General physiology and biophysics最新文献

USP7/SETDB1 promotes non-small cell lung cancer progression by regulating ferroptosis via NRF2/SLC7A11/GPX4 axis. USP7/SETDB1通过NRF2/SLC7A11/GPX4轴调控铁凋亡，促进非小细胞肺癌进展。

IF 1.5 4区生物学

General physiology and biophysics Pub Date : 2026-09-01 DOI: 10.4149/gpb_2026011

Hongpei Zhang, Yanfeng Fang, Xinxin Wang, Wenting Wu, Dandan Li, Yuan Zhang

{"title":"USP7/SETDB1 promotes non-small cell lung cancer progression by regulating ferroptosis via NRF2/SLC7A11/GPX4 axis.","authors":"Hongpei Zhang, Yanfeng Fang, Xinxin Wang, Wenting Wu, Dandan Li, Yuan Zhang","doi":"10.4149/gpb_2026011","DOIUrl":"https://doi.org/10.4149/gpb_2026011","url":null,"abstract":"Non-small cell lung cancer (NSCLC) is a malignant tumor with high morbidity and mortality. Given the reported associations of both SET domain bifurcated histone lysine methyltransferase 1 (SETDB1) with various cancers and ubiquitin-specific protease 7 (USP7) with NSCLC progression, this study aims to elucidate the role of SETDB1 and USP7 in NSCLC progression. mRNA and protein levels were measured by qRT-PCR and Western blot, respectively. Cell biological phenotypes were evaluated using MTT, EdU, flow cytometry, transwell, and sphere formation assays. Ferroptosis was analyzed by measuring Fe2+, MDA GSH, and ROS levels. Relationship between USP7 and SETDB1 was examined through Co-IP, cycloheximide (CHX) chase, and deubiquitination assays. Xenograft tumor models were established to investigate the role of SETDB1 and USP7 in NSCLC in vivo. In NSCLC tissues and cells, SETDB1 levels were elevated. Knockdown of SETDB1 inhibited NSCLC cell viability, proliferation, invasion, and sphere formation ability, while promoting apoptosis and ferroptosis. Mechanistically, USP7 enhanced the stability of SETDB1 protein by its deubiquitination. USP7 knockdown suppressed NSCLC cell biological behavior by downregulating SETDB1. Moreover, USP7 knockdown repressed in vivo tumor growth of NSCLC. Taken together, USP7 suppresses ferroptosis and enhances growth in NSCLC cells by stabilizing SETDB1 protein through deubiquitination.","PeriodicalId":12514,"journal":{"name":"General physiology and biophysics","volume":"45 5","pages":"467-480"},"PeriodicalIF":1.5,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148919320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bardoxolone methyl induces mitochondrial dysfunction, activation of the cytosolic stress response, and increased chaperone capacity in SH-SY5Y cells. Focus on mitochondrial protease LONP1. 甲基巴多洛酮诱导SH-SY5Y细胞线粒体功能障碍，激活胞质应激反应，增加伴侣能力。重点研究线粒体蛋白酶LONP1。

IF 1.5 4区生物学

General physiology and biophysics Pub Date : 2026-09-01 DOI: 10.4149/gpb_2026019

Lubos Hudak, Andrea Evinova, Monika Liskova, Jaroslava Guzikova, Michal Pokusa, Zuzana Tatarkova, Maria Brodnanova, Magdalena Kurekova, Patrik Jambrich, Peter Racay

