General physiology and biophysics最新文献_第2页

Bombesin stimulates dorsal raphe nucleus serotonergic neurons via a mechanism involving BB1 receptors. Bombesin通过涉及BB1受体的机制刺激中缝背核血清素能神经元。

IF 1.3 4区生物学

General physiology and biophysics Pub Date : 2025-05-01 Epub Date: 2025-03-21 DOI: 10.4149/gpb_2025007

Eliyahu Dremencov, Daniil Grinchii, Xia Zhang, Zul Merali

引用次数: 0

Hsa_circ_0007905 as a modulator of miR-330-5p and VDAC1: Enhancing stemness and reducing apoptosis in cervical cancer stem cells. Hsa_circ_0007905作为miR-330-5p和VDAC1的调节剂：增强宫颈癌干细胞的干性并减少细胞凋亡

IF 1.3 4区生物学

General physiology and biophysics Pub Date : 2025-05-01 DOI: 10.4149/gpb_2024049

JianPing Zheng, Yan Feng, ChaoYan Yuan

{"title":"Hsa_circ_0007905 as a modulator of miR-330-5p and VDAC1: Enhancing stemness and reducing apoptosis in cervical cancer stem cells.","authors":"JianPing Zheng, Yan Feng, ChaoYan Yuan","doi":"10.4149/gpb_2024049","DOIUrl":"https://doi.org/10.4149/gpb_2024049","url":null,"abstract":"Circular RNAs (circRNAs) are covalently closed RNA structures that play a pivotal role in the initiation and progression of cervical cancer (CC). However, it is unclear how these RNAs influence cancer stem cell (CSC)-like properties in CC. Here, we performed circRNA microarray analysis and identified an intergenic circRNA, hsa_circ_0007905, that was significantly upregulated in patients with CC. Moreover, hsa_circ_0007905 was found to be highly expressed in CSC-enriched subsets of cervical cancer cell lines. Functionally, knocking down hsa_circ_0007905 suppressed proliferative, migratory invasive and self-renewal abilities, as well as stimulated apoptosis of CSCs in CC. Mechanistically, hsa_circ_0007905 functions as a \"sponge\" to inversely control miR-330-5p expression, which directly targets VDAC1. Overexpressing VDAC1 or inhibiting miR-330-5p blocked the roles of silencing hsa_circ_0007905 on CSCs. Thus, we revealed the mechanism by which hsa_circ_0007905 competitively adsorbs miR-330-5p to mediate VDAC1 expression to promote stemness and inhibit apoptosis of CSCs in CC, offering an therapeutic target for treating CC.","PeriodicalId":12514,"journal":{"name":"General physiology and biophysics","volume":"44 3","pages":"213-226"},"PeriodicalIF":1.3,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143997070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Crafting extract of Meniran-derived carbon decorated on zinc oxide as composite material for effective cancer treatment. 制作以氧化锌为复合材料装饰的梅尼兰衍生碳提取物，有效治疗癌症。

IF 1.3 4区生物学

General physiology and biophysics Pub Date : 2025-05-01 DOI: 10.4149/gpb_2025003

Mohammad Budiyanto, Fasih B Ilhami, Andika P Wardana, Nora Ertanti, Sapti Puspitarini

引用次数: 0

The expression of circSATB2, PEAK1 in non-small cell lung cancer tissue and their relationships with clinical pathological characteristics, as well as postoperative recurrence and metastasis. circSATB2、PEAK1在非小细胞肺癌组织中的表达及其与临床病理特征及术后复发转移的关系

IF 1.3 4区生物学

General physiology and biophysics Pub Date : 2025-05-01 DOI: 10.4149/gpb_2025005

Hui Li, Jiayun Shan, Longjian Huang

{"title":"The expression of circSATB2, PEAK1 in non-small cell lung cancer tissue and their relationships with clinical pathological characteristics, as well as postoperative recurrence and metastasis.","authors":"Hui Li, Jiayun Shan, Longjian Huang","doi":"10.4149/gpb_2025005","DOIUrl":"https://doi.org/10.4149/gpb_2025005","url":null,"abstract":"We studied the circSATB2 and PEAK1 expression in NSCLC tissues and their associations with clinicopathological features, recurrence, and metastasis. Tumor and adjacent tissues from 98 NSCLC patients (2019-2020) were analyzed using qPCR (circSATB2) and immunohistochemistry (PEAK1). Pearson correlation assessed circSATB2-PEAK1 mRNA relationships. Survival analysis compared high/low expression groups. Cox regression identified risk factors for recurrence/metastasis. circSATB2 and PEAK1 mRNA were upregulated in NSCLC tissues, correlating with lymph node metastasis and advanced TNM stage (IIIa). circSATB2 positively correlated with PEAK1 mRNA. High expression of both predicted poorer survival (Kaplan-Meier). Multivariate analysis identified high circSATB2, high PEAK1, lymph node metastasis, and stage IIIA as independent risk factors for postoperative recurrence/metastasis. In conclusion, elevated circSATB2 and PEAK1 in NSCLC are linked to aggressive clinicopathological features and poor prognosis, serving as biomarkers for recurrence/metastasis risk.","PeriodicalId":12514,"journal":{"name":"General physiology and biophysics","volume":"44 3","pages":"245-251"},"PeriodicalIF":1.3,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144006287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chloroquine induces endothelium-dependent nitric oxide-mediated vasodilation in isolated rat aorta. 氯喹诱导离体大鼠主动脉内皮依赖性一氧化氮介导的血管舒张。

