General and comparative endocrinology最新文献

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Effects of dietary quality on melatonin in gut, serum and ovary in mediating growth, digestive and ovarian physiology in juvenile carp (Catla catla) 饲料品质对幼鱼肠道、血清和卵巢褪黑素在调节幼鱼生长、消化和卵巢生理中的影响。
IF 2.1 3区 医学
General and comparative endocrinology Pub Date : 2025-05-17 DOI: 10.1016/j.ygcen.2025.114752
Farha Yasmin, Sona Sutradhar, Arun Roy, Sourav Mukherjee
{"title":"Effects of dietary quality on melatonin in gut, serum and ovary in mediating growth, digestive and ovarian physiology in juvenile carp (Catla catla)","authors":"Farha Yasmin,&nbsp;Sona Sutradhar,&nbsp;Arun Roy,&nbsp;Sourav Mukherjee","doi":"10.1016/j.ygcen.2025.114752","DOIUrl":"10.1016/j.ygcen.2025.114752","url":null,"abstract":"<div><div>The dietary protein and tryptophan of fish feed have vital roles in the growth performance, stress management, and digestive physiology of fish. However, in this context, the functions of gut melatonin, which depend on the availability of food, the timing of food supply, the frequency of feeds/day, the quality of food, and the growth stages of carp, still need to be clarified. Hence, the present study aimed to investigate the impact of dietary quality on specific growth rate (SGR), residual gut content (RGC), feeding intensity (FI), gut melatonin, gut oxidative stress and its major antioxidants, vital digestive enzymes in juvenile carp, <em>Catla catla</em> (mean body weight: 274 ± 16.63 g). Besides, serum melatonin and ovarian melatonin, gonado-somatic index (GSI), and different growth stages of oocytes were also assessed before the estimation of oxidative stress and major antioxidant enzymes in the ovary. The fish were fed four diets viz. (i) a standard diet (SD/control) (with 34.99 % protein, 14.56 % carbohydrate, 9.84 % oil, and 0.36 % L-tryptophan) (ii) one protein (PRD with 41.02 % protein and 0.46 % L-tryptophan), (iii) two L-tryptophan (TrpRD1 with 0.96 %, and TrpRD2 with 1.36 % tryptophan), -rich diets for 30 days. The SGR was higher only in PRD, but feeding was reduced in PRD and TrpRDs compared to SD-fed carp. However, the PRD and TrpRDs stimulated gut melatonin, digestive enzymes and all the studied antioxidants with reduced oxidative stress by lowering malondialdehyde (MDA). Moreover, a parallel increase in serum melatonin and ovarian melatonin with gut melatonin has been noticed in both the PRD and TrpRDs. All these experimental diets showed pro-gonadal effects by increasing stage-I oocytes and by reducing oxidative stress in the developing ovary in juvenile carp, compared to SD-fed carp. This opens avenues for future research on the role of feed-induced gut melatonin in fish nutrition and reproduction.</div></div>","PeriodicalId":12582,"journal":{"name":"General and comparative endocrinology","volume":"369 ","pages":"Article 114752"},"PeriodicalIF":2.1,"publicationDate":"2025-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144101602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
GLP-1 receptor agonist properties of a chimeric peptide derived by hybridization of Latrodectus αLatrotoxin and Heloderma Exendin-4 Latrodectus αLatrotoxin与Heloderma Exendin-4杂交获得的嵌合肽GLP-1受体激动剂特性
IF 2.1 3区 医学
General and comparative endocrinology Pub Date : 2025-05-08 DOI: 10.1016/j.ygcen.2025.114745
Oleg G. Chepurny , Amber N. Liles , Nancy Cham , Minos-Timotheos Matsoukas , George Liapakis , Qinghe Meng , Robert N. Cooney , Robert P. Doyle , George G. Holz
