Genetics and Molecular Biology最新文献_第7页

Conserved gene clusters in entomopathogenic filamentous fungi. 昆虫病原丝状真菌中的保守基因簇。

IF 1.7 4区生物学

Genetics and Molecular Biology Pub Date : 2026-04-17 eCollection Date: 2026-01-01 DOI: 10.1590/1678-4685-GMB-2025-0168

Alexandra de Azevedo da Rocha, Charley Christian Staats

{"title":"Conserved gene clusters in entomopathogenic filamentous fungi.","authors":"Alexandra de Azevedo da Rocha, Charley Christian Staats","doi":"10.1590/1678-4685-GMB-2025-0168","DOIUrl":"10.1590/1678-4685-GMB-2025-0168","url":null,"abstract":"Entomopathogenic filamentous fungi from the Order Hypocreales are studied for biological pest control due to their ability to infect arthropods. Their biotechnological potential lies in the production of secondary metabolites (SMs), encoded by biosynthetic gene clusters (BGCs), which are crucial for the fungal life cycle and infection. Due to the different roles played by SMs in fungi, the in-depth study of these molecules has increased over the last years. Considering the proven biotechnological importance of species of the genus Beauveria have already shown, we performed an in-silico analysis of BGCs of the species from the order Hypocreales to uncover potential conserved pathways and find new candidates for biological pest control. A total of 295 genome sequences were analyzed using antiSMASH, allowing the identification of 12,968 BGCs. Conservation analysis was performed using BiG-SCAPE, which could group these BGCs into 2,127 biosynthetic gene families. Despite the presence of conserved gene clusters, especially within the same genus, when the comparison of BGCs was performed within the order, a large part of them is orphaned, highlighting the great diversity of BGCs and consequently the chemodiversity of fungal species. Our approach helps to uncover new molecules that may provide new biotechnological tools.","PeriodicalId":12557,"journal":{"name":"Genetics and Molecular Biology","volume":"49 1","pages":"e20250168"},"PeriodicalIF":1.7,"publicationDate":"2026-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13123250/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147768805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Therapeutic potential of Brazilian medicinal plants and their metabolites. 巴西药用植物及其代谢产物的治疗潜力。

IF 1.7 4区生物学

Genetics and Molecular Biology Pub Date : 2026-04-10 eCollection Date: 2026-01-01 DOI: 10.1590/1678-4685-GMB-2025-0222

Juliana Mara Serpeloni, Fabio Vieira Dos Santos, Katiuska Tuttis, Ilce Mara de Syllos Cólus

{"title":"Therapeutic potential of Brazilian medicinal plants and their metabolites.","authors":"Juliana Mara Serpeloni, Fabio Vieira Dos Santos, Katiuska Tuttis, Ilce Mara de Syllos Cólus","doi":"10.1590/1678-4685-GMB-2025-0222","DOIUrl":"10.1590/1678-4685-GMB-2025-0222","url":null,"abstract":"Exploring natural products is essential for identifying new bioactive substances with unique molecular structures and mechanisms of action. Successful experiences in identifying and developing chemotherapeutic agents from plant-derived sources have inspired the search for new strategies and alternative treatments for various diseases. Today, more than half of the anticancer drugs in clinical use are derived, directly or indirectly, from natural products. In this review, we present findings from studies on medicinal plants conducted at the Laboratory of Mutagenesis and Oncogenetics, in collaboration with other research groups from the Program Biota/FAPESP, aimed at evaluating their safety for widespread use. Our research team has conducted extensive studies over approximately 25 years, assessing 65 crude plant extracts from 31 species and 8 isolated phytochemicals. Most of these species belong to the Melastomataceae family, followed by the Malpighiaceae family. Among the phytochemicals, flavonoids, diterpenes, indolinones, and alkaloids are the most common. In this comprehensive review, we examine the biological effects identified and their potential therapeutic applications, particularly in the context of anticancer strategies. Our goal is to highlight the pharmacological potential of compounds derived from these plants, thereby supporting the development of drugs that can be effectively utilized in clinical practice.","PeriodicalId":12557,"journal":{"name":"Genetics and Molecular Biology","volume":"49Suppl 1 Suppl 1","pages":"e20250222"},"PeriodicalIF":1.7,"publicationDate":"2026-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13072110/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147672191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic insights into the peoples who shaped the American continent. 对塑造美洲大陆的民族的基因研究。

