General physiology and biophysics最新文献

Bombesin stimulates dorsal raphe nucleus serotonergic neurons via a mechanism involving BB1 receptors.

IF 1.3 4区生物学

General physiology and biophysics Pub Date : 2025-03-21 DOI: 10.4149/gpb_2025007

Eliyahu Dremencov, Daniil Grinchii, Xia Zhang, Zul Merali

引用次数: 0

Myricetin protects airway epithelial cells against cigarette smoke extract-induced inflammation and oxidative stress by suppressing autophagy.

IF 1.3 4区生物学

General physiology and biophysics Pub Date : 2025-03-01 DOI: 10.4149/gpb_2025002

Mi Zhang, Xinyu Song, Ming Zhan

{"title":"Myricetin protects airway epithelial cells against cigarette smoke extract-induced inflammation and oxidative stress by suppressing autophagy.","authors":"Mi Zhang, Xinyu Song, Ming Zhan","doi":"10.4149/gpb_2025002","DOIUrl":"https://doi.org/10.4149/gpb_2025002","url":null,"abstract":"Chronic obstructive pulmonary disease (COPD) is a common respiratory disease characterized by inflammation and oxidative stress, which is mainly caused by cigarette smoke (CS). The flavonoid myricetin was reported to exert protective effects in different diseases. This study aimed to explore the function of myricetin in COPD progression. Airway epithelial A549 cells were treated with CS extract (CSE) to establish an in vitro model, followed by the detection of inflammation, oxidative stress, and autophagy markers. Sprague-Dawley male rats were exposed to CS for 12 weeks to establish an in vivo model, followed by the evaluation of lung function parameters and lung histopathological changes. We found that myricetin relieved inflammation and oxidative stress in CSE-induced A549 cells, as demonstrated by the reduced MCP-1, IL-6, and IL-8 expression, ROS production, and MDA content and elevated SOD activity. Myricetin treatment reduced LC3B-II/LC3B-I ratio and Beclin-1 protein levels and elevated p62 protein level after CSE stimulation in A549 cells. In vivo results revealed that myricetin restored pulmonary function and ameliorated pulmonary inflammation and emphysema in CS-induced COPD rats. Collectively, the anti-inflammatory and antioxidant effects of myricetin in COPD may be attributed to its suppressive effects on autophagy.","PeriodicalId":12514,"journal":{"name":"General physiology and biophysics","volume":"44 2","pages":"139-149"},"PeriodicalIF":1.3,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143673800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Co-delivery of circCDR1 and temozolomide with hyaluronic acid-chitosan nanoparticles inhibits glioma progression.

IF 1.3 4区生物学

General physiology and biophysics Pub Date : 2025-03-01 DOI: 10.4149/gpb_2024048

Changjiu Tang, Ye Wan, Xiaomin Zhang, Bao Zhong, Ming Zhang

引用次数: 0

LncRNA ROR promotes proliferation, immune escape, and polarization of M2 macrophages in thyroid cancer by activating the PI3K/AKT pathway.

IF 1.3 4区生物学

General physiology and biophysics Pub Date : 2025-03-01 DOI: 10.4149/gpb_2025001

Xueli Hu, Rong Tan, Nannan Li, Jing Liu, Senli Wen, Haipeng Tang, Yaling Jiang

{"title":"LncRNA ROR promotes proliferation, immune escape, and polarization of M2 macrophages in thyroid cancer by activating the PI3K/AKT pathway.","authors":"Xueli Hu, Rong Tan, Nannan Li, Jing Liu, Senli Wen, Haipeng Tang, Yaling Jiang","doi":"10.4149/gpb_2025001","DOIUrl":"https://doi.org/10.4149/gpb_2025001","url":null,"abstract":"Thyroid cancer is the most prominent type of endocrine cancer. LncRNA ROR (Linc- ROR) exerts tumor regulator function in cancers. This study elucidates the action of Linc-ROR on thyroid cancer. The Linc-ROR level were investigated in 70 thyroid cancer patients. Cell viability was detected utilizing CCK-8 method. The xenograft tumor was constructed to evaluate tumor growth. The proportion of CD8+ T cells was assessed using flow cytometry. IL-10, IFN-γ, and TNF-β levels were detected utilizing ELISA assays. The Linc-ROR level was elevated in thyroid cancer patients (n = 70). The up-regulated Linc-ROR was associated with poor overall survival (p = 0.0315), lymph node metastasis (p = 0.027), and TNM stage (p = 0.016). Linc-ROR silencing restrained cell proliferation and tumor growth (p < 0.01). Additionally, silenced Linc-ROR suppressed the immune escape of thyroid cancer cells and polarization of M2 macrophages (p < 0.01). Moreover, the PI3K/ AKT signaling mediated the action of silenced Linc-ROR on thyroid cancer proliferation, immune escape, and M2 macrophage polarization (p < 0.05). Linc-ROR may be a valuable target for thyroid cancer management. The limitations of this research are that the action of Linc-ROR on the tumor microenvironment has not been investigated in the animal model.","PeriodicalId":12514,"journal":{"name":"General physiology and biophysics","volume":"44 2","pages":"163-173"},"PeriodicalIF":1.3,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143673747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to: Determination of selected dynamic quantities of growing intact seeds of maize.