{"title":"GLP-1 receptor agonist properties of a chimeric peptide derived by hybridization of Latrodectus αLatrotoxin and Heloderma Exendin-4","authors":"Oleg G. Chepurny ,&nbsp;Amber N. Liles ,&nbsp;Nancy Cham ,&nbsp;Minos-Timotheos Matsoukas ,&nbsp;George Liapakis ,&nbsp;Qinghe Meng ,&nbsp;Robert N. Cooney ,&nbsp;Robert P. Doyle ,&nbsp;George G. Holz","doi":"10.1016/j.ygcen.2025.114745","DOIUrl":"10.1016/j.ygcen.2025.114745","url":null,"abstract":"<div><div>Chimeric peptides comprised of amino acid sequence motifs found within hormones, neuropeptides, and insect or lizard toxins are now under investigation for their potential use in therapeutics. Here, we report the discovery of one such peptide designated as Black Widow Spider-Exendin-4 (BW-Ex-4). It consists of a putative G protein-coupled receptor (GPCR) binding domain present within αLatrotoxin (αLTX) isolated from <em>Latrodectus</em>, and fused to N- and C- terminal motifs found within the glucagon-like peptide-1 receptor (GLP-1R) agonist Exendin-4 isolated from <em>Heloderma</em>. FRET reporter assays that monitor cAMP production establish BW-Ex-4 to be a specific GLP-1R agonist without any stimulatory action at glucose-dependent insulinotropic peptide (GIP), glucagon, or corticotropin releasing hormone (CRH) receptors. Structural modeling studies of the predicted BW-Ex-4 binding sites at GPCRs of Family B provide new insights concerning the molecular basis for chimeric peptide stimulatory actions at the GLP-1R. We also report that BW-Ex-4 acts in obese hyperglycemic <em>Lepr<sup>db/db</sup></em> mice to suppress appetite, lower body weight, improve glucoregulation, and to reduce circulating levels of pro-inflammatory cytokines. Collectively, these findings establish a combinatorial chimeric peptide chemistry in which αLTX serves as a molecular scaffold for the design of hybrid peptides with novel GPCR stimulating properties.</div></div>","PeriodicalId":12582,"journal":{"name":"General and comparative endocrinology","volume":"368 ","pages":"Article 114745"},"PeriodicalIF":2.1,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143937555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
In vivo effects of NPFF and NPAF peptides on gene expression in the brain-pituitary–gonadal axis of female half-smooth tongue soles NPFF和NPAF肽对雌性半光滑舌底脑-垂体-性腺轴基因表达的体内影响
IF 2.1 3区 医学
General and comparative endocrinology Pub Date : 2025-04-21 DOI: 10.1016/j.ygcen.2025.114736
Hanlin Liu , Ruixue Li , Zhihua Yu , Huapu Chen , Bin Wang
{"title":"In vivo effects of NPFF and NPAF peptides on gene expression in the brain-pituitary–gonadal axis of female half-smooth tongue soles","authors":"Hanlin Liu ,&nbsp;Ruixue Li ,&nbsp;Zhihua Yu ,&nbsp;Huapu Chen ,&nbsp;Bin Wang","doi":"10.1016/j.ygcen.2025.114736","DOIUrl":"10.1016/j.ygcen.2025.114736","url":null,"abstract":"<div><div>Neuropeptide FF (NPFF) is one of the members of RF-amide peptide family, which has been implicated in diverse physiological processes across mammals. However, there is scarce information regarding the biological function of NPFF in fish reproduction. In the current study, <em>npff</em> was identified and characterized in the half-smooth tongue sole. The NPFF precursor consisted of one signal peptide and two mature peptides, designated as NPFF and NPAF. Analysis of tissue distribution indicated that <em>npff</em> exhibited high expression levels in the brain and pituitary, while its mRNA levels were much lower in other peripheral tissues. Subsequently, the possible function of NPFF and NPAF on the brain-pituitary-gonad reproductive axis was studied in this species by intraperitoneal injection. At the brain level, both NPFF and NPAF had an inhibitory influence on the levels of transcripts corresponding to <em>kiss1</em>, <em>kiss1r</em>, <em>kiss2</em>, <em>gnrh3</em>, <em>spx1</em>, <em>pnx</em>, <em>gpr173a</em>, and <em>gpr173b</em>. NPFF reduced <em>gnrh2</em> mRNA levels, but promoted <em>gnihr</em> expression. Moreover, NPAF down-regulated <em>gnih</em> and <em>tac3</em> expression. At the pituitary levels, <em>gh</em>, <em>gthα</em>, and <em>fshβ</em> mRNA levels were suppressed by NPFF and NPAF, whereas <em>lhβ</em> mRNA levels were elevated. In the ovary, these two peptides stimulated expression of <em>star</em>, <em>p450c17</em>, and <em>fshr</em>. Likewise, NPFF increased <em>17β-hsd</em> and <em>lhr</em> mRNA levels, and NPAF stimulated <em>p450arom</em> transcript levels. However, <em>3β-hsd</em> was inhibited by NPFF. Overall, our results suggest that NPFF and NPAF may play distinct roles in control of reproduction of half-smooth tongue sole at multiple levels of the brain-pituitary-ovarian axis.</div></div>","PeriodicalId":12582,"journal":{"name":"General and comparative endocrinology","volume":"368 ","pages":"Article 114736"},"PeriodicalIF":2.1,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143863348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Characterization of a direct role of GnRHs in the control of spermiogenesis and steroidogenesis in the small-spotted catshark Scyliorhinus canicula GnRHs在小斑点猫鲨(scylliorhinus canicula)精子发生和甾体发生控制中的直接作用
