General and comparative endocrinology最新文献

{"title":"Multi-year profiles of T3 are positively correlated with corticosterone in male bowhead whale baleen","authors":"Justine M. Hudson ,&nbsp;Kathleen Hunt ,&nbsp;Marianne Marcoux ,&nbsp;C.Loren Buck ,&nbsp;Cory J.D. Matthews","doi":"10.1016/j.ygcen.2025.114800","DOIUrl":"10.1016/j.ygcen.2025.114800","url":null,"abstract":"<div><div>Thyroid hormones play an important role in the regulation of growth, development, metabolism, thermoregulation, and migration. Very little information exists on patterns of thyroid hormone concentrations in healthy mysticete whales, as many studies have focused on ill, entangled, or stranded whales, making it difficult to interpret thyroid hormone trends. In this study, we used a unique sample-set of bowhead whale baleen plates to explore the long-term interrelationships between triiodothyronine (T3), the most biologically active thyroid hormone, corticosterone, testosterone, and nitrogen isotope ratios (δ¹⁵N) (proxies for stress, reproduction, and diet, respectively) to investigate the role T3 may play in the physiology of healthy cetaceans. Baleen plates were collected between 1998 and 2011 from eight subsistence-harvested male bowhead whales across the Eastern Canadian Arctic. Each baleen plate generated 88–158 serial samples, representing ∼11–22 years of life for each individual whale. T3 concentrations ranged from 0.61 to 21.62 ng/g and varied seasonally in just two whales. Most whales showed no correlation between T3 and seasonal fluctuations in testosterone or δ¹⁵N, suggesting that variation in T3 is not driven by seasonal shifts in reproductive cycles, consumer trophic level, or migration. However, a strong positive correlation between T3 and corticosterone was observed in every whale, which we hypothesized was due to non-seasonal factors that simultaneously increase metabolic rate and physiological stress. The positive correlation between T3 with corticosterone suggests that in mysticete whales, some stressors may require increased energetic output.</div></div>","PeriodicalId":12582,"journal":{"name":"General and comparative endocrinology","volume":"373 ","pages":"Article 114800"},"PeriodicalIF":1.7,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144882683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Catecolaminergic systems and hypothalamic-pituitary-thyroid (HPT) axis: adrenaline, noradrenaline and dopamine treatments in vivo in lizard Podarcis siculus","authors":"Rosaria Sciarrillo ,&nbsp;Alessandra Falzarano ,&nbsp;Vito Gallicchio ,&nbsp;Assunta Lallo ,&nbsp;Francesca Carrella ,&nbsp;Aldo Mileo ,&nbsp;Anna Capaldo ,&nbsp;Maria De Falco","doi":"10.1016/j.ygcen.2025.114801","DOIUrl":"10.1016/j.ygcen.2025.114801","url":null,"abstract":"<div><div>The catecolaminergic system, which uses dopamine, noradrenaline and adrenaline as neurotransmitters, originates in the brainstem nuclei and spreads widely throughout the central nervous system (CNS). Although there are data indicating that interactions between the catecolaminergic systems of the nervous system and hypothalamic-pituitary-thyroid axis do indeed take place and may be of physiological and clinical relevance, such interactions are far from being clarified. In this paper, we studied the hypothalamic-pituitary-thyroid axis responses in lizards treated with adrenaline, noradrenaline and dopamine respectively. Adrenaline raised plasma TRH and TSH levels and induced a stimulatory effect on the thyroid gland activity with an increase of T₃ and T₄ levels. On the contrary, noradrenaline treatment induced a reduction in TRH secretion, accompanied by a normal circulating TSH level and increased plasma T₄ levels but with a reduction of circulating T₃ levels. Dopamine stimulated TRH secretion centrally but induced TSH deficiency followed by reduction of circulating T₃ and T₄ levels. Furthermore, the effects of the catecolaminergic system on the TRH-TSH-thyroid hormone axis has been also manifested through mechanisms in peripheral organs such as the liver. The dynamic interplay between TRH and TSH and the feedback effects on circulating thyroid hormones after treatment with adrenaline, noradrenaline and dopamine is a very sophisticated and complicated mechanism that responds via afferent inputs from neurons originating in the paraventricular nucleus and can influence the secretion of TRH and TSH-secreting hypophysiotropic neurons with consequent feedback on thyroid hormones.