IF 1.7 4区生物学

Genetics and Molecular Biology Pub Date : 2026-04-03 eCollection Date: 2026-01-01 DOI: 10.1590/1678-4685-GMB-2025-0244

Gustavo Medina Tavares, Bruna Oliveira Missaggia, Thaynara Lima, Mariana Marcano-Ruiz, Maria Thereza Schmitt Mesquita, Guilherme Baldo, Márcio Dorn, Tábita Hünemeier, Maria Cátira Bortolini

{"title":"Genetic insights into the peoples who shaped the American continent.","authors":"Gustavo Medina Tavares, Bruna Oliveira Missaggia, Thaynara Lima, Mariana Marcano-Ruiz, Maria Thereza Schmitt Mesquita, Guilherme Baldo, Márcio Dorn, Tábita Hünemeier, Maria Cátira Bortolini","doi":"10.1590/1678-4685-GMB-2025-0244","DOIUrl":"10.1590/1678-4685-GMB-2025-0244","url":null,"abstract":"The initial peopling of America left a deep genetic legacy in Indigenous peoples and their admixed descendants. This narrative review recenters studies involving Indigenous populations and, inspired by the work of Francisco M. Salzano and Darcy Ribeiro's historical and cultural framework, adopts the working notions of \"witness,\" \"introduced,\" \"transplanted,\" and \"new\" genetic signatures. We first clarify terminology to avoid neocolonial bias, using America to denote the continent and Native American to refer to all Indigenous peoples of America, and then synthesize the literature on initial peopling, post-contact demography, and natural selection, with particular emphasis on Brazil. We also present an illustrative example drawn from ongoing research conducted by our group, using genome editing to investigate a candidate adaptive allele in the context of high-altitude adaptation. Finally, we connect evolutionary history to contemporary health, highlighting mitonuclear interactions, dietary transitions, and pathogen exposures that may modulate disease risk, with implications for precision public health. Collectively, this review showcases ancestry-aware approaches tailored to Native American contexts and demonstrates why models developed elsewhere should not be uncritically extrapolated to America, advancing a continent-wide, Brazil-anchored perspective on Indigenous resilience and scientific significance.","PeriodicalId":12557,"journal":{"name":"Genetics and Molecular Biology","volume":"49Suppl 1 Suppl 1","pages":"e20250244"},"PeriodicalIF":1.7,"publicationDate":"2026-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13063108/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147627547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Regulation of long non-coding RNAs by essential oils: Therapeutic implications in human diseases. 精油对长链非编码rna的调控：对人类疾病的治疗意义。

IF 1.7 4区生物学

Genetics and Molecular Biology Pub Date : 2026-04-03 eCollection Date: 2026-01-01 DOI: 10.1590/1678-4685-GMB-2025-0181

Lívia da Cunha Agostini, Mariane Ster da Silva Teixeira, Glenda Nicioli da Silva

引用次数: 0

The dual role of sertraline: From antidepressant to antifungal drug with broad metabolic disruption. 舍曲林的双重作用：从抗抑郁药到具有广泛代谢紊乱的抗真菌药。

IF 1.7 4区生物学

Genetics and Molecular Biology Pub Date : 2026-04-03 eCollection Date: 2026-01-01 DOI: 10.1590/1678-4685-GMB-2025-0183

Mayara I G Azevedo, Maria J Santos-Saboya, João Neves-da-Rocha, Monise F Petrucelli, Nilce M Martinez-Rossi, Antonio Rossi

引用次数: 0

Expression Analysis of the orphan receptors GPR161, GPR132, GPR20, and GPR139 in patients with cervicitis and low-grade, and high-grade squamous intraepithelial lesions. 孤儿受体GPR161、GPR132、GPR20和GPR139在宫颈炎和低级别、高级别鳞状上皮内病变患者中的表达分析