{"title":"Bardoxolone methyl induces mitochondrial dysfunction, activation of the cytosolic stress response, and increased chaperone capacity in SH-SY5Y cells. Focus on mitochondrial protease LONP1.","authors":"Lubos Hudak, Andrea Evinova, Monika Liskova, Jaroslava Guzikova, Michal Pokusa, Zuzana Tatarkova, Maria Brodnanova, Magdalena Kurekova, Patrik Jambrich, Peter Racay","doi":"10.4149/gpb_2026019","DOIUrl":"https://doi.org/10.4149/gpb_2026019","url":null,"abstract":"Our work aimed to study the impact of bardoxolone methyl, 2-cyano-3, 12-dioxooleana-1,9(11)-dien-28-oic acid methyl ester (CDDO-Me), on mitochondrial function and morphology in SH-SY5Y cells, molecular responses, with a focus on the expression of proteins of mitochondriaspecific unfolded protein response (mtUPR), the endoplasmic reticulum-specific UPR (UPRER), and the cytosolic stress response. Treatment of SH-SY5Y cells with CDDO-Me is associated with decreased relative cell survival associated with gasdermin E cleavage, compatible with pyroptotic cell death features. Treatment of SH-SY5Y cells with CDDO-Me results in decreased ROUTINE respiration, maximal respiration, succinate-driven maximal respiration, ATP-coupled respiration, and spare respiratory capacity. We have not observed significant changes in the expression of proteins that play important roles in both mtUPR (LONP1) and UPRER (HRD1 and SEL1L), whereas expression of cytosolic chaperone HSP70 was significantly increased in response to CDDO-Me. In addition, we have observed increased expression of mitochondrial chaperones HSP60 and GRP75 as well as ER chaperone GRP78. Finally, we have observed the formation of donut-like mitochondria induced by CDDO-Me. Our results, together with previously published data, indicate that mitochondrial dysfunction, activation of cytosolic stress response, and an increase in chaperone capacity elicited by CDDO-Me could be attributed to CDDO-Me's ability to inhibit LONP1 protease.","PeriodicalId":12514,"journal":{"name":"General physiology and biophysics","volume":"45 5","pages":"455-466"},"PeriodicalIF":1.5,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148919356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

KIAA1429 promotes chondrocyte senescence and ferroptosis in osteoarthritis progression by facilitating m6A-mediated downregulation of USP3. KIAA1429通过促进m6a介导的USP3下调，促进骨关节炎进展中的软骨细胞衰老和铁下垂。

IF 1.5 4区生物学

General physiology and biophysics Pub Date : 2026-09-01 DOI: 10.4149/gpb_2026020

Binqing Ma, Jiangbo Zhao, Chen Cheng, Biao Yang

{"title":"KIAA1429 promotes chondrocyte senescence and ferroptosis in osteoarthritis progression by facilitating m6A-mediated downregulation of USP3.","authors":"Binqing Ma, Jiangbo Zhao, Chen Cheng, Biao Yang","doi":"10.4149/gpb_2026020","DOIUrl":"https://doi.org/10.4149/gpb_2026020","url":null,"abstract":"Osteoarthritis (OA) is a common orthopedic disorder with complicated pathological mechanisms. This study aimed to uncover a novel regulatory network related to vir-like m6A methyltransferase-associated protein KIAA1429 (also known as VIRMA) and ubiquitin-specific protease 3 (USP3) in the progression of OA. An in vitro model of OA was constructed by treating chondrocytes (C28/I2) with interleukin-1β (IL-1β). qRT-PCR and Western blot were applied for gene and protein expression detection. Cell viability was examined using a CCK-8 assay. Cell death was evaluated by flow cytometry following Annexin V/PI staining. Cell senescence was assessed by senescence-associated β-galactosidase (SA-β-gal) staining. Ferroptosis-related events were detected by commercial kits. The interaction between KIAA1429 and USP3 was confirmed via RNA immunoprecipitation assay and dual-luciferase reporter assay. OA mice model was induced by surgical destabilization of the medial meniscus (DMM). Results showed that KIAA1429 expression was up-regulated in C28/I2 cells after IL-1β exposure. Inhibition of KIAA1429 impeded senescence and ferroptosis in IL-1β-treated C28/I2 cells. Mechanistically, KIAA1429 reduced USP3 mRNA expression through m6A methylation modification. Moreover, the protective effects of KIAA1429 knockdown on IL-1β-induced senescence and ferroptosis in C28/I2 cells were rescued by USP3 downregulation. In vivo, KIAA1429 downregulation suppressed the development of OA in DMM mice. These results elucidate that KIAA1429 promotes the senescence and ferroptosis of chondrocytes by increasing m6A methylation of USP3 to suppress its stability, thereby contributing to the pathogenesis of OA.","PeriodicalId":12514,"journal":{"name":"General physiology and biophysics","volume":"45 5","pages":"441-453"},"PeriodicalIF":1.5,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148919280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Aerobic exercise regulates the cGAS/STING signaling pathway and inhibits ferroptosis to alleviate ischemic stroke. 有氧运动调节cGAS/STING信号通路，抑制铁下垂减轻缺血性脑卒中。