</div></div>","PeriodicalId":12582,"journal":{"name":"General and comparative endocrinology","volume":"373 ","pages":"Article 114801"},"PeriodicalIF":1.7,"publicationDate":"2025-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144872703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Neurosecretory protein GL stimulates feeding behavior and fat accumulation in Japanese quails (Coturnix japonica)","authors":"Masaki Kato,&nbsp;Eiko Iwakoshi-Ukena,&nbsp;Megumi Furumitsu,&nbsp;Yuki Narimatsu,&nbsp;Kazuyoshi Ukena","doi":"10.1016/j.ygcen.2025.114798","DOIUrl":"10.1016/j.ygcen.2025.114798","url":null,"abstract":"<div><div>Neurosecretory protein GL (NPGL) is a novel hypothalamic neuropeptide that promotes fat accumulation in rats, mice, and neonatal chicks. However, its role in Japanese quail (<em>Coturnix japonica</em>) remains unclear. In this study, we investigated the effects of chronic intracerebroventricular infusion of NPGL on body mass, food intake, and fat deposition in five-week-old male Japanese quails. A 13-day infusion of NPGL significantly increased body mass, food intake, and the masses of subcutaneous fat, abdominal fat, and liver. In contrast, water intake and the masses of the pancreas, testes, heart, and muscle remained unchanged. Blood concentrations of triglyceride, glucose, and non-esterified fatty acid were unaffected. Real-time qPCR analysis revealed a significant upregulation of <em>NPGM</em>, a paralogous gene of <em>NPGL</em>, in the hypothalamus. Additionally, the expression of stearoyl-CoA desaturase 1 (SCD1), a key enzyme involved in lipogenesis, showed an increasing trend in the liver. Although the fatty acid ratio used as the SCD1 activity index (palmitoleate/palmitate) did not change, the <em>de novo</em> lipogenesis index (palmitate/linoleate) tended to increase in both the liver and abdominal fat. These results suggest that NPGL promotes fat accumulation in Japanese quails, indicating its potential involvement in avian adiposity.</div></div>","PeriodicalId":12582,"journal":{"name":"General and comparative endocrinology","volume":"372 ","pages":"Article 114798"},"PeriodicalIF":1.7,"publicationDate":"2025-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144840774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regulation of transforming growth factor beta 1 in the ovary of ovoviviparous black rockfish (Sebastes schlegelii)","authors":"Xiao Jing,&nbsp;Likang Lyu,&nbsp;Chenpeng Zuo,&nbsp;Jianshuang Li,&nbsp;Xiaojie Wang,&nbsp;Jing Yang,&nbsp;Tianyu Jiang,&nbsp;Yun Li,&nbsp;Haishen Wen,&nbsp;Xin Qi","doi":"10.1016/j.ygcen.2025.114799","DOIUrl":"10.1016/j.ygcen.2025.114799","url":null,"abstract":"<div><div>Transforming growth factor beta 1 (TGF-β1), a multifunctional secreted polypeptide cytokine, has been reported to play crucial roles in pregnancy process of mammals, including immune tolerance and embryonic development. However, less is known in Pisces. Aimed to figure out the molecular mechanism underlying TGF-β1 functions, black rockfish (<em>Sebastes schlegelii</em>), an ovoviviparity teleost, which process the sperm storage and gestation periods, was employed as the research model. In the present study, we found that <em>tgfb1</em> among four <em>tgfb</em> isoforms was highly expressed and localized in ovary. The recombinant TGF-β1 (rTGF-β1) was mainly involved in immune response, signal transduction, angiogenesis and cell death by analyzing transcriptome of ovarian cells. The current results reveal the pivotal role of TGF-β1 in black rockfish ovary and provide novel insights into cytokines in the pregnancy of ovoviviparity teleost.</div></div>","PeriodicalId":12582,"journal":{"name":"General and comparative endocrinology","volume":"372 ","pages":"Article 114799"},"PeriodicalIF":1.7,"publicationDate":"2025-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144830268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Scale cortisol reflects the acute but not the chronic stress status in green sunfish (Lepomis cyanellus)","authors":"Abigayle M. McKinley,&nbsp;Carol Best,&nbsp;Nicholas J. Bernier","doi":"10.1016/j.ygcen.2025.114797","DOIUrl":"10.1016/j.ygcen.2025.114797","url":null,"abstract":"<div><div>Scale cortisol has the potential to be a useful biomarker of chronic stress in fish, but the dynamics of scale cortisol accumulation and release are not well understood. In this study, we assessed the dynamics of plasma and scale cortisol accumulation and clearance in green sunfish (<em>Lepomis cyanellus</em>) in response to acute and chronic stressors. While sunfish exposed to 21 days of unpredictable chronic stress (UCS) mounted an attenuated acute plasma cortisol response to the daily stressor on day 21 and had reduced liver and body conditions, their scale cortisol content was either unchanged or reduced. In contrast, exposure to an acute stressor increased scale cortisol