in the study. Patients' demographic features, laboratory parameters, and detailed medical history were extracted from the hospital's medical records.</p><p><strong>Results: </strong>The mean age of the 400 patients enrolled in the study was 47.92±0.78 years, and 70.25% were men. SUA (5.34 mg/dL ± 1.52 vs. 4.64 mg/dL ± 1.28, p=0.001), alanine aminotransferase (ALT) (26.55 U/L ± 23.82 vs. 19.57 U/L ± 20.81, p=0.002), aspartate aminotransferase (AST) (22.68 U/L ± 20.35 vs. 18.07 U/L ± 9.89, p=0.005), and glycosylated hemoglobin (HbA1c) (6.45% ± 1.69 vs. 5.76% ± 1.23, p=0.001) levels were higher in the MASLD group. In the MASLD group, HbA1c, AST, and ALT were significantly higher in the Grade 2-3 group than in the Grade 1 (p=0.038, p=0.038, p=0.001, respectively).</p><p><strong>Conclusion: </strong>Elevated SUA levels are strongly linked to MASLD, particularly in males. Ease of measurement and low cost suggest that SUA could serve as a non-invasive biomarker for MASLD detection in at-risk groups.</p>","PeriodicalId":12636,"journal":{"name":"Gastroenterology and Hepatology From Bed to Bench","volume":"18 3","pages":"342-352"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12535785/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145336795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Algorithmic approach for endoscopic management of colorectal polyps: an up-to-date review.","authors":"Amir Sadeghi, Ali Yousefian Astaneh, Erfan Arabpour, Mohammad Reza Zali","doi":"10.22037/ghfbb.v18i1.3085","DOIUrl":"10.22037/ghfbb.v18i1.3085","url":null,"abstract":"<p><p>With the growing incidence of colorectal cancer worldwide, mainly due to increased early detection in screening programs, usage of endoscopic techniques for management of colorectal lesions have been gaining progressive attention. Each year, more advanced endoscopic techniques come to practice and old ones find technical enhancements. By reviewing guidelines, randomized controlled trials, meta-analyses, and original research, we aim to establish an evidence-based approach for selecting the optimal endoscopic method based on the polyp size, morphology, and classification. We critically analyze the advantages, limitations, and potential complications associated with each technique, providing a comprehensive overview for clinicians and suggest areas which may yet need further studies to be conducted. Our comprehensive review can provide a framework that will help clinicians choose an approach most suitable for their patients. This review attempts to contribute to the optimization of endoscopic management in colorectal polyps, with eventual improvement in patient outcomes.</p>","PeriodicalId":12636,"journal":{"name":"Gastroenterology and Hepatology From Bed to Bench","volume":"18 1","pages":"39-52"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12301536/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144741950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Outcomes after treatment with proton pump inhibitors in patients with ineffective esophageal motility: a systematic review.","authors":"Fatima Sakr, Maryam Soheilipour, Fatemeh Maghool, Elham Tabesh, Serhat Bor, Peyman Adibi","doi":"10.22037/ghfbb.v18i4.3191","DOIUrl":"10.22037/ghfbb.v18i4.3191","url":null,"abstract":"<p><strong>Aim: </strong>This systematic review evaluated the effectiveness of proton pump inhibitors (PPIs) in improving manometric parameters in patients with gastroesophageal reflux disease (GERD) diagnosed with Ineffective esophageal motility (IEM).</p><p><strong>Background: </strong>IEM represents the most common esophageal motility disorder among patients with GERD. To date, no treatment has conclusively demonstrated efficacy in restoring esophageal motor function in these individuals.</p><p><strong>Methods: </strong>This systematic review was performed according to the PRISMA guidelines. We conducted comprehensive searches of electronic databases, including PubMed, Embase, and Scopus, until January 2024 to identify relevant randomized and non-randomized controlled trials. Criteria for study inclusion were based on the population, intervention, comparison, and outcome framework.</p><p><strong>Results: </strong>A total of 9 studies met the inclusion criteria, comprising eight non-randomized controlled trials (non-RCTs) and one randomized controlled trial (RCT). Out of 305 recruited patients with a diagnosis of GERD, 185 patients matched the diagnosis of IEM with manometric studies before and after a PPI treatment. PPIs used in the studies included omeprazole, pantoprazole, lansoprazole, and rabeprazole. The duration of treatment ranged from four to twenty-four weeks. Except for one study, there was no statistically significant improvement in manometric parameters following PPI treatment.</p><p><strong>Conclusion: </strong>Evidence from included studies indicates that although PPIs are effective in healing esophagitis, they do not consistently improve esophageal motility, irrespective of the PPI type, dosage, or treatment duration.