Genetics and Molecular Biology最新文献_第8页

Soybean genetic engineering in Brazil and South America - A review. 巴西和南美大豆基因工程研究综述。

IF 1.7 4区生物学

Genetics and Molecular Biology Pub Date : 2026-02-13 eCollection Date: 2026-01-01 DOI: 10.1590/1678-4685-GMB-2025-0007

Renata Fuganti-Pagliarini, Mayla Daiane Correa Molinari, João Matheus Kafer, Alexandre Lima Nepomuceno, Liliane Marcia Mertz Henning, Sérgio Hermínio Brommonschenkel, Larissa Goulart Zanardo, Maria Helena Bodanese-Zanettini, Elibio Rech

{"title":"Soybean genetic engineering in Brazil and South America - A review.","authors":"Renata Fuganti-Pagliarini, Mayla Daiane Correa Molinari, João Matheus Kafer, Alexandre Lima Nepomuceno, Liliane Marcia Mertz Henning, Sérgio Hermínio Brommonschenkel, Larissa Goulart Zanardo, Maria Helena Bodanese-Zanettini, Elibio Rech","doi":"10.1590/1678-4685-GMB-2025-0007","DOIUrl":"10.1590/1678-4685-GMB-2025-0007","url":null,"abstract":"Soybean like other commodities brings important income to producer countries. In Brazil, the picture is not different. Thus, due to its economic significance and considering all field challenges, that impose abiotic and biotic constraints to improve yield, research efforts have focused on solving problems by applying biotechnological tools. In this review, a description of genetic engineering being developed in Brazil for soybean crop is addressed, including research in tolerance to abiotic conditions such as drought; to biotic factors such as insects, nematodes and fungal among other categories; as well as the use of soybean as bio-factories. All commercially available soybean events in Brazil and South American countries, for insect resistance and herbicide tolerance are also discussed. An overview of the Brazilian regulatory framework and South American countries for Biotech products is presented as well as the future perspectives for soybean genetic engineering in Brazil. In addition, a list of papers, from 2008 to the present, showing the state of the art of genetic engineering of soybean and by-products in Brazil is made available. The same search on the literature for South American countries did not return papers published on the genetic engineering of soybean and by-products. Although South American countries did not develop science on GM soybean, a search on GM varieties being sowed indicated that these countries adopted GM technology, due to all the advantages that these lines offer.","PeriodicalId":12557,"journal":{"name":"Genetics and Molecular Biology","volume":"49 1","pages":"e20250007"},"PeriodicalIF":1.7,"publicationDate":"2026-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12965416/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147369191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Global patterns, biases, and advances in phylogeographic and genetic structure studies in Drosophilidae (Insecta: Diptera). 果蝇科（昆虫亚目：双翅目）系统地理学和遗传结构研究的全球格局、偏差和进展。

IF 1.3 4区生物学

Genetics and Molecular Biology Pub Date : 2026-02-09 eCollection Date: 2026-01-01 DOI: 10.1590/1678-4685-GMB-2025-0246

