{"title":"Integrated multi-omics analysis reveals the functional and prognostic significance of lactylation-related gene PRDX1 in breast cancer.","authors":"Qinqing Wu, Heng Cao, Jiangdong Jin, Dongxu Ma, Yixiao Niu, Yanping Yu, Xiang Wang, Yiqin Xia","doi":"10.3389/fmolb.2025.1580622","DOIUrl":"https://doi.org/10.3389/fmolb.2025.1580622","url":null,"abstract":"<p><strong>Background: </strong>Breast cancer (BRCA) is a significant threat to women's health worldwide, and its progression is closely associated with the tumor microenvironment and gene regulation. Lactylation modification, as a key epigenetic mechanism in cancer biology, has not yet been fully elucidated in the context of BRCA. This study examines the regulatory mechanisms of lactylation-related genes (LRGs), specifically PRDX1, and their prognostic significance in BRCA.</p><p><strong>Methods: </strong>We integrated data from multiple databases, including Genome-Wide Association Study (GWAS) summary statistics, single-cell RNA sequencing, spatial transcriptomics, and bulk RNA sequencing data from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases. Using Summary-based Mendelian Randomization (SMR) analysis, we identified LRGs associated with BRCA and comprehensively analysed the expression patterns of PRDX1, cell-cell communication networks, and spatial heterogeneity. Furthermore, we constructed and validated a prognostic model based on the gene expression profile of PRDX1-positive monocytes, evaluating it through Cox regression and LASSO regression analyses.</p><p><strong>Results: </strong>PRDX1 was identified as a key LRG significantly associated with BRCA risk (p_SMR = 0.0026). Single-cell RNA sequencing analysis revealed a significant upregulation of PRDX1 expression in monocytes, with enhanced cell-cell communication between PRDX1-positive monocytes and fibroblasts. Spatial transcriptomics analysis uncovered heterogeneous expression of PRDX1 in the tumor nest regions, highlighting the spatial interaction between PRDX1-positive monocytes and fibroblasts. The prognostic model constructed based on the gene expression profile of PRDX1-positive monocytes demonstrated high accuracy in predicting patient survival in both the training and validation cohorts. High-risk patients exhibited immune-suppressive microenvironment characteristics, including reduced immune cell infiltration and upregulation of immune checkpoint gene expression.</p><p><strong>Conclusion: </strong>This study reveals the key role of PRDX1 in BRCA progression, mainly through the regulation of the tumor microenvironment and immune escape mechanisms. The survival prediction model based on PRDX1 shows robust prognostic potential, and future research should focus on integrating PRDX1 with other biomarkers to enhance the precision of personalised medicine.</p>","PeriodicalId":12465,"journal":{"name":"Frontiers in Molecular Biosciences","volume":"12 ","pages":"1580622"},"PeriodicalIF":3.9,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12006012/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143977564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Editorial: Crosstalk between metabolism and immunity.","authors":"Jie Xiong","doi":"10.3389/fmolb.2025.1599219","DOIUrl":"https://doi.org/10.3389/fmolb.2025.1599219","url":null,"abstract":"","PeriodicalId":12465,"journal":{"name":"Frontiers in Molecular Biosciences","volume":"12 ","pages":"1599219"},"PeriodicalIF":3.9,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12003140/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144062408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Francesca Sciandra, Manuela Bozzi, Alina Witt, Paul Goffing, Sonia Covaceuszach, Sandra Blaess, Alberto Cassetta, Maria Giulia Bigotti, Thomas Huser, Andrea Brancaccio, Wolfgang Hübner
