General and comparative endocrinology最新文献

{"title":"Asprosin modulates female reproductive functions in teleosts: An in vitro study in Channa punctata","authors":"Priyanka Sathoria ,&nbsp;Anju Shrivastava ,&nbsp;Umesh Rai ,&nbsp;Brototi Roy","doi":"10.1016/j.ygcen.2025.114770","DOIUrl":"10.1016/j.ygcen.2025.114770","url":null,"abstract":"<div><div>Asprosin regulates various aspects of physiology in mammals including reproduction. It is reported to profoundly affect both male and female reproductive functions including gametogenesis and steroidogenesis. Asprosin is the cleaved product of a profibrillin protein encoded by the <em>fbn1</em> gene. For the first time in non-mammalian vertebrates, our group demonstrated the ubiquitous expression of <em>fbn1</em> and characterized asprosin protein <em>in silico</em> in teleost <em>Channa punctata</em> commonly known as spotted snakehead (ss). Based on the prominent expression of the <em>fbn1</em> gene and the reproductive phase-dependent temporal expression of <em>fbn1</em> in the ovary of <em>C. punctata</em>, we hypothesized the regulatory role of asprosin in female reproduction similar to that reported in mammals. <em>In vitro</em> studies confirmed the effect of asprosin on the oogenesis and steroidogenesis in <em>C. punctata</em>. Asprosin significantly enhanced the expression of genes crucial for oogenesis such as <em>pcna</em> and <em>gdf9</em>. It also increased the transcription of gonadotropin receptors and sex steroid receptor genes. In addition to this, asprosin accentuated the expression of steroidogenic markers such as <em>star</em> and <em>cyp17a1</em> along with 17α, 20β dihydroxy-progesterone levels. We also measured the levels of the second messenger cAMP in ovaries exposed to asprosin to explore the probability of GPCRs as asprosin receptors. However, asprosin could not alter the cAMP levels indicating that, in the ovary of teleosts, receptors other than GPCRs might be involved in transducing asprosin action. Thus, the present study in elucidates the important role of asprosin in modulating the ovarian functions in <em>C. punctata</em>.</div></div>","PeriodicalId":12582,"journal":{"name":"General and comparative endocrinology","volume":"370 ","pages":"Article 114770"},"PeriodicalIF":2.1,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144281111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Relationships between ambient temperature and diurnal variation in physiological responses to acute handling and restraint stress in the house sparrow, Passer domesticus","authors":"Pierre Deviche ,&nbsp;Sarah E. Polekoff ,&nbsp;Ondi L. Crino ,&nbsp;Justin R. Eastwood ,&nbsp;Simon C. Griffith ,&nbsp;Katherine L. Buchanan","doi":"10.1016/j.ygcen.2025.114767","DOIUrl":"10.1016/j.ygcen.2025.114767","url":null,"abstract":"<div><div>Climate change is altering the frequency and intensity of environmental extremes, and the diurnal rates of environmental change. The daily reaction norms of wild animals show spatial and temporal plasticity to allow appropriate physiological responses to predictable environmental challenges, but these responses have rarely been quantified in wild birds. We addressed this by determining whether physiological stress indices (corticosterone [CORT], glucose [GLU], and circulating heat shock proteins [Hsp]) vary with ambient temperature (T_a) or time of day across a 6⁰ latitudinal gradient (32⁰–38⁰ S). We sampled house sparrows, <em>Passer domesticus</em>, at three locations with varying daily rates of T_a increases in 2015 and in 2019–2020 to determine whether physiological responses to capture and handling stress change diurnally. As predicted, plasma CORT and GLU increased during stress. Baseline CORT and GLU levels did not change during the day, but the amplitude of plasma CORT increase (delta CORT) in response to capture decreased as T_a increased, as was the case for GLU, in birds with initially high baseline CORT. Neither baseline nor stress-related plasma CORT or GLU differed consistently across sampling locations. Heat-shock cognate 70 (Hsc70) and heat-shock protein 90α (Hsp90) showed clear temporal dynamics across the day. Thus, the reaction norms of heat shock proteins are temporally plastic during the day and in response to daily T_a changes, as are the reaction norms of CORT and GLU in response to acute stress. However, the study provides little evidence for such plasticity in reaction norms as a function of average local thermal conditions.