</p>","PeriodicalId":12636,"journal":{"name":"Gastroenterology and Hepatology From Bed to Bench","volume":"18 4","pages":"380-389"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12920681/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147348016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association between the Human Development Index and the burden of major gastrointestinal cancers: results from Global Burden of Disease 2021.","authors":"Mehdi Azizmohammad Looha, Ali Saberi Shahrbabaki, Sina Yoosefi, Sara Javadi, Mohamad Amin Pourhoseingholi, Amir Moradi","doi":"10.22037/ghfbb.v18i4.3203","DOIUrl":"10.22037/ghfbb.v18i4.3203","url":null,"abstract":"<p><strong>Aim: </strong>To assess the longitudinal association between the Human Development Index (HDI) and the global burden of major gastrointestinal (GI) cancers from 1990 to 2021.</p><p><strong>Background: </strong>GI cancers contribute substantially to global incidence, mortality, and disability-adjusted life years (DALYs), with wide variation across HDI levels.</p><p><strong>Methods: </strong>Using Global Burden of Disease 2021 data, we analyzed age-standardized incidence (ASIR), mortality (ASMR), disability-adjusted life-year (ASDR), and tobacco-attributable mortality for colorectal, liver, pancreatic, and stomach cancers (1990-2021). Countries were classified as more developed (HDI ≥0.700) or less developed (HDI <0.700), and associations were assessed using longitudinal multilevel mixed-effects models and Spearman correlations.</p><p><strong>Results: </strong>In 2021, HDI was positively correlated with colorectal and pancreatic cancer burden, while liver cancer showed inverse associations only in less developed countries and stomach cancer only in more developed settings. For tobacco-attributable ASMR, associations were positive for colorectal and pancreatic cancers, non-significant for liver, and opposite for stomach (positive in less vs. inverse in more developed countries). Longitudinally (1990-2021), colorectal cancer showed a rising ASIR in both groups, but significantly divergent slopes for ASMR and ASDR, with declining rates in more developed countries and increasing rates in less developed countries. Liver cancer had significantly unfavorable slopes, with ASIR, ASMR, and ASDR increasing in more developed but declining in less developed settings. Pancreatic cancer exhibited increases in all measures, with significantly slower growth in more developed countries. Stomach cancer showed consistent declines across all measures, with considerably steeper reductions in more developed regions.</p><p><strong>Conclusion: </strong>Human development strongly shapes GI cancer burden, with divergent high- and low-HDI trends evident only in longitudinal analyses.</p>","PeriodicalId":12636,"journal":{"name":"Gastroenterology and Hepatology From Bed to Bench","volume":"18 4","pages":"467-482"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12920687/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147348278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Design and optimization of a multiplex real-time PCR assay for detection of Hepatitis C virus (HCV), Human immunodeficiency virus-1 (HIV-1) and Human hepegivirus-1 (HHpgV-1).","authors":"Paria Pirasteh, Seyed Reza Mohebbi, Seyed Masoud Hosseini, Shabnam Kazemian, Amir Ghaemi, Abolfazl Fateh, Mohammad Reza Zali","doi":"10.22037/ghfbb.v18i3.3197","DOIUrl":"10.22037/ghfbb.v18i3.3197","url":null,"abstract":"<p><strong>Aim: </strong>The objective of this study was to develop a real-time PCR method to detect hepatitis C virus (HCV), human immunodeficiency virus-1 (HIV-1), and also recently discovered human hepegivirus-1 (HHpgV-1) through melting curve analysis using SYBR Green dye in a single reaction.</p><p><strong>Background: </strong>It is estimated that chronic viral infections affect millions of people worldwide, and a significant number of these individuals are unaware of their infection. In addition, infection with newly discovered and emerging viruses may put public health at potential risks. Precise and early detection is a primary and important task for controlling infections and helping patients.</p><p><strong>Methods: </strong>A set of specific primers were designed for detection of HCV, HIV-1, and HHpgV-1 in a single tube. The real-time polymerase chain reaction was performed and melt curves were analyzed. Specificity was assessed by cross-reaction tests with other common blood-borne pathogens. The established assay was evaluated for the detection of viruses in clinical samples.</p><p><strong>Results: </strong>The three viruses were clearly distinguished by their respective melting temperature values. The detection limits of this assay were 10² copies/ml for each virus. The clinical evaluation of this assay was demonstrated by analyzing 134 patients' serum samples. The specificity of the developed assay considered as 100%.</p><p><strong>Conclusion: </strong>The developed multiplex real-time PCR based on SYBR Green dye is a well-suited detection assay of single or co-infections of HCV, HIV, and HHpgV-1 in clinical and research laboratories. It can be used as a cost-effective, rapid tool for routine diagnostic in-house tests.</p>","PeriodicalId":12636,"journal":{"name":"Gastroenterology and Hepatology From Bed to Bench","volume":"18 3","pages":"353-360"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12535783/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145336765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}