IF 1.3 4区生物学

General physiology and biophysics Pub Date : 2025-03-01 DOI: 10.4149/gpb_2025004

Monika Olszewska, Aleksandra Haduch-Sendecka, Mariusz Pietruszka

引用次数: 0

miR-145-5p modulates CDCA3 to overcome gemcitabine resistance in pancreatic cancer.

IF 1.3 4区生物学

General physiology and biophysics Pub Date : 2025-03-01 DOI: 10.4149/gpb_2024047

Jiayun Ge, Zhihong Zhang, Guohui Shao, Dong Wei, Bo Tang, Zhitian Shi, Renchao Zou, Xin Wang

引用次数: 0

Synthesis of Epimedium extract selenium nanoparticles and evaluation their efficacy against lung cancer.

IF 1.3 4区生物学

General physiology and biophysics Pub Date : 2025-03-01 DOI: 10.4149/gpb_2024046

Guangying Fu, Jin Tong

{"title":"Synthesis of Epimedium extract selenium nanoparticles and evaluation their efficacy against lung cancer.","authors":"Guangying Fu, Jin Tong","doi":"10.4149/gpb_2024046","DOIUrl":"https://doi.org/10.4149/gpb_2024046","url":null,"abstract":"Lung cancer, the foremost cause of cancer-related mortality worldwide, necessitates the exploration for novel anti-lung cancer therapeutics to enhance efficacy and reduce adverse effects. Targeting selenium in the tumor microenvironment has become a new strategy for the treatment of lung cancer. The objective of this study was to synthesize novel selenium nanoparticles (EBM-SeNPs) using aqueous extracts derived from Epimedium brevicornum Maxim and investigate its structural characteristics and inhibitory effects against lung cancer. The physicochemical properties of EBM-SeNPs were characterized from multiple aspects using a variety of methods. The CCK-8, flow cytometry, wound healing assay, Western blot and the cell derived xenograft model were conducted to evaluate the antitumor efficacy in vivo and in vitro. EBM-SeNPs were approximately spherical and exhibited superior dispersivity and stability in water solution. And EBM-SeNPs did not display any specific cytotoxicity against human liver cells. However, they showed outstanding capability to induce apoptosis in lung cancer cells, thereby effectively suppressing their growth and migratory potential. Furthermore, EBM-SeNPs demonstrated a reduction of tumor and an increase in immune organ index of tumor-bearing mice. Collectively, the EBM-SeNPs may be an effective and safe option for the treatment of lung cancer.","PeriodicalId":12514,"journal":{"name":"General physiology and biophysics","volume":"44 2","pages":"123-138"},"PeriodicalIF":1.3,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143673802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effects of stress and environmental enrichment on cognitive functions and stress-related gene expressions in the brain of aged rats. 压力和丰富环境对老年大鼠大脑认知功能和压力相关基因表达的影响

IF 1.3 4区生物学

General physiology and biophysics Pub Date : 2025-03-01 DOI: 10.4149/gpb_2024044

Duygu S Oran, Evren Eraslan

{"title":"The effects of stress and environmental enrichment on cognitive functions and stress-related gene expressions in the brain of aged rats.","authors":"Duygu S Oran, Evren Eraslan","doi":"10.4149/gpb_2024044","DOIUrl":"https://doi.org/10.4149/gpb_2024044","url":null,"abstract":"We aimed to investigate whether environmental enrichment (EE) would alter possible adverse effects of chronic unpredictable mild stress (CUMS) in elderly rats regarding corticosterone levels, stress-related gene expressions in some brain regions, and learning and memory. Wistar male rats (over 20 months) weighing 450-550 g were housed in enriched or standard cages for the duration of the study (10 weeks). After 8 weeks of CUMS application, body weight gain, adrenal weight, and corticosterone levels were measured. Morris water maze (MWM), and novel object recognition test were performed. Glucocorticoid receptor (GR), corticotropin-releasing hormone (CRH), and corticotropin-releasing hormone receptor 1 (CRHR1) expression levels were determined in the hypothalamus and hippocampus. In the stress group, body weights decreased over time. Regarding the distance swum by rats to find the platform in the MWM, while there was no significant difference between the 3rd and 4th days in the EE+CUMS group, the decrease continued until the 4th day in the standard control (SC)+CUMS group. Stress application reduced the GR and CRHR1 gene expressions in the hypothalamus. We conclude that chronic stress and EE caused brain region-specific changes, thus affecting the neurobiological and cognitive functions in the elderly. In this respect, our study will contribute to neurobiological and neurodegenerative studies on aging.","PeriodicalId":12514,"journal":{"name":"General physiology and biophysics","volume":"44 2","pages":"151-162"},"PeriodicalIF":1.3,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143673805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

