General physiology and biophysics最新文献_第2页

The ex vivo effects of hypoxanthine-tricyclano, a synthetic adenosine analogue, on rat left and right atria. 次黄嘌呤-三环（一种合成腺苷类似物）对大鼠左心房和右心房的体内外影响。

IF 1.3 4区生物学

General physiology and biophysics Pub Date : 2024-11-01 DOI: 10.4149/gpb_2024033

Ignac Ovari, Gabor Viczjan, Miklos Bege, Aniko Borbas, Pal Herczegh, Judit Zsuga, Zoltan Papp, Zoltan Szilvassy, Bela Juhasz, Rudolf Gesztelyi, Tamas Erdei

{"title":"The ex vivo effects of hypoxanthine-tricyclano, a synthetic adenosine analogue, on rat left and right atria.","authors":"Ignac Ovari, Gabor Viczjan, Miklos Bege, Aniko Borbas, Pal Herczegh, Judit Zsuga, Zoltan Papp, Zoltan Szilvassy, Bela Juhasz, Rudolf Gesztelyi, Tamas Erdei","doi":"10.4149/gpb_2024033","DOIUrl":"https://doi.org/10.4149/gpb_2024033","url":null,"abstract":"Hypoxanthine-tricyclano is a synthetic adenosine analogue, in which adenine and ribose have been replaced by hypoxanthine and a morpholino-derived tricyclic moiety, respectively. We investigated whether hypoxanthine-tricyclano could influence atrial inotropy and/or chronotropy, two important functions regulated by the A1 receptor, the main adenosine receptor type of the supraventricular myocardium. Paced left atria and spontaneously beating right atria, isolated from male, 30-35 weeks old, Wistar rats, were used. The ino- and chronotropic effects of adenosine and hypoxanthine-tricyclano (separately and together) were assessed in the absence and presence of 8-cyclopentyl-1,3-dipropylxanthine (CPX), a selective, orthosteric, reversible A1 adenosine receptor antagonist. We found that adenosine exerted a strong negative inotropic effect (similar in left and right atria). However, hypoxanthine-tricyclano elicited a moderate positive inotropic effect (also similar in all atria). In right atria, adenosine evoked a robust negative chronotropic effect, whereas hypoxanthine-tricyclano produced a slight positive chronotropy. CPX blunted the effects of both adenosine and hypoxanthine-tricyclano, although this antagonism was strong (and significant) for adenosine, while smaller (and non-significant) for hypoxanthine-tricyclano. Both effects of hypoxanthine-tricyclano were easily surmountable with adenosine. Thus, hypoxanthine-tricyclano may act as a week, orthosteric, reversible, inverse and low-affinity agonist of the A1 receptor, although alternative mechanisms of action cannot be excluded.","PeriodicalId":12514,"journal":{"name":"General physiology and biophysics","volume":"43 6","pages":"577-584"},"PeriodicalIF":1.3,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142709823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Silencing lncRNA SIX3OS1 mitigates inflammation and apoptosis in post-stroke cognitive impairment via miR-511-3p. 沉默 lncRNA SIX3OS1 可通过 miR-511-3p 减轻中风后认知障碍中的炎症和细胞凋亡。

