Frontiers in Physiology最新文献

{"title":"Therapeutic prospects and potential mechanisms of Prdx6: as a novel target in musculoskeletal disorders.","authors":"Hong Sun, Chao Xu, Zhilin Xiong, Miao Liu, Xu Ning, Yong Zhuang","doi":"10.3389/fphys.2025.1524100","DOIUrl":"https://doi.org/10.3389/fphys.2025.1524100","url":null,"abstract":"<p><p>With the global population aging, musculoskeletal disorders (MSDs) have posed significant physical and psychological health challenges for patients as well as a substantial economic burden on society. The advancements in conservative and surgical interventions for MSDs have been remarkable in recent years; however, the current treatment modalities still fall short of meeting the optimal requirements of patients. Recently, peroxiredoxin 6 (Prdx6) has gained considerable attention from researchers due to its remarkable antioxidative, anti-inflammatory, and anti-apoptotic properties. It has been found that Prdx6 is involved in multiple system diseases, including MSDs; however, the exact role of Prdx6 in MSDs is still lacking. This study aimed to summarize the structure, regulatory mechanism, and potential function of Prdx6. These findings may demonstrate Prdx6 as a novel target for inhibiting the advancement of MSDs.</p>","PeriodicalId":12477,"journal":{"name":"Frontiers in Physiology","volume":"16 ","pages":"1524100"},"PeriodicalIF":3.2,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12043587/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143985546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of resistance exercise-induced local metabolic stress in mediating systemic health and functional adaptations: could condensed training volume unlock greater benefits beyond time efficiency?","authors":"Ivan Curovic","doi":"10.3389/fphys.2025.1549609","DOIUrl":"https://doi.org/10.3389/fphys.2025.1549609","url":null,"abstract":"<p><p>The majority of \"specialised\" exercise configurations (e.g., supersets, drop sets, blood flow restriction) are being assessed as \"shortcuts\" to hypertrophy and strength improvements. However, these advanced training techniques may also offer significant benefits for systemic health and functional outcomes across recreational and clinical populations via locally induced metabolic responses. Stress-regulating mechanisms are known to enhance the body's resilience by facilitating allostasis, the process of coordinating adaptive processes in reaction to stressors such as physical training. Yet, the role of the local metabolic stress provoked by resistance exercise has not gained much research attention despite its wide potential. Positive effects are not only linked to improved muscular endurance, hypertrophy and strength via primary and secondary mechanisms, but also to the release of myokines, hormones, microRNAs, immune factors, inflammatory substances and other endocrine molecules that initiate numerous health-promoting modifications on a systemic level. Resistance exercise strategies that maximise the local accumulation of metabolites are not well defined, although high volume, close proximity to failure and shorter rests seem to be a necessity. Additionally, blood flow restriction training provides a potent alternative for inducing local acidosis, thereby triggering several pathways associated with improved immunity and physical function even in remote muscle tissues. Future research is warranted to further explore advanced resistance training techniques, as these approaches may offer comparable benefits for physical and mental health to those seen with other forms of exercise such as high-intensity interval training and heavy resistance training.</p>","PeriodicalId":12477,"journal":{"name":"Frontiers in Physiology","volume":"16 ","pages":"1549609"},"PeriodicalIF":3.2,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12045103/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143968032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of high-intensity interval training and moderate-intensity continuous training on mitochondrial dynamics in human skeletal muscle.","authors":"Yuqing Li, Wanjun Zhao, Qi Yang","doi":"10.3389/fphys.2025.1554222","DOIUrl":"https://doi.org/10.3389/fphys.2025.1554222","url":null,"abstract":"<p><p>Exercise and physical activity confer health advantages, in part, by enhancing skeletal muscle mitochondrial respiratory function. The objective of this study is to analyze the impacts of high-intensity interval training (HIIT) and moderate-intensity continuous training (MICT) on the dynamics and functionality of the mitochondrial network within skeletal muscle. 20 young male participants were assigned to either HIIT or MICT group. Initial assessments of exercise-related indicators were conducted, followed by skeletal muscle biopsies from the vastus lateralis before, 1 day after, and 6 weeks post-experiment. We utilized multi-dimensional myofiber imaging to analyze mitochondrial morphology and arrangement, and assessed citrate synthase activity, complex I activity, and dynamics-related mRNA. Both training modalities increased VO_2max, W_max, citrate synthase and complex I activities, mitochondrial content, and volume density, though the changes differed between the two groups. 