Henrique R M Antoniolli, Maríndia Deprá, Lizandra Jaqueline Robe

{"title":"Global patterns, biases, and advances in phylogeographic and genetic structure studies in Drosophilidae (Insecta: Diptera).","authors":"Henrique R M Antoniolli, Maríndia Deprá, Lizandra Jaqueline Robe","doi":"10.1590/1678-4685-GMB-2025-0246","DOIUrl":"10.1590/1678-4685-GMB-2025-0246","url":null,"abstract":"Phylogeographic and genetic structure studies involving Drosophilids have clarified numerous processes that have shaped the evolution of biodiversity over time. In this review, we aim to (i) assess the main biases, gaps, and advances in the scientific literature on this topic; (ii) synthesize the major findings emerging from these studies; and (iii) identify congruencies and discrepancies in the phylogeographical histories of different species and regions. To achieve these goals, we conducted a comprehensive review of peer-reviewed literature on phylogeographic and genetic structure studies of Drosophilidae published between 1987 and 2024. After identifying and filtering relevant studies, we extracted and analyzed key information related to each topic. Overall, we have detected a straightforward predominance of studies involving species of the Drosophila genus, especially within the melanogaster, obscura, and repleta groups. Interestingly, most studies employed nuclear DNA markers, either alone or in combination with mitochondrial markers, and were conducted across more than one biogeographical region, primarily in the Palearctic and Nearctic. Thus, our synthesis underscores the importance of broader taxonomic sampling and increased attention to understudied regions to enhance our understanding of biodiversity dynamics in response to environmental changes at both local and global scales.","PeriodicalId":12557,"journal":{"name":"Genetics and Molecular Biology","volume":"49 suppl 1","pages":"e20250246"},"PeriodicalIF":1.3,"publicationDate":"2026-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12895237/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146165000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The mitogenome mutation repertoire affects progression of Parkinson's Disease. 有丝分裂基因组突变库影响帕金森病的进展。

IF 1.7 4区生物学

Genetics and Molecular Biology Pub Date : 2026-02-09 eCollection Date: 2026-01-01 DOI: 10.1590/1678-4685-GMB-2025-0098

Gustavo Barra Matos, Camille Sena Dos Santos, Letícia Cota Cavaleiro de Macêdo, Juliana Paiva Dos Santos Diniz, Tatiane Piedade de Sousa, Giovanna Chaves Cavalcante, Caio Santos Silva, Rebecca Lais da Silva Cruz, Dafne Dalledone Moura, Andrea Ribeiro-Dos-Santos, Bruno Lopes Santos-Lobato, Gilderlanio Santana de Araújo

{"title":"The mitogenome mutation repertoire affects progression of Parkinson's Disease.","authors":"Gustavo Barra Matos, Camille Sena Dos Santos, Letícia Cota Cavaleiro de Macêdo, Juliana Paiva Dos Santos Diniz, Tatiane Piedade de Sousa, Giovanna Chaves Cavalcante, Caio Santos Silva, Rebecca Lais da Silva Cruz, Dafne Dalledone Moura, Andrea Ribeiro-Dos-Santos, Bruno Lopes Santos-Lobato, Gilderlanio Santana de Araújo","doi":"10.1590/1678-4685-GMB-2025-0098","DOIUrl":"10.1590/1678-4685-GMB-2025-0098","url":null,"abstract":"Mitochondrial genome variation is a risk factor for Parkinson's disease, but its role in levodopa-induced dyskinesia remains incompletely understood. This study examines the mitochondrial mutation repertoire as a potential biomarker for levodopa-induced dyskinesia in patients with Parkinson's disease. We analyzed the mitogenome using next-generation sequencing data from 42 controls and 45 people with Parkinson's (25 without dyskinesia and 20 with dyskinesia). The mtDNA-server 2 workflow was applied for variant calling analysis. Transition and transversion rates vary during disease progression, especially in patients without levodopa-induced dyskinesia. Although the occurrence of these mutations does not follow a linear pattern, the frequency of transitions modestly increases with age. Specific coding regions (CO1, CO2, CO3, ND4, ND5, and ND6) and the regulatory region (RNR2) exhibited an enrichment of transitions and transversions in patients without dyskinesia. Additionally, we have upgraded the mtDNA-network tool (https://apps.lghm.ufpa.br/mtdna) with an integrated visual component that summarizes the mitochondrial profile in Parkinson's disease. The study highlights dynamic shifts in the mitochondrial mutation repertoire, with clinical implications for underrepresented populations, underscoring the importance of accounting for genetic characteristics across diverse groups.","PeriodicalId":12557,"journal":{"name":"Genetics and Molecular Biology","volume":"49 suppl 4","pages":"e20250098"},"PeriodicalIF":1.7,"publicationDate":"2026-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12965417/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147369152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Staphylococcal phages as agents of evolution and innovation: From gene flow to next-generation therapeutics. 葡萄球菌噬菌体作为进化和创新的媒介：从基因流到下一代疗法。