IF 1.3 4区生物学

General physiology and biophysics Pub Date : 2024-11-01 DOI: 10.4149/gpb_2024035

Junsheng Zeng, Fen Yang, Hui Xiao, Wei Zhao, Kangping Song, Yan Liu, Te Wang

{"title":"Silencing lncRNA SIX3OS1 mitigates inflammation and apoptosis in post-stroke cognitive impairment via miR-511-3p.","authors":"Junsheng Zeng, Fen Yang, Hui Xiao, Wei Zhao, Kangping Song, Yan Liu, Te Wang","doi":"10.4149/gpb_2024035","DOIUrl":"https://doi.org/10.4149/gpb_2024035","url":null,"abstract":"The present study aimed to explore the expression and molecular mechanisms of lncRNA SIX3OS1 in post-stroke cognitive impairment (PSCI). Middle cerebral artery occlusion (MCAO) and oxygen-glucose deprivation/reoxygenation (OGD/R) were applied to establish an in vitro and in vivo model of PSCI. RT-qPCR was conducted to examine the mRNA levels of SIX3OS1, miR-511-3p, and RBP4. Morris water maze test was used to evaluate spatial learning and memory ability. Cell viability and apoptosis were examined by CCK-8 and flow cytometry. The secretion level of inflammatory factors was analyzed by ELISA. DLR and RIP assay were performed to validate the target relationship. In MCAO rats and OGD/R-induced cells, SIX3OS1 and RBP4 levels were significantly elevated, while miR-511-3p was reduced. miR-511-3p targets SIX3OS1 and RBP4. Compared with the sham, the spatial learning and memory ability of MCAO rats were decreased, but the silencing of SIX3OS1 could restore them, but this restoration was partially impaired by lowing of miR-511-3p. Silencing of SIX3OS1 enhanced OGD/R-induced SH-SY5Y cell viability and inhibited apoptosis and inflammatory factor secretion, but they were both attenuated by the lowing of miR-511-3p. Silencing of SIX3OS1 can protect PSCI via targeting miR-511-3p to promote cell viability and inhibit apoptosis and inflammation.","PeriodicalId":12514,"journal":{"name":"General physiology and biophysics","volume":"43 6","pages":"567-576"},"PeriodicalIF":1.3,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142709819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Complexity of electrodermal activity to mental stress is changed during adolescent age-period. 皮电活动对精神压力的复杂性在青少年时期发生了变化。

IF 1.3 4区生物学

General physiology and biophysics Pub Date : 2024-11-01 DOI: 10.4149/gpb_2024027

Zuzana Visnovcova, Nikola Ferencova, Ingrid Tonhajzerova

{"title":"Complexity of electrodermal activity to mental stress is changed during adolescent age-period.","authors":"Zuzana Visnovcova, Nikola Ferencova, Ingrid Tonhajzerova","doi":"10.4149/gpb_2024027","DOIUrl":"https://doi.org/10.4149/gpb_2024027","url":null,"abstract":"Complexity characterizes behaviour of all physiological systems whose components interact in multiple ways usually quantified by entropy techniques. However, complexity analysis regarding electrodermal activity (EDA)-related sympathetic cholinergic nervous system is rare. Thus, we aimed to study EDA dynamics complexity changes from aspect of various embedding dimensions (m) and timescales (τ) (sample entropy (SampEn) with m ∈ <2,7>, and multiscale entropy (MSE) in τ ∈ <1,20>) in association with traditionally used EDA indices (skin conductance level (SCL) and non-specific skin conductance responses (NS.SCRs)) to mental stress (mental arithmetic test - MAT) in healthy participants at critical adolescent age. The cohort (total group) consisted of 60 adolescents (17.5 ± 0.5 yrs) divided into three groups: Group-1: early (13.1 ± 0.3 yrs), Group-2: middle (16.6 ± 0.2 yrs) and Group-3: late (22.9 ± 0.1 yrs) adolescence. SampEn (m > 2) and MSE (for all τ) were significantly higher during MAT than baseline in total group and Group-2 (p < 0.05). Index MSE for all τ was significantly higher during MAT than baseline in total group, and Group-2; for τ ∈ <2,13> in Group-1 (p < 0.05). Additionally, while SCL was significantly higher during MAT than baseline in all groups, NS.SCRs was lower during stress only in Group-3 (p < 0.05). In conclusion, this study revealed distinct EDA complexity characteristics in individual examined groups indicating importance of complexity evaluation in stress-related sympathetic regulatory mechanisms within individual adolescent age ranges.","PeriodicalId":12514,"journal":{"name":"General physiology and biophysics","volume":"43 6","pages":"499-510"},"PeriodicalIF":1.3,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142709809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of hypoxia and KCl depolarization in autofluorescence and ROS changes at the hippocampal CA3 area. 缺氧和 KCl 去极化对海马 CA3 区自荧光和 ROS 变化的影响