6 weeks training induced remodeling of the mitochondrial network within skeletal muscle. Before training, the network appeared sparse and punctate. After MICT, it adopted a grid-like structure with partially robust longitudinal connections. In contrast, HIIT resulted in a less obvious grid structure but showed a stronger longitudinally oriented network. Training also increased mRNA expression of mitochondrial fusion proteins and decreased fission protein expression, with these effects being more pronounced in HIIT. Similarly, peroxisome proliferator-activated receptor γ coactivator 1-alpha mRNA expression showed a comparable trend, though the changes differed between 1 day and 6 weeks of training. In conclusion, HIIT and MICT induce distinct mitochondrial adaptation in skeletal muscle, reflected in different network remodeling and molecular pathways. These findings may be due to HIIT's more pronounced effect on mitochondrial dynamics or respiratory function, but the study has only conducted preliminary observational experiments and further evidence is required for confirmation.</p>","PeriodicalId":12477,"journal":{"name":"Frontiers in Physiology","volume":"16 ","pages":"1554222"},"PeriodicalIF":3.2,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12043657/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143987703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of time of day on visual reaction time performance in boxers: evaluation in terms of chronotype.","authors":"Kemal Kurak, İsmail İlbak, Stefan Stojanović, Ramazan Bayer, Yunus Emre İlbak, Krzysztof Kasicki, Tadeusz Ambroży, Łukasz Rydzik, Wojciech Czarny","doi":"10.3389/fphys.2025.1589740","DOIUrl":"https://doi.org/10.3389/fphys.2025.1589740","url":null,"abstract":"<p><strong>Introduction: </strong>Considering the impact of individual differences on athletes' performance, chronotype emerges as a crucial variable in training program design. Chronotype influences an individual's ability to achieve peak physical and cognitive performance at different times of the day based on their biological rhythms. While numerous studies have explored the relationship between chronotype and physical performance, its effect on reaction time performance remains insufficiently investigated. In sports, where reaction time is a key determinant-such as in boxing-understanding this relationship could contribute to the personalization of training programs. Therefore, the aim of this cross-sectional study was to examine how the visual reaction performance of active boxers varies at different times of the day based on their chronotypes.</p><p><strong>Methods: </strong>Twenty-four active boxers participated in the study. Their chronotypes were determined using the Morningness-Eveningness Questionnaire, categorizing them as either morning type (M-type) or evening type (E-type). The participants were divided into two groups: M-type (n = 12) and E-type (n = 12). Each participant completed a visual reaction time (VRT) performance test at three different times of the day: morning (09:00 h), afternoon (13:00 h), and evening (17:00 h).</p><p><strong>Results: </strong>The findings revealed a statistically significant group × time interaction effect on VRT performance (p < 0.01). M-type athletes showed a significant decline in VRT performance during the evening compared to the morning and afternoon. In contrast, E-type athletes demonstrated significantly better performance in the evening compared to the morning.</p><p><strong>Conclusions: </strong>Boxers' visual reaction time performance varies throughout the day depending on their chronotype. These results suggest that coaches and exercise specialists should consider athletes' chronotypes when designing training programs focused on reaction time enhancement. To optimize performance, it is recommended that M-type athletes conduct such training sessions in the morning, while E-type athletes should train in the evening, when their reaction time performance tends to peak.</p>","PeriodicalId":12477,"journal":{"name":"Frontiers in Physiology","volume":"16 ","pages":"1589740"},"PeriodicalIF":3.2,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12040622/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143978572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Conceptualizing myocardial contractility as an emergent property that characterizes myocardial contraction.","authors":"Serena Y Kuang, Gorune Geloian","doi":"10.3389/fphys.2025.1499536","DOIUrl":"https://doi.org/10.3389/fphys.2025.1499536","url":null,"abstract":"<p><p>Myocardial contractility (MC) is a fundamental concept that is widely used to describe the cardiac muscles' mechanical function, yet its definitions in textbooks and literature are vague, inconsistent, and often contradictory. In this article, we categorize these many issues into five groups and conducts a conceptual analysis to redefine MC from a broader, more comprehensive