Reproductive Immunology and Biology最新文献

{"title":"Epstein-Barr virus-Induced gene-3(EBI3)knockoutマウスにおける 精巣内免疫微小環境の変化","authors":"隼人 寺山, 宗一 平井, 宗和 内藤, 直之 畑山, 省吾 林, 隆之 善本, 貢 坂部, 正裕 伊藤","doi":"10.3192/JSIRIB.28.19","DOIUrl":"https://doi.org/10.3192/JSIRIB.28.19","url":null,"abstract":"","PeriodicalId":126001,"journal":{"name":"Reproductive Immunology and Biology","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132954403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Ovariectomy and Estrogen Treatment on Diabetic Pathogenesis in Spontaneously Diabetic Torii (SDT) Rats","authors":"M. Shinohara, T. Oikawa, Kahei Sato","doi":"10.3192/JSIRIB2003.20.1_5","DOIUrl":"https://doi.org/10.3192/JSIRIB2003.20.1_5","url":null,"abstract":"","PeriodicalId":126001,"journal":{"name":"Reproductive Immunology and Biology","volume":"43 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129181020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of assisted reproductive technology on multiple pregnancy","authors":"M. Matsumoto","doi":"10.3192/JSIRIB2003.20.1_24","DOIUrl":"https://doi.org/10.3192/JSIRIB2003.20.1_24","url":null,"abstract":"With the recent spread of assisted reproductive technology (ART), about 1% of newborns in Japan are born by ART [1], and the incidence of multiple pregnancy is increasing. Compared with single pregnancy, multiple pregnancy shows a high incidence of maternal complications and premature deliveries and is an important problem in perinatal medicine [2]. Among multiple pregnancies, super twins (triplets or more) and monochorionic multiple pregnancy have particularly high risks. Super twins have been conventionally managed by limiting the number of transferred embryos. After introduction of blastocyst transfer with high selectivity of good embryos, the incidence of monochorionic multiple pregnancy has been increasing. We report the association between ART and multiple pregnancy, especially, the prevention of multiple pregnancy by limiting the number of transferred embryos, the transfer time, and monochorionic multiple pregnancy.","PeriodicalId":126001,"journal":{"name":"Reproductive Immunology and Biology","volume":"63 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131676968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The cell to cell interaction between peritoneal macrophages and endometrial stromal cells via Stat3 activation in human endometriosis","authors":"F. Itoh, Y. Komohara, K. Takaishi, R. Honda, H. Tashiro, M. Takeya, H. Katabuchi","doi":"10.3192/JSIRIB.29.24","DOIUrl":"https://doi.org/10.3192/JSIRIB.29.24","url":null,"abstract":"Objectives: Endometriosis frequently develops in the female pelvic cavity. This suggests that the peritoneal environment that contains various free-fl oating cells such as macrophages and endometrial cells derived from refl uxed menstrual blood may contribute to the development of endometriosis. We previously reported that the proportion of active macrophages was elevated in ascites from patients with endometriosis, and in another study, activated macrophages reportedly released several cytokines into the peritoneal cavity. This study focused on the interactions between macrophages and endometrial stromal cells (ESCs) in the development of endometriosis. Methods: The phenotype of macrophages in the ascites from patients with endometriosis was determined by immunocytochemistry. Soluble factors in ascites and conditioned medium from human macrophages and ESCs in co-culture system were quantified by the Cytokine Array Kit. Next, we investigated the significance of macrophages for proliferation and Stat3 activation of ESCs by using co-cultured cells. Furthermore, pSTAT3 expression of peritoneal foci of endometriosis was assessed by immunohistochemistry. Finally, the effects of corosolic acid (CA), a triterpenoid compound, on proliferation and Stat3 activation of ESCs in co-cultures were evaluated. Results: The total number of CD163+ macrophages (M2 macrophages) in ascites from the endometriosis group was signifi cantly higher than that of the control group. The cytokine array study detected that the production of GM-CSF, RANTES, IL-1 receptor antagonist, and MCP-1 were up-regulated in ascites from patients with endometriosis and in conditioned medium from co-cultured cells (c-CM). Coculture with M2 macrophages signifi cantly up-regulated ESC proliferation and Stat3 activation in ESCs in vitro. Immunohistochemically, Stat3 was activated in both epithelial and stromal cells in endometriotic lesions. When the inhibitory eff ect of a Stat3 inhibitor, CA on ESC was investigated, CA inhibited ESC proliferation and Stat3 activation induced by c-CM. Conclusions: These fi ndings indicated that the interactions between M2 macrophages and ESCs via Stat3 activation may play indispensable roles in the development of endometriosis. Targeting Stat3 signals may aid the treatment of patients with endometriosis.","PeriodicalId":126001,"journal":{"name":"Reproductive Immunology and Biology","volume":"29 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131206629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of NLRP3 inflammasome on preeclampsia","authors":"K. Shirasuna, Hironori