{"title":"Live cell optical super-resolution microscopy of dystroglycan mutants as a model for dystroglycanopathies in multiple cell lines.","authors":"Francesca Sciandra, Manuela Bozzi, Alina Witt, Paul Goffing, Sonia Covaceuszach, Sandra Blaess, Alberto Cassetta, Maria Giulia Bigotti, Thomas Huser, Andrea Brancaccio, Wolfgang Hübner","doi":"10.3389/fmolb.2025.1558170","DOIUrl":"https://doi.org/10.3389/fmolb.2025.1558170","url":null,"abstract":"<p><strong>Introduction: </strong>Dystroglycan (DG) is an adhesion complex comprising two subunits, α-DG and β-DG, which interact non-covalently at the plasma membrane. As a component of the dystrophin-glycoprotein complex DGC, DG plays a crucial role in linking the cytoskeleton to the surrounding basement membranes. Rare primary point mutations in the <i>DAG1</i> gene have been identified in patients with various forms of neuromuscular dystrophy, ranging in phenotype from mild to severe.</p><p><strong>Methods: </strong>To gain a deeper understanding of the molecular mechanisms underlying these pathologies, we have designed a series of chimeric GFP-tagged full-length α/β-DG constructs and expressed them in three different cell lines (U-2OS, HEK-293T and C2C12). Wild-type DG constructs were compared to their counterparts carrying pathologic missense mutations previously described in patients, namely, L84F, T190M and C667F and with the mutant I591D, i.e., the topological equivalent of V567D identified in zebrafish.</p><p><strong>Results: </strong>Live super-resolution fluorescence microscopy showed that the C667F mutant is retained within the ER/Golgi while the T190M and wild-type proteins are correctly localized to the plasma membrane in all 3 cell lines. The L84F mutant exhibits a delay in trafficking to the plasma membrane in two of the cell lines, while localizing strongly at the plasma membrane in the high-expression HEK-293T cells. Similarly, the I591D mutant accumulated at the plasma membrane in the HEK-293T cells, in contrast to the clear retention in the endoplasmic reticulum/Golgi apparatus observed in U-2OS and C2C12 cells.</p><p><strong>Discussion: </strong>Our data demonstrate the importance of using a range of different cell lines for a comprehensive study of DG mutants or variants by live cell optical super-resolution microscopy.</p>","PeriodicalId":12465,"journal":{"name":"Frontiers in Molecular Biosciences","volume":"12 ","pages":"1558170"},"PeriodicalIF":3.9,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12003124/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143984720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Paritaprevir as a pan-antiviral against different flaviviruses.","authors":"R P Yadav, N R Jena","doi":"10.3389/fmolb.2025.1524951","DOIUrl":"https://doi.org/10.3389/fmolb.2025.1524951","url":null,"abstract":"<p><strong>Introduction: </strong>The flavivirus infections caused by the Zika virus (ZIKV), Dengue virus (DENV), and West Nile virus (WNV) cause mild to serious pathological conditions, such as fever, joint pain, shock, internal bleeding, organ failure, nausea, breathlessness, brain tissue damage, neurodegenerative diseases, and deaths. As currently no efficient vaccine or drug is available to prevent or treat these diseases in humans, it is essential to identify potential drug-like molecules to treat these diseases. For these reasons, several known anti-viral drugs are repurposed against the proteases of ZIKV, WNV, and DENV to inhibit their activities.</p><p><strong>Methods: </strong>The GOLD 5.0 molecular docking program was used to dock 20 HIV and HCV drugs against the ZIKV protease. Based on docking scores, 5 drugs were found to bind to the ZIKV protease with high affinities. Subsequently, the AMBER ff14SB force field was employed to simulate these drug-bound complexes of ZIKV protease. The MM/PBSA free energy method was utilized to compute the binding free energies of these complexes. Consequently, the two best ZIKV protease inhibitors were repurposed against the proteases of DENV and WNV.</p><p><strong>Results and discussion: </strong>It is found that out of the 5 drugs, Ritonavir and Paritaprevir bind to the NS2B-NS3 protease of the ZIKV strongly with the Gibbs binding free energies (∆G<sub>bind</sub>) of -17.44±3.18 kcal/mol and -14.25±3.11 kcal/mol respectively. Remarkably, Ritonavir binds to the ZIKV Protease about 12 kcal/mol more strongly compared to its binding to the HIV protease. It is further found that Paritaprevir binds to DENV and WNV proteases as strongly as it binds to the ZIKV protease. Hence it is proposed that Paritaprevir may act as a potent pan-antiviral against the Zika, West Nile, and Dengue viral diseases.</p>","PeriodicalId":12465,"journal":{"name":"Frontiers in Molecular Biosciences","volume":"12 ","pages":"1524951"},"PeriodicalIF":3.9,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12003128/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143967615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Michael Hoffmann, Norbert Gottschalk, Philipp Huber, Vanessa Scheling, Nicole von Allmen, J Kolja Hegel