content 30 min post-stressor in naïve fish but had no effect when preceded by the 21-day UCS protocol. We also examined the effects of an acute stressor on scale cortisol content in goldfish (<em>Carassius auratus</em>) to identify potential species differences in scale cortisol dynamics. Interestingly, although an acute stressor increased scale cortisol content 30 min post-stressor and declined 24 h later in both species, only sunfish scale cortisol levels returned to baseline by 24 h post-stressor. Together, our results indicate that scales in green sunfish can accumulate and clear cortisol rapidly following an acute stressor and do not reflect long-term accumulation of cortisol from the plasma during chronic stress. As such, we conclude that scale cortisol content is not a biomarker of chronic stress in green sunfish.</div></div>","PeriodicalId":12582,"journal":{"name":"General and comparative endocrinology","volume":"372 ","pages":"Article 114797"},"PeriodicalIF":1.7,"publicationDate":"2025-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144821184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Disorders of glucose metabolism and impaired energy metabolism in pfkma and pfkmb knockout zebrafish","authors":"Na Liu ,&nbsp;Mengjie Li ,&nbsp;Tianci Liu ,&nbsp;Jitu Wang ,&nbsp;Bo Zhang ,&nbsp;Man Zhang","doi":"10.1016/j.ygcen.2025.114787","DOIUrl":"10.1016/j.ygcen.2025.114787","url":null,"abstract":"<div><h3>Background</h3><div>In our previous study, Phosphofructokinase, muscle (PFKM), a key enzyme in glucose metabolism, was found to be decreased in the urinary exosomes of diabetic patients. The role of PFKM in glucose and energy metabolism is not fully understood. The study explores PFKM’s implications in these metabolic pathways through the lens of a zebrafish model.</div></div><div><h3>Methods</h3><div>We analyzed <em>pfkma</em> and <em>pfkmb</em> expressions in early zebrafish development using Daniocell and ZESTA databases and performed whole-mount <em>in situ</em> hybridization on embryos up to 4 days post-fertilization (dpf). The CRISPR/Cas9 system was utilized to generate <em>pfkma</em> and <em>pfkmb</em> knockout zebrafish, and the expression of various genes, including <em>β-actin, pfkma, pfkmb, peo1, mfn1, drp1, nd2, cytb, cox3,</em> and <em>atp6</em>, was measured by qRT-PCR in the embryos.</div></div><div><h3>Results</h3><div>Knockout mutants displayed elevated glucose levels across whole embryos. Additionally, at 5 dpf, <em>pfkma</em> and <em>pfkmb</em> double mutants showed pancreatic islet proliferation, ATP depletion, downregulation of the expression of the mitochondrial-related gene <em>peo1,</em> and impaired locomotor activity.</div></div><div><h3>Conclusions</h3><div>PFKM plays an important role in stabilizing glucose metabolism and has a certain impact on mitochondrial energy metabolism pathways in zebrafish model.</div></div>","PeriodicalId":12582,"journal":{"name":"General and comparative endocrinology","volume":"372 ","pages":"Article 114787"},"PeriodicalIF":1.7,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144717820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ontogeny of gonadotropin-inhibitory hormone cells and fibers in the brain and pituitary gland of the pejerrey fish, Odontesthes bonariensis","authors":"Ana M. Pahí-Rosero ,&nbsp;Gabriela C. López ,&nbsp;María R. Pérez ,&nbsp;Alejandro S. Mechaly ,&nbsp;Takayoshi Ubuka ,&nbsp;José A. Muñoz-Cueto ,&nbsp;Paula G. Vissio ,&nbsp;Gustavo M. Somoza","doi":"10.1016/j.ygcen.2025.114788","DOIUrl":"10.1016/j.ygcen.2025.114788","url":null,"abstract":"<div><div>The gonadotropin-inhibitory hormone is a neuropeptide belonging to the RF-amide family of peptides, first characterized in birds. This peptide can inhibit the synthesis and release of gonadotropins in both avians and mammals. Although gonadotropin-inhibitory hormone (Gnih) variants have also been detected in different fish species, knowledge of their physiological action is still inconclusive and controversial. In addition, there is very little information on the neuronal development of Gnih cells in early stages. In this context, the objective of the present study is to characterize Gnih-containing neurons and fibers in the brain and pituitary gland of adult fish, and during early development of the pejerrey (<em>Odontesthes bonariensis</em>), and the possible relationship with the sex differentiation process.</div><div>The Gnih neurons and fibers were determined by immunohistochemistry by using different antisera in the adult pejerrey brain. Gnih-immunoreactive (Gnih-ir) neurons were observed in the olfactory bulbs, the terminal nerve ganglion, the lateral nuclei of the ventral telencephalon, the posterior periventricular nucleus, the dorsal tegmentum, and some isolated neuronal bodies were detected in the secondary gustatory nucleus. Very few Gnih-ir fibers were detected innervating the pituitary gland.