IF 1.3 4区生物学

Genetics and Molecular Biology Pub Date : 2026-02-09 eCollection Date: 2026-01-01 DOI: 10.1590/1678-4685-GMB-2025-0131

Ciro César Rossi, Felipe Castro Oliveira de Brito Teixeira, Giarlã Cunha da Silva, Monalessa Fábia Pereira, Marcia Giambiagi-deMarval

{"title":"Staphylococcal phages as agents of evolution and innovation: From gene flow to next-generation therapeutics.","authors":"Ciro César Rossi, Felipe Castro Oliveira de Brito Teixeira, Giarlã Cunha da Silva, Monalessa Fábia Pereira, Marcia Giambiagi-deMarval","doi":"10.1590/1678-4685-GMB-2025-0131","DOIUrl":"10.1590/1678-4685-GMB-2025-0131","url":null,"abstract":"Staphylococcus species include both well-known pathogens and overlooked reservoirs of antimicrobial resistance. With rising resistance rates and limited treatment options, especially for methicillin-resistant strains, interest in alternative therapies has resurged. Among them, bacteriophages (phages) are promising biological agents due to their high specificity, low toxicity, ability to disrupt biofilms, and co-evolution with bacterial hosts. This review explores the biology, pan-genomics, diversity, and therapeutic relevance of staphylococcal phages. We revisit their historical discovery and re-emergence as tools against multidrug-resistant infections, highlighting morphological features, replication strategies, and recent taxonomic updates. Genomic analyses reveal distinct clusters of genome sizes, rare presence of resistance genes, and implications of transduction, bacterial defense systems, and phage-encoded anti-defense mechanisms. Preclinical studies show broad host range and synergistic activity with diverse antimicrobial agents, while engineered phage enzymes expand therapeutic possibilities. Clinical evidence, though limited, supports safety and efficacy in compassionate-use cases and early trials targeting Staphylococcus. Finally, we examine business models translating phage innovation into applied therapies, emphasizing regulatory, logistical, and financial challenges. In this broader context, phage technologies are not just alternatives to antibiotics-they represent an opportunity for innovation in global health. Their full potential depends on coordinated actions across science, industry, and policy.","PeriodicalId":12557,"journal":{"name":"Genetics and Molecular Biology","volume":"49 suppl 1","pages":"e20250131"},"PeriodicalIF":1.3,"publicationDate":"2026-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12895238/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146165051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel protein truncating mutation of TTC8 causes Bardet-Biedl Syndrome (BBS) in a Pakistani family. 一种新的TTC8蛋白截断突变导致巴基斯坦一个家庭的Bardet-Biedl综合征（BBS）。

IF 1.3 4区生物学

Genetics and Molecular Biology Pub Date : 2026-02-09 eCollection Date: 2026-01-01 DOI: 10.1590/1678-4685-GMB-2025-0020

Sana Fatima, Dong Sun, Jianguo Han, Ming Qiu, Safeer Ahmad, Muhammad Zubair, Muhammad Zeeshan Ali, Safdar Abbas, Maria Shafiq, Muhammad Muzammal, Hadia Gul, Jabbar Khan, Shiwei Du, Muzammil Ahmad Khan