perspective. We propose a functional, three-domain framework of MC consisting of capacity/resource, adaptability, and ability (force (F) and/or velocity (V) generated during muscle contraction), emphasizing the dynamic, non-linear interactions among the three domains and their clinical significance. Specifically, we highlight how interventions targeting MC may produce non-linear effects, suggesting a shift toward optimizing resource use rather than maximizing outputs (i.e., F and/or V of myocardial contraction, the outputs of the ability domain), which could potentially reduce the complications of positive inotropic interventions. We also discuss the implications of several new conceptual developments as the byproducts of the three-domain MC framework. Additionally, we identify system-level emergent properties of MC briefly, including contraction efficiency, circadian rhythm-dependence, temperature-dependence, and history-dependence, with implications for cardiac muscle research, exercise training, and clinical decision-making. The three-domain functional framework of MC resolves the inconsistencies in definitions, differentiates MC from cardiac performance, and offers a structured perspective for facilitating both experimental studies and therapeutic strategies.</p>","PeriodicalId":12477,"journal":{"name":"Frontiers in Physiology","volume":"16 ","pages":"1499536"},"PeriodicalIF":3.2,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12040998/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143998016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Utilizing bioinformatics to identify biomarkers and analyze their expression in relation to immune cell ratios in femoral head necrosis.","authors":"Dongchen Li, Zhilong Huang, Teng Ma, Yu Su, Zhao Li, Liang Sun, Ming Li, Zhong Li, Yao Li, Qian Wang, Yao Lu","doi":"10.3389/fphys.2025.1373721","DOIUrl":"https://doi.org/10.3389/fphys.2025.1373721","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Background: &lt;/strong&gt;Necrosis of the Femoral Head (NFH) represents a challenging orthopedic condition, characterized by elusive early detection and rapid progression, predominantly in the middle-aged demographic. Current research on the pathophysiological and immunoregulatory mechanisms underpinning immune cell infiltration in NFH is sparse. This study employs bioinformatics analysis of publicly available RNA sequencing databases to elucidate the pivotal molecules and pathways implicated in NFH progression.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;The NFH-related dataset GSE123568 was obtained from the Gene Expression Omnibus (GEO). Subsequently, CIBERSORT was utilized to assess the proportion and distribution of immune cell types, followed by the identification of critical Hub immune cells using LASSO and RFE algorithms. The dataset GSE123568 was then explored to identify significantly differentially expressed genes (DEGs). These genes were further refined by intersecting with death-associated genes reported in existing literature. GO and KEGG pathway enrichment analyses were conducted to elucidate their underlying molecular mechanism. A protein-protein interaction (PPI) network was constructed using the STRING database and visualized via Cytoscape. Hub genes were identified using the CytoHubba plugin, followed by enrichment analysis, and their expression levels were evaluated using the ROC curve. In addition, we performed expression data visualization and ROC curve analysis on the external dataset GSE74089 to further evaluate the discriminative power of the hub genes. Moreover, the study analyzed the correlation between the identified hub genes and Hub immune cells. Finally, we verified the hub genes utilizing real-time fluorescence quantitative polymerase chain reaction (RT-qPCR) and immunohistochemistry.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;Four types of immune cells (Neutrophil, Mast cell resting, Myeloid dendritic cell activated, Macrophage M0) were identified. Fourteen pivotal genes (BCL2L1, BIRC2, NFKBIA, XIAP, CFLAR, AKT1, BIRC3, IKBKB, RIPK1, CASP8, TNFRSF1A, IL1B, CASP1, STAT3) were identified, and the findings were validated using the external dataset GSE74089. Among these, STAT3 exhibited the most pronounced positive correlation with neutrophils (r = 0.6804, p = 3.525e-05). Conversely, XIAP displayed the most significant negative correlation with Myeloid dendritic cell activated (r = -0.3610, p = 0.04003). In experiments, the experimental outcomes for five hub genes (CASP8, TNFRSF1A, AKT1, XIAP and STAT3) were congruent with the results obtained from bioinformatics analysis.