IF 1.5 4区生物学

General physiology and biophysics Pub Date : 2026-09-01 DOI: 10.4149/gpb_2026023

Ying He, Ying Zhao, Chunjun Zhang, Hang Yang, Tengmin Gui, Liqing Yao

{"title":"Aerobic exercise regulates the cGAS/STING signaling pathway and inhibits ferroptosis to alleviate ischemic stroke.","authors":"Ying He, Ying Zhao, Chunjun Zhang, Hang Yang, Tengmin Gui, Liqing Yao","doi":"10.4149/gpb_2026023","DOIUrl":"https://doi.org/10.4149/gpb_2026023","url":null,"abstract":"Aerobic exercise at the appropriate intensity alleviates motor dysfunction after ischemic stroke (IS); however, its regulatory mechanisms remain to be elucidated. In this study, the neuroprotective effects of exercise and the related mechanisms were investigated using a rat middle cerebral artery occlusion (MCAO) model and an HT22/PC12 cell oxygen-glucose deprivation/reperfusion (OGD/R) model. The rats were subjected to daily exercise training for durations of 3, 7 or 14 days post-surgery. The results revealed that exercise, particularly the 14-day regimen, significantly reduced the mNSS and infarct area, restored the number of Nissl bodies, and ameliorated cortical pathology. Exercise also decreased Fe²+, MDA, and IREB2 levels and increased SOD, GPX4, and SLC7A11 levels, indicating the inhibition of ferroptosis. In addition, the ferroptosis inhibitor Fer-1 attenuated OGD/R-induced damage in HT22 and PC12 cells. In terms of molecular mechanisms, we revealed that the cGAS/STING signaling pathway was activated and that knockdown of cGAS or STING restored cell viability and mitochondrial structure by inhibiting ferroptosis and reducing cell damage. Importantly, exercise also alleviated IS progression in rats by downregulating the expression of cGAS and STING. In conclusion, aerobic exercise inhibits ferroptosis via suppression of the cGAS/STING pathway, thereby alleviating IS progression.","PeriodicalId":12514,"journal":{"name":"General physiology and biophysics","volume":"45 5","pages":"425-440"},"PeriodicalIF":1.5,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148919293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcription factor KLF4 suppresses the malignant progression of colorectal cancer by activating ETFDH transcription. 转录因子KLF4通过激活ETFDH转录抑制结直肠癌的恶性进展。

IF 1.5 4区生物学

General physiology and biophysics Pub Date : 2026-09-01 DOI: 10.4149/gpb_2026027

Yan Zeng, Qiaozhen Liu, Yali Xu, Tong Zhao, Zhiping Hu

{"title":"Transcription factor KLF4 suppresses the malignant progression of colorectal cancer by activating ETFDH transcription.","authors":"Yan Zeng, Qiaozhen Liu, Yali Xu, Tong Zhao, Zhiping Hu","doi":"10.4149/gpb_2026027","DOIUrl":"https://doi.org/10.4149/gpb_2026027","url":null,"abstract":"Colorectal cancer (CRC) is a common cause of cancer-related deaths worldwide. Electron transfer flavoprotein dehydrogenase (ETFDH) is a novel prognostic biomarker for human CRC. However, the role and molecular mechanism of ETFDH in CRC are still unclear. DEGs in CRC development were identified using mRNA microarray datasets GSE23011, GSE44076, and GSE113513. Gene expression was determined using RT-qPCR and Western blot. Cell proliferation, migration, and invasion were detected by EdU, Colony formation, Transwell, and wound healing. Effects of CRC cells on CD8+ T cell apoptosis were analyzed using flow cytometry. Effects of ETFDH on CRC cell growth in vivo was analyzed using xenograft model. After JASPAR prediction, the binding between Transcription factor Kruppel-like factor 4 (KLF4) and ETFDH promoter was verified using ChIP and dual-luciferase reporter. ETFDH and KLF4 were decreased in CRC tissues and cells. ETFDH upregulation blocked CRC cell proliferation, migration, invasion, and repress the apoptosis of CD8+ T cells in vitro. ETFDH overexpression hindered CRC tumor growth in vivo. Mechanistically, KLF4 enhanced the transcriptional activity of ETFDH via binding to its promoter region. KLF4-activated ETFDH transcription suppresses CRC cell malignant behavior and decreases the apoptosis of CD8+T cells, which provides a promising therapeutic target for CRC.","PeriodicalId":12514,"journal":{"name":"General physiology and biophysics","volume":"45 5","pages":"481-494"},"PeriodicalIF":1.5,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148919261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electroacupuncture alleviates synovial inflammation in rheumatoid arthritis rats by suppressing caspase-1-mediated pyroptosis. 电针通过抑制caspase-1介导的热凋亡减轻类风湿关节炎大鼠滑膜炎症。