IF 1.7 4区生物学

Genetics and Molecular Biology Pub Date : 2026-03-30 eCollection Date: 2026-01-01 DOI: 10.1590/1678-4685-GMB-2025-0191

Jessica E Rodríguez, Javier González-Ramírez, Alina Barquet-Nieto, Gladys E Ramírez-Rosales, Lilly G Cruz-Izaguirre, Brenda J Reyes-Solorio, Rosa P Cruz-Nieves, Edgar Torres-Maravilla, Armando Ruiz-Hernández

{"title":"Expression Analysis of the orphan receptors GPR161, GPR132, GPR20, and GPR139 in patients with cervicitis and low-grade, and high-grade squamous intraepithelial lesions.","authors":"Jessica E Rodríguez, Javier González-Ramírez, Alina Barquet-Nieto, Gladys E Ramírez-Rosales, Lilly G Cruz-Izaguirre, Brenda J Reyes-Solorio, Rosa P Cruz-Nieves, Edgar Torres-Maravilla, Armando Ruiz-Hernández","doi":"10.1590/1678-4685-GMB-2025-0191","DOIUrl":"10.1590/1678-4685-GMB-2025-0191","url":null,"abstract":"Cervical cancer is one of the most common types of carcinomas causing morbidity and mortality in women worldwide, primarily caused by high-risk human papillomavirus (hrHPV). However, some cervical tumors, particularly adenocarcinomas, may develop independently of HPV. Chronic cervicitis, often linked to bacterial infections, increases the risk of developing squamous low and high-grade intraepithelial lesions (LSIL and HSIL, respectively) and cervical cancer. Cancer-associated inflammation activates key signaling pathways (NF-κB, STAT, and FOXO), regulating orphan G protein-coupled receptors (oGPCRs) implicated in cancer. Notably, GPR161, GPR132, GPR20, and GPR139 play roles in various tumors. This study analyzed cervical tissue samples from 45 women undergoing colposcopy at UNEME Oncology in Mexicali, Baja California. Patient data, including reproductive history and lifestyle factors, were collected. The histopathological analysis identified 31.1% with cervicitis, 40% with LSIL, and 28.8% with HSIL. mRNA expression of orphan GPRs varied among groups, with GPR132 and GPR20 significantly elevated in LSIL samples (p < 0.05), while GPR139 expression was reduced in LSIL (p < 0.05). No significant difference was found for GPR161. The differential expression of oGPCRs in cervical tissue suggests their involvement in the progression of LSIL and HSIL, offering insights into novel therapeutic targets.","PeriodicalId":12557,"journal":{"name":"Genetics and Molecular Biology","volume":"49 1","pages":"e20250191"},"PeriodicalIF":1.7,"publicationDate":"2026-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13037469/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147581005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Toward the establishment of best practice guidelines for human research in Genomic Medicine in Brazil. 在巴西为人类基因组医学研究建立最佳实践指南。

IF 1.7 4区生物学

Genetics and Molecular Biology Pub Date : 2026-03-16 eCollection Date: 2026-01-01 DOI: 10.1590/1678-4685-GMB-2024-0230

Fernanda Sperb Ludwig, Osvaldo Artigalás, Nureyev Ferreira Rodrigues, Patricia Ashton Prolla, Ursula da Silveira Matte, Jonas Alex Morales Saute

