Reproductive Immunology and Biology最新文献

{"title":"Ovarian dysfunction after ischemia-reperfusion of the ovarian blood vessel in experimental rats","authors":"K. Nariai, R. Fukumoto, Shin Onota, Ken Watanabe, H. Uchiyama, K. Kanayama, Kahei Sato","doi":"10.3192/JSIRIB.24.77","DOIUrl":"https://doi.org/10.3192/JSIRIB.24.77","url":null,"abstract":"Excessive production of reactive oxygen species (ROS) lead to oxidative stress in tissue or organ dysfunction. To confirm ovarian dysfunction by ROS, ischemia-reperfusion (I/R) treatment of the ovarian blood vessels was performed using experimental rats in this study. Preventive effect of superoxide dismutase (SOD) as a radical scavenger against ovarian I/R injury was also examined. Hormonal sensitivities to equine chorionic gonadotropin (eCG) and human chorionic gonadotropin (hCG) in the ovary decreased after the I/R. Ovarian tissue and ovum destruction were also observed. These changes could be prevented by SOD administration. Our data suggest that I/R injury related to ROS production causes ovarian dysfunction. The dysfunction is prevented by administration of a radical scavenger.","PeriodicalId":126001,"journal":{"name":"Reproductive Immunology and Biology","volume":"89 41 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129806853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"第20回 日本生殖免疫学会 学術集会抄録集","authors":"Lois A. Salamonsen","doi":"10.3192/JSIRIB2003.20.2_104","DOIUrl":"https://doi.org/10.3192/JSIRIB2003.20.2_104","url":null,"abstract":"","PeriodicalId":126001,"journal":{"name":"Reproductive Immunology and Biology","volume":"70 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124605337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"β2-グリコプロテインI/MHCクラスII複合体が抗リン脂質抗体症候群の病態に関連する","authors":"憲司 谷村, 康彦 蝦名, 達也 渥美, 秀人 山田, 尚 荒瀬","doi":"10.3192/JSIRIB.31.24","DOIUrl":"https://doi.org/10.3192/JSIRIB.31.24","url":null,"abstract":"","PeriodicalId":126001,"journal":{"name":"Reproductive Immunology and Biology","volume":"38 8","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"120921434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Maternal erythrocyte ENT1-mediated oxygen delivery is necessary for adequate placental oxygenation and fetal growth","authors":"S. Sayama, Anren Song, Benjamin C. Brown, J. Couturier, T. Iriyama, B. Sibai, R. Kellems, Angelo D’Alessandro, Yang Xia","doi":"10.3192/jsirib.35.24","DOIUrl":"https://doi.org/10.3192/jsirib.35.24","url":null,"abstract":"Background: Insufficient oxygen supply is closely associated with the pathophysiology of fetal growth restriction (FGR). Although the erythrocyte is the most abundant and only cell type to deliver oxygen in our body, its function and regulatory mechanism in FGR remains unknown. Recently, intracellular adenosine uptake by equilibrative nucleoside transporter 1 (ENT1), a key adenosine transporter predominantly expressed in erythrocytes, was reported to be crucial for erythrocytes to deliver oxygen. Current study was aimed to investigate the involvement of erythrocytes’ oxygen delivering capacity in maintaining fetal growth by focusing on erythrocyte ENT1. Methods and Results: Conditional knockout mice with erythrocyte-specific gene deletion of ENT1 were utilized in this study. These mice indeed showed reduction in oxygen delivering capacity during pregnancy compared to control mice. We found that genetic ablation of mouse erythrocyte ENT1 in dams results in FGR without showing any maternal features of preeclampsia. Unbiased highthroughput metabolic profiling led us to discover that these transgenic mice have lower amino acid concentration in the placenta and higher amino acid concentration in the serum compared to the control dams. Mechanistically and functionally, we revealed genetic ablation of maternal erythrocyte","PeriodicalId":126001,"journal":{"name":"Reproductive Immunology and Biology","volume":"6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121357827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Long-term organ culture of mouse fetal genital ridges","authors":"H. Motohashi, H. Kada, Kahei Sato","doi":"10.3192/JSIRIB2003.18.2_19","DOIUrl":"https://doi.org/10.3192/JSIRIB2003.18.2_19","url":null,"abstract":"We investigated the oocyte development in vitro from the genital ridges of 12.5 days post coitum (dpc) including premeiotic germ cells. The cultured tissues survived and the oocytes entered into growth phase. Mesonephric region with the genital ridge was vacuolated during culture in vitro. On day 18 of culture, follicle-like suructures had appeared in the cultured tissues. Morphology of oocytes isolated from the cultures was generally nomal, included the presence of a zona pellucida and had a nucleus of