{"title":"A novel protein truncating mutation of TTC8 causes Bardet-Biedl Syndrome (BBS) in a Pakistani family.","authors":"Sana Fatima, Dong Sun, Jianguo Han, Ming Qiu, Safeer Ahmad, Muhammad Zubair, Muhammad Zeeshan Ali, Safdar Abbas, Maria Shafiq, Muhammad Muzammal, Hadia Gul, Jabbar Khan, Shiwei Du, Muzammil Ahmad Khan","doi":"10.1590/1678-4685-GMB-2025-0020","DOIUrl":"10.1590/1678-4685-GMB-2025-0020","url":null,"abstract":"Bardet-Biedl syndrome (BBS) is a rare ciliopathic disorder that segregates in an autosomal recessive manner. Genetic studies have so far identified 26 BBS-associated genes worldwide. This study analyzed a multiplex consanguineous Pakistani family with Bardet-Biedl syndrome. Genetic analysis was performed using whole-exome sequencing and Sanger sequencing. Additionally, in silico predictions were performed for functional characterization of the identified mutation. Whole exome analysis of this family identified a novel nonsense mutation [(NM_144596: exon11:c.C1047G: p.(Tyr349*)] in the 11th exon of TTC8 gene. The identified mutation presumably leads to removal of four TPR domains and C-terminus portion. Structural analyses of mutant TTC8 protein showed substantial morphologic and interactional variations, suggesting a defective role of the TTC8 protein in BBSome complex and thus its involvement in disease progression. Identification of novel mutation has expanded the mutational spectrum of TTC8. Moreover, these findings will help in genotype-phenotype association, prenatal diagnosis and genetic counseling of families at risk of BBS syndrome.","PeriodicalId":12557,"journal":{"name":"Genetics and Molecular Biology","volume":"49 1","pages":"e20250020"},"PeriodicalIF":1.3,"publicationDate":"2026-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12904352/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146194784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phylogenomic analysis of Paracidovorax citrulli strains reveals the presence of two lineages in Brazil. 对巴西瓜瓜副酸菌株的系统基因组分析显示存在两个谱系。

IF 1.7 4区生物学

Genetics and Molecular Biology Pub Date : 2026-02-06 eCollection Date: 2026-01-01 DOI: 10.1590/1678-4685-GMB-2025-0046

David F Duarte, Lucas P Lucena, Marcelo H O Gonçalves, Ana M Benko-Iseppon, Flávia Aburjaile, Vasco Azevedo, Bertram Brenig, Marco A S Gama, Elineide B Souza

{"title":"Phylogenomic analysis of Paracidovorax citrulli strains reveals the presence of two lineages in Brazil.","authors":"David F Duarte, Lucas P Lucena, Marcelo H O Gonçalves, Ana M Benko-Iseppon, Flávia Aburjaile, Vasco Azevedo, Bertram Brenig, Marco A S Gama, Elineide B Souza","doi":"10.1590/1678-4685-GMB-2025-0046","DOIUrl":"10.1590/1678-4685-GMB-2025-0046","url":null,"abstract":"Paracidovorax citrulli is the causative agent of bacterial fruit blotch in melons and watermelons. This study used comparative genomic approaches of 17 Brazilian P. citrulli strains obtained from melons and watermelons to classify them into groups I and II and try to understand their genomic differences. The genomes of P. citrulli presented general characteristics similar to those shown for the genomes of the type strain of P. citrulli and reference strains of groups I and II. A phylogenomic analysis revealed two distinct groups of P. citrulli, in which most Brazilian P. citrulli strains were grouped with the strain representing group I. CRISPR-Cas analysis revealed the presence of two proteins, Cas3 and Cas10, in all Brazilian P. citrulli genomes. In addition, we observed the presence of two plasmids (pAMC6 and pAC53) in three Brazilian P. citrulli strains, all closely related to group I. The prediction of effector proteins revealed the XopE/AvrPphe protein as a differential between the strains of groups I and II. The present study will contribute to a more detailed understanding of aspects of host-pathogen interactions and will help improve the detection of strains from these groups, thus elucidating the population dynamics of Brazilian strains of P. citrulli.","PeriodicalId":12557,"journal":{"name":"Genetics and Molecular Biology","volume":"48 4","pages":"e20250046"},"PeriodicalIF":1.7,"publicationDate":"2026-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12961662/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147289555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structural evolution of the whole mitochondrial genome and phylogenetic inference in snakes (Squamata: Serpentes), including the undescribed mitogenome of the Brazilian endemic and critically endangered pitviper Bothrops insularis. 蛇类（Squamata: Serpentes）全线粒体基因组的结构进化和系统发育推断，包括未描述的巴西特有和极度濒危的pitviper Bothrops insularis的有丝分裂基因组。