IF 1.3 4区生物学

General physiology and biophysics Pub Date : 2025-05-01 DOI: 10.4149/gpb_2025006

Soo Hee Lee, Kyeong-Eon Park, Yeran Hwang, Sung Il Bae, Seong-Ho Ok, Seung Hyun Ahn, Gyujin Sim, Seunghyeon Park, Ju-Tae Sohn

{"title":"Chloroquine induces endothelium-dependent nitric oxide-mediated vasodilation in isolated rat aorta.","authors":"Soo Hee Lee, Kyeong-Eon Park, Yeran Hwang, Sung Il Bae, Seong-Ho Ok, Seung Hyun Ahn, Gyujin Sim, Seunghyeon Park, Ju-Tae Sohn","doi":"10.4149/gpb_2025006","DOIUrl":"https://doi.org/10.4149/gpb_2025006","url":null,"abstract":"Toxic doses of chloroquine induce vasodilation, which contributes to hypotension. In this study, we aimed to investigate the involvement of endothelial nitric oxide in the vasodilatory effect of chloroquine on isolated rat aortas and elucidate the underlying mechanisms. We evaluated the effects of endothelial denudation and several inhibitors, including the nitric oxide synthase inhibitor NW-nitro-L-arginine methyl ester (L-NAME), the non-specific guanylate cyclase (GC) inhibitor methylene blue, the nitric oxide-sensitive GC inhibitor 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ), and the phosphoinositide-3 kinase inhibitor wortmannin, on chloroquine-induced vasodilation in endothelium-intact aortas. The effects of chloroquine and protein phosphatase 2 (PP2) on the phosphorylation of endothelial nitric oxide synthase (eNOS), Src kinase, and caveolin-1 in human umbilical vein endothelial cells were also investigated. Chloroquine-induced vasodilation was more pronounced in endothelium-intact aortas than in endothelium-denuded ones. L-NAME, methylene blue, ODQ, and wortmannin attenuated the vasodilatory effect of chloroquine in endothelium-intact aortas. Chloroquine increased cyclic guanosine monophosphate (cGMP) levels and stimulatory eNOS phosphorylation (Ser1177) while decreasing inhibitory eNOS phosphorylation (Thr495). PP2 inhibited chloroquine-induced phosphorylation of caveolin-1 and Src kinases. These findings suggest that chloroquine-induced vasodilation is mediated by the eNOS-GC-cGMP pathway, with eNOS phosphorylation regulated by caveolin-1 and Src kinase. Methylene blue may alleviate toxic-dose chloroquine-induced vasodilation.","PeriodicalId":12514,"journal":{"name":"General physiology and biophysics","volume":"44 3","pages":"201-212"},"PeriodicalIF":1.3,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144000891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Myricetin protects airway epithelial cells against cigarette smoke extract-induced inflammation and oxidative stress by suppressing autophagy. 杨梅素通过抑制自噬来保护气道上皮细胞免受香烟烟雾提取物诱导的炎症和氧化应激。