germinal vesicle (GV) stage. The oocyte diameter reached to 51.5 ± 0.5 pm. Stem cell factor (SCF) did not promote the oocyte growth in this culture system. The present work showed that the oocytes developed in vitro from the 12.5 dpc genital ridges reached to the midsize whether or not SCF is added to culture.","PeriodicalId":126001,"journal":{"name":"Reproductive Immunology and Biology","volume":"163 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127398706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effective Treatment for Recurrent Pregnancy Loss","authors":"T. Makino","doi":"10.3192/JSIRIB.25.1","DOIUrl":"https://doi.org/10.3192/JSIRIB.25.1","url":null,"abstract":"Understanding the etiology of recurrent pregnancy loss (RPL) has been increasing in importance in reproductive medicine, especially in societies with low birth rates. Since the cause of human miscarriage is often complicated and multifactorial, effective treatment for the successful maintenance of pregnancy requires the comprehensive clinical management of recurrent wastages. Immunology for the maintenance of pregnancy is a very attractive field; however, the role of immunology during human gestation cannot be confirmed in couples with a history of recurrent pregnancy loss unless other clinical causes of miscarriage are especially ruled out.Balanced chromosomal rearrangements have been found at an increased frequency in couples with recurrent pregnancy loss compared with the general population. The major anomalies are Robertosonian or reciprocal translocations, while most minor variants include pericentric inversion of chromosome 9. Both major and minor anomalies result in a spontaneous abortion rate of approximately 80-90%. Though the incidences of these chromosomal abnormalities vary in reported studies, a survey indicated an incidence of 9.8% (3.9% in male partners and 5.9% in female partners) among 1,639 couples with a history of recurrent pregnancy loss.Congenital uterine anomaly resulting in a suboptimal uterine environment may influence ovum implantation and early fetal development and may be a candidate in the etiology of reproductive wastage.A review on the relation between congenital uterine anomalies and the spontaneous abortion rate concluded that a precise diagnosis of uterine cavity deformity, especially arcuate uterus, is a key issue for determining the clinical prognosis during human gestation. After establishing diagnostic criteria for uterine cavity anomaly, 278 congenital uterine anomalies were detected in a series of 2,061 hysterosalpingographies (13.5%) performed in women with a history of recurrent pregnancy loss. Among these abnormalities, the incidences of spontaneous abortion were equally high among patients with arcuate uterus and those with partial septate uterus. A few studies on abortion mechanism in patients with congenital uterine anomalies have been reported. Histological findings using CD34- positive staining indicate the lack of a blood capillary system in both the uterine septum and the fundus muscle layer. The results of metroplastic surgery on congenital uterine anomaly are also controversial. Based on our fundamental findings of the maldistribution of uterine capillaries, we performed ideal metroplastic surgery in 173 women and significantly reduced the spontaneous abortion rate from a pre-operative values of 93.2% to a post-operative value of 12.5%.Anti-cardiolipin antibody was first described by Huge et al. as a pathogenic auto-antibody capable of causing recurrent pregnancy loss. Since then several other candidates for zwitterionic antiphospholipid antibodies to anionic phospholipids that are capable of in","PeriodicalId":126001,"journal":{"name":"Reproductive Immunology and Biology","volume":"7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131841817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of insulin-like growth factor II on preimplantation and post-blastocyst development in vitro of mouse parthenogenetic embryos","authors":"H. Kada, H. Motohashi, Kahei Sato","doi":"10.3192/JSIRIB2003.20.2_75","DOIUrl":"https://doi.org/10.3192/JSIRIB2003.20.2_75","url":null,"abstract":"Insulin-like growth factor ll (IGF-II) is first expressed from the zygotic genome at the two-cell stage of development in mouse embryo. However, the IGF-II is hardly expressed in parthenogenetic embryos because of paternal imprinted gene. In this work, addition of IGF-II (50 ng/ml) to culture medium for preimplantation and post-blastocyst development in vitro stimulated specifically proliferation of inner cell mass (ICM) cells, whereas no effect on mitosis of trophoectoderm (TE) was found. In addition, exogenous IGF-Il also contributed to prevent disappearance of