IF 1.5 4区生物学

General physiology and biophysics Pub Date : 2026-09-01 DOI: 10.4149/gpb_2026024

Xiling Chen, Kui Fang, Peng Xu, Xiaojing Nan, Jinbiao You, Yingjun Xiao, Zheqing Dong

{"title":"Electroacupuncture alleviates synovial inflammation in rheumatoid arthritis rats by suppressing caspase-1-mediated pyroptosis.","authors":"Xiling Chen, Kui Fang, Peng Xu, Xiaojing Nan, Jinbiao You, Yingjun Xiao, Zheqing Dong","doi":"10.4149/gpb_2026024","DOIUrl":"https://doi.org/10.4149/gpb_2026024","url":null,"abstract":"Rheumatoid arthritis (RA) causes inflammatory bone and cartilage damage. While electroacupuncture (EA) alleviates joint inflammation, its anti-inflammatory mechanisms remain unclear. Sprague-Dawley rats were randomly assigned to control, collagen-induced arthritis (CIA), EA (EA+CIA), and sham-EA (sham-EA+CIA) groups. Rats in the EA group received EA at unilateral ST36 (Zusanli) every other day from day 1. Paw swelling and paw withdrawal latency (PWL) were measured during the treatment. The levels of caspase-1, GSDMD, IL-1β, and IL-18 in the synovium were assessed after 28 days of EA treatment. Our findings demonstrated that inflammatory responses, such as the arthritis index, toe volume, PWL, and inflammatory cell infiltration, were significantly improved in the EA group compared with the CIA and sham-EA groups. Significant decreases in the levels of caspase-1, GSDMD, IL-1β, and IL-18 were observed in the synovium of RA rats that received EA. Furthermore, peripheral blood levels of the inflammatory cytokines IL-1β and IL-18 were significantly reduced in rats in the EA group. Electroacupuncture as implemented at ST36 was associated with reduced caspase-1-mediated pyroptosis-related markers in the joint synovium and decreased IL-1β and IL-18 levels in both synovium and serum in rats with CIA. Acupoint-specificity requires further validation using active-sham or componentresolved control designs.","PeriodicalId":12514,"journal":{"name":"General physiology and biophysics","volume":"45 5","pages":"519-528"},"PeriodicalIF":1.5,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148919307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cycloartenol attenuates cell motion and angiogenesis by regulating VEGFA/VEGFR2 signaling pathway in gastric cancer. 环artenol通过调节胃癌中VEGFA/VEGFR2信号通路减弱细胞运动和血管生成。