{"title":"Determination of sample stability for a broad panel of coagulation parameters and factor assays on the Cobas t 711 analyzer starting from fresh-never-frozen plasma.","authors":"Michael Hoffmann, Norbert Gottschalk, Philipp Huber, Vanessa Scheling, Nicole von Allmen, J Kolja Hegel","doi":"10.3389/fmolb.2025.1491239","DOIUrl":"https://doi.org/10.3389/fmolb.2025.1491239","url":null,"abstract":"<p><strong>Background: </strong>Preanalytical procedures can affect the accuracy of coagulation assay results. Recommended plasma storage temperatures and durations need to be defined for individual coagulation assays. Here, we evaluated the effect of commonly applied plasma storage conditions for a broad panel of 23 basic coagulation parameters as well as specialized factor assays developed for the Cobas<sup>®</sup> t 711 analyzer (Roche Diagnostics International Ltd., Rotkreuz, Switzerland).</p><p><strong>Methods: </strong>This single-center, prospective, observational study used anonymized, residual, platelet-poor plasma samples as well as pseudonymized plasma samples to obtain rare ranges of certain analytes. Fresh-never-frozen plasma samples processed within 4 h were tested in triplicate at time zero (t<sub>0</sub>), with measurements repeated at various predefined timepoints after storage at 18-25°C, 2-8°C, or under freezing and deep freezing. Mean deviation from t<sub>0</sub>, expressed as a percentage or as absolute change in signal at very low analyte levels, was assessed against predefined, assay-specific acceptance criteria for each analyte.</p><p><strong>Results: </strong>The sample stability results under the examined storage conditions for all 23 assays met or exceeded the requirements for routine laboratory coagulation testing and the respective acceptance criteria for each individual assay were fulfilled. Fresh-never-frozen samples were used to reflect real-life laboratory settings, enabling the early detection of out-of-specification results.</p><p><strong>Conclusion: </strong>Sample stability was determined for a broad panel of assays on the t 711 analyzer, for application in routine coagulation testing practice.</p>","PeriodicalId":12465,"journal":{"name":"Frontiers in Molecular Biosciences","volume":"12 ","pages":"1491239"},"PeriodicalIF":3.9,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12003954/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143996248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"LT-YOLO: long-term temporal enhanced YOLO for stenosis detection on invasive coronary angiography.","authors":"Jiaxin Li, Xiang Tang, Xuesong Wang","doi":"10.3389/fmolb.2025.1558495","DOIUrl":"https://doi.org/10.3389/fmolb.2025.1558495","url":null,"abstract":"<p><p>Coronary artery stenosis detection by invasive coronary angiography plays a pivotal role in computer-aided diagnosis and treatment. However, it faces the challenge of stenotic morphology confusion stemming from coronary-background similarity, varied morphology, and small-area stenoses. Furthermore, existing automated methods ignore long-temporal information mining. To address these limitations, this paper proposes a long-term temporal enhanced You Only Look Once (YOLO) method for automatic stenosis detection and assessment in invasive coronary angiography. Our approach integrates long-term temporal information and spatial information for stenosis detection with state-space models and YOLOv8. First, a spatial-aware backbone based on a dynamic Transformer and C2f Convolution of YOLOv8 combines the local and global feature extraction to distinguish the coronary regions from the background. Second, a spatial-temporal multi-level fusion neck integrates the long-term temporal and spatial features to handle varied stenotic morphology. Third, a detail-aware detection head leverages low-level information for accurate identification of small stenoses. Extensive experiments on 350 invasive coronary angiography (ICA) video sequences demonstrate the model's superior performance over seven state-of-the-art methods, particularly in detecting small stenoses (<50%), which were previously underexplored.