Frontiers in Molecular Biosciences最新文献

{"title":"Performance and clinical implications of non-invasive prenatal testing for rare chromosomal abnormalities: a retrospective study of 94,125 cases.","authors":"Haimei Qi, Haijun Chen, Zhuling Zhang, Jinhui Gan, Huifeng Liu, Xianping Yuan, Fuyu Luo, Junkun Chen, Shichun Shen","doi":"10.3389/fmolb.2025.1645223","DOIUrl":"10.3389/fmolb.2025.1645223","url":null,"abstract":"<p><strong>Background: </strong>Non-invasive prenatal testing (NIPT) has demonstrated robust performance in detecting common trisomies and copy number variations. However, its clinical utility for rare chromosomal abnormalities (RCAs) remains controversial due to low positive predictive value (PPV).</p><p><strong>Methods: </strong>This study retrospectively analyzed the data of 94,125 cases that underwent NIPT at Ganzhou Maternal and Child Health Hospital in China. This dataset was used to evaluate NIPT performance in RCAs detection and track pregnancy outcomes of positive cases.</p><p><strong>Results: </strong>In the cohort of 94,125 pregnancies undergoing NIPT, 336 cases (0.36%) were found to carry RCAs. Among them, 102 cases underwent validation through karyotyping and/or chromosome microarray analysis. Of the 102 validated cases, seven were true positives (PPV = 6.86%). Additionally, 3 cases exhibited uniparental disomy consistent with the NIPT-reported chromosomal anomalies. Of 268 singleton neonates, 68 (25.37%) were small-for-gestational-age.</p><p><strong>Conclusion: </strong>This study found that most NIPT-detected RCAs were associated with maternal age, while Trisomy seven occurred independently of maternal age. Concurrent use of karyotyping and chromosome microarray analysis, rather than karyotyping alone, mitigates culture-related bias and enhances the PPV. Both biological and methodological factors contribute to the low PPV of NIPT for RCAs. Despite a low PPV, pregnancies with NIPT-indicated RCAs showed elevated risks of fetal loss, small-for-gestational-age, and uniparental disomy, though not preterm birth. Thus, NIPT-detected RCAs retain clinical significance for risk stratification and pregnancy management.</p>","PeriodicalId":12465,"journal":{"name":"Frontiers in Molecular Biosciences","volume":"12 ","pages":"1645223"},"PeriodicalIF":3.9,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12404953/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145000132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pioneering contribution of Professor Bruce Ames to early development in biochemical aspects of oxidatively generated damage to DNA.","authors":"Jean Cadet, J Richard Wagner","doi":"10.3389/fmolb.2025.1636255","DOIUrl":"10.3389/fmolb.2025.1636255","url":null,"abstract":"<p><p>The first part of the memorial review article is devoted to a retrospective of selected topics that were the subject of pioneering studies over the period 1985-2025 by Professor Bruce Ames. Major efforts were made to develop accurate and sensitive assays including HPLC coupled with electrochemical detection for monitoring the formation of 8-oxo-7,8-dihydroguanine in isolated cells and animal tissues. Special attention was provided to the minimization of artefactual oxidation of DNA that occurs during sample preparation. Complementary information on the biological relevance of 8-oxo-7,8-dihydroguanine and 5,6-dihydroxy-5,6-dihydrothymine was gained from the non-invasive measurement of the oxidized bases and nucleosides in various mammalian fluids. The second part of this review focuses on the current situation concerning the formation of oxidized bases in cellular DNA produced under various conditions of oxidative stress and enzymatic ten-eleven TET-oxidation of 5-methylcytosine. The analysis of DNA base modifications by LC-MS/MS is the gold standard for the quantitative monitoring of base oxidation products in both DNA and several body fluids; oxidizing conditions that may not be suitable for biological studies. Low levels of oxidatively-induced lesions in cells are difficult to assess by chromatographic and MS methods because of a significant increase in the yields of oxidized bases/nucleosides above the background level including a significant contribution of adventitious oxidation reactions that cannot be totally suppressed. In a complementary way, the application of modified versions of the comet assay and alkaline elution techniques that target general classes of DNA lesions provides a more global account of damage although it gives less structural information about DNA damage formed under chronic exposure to mild oxidizing conditions.