IF 2.1 3区 医学
General and comparative endocrinology Pub Date : 2025-04-18 DOI: 10.1016/j.ygcen.2025.114734
Fabian Jeanne , Stanislas Pilet , Benoît Bernay , Stéphanie Lagadu , Raphaël Delépée , Sylvie Dufour , Pascal Sourdaine
{"title":"Characterization of a direct role of GnRHs in the control of spermiogenesis and steroidogenesis in the small-spotted catshark Scyliorhinus canicula","authors":"Fabian Jeanne ,&nbsp;Stanislas Pilet ,&nbsp;Benoît Bernay ,&nbsp;Stéphanie Lagadu ,&nbsp;Raphaël Delépée ,&nbsp;Sylvie Dufour ,&nbsp;Pascal Sourdaine","doi":"10.1016/j.ygcen.2025.114734","DOIUrl":"10.1016/j.ygcen.2025.114734","url":null,"abstract":"<div><div>Hypothalamic gonadotropin-releasing hormone (GnRH) regulates the production of gonadotropins, which control reproduction. In elasmobranchs, unlike other gnathostomes, GnRH is released into the systemic circulation to stimulate gonadotrope cells located in the ventral lobe of the pituitary. The aim of this study was to investigate the potential role of systemic GnRH in the regulation of the testis in <em>Scyliorhinus canicula.</em> Phylogeny and synteny analyses identified three GnRHs and four GnRH receptor (<em>Sc</em>GnRHR-I1, -IIa1, -IIa2 and -IIb2). <em>In vitro</em> functional hormone-receptor interactions using synthetic <em>Sc</em>GnRHs showed that all <em>Sc</em>GnRHs were effective at receptors, except ScGnRHRIIa2, at femtomolar to nanomolar concentrations, with lower efficiency for <em>Sc</em>GnRH1/<em>Sc</em>GnRHRIIb2. Real-time PCR analyses in a wide range of tissues, including male and female reproductive tracts, showed that all three <em>gnrh</em> were expressed mainly in the brain and all four <em>gnrhr</em> were expressed in the testis, particularly during spermiogenesis. Testicular explants containing cysts with spermatids were treated with <em>Sc</em>GnRHs and their protein content analyzed by NanoLC-ESI-MS/MS, highlighting 1677 significantly differentially expressed proteins. Among them, the growth hormone receptor (GHR) and proteins involved in cholesterol and steroid metabolism, including several HSD17bs, were upregulated. <em>In situ</em> hybridization showed that <em>ghr</em>, <em>hsd17b3</em> and <em>hsd17b12</em> transcripts were localized in Sertoli cells, which are the main testicular steroidogenic cells in <em>S. canicula</em>. Fifteen steroids were assayed in the culture media, using LC-ESI-HRMS/MS, and an increase in 17β-estradiol concentrations was observed, consistent with <em>hsd17b</em> expressions. Furthermore, proteins involved in transcription and DNA structure were downregulated in response to GnRHs. In conclusion, this study showed that <em>Sc</em>GnRHs may play a direct role in the regulation of elasmobranch testes by promoting spermiogenesis and modulating steroidogenesis.</div></div>","PeriodicalId":12582,"journal":{"name":"General and comparative endocrinology","volume":"368 ","pages":"Article 114734"},"PeriodicalIF":2.1,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143874425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The distribution of gonadotropin-inhibitory hormone (GnIH) and its receptor in zebra finch (Taeniopygia guttata) spinal cord 促性腺激素抑制激素(GnIH)及其受体在斑胸草雀脊髓中的分布
IF 2.1 3区 医学
General and comparative endocrinology Pub Date : 2025-04-17 DOI: 10.1016/j.ygcen.2025.114733
Devon Comito, George E. Bentley