IF 1.5 4区生物学

General physiology and biophysics Pub Date : 2026-09-01 DOI: 10.4149/gpb_2026022

Cheng Chang, Geng Huang, Zheng Fang

{"title":"Cycloartenol attenuates cell motion and angiogenesis by regulating VEGFA/VEGFR2 signaling pathway in gastric cancer.","authors":"Cheng Chang, Geng Huang, Zheng Fang","doi":"10.4149/gpb_2026022","DOIUrl":"https://doi.org/10.4149/gpb_2026022","url":null,"abstract":"Xiaotansanjiefang, a traditional Chinese medicine formula containing pinellia rhizomes, is investigated for anti-gastric cancer (GC) activity. Cycloartenol, a triterpenoid saponin from pinellia, exhibits emerging anti-tumor properties. This study evaluates its effects on GC cell migration, invasion, and angiogenesis. Cytotoxicity of cycloartenol (0-160 μM) was assessed in GC cells (AGS and MKN-45) and immortalized normal gastric epithelial cells (GES-1) via MTT assays. Colony formation, wound healing, and Transwell assays were conducted to assess proliferation, migration, and invasion. Angiogenesis was evaluated by HUVEC tube formation using conditioned media harvested from cycloartenol-treated GC cells. VEGFA and VEGFR2 protein levels were measured by Western blotting. Cycloartenol showed dose-dependent cytotoxicity in GC cells with minimal toxicity toward GES-1 cells, indicating selectivity. It suppressed colony formation, delayed wound closure, reduced invading cells, and inhibited HUVEC tube formation. Consistently, cycloartenol downregulated VEGFA and VEGFR2 expression in GC cells and HUVECs. In conclusion, cycloartenol selectively suppresses GC cell growth, migration, invasion, and angiogenesis through inactivation of the VEGFA/VEGFR2 pathway.","PeriodicalId":12514,"journal":{"name":"General physiology and biophysics","volume":"45 5","pages":"529-537"},"PeriodicalIF":1.5,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148919317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

KRT19 promotes colorectal cancer progression through mTOR pathway via SIRT4. KRT19通过SIRT4通过mTOR途径促进结直肠癌进展。

IF 1.5 4区生物学

General physiology and biophysics Pub Date : 2026-09-01 DOI: 10.4149/gpb_2026026

Zhu Qiran, Cui Ying, Cheng Cheng, Zhang Bomiao

{"title":"KRT19 promotes colorectal cancer progression through mTOR pathway via SIRT4.","authors":"Zhu Qiran, Cui Ying, Cheng Cheng, Zhang Bomiao","doi":"10.4149/gpb_2026026","DOIUrl":"https://doi.org/10.4149/gpb_2026026","url":null,"abstract":"Keratin19 (KRT19) is the smallest type I KRT protein which is a member of the keratin family. KRT19 has been reported as a diagnostic marker in several cancers, such as lung, breast and pancreatic cancer. However, the diagnostic value and potential role of KRT19 in colorectal cancer (CRC) remain to be explored. Our study confirmed the KRT19 expression pattern in CRC through publicly database and in CRC patient tissues with immunohistochemistry (IHC) and Western blot. Then, we carried out a series of malignant phenotype assays like colony formation assay, transwell migration and invasion experiments to definite the role of KRT19 on CRC cells proliferation, migration and invasion. Co-immunoprecipitation (co-IP) and Western blot were utilized to clarify the underlying molecular mechanisms of KRT19 in CRC. The data from GEPIA and CRC specimens displayed that KRT19 expression was increased and correlated with a poor clinical outcome. Our in vitro experiments confirmed that KRT19 promoted the proliferation, migration and invasion of CRC cells. Moreover, co-IP verified the interaction between KRT19 and SIRT4. KRT19 also activated the mTOR pathway which was illustrated by Western blot. Our research provides that KRT19 may be served as a diagnostic marker for CRC, and KRT19 promotes CRC progression through mTOR pathway by interacting with SIRT4.","PeriodicalId":12514,"journal":{"name":"General physiology and biophysics","volume":"45 5","pages":"507-517"},"PeriodicalIF":1.5,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148919331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cornuside attenuates inflammatory response injury in mice with ovalbumin-induced allergic rhinitis. 山茱萸可减轻卵清蛋白诱导的变应性鼻炎小鼠的炎症反应损伤。