IF 1.3 4区生物学

General physiology and biophysics Pub Date : 2024-11-01 DOI: 10.4149/gpb_2024005

Marta Ig Batista, Carla Mf Miranda, Sofia M Figueiredo, Morgana Bosio, João L Alves, Marta S Sousa, Fernando Ds Sampaio-Dos-Aidos, Carlos M Matias, Rosa M Quinta-Ferreira, M Emilia Quinta-Ferreira

{"title":"Effect of hypoxia and KCl depolarization in autofluorescence and ROS changes at the hippocampal CA3 area.","authors":"Marta Ig Batista, Carla Mf Miranda, Sofia M Figueiredo, Morgana Bosio, João L Alves, Marta S Sousa, Fernando Ds Sampaio-Dos-Aidos, Carlos M Matias, Rosa M Quinta-Ferreira, M Emilia Quinta-Ferreira","doi":"10.4149/gpb_2024005","DOIUrl":"https://doi.org/10.4149/gpb_2024005","url":null,"abstract":"The increasing incidence of neurodegenerative and other diseases is considered to involve an excessive production of reactive oxygen species (ROS). Water supplies are often characterized by excessive organic waste that is decomposed by bacteria, using dissolved oxygen, leading to oxygen depletion. The potassium content of these waters may also affect negatively the mitochondrial metabolism and cellular ROS formation. This work focused on characterizing mitochondrial autofluorescence changes, with flavoprotein origin, and fluorescence ROS signals measured using the 2',7'-dichlorodihydrofluorescein diacetate indicator H2DCFDA. All signals were evoked by hypoxia or by the depolarizing agent KCl (20 mM), at the hippocampal mossy fiber synapses of CA3 area. It was observed that both hypoxia and KCl-induced depolarization elicited a small rise in the autofluorescence and ROS changes. The hypoxia-induced signals were maintained upon normal reoxygenation, but of those evoked by KCl, the autofluorescence signals recovered during washout, while the ROS changes were irreversible.","PeriodicalId":12514,"journal":{"name":"General physiology and biophysics","volume":"43 6","pages":"585-592"},"PeriodicalIF":1.3,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142709811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

TGF-β1 inhibits apoptosis of cardiomyocytes H9c2 by regulating autophagy and ERK pathway. TGF-β1 通过调节自噬和 ERK 通路抑制心肌细胞 H9c2 的凋亡

IF 1.3 4区生物学

General physiology and biophysics Pub Date : 2024-11-01 DOI: 10.4149/gpb_2024030

Yifei Liu, Siyu Lin, Jianzhong Wang, Jianli Jiang, Aihua Shu, Mi Zhou

{"title":"TGF-β1 inhibits apoptosis of cardiomyocytes H9c2 by regulating autophagy and ERK pathway.","authors":"Yifei Liu, Siyu Lin, Jianzhong Wang, Jianli Jiang, Aihua Shu, Mi Zhou","doi":"10.4149/gpb_2024030","DOIUrl":"https://doi.org/10.4149/gpb_2024030","url":null,"abstract":"This study aimed to explore the expression and mechanism of transforming growth factor β1 (TGF-β1) in oxygen glucose deprivation reperfusion (OGD/R)-induced ischemia/reperfusion (I/R) injury. An OGD/R model was established in cardiomyocytes H9c2, resulting in upregulation of Beclin-1 and LC3II/LC3I expression. Upon overexpression of TGF-β1, the viability of OGD/R-induced H9c2 cells was enhanced, while apoptosis was suppressed by downregulating Bax and upregulating Bcl-2. Additionally, TGF-β1 overexpression promoted autophagy in OGD/R-induced H9c2 cells by further upregulating the levels of Beclin-1 and LC3II/LC3I. Importantly, treatments with 3-methyladenine (3-MA), an autophagy inhibitor, and U0126, an extracellular signal-related kinases 1 and 2 (ERK1/2) inhibitor, significantly inhibited cell viability, increased intracellular reactive oxygen species levels, promoted cell apoptosis (by upregulating Bax and downregulating Bcl-2), and inhibited cell autophagy (by downregulating Beclin-1 and LC3II/LC3I) in OGD/R-induced H9c2 cells with TGF-β1 overexpression. Additionally, OGD/R induction significantly increased the levels of p-ERK, p-P38, and p-JNK, which were further enhanced by TGF-β1 overexpression. U0126 treatments significantly downregulated the p-ERK compared to OGD/R-induced H9c2 cells with TGF-β1 overexpression. Our study suggests that TGF-β1 could inhibit the growth of cardiomyocytes H9c2 by regulating autophagy and ERK pathways, providing a new theoretical basis for the treatment and prevention of OGD/R in clinical practice.","PeriodicalId":12514,"journal":{"name":"General physiology and biophysics","volume":"43 6","pages":"525-534"},"PeriodicalIF":1.3,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142709821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Neuroprotective effects of coffee-derived exosome-like nanoparticles against Aβ-induced neurotoxicity. 咖啡外泌体纳米颗粒对Aβ诱导的神经毒性的神经保护作用