</div><div>The Gnih neuronal and fiber distribution was also studied from hatching to week 10, covering the sex differentiation period until the gonadal sex was observed. Gnih-immunoreactive neuronal bodies were identified from hatching in the terminal nerve ganglion and the diencephalic posterior periventricular nucleus. Gnih-ir fibers were observed in many regions, including the retina, and a profuse innervation was observed in the pituitary. From the first week post-hatching, Gnih-ir neuronal bodies were identified in the dorsal mesencephalic tegmentum and the lateral nucleus of the ventral telencephalon. In addition, Gnih-ir cells were detected in the pituitary. These Gnih-ir cells were consistently detected from 3 to 7 weeks after hatching, coinciding with onset of gonadal differentiation. At week 10 and in the adult, only a few Gnih-ir fibers were observed in the pituitary. Although the precise function of these pituitary Gnih-ir cells is unknown, the relationship between the appearance of these cells and the process of sex differentiation suggests that Gnih may influence this process.</div></div>","PeriodicalId":12582,"journal":{"name":"General and comparative endocrinology","volume":"372 ","pages":"Article 114788"},"PeriodicalIF":2.1,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144665536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vitro effects of polycyclic aromatic hydrocarbons on sex steroid production by Arabian sea bream Acanthopagrus arabicus ovarian cells","authors":"Fereshteh Aysham ,&nbsp;Negin Salamat ,&nbsp;Alireza Safahieh ,&nbsp;Arash Larki ,&nbsp;Asma Mohammadi","doi":"10.1016/j.ygcen.2025.114778","DOIUrl":"10.1016/j.ygcen.2025.114778","url":null,"abstract":"<div><div>Polycyclic aromatic hydrocarbons (PAHs), considered endocrine disruptors, affect the animals reproduction by interfering with the production of sex steroids. In the present study, the in vitro effects of selected PAHs (naphthalene, phenanthrene and BaP) on steroid production of cultivated ovarian cells from Arabian sea bream (<em>Acanthopagrus arabicus</em>) was assessed. Ovarian cells were cultured in Leibovitz L-15 (L-15) medium containing naphthalene, phenanthrene and benzo(a)pyrene with or without androstenedione (AD) as precursors and17b-estradiol (E2), progesterone (P4) and testosterone (T) were then measured. The effects of selected PAHs on the steroidogenic enzyme systems cytochrome P450 17, 20-lyase (P450-17,20l) and cytochrome P450 aromatase (P450-arom) were also compared with the ketoconazole (KCZ) action as inhibitor of cytochrome P450 steroidogenic enzymes (cytochrome P450 17, 20-lyase (P450-17,20l)) and cytochrome P450 aromatase (P450-arom). Adding exogenous androstenedione as a steroid precursor significantly increased the production of all three steroid hormones by cultured ovarian cells. On the other hand, the addition of ketoconazole significantly reduced the production of E2 and P from ovarian cells, while it had no effect on the production of T. This result showed that ketoconazole only affects the cytochrome P450 steroidogenic enzymes and has no effect on the 17-beta-hydroxysteroid dehydrogenase (17-βHSD) enzyme that convert androstenedione to testosterone. On the other hand, a significant decrease in the production of all three steroids (17β-estradiol, progesterone, and testosterone) from ovarian cells exposed to the studied pollutants (naphthalene, phenanthrene, and benzo(a)pyrene) even in the presence of androstenedione indicated that PAHs inhibit all three steroidogenic enzyme systems including P450-17,20l, P450-arom, and 17b-HSD. In conclusion, PAHs are potent inhibitors of the steroidogenic enzyme system including P450-17,20l, P450-arom, and 17b-HSD, and therefore, they can disturb the reproduction of fish living in contaminated areas due to impairment of steroid biosynthesis.