IF 1.3 4区生物学

General physiology and biophysics Pub Date : 2025-03-01 DOI: 10.4149/gpb_2025002

Mi Zhang, Xinyu Song, Ming Zhan

{"title":"Myricetin protects airway epithelial cells against cigarette smoke extract-induced inflammation and oxidative stress by suppressing autophagy.","authors":"Mi Zhang, Xinyu Song, Ming Zhan","doi":"10.4149/gpb_2025002","DOIUrl":"10.4149/gpb_2025002","url":null,"abstract":"Chronic obstructive pulmonary disease (COPD) is a common respiratory disease characterized by inflammation and oxidative stress, which is mainly caused by cigarette smoke (CS). The flavonoid myricetin was reported to exert protective effects in different diseases. This study aimed to explore the function of myricetin in COPD progression. Airway epithelial A549 cells were treated with CS extract (CSE) to establish an in vitro model, followed by the detection of inflammation, oxidative stress, and autophagy markers. Sprague-Dawley male rats were exposed to CS for 12 weeks to establish an in vivo model, followed by the evaluation of lung function parameters and lung histopathological changes. We found that myricetin relieved inflammation and oxidative stress in CSE-induced A549 cells, as demonstrated by the reduced MCP-1, IL-6, and IL-8 expression, ROS production, and MDA content and elevated SOD activity. Myricetin treatment reduced LC3B-II/LC3B-I ratio and Beclin-1 protein levels and elevated p62 protein level after CSE stimulation in A549 cells. In vivo results revealed that myricetin restored pulmonary function and ameliorated pulmonary inflammation and emphysema in CS-induced COPD rats. Collectively, the anti-inflammatory and antioxidant effects of myricetin in COPD may be attributed to its suppressive effects on autophagy.","PeriodicalId":12514,"journal":{"name":"General physiology and biophysics","volume":"44 2","pages":"139-149"},"PeriodicalIF":1.3,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143673800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Co-delivery of circCDR1 and temozolomide with hyaluronic acid-chitosan nanoparticles inhibits glioma progression. circCDR1和替莫唑胺与透明质酸-壳聚糖纳米颗粒共同递送可抑制胶质瘤进展。

IF 1.3 4区生物学

General physiology and biophysics Pub Date : 2025-03-01 DOI: 10.4149/gpb_2024048

Changjiu Tang, Ye Wan, Xiaomin Zhang, Bao Zhong, Ming Zhang

引用次数: 0

LncRNA ROR promotes proliferation, immune escape, and polarization of M2 macrophages in thyroid cancer by activating the PI3K/AKT pathway. LncRNA ROR通过激活PI3K/AKT通路促进甲状腺癌中M2巨噬细胞的增殖、免疫逃逸和极化。

IF 1.3 4区生物学

General physiology and biophysics Pub Date : 2025-03-01 DOI: 10.4149/gpb_2025001

Xueli Hu, Rong Tan, Nannan Li, Jing Liu, Senli Wen, Haipeng Tang, Yaling Jiang

{"title":"LncRNA ROR promotes proliferation, immune escape, and polarization of M2 macrophages in thyroid cancer by activating the PI3K/AKT pathway.","authors":"Xueli Hu, Rong Tan, Nannan Li, Jing Liu, Senli Wen, Haipeng Tang, Yaling Jiang","doi":"10.4149/gpb_2025001","DOIUrl":"10.4149/gpb_2025001","url":null,"abstract":"Thyroid cancer is the most prominent type of endocrine cancer. LncRNA ROR (Linc- ROR) exerts tumor regulator function in cancers. This study elucidates the action of Linc-ROR on thyroid cancer. The Linc-ROR level were investigated in 70 thyroid cancer patients. Cell viability was detected utilizing CCK-8 method. The xenograft tumor was constructed to evaluate tumor growth. The proportion of CD8+ T cells was assessed using flow cytometry. IL-10, IFN-γ, and TNF-β levels were detected utilizing ELISA assays. The Linc-ROR level was elevated in thyroid cancer patients (n = 70). The up-regulated Linc-ROR was associated with poor overall survival (p = 0.0315), lymph node metastasis (p = 0.027), and TNM stage (p = 0.016). Linc-ROR silencing restrained cell proliferation and tumor growth (p < 0.01). Additionally, silenced Linc-ROR suppressed the immune escape of thyroid cancer cells and polarization of M2 macrophages (p < 0.01). Moreover, the PI3K/ AKT signaling mediated the action of silenced Linc-ROR on thyroid cancer proliferation, immune escape, and M2 macrophage polarization (p < 0.05). Linc-ROR may be a valuable target for thyroid cancer management. The limitations of this research are that the action of Linc-ROR on the tumor microenvironment has not been investigated in the animal model.","PeriodicalId":12514,"journal":{"name":"General physiology and biophysics","volume":"44 2","pages":"163-173"},"PeriodicalIF":1.3,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143673747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to: Determination of selected dynamic quantities of growing intact seeds of maize. 修正：玉米完整种子生长的选定动态数量的测定。

IF 1.3 4区生物学

General physiology and biophysics Pub Date : 2025-03-01 DOI: 10.4149/gpb_2025004

Monika Olszewska, Aleksandra Haduch-Sendecka, Mariusz Pietruszka

引用次数: 0

miR-145-5p modulates CDCA3 to overcome gemcitabine resistance in pancreatic cancer. miR-145-5p调节CDCA3以克服胰腺癌中的吉西他滨耐药。

IF 1.3 4区生物学

General physiology and biophysics Pub Date : 2025-03-01 DOI: 10.4149/gpb_2024047

Jiayun Ge, Zhihong Zhang, Guohui Shao, Dong Wei, Bo Tang, Zhitian Shi, Renchao Zou, Xin Wang

引用次数: 0