IF 1.3 4区生物学

General physiology and biophysics Pub Date : 2024-11-01 DOI: 10.4149/gpb_2024025

Meric A Esmekaya, Burhan Ertekin

{"title":"Neuroprotective effects of coffee-derived exosome-like nanoparticles against Aβ-induced neurotoxicity.","authors":"Meric A Esmekaya, Burhan Ertekin","doi":"10.4149/gpb_2024025","DOIUrl":"https://doi.org/10.4149/gpb_2024025","url":null,"abstract":"The present study aimed to provide experimental evidence that CDELNs (coffee-derived exosome-like nanoparticles) may be a candidate for the treatment or prevention of amyloid-β (Aβ)-induced Alzheimer's disease (AD). An in vitro Alzheimer's model was created with Aβ-induced toxicity in mouse hippocampal neuronal cells (HT-22). Aβ(1-42)-exposed cells were treated with different concentrations of CDELNs (1-50 μg/ml) and the viability of cells was analyzed. The change in the mitochondrial membrane potential (ΔΨm) of cells was also determined. CDELNs treatment increased the viability of Aβ(1-42 )-toxicity-induced HT-22 cells significantly. The increase in the viability of Aβ(1-42)-toxicity-induced cells was correlated with an improvement in ΔΨm. CDELNs treatment restored the dissipated ΔΨm. These results suggested that CDELNs protect neuronal cells against Aβ(1-42)-induced neurotoxicity by repairing mitochondrial dysfunction. CDELNs might be a useful neuroprotective agent for the treatment or prevention of Aβ-induced AD. Further animal and clinical studies should be carried out to investigate the neuroprotective potential of CDELNs against Aβ-induced AD.","PeriodicalId":12514,"journal":{"name":"General physiology and biophysics","volume":"43 6","pages":"535-543"},"PeriodicalIF":1.3,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142709816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Extremely low frequency pulsed magnetic field inhibits myocardial damage and apoptosis in rats with CLP-induced sepsis: A histopathological and immunohistochemical evaluation. 极低频脉冲磁场可抑制 CLP 诱导败血症大鼠的心肌损伤和细胞凋亡：组织病理学和免疫组织化学评估。