</div></div>","PeriodicalId":12582,"journal":{"name":"General and comparative endocrinology","volume":"371 ","pages":"Article 114778"},"PeriodicalIF":2.1,"publicationDate":"2025-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144579745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of a crustacean hyperglycemic hormone of the horsehair crab Erimacrus isenbeckii","authors":"Kenji Toyota ,&nbsp;Asami Kajimoto ,&nbsp;Yushi Ando ,&nbsp;Ken Takeuchi ,&nbsp;Tsuyoshi Ohira","doi":"10.1016/j.ygcen.2025.114777","DOIUrl":"10.1016/j.ygcen.2025.114777","url":null,"abstract":"<div><div>The horsehair crab <em>Erimacrus isenbeckii</em> is widely distributed from Alaska and the Bering Sea through Southern Sakhalin, and in Japan from the coastal areas of Hokkaido to the Sea of Japan. In the Hokkaido area, although strict resource management has been promoted by setting an allowable catch limit, the catch amount has remained at a low level. While knowledge on larval rearing methods is accumulating in relation to seedling production techniques, information on adult growth and molting is limited, due to a deep-sea species that requires a long period for growth. In decapod crustaceans, the sinus-gland/X-organ complex in the eyestalk ganglion synthesizes and secrets various neuropeptides such as crustacean hyperglycemic hormone (CHH) to regulate the homeostasis of haemolymph glucose levels. In this study, combined isolation of sinus gland peptides by reverse-phase high-performance liquid chromatography (RP-HPLC) and amino acid sequencing, and transcriptome analyses using male and female eyestalk ganglion has been successfully identified EiCHHa. In adult male <em>E</em>. <em>isenbeckii</em>, no decrease in haemolymph glucose levels was observed following bilateral eyestalk ablation, indicating that this treatment does not affect glucose homeostasis in this species. Therefore, the <em>in vivo</em> assays of EiCHHa using the blue swimming crab <em>Portunus pelagicus</em> revealed EiCHHa has a hyperglycemic effect, as well as CHHs in other decapod crustaceans. On the other hand, EiCHHa-injected red swamp crayfish <em>Procambarus clarkii</em> with bilateral eyestalk ablation showed no significant increase in hemolymph glucose levels. Additionally, we successfully demonstrated the sexual differences in the transcriptomic profiles between males and females. Especially, two sinus gland-derived neuropeptides (EiCHHb and a crustacean female sex hormone (EiCFSH)) were isolated as female-biased transcripts, suggesting that both hormones may have female-specific roles such as the development of female characteristics and reproduction.</div></div>","PeriodicalId":12582,"journal":{"name":"General and comparative endocrinology","volume":"371 ","pages":"Article 114777"},"PeriodicalIF":2.1,"publicationDate":"2025-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144571801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vitro effects of 11-deoxycorticosterone on hepatocytes and gill epithelial cells of rainbow trout (Oncorhynchus mykiss)","authors":"Rodrigo Zuloaga ,&nbsp;Alfredo Molina ,&nbsp;Juan Antonio Valdés","doi":"10.1016/j.ygcen.2025.114776","DOIUrl":"10.1016/j.ygcen.2025.114776","url":null,"abstract":"<div><div>The 11-deoxycorticosterone (DOC) corticosteroid has been recently described as having a potential role in fish, complementing cortisol action through distinct physiological effects. Although systemic effects of 11-deoxycorticosterone (DOC) have been reported, the specific mechanisms mediated through glucocorticoid (GR) and mineralocorticoid (MR) receptors remain poorly understood. Therefore, we evaluated the DOC effects through both receptors in rainbow trout hepatoma-derived (RTH-149) and gill epithelial (RTgill-W1) cell lines. Cultures were pretreated with GR (Mifepristone) or MR (Eplerenone) antagonists for 1 h (50 nM) and then with DOC (10 nM) or vehicle (DMSO-PBS1X) as control for 3 h (n = 3). First, to determine a DOC-induced response via GR or MR, we detected a decrease in transcriptional levels of <em>mr</em> and these results were recovered to basal levels by MR antagonist in both cell lines. Then, we evaluated different metabolites and solutes associated with carbohydrate metabolism in RTH-149 and osmoregulation in RTgill-W1, detecting that DOC through both GR and MR differentially modulates lactate, glycogen, calcium, and chloride levels. We also identified that DOC mainly by MR differentially regulates gene expression of glucose/glycogen metabolism in RTH-149, ionic cotransporters, and tight junction proteins in RTgill-W1. Subsequently, we determined that DOC significantly decreases glucose uptake in RTH-149 and apparent permeability in RTgill-W1, reversed by MR antagonist. However, DOC does not affect transepithelial resistance in RTgill-W1. This study provides the first evidence that DOC, primarily via MR, plays a role in regulating carbohydrate metabolism in fish hepatocytes and osmoregulation in gill epithelial cells.</div></div>","PeriodicalId":12582,"journal":{"name":"General and comparative endocrinology","volume":"371 ","pages":"Article 114776"},"PeriodicalIF":2.1,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144524004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}