</p>","PeriodicalId":12465,"journal":{"name":"Frontiers in Molecular Biosciences","volume":"12 ","pages":"1636255"},"PeriodicalIF":3.9,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12405702/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145000110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular mechanisms and therapeutic advances of peritubular capillary neogenesis in acute kidney injury.","authors":"Yuming Ding, Linmei Gao, Yi Chen, Yanheng Qiao, Bo Yang","doi":"10.3389/fmolb.2025.1643838","DOIUrl":"10.3389/fmolb.2025.1643838","url":null,"abstract":"<p><p>Acute kidney injury is a clinical syndrome characterized by a rapid decline in renal function, driven by pathological mechanisms such as renal tubular epithelial cell injury, inflammatory responses, and microcirculatory dysfunction. In recent years, the role of angiogenesis in AKI recovery and regeneration has gained increasing attention. Angiogenesis plays a dual role in tissue repair and pathological remodeling, exhibiting complex spatiotemporal dynamics during AKI progression. This review synthesizes recent advances in understanding the role of angiogenesis in AKI, with the aim of identifying potential diagnostic and therapeutic strategies. Studies indicate that the ischemic-hypoxic microenvironment following AKI activates key signaling pathways, including hypoxia-inducible factor-1α, which subsequently upregulates vascular endothelial growth factor and angiopoietins, thereby modulating intrarenal angiogenesis. Controlled angiogenesis may enhance regional perfusion, mitigate hypoxic injury, and facilitate tubular repair, whereas excessive or dysregulated angiogenesis can contribute to maladaptive vascular remodeling and fibrotic progression. Current research efforts focus on therapeutic strategies aimed at modulating angiogenesis, such as exogenous VEGF administration, endothelial progenitor cell transplantation, and Notch signaling modulation, to promote functional vascular regeneration. However, the precise role of angiogenesis varies across different AKI phases (acute vs recovery), and its interactions with inflammatory and fibrotic pathways remain incompletely understood. Further elucidation of these mechanisms is essential for developing targeted therapeutic interventions.</p>","PeriodicalId":12465,"journal":{"name":"Frontiers in Molecular Biosciences","volume":"12 ","pages":"1643838"},"PeriodicalIF":3.9,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12404958/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145000121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Shared transcriptional regulators and network rewiring identify therapeutic targets linking type 2 diabetes mellitus and hypertension.","authors":"Claudia Desireé Norzagaray-Valenzuela, Marco Antonio Valdez-Flores, Josue Camberos-Barraza, Alberto Kousuke De la Herrán-Arita, Juan Fidel Osuna-Ramos, Javier Magaña-Gómez, Carla Angulo-Rojo, Alma Marlene Guadrón-Llanos, Katia Aviña-Padilla, Loranda Calderón-Zamora","doi":"10.3389/fmolb.2025.1621413","DOIUrl":"10.3389/fmolb.2025.1621413","url":null,"abstract":"<p><strong>Background: </strong>Type 2 diabetes mellitus (T2DM) and Hypertension (HTN) frequently coexist and synergistically exacerbate vascular and immune dysfunction. Despite their clinical interrelation, these diseases have traditionally been studied in isolation, and the molecular mechanisms underlying their comorbidity remain poorly understood. This study aimed to uncover shared transcriptional programs and disease-specific regulatory networks contributing to cardiometabolic dysfunction.</p><p><strong>Methods: </strong>We systematically selected transcriptomic datasets and employed an integrative systems biology approach that combined differential gene expression analysis, co-expression network construction, protein-protein interaction mapping, transcription factor activity inference, and network rewiring analysis. Functional enrichment analyses were conducted to elucidate biological processes associated with disease-specific modules.