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Conclusion: &lt;/strong&gt;Our study identified CASP8, TNFRSF1A, AKT1, XIAP, STAT3 and BCL2L1 as potential biomarkers for NFH patients and elucidated the immune cell types with the strongest association to these markers. These insights may be crucial for the early diagnosis, understanding of the pathophysiological mechanisms, and the development of treatment","PeriodicalId":12477,"journal":{"name":"Frontiers in Physiology","volume":"16 ","pages":"1373721"},"PeriodicalIF":3.2,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12040900/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144000553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Customizing individual heat mitigation strategies to optimize performance in elite athletes.","authors":"Yasuki Sekiguchi, William M Adams, Yuri Hosokawa, Courteney L Benjamin, Rebecca L Stearns, Robert A Huggins, Douglas J Casa","doi":"10.3389/fphys.2025.1380645","DOIUrl":"https://doi.org/10.3389/fphys.2025.1380645","url":null,"abstract":"<p><p>The aim of this review is twofold: 1) provide a brief discussion surrounding the interindividual variability that has been observed within the context of heat acclimation/acclimatization, body cooling, and hydration strategies, and 2) provide the reader with a practitioner-focused approach for creating individualized heat mitigation strategies. Considering individual variability for heat acclimation and heat acclimatization, various body cooling strategies, and hydration assessment/fluid replacement is important to maximize effects of these strategies, which lead to better performance and health outcomes. There are many factors to consider, and comprehensive approaches are required. The evidenced-informed decision is critical when making an individual approach, and data will help to make decisions effectively. It is important to keep adjusting the approach based on observed data as data is useful information to check if the approach is effective. Specific considerations to individualize the plan are discussed in this review.</p>","PeriodicalId":12477,"journal":{"name":"Frontiers in Physiology","volume":"16 ","pages":"1380645"},"PeriodicalIF":3.2,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12040879/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143998017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MRI assessment of sacral injury location and analysis of influencing factors after high-intensity focused ultrasound ablation for patients with uterine fibroids.","authors":"Ao Zhou, Xin Feng, Furong Lv, Yunyue Tan, Yuhang Liu, Zhibo Xiao","doi":"10.3389/fphys.2025.1523018","DOIUrl":"https://doi.org/10.3389/fphys.2025.1523018","url":null,"abstract":"<p><strong>Purpose: </strong>Exploration of the location of sacral injuries following ultrasound-guided high-intensity focused ultrasound (USgHIFU) ablation for uterine fibroids and analysis of its influencing factors.</p><p><strong>Methods: </strong>A retrospective analysis was conducted on 663 patients with uterine fibroids treated by USgHIFU ablation. Patients with vertebral injuries were identified based on postoperative MRI images, with specific locations of the injuries documented. Additionally, the condition of muscle damage around the vertebral body was assessed. Patients were divided into Upper group and Lower group based on the location of vertebral injuries. Univariate and multivariate logistic regression analyses were conducted to identify the influencing factors. The χ² test was used to explore the relationship between the location of vertebral injuries and postoperative clinical adverse events, as well as muscle damage.</p><p><strong>Results: </strong>Postoperative MRI examinations revealed that 42.3% (281/663) of the patients experienced vertebral injuries, which were localized to the range from L5 to S5. The injuries from L5 to S2 were classified as Upper group, accounting for 45.2% (127/281), while those from S3 to S5 were classified as Lower group, accounting for 54.8% (154/281). Multivariate analysis revealed that the distance from the ventral side of the fibroid to the abdominal wall skin, uterine position, and T2WI signal intensity were positively correlated with the location of sacral injuries (p < 0.05). Additionally, the location of sacral injuries was significantly associated with the occurrence of postoperative sacrococcygeal pain (p < 0.05). 162 patients (57.6%) with sacral injury were accompanied by piriformis and gluteus maximus muscle injuries, with piriformis injury accounting for 95.06%. The location of sacral injury was significantly correlated with piriformis injury (p < 0.05).</p><p><strong>Conclusion: </strong>Postoperative MRI images of some patients with uterine fibroids treated with USgHIFU ablation show vertebral and surrounding muscle injuries, mainly involving sacrum and piriformis. For those with a retroverted uterus, a large distance between the ventral side of the fibroid and the abdominal wall, or fibroids exhibiting high signals on T2-weighted images (T2WI), the location of postoperative sacral injuries tends to be more inferior. Additionally, these patients face an increased risk of concurrent piriformis injury and a higher likelihood of experiencing sacrococcygeal pain.