{"title":"Toward the establishment of best practice guidelines for human research in Genomic Medicine in Brazil.","authors":"Fernanda Sperb Ludwig, Osvaldo Artigalás, Nureyev Ferreira Rodrigues, Patricia Ashton Prolla, Ursula da Silveira Matte, Jonas Alex Morales Saute","doi":"10.1590/1678-4685-GMB-2024-0230","DOIUrl":"10.1590/1678-4685-GMB-2024-0230","url":null,"abstract":"Many countries lack their guidelines for best practices in genomic medicine, whether for clinical practice or research. For the construction of these national guidelines or institutional policies in human genomics research several key criteria must be observed. The determination of variant pathogenicity has become more refined, with specific global recommendations being adopted. A well-conducted consent process, managed by qualified professionals, ensures the protection of research participants' rights. Pre-established sequencing and data analysis parameters are crucial for ensuring reliable results, and clear guidelines must be provided on whether or not to disclose findings to participants. Data storage remains a challenge due to both the size and sensitivity of the generated files. This work discusses these critical aspects and represents an initiative to propose the best practice recommendations for genomic medicine in human research. These recommendations were developed by a working group of experts and were further refined through discussions with members of the scientific community in genetics and genomic medicine.","PeriodicalId":12557,"journal":{"name":"Genetics and Molecular Biology","volume":"49 1","pages":"e20240230"},"PeriodicalIF":1.7,"publicationDate":"2026-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13016436/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147491159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of CRISPR-Cas systems in the Haemophilus genus CRISPR-Cas in Haemophilus spp. 嗜血杆菌属CRISPR-Cas系统的表征。

IF 1.7 4区生物学

Genetics and Molecular Biology Pub Date : 2026-03-16 eCollection Date: 2026-01-01 DOI: 10.1590/1678-4685-GMB-2025-0166

Ying Huang, Xinchao Yi, Xue Yang, Chao Li, Yuan Li, Zufeng Ye, Jun He

{"title":"Characterization of CRISPR-Cas systems in the Haemophilus genus CRISPR-Cas in Haemophilus spp.","authors":"Ying Huang, Xinchao Yi, Xue Yang, Chao Li, Yuan Li, Zufeng Ye, Jun He","doi":"10.1590/1678-4685-GMB-2025-0166","DOIUrl":"10.1590/1678-4685-GMB-2025-0166","url":null,"abstract":"Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR-associated (Cas) system constitutes a crucial adaptive defense mechanism in prokaryotes against foreign genetic elements. Although CRISPR-Cas systems have been characterized in numerous bacteria, the architecture and function of these systems in the Haemophilus genus remain poorly understood. This study aims to analyze CRISPR-Cas systems in 138Haemophilusstrains and investigate their function, particularly in relation to virulence factors. Results revealed that CRISPR-Cas systems were identified in 31.88% of the Haemophilusstrains. Subtype I-C was the most prevalent, followed by subtypes II-C and III-A. Repeat sequences and thecas1gene were highly conserved within the same subtype. 29.62% of spacer sequences exhibited homology to plasmids or bacteriophages. phiMHaA1 was an important target of the CRISPR-Cas system in Haemophilusgenus. The protospacer adjacent motif sequences (PAM) were determined to be 5'-TTC-3' for subtype I-C and 5'-TTT-3' for subtype II-C. Comparative analysis of virulence genes showed that CRISPR-positive strains carried more ompP2 than CRISPR-negative strains, while the distribution of hmw2C and hmw1C exhibited an opposite trend. These findings provide novel insights into the diversity and function of CRISPR-Cas systems inHaemophilusgenus and propose potential strategies for attenuating the impact ofHaemophilusvirulence factors.","PeriodicalId":12557,"journal":{"name":"Genetics and Molecular Biology","volume":"49 1","pages":"e20250166"},"PeriodicalIF":1.7,"publicationDate":"2026-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13016550/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147491100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

From natural theology to the extended synthesis: Historical milestones and conceptual expansions in evolutionary biology. 从自然神学到扩展综合：进化生物学的历史里程碑和概念扩展。

IF 1.7 4区生物学

Genetics and Molecular Biology Pub Date : 2026-02-13 eCollection Date: 2026-01-01 DOI: 10.1590/1678-4685-GMB-2025-0179