</p><p><strong>Results: </strong>We identified distinct regulatory modules: ME3 in T2DM, enriched in metabolic stress response, intracellular trafficking, and inflammation, and ME7 in HTN, enriched in immune response and vascular remodeling. Protein interaction networks revealed key hub genes such as <i>GNB1</i>, <i>JAK1</i>, and <i>RPS3</i> as T2DM-specific hubs, while MAPK1, <i>BUB1B</i>, and RPS6 were central in HTN. Network rewiring analysis showed condition-specific changes in gene connectivity, particularly in <i>ST18</i> and <i>SLBP</i> gaining prominence in T2DM, and <i>SLC16A7</i> and <i>SPX</i> showing decreased connectivity in HTN. Notably, transcription factor activity analysis revealed shared regulators <i>HNF4A</i> and <i>STAT2</i> implicated in inflammation, oxidative stress, and vascular remodeling, highlighting a transcriptional convergence between the two conditions.</p><p><strong>Conclusion: </strong>This study provides novel insights into the molecular crosstalk between T2DM and HTN by identifying conserved transcriptional regulators and rewired gene networks. Our findings support the existence of a shared regulatory architecture underlying cardiometabolic comorbidity and suggest promising diagnostic and therapeutic targets for precision medicine.</p>","PeriodicalId":12465,"journal":{"name":"Frontiers in Molecular Biosciences","volume":"12 ","pages":"1621413"},"PeriodicalIF":3.9,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12404935/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145000185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The endothelial layer formation in the presence of AuNPs/CdSe/TaNPs-loaded PLCL/PVP-based electrospun nanofibers.","authors":"Magdalena Lasak, Viraj P Nirwan, Dorota Kuc-Ciepluch, Ryszard Tomasiuk, Igor Chourpa, Amir Fahmi, Karol Ciepluch","doi":"10.3389/fmolb.2025.1638442","DOIUrl":"10.3389/fmolb.2025.1638442","url":null,"abstract":"<p><strong>Background: </strong>Electrospun nanofibers, which are becoming increasingly popular in biomedicine, can directly or indirectly affect the properties and formation of the edothelial layer. This effect can be both toxic and pro-stimulatory. Therefore, in this study, electrospun nanofibers PLCL/PVP composed of biodegradable and biocompatible L-lactide-block-<i>ϵ</i>-caprolactone copolymer (PLCL, 70:30) blended with polyvinylpyrrolidone (PVP), containing <i>in situ</i> synthesized PVP different types of nanoparticles - gold (AuNPs), cadmium selenide (CdSe QDs) or tantalum (TaNPs), were investigated. Understanding how different modifications of nanofibers can affect the formation of the endothelial layer is crucial to using them as tools in tissue regeneration.</p><p><strong>Methods: </strong>electrospun nanofibers with gold (AuNPs), cadmium selenide (CdSe QDs) or tantalum (TaNPs), were synthesized and physico-chemical characteristic were caried out. Cytotoxicity and prostimulatory effect of nanofibers on Primary Human Umbilical Vein Endothelial Cells were tested by microscopic and spectrofluorescence techniques.</p><p><strong>Results: </strong>The endothelial layer forms to 75% confluence (after 24 h) and reaches 100% after 72 h when no nanofibers are present. A slower formation of the endothelial layer is seen in the presence of PLCL/PVP nanofibers (60%) and (80%) after 72 h. The introduction of various nanoparticles into the nanofibers caused changes in the morphology and rate of endothelial layer formation. In the presence of nanofibers modified with AuNPs after 72 h it reached only 40%. A similar effect was obtained for PLCL/PVP-CdSe QDs. In the case of PLCL/PVP-TaNPs, after 48 h 90% and after 72 h 100%. The tested nanofibers did not show toxic behavior towards the formed HUVEC cell monolayer. All of the tested nanofibers, except PLCL/PVP-TaNPs, induced increased HUVEC cells layer permeability, which resulted in increased translocation of fluorescently labeled dextran from 20% to 50%.</p><p><strong>Conclusion: </strong>It was estimated that the effect of nanofibers on the formation of the endothelial layer can be direct, where cells contact the nanofibers and thus the growth of the endothelium is hindered. Additionally, the uptake of biological fluid components can have an indirect effect on endothelial cells, their adhesion and growth. Among the tested nanofibers, non-toxic PLCL/PVP-TaNPs seem to be particularly promising due to safety issues and the possibility of using them as effective scaffolds.