</div></div>","PeriodicalId":12582,"journal":{"name":"General and comparative endocrinology","volume":"370 ","pages":"Article 114767"},"PeriodicalIF":2.1,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144281110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Amino acid residues critical to cAMP signaling by V2a-type vasopressin/vasotocin receptor: an evolutionary perspective","authors":"Yoko Yamaguchi","doi":"10.1016/j.ygcen.2025.114758","DOIUrl":"10.1016/j.ygcen.2025.114758","url":null,"abstract":"<div><div>Vasopressin (VP) and its non-mammalian homolog vasotocin (VT) are secreted from the posterior pituitary to control various physiological phenomena via G protein-coupled receptors. The six known subtypes of VP/VT receptor family (V1a/bR and V2a/b/c/dR) originate from a single ancestral molecule, that most likely coupled with Gα_q and used Ca²⁺ for signaling. However, among the current VP/VT receptors, V2aR predominantly couples with Gα_s and increases intracellular cAMP levels. This receptor is also known to mediate the potent antidiuretic effect of VP/VT, and therefore is clinically important. Although the structure-function relationship of V2R has been of great interest to researchers, an evolutionary perspective was missing; how did the ancestral V2aR switched its G protein selectivity? To address this question, a comparative study between V2aR and its Ca²⁺-signaling cognates (V2b/c/dR) is needed. The aim of the present study was to identify residues/motifs that are crucial to cAMP signaling by V2aR; chimeric receptors were engineered using the functional V2aR and V2bR of medaka. <em>In vitro</em> assays demonstrated that Gα_s-coupling ability of the receptors can be altered by swapping a single residue in the second intracellular loop (ICL2), without affecting Gα_q-coupling ability. The three-dimensional models predicted <em>in silico</em> suggested that the interaction between ICL2 and Gα_s-αN chain and Gα_s-β2/β3 loop contributes to the stabilization of the signaling complex, enhancing the receptor’s capacity to stimulate cAMP pathway upon ligand binding. These findings provide new insights into the molecular and functional evolution of V2aR, as well as into the mechanisms of G protein selectivity of GPCR.</div></div>","PeriodicalId":12582,"journal":{"name":"General and comparative endocrinology","volume":"370 ","pages":"Article 114758"},"PeriodicalIF":2.1,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144230937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cholecystokinin and gastrin-releasing peptide differentially inhibit appetite of rainbow trout","authors":"Antti Forsman ,&nbsp;Elisabeth Jönsson ,&nbsp;Björn Thrandur Björnsson ,&nbsp;Katja Anttila ,&nbsp;Kari Ruohonen","doi":"10.1016/j.ygcen.2025.114757","DOIUrl":"10.1016/j.ygcen.2025.114757","url":null,"abstract":"<div><div>The appetite in fish is a multifaceted phenomenon that comprises specialized interactions between brain and peripheral signals, and as a result, appetite is either stimulated or inhibited. Cholecystokinin (Cck) and gastrin-releasing peptide (Grp) are two postprandially released gastrointestinal peptide hormones that affect feed intake in fish. As the stimulatory or inhibitory effects of hormones can vary in duration and strength, making the nature of hormone effects dynamic, we modelled the dynamics of Cck and Grp using a direct, non-stressful approach. Fish were hormonally treated through an intraperitoneal cannula and feed intake was monitored for 12 h post-injection using a self-feeder system. Cck and Grp decreased feed intake in a dose-dependent manner, hormone-specific both in terms of magnitude and duration. Cck had an immediate inhibitory effect on feed intake, which lasted two–three hours, whereas the immediate inhibitory effect of Grp lasted for the entire 12-hour observation period. The data suggest that Cck acts as a short-term satiety signal in rainbow trout, while Grp acts as a longer-term appetite suppressor.