</p>","PeriodicalId":12465,"journal":{"name":"Frontiers in Molecular Biosciences","volume":"12 ","pages":"1558495"},"PeriodicalIF":3.9,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12001240/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143976864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Corrigendum: Comprehensive landscape of junctional genes and their association with overall survival of patients with lung adenocarcinoma.","authors":"Bin Xie, Ting Wu, Duiguo Hong, Zhe Lu","doi":"10.3389/fmolb.2025.1583736","DOIUrl":"https://doi.org/10.3389/fmolb.2025.1583736","url":null,"abstract":"<p><p>[This corrects the article DOI: 10.3389/fmolb.2024.1380384.].</p>","PeriodicalId":12465,"journal":{"name":"Frontiers in Molecular Biosciences","volume":"12 ","pages":"1583736"},"PeriodicalIF":3.9,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11996649/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144004182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Lisa Becker, Jens Hausmann, Rieke Wellpott, Anna-Maria Hartmann
{"title":"Highly conserved ion binding sites are not all functionally relevant in mouse KCC4.","authors":"Lisa Becker, Jens Hausmann, Rieke Wellpott, Anna-Maria Hartmann","doi":"10.3389/fmolb.2025.1556250","DOIUrl":"https://doi.org/10.3389/fmolb.2025.1556250","url":null,"abstract":"<p><strong>Introduction: </strong>The potassium chloride cotransporter 4 (KCC4) is expressed in various tissues and plays an important role in distal renal acidification and hearing development. Although KCCs transport K<sup>+</sup> and Cl<sup>-</sup> in a 1:1 stoichiometry, two Cl<sup>-</sup> coordination sites were indicated via cryo-electron microscopy (CryoEM).</p><p><strong>Methods: </strong>In a comprehensive analysis, we analyzed here the consequences of point mutation of residues coordinating potassium, and chloride in the first (Cl<sub>1</sub>) and second (Cl<sub>2</sub>) coordinating site in KCC4 using Tl<sup>+</sup> based flux measurements.</p><p><strong>Results: </strong>Surprisingly, not all highly conserved coordination sites in KCC4 are essential. Three out of five residues (N<sup>131</sup>, Y<sup>216</sup>, and T<sup>432</sup>) are functionally relevant for potassium coordination. For chloride coordination in Cl<sub>1</sub>, all three residues (G<sup>134</sup>, V<sup>135</sup>, and I<sup>136</sup>) are important, whereas three out of four residues (G<sup>433</sup>, M<sup>435</sup>, and Y<sup>589</sup>) are relevant for chloride binding in Cl<sub>2</sub>. As all ion coordination sites are important in KCC2, this indicates that there is a certain flexibility in the stringency of ion coordination in KCC4. One possible reason for the different relevance of ion coordination sites could be the large extracellular loop (LEL). The LEL is structured differently within KCCs and is directly linked to the transmembrane domain (TM) 6, where most of the coordination sites reside. Substitution of ion coordination sites in the KCC2<sub>2-4-2</sub> chimera, in which the LEL from mouse KCC4 is exchanged with the LEL of rat KCC2, have the same effect as substitutions in rat KCC2. An exception is the substitution of the potassium coordination site I<sup>111</sup> in TM1, which shows enhanced activity in the KCC2<sub>2-4-2</sub> chimera compared to the impaired activity in rat KCC2 and not affected activity in mouse KCC4.</p><p><strong>Conclusion: </strong>Thus, the different relevance of the ion coordination sites between KCC2 and KCC4 cannot be attributed solely to the different structured LEL; other structural elements must also be involved here.</p>","PeriodicalId":12465,"journal":{"name":"Frontiers in Molecular Biosciences","volume":"12 ","pages":"1556250"},"PeriodicalIF":3.9,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11994965/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144011195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The role of CAFs in therapeutic resistance in triple negative breast cancer: an emerging challenge.","authors":"Marianna Rita Brogna, Valeria Varone, Michele DelSesto, Gerardo Ferrara","doi":"10.3389/fmolb.2025.1568865","DOIUrl":"https://doi.org/10.3389/fmolb.2025.1568865","url":null,"abstract":"<p><p>The tumor microenvironment (TME) is a crucial element of cancerous tissue and has emerged as a