</p>","PeriodicalId":12465,"journal":{"name":"Frontiers in Molecular Biosciences","volume":"12 ","pages":"1638442"},"PeriodicalIF":3.9,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12399384/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144991947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Anticarcinogenic cationic peptides derived from tryptic hydrolysis of β-lactoglobulin.","authors":"Eman Ibrahem, Ali Osman, Hefnawy Taha, Mohamed F Abo El-Maati, Basel Sitohy, Mahmoud Sitohy","doi":"10.3389/fmolb.2025.1660539","DOIUrl":"10.3389/fmolb.2025.1660539","url":null,"abstract":"<p><p>[This corrects the article DOI: 10.3389/fmolb.2024.1444457.].</p>","PeriodicalId":12465,"journal":{"name":"Frontiers in Molecular Biosciences","volume":"12 ","pages":"1660539"},"PeriodicalIF":3.9,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12399878/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144991890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of anoikis-related genes classification patterns and immune infiltration characterization in chronic rhinosinusitis with nasal polyps based on machine learning.","authors":"Ziqi Chen, Lingmei Qu, Qing Hao, Shuang Teng, Shuo Liu, Qin Wu, Hongtian Yi, Xianji Shen, Liang Li, Zhaonan Xu, Yanan Sun","doi":"10.3389/fmolb.2025.1624300","DOIUrl":"10.3389/fmolb.2025.1624300","url":null,"abstract":"<p><strong>Introduction: </strong>Chronic rhinosinusitis with nasal polyps (CRSwNP) is characterized by stromal edema, albumin deposition, and pseudocyst formation. Anoikis, a process in which cells detach from the correct extracellular matrix, disrupts integrin junctions, thereby inhibiting improperly proliferating cells from growing or adhering to an inappropriate matrix. Although anoikis is implicated in immune regulation and CRSwNP pathogenesis, its specific mechanistic role remains poorly defined.</p><p><strong>Methods: </strong>The GSE136825 and GSE179625 datasets were obtained from the GEO database and 338 anoikis-related genes (ARGs) were extracted from the literature and databases. Immune cell infiltration was analysed using the CIBERSORT algorithm. CRSwNP samples were classified via consensus clustering. Key ARGs were identified through machine learning. The diagnostic performance of candidate genes was evaluated using Receiver Operating Characteristic (ROC) analysis. Functional annotation was performed based on Gene Ontology (GO) terms, and pathway enrichment analysis was conducted using the Kyoto Encyclopedia of Genes and Genomes (KEGG) database. Regulatory networks were visualized using NetworkAnalyst and Cytoscape. Experimental validation included quantitative real-time reverse-transcription PCR (qRT-PCR), immunohistochemistry (IHC), and immunofluorescence (IF) in human tissues.</p><p><strong>Results: </strong>Consensus clustering stratified CRSwNP patients into two distinct anoikis-related clusters. Machine learning identified four key genes: CDH3, PTHLH, PDCD4, and androgen receptor (AR). The nomogram model demonstrated high diagnostic accuracy, with an area under the receiver operating characteristic curve (AUC) >0.90. Immune infiltration analysis revealed differential immune microenvironments between clusters, with AR overexpressed in cluster 1 and PTHLH in cluster 2. Network analysis identified 862 drugs or compounds targeting AR. Experimental validation confirmed consistency between bioinformatics predictions and tissue-level expression patterns.</p><p><strong>Conclusion: </strong>This study delineates two anoikis-related molecular subtypes of CRSwNP and identifies AR and PTHLH as cluster-specific biomarkers. These findings provide novel insights for personalized therapy, drug screening, and immunomodulatory strategies in CRSwNP.</p>","PeriodicalId":12465,"journal":{"name":"Frontiers in Molecular Biosciences","volume":"12 ","pages":"1624300"},"PeriodicalIF":3.9,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12394058/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144949042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preoperative serum inflammatory markers in the prognostic assessment of hepatocellular carcinoma resection in stages I/II.","authors":"Fan Liu, Yuan Xiang, Hao Xu, Xiaoxue Xu","doi":"10.3389/fmolb.2025.1640390","DOIUrl":"10.3389/fmolb.2025.1640390","url":null,"abstract":"<p><strong>Background: </strong>Hepatocellular carcinoma (HCC) remains a global health challenge, with early-stage resection offering the best chance for improved outcomes. However, limitations of the TNM staging system highlight the need for additional prognostic tools. This study evaluates the prognostic value of preoperative serum inflammatory markers in patients with stage I/II HCC undergoing surgical resection.