ICM during the blastocyst outgrowth in vitro. These results indicate that IGF-II plays an important role for proliferation of ICM cells in not only fertilized embryos but also parthenogenetic embryos.","PeriodicalId":126001,"journal":{"name":"Reproductive Immunology and Biology","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130994332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The changes of the immune circumstance in the testicular tissues of mice treated with low dose of cadmium","authors":"Yuki Ogawa, N. Qu, Miyuki Kitaoka, M. Naito, H. Terayama, M. Itoh, Y. Matsuno, C. Mori","doi":"10.3192/JSIRIB.22.49","DOIUrl":"https://doi.org/10.3192/JSIRIB.22.49","url":null,"abstract":"精細管内のhaploid germ cellsは、様々な自己抗原を含むことがわかっているが、Sertoli細胞間の結合で構成される血液−精巣関門 (blood-testis barrier=BTB) によって免疫系 (精巣間質マクロファージを含む) からある程度守られているとされている。しかしながら、直精細管および精巣網には、投与された抗体やhorseradish peroxidase (HRP) が管内へ若干入り込むため、その部位のBTBは生理学的に脆弱なことが明らかとなってきている。精巣毒性物質として知られているカドミウム (CdCl2) は、主に精巣毛細血管障害を引き起こし、BTBの破壊や精上皮壊死を伴うことが報告されているが、本研究では、精子形成障害を惹起しない程度のCdCl2微量投与 (1mg/kgおよび3mg/kg) による精巣の免疫学的組織環境変化 (CdCl2投与12および72時間後の外来性HRPと内在性IgGの精巣内分布の変化) を、8週齢雄A/Jマウスを用いて組織化学的に検討した。その結果、CdCl2投与によって、HRP (屠殺30分前に投与) は直精細管・精巣網周囲の間質に強い集積を認めたが、間質の定住マクロファージによるHRP取り込みは精巣全域で逆に弱くなった。また、基底膜を超えて管内 (精細管→直精細管→精巣網) の上皮細胞に取り込まれたり上皮細胞間に侵入するHRP像を一部に認めたが、特に直精細管・精巣網に選択的に起こるものでなかった。一方、内在性のIgGは、HRPのような局在性分布や定住マクロファージによる取り込みを、対照群、CdCl2投与群ともに認めず、精細管→直精細管→精巣網への侵入像も精細管の一部に観察するのみであった。よって、精子形成障害を引き起こさない程度の微量CdCl2投与によっても、精巣間質における微小循環やマクロファージの機能に障害が起こることが示唆された。また、明らかなBTBの破綻とは異なる精細管→直精細管→精巣網の基底膜の一部に破壊が起こることが示唆された。しかし、その破壊は生理学的にBTBが脆弱とされる直精細管・精巣網に強く起こるということはないことがわかった。","PeriodicalId":126001,"journal":{"name":"Reproductive Immunology and Biology","volume":"40 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116131175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of autophagy in pregnancy","authors":"A. Nakashima, Tae Kusabiraki, Aiko Aoki, T. Shima, Azusa Sameshima, Kumiko Inada, O. Yoshino, S. Saito","doi":"10.3192/JSIRIB.31.9","DOIUrl":"https://doi.org/10.3192/JSIRIB.31.9","url":null,"abstract":"Autophagy is an evolutionarily conserved process in eukaryotes, by which cytoplasmic cargo sequestered inside double-membrane vesicles are delivered to the lysosome for degradation. In early pregnancy, trophoblasts and the fetus experience hypoxic and low-nutrient conditions; nevertheless, extravillous trophoblasts (EVTs) invade the uterine myometrium up to one third of its depth and migrate along the lumina of spiral arterioles, replacing the maternal endothelial lining. An enhancement of autophagy induced by physiological hypoxia takes part in the invasion and vascular remodeling in EVTs. On the other hand, soluble endoglin, which increased in sera in preeclamptic cases, suppressed EVTinvasion or -vascular remodeling by inhibiting autophagy in vitro. In addition, a substance selectively degraded by autophagy, p62/SQSTM1, accumulated in EVT cells in preeclamptic placental bed biopsy samples showing impaired autophagy in vivo. There is, however, still a conflict in state of autophagy in preeclamptic placentas. In this paper, we review the importance of autophagy inhibition for placentation, and propose the future direction of autophagy in placenta.","PeriodicalId":126001,"journal":{"name":"Reproductive Immunology and Biology","volume":"84 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122970111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pursuing the identity of Super-early pregnancy factor","authors":"Kota Tokunaka, H. Kamada, Kazuei Matsubara-Ito","doi":"10.3192/JSIRIB.25.14","DOIUrl":"https://doi.org/10.3192/JSIRIB.25.14","url":null,"abstract":"In 1974, Morton et al. found that substance(s) included in the pregnancy mouse serum inhibited the rosette formation and named it “early pregnancy factor(EPF)”. Such inhibition is thought to be caused by an immunosuppressive property of EPF, and investigation of this factor could be the key to interpreting the immunological relationship between mother and fetus during pregnancy. From our extensive studies of EPF, we have defined Super-EPF as that detected in the mammalian pregnancy serum and EPF-like substance(s) as those detected in other materials. Even 35 years have passed since the discovery of EPF, little is known about this substance. In this review, we mainly describe our data obtained from purification for determine the structure of bovine Super-EPF and EPF-like substance(s). We also discuss the characteristics and potential of SuperEPF based on our findings.","PeriodicalId":126001,"journal":{"name":"Reproductive Immunology and Biology","volume":"54 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121442951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}