IF 1.3 4区生物学

Genetics and Molecular Biology Pub Date : 2026-01-30 eCollection Date: 2026-01-01 DOI: 10.1590/1678-4685-GMB-2024-0196

Igor Salles-Oliveira, José S L Patané, Milton Y Nishiyama-Júnior, Maria José J Silva

{"title":"Structural evolution of the whole mitochondrial genome and phylogenetic inference in snakes (Squamata: Serpentes), including the undescribed mitogenome of the Brazilian endemic and critically endangered pitviper Bothrops insularis.","authors":"Igor Salles-Oliveira, José S L Patané, Milton Y Nishiyama-Júnior, Maria José J Silva","doi":"10.1590/1678-4685-GMB-2024-0196","DOIUrl":"10.1590/1678-4685-GMB-2024-0196","url":null,"abstract":"Evolutionary analyses of mitogenomes have provided insights into species evolution and conservation. Studies on snakes revealed a higher diversity especially involving variation in tRNA clusters. However, despite the increase in assemblies in databases, the available information for Brazilian species remains scarce in mitogenomic surveys. Based on this, we sequenced and provided the first description of the mitogenome of Bothrops insularis, a critically endangered taxon. We also used the B. insularis assembly and 128 molecules available in databases up to March 2022 to explore rearrangements and evolution of the mtDNA under a phylogenomic perspective. Comparative analyses revealed 24 mitotypes due to rearrangements within four tRNA clusters and within control regions. Mitotype 1 (M1) and Mitotype 2 (M2) are restricted to Scolecophidia and M3 is highly distributed within Alethinophidia. In addition, the M3 is suggested as the most likely ancestral mitotype during the mitogenome evolution. Regarding Bothrops, we recovered the same mitotype for B. jararaca and B. insularis, which structure differs from other Bothrops species. Nucleotide variation suggests 1.5% divergence between B. jararaca and B. insularis. Therefore, we suggest that B. insularis can be considered an evolutionary significant unit, and the data generated herein can be valuable for insights into genome evolution and conservation.","PeriodicalId":12557,"journal":{"name":"Genetics and Molecular Biology","volume":"48 4","pages":"e20240196"},"PeriodicalIF":1.3,"publicationDate":"2026-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12893195/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146156686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phyllosphere microbiomes uncovered: Research trends, geographic disparities, and key microbial players. 层球微生物群揭示：研究趋势、地理差异和关键微生物参与者。

IF 1.3 4区生物学

Genetics and Molecular Biology Pub Date : 2026-01-23 eCollection Date: 2026-01-01 DOI: 10.1590/1678-4685-GMB-2025-0083

Isabel Cristina Cadavid, Érika Frydrych Capelari, Caroline Salvati, Rogerio Margis

{"title":"Phyllosphere microbiomes uncovered: Research trends, geographic disparities, and key microbial players.","authors":"Isabel Cristina Cadavid, Érika Frydrych Capelari, Caroline Salvati, Rogerio Margis","doi":"10.1590/1678-4685-GMB-2025-0083","DOIUrl":"10.1590/1678-4685-GMB-2025-0083","url":null,"abstract":"In recent years, significant efforts have been made to understand the phyllosphere microbiome. To clarify current research trends and identify knowledge gaps, we conducted a systematic review of the literature and investigated the methodologies used to analyze microbiome communities associated with plants, along with the objectives of these studies. Applying systematic review principles, we assessed 333 reports from the Web of Science database for eligibility. Articles were included if they presented original research on microbiomes associated with phyllosphere tissues identified by next-generation sequencing. Of these, 268 reports, published from 2009 to March 2025, were retrieved. These reports were used to extract data in a controlled and methodical manner. The analyses identified the most frequently studied plant species and primary tissues, the geographical locations sampled, the variables investigated in the phyllosphere microbiome, and the methodologies and tools employed. A comparison of the number of studies on below-ground (n=1562) versus above-ground tissue (n=375) underscores the relatively unexplored nature of above-ground research. We notice a surprisingly low number of studies from the Southern Hemisphere. Additionally, through data mining, we identify the most dominant bacteria and fungi reported in phyllosphere studies. Based on these findings, we offer recommendations for future research on the phyllosphere.","PeriodicalId":12557,"journal":{"name":"Genetics and Molecular Biology","volume":"49Suppl 1 Suppl 1","pages":"e20250083"},"PeriodicalIF":1.3,"publicationDate":"2026-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12893196/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146164967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gene expression analysis for feed efficiency trait in liver tissue of lactating Girolando cows. 泌乳吉兰多奶牛肝组织饲料效率性状的基因表达分析。