IF 1.5 4区生物学

General physiology and biophysics Pub Date : 2026-09-01 DOI: 10.4149/gpb_2026030

Hui Wang, Linghan Huang

{"title":"Cornuside attenuates inflammatory response injury in mice with ovalbumin-induced allergic rhinitis.","authors":"Hui Wang, Linghan Huang","doi":"10.4149/gpb_2026030","DOIUrl":"https://doi.org/10.4149/gpb_2026030","url":null,"abstract":"Allergic rhinitis (AR) is characterized by inflammation of the nasal mucosa caused by an allergic reaction. Cornuside has been reported to possess anti-allergic properties. This study aims to investigate whether cornuside can suppress AR. Mice were induced by ovalbumin (OVA) to establish AR models. The AR symptoms including sneezing and nasal rubbing were recorded, and the eosinophils in mouse mucosal tissues were analyzed by hematoxylin and eosin staining. Total leucocytes in nasal lavage fluid (NLF) were quantified under a hemocytometer. Wright's Giemsa staining was utilized to quantify eosinophils, lymphocytes, and neutrophils in NLF. Enzyme-linked immunosorbent assay was conducted to detect histamine, serum cytokines (OVA-specific IgE, OVAspecific IgG1, interleukin-4, and leukotriene C4), and serum inflammation cytokines (tumor necrosis factor-α, interleukin-6, interleukin-13, and interleukin-17). Cornuside administration significantly reduced sneezing and nasal rubbing behaviors in mice with AR. Mice with AR exhibited more inflammatory cells and higher levels of histamine, serum cytokines, and inflammatory cytokines in the blood or NLF than the control group. However, these upregulations were all reversed by the administration of cornuside. In addition, we found that cornuside treatment improved the inflammatory condition of mice with AR dose-dependently Cornuside can attenuate the inflammatory response in mice with AR.","PeriodicalId":12514,"journal":{"name":"General physiology and biophysics","volume":"45 5","pages":"539-547"},"PeriodicalIF":1.5,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148919268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Icariin alleviates abdominal aortic aneurysm by targeting CYP1B1 to inhibit M1 polarization and VSMC apoptosis. 淫羊藿苷通过靶向CYP1B1抑制M1极化和VSMC凋亡来缓解腹主动脉瘤。

IF 1.5 4区生物学

General physiology and biophysics Pub Date : 2026-09-01 DOI: 10.4149/gpb_2026025

Xiaosen Liu, Shuzhen Li, Jian Wang, Zexiang Tian

{"title":"Icariin alleviates abdominal aortic aneurysm by targeting CYP1B1 to inhibit M1 polarization and VSMC apoptosis.","authors":"Xiaosen Liu, Shuzhen Li, Jian Wang, Zexiang Tian","doi":"10.4149/gpb_2026025","DOIUrl":"https://doi.org/10.4149/gpb_2026025","url":null,"abstract":"Abdominal aortic aneurysm (AAA) is a life-threatening vascular disease with limited medical therapies. The role and targets of icariin, a flavonoid from Epimedium brevicornu Maxim, in AAA remain unclear. First, transcriptomic data from the GEO dataset GSE183464 were analyzed to screen differentially expressed genes (DEGs). DEGs were then integrated with M1 polarizationrelated genes (GeneCards) and icariin-targeted genes (CTD/SwissTargetPrediction) using Venn analysis, and the obtained candidate genes were subjected to GO and KEGG enrichment analyses. Machine learning (LASSO and Random Forest) was employed to prioritize core targets from a gene expression matrix, followed by molecular docking (AutoDock Vina) for assessing the binding affinity between icariin and Cytochrome P450 Family 1 Subfamily B Member 1 (CYP1B1), and visualizing (PyMOL and Discovery Studio) the binding modes. The effects of icariin were evaluated in THP-1-derived macrophages. Bioinformatics identified 15 candidate genes; machine learning converged on CYP1B1/BCL2L11. Molecular docking confirmed icariin's high affinity for CYP1B1. Icariin dose-dependently inhibited LPS/IFN-γ-induced M1 polarization and NF-κB activation, while CYP1B1 overexpression reversed these effects. Icariin also reduced M1-induced VSMC apoptosis. By demonstrating that icariin alleviates AAA by targeting CYP1B1 to suppress NF-κB-driven M1 polarization and VSMC apoptosis, this study establishes the icariin-CYP1B1 axis as a promising therapeutic strategy.","PeriodicalId":12514,"journal":{"name":"General physiology and biophysics","volume":"45 5","pages":"495-506"},"PeriodicalIF":1.5,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148919313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0