</p><p><strong>Methods: </strong>A retrospective cohort study was conducted on 410 HCC patients (stage I/II) who underwent surgical resection at the Affiliated Hospital of North Sichuan Medical College between November 2011 and March 2020. Clinical and serological data, including neutrophil-to-lymphocyte ratio (NLR), platelet-to-lymphocyte ratio (PLR), and platelet-to-neutrophil ratio (PNR), were analyzed. Prognostic factors for overall survival (OS) were identified through univariate and multivariate Cox regression analyses. A nomogram was developed to predict 1-year, 3-year, and 5-year OS, with its performance assessed using ROC curves, calibration plots, and decision curve analysis (DCA). Kaplan-Meier survival curves were used to compare risk groups, and the model's predictive efficacy was evaluated against the AJCC 8th Edition TNM staging system.</p><p><strong>Results: </strong>Multivariate Cox regression identified NLR, PLR, ALBI score, AFP levels, and HBeAg status as independent prognostic factors for OS. The nomogram demonstrated superior discriminatory power (AUC: 0.78, 0.74, and 0.71 for 1-, 3-, and 5-year OS, respectively) compared to TNM staging. Kaplan-Meier analysis revealed significantly worse OS in the high-risk group (log-rank <i>p</i> < 0.001). The nomogram outperformed the AJCC TNM system in both discrimination and clinical utility, as validated by decision curve analysis and the Integrated Discrimination Improvement Index.</p><p><strong>Conclusion: </strong>Preoperative serum inflammatory markers, when integrated with traditional TNM staging, significantly improve prognostic accuracy for stage I/II HCC patients undergoing surgical resection. The developed nomogram provides a practical tool for individualized risk stratification and may guide postoperative management to improve patient outcomes.</p>","PeriodicalId":12465,"journal":{"name":"Frontiers in Molecular Biosciences","volume":"12 ","pages":"1640390"},"PeriodicalIF":3.9,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12394059/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144949076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RetinalVasNet: a deep learning approach for robust retinal microvasculature detection.","authors":"Zhaomin Yao, Cengcong Xing, Gancheng Zhu, Weiming Xie, Zhiguo Wang, Guoxu Zhang","doi":"10.3389/fmolb.2025.1562608","DOIUrl":"10.3389/fmolb.2025.1562608","url":null,"abstract":"<p><strong>Introduction: </strong>The retinal microvasculature has been definitively linked to a variety of diseases, such as ophthalmological, cardiovascular, and other medical conditions. Precisely identifying the retinal microvasculature is crucial for early detection and monitoring of these diseases. While the majority of existing neural network-based research has primarily focused on utilizing the green channel of fundus images for vessel segmentation, it is important to acknowledge the potential value of other channels in this process.</p><p><strong>Methods: </strong>This study introduces RetinalVasNet, a new method aimed at enhancing the accuracy and effectiveness of retinal vascular segmentation by implementing a sophisticated neural network architecture and incorporating multi-channel fundus images.</p><p><strong>Results: </strong>Our experimental results demonstrate that RetinalVasNet outperforms previous research in most performance metrics.</p><p><strong>Discussion: </strong>The findings suggest that each channel provides unique contributions to the vascular segmentation process, emphasizing the importance of incorporating multiple channels for accurate and comprehensive segmentation.</p>","PeriodicalId":12465,"journal":{"name":"Frontiers in Molecular Biosciences","volume":"12 ","pages":"1562608"},"PeriodicalIF":3.9,"publicationDate":"2025-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12390797/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144948991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrating thermodynamics and molecular kinetics into western blotting teaching.","authors":"Zhiyong Wang, Min Wang, Yan Yu, Yanxin Lu, Qiang Xia, Pei Wei","doi":"10.3389/fmolb.2025.1640740","DOIUrl":"10.3389/fmolb.2025.1640740","url":null,"abstract":"","PeriodicalId":12465,"journal":{"name":"Frontiers in Molecular Biosciences","volume":"12 ","pages":"1640740"},"PeriodicalIF":3.9,"publicationDate":"2025-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12390805/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144949021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}