</div></div>","PeriodicalId":12582,"journal":{"name":"General and comparative endocrinology","volume":"370 ","pages":"Article 114757"},"PeriodicalIF":2.1,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144225101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Puberty regulation in chub mackerel Scomber japonicus, an important aquaculture fish species, via reproductive endocrine mechanism","authors":"Hirofumi Ohga","doi":"10.1016/j.ygcen.2025.114735","DOIUrl":"10.1016/j.ygcen.2025.114735","url":null,"abstract":"<div><div>The vertebrate reproductive system is controlled by the brain–pituitary–gonadal reproductive endocrine axis (BPG axis). Gonadotropin-releasing hormone (GnRH) secreted from the hypothalamus regulates the secretion of two gonadotropic hormones (GTHs), follicle-stimulating hormone (FSH) and luteinizing hormone (LH), in the pituitary gland. Steroid hormones, such as androgens and estrogens, secreted in response to GTH stimulation, regulate the development of male and female gametes. Recently, various neuropeptides have been identified in mammals as factors that regulate the BPG axis from the upstream, demonstrating their importance. The author’s main research theme is “Understanding the mechanism underlying puberty control in aquaculturally important species.” They have been actively investigating the regulatory mechanism of the BPG axis in the puberty of chub mackerel <em>Scomber japonicus</em>, an important fishery species. With this species, researchers can systematically collect target organs, cells, and other organs from individuals at each developmental stage, from fertilized eggs to adult fish after spawning. A highly reproducible rearing experimental system has been established, enabling verification of the knowledge gained through feedback to reared individuals. Furthermore, it has recently become possible to quickly and efficiently produce gene knockout lines using genome editing. This article introduces the mechanism of puberty control in chub mackerel by fully leveraging this experimental platform. Focus will be directed to the functions of kisspeptin, which triggers puberty by regulating GnRH secretion in mammals, and leptin, which transmits nutritional information to the reproductive axis.</div></div>","PeriodicalId":12582,"journal":{"name":"General and comparative endocrinology","volume":"369 ","pages":"Article 114735"},"PeriodicalIF":2.1,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144015621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metamorphosis-associated immune system maturation in Senegalese sole","authors":"Sandra C. Silva ,&nbsp;Teresa M. Correia ,&nbsp;Manuel Manchado ,&nbsp;Deborah M. Power","doi":"10.1016/j.ygcen.2025.114755","DOIUrl":"10.1016/j.ygcen.2025.114755","url":null,"abstract":"<div><div>The thyroid hormones (THs) are proposed as putative regulators of immune system maturation in developing teleost fish. To gain insight into this process the Senegalese sole (<em>Solea senegalensis</em>) that has a well-characterized TH-driven metamorphosis was used. Differential gene expression analysis was performed across developmental stages (n = 3 per stage): pre-metamorphosis, onset of metamorphosis, metamorphosis, early climax, climax, and post-metamorphic juveniles. Metamorphosis is a massive gene-oriented developmental process, involving the differential expression of 8145 genes. Clustering analysis was used to identify immune-related genes with similar expression patterns to hypothalamus-pituitary-thyroid (HPT) axis-related genes. TH-regulated candidate immune genes were identified (133) and analysis of their promoter region revealed 84 contained putative TH receptor (TR) binding sites (TREs). Two consensus TRE sequences were identified in the candidate genes, 5′-<em>n</em>tg<em>n</em>G<em>n</em>tCaca<em>n</em> (exclusive to TRα), and 5′-<em>nnn</em>tGgtCa<em>nnn</em> (common to both TRs). TRα-exclusive TREs were less common than those that bound interchangeably TRα and TRβ. In the promoter region, TRα-exclusive TREs were always accompanied by the pan-TRE consensus sequence, never occurring independently.</div></div>","PeriodicalId":12582,"journal":{"name":"General and comparative endocrinology","volume":"369 ","pages":"Article 114755"},"PeriodicalIF":2.1,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144205599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sex steroid profiles align with phenotype in sex-reversed female lizards","authors":"Naomi E. Laven ,&nbsp;Phillip R. Pearson ,&nbsp;Kristoffer H. Wild ,&nbsp;Daniel W.A. Noble ,&nbsp;Ondi L. Crino","doi":"10.1016/j.ygcen.2025.114754","DOIUrl":"10.1016/j.ygcen.2025.114754","url":null,"abstract":"<div><div>Vertebrate sex determination is remarkably diverse. In species with genetic sex determination, reproductive physiology and sex-specific behaviour are influenced by sex chromosomes