IF 1.3 4区生物学

Genetics and Molecular Biology Pub Date : 2026-01-19 eCollection Date: 2026-01-01 DOI: 10.1590/1678-4685-GMB-2025-0036

Daniele Ribeiro de Lima Reis Faza, Mariana Magalhães Campos, Thierry Ribeiro Tomich, Fernanda Samarini Machado, Luiz Gustavo Ribeiro Pereira, Robert Domingues, Ana Luiza Franco, Marta Fonseca Martins, João Cláudio do Carmo Panetto, Marcos Vinicius Gualberto Barbosa da Silva, Wanessa Araújo Carvalho, Marco Antonio Machado

{"title":"Gene expression analysis for feed efficiency trait in liver tissue of lactating Girolando cows.","authors":"Daniele Ribeiro de Lima Reis Faza, Mariana Magalhães Campos, Thierry Ribeiro Tomich, Fernanda Samarini Machado, Luiz Gustavo Ribeiro Pereira, Robert Domingues, Ana Luiza Franco, Marta Fonseca Martins, João Cláudio do Carmo Panetto, Marcos Vinicius Gualberto Barbosa da Silva, Wanessa Araújo Carvalho, Marco Antonio Machado","doi":"10.1590/1678-4685-GMB-2025-0036","DOIUrl":"10.1590/1678-4685-GMB-2025-0036","url":null,"abstract":"The selection of high feed efficiency (FE) animals impacts sustainability and profitability of beef and dairy cattle production systems. An approach to investigate the mechanisms of FE involves analyzing gene expression profile in liver. This study used residual feed intake as a metric of FE to select 10 Gir x Holstein crossbred cows (Girolando F1) divided into high (HE) and low (LE) FE groups. Hepatic biopsies were used for differential gene expression investigation using RNA-seq analyses which revealed 20,787 known genes mapped accordingly to the bovine reference genome. The comparison of HE and LE revealed 149 significantly differentially expressed genes (DEG), 41 up-regulated, and 108 down-regulated in the LE group. Among DEG, some stood out as potential candidate genes, including DLK1, CACNG4, SLC2A12, SLC26A4, DUOX2, and DUOXA2. Functional enrichment analyses showed pathways that potentially influence FE, such as the negative regulation of leukocyte migration, regulation of calcium channel activity, negative regulation of cell migration and adhesion, extracellular matrix (ECM) organization, and thyroid hormone synthesis. ECM composition and immune system roles were also highlighted. These results could help understanding the mechanisms related to FE in dairy cattle and the development of selection strategies to improve this trait.","PeriodicalId":12557,"journal":{"name":"Genetics and Molecular Biology","volume":"48 4","pages":"e20250036"},"PeriodicalIF":1.3,"publicationDate":"2026-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12814931/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146003470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Updating the frequency of CCR5Δ32 in Brazil: Descriptive analysis of malaria cases and controls from Acre State, Amazon region. 更新巴西CCR5Δ32的频率：亚马逊地区阿克里州疟疾病例和控制的描述性分析。

IF 1.3 4区生物学

Genetics and Molecular Biology Pub Date : 2026-01-16 eCollection Date: 2026-01-01 DOI: 10.1590/1678-4685-GMB-2025-0056

Marina Ziliotto, Joel Henrique Ellwanger, Jamille Gregório Dombrowski, Claudio Romero Farias Marinho, Alessandra Pontillo, José Artur Bogo Chies

引用次数: 0