</p>","PeriodicalId":12477,"journal":{"name":"Frontiers in Physiology","volume":"16 ","pages":"1523018"},"PeriodicalIF":3.2,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12041009/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143979112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nailfold capillary patterns in ballgame and endurance athletes.","authors":"Takeshi Otsuki, Kazuya Suwabe, Toru Yoshikawa, Kiwamu Kotani, Asako Zempo-Miyaki","doi":"10.3389/fphys.2025.1568972","DOIUrl":"https://doi.org/10.3389/fphys.2025.1568972","url":null,"abstract":"<p><strong>Purpose: </strong>Nailfold capillary patterns can be observed noninvasively using light microscopy. Nailfold capillaries are straight, U-shaped, and densely looped in healthy individuals and may be altered by disease or lifestyle factors, such as diet. However, the effects of daily physical activity and exercise training on nailfold capillary patterns remain unclear. This study aimed to examine the effects of exercise training on nailfold capillary patterns by investigating these patterns in endurance athletes, ballgame athletes, and sedentary healthy men.</p><p><strong>Methods: </strong>Five healthy men participated in nailfold capillary pattern measurements on three consecutive days to test the reproducibility and bilateral differences in the nailfold capillary loop density, length, and width measured using light microscopy and a commercial analysis system. The nailfold capillaries of 10 endurance athletes (endurance group; eight long-distance runners and two triathletes), 10 ballgame athletes (ballgame group; seven soccer players and three basketball players), and nine sedentary healthy men (sedentary group) were then examined using light microscopy.</p><p><strong>Results: </strong>The day-to-day coefficient of variation for the nailfold capillary loop density, length, and width were 4.9% ± 1.6%, 7.5% ± 1.3%, and 4.2% ± 1.5%, respectively. No significant differences in these measurements were observed between the dominant and non-dominant hands. Nailfold capillary density was greater in the ballgame group than in the endurance and sedentary groups. Capillary loop length was shorter in the ballgame group than in the endurance and sedentary groups. No significant differences in capillary loop density and length were observed between the endurance and sedentary groups. No significant intergroup differences were observed in capillary loop width.</p><p><strong>Conclusion: </strong>These results suggest that the nailfold capillary patterns of male ballgame athletes differ from those of endurance athletes and sedentary healthy men. Ballgame training may affect nailfold capillary patterns in sedentary healthy men.</p>","PeriodicalId":12477,"journal":{"name":"Frontiers in Physiology","volume":"16 ","pages":"1568972"},"PeriodicalIF":3.2,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12040917/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143973174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Testosterone levels positively linked to muscle mass but not strength in adult males aged 20-59 years: a cross-sectional study.","authors":"Wei Zhang, Zhaohui Cui, Dayong Shen, Li Gao, Qingyun Li","doi":"10.3389/fphys.2025.1512268","DOIUrl":"https://doi.org/10.3389/fphys.2025.1512268","url":null,"abstract":"<p><strong>Background: </strong>The relationship between testosterone levels and muscle mass and strength remains contentious. This study aimed to explore the relationship among total serum testosterone levels, muscle mass, and strength in young to middle-aged adults.</p><p><strong>Methods: </strong>The analysis included 4,495 participants (age 39.2 ± 0.2 years, mean ± SE) from the National Health and Nutrition Examination Survey conducted between 2011 and 2014. Weighted regression models were used to assess the association of testosterone levels with muscle mass and strength.</p><p><strong>Results: </strong>For male participants, log₂-transformed testosterone levels were positively associated with appendicular lean mass adjusted for body mass index (β: 0.05, 95% confidence interval [CI]: 0.03-0.07, <i>P</i> < 0.001) and negatively associated with low muscle mass (odds ratio: 0.40, 95% CI: 0.24-0.67, <i>P</i> = 0.006). However, no significant association was found between testosterone levels and grip strength (β: 1.16, 95% CI: 0.26 to 2.58, <i>P</i> = 0.086) or low muscle strength (odds ratio: 0.51, 95% CI: 0.25-1.04, <i>P</i> = 0.059). For female participants, no significant association was observed between testosterone levels and muscle mass (β: 0.01, 95% CI: 0.02 to -0.01, <i>P</i> = 0.294) or muscle strength (β: 0.14, 95% CI: 0.45 to 0.73, <i>P</i> = 0.508). Restricted cubic spline analysis revealed a linear relationship between total testosterone levels and appendicular lean mass adjusted for body mass index in male participants (nonlinear: <i>P</i> = 0.367).</p><p><strong>Conclusion: </strong>Our study indicates that testosterone levels are positively associated with muscle mass but not with muscle strength in young to middle-aged males.</p>","PeriodicalId":12477,"journal":{"name":"Frontiers in Physiology","volume":"16 ","pages":"1512268"},"PeriodicalIF":3.2,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12037588/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143967507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}