IF 1.3 4区生物学

General physiology and biophysics Pub Date : 2024-11-01 DOI: 10.4149/gpb_2024029

Serkan Gürgül, Fikret Gevrek, Serkan Yelli, Fatma B Şeker, Can Demirel

{"title":"Extremely low frequency pulsed magnetic field inhibits myocardial damage and apoptosis in rats with CLP-induced sepsis: A histopathological and immunohistochemical evaluation.","authors":"Serkan Gürgül, Fikret Gevrek, Serkan Yelli, Fatma B Şeker, Can Demirel","doi":"10.4149/gpb_2024029","DOIUrl":"https://doi.org/10.4149/gpb_2024029","url":null,"abstract":"We aimed to investigate whether pulsed magnetic fields (PMFs) (1 mT) may have preventive effects on myocardial damage and apoptosis in rats with sepsis. Twenty-eight adult Wistar albino rats were evenly distributed among four experimental groups, each consisting of seven rats: SH, LF-PMF, HF-PMF, and CLP. Sepsis induction was carried out via the cecal ligation and puncture (CLP) method, while rats in the LF-PMF and HF-PMF groups were exposed to 7.5 Hz and 15 Hz PMF, respectively, for duration of 24 hours. Following the removal of heart tissue, histological techniques were employed for the analysis. Histological scoring of apoptosis-related Bax, Bcl-2, and Acas-3 proteins as well as cTnI were performed in the heart tissue. The myocardial damage score significantly increased in the CLP group compared to the SH group (p < 0.05). Significant decreases were observed in Bcl-2 and cTnI protein levels in the CLP group, while significant increases were detected in the PMF groups (p < 0.05). An increase in Bax and Acas-3 protein levels, as well as the Bax/Bcl-2 ratio, was observed in the CLP group, with a decrease in the PMF groups (p < 0.05). The results demonstrate that PMF application has anti-apoptotic and therapeutic effects on septic heart tissue damage.","PeriodicalId":12514,"journal":{"name":"General physiology and biophysics","volume":"43 6","pages":"555-566"},"PeriodicalIF":1.3,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142709813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

HMGB1 impacts the intestinal epithelial barrier by initiating NETs to regulate macrophage polarization. HMGB1 通过启动 NET 来调节巨噬细胞的极化，从而影响肠上皮屏障。

IF 1.3 4区生物学

General physiology and biophysics Pub Date : 2024-11-01 DOI: 10.4149/gpb_2024034

Xiaohong Chen, Junyi Wu, Meng Liu, Zheng Han, Jie Tan, Qingxi Zhu, Xiaodong Huang, Xia Tian

{"title":"HMGB1 impacts the intestinal epithelial barrier by initiating NETs to regulate macrophage polarization.","authors":"Xiaohong Chen, Junyi Wu, Meng Liu, Zheng Han, Jie Tan, Qingxi Zhu, Xiaodong Huang, Xia Tian","doi":"10.4149/gpb_2024034","DOIUrl":"https://doi.org/10.4149/gpb_2024034","url":null,"abstract":"High mobility group box 1 (HMGB1) has the capability of activating the immune response and taking part in macrophage polarization. Despite this, there is significant scope for exploration into how HMGB1 regulates macrophage polarization phenotype and influences intestinal epithelial barrier function. Investigating the role of HMGB1 in the creation of neutrophil extracellular traps (NETs) and the mechanism of its impact on macrophages could provide novel insights into intervening in intestinal inflammation and barrier damage. Therefore, the research examined the relationship between the macrophage polarization phenotype and HMGB1. Additionally, we analyzed how cell proliferation and cytokines changed in CaCo-2 cells following co-culture with HMGB1-influenced macrophages and intestinal epithelial CaCo-2 cells. We discovered that up-regulation of HMGB1 expression enhanced the creation of NETs, whereas inhibition of NETs formation led macrophages to switch from the anti-inflammatory M2 phenotype to the pro-inflammatory M1 phenotype. Additionally, we observed that macrophages induced by NETs containing HMGB1 can prompt CaCo-2 cell apoptosis and exacerbate the inflammatory response. HMGB1-containing NETs hinder tight junction protein expression in CaCo-2 cells by inducing macrophage M1 polarization, thereby impairing intestinal epithelial barrier function. Therefore, our findings indicate that by inhibiting the expression of HMGB1, the formation of NETs can be inhibited. This, in turn, mediates macrophage polarization and offers potential new therapies for intestinal diseases.","PeriodicalId":12514,"journal":{"name":"General physiology and biophysics","volume":"43 6","pages":"545-554"},"PeriodicalIF":1.3,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142709814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Down-regulated miR-10a protects against spinal cord injury by up-regulating SIRT1 COVID-19 与口面裂隙之间的关系。