{"title":"The distribution of gonadotropin-inhibitory hormone (GnIH) and its receptor in zebra finch (Taeniopygia guttata) spinal cord","authors":"Devon Comito,&nbsp;George E. Bentley","doi":"10.1016/j.ygcen.2025.114733","DOIUrl":"10.1016/j.ygcen.2025.114733","url":null,"abstract":"<div><div>Gonadotropin-inhibitory hormone (GnIH) is a neuropeptide that typically acts in the hypothalamic-pituitary–gonadal (HPG) axis to regulate reproductive activity and sociosexual behaviors. GnIH is synthesized in the brain and in the gonads, where it can act via its cognate receptor. However, immunohistological evidence in songbirds also shows GnIH projections towards the brainstem. We propose that GnIH can act within the spinal cord and possibly on a variety of organs to induce rapid behavioral and physiological changes in response to environmental cues. Here we used immunohistochemistry (IHC) and PCR to document GnIH and its receptor in zebra finch (<em>Taeniopygia guttata</em>) spinal cord. We found immunoreactive GnIH throughout the length of the spine, predominantly in the central gray matter of the cervical region. mRNA for the GnIH precursor gene and receptor gene was also present throughout the length of the spinal cord. This is the first evidence of GnIH in the avian spinal cord. These results hint at a novel pathway for neuropeptide action in vertebrates.</div></div>","PeriodicalId":12582,"journal":{"name":"General and comparative endocrinology","volume":"368 ","pages":"Article 114733"},"PeriodicalIF":2.1,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143908288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Intelectin-1 promotes granulosa cells proliferation and modulates apoptosis via ERK1/2, AKT, and insulin receptor signaling pathways in Large White and Meishan pigs 白细胞介素-1通过ERK1/2、AKT和胰岛素受体信号通路促进大白猪和梅山猪颗粒细胞增殖并调节细胞凋亡
IF 2.1 3区 医学
General and comparative endocrinology Pub Date : 2025-04-16 DOI: 10.1016/j.ygcen.2025.114722
Karolina Pich , Natalia Respekta-Długosz , Patrycja Kurowska , Małgorzata Opydo , Nina Smolińska , Joëlle Dupont , Agnieszka Rak
{"title":"Intelectin-1 promotes granulosa cells proliferation and modulates apoptosis via ERK1/2, AKT, and insulin receptor signaling pathways in Large White and Meishan pigs","authors":"Karolina Pich ,&nbsp;Natalia Respekta-Długosz ,&nbsp;Patrycja Kurowska ,&nbsp;Małgorzata Opydo ,&nbsp;Nina Smolińska ,&nbsp;Joëlle Dupont ,&nbsp;Agnieszka Rak","doi":"10.1016/j.ygcen.2025.114722","DOIUrl":"10.1016/j.ygcen.2025.114722","url":null,"abstract":"<div><div>Maintaining the proper balance between granulosa cells (Gc) proliferation and apoptosis is crucial for folliculogenesis and female fertility. Our previous study showed expression of omentin-1 (intelectin-1, ITLN1) in the porcine ovarian follicles; however, its impact on Gc functions remains unknown. Therefore, this study aimed to determine the <em>in vitro</em> effects of ITLN1 on Gc proliferation and apoptosis in Large White (LW) and Meishan (MS) pigs. These breeds were chosen due to their distinct reproductive characteristics: MS pigs are known for maintaining a higher number of follicles during the follicular phase and exhibiting greater estradiol synthesis compared to LW pigs. Porcine Gc were incubated with ITLN1 (10–100 ng/mL) for 24–72 h, and the viability/proliferation (alamarBlue/BrdU assays), cell cycle progression (flow cytometry) and the gene and protein expression of proliferation/apoptotic markers (PCNA, cyclins A1, B2, D1, E1, caspases-3, −9, BCL-2, BAX, FAS, FADD, XIAP) (real-time PCR, western blotting) were assessed. Next, the effect of ITLN1 on the phosphorylation of several kinases (AKT, AMPK, ERK1/2, STAT3, PKA) and the gene and protein expression of the insulin receptor (INSR) were studied (real-time PCR, western blotting). Then, using pharmacological inhibitors of ERK1/2 (PD98059, 5 μM), AKT (LY294002, 10 μM) and INSR (1 μM), treated alone or with ITLN1 (S961, 50 ng/mL), we analyzed its involvement in the effects of ITLN1 on Gc proliferation/apoptosis. We demonstrated that ITLN1 had a mitogenic effect on Gc by enhancing cell cycle progression and modulating the levels of PCNA, cyclins and apoptotic factors <em>via</em> ERK1/2, AKT, and INSR, suggesting that ITLN1 is a newly identified regulator in ovarian folliculogenesis, regardless of the fatness degree of pigs.</div></div>","PeriodicalId":12582,"journal":{"name":"General and comparative endocrinology","volume":"367 ","pages":"Article 114722"},"PeriodicalIF":2.1,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143843636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Using transcriptome analysis to investigate the induction of vitellogenesis in female Japanese eels (Anguilla japonica) 利用转录组分析研究日本鳗鲡(Anguilla japonica)卵黄形成的诱导机制