and sex steroid hormones. However, some species experience sex reversal where the influence of sex chromosomes on sexual development is modulated by mutations or environmental conditions, leading to animals that phenotypically mismatch with their genotype. In these species, sex-reversed (‘discordant’) individuals can theoretically exhibit traits that are similar to either their phenotypic or genotypic sex. In the central bearded dragon (<em>Pogona vitticeps</em>), high incubation temperatures produce sex-reversed females with male-type sex chromosomes (ZZf). ZZf display a mix of male-like and intermediate traits compared to males (ZZm) and concordant females (ZWf). Sex steroid profiles could drive these differences but are yet to be examined in <em>P. vitticeps</em>. We measured testosterone (T) and estradiol (E2) in ZWf, ZZf, and ZZm dragons at three timepoints across a breeding season. As sex steroids can potentially affect offspring if maternal hormones are transmitted to eggs, we also compared T and E2 levels in egg yolks from ZZf and ZWf females. Sex-reversed ZZf had lower T levels than males and similar T and E2 levels to ZWf across the breeding season. ZWf and ZZf laid eggs with similar levels of T and E2. We found a negative association between maternal and yolk E2 levels, but no association between maternal and yolk T. Our results show that sex steroid profiles in sex-reversed ZZ female <em>P. vitticeps</em> reflect phenotypic, not genotypic sex, suggesting circulating sex steroids do not drive trait differences between discordant and concordant females.</div></div>","PeriodicalId":12582,"journal":{"name":"General and comparative endocrinology","volume":"369 ","pages":"Article 114754"},"PeriodicalIF":2.1,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144186858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of the nuclear localization sequence and C-terminus of Parathyroid hormone-related protein on the growth Hormone-IGF-1 axis","authors":"Waleed J. Hashmi ,&nbsp;Megan M. Turner ,&nbsp;Ibiagbani M. Max-Harry ,&nbsp;Nathan K. Hoggard ,&nbsp;Alex H. Fischbach ,&nbsp;Shiyu Yuan ,&nbsp;Emily Davis ,&nbsp;Noriko Kantake ,&nbsp;John J. Kopchick ,&nbsp;Blake E. Hildreth III ,&nbsp;Ramiro E. Toribio ,&nbsp;Thomas J. Rosol","doi":"10.1016/j.ygcen.2025.114756","DOIUrl":"10.1016/j.ygcen.2025.114756","url":null,"abstract":"<div><div>Parathyroid hormone–related protein (PTHrP) is a polyhormone consisting of an N-terminus, a mid-region, a nuclear localization sequence (NLS), and a C-terminus. The NLS and C-terminus of PTHrP regulate endochondral bone formation, craniofacial development, hematopoiesis, and survival. Our laboratory has developed <em>Pthrp</em> Δ/Δ mice lacking the NLS and C-terminus of PTHrP, which exhibit severe growth delay and early mortality within the first week of life. This study investigates the growth hormone (GH)-IGF-1 axis in <em>Pthrp</em> Δ/Δ mice. PTHrP is expressed by various endocrine cells, including pituitary endocrine epithelial cells of the adenohypophysis. Histopathological, biochemical, ultrastructural, and gene expression analyses were performed on the pituitary from <em>Pthrp</em> Δ/Δ and age-matched control mice. Pituitary glands from <em>Pthrp</em> Δ/Δ mice had normal cellularity; however, the pituitary somatotrophs had increased <em>Gh</em> mRNA expression with a decrease in the number and size of cytoplasmic secretory granules containing GH. Plasma GH concentrations were either normal or increased, and plasma ACTH concentrations were increased. Western blot analysis of the pituitary glands revealed reduced GH in <em>Pthrp</em> Δ/Δ mice. The plasma concentrations of IGF-1 and liver <em>Igf1</em> mRNA expression and glycogen content were decreased in <em>Pthrp</em> Δ/Δ mice. Western blot analysis of liver from <em>Pthrp</em> Δ/Δ mice showed a significant reduction in both total and phosphorylated Janus Kinase 2 (JAK2) proteins and total and tyrosine-phosphorylated STAT5b proteins compared to controls. In conclusion, the lack of the NLS and C-terminus of PTHrP disrupted the normal GH-IGF-1 axis, leading to impaired IGF-1 production by the liver.