GZMA silencing inhibits JAK2/STAT1 pathway and improves allergic rhinitis. 抑制 GZMA 可抑制 JAK2/STAT1 通路并改善过敏性鼻炎。

IF 1.3 4区生物学

General physiology and biophysics Pub Date : 2025-03-01 DOI: 10.4149/gpb_2024045

Lin Li, Yuhao Zhang, Jingyuan Wang, Lixia Zhang, Ying Gao, Xiaolin Ji, Tian Wang, Fei Zhao

{"title":"GZMA silencing inhibits JAK2/STAT1 pathway and improves allergic rhinitis.","authors":"Lin Li, Yuhao Zhang, Jingyuan Wang, Lixia Zhang, Ying Gao, Xiaolin Ji, Tian Wang, Fei Zhao","doi":"10.4149/gpb_2024045","DOIUrl":"https://doi.org/10.4149/gpb_2024045","url":null,"abstract":"Allergic rhinitis (AR) is an immunoglobulin E (IgE)-mediated inflammatory disorder. This study attempts to identify AR-related differential expressed genes (DEGs) and determine potential targets for AR. We employed bioinformatics analysis to screen for hub DEGs for AR, and their performances in distinguishing AR were assessed by receiver operating characteristic (ROC) curves. Quantitative reverse-transcription polymerase chain reaction (qRT-PCR) and Western blot was used to quantify Granzyme A (GZMA) in ovalbumin (OVA)-induced AR mice. TNF-α-induced cell model was utilized to assess the role of GZMA in AR, and the effect of GZMA silencing on the JAK2/STAT1 pathway was investigated in TNF-α-induced AR. We identified HIST1H2BD, RPS28, HIST1H1C, MAF, and GZMA as hub genes, all of which exhibited excellent performance in distinguishing between AR and controls (AUC > 0.800). GZMA was highly expressed in AR mice. Silencing GZMA reduced the levels of inflammatory cytokines (IL-6, IL-4 and IL-5), inhibited cell apoptosis and promoted cell proliferation in TNF-α-induced nasal mucosal epithelial cells (MIC-iCell-m024). Overexpression of GZMA exhibited the opposite effects by promoting inflammation and cell apoptosis but inhibiting proliferation. Mechanistically, silencing GZMA inhibited the phosphorylation of JAK2 and STAT1, indicating the suppression of JAK2/STAT1 pathway. This study might share new idea for AR management.","PeriodicalId":12514,"journal":{"name":"General physiology and biophysics","volume":"44 2","pages":"93-106"},"PeriodicalIF":1.3,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143673741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pulmonary alveolar proteinosis: Clinical and morphological overview of a rare disease associated with macrophage dysfunction. 肺泡蛋白沉积症：一种与巨噬细胞功能障碍相关的罕见疾病的临床和形态学综述。

IF 1.3 4区生物学

General physiology and biophysics Pub Date : 2025-01-01 DOI: 10.4149/gpb_2024038

Brigita Javorská, Róbert Slivka, Barbora Durcová, Adela Vrbenská, Jozef Škarda, Janka Vecanová, Natália Hvizdošová, Mária Makovická, Vojtěch Kamarád, Jozef Muri

{"title":"Pulmonary alveolar proteinosis: Clinical and morphological overview of a rare disease associated with macrophage dysfunction.","authors":"Brigita Javorská, Róbert Slivka, Barbora Durcová, Adela Vrbenská, Jozef Škarda, Janka Vecanová, Natália Hvizdošová, Mária Makovická, Vojtěch Kamarád, Jozef Muri","doi":"10.4149/gpb_2024038","DOIUrl":"https://doi.org/10.4149/gpb_2024038","url":null,"abstract":"Pulmonary alveolar proteinosis (PAP) is a rare disease characterised by excessive accumulation of surfactant components in alveolar macrophages, alveoli, and peripheral airways. The accumulation of surfactant is associated with only a minimal inflammatory response but can lead to the development of pulmonary fibrosis. Three clinical forms of PAP are distinguished - primary, secondary and congenital. In recent years, significant findings have helped to clarify the ethiology and pathogenesis of the disease. Apart from impaired surfactant protein function, a key role in the development of PAP is played by signal pathway of granulocyte and macrophage colonies stimulating growth factor (GM-CSF) which is necessary for the functioning of alveolar macrophages and for surfactant homeostasis. Surfactant is partially degraded by alveolar macrophages that are stimulated by GM-CSF. The role of GM-CSF has been shown especially in primary PAP, which is currently considered an autoimmune disease involving the development of GM-CSF neutralising autoantibodies. Clinically, the disease may be silent or manifest with dyspnoeic symptoms triggered by exertion and cough. However, there is a 10 to 15% rate of patients who develop respiratory failure. Total pulmonary lavage is regarded as the standard method of treatment. In addition, recombinant human GM-CSF has been studied as a prospective therapy for the treatment of PAP.","PeriodicalId":12514,"journal":{"name":"General physiology and biophysics","volume":"44 1","pages":"1-11"},"PeriodicalIF":1.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143003032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0