IF 2.1 3区 医学
General and comparative endocrinology Pub Date : 2025-04-12 DOI: 10.1016/j.ygcen.2025.114729
Chenpeng Zuo , Yonghang Zhang , Xuanhan Zhang , Jiaqi Liu , Likang Lyu , Teng Ma , Lingming Chen , Weimin Yu , Yun Li , Haishen Wen , Xin Qi
{"title":"Using transcriptome analysis to investigate the induction of vitellogenesis in female Japanese eels (Anguilla japonica)","authors":"Chenpeng Zuo ,&nbsp;Yonghang Zhang ,&nbsp;Xuanhan Zhang ,&nbsp;Jiaqi Liu ,&nbsp;Likang Lyu ,&nbsp;Teng Ma ,&nbsp;Lingming Chen ,&nbsp;Weimin Yu ,&nbsp;Yun Li ,&nbsp;Haishen Wen ,&nbsp;Xin Qi","doi":"10.1016/j.ygcen.2025.114729","DOIUrl":"10.1016/j.ygcen.2025.114729","url":null,"abstract":"<div><div>Oogenesis, encompassing folliculogenesis, development, and maturation, is a complex physiological process that is not solely regulated by gonadotropins but is also actively influenced by multiple growth factors produced by the oocyte and its surrounding follicular cells. The Japanese eel (<em>Anguilla japonica</em>) has a complex life history, resulting in many uncertainties regarding its growth, development, and reproduction. Under artificial culture conditions, oocyte development in the Japanese eel is arrested and can only progress to the vitellogenic stage through artificial induction. In the present study, we observed that, despite receiving the same hormone treatment as normally developing individuals, a small proportion of female eels exhibited oocytes arrested at the perinucleolar stage. Transcriptome analysis revealed that differentially expressed genes are involved in multiple reproductive-related physiological processes and functional pathways, such as tachykinin system, MAPK signaling pathway, steroid-related pathways, oocyte meiosis, Wnt signaling pathway and GnRH signaling pathway. The abnormal expression of the two follicle-stimulating hormone (FSH) subunit genes may be a key factor contributing to this phenomenon. This study reveals the underlying causes of ovarian developmental arrest in hormonally induced female Japanese eels from the perspective of the brain–pituitary–gonad (BPG) axis, providing a research foundation for the artificial reproduction of Japanese eels.</div></div>","PeriodicalId":12582,"journal":{"name":"General and comparative endocrinology","volume":"367 ","pages":"Article 114729"},"PeriodicalIF":2.1,"publicationDate":"2025-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143834673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Endocrine regulation of feeding in non-transgenic and transgenic fluorescent orange tiger barb (Puntigrus tetrazona) 非转基因和转基因荧光橙虎倒钩摄食的内分泌调节
IF 2.1 3区 医学
General and comparative endocrinology Pub Date : 2025-04-12 DOI: 10.1016/j.ygcen.2025.114730
Annika Lindstrom, Helene Volkoff
{"title":"Endocrine regulation of feeding in non-transgenic and transgenic fluorescent orange tiger barb (Puntigrus tetrazona)","authors":"Annika Lindstrom,&nbsp;Helene Volkoff","doi":"10.1016/j.ygcen.2025.114730","DOIUrl":"10.1016/j.ygcen.2025.114730","url":null,"abstract":"<div><div>Tiger barbs are popular tropical aquarium fish but despite their economic importance, nothing is known about their feeding physiology, in particular their endocrine regulation of feeding. The tiger barb has also been used to make genetically modified fluorescent fish but the influence of this genetic modification on their physiology is poorly understood. In this study, we submitted both non-transgenic (NT) and transgenic fluorescent orange (T) fish to 2 weeks of fasting or