Takahashi, A. Ohkuchi","doi":"10.3192/jsirib.34.1","DOIUrl":"https://doi.org/10.3192/jsirib.34.1","url":null,"abstract":"","PeriodicalId":126001,"journal":{"name":"Reproductive Immunology and Biology","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116627321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bio-nanocapsule(BNC)を用いた子宮局所への新しいdrug delivery systemの構築","authors":"花織 小泉, 仁美 中村, 高志 松崎, 俊一 黒田, 剛 瀧内, 恵一 熊澤, 正 木村","doi":"10.3192/JSIRIB.30.13","DOIUrl":"https://doi.org/10.3192/JSIRIB.30.13","url":null,"abstract":"","PeriodicalId":126001,"journal":{"name":"Reproductive Immunology and Biology","volume":"580 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122763225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Understanding the Molecular Basis of-“The Window of Implantation”","authors":"C. Stewart","doi":"10.3192/JSIRIB2003.18.2_45","DOIUrl":"https://doi.org/10.3192/JSIRIB2003.18.2_45","url":null,"abstract":"","PeriodicalId":126001,"journal":{"name":"Reproductive Immunology and Biology","volume":"204 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132163355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of fertilization and developmental ability of oocytes injected with spermatids derived from three strains in mice","authors":"Y. Sato, Kahei Sato","doi":"10.3192/JSIRIB.22.8","DOIUrl":"https://doi.org/10.3192/JSIRIB.22.8","url":null,"abstract":"Recently, the spermatogenic cell has been focused in male sterility. There are some reports on fertilization and embryonic developmental ability of spermatid. Deficiency of fertilization or developmental arrest frequently appears due to the ability. Functional immaturity is also observed in spite of abnormality of karyotype in spermatids. However, a functional difference between spermatids and spermatozoa is not clarified. In this study, fertilization and developmental ability of the spermatid were examined by the microinjection using immature spermatid including the round spermatid, elongating spermatid, elongated spermatid and intratesticular spermatozoa derived from three different mice strains; ddY, ICR and B6D2F1. Mature female mice were superovulated with PMSG and hCG. Cumulus-oosytes complex were obtained from their oviducts, and cumulus cells were removed from MII oocytes by hyaluronidase and pippeting. Spermatids were collected from mature male testis, and were injected into MII oocytes using a micromanipulator. Manipulated embryos were cultured in CZB medium, and their ability for development was examined. Epididymal spermatozoa from the three strains were used as control group. The fertilization rate was correlated with the spermatogenic stage. In embryonic developmental rate, on the other hand, there were no significant difference between the control and experimental embryos. The data however showed that elongating, elongated and round spermatid slightly deteriorated the embryonic development. These results suggest that the ability of the embryonic activation depend on the spermatid metamorphose.","PeriodicalId":126001,"journal":{"name":"Reproductive Immunology and Biology","volume":"59 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130927526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Co-evolution of viviparity and acquired immune system—why sharks got the both","authors":"S. Hayakawa, S. Komine-Aizawa, K. Asahina","doi":"10.3192/JSIRIB.29.1","DOIUrl":"https://doi.org/10.3192/JSIRIB.29.1","url":null,"abstract":"","PeriodicalId":126001,"journal":{"name":"Reproductive Immunology and Biology","volume":"105 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116907450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serum levels of midkine, a heparin-binding growth factor, increase in both malignant and benign gynecological tumors","authors":"R. Salama, H. Muramatsu, Honami Kobayashi, S. Nomura, S. Mizutani, T. Muramatsu","doi":"10.3192/JSIRIB.21.64","DOIUrl":"https://doi.org/10.3192/JSIRIB.21.64","url":null,"abstract":"Midkine (MK) is a heparin-binding growth factor, the expression of which is strong in the midgestation period, is highly restricted in the adult, and is increased in many human carcinomas. In the present investigation, we determined serum levels of MK by an enzyme immunoassay, and found that in many patients with gynecological tumors, the values increased above the cut off value of 500 pg/ml. All control subjects (n=49) including pregnant women exhibited values not more than 500 pg/ml. The percentage of positive cases was as follows; ovarian carcinoma (n=15), 87%; uterine cervical carcinoma (n=18), 67%; uterine endometrial carcinoma (n=10), 50%; ovarian cyst (n=25), 72%; leiomyoma (n=29), 72%. CA-125 and MK levels were not correlated in ovarian carcinoma and ovarian cyst; a combination of the two assays may be helpful in increasing specificity or sensitivity. Furthermore, increased MK levels were found even in patients with early stages of uterine cervical and endometrial carcinomas. Serum MK levels might become helpful in the initial screening of gynecological tumors.","PeriodicalId":126001,"journal":{"name":"Reproductive Immunology and Biology","volume":"118 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115385193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}