IF 1.3 4区生物学

General physiology and biophysics Pub Date : 2024-09-01 DOI: 10.4149/gpb_2024024

Chao Song, Yan Zhang

{"title":"Down-regulated miR-10a protects against spinal cord injury by up-regulating SIRT1","authors":"Chao Song, Yan Zhang","doi":"10.4149/gpb_2024024","DOIUrl":"10.4149/gpb_2024024","url":null,"abstract":"MicroRNAs (miRNAs) are essential modulators of gene expression and are associated with various pathological processes, including spinal cord injury (SCI). This investigation aimed to elucidate miR-10a activity in SCI and its potential interaction with sirtuin 1 (SIRT1). The SCI rat model was established to assess hind limb movement, measure levels of miR-10a, SIRT1, neuronal survival, and inflammatory factors. An in-vitro SCI cell model was also developed to evaluate cell viability and inflammatory factor levels. The interaction between miR10a and SIRT1 was verified. Upregulated miR-10a and downregulated SIRT1 expression were found in the tissues of SCI rats. miR-10a knockdown in SCI rats enhanced the recovery of motor function, increased neuronal survival, and reduced the levels of inflammatory cytokines. Luciferase reporter assays confirmed that miR-10a targeted SIRT1 directly. In PC12 cells, downregulation of miR-10a increased SIRT1 expression, enhanced cell viability, and reduced inflammatory factor levels after LPS stimulation. Conversely, SIRT1 knockdown inhibited the protective effects of downregulated miR-10a on cell viability and inflammatory responses. The results suggest that miR-10a downregulation protects against SCI by upregulating SIRT1 expression, improving functional recovery, and reducing inflammation. Targeting the miR-10a/SIRT1 axis is a promising strategy for SCI treatment.","PeriodicalId":12514,"journal":{"name":"General physiology and biophysics","volume":"43 5","pages":"435-443"},"PeriodicalIF":1.3,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141975472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differential expression profiles and functional prediction of circular RNAs in lung cancer patients with chronic obstructive pulmonary disease: a pilot study. 慢性阻塞性肺病肺癌患者循环 RNA 的差异表达谱和功能预测：一项试点研究。

IF 1.3 4区生物学

General physiology and biophysics Pub Date : 2024-07-01 DOI: 10.4149/gpb_2024013

Xiaoou Li, Yongchun Shen, Jiahan Cheng, Jun Chen, Zhicheng Yuan, Tao Wang, Lei Chen, Lunxu Liu, Fuqiang Wen

{"title":"Differential expression profiles and functional prediction of circular RNAs in lung cancer patients with chronic obstructive pulmonary disease: a pilot study.","authors":"Xiaoou Li, Yongchun Shen, Jiahan Cheng, Jun Chen, Zhicheng Yuan, Tao Wang, Lei Chen, Lunxu Liu, Fuqiang Wen","doi":"10.4149/gpb_2024013","DOIUrl":"https://doi.org/10.4149/gpb_2024013","url":null,"abstract":"Chronic obstructive pulmonary disease (COPD), characterized by clinical sub-phenotypes such as emphysema (E) and chronic bronchitis (CB), is associated with a greater risk of lung cancer (LC). This study aimed to assess the expression patterns of circRNA and their potential functional involvement in LC patients with COPD. A circRNA microarray was used to characterize differentially expressed circRNAs (DEcircRNAs) profiles. A total of 176, 240, 163, and 243 DEcircRNAs were identified in comparisons between CB vs. LC patients (Con), E vs. Con, E vs. CB, and CBE vs. Con, respectively. DEcircRNAs in all comparison groups were primarily associated with immune-related GO terms and were also enriched in immune and inflammatory pathways. In total, 49 DEcircRNAs were significantly correlated with the infiltration of multiple immune cells. Among them, hsa-MROH9_0001 and hsa-RP11-35J10_0013 were positively and negatively correlated with plasma cells and T-cell CD4 memory resting cells, respectively; these two DEcircRNA-sponged miRNAs have good diagnostic performance. WGCNA identified six key circRNAs associated with CB progression. The expression patterns of hsa-MROH9_0001 and circRNA_21729 in E and CB groups were confirmed by RT-qPCR. In conclusion, we reported circRNA profiles and the findings demonstrated that hsa-MROH9_0001 and circRNA_21729 may be potential therapeutic targets for LC with COPD.","PeriodicalId":12514,"journal":{"name":"General physiology and biophysics","volume":"43 4","pages":"273-289"},"PeriodicalIF":1.3,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141491577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0