Reproductive Immunology and Biology最新文献_第6页

Contribution of zona pellucida proteins to ovarian folliculogenesis 透明带蛋白对卵巢卵泡发生的贡献

Reproductive Immunology and Biology Pub Date : 1900-01-01 DOI: 10.3192/JSIRIB.22.1

A. Hasegawa, Nozomi Kanazawa, K. Koyama

引用次数: 2

Abstracts of the 19th Annual Meeting of Japan Society for Immunology of Reproduction 日本生殖免疫学学会第19届年会摘要

Reproductive Immunology and Biology Pub Date : 1900-01-01 DOI: 10.3192/JSIRIB2003.20.1_33

de Yama, Hakone, Kanagawa

引用次数: 0

Alteration of DC subsets and kinetics of serum IL-12 during pregnancy 妊娠期DC亚群的改变和血清IL-12的动力学

Reproductive Immunology and Biology Pub Date : 1900-01-01 DOI: 10.3192/JSIRIB.28.26

Yasuyuki Negishi, Masumi Shimizu, A. Wakabayashi, T. Ichikawa, T. Takeshita, Hidemi Takahashi

{"title":"Alteration of DC subsets and kinetics of serum IL-12 during pregnancy","authors":"Yasuyuki Negishi, Masumi Shimizu, A. Wakabayashi, T. Ichikawa, T. Takeshita, Hidemi Takahashi","doi":"10.3192/JSIRIB.28.26","DOIUrl":"https://doi.org/10.3192/JSIRIB.28.26","url":null,"abstract":"Dendritic cells (DCs) play a crucial role in providing an appropriate immune balance during pregnancy. It has recently been reported that two distinct types of DCs are arranged in the murine system. One subtype is the DCs bearing the C-type lectin CD205 (DEC-205) having the capacity to establish Th1 polarization. The other is the DCs expressing 33D1, recognizing DC inhibitory receptor-2 (DCIR-2), having the capacity to induce Th2 polarization. In addition, we found that the balance of DC subtypes was aff ected mainly by progesterone, which induced a dose-dependent reduction of the DEC-205/33D1 ratio together with/without a stable amount of estrogen(1). The DEC-205/33D1 ratio decreased gradually with the progress of pregnancy and rapid augmentation of this ratio was seen around delivery period. Here, we demonstrated that the fetal loss could be induced by the depletion of 33D1+ DCs during perinatal period mediated through the transient IL-12 secretion, and pre-administration of progesterone could rescue this fetal loss. Similar miscarriages were also observed when pregnant mice were intraperitoneally (i.p.) injected twice with IL-12 on Gd 9.5 and 10.5. Moreover, prior inoculation of progesterone suppressed the enhanced serum IL-12 production in mice treated with 33D1 antibody, indicating that progesterone might inhibit temporal IL-12 secretion around Gd 10.5 and thus miscarriage was prevented. These findings suggest that the balance of DC subsets is crucial for maintaining pregnancy and we can prevent miscarriage by manipulating the activity of the DC subpopulation of pregnant individuals by progesterone administration. 根岸靖幸 a),b)、清水真澄 a)、若林あや子 a)、市川智子 a),b)、竹下俊行 b)、高橋秀実 a) Yasuyuki Negishi, Masumi Shimizu, Ayako Wakabayashi, Tomoko Ichikawa, Toshiyuki Takeshita, Hidemi Takahashi","PeriodicalId":126001,"journal":{"name":"Reproductive Immunology and Biology","volume":"9 9","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132417873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Vitrification for embryo and oocyte freezing 胚胎和卵母细胞的玻璃化冷冻

Reproductive Immunology and Biology Pub Date : 1900-01-01 DOI: 10.3192/JSIRIB2003.20.1_13

H. Saito

{"title":"Vitrification for embryo and oocyte freezing","authors":"H. Saito","doi":"10.3192/JSIRIB2003.20.1_13","DOIUrl":"https://doi.org/10.3192/JSIRIB2003.20.1_13","url":null,"abstract":"In vitro fertilization and embryo transfer (IVF-ET) techniques have improved and more pregnancies have been established associated with higher rates of multiple pregnancies. Multiple pregnancies are associated with various obstetric complications including threatened abortion, threatened premature delivery, fetal malformation, necessity of cervical ligation and caesarean section, increased uterine bleeding at birth, and low birth weight babies. To prevent multiple pregnancies, one of the best approaches is to decrease the number of embryos transferred into the uterus. Consequently, several embryos are left to be cryopreserved for future embryo transfer. Since Whittingham [1] first succeeded in freezing and thawing mouse embryos, different cryoprotectants, and freezing and thawing techniques have been investigated to improve the method. According to Whittingham's freezing system, 2 to 3 hours in the cryoprotectant are necessary for embryos to be frozen with minimal ice formation in the blastomeres. To avoid the lethal effects of ice formation, Luyte drew attention to the feasibility of using vitrification to cryopreserve biological materials [2]. Rall and Fahy [3] applied the vitrification method to cryopreserve mouse embryos using dimethyl sulfoxide (DMSO), acetamide, propylene glycol and polyethylene glycol. Unfortunately, when embryos were exposed for a long time at room temperature, they were adversely affected by the cryoprotectant at the concentration needed to vitrify embryos. Therefore, to achieve adequate vitrification, it was necessary a very short period of time to equilibrate embryo with the cryoprotectant. As the initial vitrification solutions were very toxic [3] to embryos, it was essential to formulate nontoxic and more efficient solutions for vitrification. Kasai developed a less toxic cryoprotectant composed of modified phosphate buffer solution (PBS), ethylene glycol, ficoll and sucrose [4]. He showed that the optimal equilibration time in the vitrification solution before rapid cooling varied with the ambient temperature when using the rate of blastocyst formation as a criterion [5]. Since then, other investigators developed more effective modern solutions that contained nonpenetrating additives [6-11], and various vitrification methods have been used for freezing human embryos [12-14]. Vitrification is a short and simple procedure that may be safer and more cost effective than slow cooling for embryo freezing. Kuleshova et al. compared the advantages and disadvantages of slow cooling and vitrification. Their results are shown in Table 1 [15]. Generally, the ultrastructure of oocytes is particularly sensitive to temperature and extracellular osmotic pressure changes during freezing and thawing. Various types of cellular damage are observed during oocyte freezing [16-18]. However, a reasonable number of deliveries have been achieved with the use of vitrified oocytes (Table 2). Although vitrification is still in its early stage o","PeriodicalId":126001,"journal":{"name":"Reproductive Immunology and Biology","volume":"37 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124091788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antiphospholipid antibody and pregnancy 抗磷脂抗体与妊娠

Reproductive Immunology and Biology Pub Date : 1900-01-01 DOI: 10.3192/JSIRIB.28.11

Tatsuo Yamamoto, F. Chishima, Hiromitsu Azuma, Akikazu Nakamura, G. Ichikawa, Shigetane Sasaki, Suzuki Manami

引用次数: 2

Effects of Environmental Chemicals on Cell Division and Chromosomal Positioning 环境化学物质对细胞分裂和染色体定位的影响

Reproductive Immunology and Biology Pub Date : 1900-01-01 DOI: 10.3192/JSIRIB.24.70

K. Oikawa, Y. Matsuda, M. Kuroda

引用次数: 0