Francisco Prosdocimi, Marco Garbin, Francesco Dondero

{"title":"From natural theology to the extended synthesis: Historical milestones and conceptual expansions in evolutionary biology.","authors":"Francisco Prosdocimi, Marco Garbin, Francesco Dondero","doi":"10.1590/1678-4685-GMB-2025-0179","DOIUrl":"10.1590/1678-4685-GMB-2025-0179","url":null,"abstract":"This article explores the historical development of evolutionary biology-from Natural Theology to the Modern Synthesis (MS)-and the ongoing debate around the Extended Evolutionary Synthesis (EES). Over the past 2,500 years, evolutionary thinking has emerged from the interplay between empirical discoveries and dominant philosophical paradigms. Beginning with Aristotle and Saint Augustine, we trace how Darwin and Wallace introduced a scientific framework grounded in natural mechanisms. In the early 20th century, the MS unified Mendelian genetics and Darwinian selection, forming a gene-centered model of evolution focused on mutations and population dynamics. In recent decades, discoveries in epigenetics, phenotypic plasticity, symbiosis, niche construction, and cultural inheritance have challenged the explanatory scope of MS. The EES seeks to incorporate these processes not by discarding Darwinian principles, but by reinterpreting them through a systems biology lens. This mostly represents a conceptual shift in focus: from linear, gene-driven causality to multilevel, reciprocal, and environmentally embedded dynamics. While gaining traction, the EES has been criticized for its lack of formal models and predictive frameworks, remaining a contested proposal. Ultimately, evolutionary biology continues to evolve as a powerful scientific tradition, driven by humanity's enduring quest to understand the origins and evolution of life on Earth.","PeriodicalId":12557,"journal":{"name":"Genetics and Molecular Biology","volume":"49 suppl 2","pages":"e20250179"},"PeriodicalIF":1.7,"publicationDate":"2026-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12961663/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147289597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Soybean genetic engineering in Brazil and South America - A review. 巴西和南美大豆基因工程研究综述。

IF 1.7 4区生物学

Genetics and Molecular Biology Pub Date : 2026-02-13 eCollection Date: 2026-01-01 DOI: 10.1590/1678-4685-GMB-2025-0007

Renata Fuganti-Pagliarini, Mayla Daiane Correa Molinari, João Matheus Kafer, Alexandre Lima Nepomuceno, Liliane Marcia Mertz Henning, Sérgio Hermínio Brommonschenkel, Larissa Goulart Zanardo, Maria Helena Bodanese-Zanettini, Elibio Rech

{"title":"Soybean genetic engineering in Brazil and South America - A review.","authors":"Renata Fuganti-Pagliarini, Mayla Daiane Correa Molinari, João Matheus Kafer, Alexandre Lima Nepomuceno, Liliane Marcia Mertz Henning, Sérgio Hermínio Brommonschenkel, Larissa Goulart Zanardo, Maria Helena Bodanese-Zanettini, Elibio Rech","doi":"10.1590/1678-4685-GMB-2025-0007","DOIUrl":"10.1590/1678-4685-GMB-2025-0007","url":null,"abstract":"Soybean like other commodities brings important income to producer countries. In Brazil, the picture is not different. Thus, due to its economic significance and considering all field challenges, that impose abiotic and biotic constraints to improve yield, research efforts have focused on solving problems by applying biotechnological tools. In this review, a description of genetic engineering being developed in Brazil for soybean crop is addressed, including research in tolerance to abiotic conditions such as drought; to biotic factors such as insects, nematodes and fungal among other categories; as well as the use of soybean as bio-factories. All commercially available soybean events in Brazil and South American countries, for insect resistance and herbicide tolerance are also discussed. An overview of the Brazilian regulatory framework and South American countries for Biotech products is presented as well as the future perspectives for soybean genetic engineering in Brazil. In addition, a list of papers, from 2008 to the present, showing the state of the art of genetic engineering of soybean and by-products in Brazil is made available. The same search on the literature for South American countries did not return papers published on the genetic engineering of soybean and by-products. Although South American countries did not develop science on GM soybean, a search on GM varieties being sowed indicated that these countries adopted GM technology, due to all the advantages that these lines offer.","PeriodicalId":12557,"journal":{"name":"Genetics and Molecular Biology","volume":"49 1","pages":"e20250007"},"PeriodicalIF":1.7,"publicationDate":"2026-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12965416/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147369191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0