different temperatures (20, 25 and 30 °C) and assessed food intake and the expression of appetite regulators in brain, intestine and liver. Fasting had no effect on appetite regulators in the intestine, and decreased liver leptin expression in NT fish only. Fasting caused an overall increase and decrease in brain orexigenic and anorexigenic factors, respectively. The nature of peptides affected by this response differed between strains (MCH, ghrelin, POMCb in both NT and T, orexin in NT only, CRF and CCK in T only). In both T and NT fish, increasing temperatures increased food intake. Temperature affected the expression of most of the peptides examined, but the effects differed between the two fish strains. A shift from 25 to 20 °C increased hepatic leptin in NT and T, and intestine ghrelin in NT and had no effect on brain expression. A shift from 25 to 30 °C did not affect intestine or liver expressions, increased orexin, MCH and CRF brain expression in NT and T, and increased POMCb and CCKa expressions in T. Our study presents new information on the endocrine regulation of feeding in tiger barb, and provides insights on how transgenesis might affect feeding physiology of fish.</div></div>","PeriodicalId":12582,"journal":{"name":"General and comparative endocrinology","volume":"367 ","pages":"Article 114730"},"PeriodicalIF":2.1,"publicationDate":"2025-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143834674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Molecular identification and reproductive function of spexin in the big-belly seahorse (Hippocampus abdominalis) 大腹海马蛛丝蛋白的分子鉴定及生殖功能。
IF 2.1 3区 医学
General and comparative endocrinology Pub Date : 2025-04-06 DOI: 10.1016/j.ygcen.2025.114721
Limiao Zhao , Yuru Li , Jiajun Li , Weiqi Jin , Jun Chen , Bin Wang
{"title":"Molecular identification and reproductive function of spexin in the big-belly seahorse (Hippocampus abdominalis)","authors":"Limiao Zhao ,&nbsp;Yuru Li ,&nbsp;Jiajun Li ,&nbsp;Weiqi Jin ,&nbsp;Jun Chen ,&nbsp;Bin Wang","doi":"10.1016/j.ygcen.2025.114721","DOIUrl":"10.1016/j.ygcen.2025.114721","url":null,"abstract":"<div><div>Limited data are available regarding reproductive endocrinology of seahorse. Here, we reported the potential function of spexin (SPX1) in the reproduction of seahorse. SPX1, also known as neuropeptide Q (NPQ), is a novel neuropeptide that coevolved with galanin and kisspeptin. In the current study, the entire open reading frame (ORF) sequence of <em>spx1</em> of the big-belly seahorse (<em>Hippocampus abdominalis</em>) was cloned and characterized, which is 360 base pairs in length, encoding a 119-amino acid precursor peptide, with a 26-aa signaling peptide and a 14-aa C-terminal amidated mature peptide. Tissue distribution expression profiles of <em>spx1</em> transcripts were analyzed and revealed that <em>spx1</em> mRNA could be detected in a variety of tissues, with the highest abundance in the mixture tissues of brain and pituitary. The expression profile of <em>spx1</em> mRNA in the mixture of brain and pituitary during the first reproductive cycle of the big-belly seahorse was evaluated, which showed that the expression level of <em>spx1</em> mRNA was highest during the critical period of ovarian and testicular transition from stage II to stage III at 3.5-month old, and high in females with IV-stage, V-stage and VI-stage, as well as in males with the early-stage brood pouch, suggesting that SPX1 may play a crucial role at these stages of gonadal and brood pouch development. Intraperitoneal injection of SPX1 can suppress the mRNA expressions of <em>galr2b</em> and <em>fshβ</em> in female seahorses, however, it promoted the mRNA expressions of <em>spx1</em>, <em>gal</em>, <em>kiss2</em>, <em>gnrh2</em>, <em>kiss2r</em>, <em>galr1a</em>, <em>galr2b, gnrh3</em> and <em>gnihr</em> in males, besides, low doses of SPX1 enhanced <em>lhβ</em> mRNA expression, while high dose of SPX1 suppressed it. Intraperitoneal injection of SPX1 did not alter the mRNA levels of <em>spx1</em>, <em>gal</em>, <em>kiss2</em>, <em>galr1a</em>, <em>galr2a</em>, <em>gnihr</em> or