</div></div>","PeriodicalId":12582,"journal":{"name":"General and comparative endocrinology","volume":"369 ","pages":"Article 114756"},"PeriodicalIF":2.1,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144184783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nest building and circulating testosterone dynamics in male zebra finches, Taeniopygia guttata","authors":"Marie Hébert ,&nbsp;Eira Ihalainen ,&nbsp;Sophie C. Edwards ,&nbsp;Simone L. Meddle ,&nbsp;Susan D. Healy","doi":"10.1016/j.ygcen.2025.114753","DOIUrl":"10.1016/j.ygcen.2025.114753","url":null,"abstract":"<div><div>Despite its fundamental importance for avian reproduction, the hormonal mechanisms supporting nest building remain poorly understood. While nest building by both male and female builders requires circulating sex steroids, it is not clear whether these steroids are required throughout building. We, therefore, sought to determine circulating testosterone dynamics in male zebra finches during early breeding, specifically focusing on the nest building period. We collected plasma samples from two cohorts of birds at three of the following four timepoints: before (baseline), following pairing, during, and after nest building. To dissociate the end of nest building from incubation onset we daily removed any eggs laid and terminated nest building once we had sampled males during nest building. Male circulating testosterone levels remained stable following pairing with a female and throughout nest building. However, testosterone titers dropped once nest building had stopped. We also found evidence to suggest that, following pairing with a female, circulating testosterone titers can predict a male’s future readiness to build. Together, our data suggest that circulating testosterone is important throughout nest building, not decreasing until the end of nest building activities, perhaps through a ‘self-feedback’ mechanism.</div></div>","PeriodicalId":12582,"journal":{"name":"General and comparative endocrinology","volume":"369 ","pages":"Article 114753"},"PeriodicalIF":2.1,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144142279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of dietary quality on melatonin in gut, serum and ovary in mediating growth, digestive and ovarian physiology in juvenile carp (Catla catla)","authors":"Farha Yasmin,&nbsp;Sona Sutradhar,&nbsp;Arun Roy,&nbsp;Sourav Mukherjee","doi":"10.1016/j.ygcen.2025.114752","DOIUrl":"10.1016/j.ygcen.2025.114752","url":null,"abstract":"<div><div>The dietary protein and tryptophan of fish feed have vital roles in the growth performance, stress management, and digestive physiology of fish. However, in this context, the functions of gut melatonin, which depend on the availability of food, the timing of food supply, the frequency of feeds/day, the quality of food, and the growth stages of carp, still need to be clarified. Hence, the present study aimed to investigate the impact of dietary quality on specific growth rate (SGR), residual gut content (RGC), feeding intensity (FI), gut melatonin, gut oxidative stress and its major antioxidants, vital digestive enzymes in juvenile carp, <em>Catla catla</em> (mean body weight: 274 ± 16.63 g). Besides, serum melatonin and ovarian melatonin, gonado-somatic index (GSI), and different growth stages of oocytes were also assessed before the estimation of oxidative stress and major antioxidant enzymes in the ovary. The fish were fed four diets viz. (i) a standard diet (SD/control) (with 34.99 % protein, 14.56 % carbohydrate, 9.84 % oil, and 0.36 % L-tryptophan) (ii) one protein (PRD with 41.02 % protein and 0.46 % L-tryptophan), (iii) two L-tryptophan (TrpRD1 with 0.96 %, and TrpRD2 with 1.36 % tryptophan), -rich diets for 30 days. The SGR was higher only in PRD, but feeding was reduced in PRD and TrpRDs compared to SD-fed carp. However, the PRD and TrpRDs stimulated gut melatonin, digestive enzymes and all the studied antioxidants with reduced oxidative stress by lowering malondialdehyde (MDA). Moreover, a parallel increase in serum melatonin and ovarian melatonin with gut melatonin has been noticed in both the PRD and TrpRDs. All these experimental diets showed pro-gonadal effects by increasing stage-I oocytes and by reducing oxidative stress in the developing ovary in juvenile carp, compared to SD-fed carp. This opens avenues for future research on the role of feed-induced gut melatonin in fish nutrition and reproduction.</div></div>","PeriodicalId":12582,"journal":{"name":"General and comparative endocrinology","volume":"369 ","pages":"Article 114752"},"PeriodicalIF":2.1,"publicationDate":"2025-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144101602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}