promising target for therapeutic strategies. The complex variety of stromal cells within the TME plays a vital role in determining the tumor's aggressiveness and its resistance to treatment. Tumor progression is not solely driven by cancer cells harboring genetic mutations but is also significantly influenced by non-cancerous host cells within the TME, which strongly impact tumor growth, metastasis, and the response to therapies. Cancer-associated fibroblasts (CAFs) are a diverse group of stromal cells within the TME. They play dual roles, both promoting and inhibiting tumor growth, making them intriguing targets for enhancing cancer therapies. Their significant contribution to creating a tumor-supportive environment has diminished the effectiveness of various cancer treatments, including radiation, chemotherapy, immunotherapy, and hormone therapy. Research has increasingly focused on understanding how CAFs contribute to therapy resistance in triple-negative breast cancer (TNBC) to improve treatment outcomes. However, the ways in which CAF patterns affect the TME and the response to immunotherapy in TNBC are not yet well understood and the interactions between CAFs, tumor cells, and immune cells in TNBC remain largely unexplored. In this review, we thoroughly exam ine the relationship between TNBC progression and CAF patterns. We discuss the current understanding of CAF heterogeneity, their role in tumor progression, and their impact on the tumor's response to therapeutic agents in TNBC. Additionally, we explore the potential and possible strategies for therapies targeting CAFs.</p>","PeriodicalId":12465,"journal":{"name":"Frontiers in Molecular Biosciences","volume":"12 ","pages":"1568865"},"PeriodicalIF":3.9,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11994926/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143987474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jun Xiao, Huimin Li, Xiaowei Li, Huifen Lei, Zhicai Li, Cuiying Li
{"title":"Establishment of a nomogram-based prognostic model (LASSO-Cox regression) for predicting platelet storage lesions under different storage conditions.","authors":"Jun Xiao, Huimin Li, Xiaowei Li, Huifen Lei, Zhicai Li, Cuiying Li","doi":"10.3389/fmolb.2025.1561114","DOIUrl":"https://doi.org/10.3389/fmolb.2025.1561114","url":null,"abstract":"<p><strong>Introduction: </strong>Platelet concentrates (PCs) are critical blood products used for transfusion, but stored platelets often experience quality deterioration, resulting in reduced efficacy post-transfusion. Currently, the lack of effective prediction models hinders the assessment of platelet storage quality. To address this, we developed a miRNA-based prognosis prediction model that comprehensively evaluates platelet quality under diverse storage conditions, offering valuable insights into platelet shelf life.</p><p><strong>Methods: </strong>We enrolled 249 eligible PC samples, divided into a training dataset and internal validation dataset (7:3). Through microRNA sequencing, we identified 13 differentially expressed miRNAs with platelets storage lesions (PSLs). Leveraging the LASSO-Cox regression model, we constructed a nomogram-based classifier based on the association between miRNA expression and the duration of PSLs-free survival. Performance evaluation using measures like concordance index, area under the curve, calibration curves, and decision curve analyses to confirm the model's robustness.</p><p><strong>Results: </strong>The nomogram classifier, incorporating miRNAs (miR-4485-3p, miR-12136, miR-25-5p, miR-148b-5p) and storage method, effectively categorized PCs into high-risk and low-risk groups. Notably, significant differences in PSLs-free survival were observed across all datasets, underscoring the precision and accuracy of our nomogram-based model.</p><p><strong>Discussion: </strong>This innovative classifier provides clinicians with a reliable tool to predict PSLs occurrence in PCs stored under different methods, facilitating improved clinical decision-making.</p>","PeriodicalId":12465,"journal":{"name":"Frontiers in Molecular Biosciences","volume":"12 ","pages":"1561114"},"PeriodicalIF":3.9,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11994887/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143984256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}