plasma 17β-E2 in females, nor did it change the mRNA levels of <em>galr2a</em>, <em>gthα</em>, <em>fshβ</em> or plasma 11-KT in males. These results revealed that SPX1 may participate in regulating reproduction of the big-belly seahorse by controlling production of GnRH2, GnRH3, FSH and LH of HPG axis, as well as some key hypothalamic neuropeptides including Kiss and GAL. Above all, our results indicate the presence of a functional SPX1 system in the big-belly seahorse, as well as reveal its potential significance in the neuroendocrine regulation of reproduction in this species, which also lay a foundation for future research on optimizing fish reproductive performance through the regulation of SPX1.</div></div>","PeriodicalId":12582,"journal":{"name":"General and comparative endocrinology","volume":"367 ","pages":"Article 114721"},"PeriodicalIF":2.1,"publicationDate":"2025-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143810966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Global analysis of ligand-gated ion channel conservation across Platyhelminthes 配体门控离子通道保护的全局分析。
IF 2.1 3区 医学
General and comparative endocrinology Pub Date : 2025-04-01 DOI: 10.1016/j.ygcen.2025.114718
Víctor Hugo Caña-Bozada , Ahmed A.Z. Dawoud , Ivana Ramos-de la Cruz , Lizeth C. Flores-Méndez , Josué Barrera-Redondo , Jesús Briones-Mendoza , Luis A. Yañez-Guerra
{"title":"Global analysis of ligand-gated ion channel conservation across Platyhelminthes","authors":"Víctor Hugo Caña-Bozada ,&nbsp;Ahmed A.Z. Dawoud ,&nbsp;Ivana Ramos-de la Cruz ,&nbsp;Lizeth C. Flores-Méndez ,&nbsp;Josué Barrera-Redondo ,&nbsp;Jesús Briones-Mendoza ,&nbsp;Luis A. Yañez-Guerra","doi":"10.1016/j.ygcen.2025.114718","DOIUrl":"10.1016/j.ygcen.2025.114718","url":null,"abstract":"<div><div>Ligand-gated ion channels (LGICs) are critical for neurotransmission, mediating responses to neurotransmitters and hormones, and influencing diverse physiological processes. This study identifies and classifies LGICs across Platyhelminthes, with a particular focus on parasitic neodermatans, which impact human and animal health. Using bioinformatics tools, we analyzed LGICs from 41 neodermatan species and expanded our investigation to encompass vertebrates, other invertebrates, and non-bilaterians to trace LGIC evolutionary pathways across Metazoa. We identified 2,269 putative LGICs within neodermatan species, which we classified into the cys-loop, ASIC/Deg/ENaC, iGluR, and P2X families. Our phylogenetic and clustering analyses reveal lineage-specific patterns with distinct evolutionary trajectories for each LGIC family in neodermatans compared to free-living platyhelminths and other taxa. Notably, the ASIC/Deg/ENaC family displayed the greatest degree of neodermatan-specific divergence, while cys-loop and P2X families were more conserved across taxa. To provide insight into their potential physiological roles, we analyzed LGIC expression patterns in <em>Schistosoma mansoni</em>, revealing widespread expression across neuronal and muscle cell types. The distribution of acid-sensing ion channels (ASICs) in both neurons and muscles suggests a role in neuromuscular signalling, while the P2X receptor (Smp_333600) exhibited sex-specific expression, potentially indicating distinct functional roles in males and females. Additionally, several cys-loop acetylcholine and GABA receptors showed differential neuronal and muscle expression, highlighting their likely contributions to cholinergic and inhibitory neurotransmission. These findings underscore the relevance of LGICs in parasite physiology, particularly in neuromuscular and sensory processes, and suggest potential targets for antiparasitic interventions.</div></div>","PeriodicalId":12582,"journal":{"name":"General and comparative endocrinology","volume":"366 ","pages":"Article 114718"},"PeriodicalIF":2.1,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143742767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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